barnase [CMV Flexi Vector pFC14A (HaloTag 7)]
ribonuclease( domain architecture ID 10097120)
ribonuclease hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates
List of domain hits
Name | Accession | Description | Interval | E-value | |||
barnase | cd00933 | Barnase, a member of the family of homologous microbial ribonucleases, catalyses the cleavage ... |
5-111 | 4.69e-72 | |||
Barnase, a member of the family of homologous microbial ribonucleases, catalyses the cleavage of single-stranded RNA via a two-step mechanism thought to be similar to that of pancreatic ribonuclease. The mechanism involves a transesterification to give a 2', 3'-cyclic phosphate intermediate, followed by hydrolysis to yield a 3' nucleotide. The active site residues His and Glu act as general acid-base groups during catalysis, while the Arg and Lys residues are important in binding the reactive phosphate, the latter probably binding the phosphate in the transition state. Barstar, a small 89 residue intracellular protein is a natural inhibitor of Barnase. : Pssm-ID: 238471 [Multi-domain] Cd Length: 107 Bit Score: 209.67 E-value: 4.69e-72
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Name | Accession | Description | Interval | E-value | |||
barnase | cd00933 | Barnase, a member of the family of homologous microbial ribonucleases, catalyses the cleavage ... |
5-111 | 4.69e-72 | |||
Barnase, a member of the family of homologous microbial ribonucleases, catalyses the cleavage of single-stranded RNA via a two-step mechanism thought to be similar to that of pancreatic ribonuclease. The mechanism involves a transesterification to give a 2', 3'-cyclic phosphate intermediate, followed by hydrolysis to yield a 3' nucleotide. The active site residues His and Glu act as general acid-base groups during catalysis, while the Arg and Lys residues are important in binding the reactive phosphate, the latter probably binding the phosphate in the transition state. Barstar, a small 89 residue intracellular protein is a natural inhibitor of Barnase. Pssm-ID: 238471 [Multi-domain] Cd Length: 107 Bit Score: 209.67 E-value: 4.69e-72
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Ribonuclease | pfam00545 | ribonuclease; This enzyme hydrolyses RNA and oligoribonucleotides. |
27-109 | 5.05e-26 | |||
ribonuclease; This enzyme hydrolyses RNA and oligoribonucleotides. Pssm-ID: 459849 Cd Length: 81 Bit Score: 92.31 E-value: 5.05e-26
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Name | Accession | Description | Interval | E-value | |||
barnase | cd00933 | Barnase, a member of the family of homologous microbial ribonucleases, catalyses the cleavage ... |
5-111 | 4.69e-72 | |||
Barnase, a member of the family of homologous microbial ribonucleases, catalyses the cleavage of single-stranded RNA via a two-step mechanism thought to be similar to that of pancreatic ribonuclease. The mechanism involves a transesterification to give a 2', 3'-cyclic phosphate intermediate, followed by hydrolysis to yield a 3' nucleotide. The active site residues His and Glu act as general acid-base groups during catalysis, while the Arg and Lys residues are important in binding the reactive phosphate, the latter probably binding the phosphate in the transition state. Barstar, a small 89 residue intracellular protein is a natural inhibitor of Barnase. Pssm-ID: 238471 [Multi-domain] Cd Length: 107 Bit Score: 209.67 E-value: 4.69e-72
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Ribonuclease | pfam00545 | ribonuclease; This enzyme hydrolyses RNA and oligoribonucleotides. |
27-109 | 5.05e-26 | |||
ribonuclease; This enzyme hydrolyses RNA and oligoribonucleotides. Pssm-ID: 459849 Cd Length: 81 Bit Score: 92.31 E-value: 5.05e-26
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microbial_RNases | cd00389 | microbial_RNases. Ribonucleases (RNAses) cleave phosphodiester bonds in RNA and are essential ... |
38-104 | 2.11e-21 | |||
microbial_RNases. Ribonucleases (RNAses) cleave phosphodiester bonds in RNA and are essential for both non-specific RNA degradation and for numerous forms of RNA processing. The alignment contains fungal RNases (U2, T1, F1, Th, Pb, N1, and Ms) and bacterial RNases (barnase, binase, RNase Sa) , the majority of which are guanyl specific and fungal ribotoxins. Pssm-ID: 238228 Cd Length: 71 Bit Score: 80.36 E-value: 2.11e-21
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Blast search parameters | ||||
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