NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1449743142|gb|AXN69991|]
View 

triose-phosphate isomerase [Cloning vector pTrc-tpiA]

Protein Classification

triose-phosphate isomerase( domain architecture ID 10791623)

triose-phosphate isomerase (TIM) is a glycolytic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate

EC:  5.3.1.-
Gene Ontology:  GO:0004807|GO:0006094|GO:0006096

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
tpiA PRK00042
triosephosphate isomerase; Provisional
 2-251 3.48e-141

triosephosphate isomerase; Provisional


:

Pssm-ID: 234589  Cd Length: 250  Bit Score: 396.03  E-value: 3.48e-141
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   2 RHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAA 81
Cdd:PRK00042    1 RKPIIAGNWKMNKTLAEAKALVEELKAALPDADGVEVAVAPPFTALASVKEALKGSNIKLGAQNVHPEDSGAFTGEISAE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  82 MLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEG 161
Cdd:PRK00042   81 MLKDLGVKYVIIGHSERRQYFGETDELVNKKVKAALKAGLTPILCVGETLEEREAGKTEEVVARQLEAALAGLSAEQFAN 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 162 AVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADA 241
Cdd:PRK00042  161 LVIAYEPVWAIGTGKTATPEQAQEVHAFIRAVLAELYGEVAEKVRILYGGSVKPDNAAELMAQPDIDGALVGGASLKAED 240

                         250
                  ....*....|
gi 1449743142 242 FAVIVKAAEA 251
Cdd:PRK00042  241 FLAIVKAAAA 250
 
Name Accession Description Interval E-value
tpiA PRK00042
triosephosphate isomerase; Provisional
 2-251 3.48e-141

triosephosphate isomerase; Provisional


Pssm-ID: 234589  Cd Length: 250  Bit Score: 396.03  E-value: 3.48e-141
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   2 RHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAA 81
Cdd:PRK00042    1 RKPIIAGNWKMNKTLAEAKALVEELKAALPDADGVEVAVAPPFTALASVKEALKGSNIKLGAQNVHPEDSGAFTGEISAE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  82 MLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEG 161
Cdd:PRK00042   81 MLKDLGVKYVIIGHSERRQYFGETDELVNKKVKAALKAGLTPILCVGETLEEREAGKTEEVVARQLEAALAGLSAEQFAN 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 162 AVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADA 241
Cdd:PRK00042  161 LVIAYEPVWAIGTGKTATPEQAQEVHAFIRAVLAELYGEVAEKVRILYGGSVKPDNAAELMAQPDIDGALVGGASLKAED 240

                         250
                  ....*....|
gi 1449743142 242 FAVIVKAAEA 251
Cdd:PRK00042  241 FLAIVKAAAA 250
TpiA COG0149
Triosephosphate isomerase [Carbohydrate transport and metabolism]; Triosephosphate isomerase ...
 1-248 1.33e-140

Triosephosphate isomerase [Carbohydrate transport and metabolism]; Triosephosphate isomerase is part of the Pathway/BioSystem: Glycolysis


Pssm-ID: 439919 [Multi-domain]  Cd Length: 249  Bit Score: 394.42  E-value: 1.33e-140
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   1 MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSA 80
Cdd:COG0149     1 MRKPLIAGNWKMNGTLAEAKALLAALAAALADLADVEVVVCPPFTYLAAVAEALAGSPIALGAQNVHWEDSGAYTGEISA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  81 AMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE 160
Cdd:COG0149    81 AMLKDLGCRYVIVGHSERRQYFGETDELVNKKVKAALAAGLTPILCVGETLEEREAGKTEEVVARQLKAALAGLSAEQAA 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 161 GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKV-DANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKA 239
Cdd:COG0149   161 NVVIAYEPVWAIGTGKTATPEQAQEVHAFIRALLAELyGAEVAEAVRILYGGSVKPGNAAELFAQPDIDGALVGGASLDA 240


                  ....*....
gi 1449743142 240 DAFAVIVKA 248
Cdd:COG0149   241 EDFLAIVRA 249
TIM cd00311
Triosephosphate isomerase (TIM) is a glycolytic enzyme that catalyzes the interconversion of ...
 4-247 1.49e-127

Triosephosphate isomerase (TIM) is a glycolytic enzyme that catalyzes the interconversion of dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. The reaction is very efficient and requires neither cofactors nor metal ions. TIM, usually homodimeric, but in some organisms tetrameric, is ubiqitous and conserved in function across eukaryotes, bacteria and archaea.


