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Conserved domains on  [gi|119591353|gb|EAW70947|]
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proteasome (prosome, macropain) 26S subunit, non-ATPase, 1, isoform CRA_a [Homo sapiens]

Protein Classification

26S proteasome non-ATPase regulatory subunit 1( domain architecture ID 1001948)

26S proteasome non-ATPase regulatory subunit 1 (PSMD1), also known as RPN2, is a non-ATPase subunit of the 19S regulatory complex of the 26S proteasome which plays a critical role in the recognition of ubiquitinated protein substrates by the proteasome and is involved in the ATP-dependent unfolding and translocation of these substrates into the proteasome core for degradation

Gene Ontology:  GO:0043161|GO:0005838

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
RPN2 super family cl34911
26S proteasome regulatory complex component [Posttranslational modification, protein turnover, ...
 2-934 0e+00

26S proteasome regulatory complex component [Posttranslational modification, protein turnover, chaperones];


The actual alignment was detected with superfamily member COG5116:

Pssm-ID: 227446 [Multi-domain]  Cd Length: 926  Bit Score: 852.32  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353   2 ITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYHLGAFEESLNYA 81
Cdd:COG5116    3 MTTARILPALLAELRDGRESEALDVINAHVDQLWPEISDDLRYIEALYDDDSFDPREMAALCLSKVYYVLGEYQQAIEYA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353  82 LGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVEnADLpEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLD 161
Cdd:COG5116   83 LRAGDRFLVDDGSFYYETIVYKSIEMYVHMMDS-AYI-GGDKDIIDRILDFVLEVIGAKCVDDSEIGYLLGIAAEGLRLD 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 162 VFEKTILESNDVpGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDN 241
Cdd:COG5116  161 IIEKYLSDGNDC-DIINYLLDLAITLVEEEGFRKEILRMLAEIGPGKPKPDYFYVIKAVVYLNDAEKAKALIEKLVKEND 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 242 LLMAYQICFDLYESASQQFLSSVIqnlrtvgtpiasvpgstnTGTVPGSEKDsdsmeteektssafvgktpeaspepkdq 321
Cdd:COG5116  240 LLLYAQVAFDLEDSASQEILEILV------------------TELVAQGYDQ---------------------------- 273

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 322 tlKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAV--RNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNW 399
Cdd:COG5116  274 --AVMSILSGEFTKKYLGAFLLEKNNTDFKFLNSSKSSLarKFSRFHYAVSLANSFMNLGTSNDSFYRNNLDWLGKASNW 351

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 400 AKFTATASLGVIHKGHEKEALQLMATYLPKDTSpGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASN---DIVRHG 476
Cdd:COG5116  352 AKFTATASLGVIHLGNSNPGYEILKPYLPSEVA-SSRQKEGGALYALGLIKAGFGREDTEYLLEYFLDTEDeltPELAYG 430

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 477 GSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVM 556
Cdd:COG5116  431 VCLGIGLINMGSANREIYEKLKELLKNDRALLGEAAVYGMGLLMLGTWSVEAIEDMRTYAGETQHERIKRGLGIGFALIL 510

                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 557 YGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTPEQCPSV 636
Cdd:COG5116  511 YGRQEMADDYINELLYDKDSILRYNGVFSLALAYVGTGNLGVVSTLLHYAVSDGNDDVRRAAVIALGFVCCDDRDLLVGT 590

                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 637 VSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKV 716
Cdd:COG5116  591 VELLSESHNFHVRAGVAVALGIACAGTGDKVATDILEALMYDTNDFVRQSAMIAVGMILMQCNPELNPNVKRIIKKFNRV 670

                        730       740       750       760       770       780       790       800
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 717 INDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLK 796
Cdd:COG5116  671 IVDKHESGLAKLGAVLAQGISEAGGRNVTISLRNATGILSADRIVGLVLFLQYWYWFPLIHFVSLSFLPTTVIGIRGSQA 750

                        810       820       830       840       850       860       870       880
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 797 MPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITA-------------------KAKKKEKEKEKKEEEKMEVD 857
Cdd:COG5116  751 IPKFCFNEDLEEEEFEYPRMYEEASGKSVRKVNTAVLSTTIkaaarakqkpkekgpndkeIKIESPSVETEGERCTIKQR 830

