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GusA [Plant binary expression vector pCR3301]
Protein Classification
beta-glucuronidase ( domain architecture ID 11484589 )
beta-glucuronidase (GUS) catalyzes the conversion of beta-D-glucuronoside and water to an alcohol and D-glucuronate
List of domain hits
Name
Accession
Description
Interval
E-value
PRK10150
PRK10150 beta-D-glucuronidase; Provisional
11-608
0e+00
beta-D-glucuronidase; Provisional
Pssm-ID: 236657 [Multi-domain]
Cd Length: 604
Bit Score: 1172.86
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 11 R PVET P TREIK K L D GLWAF S LDRENCGIDQRWWESAL Q ESRA I AVPGSFNDQFADADIRNY A G N VWYQREVFIPKGWAGQ 90
Cdd:PRK10150 3 Y PVET K TREIK D L S GLWAF K LDRENCGIDQRWWESAL P ESRA M AVPGSFNDQFADADIRNY V G D VWYQREVFIPKGWAGQ 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 91 RIVLRF DA VTHY G KVWVN N QEVMEH Q GGYTPFEAD V TPYV I AGKSVRITVCVNNELNWQT I PPG M VI T D E NGKKKQ S Y FH 170
Cdd:PRK10150 83 RIVLRF GS VTHY A KVWVN G QEVMEH K GGYTPFEAD I TPYV Y AGKSVRITVCVNNELNWQT L PPG N VI E D G NGKKKQ K Y NF 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 171 DFFNYAGIHR S VMLYTTP N T WV DDITVVT HV AQD C NHASVDW Q V VA NGD --- VSV E LRDAD Q QVVATGQGTSGTLQVVNP 247
Cdd:PRK10150 163 DFFNYAGIHR P VMLYTTP K T HI DDITVVT EL AQD L NHASVDW S V ET NGD vds VSV T LRDAD G QVVATGQGTSGTLQVVNP 242
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 248 HLWQPGEGYLY E L E V T - AKS Q TE S D I YPLR V GIRSVAVKG Q QFLIN H KPFYF T GFG R HEDAD L RGKG F D N VL M VHDH A LM 326
Cdd:PRK10150 243 HLWQPGEGYLY T L C V E l AKS G TE C D T YPLR F GIRSVAVKG G QFLIN G KPFYF K GFG K HEDAD I RGKG L D E VL N VHDH N LM 322
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 327 D WIGANS Y RTSHYPY A EEMLD W AD E HGIVVIDET A AVG FQ LS L G I G F EAGNKPKE L YSEEAVNGETQQAHLQAI K ELIAR 406
Cdd:PRK10150 323 K WIGANS F RTSHYPY S EEMLD L AD R HGIVVIDET P AVG LN LS F G A G L EAGNKPKE T YSEEAVNGETQQAHLQAI R ELIAR 402
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 407 DKNHPSVVMWSIANEP DT R P QGAREYFAPLAE A TRKLDPTRP I TCVNVMF CDAH TDT I SDL F DVLCLNRYYGWYV Q SGDL 486
Cdd:PRK10150 403 DKNHPSVVMWSIANEP AS R E QGAREYFAPLAE L TRKLDPTRP V TCVNVMF ATPD TDT V SDL V DVLCLNRYYGWYV D SGDL 482
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 487 ETAEKVLEKELLAWQEKLH Q PIIITEYG V DTLAGLHSMY T DMWSEEYQCA W LDMYHRVFDRV S AVVGEQVWNFADFATSQ 566
Cdd:PRK10150 483 ETAEKVLEKELLAWQEKLH K PIIITEYG A DTLAGLHSMY D DMWSEEYQCA F LDMYHRVFDRV P AVVGEQVWNFADFATSQ 562
570 580 590 600
....*....|....*....|....*....|....*....|..
gi 548788774 567 GILRVGGNKKGIFTRDR K PKSAAFLL Q KRWTG MN FGEKPQ Q G 608
Cdd:PRK10150 563 GILRVGGNKKGIFTRDR Q PKSAAFLL K KRWTG IP FGEKPQ K G 604
Name
Accession
Description
Interval
E-value
PRK10150
PRK10150 beta-D-glucuronidase; Provisional
11-608
0e+00
beta-D-glucuronidase; Provisional
Pssm-ID: 236657 [Multi-domain]
Cd Length: 604
Bit Score: 1172.86
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 11 R PVET P TREIK K L D GLWAF S LDRENCGIDQRWWESAL Q ESRA I AVPGSFNDQFADADIRNY A G N VWYQREVFIPKGWAGQ 90
Cdd:PRK10150 3 Y PVET K TREIK D L S GLWAF K LDRENCGIDQRWWESAL P ESRA M AVPGSFNDQFADADIRNY V G D VWYQREVFIPKGWAGQ 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 91 RIVLRF DA VTHY G KVWVN N QEVMEH Q GGYTPFEAD V TPYV I AGKSVRITVCVNNELNWQT I PPG M VI T D E NGKKKQ S Y FH 170
Cdd:PRK10150 83 RIVLRF GS VTHY A KVWVN G QEVMEH K GGYTPFEAD I TPYV Y AGKSVRITVCVNNELNWQT L PPG N VI E D G NGKKKQ K Y NF 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 171 DFFNYAGIHR S VMLYTTP N T WV DDITVVT HV AQD C NHASVDW Q V VA NGD --- VSV E LRDAD Q QVVATGQGTSGTLQVVNP 247
Cdd:PRK10150 163 DFFNYAGIHR P VMLYTTP K T HI DDITVVT EL AQD L NHASVDW S V ET NGD vds VSV T LRDAD G QVVATGQGTSGTLQVVNP 242
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 248 HLWQPGEGYLY E L E V T - AKS Q TE S D I YPLR V GIRSVAVKG Q QFLIN H KPFYF T GFG R HEDAD L RGKG F D N VL M VHDH A LM 326
Cdd:PRK10150 243 HLWQPGEGYLY T L C V E l AKS G TE C D T YPLR F GIRSVAVKG G QFLIN G KPFYF K GFG K HEDAD I RGKG L D E VL N VHDH N LM 322
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 327 D WIGANS Y RTSHYPY A EEMLD W AD E HGIVVIDET A AVG FQ LS L G I G F EAGNKPKE L YSEEAVNGETQQAHLQAI K ELIAR 406
Cdd:PRK10150 323 K WIGANS F RTSHYPY S EEMLD L AD R HGIVVIDET P AVG LN LS F G A G L EAGNKPKE T YSEEAVNGETQQAHLQAI R ELIAR 402
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 407 DKNHPSVVMWSIANEP DT R P QGAREYFAPLAE A TRKLDPTRP I TCVNVMF CDAH TDT I SDL F DVLCLNRYYGWYV Q SGDL 486
Cdd:PRK10150 403 DKNHPSVVMWSIANEP AS R E QGAREYFAPLAE L TRKLDPTRP V TCVNVMF ATPD TDT V SDL V DVLCLNRYYGWYV D SGDL 482
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 487 ETAEKVLEKELLAWQEKLH Q PIIITEYG V DTLAGLHSMY T DMWSEEYQCA W LDMYHRVFDRV S AVVGEQVWNFADFATSQ 566
Cdd:PRK10150 483 ETAEKVLEKELLAWQEKLH K PIIITEYG A DTLAGLHSMY D DMWSEEYQCA F LDMYHRVFDRV P AVVGEQVWNFADFATSQ 562
570 580 590 600
....*....|....*....|....*....|....*....|..
gi 548788774 567 GILRVGGNKKGIFTRDR K PKSAAFLL Q KRWTG MN FGEKPQ Q G 608
Cdd:PRK10150 563 GILRVGGNKKGIFTRDR Q PKSAAFLL K KRWTG IP FGEKPQ K G 604
Glyco_hydro_2_C
pfam02836 Glycosyl hydrolases family 2, TIM barrel domain; This family contains beta-galactosidase, ...
282-601
4.84e-138
Glycosyl hydrolases family 2, TIM barrel domain; This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
Pssm-ID: 397119 [Multi-domain]
Cd Length: 302
Bit Score: 404.52
E-value: 4.84e-138
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 282 V A VK GQQ FLIN H KPFYF T G FG RHED A D L RG K GFD NV LMV H D HA LM DWIGA N SY RTSHYP YAE E MLDWA DE H GI V VIDE TA 361
Cdd:pfam02836 1 V E VK DGL FLIN G KPFYF R G VN RHED H D R RG R GFD MD LMV K D IQ LM KQNNI N AV RTSHYP NHP E WYQLC DE Y GI Y VIDE AN 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 362 AVGFQ L SLGI G f E AGN kpke L YSE EAV N G E TQQ AHL QAIK EL IA RDKNHPSV VM WS IA NE P dtrpq GA R E YF A PLAE AT R 441
Cdd:pfam02836 81 LETHG L WQKF G - E IEP ---- S YSE LTD N P E WLP AHL ERAE EL VQ RDKNHPSV II WS LG NE S ----- GA G E NI A AMYA AT K 150
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 442 K LDPTRP ITCVN vmfcdaht DT I SDLF D VLC L NR Y YGW Y VQS G DL E taek V L EK E L LA W QE K LHQ PIII T EYG VDTLA -- 519
Cdd:pfam02836 151 S LDPTRP VHYEG -------- VG I DPEV D DII L DI Y SRM Y EDY G HP E ---- V I EK Y L ED W YK K PQK PIII C EYG HAMGN sp 218
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 520 G LHSM Y T D MWS -- E EYQ CAWLDMY H RVFD -- R VSA V V GE QV W NFA DF ATSQGIL R VG GN kk G I F TR DR K PK S A A F L L Q KR 595
Cdd:pfam02836 219 G GLQE Y Q D LFY ky P EYQ GGFIWDW H DQGI qk R DPN V G GE WY W YGG DF GDRPSDY R FC GN -- G L F FA DR T PK P A L F E L K KR 296
....*.
gi 548788774 596 WTGMNF 601
Cdd:pfam02836 297 YWPIKI 302
LacZ
COG3250 Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism];
25-597
2.77e-116
Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism];
Pssm-ID: 442481 [Multi-domain]
Cd Length: 638
Bit Score: 360.23
E-value: 2.77e-116
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 25 G L W A F S L DRENC G IDQRWWE S ALQE sra I A VPG SFNDQFA ------ DADIRN Y A G NV WY Q R EVFI P KG W A G Q R IV L R F DA 98
Cdd:COG3250 1 G G W K F R L GDAPE G AKPDFDD S GWDP --- I T VPG DWELDLY glpdpf VGPWYL Y N G VG WY R R TFTV P AS W K G K R VF L H F EG 77
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 99 V THYGK VWVN NQE V ME H Q GG Y TPFE A D V T P Y VIA G KS V r IT V C V N N ELNWQTIPPG mvitdengkkkqsyfh D FFNYA GI 178
Cdd:COG3250 78 V DTAAE VWVN GKK V GY H E GG F TPFE F D I T D Y LKP G EN V - LA V R V D N PSDGSYLEGQ ---------------- D WWRTS GI 140
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 179 H R S V M L YT TP NTWVD D IT V VTHVAQD cn H A SVDWQ V VANGD ------ V S V E L R DAD QQ VVAT GQGT ----------- SG T 241
Cdd:COG3250 141 Y R D V W L EA TP KVHIE D VF V TPDLDDG -- S A TLTVE V ELENE sdagvt V E V T L L DAD GK VVAT ATAK vtlaageentv TL T 218
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 242 L Q V V NP H LW Q P GEGY LY E L E VT A K SQ - TES D IYPL R V G I R SVAVK G - QQ FL I N H KP FYFT G FG RHED ADLR G KGFDNVL M 319
Cdd:COG3250 219 L T V P NP K LW S P EDPN LY T L V VT L K DD g KVV D TVST R F G F R TIEID G d GG FL L N G KP VFLK G VN RHED WPDD G RAVTDEA M 298
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 320 VH D HA LM DWI G A N SY RTSHYP YAE E ML D WA DE H G IV V I DE TAAV gfqlsl GI G FEAGNK pkelyseeavng E TQQ A HLQA 399
Cdd:COG3250 299 RR D LE LM KEA G F N AV RTSHYP EDP E FY D LC DE L G LL V W DE APFE ------ WH G MLGDDP ------------ E FLE A VEAE 360
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 400 IK E LIA RD K NHPS VVM WS IA NE PDTR P qgare Y FA P L A E ATRK LDPTRP I tcvnvmfcdahtdtisdlfdvlclnryygw 479
Cdd:COG3250 361 LR E MVR RD R NHPS IIL WS GG NE SGGG P ----- N FA A L Y E WVKE LDPTRP V ------------------------------ 405
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 480 yvqsgdletaekvlekellawqeklhqp IIIT EYG VDTLAG L HSM Y TDM - WS EEYQ CA - W L DM Y HRV F D R VSAVV G EQV W 557
Cdd:COG3250 406 ---------------------------- RFLS EYG HAMPNS L GGG Y HQP s DF EEYQ AL q A L EE Y WEA F R R RPRLA G GFI W 457
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 548788774 558 NFA D FATSQGILRVGGNKK G IFT - R DR K PK S A AFLLQKR W T 597
Cdd:COG3250 458 QLN D YWPEPRDNDGNFCSW G LVD y Y DR T PK P A YYEVKSA W Q 498
GalB
NF041463 beta-galactosidase GalB;
73-478
1.70e-34
beta-galactosidase GalB;
Pssm-ID: 469351 [Multi-domain]
Cd Length: 799
Bit Score: 139.66
E-value: 1.70e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 73 G NV WY QREVF IP KGW AG QR I V L RF D AVTH Y GK VW V N N Q E V MEHQG GY TPFEA D V TPY VIA G KSVRITVCVN N ELN --- W Q 149
Cdd:NF041463 64 G VA WY RKKLD IP ASD AG KS I F L DI D GAMS Y AM VW L N G Q L V GGWPY GY NSWRL D L TPY LKP G GENQLAIRLD N PPE ssr W Y 143
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 150 tip PG mvitdengkkkqsyfhdffny A G IH R S V M L YT T pntwv DDI tvvt HVAQ ---------- DCNH A S VD WQ V - V A N G 218
Cdd:NF041463 144 --- PG --------------------- G G LY R N V W L TK T ----- NPV ---- HVAQ wgtfvttpev SADS A T VD LA V t V D N D 190
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 219 ------- D VS V E LRDA D ----------------- QQ V V A TGQG T - SG T L QVV NP H LW Q P G --- EGYL Y e LE VT AKS Q TES 270
Cdd:NF041463 191 saadadv E VS T E IYAL D adgkrtgkavarfapas LT V A A GESA T v SG S L TIA NP R LW G P P ptq TPNR Y - VA VT TVY Q GGK 269
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 271 -- D I Y PLRV GIRS V --- AVK G qq F L I N HKPFYFT G FGR H E D ADLR G KG F d NV lmvhd H A ------ LMDWI G A N SY R TS H Y 339
Cdd:NF041463 270 vv D R Y ETPF GIRS L rfd PDR G -- V L V N GEHIRLQ G VNQ H H D LGAL G AA F - NV ----- R A aerqle ILREM G C N AI R MA H N 341
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 340 P Y A E E M L DWA D EH G IV V I DE T aavgfqlslgig F EAGNKP K ELYSEEAVNGETQQAH L Q A ike L I A RD K NHPSV V MWSI A 419
Cdd:NF041463 342 P P A P E L L ELT D RM G FL V V DE I ------------ F DSWERK K TPLDFHLIFPDWHEQD L R A --- M I R RD R NHPSV I MWSI G 406
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 548788774 420 NE ------ PDTRPQG AR E yfap L AEATRKL DPTRP I T c VNVMFCDAHT d TISDLF DV LC LN r Y Y G 478
Cdd:NF041463 407 NE vgeqyt GEAGAAI AR R ---- L HDIVKEE DPTRP T T - ASMNYAKPDM - PFPAVM DV IS LN - Y Q G 464
Name
Accession
Description
Interval
E-value
PRK10150
PRK10150 beta-D-glucuronidase; Provisional
11-608
0e+00
beta-D-glucuronidase; Provisional
Pssm-ID: 236657 [Multi-domain]
Cd Length: 604
Bit Score: 1172.86
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 11 R PVET P TREIK K L D GLWAF S LDRENCGIDQRWWESAL Q ESRA I AVPGSFNDQFADADIRNY A G N VWYQREVFIPKGWAGQ 90
Cdd:PRK10150 3 Y PVET K TREIK D L S GLWAF K LDRENCGIDQRWWESAL P ESRA M AVPGSFNDQFADADIRNY V G D VWYQREVFIPKGWAGQ 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 91 RIVLRF DA VTHY G KVWVN N QEVMEH Q GGYTPFEAD V TPYV I AGKSVRITVCVNNELNWQT I PPG M VI T D E NGKKKQ S Y FH 170
Cdd:PRK10150 83 RIVLRF GS VTHY A KVWVN G QEVMEH K GGYTPFEAD I TPYV Y AGKSVRITVCVNNELNWQT L PPG N VI E D G NGKKKQ K Y NF 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 171 DFFNYAGIHR S VMLYTTP N T WV DDITVVT HV AQD C NHASVDW Q V VA NGD --- VSV E LRDAD Q QVVATGQGTSGTLQVVNP 247
Cdd:PRK10150 163 DFFNYAGIHR P VMLYTTP K T HI DDITVVT EL AQD L NHASVDW S V ET NGD vds VSV T LRDAD G QVVATGQGTSGTLQVVNP 242
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 248 HLWQPGEGYLY E L E V T - AKS Q TE S D I YPLR V GIRSVAVKG Q QFLIN H KPFYF T GFG R HEDAD L RGKG F D N VL M VHDH A LM 326
Cdd:PRK10150 243 HLWQPGEGYLY T L C V E l AKS G TE C D T YPLR F GIRSVAVKG G QFLIN G KPFYF K GFG K HEDAD I RGKG L D E VL N VHDH N LM 322
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 327 D WIGANS Y RTSHYPY A EEMLD W AD E HGIVVIDET A AVG FQ LS L G I G F EAGNKPKE L YSEEAVNGETQQAHLQAI K ELIAR 406
Cdd:PRK10150 323 K WIGANS F RTSHYPY S EEMLD L AD R HGIVVIDET P AVG LN LS F G A G L EAGNKPKE T YSEEAVNGETQQAHLQAI R ELIAR 402
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 407 DKNHPSVVMWSIANEP DT R P QGAREYFAPLAE A TRKLDPTRP I TCVNVMF CDAH TDT I SDL F DVLCLNRYYGWYV Q SGDL 486
Cdd:PRK10150 403 DKNHPSVVMWSIANEP AS R E QGAREYFAPLAE L TRKLDPTRP V TCVNVMF ATPD TDT V SDL V DVLCLNRYYGWYV D SGDL 482
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 487 ETAEKVLEKELLAWQEKLH Q PIIITEYG V DTLAGLHSMY T DMWSEEYQCA W LDMYHRVFDRV S AVVGEQVWNFADFATSQ 566
Cdd:PRK10150 483 ETAEKVLEKELLAWQEKLH K PIIITEYG A DTLAGLHSMY D DMWSEEYQCA F LDMYHRVFDRV P AVVGEQVWNFADFATSQ 562
570 580 590 600
....*....|....*....|....*....|....*....|..
gi 548788774 567 GILRVGGNKKGIFTRDR K PKSAAFLL Q KRWTG MN FGEKPQ Q G 608
Cdd:PRK10150 563 GILRVGGNKKGIFTRDR Q PKSAAFLL K KRWTG IP FGEKPQ K G 604
Glyco_hydro_2_C
pfam02836 Glycosyl hydrolases family 2, TIM barrel domain; This family contains beta-galactosidase, ...
282-601
4.84e-138
Glycosyl hydrolases family 2, TIM barrel domain; This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
Pssm-ID: 397119 [Multi-domain]
Cd Length: 302
Bit Score: 404.52
E-value: 4.84e-138
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 282 V A VK GQQ FLIN H KPFYF T G FG RHED A D L RG K GFD NV LMV H D HA LM DWIGA N SY RTSHYP YAE E MLDWA DE H GI V VIDE TA 361
Cdd:pfam02836 1 V E VK DGL FLIN G KPFYF R G VN RHED H D R RG R GFD MD LMV K D IQ LM KQNNI N AV RTSHYP NHP E WYQLC DE Y GI Y VIDE AN 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 362 AVGFQ L SLGI G f E AGN kpke L YSE EAV N G E TQQ AHL QAIK EL IA RDKNHPSV VM WS IA NE P dtrpq GA R E YF A PLAE AT R 441
Cdd:pfam02836 81 LETHG L WQKF G - E IEP ---- S YSE LTD N P E WLP AHL ERAE EL VQ RDKNHPSV II WS LG NE S ----- GA G E NI A AMYA AT K 150
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 442 K LDPTRP ITCVN vmfcdaht DT I SDLF D VLC L NR Y YGW Y VQS G DL E taek V L EK E L LA W QE K LHQ PIII T EYG VDTLA -- 519
Cdd:pfam02836 151 S LDPTRP VHYEG -------- VG I DPEV D DII L DI Y SRM Y EDY G HP E ---- V I EK Y L ED W YK K PQK PIII C EYG HAMGN sp 218
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 520 G LHSM Y T D MWS -- E EYQ CAWLDMY H RVFD -- R VSA V V GE QV W NFA DF ATSQGIL R VG GN kk G I F TR DR K PK S A A F L L Q KR 595
Cdd:pfam02836 219 G GLQE Y Q D LFY ky P EYQ GGFIWDW H DQGI qk R DPN V G GE WY W YGG DF GDRPSDY R FC GN -- G L F FA DR T PK P A L F E L K KR 296
....*.
gi 548788774 596 WTGMNF 601
Cdd:pfam02836 297 YWPIKI 302
LacZ
COG3250 Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism];
25-597
2.77e-116
Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism];
Pssm-ID: 442481 [Multi-domain]
Cd Length: 638
Bit Score: 360.23
E-value: 2.77e-116
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 25 G L W A F S L DRENC G IDQRWWE S ALQE sra I A VPG SFNDQFA ------ DADIRN Y A G NV WY Q R EVFI P KG W A G Q R IV L R F DA 98
Cdd:COG3250 1 G G W K F R L GDAPE G AKPDFDD S GWDP --- I T VPG DWELDLY glpdpf VGPWYL Y N G VG WY R R TFTV P AS W K G K R VF L H F EG 77
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 99 V THYGK VWVN NQE V ME H Q GG Y TPFE A D V T P Y VIA G KS V r IT V C V N N ELNWQTIPPG mvitdengkkkqsyfh D FFNYA GI 178
Cdd:COG3250 78 V DTAAE VWVN GKK V GY H E GG F TPFE F D I T D Y LKP G EN V - LA V R V D N PSDGSYLEGQ ---------------- D WWRTS GI 140
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 179 H R S V M L YT TP NTWVD D IT V VTHVAQD cn H A SVDWQ V VANGD ------ V S V E L R DAD QQ VVAT GQGT ----------- SG T 241
Cdd:COG3250 141 Y R D V W L EA TP KVHIE D VF V TPDLDDG -- S A TLTVE V ELENE sdagvt V E V T L L DAD GK VVAT ATAK vtlaageentv TL T 218
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 242 L Q V V NP H LW Q P GEGY LY E L E VT A K SQ - TES D IYPL R V G I R SVAVK G - QQ FL I N H KP FYFT G FG RHED ADLR G KGFDNVL M 319
Cdd:COG3250 219 L T V P NP K LW S P EDPN LY T L V VT L K DD g KVV D TVST R F G F R TIEID G d GG FL L N G KP VFLK G VN RHED WPDD G RAVTDEA M 298
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 320 VH D HA LM DWI G A N SY RTSHYP YAE E ML D WA DE H G IV V I DE TAAV gfqlsl GI G FEAGNK pkelyseeavng E TQQ A HLQA 399
Cdd:COG3250 299 RR D LE LM KEA G F N AV RTSHYP EDP E FY D LC DE L G LL V W DE APFE ------ WH G MLGDDP ------------ E FLE A VEAE 360
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 400 IK E LIA RD K NHPS VVM WS IA NE PDTR P qgare Y FA P L A E ATRK LDPTRP I tcvnvmfcdahtdtisdlfdvlclnryygw 479
Cdd:COG3250 361 LR E MVR RD R NHPS IIL WS GG NE SGGG P ----- N FA A L Y E WVKE LDPTRP V ------------------------------ 405
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 480 yvqsgdletaekvlekellawqeklhqp IIIT EYG VDTLAG L HSM Y TDM - WS EEYQ CA - W L DM Y HRV F D R VSAVV G EQV W 557
Cdd:COG3250 406 ---------------------------- RFLS EYG HAMPNS L GGG Y HQP s DF EEYQ AL q A L EE Y WEA F R R RPRLA G GFI W 457
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 548788774 558 NFA D FATSQGILRVGGNKK G IFT - R DR K PK S A AFLLQKR W T 597
Cdd:COG3250 458 QLN D YWPEPRDNDGNFCSW G LVD y Y DR T PK P A YYEVKSA W Q 498
Glyco_hydro_2_N
pfam02837 Glycosyl hydrolases family 2, sugar binding domain; This family contains beta-galactosidase, ...
20-188
2.59e-75
Glycosyl hydrolases family 2, sugar binding domain; This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
Pssm-ID: 397120 [Multi-domain]
Cd Length: 169
Bit Score: 237.91
E-value: 2.59e-75
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 20 IK K L D G L WAF S L DREN CG ID Q R WWESALQESR A IAVP G S F NDQ ----------- FAD AD I RN Y A G NV WYQR EV FIP KG WA 88
Cdd:pfam02837 1 IK S L N G E WAF A L FDAP CG AP Q S WWESALQESR T IAVP S S W NDQ piytnveypid FAD PF I PT Y N G TG WYQR TF FIP SK WA 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 89 GQRI V LRFD A VTHYG K VWVN N Q E V M EHQGGYTPFE A D V TPYVIAGK S v RI T V C V nne LNW Q tip P G MV I T D E NGK kkqs Y 168
Cdd:pfam02837 81 GQRI R LRFD G VTHYG E VWVN G Q W V G EHQGGYTPFE F D L TPYVIAGK N - RI A V K V --- LNW S --- D G *Y I E D Q NGK ---- Y 149
170 180
....*....|....*....|
gi 548788774 169 FHDF F NY A GI H R S V M L Y TTP 188
Cdd:pfam02837 150 FHDF W NY S GI Y R D V S L L TTP 169
ebgA
PRK10340 cryptic beta-D-galactosidase subunit alpha; Reviewed
77-574
2.48e-46
cryptic beta-D-galactosidase subunit alpha; Reviewed
Pssm-ID: 236673 [Multi-domain]
Cd Length: 1021
Bit Score: 176.02
E-value: 2.48e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 77 YQR EVFIPK GW A G QRIVLR FD A V TH Y GK V W VN N Q E V MEHQ G GYTPF E A D VTPY V IA G KSV r IT V C V nne LN W QT ipp GMV 156
Cdd:PRK10340 113 YQR TFTLSD GW Q G KQTIIK FD G V ET Y FE V Y VN G Q Y V GFSK G SRLTA E F D ISAM V KT G DNL - LC V R V --- MQ W AD --- STY 185
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 157 IT D E ngkkkqsyfh D FFNY AGI H R S V M L YTT P N T WVD D I TV V T HVAQ D CNH A SVDWQ VV ANGDV ------ SV E LRDA D QQ 230
Cdd:PRK10340 186 LE D Q ---------- D MWWL AGI F R D V Y L VGK P L T HIN D F TV R T DFDE D YCD A TLSCE VV LENLA aspvvt TL E YTLF D GE 255
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 231 - VV ATGQGTSGTLQ ---------- V VN P HL W QPGEG YLY E L EV T A K - SQTES - DIY P L RVG I R SVA V KGQQ F L IN HKPFY 297
Cdd:PRK10340 256 r VV HSSAIDHLAIE kltsasfaft V EQ P QQ W SAESP YLY H L VM T L K d ANGNV l EVV P Q RVG F R DIK V RDGL F W IN NRYVK 335
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 298 FT G FG RH EDADLR G KGFDNVLMVH D HA LM DWIGA NS Y RT S HYP YAEEMLDWA D EH G IV V ID ET -- AAV GF QLS lgigfea 375
Cdd:PRK10340 336 LH G VN RH DNDHRK G RAVGMDRVEK D IQ LM KQHNI NS V RT A HYP NDPRFYELC D IY G LF V MA ET dv ESH GF ANV ------- 408
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 376 gnkpk ELY S EEAVNGETQQAHLQA I KEL I ARD KNHPS VVM WS IA NE pdtrp Q G AREYFAPLAE A TRK LD P TR PI tcvnvm 455
Cdd:PRK10340 409 ----- GDI S RITDDPQWEKVYVDR I VRH I HAQ KNHPS III WS LG NE ----- S G YGCNIRAMYH A AKA LD D TR LV ------ 472
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 456 fcda H --------- T D T IS DLFDVLC L NRYY G W Y vqsgdletaekvlekellawqe KLHQ P I I IT EY G vdtlagl H S M YT 526
Cdd:PRK10340 473 ---- H yeedrdaev V D V IS TMYTRVE L MNEF G E Y ---------------------- PHPK P R I LC EY A ------- H A M GN 519
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 548788774 527 DMWS - E EYQ cawldmyh R VF DRVSAVV G EQ VW NFA D fats Q GI LRVGG N 574
Cdd:PRK10340 520 GPGG l T EYQ -------- N VF YKHDCIQ G HY VW EWC D ---- H GI QAQDD N 556
lacZ
PRK09525 beta-galactosidase;
15-421
9.33e-36
beta-galactosidase;
Pssm-ID: 236548 [Multi-domain]
Cd Length: 1027
Bit Score: 143.92
E-value: 9.33e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 15 T P TREIKK L D G L W A FS LDRENCGIDQR W W E SA L QESRA I A VP GSFNDQFA DA D I rn Y A g NV ------------------ W 76
Cdd:PRK09525 47 R P SQQRQS L N G E W R FS YFPAPEAVPES W L E CD L PDADT I P VP SNWQLHGY DA P I -- Y T - NV typipvnppfvpeenptg C 123
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 77 Y QREVFIPKG W -- A GQ - RI V lr FD A V THYGKV W V N NQE V mehqg GY T ----- P F E A D VT P YVI AG KS v R IT V C V nne L N W 148
Cdd:PRK09525 124 Y SLTFTVDES W lq S GQ t RI I -- FD G V NSAFHL W C N GRW V ----- GY S qdsrl P A E F D LS P FLR AG EN - R LA V M V --- L R W 192
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 149 Q tippgmvitdengkk KQ SY F -- H D FFNYA GI H R S V M L YTT P N T WVD D ITVV T HVAQ D CNH A SVDWQVVA NG D ------ V 220
Cdd:PRK09525 193 S --------------- DG SY L ed Q D MWRMS GI F R D V S L LHK P T T QLS D FHIT T ELDD D FRR A VLEVEAQV NG E lrdelr V 257
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 221 S V E L R D ADQQ V VATGQG --------------- TSGT L Q V V NP H LW QPGEGY LY ELE V T -- AKSQ T -- E SDI Y P lr VG I R S 281
Cdd:PRK09525 258 T V Q L W D GETL V ASGTAP fgteiidergayadr VTLR L N V E NP K LW SAETPN LY RAV V S ll DADG T li E AEA Y D -- VG F R K 335
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 282 V AVKGQQFLI N H KP FYFT G FG RHE DADLR G KGF D NVL MV H D HA LM DWIGA N SY R T SHYP YAEEMLDWA D EH G IV V I DE t A 361
Cdd:PRK09525 336 V EIENGLLKL N G KP LLIR G VN RHE HHPEH G QVM D EET MV Q D IL LM KQHNF N AV R C SHYP NHPLWYELC D RY G LY V V DE - A 414
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 548788774 362 AV --- G FQ ---- LS lgigfeag NK P KE L yseeavngetq Q A HLQAIKELIA RD K NHPS VVM WS IA NE 421
Cdd:PRK09525 415 NI eth G MV pmnr LS -------- DD P RW L ----------- P A MSERVTRMVQ RD R NHPS III WS LG NE 462
GalB
NF041463 beta-galactosidase GalB;
73-478
1.70e-34
beta-galactosidase GalB;
Pssm-ID: 469351 [Multi-domain]
Cd Length: 799
Bit Score: 139.66
E-value: 1.70e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 73 G NV WY QREVF IP KGW AG QR I V L RF D AVTH Y GK VW V N N Q E V MEHQG GY TPFEA D V TPY VIA G KSVRITVCVN N ELN --- W Q 149
Cdd:NF041463 64 G VA WY RKKLD IP ASD AG KS I F L DI D GAMS Y AM VW L N G Q L V GGWPY GY NSWRL D L TPY LKP G GENQLAIRLD N PPE ssr W Y 143
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 150 tip PG mvitdengkkkqsyfhdffny A G IH R S V M L YT T pntwv DDI tvvt HVAQ ---------- DCNH A S VD WQ V - V A N G 218
Cdd:NF041463 144 --- PG --------------------- G G LY R N V W L TK T ----- NPV ---- HVAQ wgtfvttpev SADS A T VD LA V t V D N D 190
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 219 ------- D VS V E LRDA D ----------------- QQ V V A TGQG T - SG T L QVV NP H LW Q P G --- EGYL Y e LE VT AKS Q TES 270
Cdd:NF041463 191 saadadv E VS T E IYAL D adgkrtgkavarfapas LT V A A GESA T v SG S L TIA NP R LW G P P ptq TPNR Y - VA VT TVY Q GGK 269
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 271 -- D I Y PLRV GIRS V --- AVK G qq F L I N HKPFYFT G FGR H E D ADLR G KG F d NV lmvhd H A ------ LMDWI G A N SY R TS H Y 339
Cdd:NF041463 270 vv D R Y ETPF GIRS L rfd PDR G -- V L V N GEHIRLQ G VNQ H H D LGAL G AA F - NV ----- R A aerqle ILREM G C N AI R MA H N 341
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 340 P Y A E E M L DWA D EH G IV V I DE T aavgfqlslgig F EAGNKP K ELYSEEAVNGETQQAH L Q A ike L I A RD K NHPSV V MWSI A 419
Cdd:NF041463 342 P P A P E L L ELT D RM G FL V V DE I ------------ F DSWERK K TPLDFHLIFPDWHEQD L R A --- M I R RD R NHPSV I MWSI G 406
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 548788774 420 NE ------ PDTRPQG AR E yfap L AEATRKL DPTRP I T c VNVMFCDAHT d TISDLF DV LC LN r Y Y G 478
Cdd:NF041463 407 NE vgeqyt GEAGAAI AR R ---- L HDIVKEE DPTRP T T - ASMNYAKPDM - PFPAVM DV IS LN - Y Q G 464
Glyco_hydro_2
pfam00703 Glycosyl hydrolases family 2; This family contains beta-galactosidase, beta-mannosidase and ...
192-280
2.01e-08
Glycosyl hydrolases family 2; This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
Pssm-ID: 395572 [Multi-domain]
Cd Length: 106
Bit Score: 52.48
E-value: 2.01e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548788774 192 VD D ITVVTHVAQ D c NH A S V DWQ V VANG D -------- VSV E LR DAD ------- QQ V VAT G Q G TSGT L Q V V NP H LW Q P GEGY 256
Cdd:pfam00703 3 IE D VFITPDLDD D - KT A K V TVE V ELEN D gdasvevt LET E IK DAD gktvaaa AK V LVL G A G ETTE L E V K NP K LW S P ETPN 81
90 100
....*....|....*....|....*
gi 548788774 257 LY E L E V T - A K SQTES D IYPL R V G I R 280
Cdd:pfam00703 82 LY T L T V E l D K DGKVI D EVST R F G F R 106
Blast search parameters
Data Source:
Precalculated data, version = cdd.v.3.21
Preset Options: Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