Pssm-ID: 238190  Cd Length: 242  Bit Score: 361.08  E-value: 1.49e-127
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   4 PLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAML 83
Cdd:cd00311     1 PLVAGNWKMNGTLAEALELAKALNAVLKDESGVEVVVAPPFTYLAAVAEALEGSKIKVGAQNVSPEDSGAFTGEISAEML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  84 KDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKtqGAAAFEGAV 163
Cdd:cd00311    81 KDAGAKYVIIGHSERRQYFGETDEDVAKKVKAALEAGLTPILCVGETLEEREAGKTEEVVAAQLAAVLA--GVEDLAPVV 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 164 IAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADAFA 243
Cdd:cd00311   159 IAYEPVWAIGTGKTASPEQAQEVHAFIRKLLAELYGEVAEKVRILYGGSVNPENAAELLAQPDIDGVLVGGASLKAESFL 238


                  ....
gi 1449743142 244 VIVK 247
Cdd:cd00311   239 DIIK 242
TIM pfam00121
Triosephosphate isomerase; Triosephosphate isomerase (EC:5.3.1.1) (TIM) is the glycolytic ...
 4-249 3.68e-125

Triosephosphate isomerase; Triosephosphate isomerase (EC:5.3.1.1) (TIM) is the glycolytic enzyme that catalyzes the reversible interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. TIM plays an important role in several metabolic pathways and is essential for efficient energy production, present in eukaryotes and prokaryotes. TIM is a dimer of identical subunits, each of which is made up of about 250 amino-acid residues. A glutamic acid residue is involved in the catalytic mechanism. The tertiary structure of TIM has eight beta/alpha motifs folded into a barrel structure. The sequence around the active site residue is perfectly conserved in all known TIM's. Deficiencies in TIM are associated with haemolytic anaemia coupled with a progressive, severe neurological disorder.


Pssm-ID: 459680  Cd Length: 244  Bit Score: 355.28  E-value: 3.68e-125
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   4 PLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEgSHIMLGAQNVDLNLSGAFTGETSAAML 83
Cdd:pfam00121   1 PIIAGNWKMNGTLAEAAELLAELAEALADESGVEVVVAPPFTYLSAVAELLG-SNIKVGAQNVDPEESGAFTGEISAEML 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  84 KDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAfEGAV 163
Cdd:pfam00121  80 KDLGVSYVIIGHSERRQYFGETDEDVAKKVKAALKAGLTPILCVGETLEEREAGKTEEVVARQLDAALAGLGAEQ-KNLV 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 164 IAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADAFA 243
Cdd:pfam00121 159 IAYEPVWAIGTGKTATPEQAQEVHAFIRAVLAELYKEVAEGVRILYGGSVKPGNAAELAAQPDIDGALVGGASLKAEDFL 238


                  ....*.
gi 1449743142 244 VIVKAA 249
Cdd:pfam00121 239 DIINAA 244
tim TIGR00419
triosephosphate isomerase; Triosephosphate isomerase (tim/TPIA) is the glycolytic enzyme that ...
 5-240 3.28e-92

triosephosphate isomerase; Triosephosphate isomerase (tim/TPIA) is the glycolytic enzyme that catalyzes the reversible interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. The active site of the enzyme is located between residues 240-258 of the model ([AV]-Y-E-P-[LIVM]-W-[SA]-I-G-T-[GK]) with E being the active site residue. There is a slight deviation from this sequence within the archeal members of this family. [Energy metabolism, Glycolysis/gluconeogenesis]


