OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved cysteine and histidine, and in most cases an aspartate, as the catalytic triad. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1827633752 691 KAIDVPGDGKCGLHAVVQDLQSHGLLMQRdggrvlRLLDKSTNAPTFHSP-------------EEIAGAVLKLGLGLDV- 756
Cdd:cd22792     1 KVVPVPGDGNCFWHSLGHFLGLSALELKK------LLRDSLFDDPELDEEldeqlepgvyaedEAIAAAAKLFGVNICVh 74

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1827633752 757 -VADGIVHSY-GNNDGHRILLQLKDHHYMPIV 786
Cdd:cd22792    75 dPDEGVLYTFtPNESSKSIHLLLENEHFEPLV 106

OTU (ovarian tumor) domain of the potexviruses/carlaviruses RNA replication protein family; RNA replication polyprotein (RDRP) is a viral homolog of ovarian tumor protease (vOTU), which displays RNA helicase (EC 3.6.4.13), RNA-directed RNA polymerase (EC 2.7.7.48), viral methyltransferase, Fe(2+) 2-oxoglutarate dioxygenase and protease activities. The central part of this protein possibly functions as an ATP-binding helicase. It is an RNA-directed RNA polymerase involved in viral RNA replication. It also acts as a thiol protease that cleaves the polyprotein. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1827633752 691 KAIDVPGDGKCGLHAVVQDLQSHGLLMQRdggrvlRLLDKSTNAPTFHSP-------------EEIAGAVLKLGLGLDV- 756
Cdd:cd22792     1 KVVPVPGDGNCFWHSLGHFLGLSALELKK------LLRDSLFDDPELDEEldeqlepgvyaedEAIAAAAKLFGVNICVh 74

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1827633752 757 -VADGIVHSY-GNNDGHRILLQLKDHHYMPIV 786
Cdd:cd22792    75 dPDEGVLYTFtPNESSKSIHLLLENEHFEPLV 106

OTU (ovarian tumor) domain of the potexviruses/carlaviruses RNA replication protein family; RNA replication polyprotein (RDRP) is a viral homolog of ovarian tumor protease (vOTU), which displays RNA helicase (EC 3.6.4.13), RNA-directed RNA polymerase (EC 2.7.7.48), viral methyltransferase, Fe(2+) 2-oxoglutarate dioxygenase and protease activities. The central part of this protein possibly functions as an ATP-binding helicase. It is an RNA-directed RNA polymerase involved in viral RNA replication. It also acts as a thiol protease that cleaves the polyprotein. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1827633752 691 KAIDVPGDGKCGLHAVVQDLQSHGLLMQRdggrvlRLLDKSTNAPTFHSP-------------EEIAGAVLKLGLGLDV- 756
Cdd:cd22792     1 KVVPVPGDGNCFWHSLGHFLGLSALELKK------LLRDSLFDDPELDEEldeqlepgvyaedEAIAAAAKLFGVNICVh 74

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1827633752 757 -VADGIVHSY-GNNDGHRILLQLKDHHYMPIV 786
Cdd:cd22792    75 dPDEGVLYTFtPNESSKSIHLLLENEHFEPLV 106