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Conserved domains on  [gi|675803261|gb|AIL94219|]
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membrane protein [Bat Hp-betacoronavirus/Zhejiang2013]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
betaCoV_M cd21565
betacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein ...
 14-222 6.15e-118

betacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein of betacoronaviruses including the M proteins from three highly pathogenic human coronaviruses (CoVs) such as Middle East respiratory syndrome (MERS)-related CoV, severe acute respiratory syndrome (SARS) CoV, and SARS-CoV-2, also known as 2019 novel CoV (2019-nCoV) or COVID-19 virus. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


:

Pssm-ID: 394851  Cd Length: 208  Bit Score: 334.35  E-value: 6.15e-118
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  14 LFKVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAIYHVNVVFMGFAITFAIIVGCMW 93
Cdd:cd21565    1 IIAIIKDWNFAWSIIFLLITIVLQYGYPSRSMTVYVIKMFVLWLLWPLSIALSVFAAVYPINLVSQGISGIFAAISALMW 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  94 LGYWIASIRLFRRTGSVWSFNPETNRLLNVAIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGRADLESLPNEITVAT 173
Cdd:cd21565   81 LSYFVQSIRLFRRTGSWWSFNPETNCLLNVPIGGTTVVRPLVEDSTSITAVVTRGHLKMAGMHLGRCDYDRLPNEVTVAK 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 675803261 174 SRTLSYFKMSRKVNIGVGSGVATYLRYKVGNHRVPNArASEDQEDLLVV 222
Cdd:cd21565  161 PNVLIAYKMVKRQRYGTNSGVAIYHRYKAGNYRVPPT-ITSDDELALLR 208
 
Name Accession Description Interval E-value
betaCoV_M cd21565
betacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein ...
 14-222 6.15e-118

betacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein of betacoronaviruses including the M proteins from three highly pathogenic human coronaviruses (CoVs) such as Middle East respiratory syndrome (MERS)-related CoV, severe acute respiratory syndrome (SARS) CoV, and SARS-CoV-2, also known as 2019 novel CoV (2019-nCoV) or COVID-19 virus. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394851  Cd Length: 208  Bit Score: 334.35  E-value: 6.15e-118
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  14 LFKVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAIYHVNVVFMGFAITFAIIVGCMW 93
Cdd:cd21565    1 IIAIIKDWNFAWSIIFLLITIVLQYGYPSRSMTVYVIKMFVLWLLWPLSIALSVFAAVYPINLVSQGISGIFAAISALMW 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  94 LGYWIASIRLFRRTGSVWSFNPETNRLLNVAIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGRADLESLPNEITVAT 173
Cdd:cd21565   81 LSYFVQSIRLFRRTGSWWSFNPETNCLLNVPIGGTTVVRPLVEDSTSITAVVTRGHLKMAGMHLGRCDYDRLPNEVTVAK 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 675803261 174 SRTLSYFKMSRKVNIGVGSGVATYLRYKVGNHRVPNArASEDQEDLLVV 222
Cdd:cd21565  161 PNVLIAYKMVKRQRYGTNSGVAIYHRYKAGNYRVPPT-ITSDDELALLR 208
CoV_M pfam01635
Coronavirus M matrix/glycoprotein; This family consists of various coronavirus matrix proteins ...
 16-219 2.87e-92

Coronavirus M matrix/glycoprotein; This family consists of various coronavirus matrix proteins which are transmembrane glycoproteins. The membrane (M) protein is the most abundant structural protein and defines the shape of the viral envelope, being the central organizer of coronavirus assembly. M proteins play a critical role in protein-protein interactions (as well as protein-RNA interactions) since virus-like particle (VLP) formation in many CoVs requires only the M and envelope (E) proteins for efficient virion assembly. Interaction of spike (S) with M is necessary for retention of S in the ER-Golgi intermediate compartment (ERGIC)/Golgi complex and its incorporation into new virions; binding of M to nucleocapsid (N) proteins stabilizes the nucleocapsid. Together, M and E protein make up the viral envelope and their interaction is sufficient for the production and release of virus-like particles (VLPs).