Pssm-ID: 129513 [Multi-domain]  Cd Length: 205  Bit Score: 270.52  E-value: 3.28e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   5 LVMGNWK-LNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEgshIMLGAQNVDLNLSGAFTGETSAAML 83
Cdd:TIGR00419   1 LVIGNWKtYNESRGMRALEVAKIAEEVASEAGVAVAVAPPFVDLPMIKREVE---IPVYAQHVDAVLSGAHTGEISAEML 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  84 KDIGAQYIIIGHSERRtyHKESDelIAKKFAVLKEQGLTPVLCIgeteaeneagkteevcarqiDAVLKTQGAAAFEGAV 163
Cdd:TIGR00419  78 KDIGAKGTLINHSERR--MKLAD--IEKKIARLKELGLTSVVCT--------------------NNVLTTAAAAALEPDV 133

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1449743142 164 IAYEPVWAIGTGKSATPAQAQAVHKFIRdhiakVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKAD 240
Cdd:TIGR00419 134 VAVEPPELIGTGIPVSPAQPEVVHGSVR-----AVKEVNESVRVLCGAGISTGEDAELAAQLGAEGVLLASGSLKAD 205
 
Name Accession Description Interval E-value
tpiA PRK00042
triosephosphate isomerase; Provisional
 2-251 3.48e-141

triosephosphate isomerase; Provisional


Pssm-ID: 234589  Cd Length: 250  Bit Score: 396.03  E-value: 3.48e-141
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   2 RHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAA 81
Cdd:PRK00042    1 RKPIIAGNWKMNKTLAEAKALVEELKAALPDADGVEVAVAPPFTALASVKEALKGSNIKLGAQNVHPEDSGAFTGEISAE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  82 MLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEG 161
Cdd:PRK00042   81 MLKDLGVKYVIIGHSERRQYFGETDELVNKKVKAALKAGLTPILCVGETLEEREAGKTEEVVARQLEAALAGLSAEQFAN 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 162 AVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADA 241
Cdd:PRK00042  161 LVIAYEPVWAIGTGKTATPEQAQEVHAFIRAVLAELYGEVAEKVRILYGGSVKPDNAAELMAQPDIDGALVGGASLKAED 240

                         250
                  ....*....|
gi 1449743142 242 FAVIVKAAEA 251
Cdd:PRK00042  241 FLAIVKAAAA 250
TpiA COG0149
Triosephosphate isomerase [Carbohydrate transport and metabolism]; Triosephosphate isomerase ...
 1-248 1.33e-140

Triosephosphate isomerase [Carbohydrate transport and metabolism]; Triosephosphate isomerase is part of the Pathway/BioSystem: Glycolysis


Pssm-ID: 439919 [Multi-domain]  Cd Length: 249  Bit Score: 394.42  E-value: 1.33e-140
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   1 MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSA 80
Cdd:COG0149     1 MRKPLIAGNWKMNGTLAEAKALLAALAAALADLADVEVVVCPPFTYLAAVAEALAGSPIALGAQNVHWEDSGAYTGEISA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  81 AMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE 160
Cdd:COG0149    81 AMLKDLGCRYVIVGHSERRQYFGETDELVNKKVKAALAAGLTPILCVGETLEEREAGKTEEVVARQLKAALAGLSAEQAA 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 161 GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKV-DANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKA 239
Cdd:COG0149   161 NVVIAYEPVWAIGTGKTATPEQAQEVHAFIRALLAELyGAEVAEAVRILYGGSVKPGNAAELFAQPDIDGALVGGASLDA 240


                  ....*....
gi 1449743142 240 DAFAVIVKA 248
Cdd:COG0149   241 EDFLAIVRA 249
TIM cd00311
Triosephosphate isomerase (TIM) is a glycolytic enzyme that catalyzes the interconversion of ...
 4-247 1.49e-127

Triosephosphate isomerase (TIM) is a glycolytic enzyme that catalyzes the interconversion of dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. The reaction is very efficient and requires neither cofactors nor metal ions. TIM, usually homodimeric, but in some organisms tetrameric, is ubiqitous and conserved in function across eukaryotes, bacteria and archaea.