                        890       900       910       920       930       940       950       960
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 858 EAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSiGGIIILKD-TSEDIEELVE------PVAA 930
Cdd:COG5116  831 EEKGIDAPAILNVKKKKPYKVDNMTRILPQQSRYISFIKDDRFVPVRKFK-GGVVVLRDrEPKEPVALIEtvrqmkDVNA 909


                 ....
gi 119591353 931 HGPK 934
Cdd:COG5116  910 PLPT 913
 
Name Accession Description Interval E-value
RPN2 COG5116
26S proteasome regulatory complex component [Posttranslational modification, protein turnover, ...
 2-934 0e+00

26S proteasome regulatory complex component [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 227446 [Multi-domain]  Cd Length: 926  Bit Score: 852.32  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353   2 ITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYHLGAFEESLNYA 81
Cdd:COG5116    3 MTTARILPALLAELRDGRESEALDVINAHVDQLWPEISDDLRYIEALYDDDSFDPREMAALCLSKVYYVLGEYQQAIEYA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353  82 LGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVEnADLpEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLD 161
Cdd:COG5116   83 LRAGDRFLVDDGSFYYETIVYKSIEMYVHMMDS-AYI-GGDKDIIDRILDFVLEVIGAKCVDDSEIGYLLGIAAEGLRLD 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 162 VFEKTILESNDVpGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDN 241
Cdd:COG5116  161 IIEKYLSDGNDC-DIINYLLDLAITLVEEEGFRKEILRMLAEIGPGKPKPDYFYVIKAVVYLNDAEKAKALIEKLVKEND 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 242 LLMAYQICFDLYESASQQFLSSVIqnlrtvgtpiasvpgstnTGTVPGSEKDsdsmeteektssafvgktpeaspepkdq 321
Cdd:COG5116  240 LLLYAQVAFDLEDSASQEILEILV------------------TELVAQGYDQ---------------------------- 273

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 322 tlKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAV--RNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNW 399
Cdd:COG5116  274 --AVMSILSGEFTKKYLGAFLLEKNNTDFKFLNSSKSSLarKFSRFHYAVSLANSFMNLGTSNDSFYRNNLDWLGKASNW 351

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 400 AKFTATASLGVIHKGHEKEALQLMATYLPKDTSpGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASN---DIVRHG 476
Cdd:COG5116  352 AKFTATASLGVIHLGNSNPGYEILKPYLPSEVA-SSRQKEGGALYALGLIKAGFGREDTEYLLEYFLDTEDeltPELAYG 430

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 477 GSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVM 556
Cdd:COG5116  431 VCLGIGLINMGSANREIYEKLKELLKNDRALLGEAAVYGMGLLMLGTWSVEAIEDMRTYAGETQHERIKRGLGIGFALIL 510

                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 557 YGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTPEQCPSV 636
Cdd:COG5116  511 YGRQEMADDYINELLYDKDSILRYNGVFSLALAYVGTGNLGVVSTLLHYAVSDGNDDVRRAAVIALGFVCCDDRDLLVGT 590

                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 637 VSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKV 716
Cdd:COG5116  591 VELLSESHNFHVRAGVAVALGIACAGTGDKVATDILEALMYDTNDFVRQSAMIAVGMILMQCNPELNPNVKRIIKKFNRV 670

                        730       740       750       760       770       780       790       800
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 717 INDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLK 796
Cdd:COG5116  671 IVDKHESGLAKLGAVLAQGISEAGGRNVTISLRNATGILSADRIVGLVLFLQYWYWFPLIHFVSLSFLPTTVIGIRGSQA 750

                        810       820       830       840       850       860       870       880
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 797 MPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITA-------------------KAKKKEKEKEKKEEEKMEVD 857
Cdd:COG5116  751 IPKFCFNEDLEEEEFEYPRMYEEASGKSVRKVNTAVLSTTIkaaarakqkpkekgpndkeIKIESPSVETEGERCTIKQR 830

                        890       900       910       920       930       940       950       960
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 858 EAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSiGGIIILKD-TSEDIEELVE------PVAA 930
Cdd:COG5116  831 EEKGIDAPAILNVKKKKPYKVDNMTRILPQQSRYISFIKDDRFVPVRKFK-GGVVVLRDrEPKEPVALIEtvrqmkDVNA 909


                 ....
gi 119591353 931 HGPK 934
Cdd:COG5116  910 PLPT 913
RPN2_C pfam18004
26S proteasome regulatory subunit RPN2 C-terminal domain; This is the C-terminal domain found ...
 786-925 1.34e-36

26S proteasome regulatory subunit RPN2 C-terminal domain; This is the C-terminal domain found in S. cerevisiae Rpn2 (26S proteasome regulatory subunit RPN2) as well as other eukaryotic species. A study revealed that the C-terminal 52 residues of the Rpn2 C-terminal domain are responsible for mediating interactions with the ubiquitin-binding subunit Rpn13. Futhermore, the extreme C-terminal 20 or 21 residues of Rpn2 (926-945 or 925-945) of S. cerevisiae, were shown to be equally effective at binding Rpn13. Multiple sequence alignments indicate that Rpn2 orthologs are highly conserved in this C-terminal region and share characteriztic acidic, aromatic, and proline residues, suggesting a common function. In the structure of Rpn2 from S. cerevisiae, this region is exposed and disordered, and is thus accessible for associating with Rpn13. The Rpn2 binding surface of human Rpn13 has been mapped by nuclear magnetic resonance titration to one surface of its Pru domain.


Pssm-ID: 465613 [Multi-domain]  Cd Length: 159  Bit Score: 135.39  E-value: 1.34e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353  786 TCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITA-----KAKKKEKEKEKKEEEKMEVDEAE 860
Cdd:pfam18004   1 TAIIGLNKDLKMPKFEFNSNAKPSLFAYPPETEEKKEKAPEKVATAVLSTTAkakarAKKKEKEKEKEKEEDKMDVDEEK 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 119591353  861 KKEEKEKKKEPEP-------------NFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGGIIILKDT-SEDIEELV 925
Cdd:pfam18004  81 EEKEKKEKEKEEKekkaekkkkekepTFYKLENPSRVLPAQLKYISFIKDSRYVPVKKKGTGGIIVLKDTkPDEPEELI 159
 
Name Accession Description Interval E-value
RPN2 COG5116
26S proteasome regulatory complex component [Posttranslational modification, protein turnover, ...
 2-934 0e+00

26S proteasome regulatory complex component [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 227446 [Multi-domain]  Cd Length: 926  Bit Score: 852.32  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353   2 ITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYHLGAFEESLNYA 81
Cdd:COG5116    3 MTTARILPALLAELRDGRESEALDVINAHVDQLWPEISDDLRYIEALYDDDSFDPREMAALCLSKVYYVLGEYQQAIEYA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353  82 LGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVEnADLpEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLD 161
Cdd:COG5116   83 LRAGDRFLVDDGSFYYETIVYKSIEMYVHMMDS-AYI-GGDKDIIDRILDFVLEVIGAKCVDDSEIGYLLGIAAEGLRLD 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 162 VFEKTILESNDVpGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDN 241
Cdd:COG5116  161 IIEKYLSDGNDC-DIINYLLDLAITLVEEEGFRKEILRMLAEIGPGKPKPDYFYVIKAVVYLNDAEKAKALIEKLVKEND 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 242 LLMAYQICFDLYESASQQFLSSVIqnlrtvgtpiasvpgstnTGTVPGSEKDsdsmeteektssafvgktpeaspepkdq 321
Cdd:COG5116  240 LLLYAQVAFDLEDSASQEILEILV------------------TELVAQGYDQ---------------------------- 273

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 322 tlKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAV--RNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNW 399
Cdd:COG5116  274 --AVMSILSGEFTKKYLGAFLLEKNNTDFKFLNSSKSSLarKFSRFHYAVSLANSFMNLGTSNDSFYRNNLDWLGKASNW 351

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 400 AKFTATASLGVIHKGHEKEALQLMATYLPKDTSpGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASN---DIVRHG 476
Cdd:COG5116  352 AKFTATASLGVIHLGNSNPGYEILKPYLPSEVA-SSRQKEGGALYALGLIKAGFGREDTEYLLEYFLDTEDeltPELAYG 430

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 477 GSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVM 556
Cdd:COG5116  431 VCLGIGLINMGSANREIYEKLKELLKNDRALLGEAAVYGMGLLMLGTWSVEAIEDMRTYAGETQHERIKRGLGIGFALIL 510

                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 557 YGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTPEQCPSV 636
Cdd:COG5116  511 YGRQEMADDYINELLYDKDSILRYNGVFSLALAYVGTGNLGVVSTLLHYAVSDGNDDVRRAAVIALGFVCCDDRDLLVGT 590

                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 637 VSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKV 716
Cdd:COG5116  591 VELLSESHNFHVRAGVAVALGIACAGTGDKVATDILEALMYDTNDFVRQSAMIAVGMILMQCNPELNPNVKRIIKKFNRV 670

                        730       740       750       760       770       780       790       800
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 717 INDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLK 796
Cdd:COG5116  671 IVDKHESGLAKLGAVLAQGISEAGGRNVTISLRNATGILSADRIVGLVLFLQYWYWFPLIHFVSLSFLPTTVIGIRGSQA 750

                        810       820       830       840       850       860       870       880
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 797 MPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITA-------------------KAKKKEKEKEKKEEEKMEVD 857
Cdd:COG5116  751 IPKFCFNEDLEEEEFEYPRMYEEASGKSVRKVNTAVLSTTIkaaarakqkpkekgpndkeIKIESPSVETEGERCTIKQR 830

                        890       900       910       920       930       940       950       960
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 858 EAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSiGGIIILKD-TSEDIEELVE------PVAA 930
Cdd:COG5116  831 EEKGIDAPAILNVKKKKPYKVDNMTRILPQQSRYISFIKDDRFVPVRKFK-GGVVVLRDrEPKEPVALIEtvrqmkDVNA 909


                 ....
gi 119591353 931 HGPK 934
Cdd:COG5116  910 PLPT 913
RPN2_C pfam18004
26S proteasome regulatory subunit RPN2 C-terminal domain; This is the C-terminal domain found ...
 786-925 1.34e-36

26S proteasome regulatory subunit RPN2 C-terminal domain; This is the C-terminal domain found in S. cerevisiae Rpn2 (26S proteasome regulatory subunit RPN2) as well as other eukaryotic species. A study revealed that the C-terminal 52 residues of the Rpn2 C-terminal domain are responsible for mediating interactions with the ubiquitin-binding subunit Rpn13. Futhermore, the extreme C-terminal 20 or 21 residues of Rpn2 (926-945 or 925-945) of S. cerevisiae, were shown to be equally effective at binding Rpn13. Multiple sequence alignments indicate that Rpn2 orthologs are highly conserved in this C-terminal region and share characteriztic acidic, aromatic, and proline residues, suggesting a common function. In the structure of Rpn2 from S. cerevisiae, this region is exposed and disordered, and is thus accessible for associating with Rpn13. The Rpn2 binding surface of human Rpn13 has been mapped by nuclear magnetic resonance titration to one surface of its Pru domain.


Pssm-ID: 465613 [Multi-domain]  Cd Length: 159  Bit Score: 135.39  E-value: 1.34e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353  786 TCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITA-----KAKKKEKEKEKKEEEKMEVDEAE 860
Cdd:pfam18004   1 TAIIGLNKDLKMPKFEFNSNAKPSLFAYPPETEEKKEKAPEKVATAVLSTTAkakarAKKKEKEKEKEKEEDKMDVDEEK 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 119591353  861 KKEEKEKKKEPEP-------------NFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGGIIILKDT-SEDIEELV 925
Cdd:pfam18004  81 EEKEKKEKEKEEKekkaekkkkekepTFYKLENPSRVLPAQLKYISFIKDSRYVPVKKKGTGGIIVLKDTkPDEPEELI 159
RPN1 COG5110
26S proteasome regulatory complex component [Posttranslational modification, protein turnover, ...
 373-800 1.52e-25

26S proteasome regulatory complex component [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 227441 [Multi-domain]  Cd Length: 881  Bit Score: 113.87  E-value: 1.52e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 373 NSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEALQLMATYLPKDTSpgsaYQEGGGLYALGLIH-A 451
Cdd:COG5110  368 NDPINLGYENDSLIPLDDEWIYKCKVPGLISAFASIGVIESWNSDKGLETLDKYLYADES----YRKAGALLGIGLSGlR 443

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 452 NHG-GDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEA--AGLALGLVMLGSKNAQA 528
Cdd:COG5110  444 VFEeRPPALALLSNYLQSSSSKHVIAAILGLGAAFSGTQAEEVLELLQPIMFSTDSPIEVVffASLTLGSVFVGTCNGDL 523

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 529 IEDMV-------GYAQETQhekILRGLAVGIALVMYGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRR 601
Cdd:COG5110  524 TSLILqtfvergKIESETQ---WFRFLALGLASLFYGRKDQVDDVEETIMAIEGALSKHEEILVKGCQYVGTGDVLVIQS 600

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 602 LLHVAVSDVNDDVRR--AAVES---LGFILFRTPEQCPSVVSLLSESY-----NPHVRYGAAMALGICCAGTGNKEAINL 671
Cdd:COG5110  601 LLHVKDEFTGDTLKNeeALIESlalLGCALIAMGEDIGSEMVLRHFSHsmhygSSHIRSVLPLAYGILSPSNPQMNVFDT 680

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 672 LEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVnqFRQLYSKVindkHDDVMAKFGAILAQGILDAGGHNVTISLQSR 751
Cdd:COG5110  681 LERSSHDGDLNVIINTIFAMGLIGAGTLNARLAQL--LRQLASYY----YKESKALFVLRIAQGLLSLGKGTMTISPLYF 754

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 119591353 752 TGHTHMPSVVGVLVFTQFWFW----FPL--SH----FLSLAYTPTCVIGLNKDLKMPKV 800
Cdd:COG5110  755 DKTTLMPKNTAGLFTTVFMLLdssiFPLvsSHalmyFLLCQIRPQKYVTLSEKGEPIKV 813
HEAT_2 pfam13646
HEAT repeats; This family includes multiple HEAT repeats.
 599-690 1.27e-12

HEAT repeats; This family includes multiple HEAT repeats.


Pssm-ID: 433376 [Multi-domain]  Cd Length: 88  Bit Score: 64.28  E-value: 1.27e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353  599 IRRLLHVAVSDVNDDVRRAAVESLGfiLFRTPEQCPSVVSLLSESyNPHVRYGAAMALGIccagTGNKEAINLLEPM-TN 677
Cdd:pfam13646   1 LPALLQALLRDPDPEVRAAAIRALG--RIGDPEAVPALLELLKDE-DPAVRRAAAEALGK----IGDPEALPALLELlRD 73

                          90
                  ....*....|...
gi 119591353  678 DPVNYVRQGALIA 690
Cdd:pfam13646  74 DDDDVVRAAAAEA 86
HEAT COG1413
HEAT repeat [General function prediction only];
545-690 2.15e-12

HEAT repeat [General function prediction only];


Pssm-ID: 441023 [Multi-domain]  Cd Length: 137  Bit Score: 65.42  E-value: 2.15e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 545 LRGLAVGiALVMYGRMEEADALIESLcRDKDPILRRSGMYTVAMAycgsGNNKAIRRLLHvAVSDVNDDVRRAAVESLGF 624
Cdd:COG1413    1 VRRAAAR-ALGRLGDPAAVPALIAAL-ADEDPDVRAAAARALGRL----GDPRAVPALLE-ALKDPDPEVRAAAAEALGR 73

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 119591353 625 IlfRTPEQCPSVVSLLSESyNPHVRYGAAMALGiccaGTGNKEAINLLEPMTNDPVNYVRQGALIA 690
Cdd:COG1413   74 I--GDPEAVPALIAALKDE-DPEVRRAAAEALG----RLGDPAAVPALLEALKDPDWEVRRAAARA 132
HEAT COG1413
HEAT repeat [General function prediction only];
510-657 1.19e-10

HEAT repeat [General function prediction only];


Pssm-ID: 441023 [Multi-domain]  Cd Length: 137  Bit Score: 60.41  E-value: 1.19e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 510 EAAGLALGLVmlgsKNAQAIEDMVGYAQETQHEkiLRGLAVgIALVMYGRMEEADALIESLcRDKDPILRRSgmytVAMA 589
Cdd:COG1413    3 RAAARALGRL----GDPAAVPALIAALADEDPD--VRAAAA-RALGRLGDPRAVPALLEAL-KDPDPEVRAA----AAEA 70

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 119591353 590 YCGSGNNKAIRRLLHvAVSDVNDDVRRAAVESLGFIlfRTPEQCPSVVSLLSESyNPHVRYGAAMALG 657
Cdd:COG1413   71 LGRIGDPEAVPALIA-ALKDEDPEVRRAAAEALGRL--GDPAAVPALLEALKDP-DWEVRRAAARALG 134
HEAT_2 pfam13646
HEAT repeats; This family includes multiple HEAT repeats.
 563-657 2.56e-10

HEAT repeats; This family includes multiple HEAT repeats.


Pssm-ID: 433376 [Multi-domain]  Cd Length: 88  Bit Score: 57.73  E-value: 2.56e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353  563 ADALIESLCRDKDPILRRsgmyTVAMAyCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFIlfRTPEQCPSVVSLLSE 642
Cdd:pfam13646   1 LPALLQALLRDPDPEVRA----AAIRA-LGRIGDPEAVPALLELLKDEDPAVRRAAAEALGKI--GDPEALPALLELLRD 73

                          90
                  ....*....|....*
gi 119591353  643 SYNPHVRYGAAMALG 657
Cdd:pfam13646  74 DDDDVVRAAAAEALA 88
PC_rep pfam01851
Proteasome/cyclosome repeat;
651-685 6.21e-07

Proteasome/cyclosome repeat;


Pssm-ID: 460361 [Multi-domain]  Cd Length: 35  Bit Score: 46.64  E-value: 6.21e-07
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 119591353  651 GAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQ 685
Cdd:pfam01851   1 GAALALGLIHAGSGNEEALDLLLPYLSDTSAESRA 35
HEAT COG1413
HEAT repeat [General function prediction only];
614-694 7.74e-06

HEAT repeat [General function prediction only];


Pssm-ID: 441023 [Multi-domain]  Cd Length: 137  Bit Score: 46.55  E-value: 7.74e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119591353 614 VRRAAVESLGFIlfRTPEQCPSVVSLLSESyNPHVRYGAAMALGiccaGTGNKEAINLLEPMTNDPVNYVRQGALIASAL 693
Cdd:COG1413    1 VRRAAARALGRL--GDPAAVPALIAALADE-DPDVRAAAARALG----RLGDPRAVPALLEALKDPDPEVRAAAAEALGR 73


                 .
gi 119591353 694 I 694
Cdd:COG1413   74 I 74
PC_rep pfam01851
Proteasome/cyclosome repeat;
511-544 1.64e-04

Proteasome/cyclosome repeat;


Pssm-ID: 460361 [Multi-domain]  Cd Length: 35  Bit Score: 39.71  E-value: 1.64e-04
                          10        20        30
                  ....*....|....*....|....*....|....
gi 119591353  511 AAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKI 544
Cdd:pfam01851   1 GAALALGLIHAGSGNEEALDLLLPYLSDTSAESR 34
HEAT_2 pfam13646
HEAT repeats; This family includes multiple HEAT repeats.
 545-623 1.86e-04

HEAT repeats; This family includes multiple HEAT repeats.


Pssm-ID: 433376 [Multi-domain]  Cd Length: 88  Bit Score: 41.17  E-value: 1.86e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 119591353  545 LRGLAVgIALVMYGRMEEADALIEsLCRDKDPILRRsgmyTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLG 623
Cdd:pfam13646  16 VRAAAI-RALGRIGDPEAVPALLE-LLKDEDPAVRR----AAAEALGKIGDPEALPALLELLRDDDDDVVRAAAAEALA 88
PC_rep pfam01851
Proteasome/cyclosome repeat;
476-510 7.22e-04

Proteasome/cyclosome repeat;


Pssm-ID: 460361 [Multi-domain]  Cd Length: 35  Bit Score: 37.78  E-value: 7.22e-04
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 119591353  476 GGSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGE 510
Cdd:pfam01851   1 GAALALGLIHAGSGNEEALDLLLPYLSDTSAESRA 35
PC_rep pfam01851
Proteasome/cyclosome repeat;
441-474 7.29e-04

Proteasome/cyclosome repeat;


Pssm-ID: 460361 [Multi-domain]  Cd Length: 35  Bit Score: 37.78  E-value: 7.29e-04
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 119591353  441 GGLYALGLIHANHG-GDIIDYLLNQLKNASNDIVR 474
Cdd:pfam01851   1 GAALALGLIHAGSGnEEALDLLLPYLSDTSAESRA 35
HEAT_EZ pfam13513
HEAT-like repeat; The HEAT repeat family is related to armadillo/beta-catenin-like repeats ...
 646-690 2.10e-03

HEAT-like repeat; The HEAT repeat family is related to armadillo/beta-catenin-like repeats (see pfam00514). These EZ repeats are found in subunits of cyanobacterial phycocyanin lyase and other proteins and probably carry out a scaffolding role.


Pssm-ID: 463906 [Multi-domain]  Cd Length: 55  Bit Score: 36.96  E-value: 2.10e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 119591353  646 PHVRYGAAMALGICCAGTGN------KEAINLLEPMTNDPVNYVRQGALIA 690
Cdd:pfam13513   1 WRVREAAALALGSLAEGGPDllapavPELLPALLPLLNDDSDLVREAAAWA 51
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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