Pssm-ID: 460275  Cd Length: 208  Bit Score: 269.15  E-value: 2.87e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261   16 KVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAI--YHVNVVFMGFAITFAIIVGCMW 93
Cdd:pfam01635   1 SHLREWNFAWSIILTLLIVVLQYGYASRSRFLYVLKMILLWLLWPLTLALSIFNAVasYNVNYVYFGFSIIFAVVTFVLW 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261   94 LGYWIASIRLFRRTGSVWSFNPETNRLLNVAIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGR-ADLESLPNEITVA 172
Cdd:pfam01635  81 LMYFVNSIRLYRRTGSWWSFNPETNALLCVSVLGRTVTRPLDEDPTGITLTLLSGTLYVEGQKLARgVSVESLPNYVTVA 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 675803261  173 TSRTLS-YFKMSRKVNIGVGSGVATYLRYKVGNHRVPNARASEDQEDL 219
Cdd:pfam01635 161 KPSTTIhYLKKGKSDNVGSNTGWAIYVRSKAGNYSLPSTTTGKLEESA 208
 
Name Accession Description Interval E-value
betaCoV_M cd21565
betacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein ...
 14-222 6.15e-118

betacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein of betacoronaviruses including the M proteins from three highly pathogenic human coronaviruses (CoVs) such as Middle East respiratory syndrome (MERS)-related CoV, severe acute respiratory syndrome (SARS) CoV, and SARS-CoV-2, also known as 2019 novel CoV (2019-nCoV) or COVID-19 virus. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394851  Cd Length: 208  Bit Score: 334.35  E-value: 6.15e-118
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  14 LFKVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAIYHVNVVFMGFAITFAIIVGCMW 93
Cdd:cd21565    1 IIAIIKDWNFAWSIIFLLITIVLQYGYPSRSMTVYVIKMFVLWLLWPLSIALSVFAAVYPINLVSQGISGIFAAISALMW 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  94 LGYWIASIRLFRRTGSVWSFNPETNRLLNVAIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGRADLESLPNEITVAT 173
Cdd:cd21565   81 LSYFVQSIRLFRRTGSWWSFNPETNCLLNVPIGGTTVVRPLVEDSTSITAVVTRGHLKMAGMHLGRCDYDRLPNEVTVAK 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 675803261 174 SRTLSYFKMSRKVNIGVGSGVATYLRYKVGNHRVPNArASEDQEDLLVV 222
Cdd:cd21565  161 PNVLIAYKMVKRQRYGTNSGVAIYHRYKAGNYRVPPT-ITSDDELALLR 208
CoV_M pfam01635
Coronavirus M matrix/glycoprotein; This family consists of various coronavirus matrix proteins ...
 16-219 2.87e-92

Coronavirus M matrix/glycoprotein; This family consists of various coronavirus matrix proteins which are transmembrane glycoproteins. The membrane (M) protein is the most abundant structural protein and defines the shape of the viral envelope, being the central organizer of coronavirus assembly. M proteins play a critical role in protein-protein interactions (as well as protein-RNA interactions) since virus-like particle (VLP) formation in many CoVs requires only the M and envelope (E) proteins for efficient virion assembly. Interaction of spike (S) with M is necessary for retention of S in the ER-Golgi intermediate compartment (ERGIC)/Golgi complex and its incorporation into new virions; binding of M to nucleocapsid (N) proteins stabilizes the nucleocapsid. Together, M and E protein make up the viral envelope and their interaction is sufficient for the production and release of virus-like particles (VLPs).