Pssm-ID: 238190  Cd Length: 242  Bit Score: 361.08  E-value: 1.49e-127
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   4 PLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAML 83
Cdd:cd00311     1 PLVAGNWKMNGTLAEALELAKALNAVLKDESGVEVVVAPPFTYLAAVAEALEGSKIKVGAQNVSPEDSGAFTGEISAEML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  84 KDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKtqGAAAFEGAV 163
Cdd:cd00311    81 KDAGAKYVIIGHSERRQYFGETDEDVAKKVKAALEAGLTPILCVGETLEEREAGKTEEVVAAQLAAVLA--GVEDLAPVV 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 164 IAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADAFA 243
Cdd:cd00311   159 IAYEPVWAIGTGKTASPEQAQEVHAFIRKLLAELYGEVAEKVRILYGGSVNPENAAELLAQPDIDGVLVGGASLKAESFL 238


                  ....
gi 1449743142 244 VIVK 247
Cdd:cd00311   239 DIIK 242
TIM pfam00121
Triosephosphate isomerase; Triosephosphate isomerase (EC:5.3.1.1) (TIM) is the glycolytic ...
 4-249 3.68e-125

Triosephosphate isomerase; Triosephosphate isomerase (EC:5.3.1.1) (TIM) is the glycolytic enzyme that catalyzes the reversible interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. TIM plays an important role in several metabolic pathways and is essential for efficient energy production, present in eukaryotes and prokaryotes. TIM is a dimer of identical subunits, each of which is made up of about 250 amino-acid residues. A glutamic acid residue is involved in the catalytic mechanism. The tertiary structure of TIM has eight beta/alpha motifs folded into a barrel structure. The sequence around the active site residue is perfectly conserved in all known TIM's. Deficiencies in TIM are associated with haemolytic anaemia coupled with a progressive, severe neurological disorder.


Pssm-ID: 459680  Cd Length: 244  Bit Score: 355.28  E-value: 3.68e-125
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   4 PLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEgSHIMLGAQNVDLNLSGAFTGETSAAML 83
Cdd:pfam00121   1 PIIAGNWKMNGTLAEAAELLAELAEALADESGVEVVVAPPFTYLSAVAELLG-SNIKVGAQNVDPEESGAFTGEISAEML 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  84 KDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAfEGAV 163
Cdd:pfam00121  80 KDLGVSYVIIGHSERRQYFGETDEDVAKKVKAALKAGLTPILCVGETLEEREAGKTEEVVARQLDAALAGLGAEQ-KNLV 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 164 IAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADAFA 243
Cdd:pfam00121 159 IAYEPVWAIGTGKTATPEQAQEVHAFIRAVLAELYKEVAEGVRILYGGSVKPGNAAELAAQPDIDGALVGGASLKAEDFL 238


                  ....*.
gi 1449743142 244 VIVKAA 249
Cdd:pfam00121 239 DIINAA 244
PTZ00333 PTZ00333
triosephosphate isomerase; Provisional
 1-253 8.33e-101

triosephosphate isomerase; Provisional


Pssm-ID: 240365  Cd Length: 255  Bit Score: 293.74  E-value: 8.33e-101
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   1 MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSA 80
Cdd:PTZ00333    3 KRKPFVGGNWKCNGTKASIKELIDSFNKLKFDPNNVDVVVAPPSLHIPLVQEKLKNKNFKISSQNVSLTGSGAFTGEISA 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  81 AMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE 160
Cdd:PTZ00333   83 EMLKDLGINWTILGHSERRQYFGETNEIVAQKVKNALENGLKVILCIGETLEEREAGQTSDVLSKQLEAIVKKVSDEAWD 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 161 GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKA 239
Cdd:PTZ00333  163 NIVIAYEPVWAIGTGKVATPEQAQEVHAFIRKWLAeKVGADVAEATRIIYGGSVNEKNCKELIKQPDIDGFLVGGASLKP 242

                         250
                  ....*....|....
gi 1449743142 240 DaFAVIVKAAEAAK 253
Cdd:PTZ00333  243 D-FVDIIKSAEQSK 255
tim TIGR00419
triosephosphate isomerase; Triosephosphate isomerase (tim/TPIA) is the glycolytic enzyme that ...
 5-240 3.28e-92

triosephosphate isomerase; Triosephosphate isomerase (tim/TPIA) is the glycolytic enzyme that catalyzes the reversible interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. The active site of the enzyme is located between residues 240-258 of the model ([AV]-Y-E-P-[LIVM]-W-[SA]-I-G-T-[GK]) with E being the active site residue. There is a slight deviation from this sequence within the archeal members of this family. [Energy metabolism, Glycolysis/gluconeogenesis]


Pssm-ID: 129513 [Multi-domain]  Cd Length: 205  Bit Score: 270.52  E-value: 3.28e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   5 LVMGNWK-LNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEgshIMLGAQNVDLNLSGAFTGETSAAML 83
Cdd:TIGR00419   1 LVIGNWKtYNESRGMRALEVAKIAEEVASEAGVAVAVAPPFVDLPMIKREVE---IPVYAQHVDAVLSGAHTGEISAEML 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  84 KDIGAQYIIIGHSERRtyHKESDelIAKKFAVLKEQGLTPVLCIgeteaeneagkteevcarqiDAVLKTQGAAAFEGAV 163
Cdd:TIGR00419  78 KDIGAKGTLINHSERR--MKLAD--IEKKIARLKELGLTSVVCT--------------------NNVLTTAAAAALEPDV 133

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1449743142 164 IAYEPVWAIGTGKSATPAQAQAVHKFIRdhiakVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKAD 240
Cdd:TIGR00419 134 VAVEPPELIGTGIPVSPAQPEVVHGSVR-----AVKEVNESVRVLCGAGISTGEDAELAAQLGAEGVLLASGSLKAD 205
PRK13962 PRK13962
bifunctional phosphoglycerate kinase/triosephosphate isomerase; Provisional
 1-248 1.61e-89

bifunctional phosphoglycerate kinase/triosephosphate isomerase; Provisional


Pssm-ID: 237572 [Multi-domain]  Cd Length: 645  Bit Score: 277.76  E-value: 1.61e-89
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   1 MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGcAVAIAPPEMYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSA 80
Cdd:PRK13962  396 PRKPIIAGNWKMNKTPAEAKEFVNELKKYVKDAQA-EVVVCPPFTALPSVKEAVDGSNIKLGAQNVFYEEKGAYTGEISG 474

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  81 AMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE 160
Cdd:PRK13962  475 PMLAEIGVEYVIIGHSERRQYFGETDELVNKKVLAALKAGLTPILCVGETLDERESGITFDVVRLQLKAALNGLSAEQVK 554

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 161 GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKA 239
Cdd:PRK13962  555 KVVIAYEPVWAIGTGKVATPEQAQEVHAFIRKLVAeLYGEEAARKVRILYGGSVKSENAAGLFNQPDIDGGLVGGASLKA 634


                  ....*....
gi 1449743142 240 DAFAVIVKA 248
Cdd:PRK13962  635 QEFAAIANY 643
PLN02561 PLN02561
triosephosphate isomerase
 2-254 1.93e-67

triosephosphate isomerase


Pssm-ID: 178175  Cd Length: 253  Bit Score: 209.29  E-value: 1.93e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   2 RHPLVMGNWKLNGSRHMVHELVSNLRK-ELAGVAGCAVAIAPPEMYIDMAKREAEgSHIMLGAQNVDLNLSGAFTGETSA 80
Cdd:PLN02561    3 RKFFVGGNWKCNGTVEEVKKIVTTLNEaEVPSEDVVEVVVSPPFVFLPLVKSLLR-PDFQVAAQNCWVKKGGAFTGEISA 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  81 AMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVlkTQGAAAFE 160
Cdd:PLN02561   82 EMLVNLGIPWVILGHSERRALLGESNEFVGDKVAYALSQGLKVIACVGETLEQRESGSTMDVVAAQTKAI--ADKVSDWA 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 161 GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAK-VDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKA 239
Cdd:PLN02561  160 NVVLAYEPVWAIGTGKVATPAQAQEVHDELRKWLHKnVSPEVAATTRIIYGGSVTGANCKELAAQPDVDGFLVGGASLKP 239