Pssm-ID: 460275  Cd Length: 208  Bit Score: 269.15  E-value: 2.87e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261   16 KVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAI--YHVNVVFMGFAITFAIIVGCMW 93
Cdd:pfam01635   1 SHLREWNFAWSIILTLLIVVLQYGYASRSRFLYVLKMILLWLLWPLTLALSIFNAVasYNVNYVYFGFSIIFAVVTFVLW 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261   94 LGYWIASIRLFRRTGSVWSFNPETNRLLNVAIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGR-ADLESLPNEITVA 172
Cdd:pfam01635  81 LMYFVNSIRLYRRTGSWWSFNPETNALLCVSVLGRTVTRPLDEDPTGITLTLLSGTLYVEGQKLARgVSVESLPNYVTVA 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 675803261  173 TSRTLS-YFKMSRKVNIGVGSGVATYLRYKVGNHRVPNARASEDQEDL 219
Cdd:pfam01635 161 KPSTTIhYLKKGKSDNVGSNTGWAIYVRSKAGNYSLPSTTTGKLEESA 208
SARS-like-CoV_M cd21569
Membrane (or Matrix) protein from Severe acute respiratory syndrome (SARS) coronavirus, ...
 6-222 3.40e-74

Membrane (or Matrix) protein from Severe acute respiratory syndrome (SARS) coronavirus, SARS-CoV-2, and related betacoronaviruses in the B lineage; This group contains the Membrane (M) protein of Severe acute respiratory syndrome coronavirus (SARS-CoV), SARS-CoV-2 (also known as 2019 novel CoV (2019-nCoV) or COVID-19 virus), and related proteins from betacoronaviruses in the sarbecovirus subgenera (B lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394855  Cd Length: 218  Bit Score: 223.73  E-value: 3.40e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261   6 NNTITQEELFKVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAIYHVNVVFMGFAITF 85
Cdd:cd21569    1 NGTITVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAM 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  86 AIIVGCMWLGYWIASIRLFRRTGSVWSFNPETNRLLNVAIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGRADLESL 165
Cdd:cd21569   81 ACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTILTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDL 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 675803261 166 PNEITVATSRTLSYFKMSRKVNIGVGSGVATYLRYKVGNHRVPNARASEDQEDLLVV 222
Cdd:cd21569  161 PKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHSGSNDNIALLV 217
batCoV_HKU9-like_M cd21570
Membrane (or Matrix) protein from bat coronavirus HKU9 and related betacoronaviruses in the D ...
 6-216 4.92e-68

Membrane (or Matrix) protein from bat coronavirus HKU9 and related betacoronaviruses in the D lineage; This group contains the Membrane (M) protein of Rousettus bat coronavirus HKU9, and similar proteins from betacoronaviruses in the nobecovirus subgenera (D lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394856  Cd Length: 217  Bit Score: 208.17  E-value: 4.92e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261   6 NNTITQEELFKVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAIYHVNVVFMGFAITF 85
Cdd:cd21570    1 NSTFSREEVFAAVKDWNFAVSVLLLFITILLQWGYPSRCKPLWVVKMFILWLLWPLSIAAAVFAAVYPINKVAFGFAIAF 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  86 AIIVGCMWLGYWIASIRLFRRTGSVWSFNPETNRLLNVAIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGRADLESL 165
Cdd:cd21570   81 ACISGLMWLSYFISSFRLLCRTGSAWSFMPETNMLLNVPLLGRTVTRPILADSPAIQFLLLRGEIQFDGFKLGRCDPGEM 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 675803261 166 PNEITVATSRTLSYFKMSRKVNIGVGSGVATYLRYKVGNHRVPNARASEDQ 216
Cdd:cd21570  161 PDIVTVAKPASLHWYKKALTRNIGPKSAIIVYIKYKVGNHRVQNTNEDGER 211
MERS-like-CoV_M cd21567
Membrane (or Matrix) protein from Middle East respiratory syndrome-related coronavirus and ...
 5-215 8.29e-68

Membrane (or Matrix) protein from Middle East respiratory syndrome-related coronavirus and related betacoronaviruses in the C lineage; This group contains the Membrane (M) protein of Middle East respiratory syndrome (MERS)-related CoV, bat-CoV HKU5, and similar proteins from betacoronaviruses in the merbecovirus subgenera (C lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394853  Cd Length: 216  Bit Score: 207.43  E-value: 8.29e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261   5 SNNTITQEELFKVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAIYHVNVVFMGFAIT 84
Cdd:cd21567    1 SNMTLTTEQIIAIIKDWNFAWSIIFLLITIVLQYGYPSRSMTVYVFKMFVLWLLWPSSMALSIFSAVYPIDLASQIISGI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  85 FAIIVGCMWLGYWIASIRLFRRTGSVWSFNPETNRLLNVAIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGRADLES 164
Cdd:cd21567   81 LAAISAMMWISYFVQSIRLFMRTGSWWSFNPETNCLLNVPIGGTTVVRPLVEDSTSVTAVVTNGYLKMAGMHFGACDYDR 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 675803261 165 LPNEITVATSRTLSYFKMSRKVNIGVGSGVATYLRYKVGNHRVPNARASED 215
Cdd:cd21567  161 LPNEVTVAKPNVLIALKMVKRQSYGTNSGVAIYHRYKAGNYRRPPIVADEE 211
HCoV-like_M cd21568
Membrane (or Matrix) protein from human coronavirus and related betacoronaviruses in the A ...
 10-220 3.52e-62

Membrane (or Matrix) protein from human coronavirus and related betacoronaviruses in the A lineage; This group contains the Membrane (M) protein of human coronaviruses (HCoVs), HCoV-OC43 and HCoV-HKU1, and similar proteins from betacoronaviruses in the embecovirus subgenera (A lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394854  Cd Length: 220  Bit Score: 193.47  E-value: 3.52e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  10 TQEELFKVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAIYHVNVVFMGFAITFAIIV 89
Cdd:cd21568    8 TADEAIKFLKEWNFSLGIILLFITIILQFGYTSRSMFVYVIKMIILWLMWPLTIILTIFNCVYALNNVYLGFSIVFTIVS 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  90 GCMWLGYWIASIRLFRRTGSVWSFNPETNRLLNVAIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGRA-DLESLPNE 168
Cdd:cd21568   88 IIMWIVYFVNSIRLFIRTGSWWSFNPETNNLMCIDMKGTMYVRPIIEDYHTLTATIIRGHLYIQGVKLGTGySLSDLPAY 167

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 675803261 169 ITVATSRTLSYFKMSRKVNIGVGSGVATYLRYKVGNHRVPNARASEDQEDLL 220
Cdd:cd21568  168 VTVAKVSHLCTYKRAFLDKIDDTSGFAVYVKSKVGNYRLPSNKGSGMDTALL 219
CoV_M cd21529
coronavirus Membrane (or Matrix) protein; This family contains the Membrane (M) protein of ...
 16-204 1.52e-61

coronavirus Membrane (or Matrix) protein; This family contains the Membrane (M) protein of coronaviruses (CoVs) including three highly pathogenic human CoVs such as Middle East respiratory syndrome (MERS)-related CoV, severe acute respiratory syndrome (SARS) CoV, and SARS-CoV-2, also known as 2019 novel CoV (2019-nCoV) or COVID-19 virus. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394849 [Multi-domain]  Cd Length: 198  Bit Score: 190.79  E-value: 1.52e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  16 KVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAIYHVN--VVFMGFAITFAIIVGCMW 93
Cdd:cd21529    3 SLLEWWNLVLIIILLALIAILQFGYATRSRMIYIIKLIVLWLLWPLTLAVTIWAAVYHGNskDAVFIISIIMAVLTFLMW 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  94 LGYWIASIRLFRRTGSVWSFNPETNRLLNV-AIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGRAD-LESLPNEITV 171
Cdd:cd21529   83 LKYWIDSFRLFMRTRSAWSFNPESNALAGImDPMGQWRCIPIDHMPPVISPVIKHGVLYLHGQELATGVsVHSLPKGITV 162

                        170       180       190
                 ....*....|....*....|....*....|...
gi 675803261 172 ATSRTLSYFKMSRKVNIGVGSGVATYLRYKVGN 204
Cdd:cd21529  163 ATPSTTFIYTLVKRIESTNDRGFAVYIYVKHGA 195
alphaCoV_M cd21564
alphacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) ...
 7-216 2.23e-46

alphacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein of alphacoronaviruses including human coronaviruses (HCoVs), HCoV-229E and HCoV-NL63. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394850  Cd Length: 218  Bit Score: 152.80  E-value: 2.23e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261   7 NTITQEELFKVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAI--YHVNVVFMGFAIT 84
Cdd:cd21564    1 GCIPVGEVIWHLRNWNFSWNIILTIFIVVLQYGHYKYSWFLYGLKMLILWLLWPLVLALSIFDAWasFQVNWVFFAFSIL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  85 FAIIVGCMWLGYWIASIRLFRRTGSVWSFNPETNRLLNVAIRGTMYTRPLQEDTAVIVATVARGVAVFAGHKL-GRADLE 163
Cdd:cd21564   81 MACITLVLWIMYFVNSIRLYRRTHSWWSFNPETNAILCVSVLGRSYCLPVLGAPTGVTLTLLSGTLLVEGFKVaTGVQVD 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 675803261 164 SLPNEITVAT-SRTLSYFKMSRKVNIGVGSGVATYLRYKVGN---HRVPNARASEDQ 216
Cdd:cd21564  161 QLPKYVTVAKpSTTIVYTRVGRSLNASSATGWAFYVRSKHGDysaVANPTDNLSESE 217
gammaCoV_M cd21566
gammacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) ...
 8-185 6.33e-36

gammacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein of gammacoronavirus including avian infectious bronchitis virus (IBV). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394852  Cd Length: 212  Bit Score: 126.01  E-value: 6.33e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261   8 TITQEELFKVVKEWNFALGIMLFIFITLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAIYHVNVVFMGFAITFAI 87
Cdd:cd21566    2 TLDTEQAVELFKEYNLFITAFLLFLTILLQYGYATRSRFIYILKMIVLWCFWPLNIAVGVISCIYPPNTGGLVAAIILTV 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  88 IVGCMWLGYWIASIRLFRRTGSVWSFNPETNRLLNVAI-RGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGRADLESLP 166
Cdd:cd21566   82 FACLSFVGYWIQSIRLFKRCRSWWSFNPESNAVGSILLtNGQQCNFAIESVPMVLSPIIKNGVLYCEGQWLAKCEPDHLP 161

                        170
                 ....*....|....*....
gi 675803261 167 NEITVATSRTLSYFKMSRK 185
Cdd:cd21566  162 KDIFVCTPDRRNIYRMVQK 180
deltaCoV_M cd21594
deltacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) ...
 20-179 1.45e-20

deltacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein of deltacoronaviruses including porcine deltacoronavirus and Bulbul coronavirus HKU11. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394857  Cd Length: 217  Bit Score: 85.96  E-value: 1.45e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  20 EWNfalgIMLFIFI-----TLLQYAYATRSLGLYILKLVLLWLMWPLTFACFVLAAIYHVN----VVFMgFAITFAIIVG 90
Cdd:cd21594    6 EWQ----IIVFIIIiwalgVILQGGYATRNRMIYVIKLILLWLLQPFTLVVTIWTAVDNGSkktdAVFI-ISIIFAVLTF 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 675803261  91 CMWLGYWIASIRLFRRTGSVWSFNPETnRLLNVAI--RGTMYTRPLQEDTAVIVATVARGVAVFAGHKLGRA-DLESLPN 167
Cdd:cd21594   81 VSWLKYWYDSIRLVMKTKSAWSFSPES-RLLAGIMdpMGNWRCIPIDHMAPILTPVVKHGKLKLHGQELATGiSVTSPPK 159

                        170
                 ....*....|...
gi 675803261 168 EITVAT-SRTLSY 179
Cdd:cd21594  160 DMVVVSpSDTFHY 172
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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