                         250
                  ....*....|....*
gi 1449743142 240 DaFAVIVKAAEAAKQ 254
Cdd:PLN02561  240 E-FIDIIKSATVKKS 253
PRK14565 PRK14565
triosephosphate isomerase; Provisional
 5-250 1.68e-57

triosephosphate isomerase; Provisional


Pssm-ID: 237758  Cd Length: 237  Bit Score: 183.04  E-value: 1.68e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   5 LVMGNWKLNGSRHMVHELVSNLRKELAGV-AGCAVAIAPPemYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAML 83
Cdd:PRK14565    4 LIVANWKMNGDFSLFSSFLKELSNKLANNeITLKLVICPP--FTAMSSFVECNPNIKLGAQNCFYGSSGGYTGEISAKML 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  84 KDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAaafegAV 163
Cdd:PRK14565   82 KECGCSYVILGHSERRSTFHETDSDIRLKAESAIESGLIPIICVGETLEDRENGMTKDVLLEQCSNCLPKHGE-----FI 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 164 IAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDaniaeqviIQYGGSVNASNAAELFAQPDIDGALVGGASLKADAFA 243
Cdd:PRK14565  157 IAYEPVWAIGGSTIPSNDAIAEAFEIIRSYDSKSH--------IIYGGSVNQENIRDLKSINQLSGVLVGSASLDVDSFC 228


                  ....*..
gi 1449743142 244 VIVKAAE 250
Cdd:PRK14565  229 KIIQQVE 235
PLN02429 PLN02429
triosephosphate isomerase
 13-255 3.51e-56

triosephosphate isomerase


Pssm-ID: 166070  Cd Length: 315  Bit Score: 182.30  E-value: 3.51e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  13 NGSRHMVHELVSNLRKELAGvAGCAVAIAPPEMYIDMAKREAEgSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYII 92
Cdd:PLN02429   75 NGTKDSIAKLISDLNSATLE-ADVDVVVSPPFVYIDQVKSSLT-DRIDISGQNSWVGKGGAFTGEISVEQLKDLGCKWVI 152

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  93 IGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVlkTQGAAAFEGAVIAYEPVWAI 172
Cdd:PLN02429  153 LGHSERRHVIGEKDEFIGKKAAYALSEGLGVIACIGEKLEEREAGKTFDVCFAQLKAF--ADAVPSWDNIVVAYEPVWAI 230

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 173 GTGKSATPAQAQAVHKFIRDHIAK-VDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADAFAVIVKAAEA 251
Cdd:PLN02429  231 GTGKVASPQQAQEVHVAVRGWLKKnVSEEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPEFATIVNSVTS 310


                  ....
gi 1449743142 252 AKQA 255
Cdd:PLN02429  311 KKVA 314
PRK15492 PRK15492
triosephosphate isomerase; Provisional
 59-247 2.25e-42

triosephosphate isomerase; Provisional


Pssm-ID: 185389  Cd Length: 260  Bit Score: 145.13  E-value: 2.25e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  59 IMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYHKESD-ELIAKKFAVLKEQgLTPVLCIGETEAENEAG 137
Cdd:PRK15492   66 IIIGAQNMNPNDNGQFTGDISPLMLKEIGTQLVMIGHSERRHKFGETDqEENAKVLAALKHD-FTTLLCVGETLEQKNYG 144

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 138 KTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGT-GKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNAS 216
Cdd:PRK15492  145 ISDEILRTQLKIGLHGINPDQLAKLRIAYEPVWAIGEaGIPASADYADEKHAVIKQCLIELFGDAGDDIPVFYGGSVNAE 224

                         170       180       190
                  ....*....|....*....|....*....|.
gi 1449743142 217 NAAELFAQPDIDGALVGGASLKADAFAVIVK 247
Cdd:PRK15492  225 NANELFGQPHIDGLFIGRSAWDADKFFAIIE 255
PRK14905 PRK14905
triosephosphate isomerase/PTS system glucose/sucrose-specific transporter subunit IIB; ...
 47-249 4.01e-40

triosephosphate isomerase/PTS system glucose/sucrose-specific transporter subunit IIB; Provisional


Pssm-ID: 184898 [Multi-domain]  Cd Length: 355  Bit Score: 141.71  E-value: 4.01e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  47 IDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLC 126
Cdd:PRK14905   55 VEAAASETGHPKIKIGAQNMNAKDKGQFTGEISPLMLKELGIELVMIGHSERRHVLKETDQEENEKVLAALKHGFITLLC 134

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 127 IGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGksATPAQAQAV---HKFIRDHIAKVDANIAE 203
Cdd:PRK14905  135 IGETLEQKNYNISDEVLRTQLKIGLHGVSAEQLPHLFIAYEPVWAIGEG--GIPASAEYAdekHAIIKQCLFELFAEESK 212

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1449743142 204 QVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADAFAVIVKAA 249
Cdd:PRK14905  213 KIPVLYGGSVNLENANELIMKPHIDGLFIGRSAWDAQCFHALIADA 258
PRK04302 PRK04302
triosephosphate isomerase; Provisional
 38-240 6.01e-10

triosephosphate isomerase; Provisional


Pssm-ID: 235274  Cd Length: 223  Bit Score: 57.57  E-value: 6.01e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  38 VAIAPPEMYIDMAKREAEgshIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYHKESDELIAKkfavLK 117
Cdd:PRK04302   39 IAVAPQALDIRRVAEEVD---IPVYAQHVDPVEPGSHTGHILPEAVKDAGAVGTLINHSERRLTLADIEAVVER----AK 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142 118 EQGLTPVLCigeteAENeagkteevcarqidaVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIrDHIAKV 197
Cdd:PRK04302  112 KLGLESVVC-----VNN---------------PETSAAAAALGPDYVAVEPPELIGTGIPVSKAKPEVVEDAV-EAVKKV 170

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1449743142 198 DANIAeqVI----IQYGGSVNAsnAAELFAqpdiDGALVGGASLKAD 240
Cdd:PRK04302  171 NPDVK--VLcgagISTGEDVKA--ALELGA----DGVLLASGVVKAK 209
TIM_phosphate_binding cd04722
TIM barrel proteins share a structurally conserved phosphate binding motif and in general ...
 6-234 5.74e-06

TIM barrel proteins share a structurally conserved phosphate binding motif and in general share an eight beta/alpha closed barrel structure. Specific for this family is the conserved phosphate binding site at the edges of strands 7 and 8. The phosphate comes either from the substrate, as in the case of inosine monophosphate dehydrogenase (IMPDH), or from ribulose-5-phosphate 3-epimerase (RPE) or from cofactors, like FMN.


Pssm-ID: 240073 [Multi-domain]  Cd Length: 200  Bit Score: 45.66  E-value: 5.74e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142   6 VMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYI----DMAKREAEGSHIMLGAQNVDLNLSGAFTGEtsAA 81
Cdd:cd04722     1 VILALLAGGPSGDPVELAKAAAEAGADAIIVGTRSSDPEEAEtddkEVLKEVAAETDLPLGVQLAINDAAAAVDIA--AA 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1449743142  82 MLKDIGAQYIIIGHSERRTyhkesDELIAKKFAVLKEQ--GLTPVLCIGETEAENEAGkteevcarqidavlktqgAAAF 159
Cdd:cd04722    79 AARAAGADGVEIHGAVGYL-----AREDLELIRELREAvpDVKVVVKLSPTGELAAAA------------------AEEA 135

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1449743142 160 EGAVIAYEPVWAIGTGKSATPaqaqavhkfiRDHIAKVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGG 234
Cdd:cd04722   136 GVDEVGLGNGGGGGGGRDAVP----------IADLLLILAKRGSKVPVIAGGGINDPEDAAEALALGADGVIVGS 200
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH