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Conserved domains on  [gi|41393551|ref|NP_060631|]
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glutamine-dependent NAD(+) synthetase [Homo sapiens]

Protein Classification

glutamine-dependent NAD(+) synthetase( domain architecture ID 1003107)

glutamine-dependent NAD(+) synthetase catalyzes the ATP-dependent amidation of deamido-NAD to form NAD; uses L-glutamine as a nitrogen source

CATH:  3.40.50.620|3.60.110.10
EC:  6.3.5.1
Gene Ontology:  GO:0004359|GO:0003952|GO:0005524|GO:0009435

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PLN02339 super family cl31864
NAD+ synthase (glutamine-hydrolysing)
 3-699 0e+00

NAD+ synthase (glutamine-hydrolysing)


The actual alignment was detected with superfamily member PLN02339:

Pssm-ID: 177973 [Multi-domain]  Cd Length: 700  Bit Score: 1095.89  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551    3 RKVTVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVLAALVESPVTQDI 82
Cdd:PLN02339   2 RLLKVATCNLNQWAMDFDGNLKRIKESIAEAKAAGAVYRVGPELEITGYGCEDHFLELDTVTHSWECLAEILVGDLTDGI 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   83 ICDVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSRHTEEYFLPRMIQDLTKQETVPFGDAV 162
Cdd:PLN02339  82 LCDIGMPVIHGGVRYNCRVFCLNRKILLIRPKMWLANDGNYRELRWFTAWKHKKKVEDFQLPEEIAEATSQKSVPFGDGY 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  163 LVTWDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGGIYLLANQKGCDGDRLYY 242
Cdd:PLN02339 162 LQFLDTAVAAETCEELFTPQAPHIDLALNGVEIISNGSGSHHQLRKLNTRLDLIRSATHKCGGVYLYANQRGCDGGRLYY 241

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  243 DGCAMIAMNGSVFAQGSQFSLDDVEVLTATLDLEDVRSYRAEISSRNLAASRASPYPRVKVDFALSCHEDLLAPISEPIE 322
Cdd:PLN02339 242 DGCACIVVNGEVVAQGSQFSLQDVEVVTACVDLDAVVSFRGSISSFREQASSKKRVPSVAVPFKLCPPFSLSLVPSSPLK 321

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  323 WKYHSPEEEISLGPACWLWDFLRRSQQAGFLLPLSGGVDSAATACLIYSMCCQVCEAVRSGNEEVLADVRTIVNQIS-YT 401
Cdd:PLN02339 322 IRYHSPEEEIALGPACWLWDYLRRSGASGFLLPLSGGADSSSVAAIVGSMCQLVVKAIREGDEQVKADARRIGNYADgEV 401

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  402 PQDPRDLCGRILTTCYMASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPLFAAHGGSSRENLAL 481
Cdd:PLN02339 402 PTDSKEFAKRIFYTVYMGSENSSEETRSRAKQLADEIGSSHLDVKIDGVVSAVLSLFQTLTGKRPRYKVDGGSNAENLAL 481

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  482 QNVQARIRMVLAYLFAQLSLWSRGVHGGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCIQRFQLP 561
Cdd:PLN02339 482 QNIQARIRMVLAFMLASLLPWVRGKSGFLLVLGSANVDEGLRGYLTKYDCSSADINPIGGISKQDLRSFLRWAATNLGYP 561

                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  562 ALQSILLAPATAELEPLADGQvSQTDEEDMGMTYAELSVYGKLRKVAKMGPYSMFCKLLGMWRHICTPRQVADKVKRFFS 641
Cdd:PLN02339 562 SLAEVEAAPPTAELEPIRDDY-SQTDEEDMGMTYEELGVYGRLRKIFRCGPVSMFKNLCHEWNGRLSPSEVAAKVKDFFK 640

                        650       660       670       680       690
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 41393551  642 KYSMNRHKMTTLTPAYHAENYSPEDNRFDLRPFLYNTSWPWQFRCIENQVLQLERAEP 699
Cdd:PLN02339 641 YYSINRHKMTTLTPSYHAESYSPDDNRFDLRQFLYNTRWPYQFRKIDELVEELDGETV 698
 
Name Accession Description Interval E-value
PLN02339 PLN02339
NAD+ synthase (glutamine-hydrolysing)
 3-699 0e+00

NAD+ synthase (glutamine-hydrolysing)


Pssm-ID: 177973 [Multi-domain]  Cd Length: 700  Bit Score: 1095.89  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551    3 RKVTVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVLAALVESPVTQDI 82
Cdd:PLN02339   2 RLLKVATCNLNQWAMDFDGNLKRIKESIAEAKAAGAVYRVGPELEITGYGCEDHFLELDTVTHSWECLAEILVGDLTDGI 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   83 ICDVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSRHTEEYFLPRMIQDLTKQETVPFGDAV 162
Cdd:PLN02339  82 LCDIGMPVIHGGVRYNCRVFCLNRKILLIRPKMWLANDGNYRELRWFTAWKHKKKVEDFQLPEEIAEATSQKSVPFGDGY 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  163 LVTWDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGGIYLLANQKGCDGDRLYY 242
Cdd:PLN02339 162 LQFLDTAVAAETCEELFTPQAPHIDLALNGVEIISNGSGSHHQLRKLNTRLDLIRSATHKCGGVYLYANQRGCDGGRLYY 241

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  243 DGCAMIAMNGSVFAQGSQFSLDDVEVLTATLDLEDVRSYRAEISSRNLAASRASPYPRVKVDFALSCHEDLLAPISEPIE 322
Cdd:PLN02339 242 DGCACIVVNGEVVAQGSQFSLQDVEVVTACVDLDAVVSFRGSISSFREQASSKKRVPSVAVPFKLCPPFSLSLVPSSPLK 321

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  323 WKYHSPEEEISLGPACWLWDFLRRSQQAGFLLPLSGGVDSAATACLIYSMCCQVCEAVRSGNEEVLADVRTIVNQIS-YT 401
Cdd:PLN02339 322 IRYHSPEEEIALGPACWLWDYLRRSGASGFLLPLSGGADSSSVAAIVGSMCQLVVKAIREGDEQVKADARRIGNYADgEV 401

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  402 PQDPRDLCGRILTTCYMASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPLFAAHGGSSRENLAL 481
Cdd:PLN02339 402 PTDSKEFAKRIFYTVYMGSENSSEETRSRAKQLADEIGSSHLDVKIDGVVSAVLSLFQTLTGKRPRYKVDGGSNAENLAL 481

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  482 QNVQARIRMVLAYLFAQLSLWSRGVHGGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCIQRFQLP 561
Cdd:PLN02339 482 QNIQARIRMVLAFMLASLLPWVRGKSGFLLVLGSANVDEGLRGYLTKYDCSSADINPIGGISKQDLRSFLRWAATNLGYP 561

                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  562 ALQSILLAPATAELEPLADGQvSQTDEEDMGMTYAELSVYGKLRKVAKMGPYSMFCKLLGMWRHICTPRQVADKVKRFFS 641
Cdd:PLN02339 562 SLAEVEAAPPTAELEPIRDDY-SQTDEEDMGMTYEELGVYGRLRKIFRCGPVSMFKNLCHEWNGRLSPSEVAAKVKDFFK 640

                        650       660       670       680       690
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 41393551  642 KYSMNRHKMTTLTPAYHAENYSPEDNRFDLRPFLYNTSWPWQFRCIENQVLQLERAEP 699
Cdd:PLN02339 641 YYSINRHKMTTLTPSYHAESYSPDDNRFDLRQFLYNTRWPYQFRKIDELVEELDGETV 698
GAT_Gln-NAD-synth cd07570
Glutamine aminotransferase (GAT, glutaminase) domain of glutamine-dependent NAD synthetases ...
 6-298 2.18e-94

Glutamine aminotransferase (GAT, glutaminase) domain of glutamine-dependent NAD synthetases (class 7 and 8 nitrilases); Glutamine-dependent NAD synthetases are bifunctional enzymes, which have an N-terminal GAT domain and a C-terminal NAD+ synthetase domain. The GAT domain is a glutaminase (EC 3.5.1.2) which hydrolyses L-glutamine to L-glutamate and ammonia. The ammonia is used by the NAD+ synthetase domain in the ATP-dependent amidation of nicotinic acid adenine dinucleotide. Glutamine aminotransferases are categorized depending on their active site residues into different unrelated classes. This class of GAT domain belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to classes 7 and 8. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer. Mycobacterium tuberculosis glutamine-dependent NAD+ synthetase forms a homooctamer.


Pssm-ID: 143594 [Multi-domain]  Cd Length: 261  Bit Score: 293.22  E-value: 2.18e-94
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   6 TVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVLAALVESPVTQDIICD 85
Cdd:cd07570   1 RIALAQLNPTVGDLEGNAEKILEAIREAKAQGADLVVFPELSLTGYPPEDLLLRPDFLEAAEEALEELAAATADLDIAVV 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  86 VGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSrhteeyflprmiqdltkqetvpfgdAVLVT 165
Cdd:cd07570  81 VGLPLRHDGKLYNAAAVLQNGKILGVVPKQLLPNYGVFDEKRYFTPGDKP-------------------------DVLFF 135

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 166 WDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGGIYLLANQkGCDGDRLYYDGC 245
Cdd:cd07570 136 KGLRIGVEICEDLWVPDPPSAELALAGADLILNLSASPFHLGKQDYRRELVSSRSARTGLPYVYVNQ-VGGQDDLVFDGG 214

                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....
gi 41393551 246 AMIAMN-GSVFAQGSQFslddvEVLTATLDLEDVRSYRAEISSrnLAASRASPY 298
Cdd:cd07570 215 SFIADNdGELLAEAPRF-----EEDLADVDLDRLRSERRRNSS--FLDEEAEIY 261
CN_hydrolase pfam00795
Carbon-nitrogen hydrolase; This family contains hydrolases that break carbon-nitrogen bonds. ...
 6-283 1.21e-50

Carbon-nitrogen hydrolase; This family contains hydrolases that break carbon-nitrogen bonds. The family includes: Nitrilase EC:3.5.5.1, Aliphatic amidase EC:3.5.1.4, Biotidinase EC:3.5.1.12, Beta-ureidopropionase EC:3.5.1.6. Nitrilase-related proteins generally have a conserved E-K-C catalytic triad, and are multimeric alpha-beta-beta-alpha sandwich proteins.


Pssm-ID: 425873 [Multi-domain]  Cd Length: 257  Bit Score: 177.16  E-value: 1.21e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551     6 TVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTlLHSFQVLAALVESPVTQDIICD 85
Cdd:pfam00795   1 RVALVQLPQGFWDLEANLQKALELIEEAARYGADLIVLPELFITGYPCWAHFLEAAE-VGDGETLAGLAALARKNGIAIV 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551    86 VGmpVMHRNVrYNCRviFLNRKILLIRPKMALaneGNYRELRWFTPWSRSRHTEEyflprmiqdltkqETVPFGDAVLV- 164
Cdd:pfam00795  80 IG--LIERWL-TGGR--LYNTAVLLDPDGKLV---GKYRKLHLFPEPRPPGFRER-------------VLFEPGDGGTVf 138

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   165 -TWDTCIGSEICEELWTPHSPHIdMGLDGVEIITNAS-GSHQVLRKANTRVDLVTMVTSKNGGIYLL-ANQKGCDGD-RL 240
Cdd:pfam00795 139 dTPLGKIGAAICYEIRFPELLRA-LALKGAEILINPSaRAPFPGSLGPPQWLLLARARALENGCFVIaANQVGGEEDaPW 217

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|...
gi 41393551   241 YYDGCAMIAMNGSVFAQGSQFSLddvEVLTATLDLEDVRSYRA 283
Cdd:pfam00795 218 PYGHSMIIDPDGRILAGAGEWEE---GVLIADIDLALVRAWRY 257
NadE COG0171
NH3-dependent NAD+ synthetase [Coenzyme transport and metabolism]; NH3-dependent NAD+ ...
 68-658 5.94e-43

NH3-dependent NAD+ synthetase [Coenzyme transport and metabolism]; NH3-dependent NAD+ synthetase is part of the Pathway/BioSystem: NAD biosynthesis


Pssm-ID: 439941 [Multi-domain]  Cd Length: 542  Bit Score: 163.48  E-value: 5.94e-43
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  68 QVLAALVESPVTQDIICDVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWsrsRHTEEYFLPRMI 147
Cdd:COG0171  20 AAAAALAALAAAAAALLLLLLLLLLLLLLLLALLLLLAAARLLVAAAALLLLALAAGGGAALAGG---GGGAGGGLLNGA 96

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 148 QDLTKQETVPFGDAVLVTWDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGGIY 227
Cdd:COG0171  97 ALVLGGGDLLFFADDFLLLLLVVEEEEEEFVGGPPPPPAALGGAGVLLILSSSSSAVGAAAAAAALAAALLSSLSSAAYY 176

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 228 LLANQKGCDGDRLYYDGCAMIAMNGSVFAQGSQFSLDDVEVLTATLDLEDVRSYRAEISSRNLAASRASPYPRvkvdfal 307
Cdd:COG0171 177 AAAGGGESTTDLARGGGGGLLLVVLLLVGGGDDDFFDGGSAAVDDDDLLLLLRRRREEELLLARARDADGGRR------- 249

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 308 sCHEDLLAPISEPIEWKYHSPEEEISLGpacwLWDFLRRSQQAGFLLPLSGGVDSAATACLiysmccqVCEAVrsGNEEV 387
Cdd:COG0171 250 -VAAEAAPPPPEEEEMDLEEVYDALVLG----LRDYVRKNGFKGVVLGLSGGIDSALVAAL-------AVDAL--GPENV 315

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 388 LAdvrtivnqisytpqdprdlcgrilttCYMASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPl 467
Cdd:COG0171 316 LG--------------------------VTMPSRYTSDESLEDAEELAENLGIEYEEIDITPAVEAFLEALPHAFGGEL- 368

                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 468 faahggssrENLALQNVQARIRMVLAYLFAQLslwsrgvHGGlLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDL 547
Cdd:COG0171 369 ---------DDVAEENLQARIRMVILMALANK-------FGG-LVLGTGNKSELAVGYFTKYGDGAGDLAPIADLYKTQV 431

                       490       500       510       520       530       540       550       560
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 548 RAFVQFcIQRFQLPALQSILLAPATAELEPladgqvSQTDEEDMGmTYAELSvygklrkvakmgpysmFCkLLGMWRHIC 627
Cdd:COG0171 432 YALARW-LNRNGEVIPEDIIDKPPSAELRP------GQTDEDELG-PYEVLD----------------AI-LYAYVEEGL 486

                       570       580       590
                ....*....|....*....|....*....|....*..
gi 41393551 628 TPRQVA------DKVKRFFSKYSMNRHKMTTLTPAYH 658
Cdd:COG0171 487 SPEEIAaagydrEWVERVLRLVRRNEYKRRQPPPGPK 523
nadE TIGR00552
NAD+ synthetase; NAD+ synthetase is a nearly ubiquitous enzyme for the final step in the ...
 340-665 1.39e-35

NAD+ synthetase; NAD+ synthetase is a nearly ubiquitous enzyme for the final step in the biosynthesis of the essensial cofactor NAD. The member of this family from Bacillus subtilis is a strictly NH(3)-dependent NAD(+) synthetase of 272 amino acids. Proteins consisting only of the domain modeled here may be named as NH3-dependent NAD+ synthetase. Amidotransferase activity may reside in a separate protein, or not be present. Some other members of the family, such as from Mycobacterium tuberculosis, are considerably longer, contain an apparent amidotransferase domain, and show glutamine-dependent as well as NH(3)-dependent activity. [Biosynthesis of cofactors, prosthetic groups, and carriers, Pyridine nucleotides]


Pssm-ID: 273132 [Multi-domain]  Cd Length: 250  Bit Score: 134.82  E-value: 1.39e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   340 LWDFLR----RSQQAGFLLPLSGGVDSAATACLiysmccqvceAVRSGNEEVLAdvrtivnqisytpqdprdlcGRIltt 415
Cdd:TIGR00552   9 IEDFLRgyvqKSGAKGVVLGLSGGIDSAVVAAL----------CVEALGEQNHA--------------------LLL--- 55

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   416 cyMASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPLfaahggssrenLALQNVQARIRMVLAYL 495
Cdd:TIGR00552  56 --PHSVQTPEQDVQDALALAEPLGINYKNIDIAPIAASFQAQTETGDELSDF-----------LAKGNLKARLRMAALYA 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   496 FAQLSlwsrgvhgGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVqfciQRFQLPalQSILLAPATAEL 575
Cdd:TIGR00552 123 IANKH--------NLLVLGTGNKSELMLGYFTKYGDGGCDIAPIGDLFKTQVYELA----KRLNVP--ERIIEKPPTADL 188

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   576 EPladgqvSQTDEEDMGMTYAELSVYGKLRKVAKmgpysmfckllgmwrhictpRQVADKVKRFFSKYSMNRHKMTtlTP 655
Cdd:TIGR00552 189 FD------GQTDETELGITYDELDDYLKGIEELS--------------------QTVQEVVKRIESLVQKSEHKRR--LP 240

                         330
                  ....*....|
gi 41393551   656 AYHAENYSPE 665
Cdd:TIGR00552 241 ATIFDLFWKQ 250
 
Name Accession Description Interval E-value
PLN02339 PLN02339
NAD+ synthase (glutamine-hydrolysing)
 3-699 0e+00

NAD+ synthase (glutamine-hydrolysing)


Pssm-ID: 177973 [Multi-domain]  Cd Length: 700  Bit Score: 1095.89  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551    3 RKVTVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVLAALVESPVTQDI 82
Cdd:PLN02339   2 RLLKVATCNLNQWAMDFDGNLKRIKESIAEAKAAGAVYRVGPELEITGYGCEDHFLELDTVTHSWECLAEILVGDLTDGI 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   83 ICDVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSRHTEEYFLPRMIQDLTKQETVPFGDAV 162
Cdd:PLN02339  82 LCDIGMPVIHGGVRYNCRVFCLNRKILLIRPKMWLANDGNYRELRWFTAWKHKKKVEDFQLPEEIAEATSQKSVPFGDGY 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  163 LVTWDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGGIYLLANQKGCDGDRLYY 242
Cdd:PLN02339 162 LQFLDTAVAAETCEELFTPQAPHIDLALNGVEIISNGSGSHHQLRKLNTRLDLIRSATHKCGGVYLYANQRGCDGGRLYY 241

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  243 DGCAMIAMNGSVFAQGSQFSLDDVEVLTATLDLEDVRSYRAEISSRNLAASRASPYPRVKVDFALSCHEDLLAPISEPIE 322
Cdd:PLN02339 242 DGCACIVVNGEVVAQGSQFSLQDVEVVTACVDLDAVVSFRGSISSFREQASSKKRVPSVAVPFKLCPPFSLSLVPSSPLK 321

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  323 WKYHSPEEEISLGPACWLWDFLRRSQQAGFLLPLSGGVDSAATACLIYSMCCQVCEAVRSGNEEVLADVRTIVNQIS-YT 401
Cdd:PLN02339 322 IRYHSPEEEIALGPACWLWDYLRRSGASGFLLPLSGGADSSSVAAIVGSMCQLVVKAIREGDEQVKADARRIGNYADgEV 401

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  402 PQDPRDLCGRILTTCYMASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPLFAAHGGSSRENLAL 481
Cdd:PLN02339 402 PTDSKEFAKRIFYTVYMGSENSSEETRSRAKQLADEIGSSHLDVKIDGVVSAVLSLFQTLTGKRPRYKVDGGSNAENLAL 481

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  482 QNVQARIRMVLAYLFAQLSLWSRGVHGGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCIQRFQLP 561
Cdd:PLN02339 482 QNIQARIRMVLAFMLASLLPWVRGKSGFLLVLGSANVDEGLRGYLTKYDCSSADINPIGGISKQDLRSFLRWAATNLGYP 561

                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  562 ALQSILLAPATAELEPLADGQvSQTDEEDMGMTYAELSVYGKLRKVAKMGPYSMFCKLLGMWRHICTPRQVADKVKRFFS 641
Cdd:PLN02339 562 SLAEVEAAPPTAELEPIRDDY-SQTDEEDMGMTYEELGVYGRLRKIFRCGPVSMFKNLCHEWNGRLSPSEVAAKVKDFFK 640

                        650       660       670       680       690
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 41393551  642 KYSMNRHKMTTLTPAYHAENYSPEDNRFDLRPFLYNTSWPWQFRCIENQVLQLERAEP 699
Cdd:PLN02339 641 YYSINRHKMTTLTPSYHAESYSPDDNRFDLRQFLYNTRWPYQFRKIDELVEELDGETV 698
GAT_Gln-NAD-synth cd07570
Glutamine aminotransferase (GAT, glutaminase) domain of glutamine-dependent NAD synthetases ...
 6-298 2.18e-94

Glutamine aminotransferase (GAT, glutaminase) domain of glutamine-dependent NAD synthetases (class 7 and 8 nitrilases); Glutamine-dependent NAD synthetases are bifunctional enzymes, which have an N-terminal GAT domain and a C-terminal NAD+ synthetase domain. The GAT domain is a glutaminase (EC 3.5.1.2) which hydrolyses L-glutamine to L-glutamate and ammonia. The ammonia is used by the NAD+ synthetase domain in the ATP-dependent amidation of nicotinic acid adenine dinucleotide. Glutamine aminotransferases are categorized depending on their active site residues into different unrelated classes. This class of GAT domain belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to classes 7 and 8. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer. Mycobacterium tuberculosis glutamine-dependent NAD+ synthetase forms a homooctamer.


Pssm-ID: 143594 [Multi-domain]  Cd Length: 261  Bit Score: 293.22  E-value: 2.18e-94
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   6 TVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVLAALVESPVTQDIICD 85
Cdd:cd07570   1 RIALAQLNPTVGDLEGNAEKILEAIREAKAQGADLVVFPELSLTGYPPEDLLLRPDFLEAAEEALEELAAATADLDIAVV 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  86 VGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSrhteeyflprmiqdltkqetvpfgdAVLVT 165
Cdd:cd07570  81 VGLPLRHDGKLYNAAAVLQNGKILGVVPKQLLPNYGVFDEKRYFTPGDKP-------------------------DVLFF 135

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 166 WDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGGIYLLANQkGCDGDRLYYDGC 245
Cdd:cd07570 136 KGLRIGVEICEDLWVPDPPSAELALAGADLILNLSASPFHLGKQDYRRELVSSRSARTGLPYVYVNQ-VGGQDDLVFDGG 214

                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....
gi 41393551 246 AMIAMN-GSVFAQGSQFslddvEVLTATLDLEDVRSYRAEISSrnLAASRASPY 298
Cdd:cd07570 215 SFIADNdGELLAEAPRF-----EEDLADVDLDRLRSERRRNSS--FLDEEAEIY 261
NAD_synthase cd00553
NAD(+) synthase; NAD(+) synthase (EC 6.3.5.1), a homodimer, catalyzes the final step in the de ...
 327-650 1.95e-91

NAD(+) synthase; NAD(+) synthase (EC 6.3.5.1), a homodimer, catalyzes the final step in the de novo nicotinamide adenine dinucleotide (NAD(+)) biosynthesis, an amide transfer from either ammonia or glutamine to nicotinic acid adenine dinucleotide (NaAD). The conversion of NaAD to NAD(+) occurs via a NAD-adenylate intermediate and requires ATP and Mg(2+). The intermediate is subsequently cleaved into NAD(+) and AMP. In many prokaryotes, such as Escherichia coli, NAD synthase consists of a single domain and is strictly ammonia dependent. In contrast, eukaryotes and other prokaryotes have an additional N-terminal amidohydrolase domain that prefer glutamine. Interestingly, NAD(+) synthases in these prokaryotes, can also utilize ammonia as an amide source.


Pssm-ID: 467484 [Multi-domain]  Cd Length: 248  Bit Score: 284.83  E-value: 1.95e-91
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 327 SPEEEISLGPACWLWDFLRRSQQAGFLLPLSGGVDSAATACLIYSMCCqvceavrsgneevladvrtivnqisytpqdpr 406
Cdd:cd00553   1 IDPEEIIEALVCFLRDYLRKSGAKGFVLGLSGGIDSAVVAALAVRALG-------------------------------- 48

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 407 dlCGRILTTCYMaSKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVtgksplfaahGGSSRENLALQNVQA 486
Cdd:cd00553  49 --AENVLALIMP-SRYSSKETRDDAKALAENLGIEYRTIDIDPIVDAFLKALEHA----------GGSEAEDLALGNIQA 115

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 487 RIRMVLAYLFAQLSlwsrgvhgGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCIqrfqlpALQSI 566
Cdd:cd00553 116 RLRMVLLYALANLL--------GGLVLGTGNKSELLLGYFTKYGDGAADINPIGDLYKTQVRELARYLG------VPEEI 181

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 567 LLAPATAELEPladgqvSQTDEEDMGMTYAELSVYGKLRKVAKMGPYSmfckllgmwrhICTPRQVADKVKRFFSKYSMN 646
Cdd:cd00553 182 IEKPPSAELWP------GQTDEDELGMPYEELDLILYGLVDGKLGPEE-----------ILSPGEDEEKVKRIFRLYRRN 244


                ....
gi 41393551 647 RHKM 650
Cdd:cd00553 245 EHKR 248
CN_hydrolase pfam00795
Carbon-nitrogen hydrolase; This family contains hydrolases that break carbon-nitrogen bonds. ...
 6-283 1.21e-50

Carbon-nitrogen hydrolase; This family contains hydrolases that break carbon-nitrogen bonds. The family includes: Nitrilase EC:3.5.5.1, Aliphatic amidase EC:3.5.1.4, Biotidinase EC:3.5.1.12, Beta-ureidopropionase EC:3.5.1.6. Nitrilase-related proteins generally have a conserved E-K-C catalytic triad, and are multimeric alpha-beta-beta-alpha sandwich proteins.


Pssm-ID: 425873 [Multi-domain]  Cd Length: 257  Bit Score: 177.16  E-value: 1.21e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551     6 TVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTlLHSFQVLAALVESPVTQDIICD 85
Cdd:pfam00795   1 RVALVQLPQGFWDLEANLQKALELIEEAARYGADLIVLPELFITGYPCWAHFLEAAE-VGDGETLAGLAALARKNGIAIV 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551    86 VGmpVMHRNVrYNCRviFLNRKILLIRPKMALaneGNYRELRWFTPWSRSRHTEEyflprmiqdltkqETVPFGDAVLV- 164
Cdd:pfam00795  80 IG--LIERWL-TGGR--LYNTAVLLDPDGKLV---GKYRKLHLFPEPRPPGFRER-------------VLFEPGDGGTVf 138

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   165 -TWDTCIGSEICEELWTPHSPHIdMGLDGVEIITNAS-GSHQVLRKANTRVDLVTMVTSKNGGIYLL-ANQKGCDGD-RL 240
Cdd:pfam00795 139 dTPLGKIGAAICYEIRFPELLRA-LALKGAEILINPSaRAPFPGSLGPPQWLLLARARALENGCFVIaANQVGGEEDaPW 217

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|...
gi 41393551   241 YYDGCAMIAMNGSVFAQGSQFSLddvEVLTATLDLEDVRSYRA 283
Cdd:pfam00795 218 PYGHSMIIDPDGRILAGAGEWEE---GVLIADIDLALVRAWRY 257
NadE COG0171
NH3-dependent NAD+ synthetase [Coenzyme transport and metabolism]; NH3-dependent NAD+ ...
 68-658 5.94e-43

NH3-dependent NAD+ synthetase [Coenzyme transport and metabolism]; NH3-dependent NAD+ synthetase is part of the Pathway/BioSystem: NAD biosynthesis


Pssm-ID: 439941 [Multi-domain]  Cd Length: 542  Bit Score: 163.48  E-value: 5.94e-43
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  68 QVLAALVESPVTQDIICDVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWsrsRHTEEYFLPRMI 147
Cdd:COG0171  20 AAAAALAALAAAAAALLLLLLLLLLLLLLLLALLLLLAAARLLVAAAALLLLALAAGGGAALAGG---GGGAGGGLLNGA 96

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 148 QDLTKQETVPFGDAVLVTWDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGGIY 227
Cdd:COG0171  97 ALVLGGGDLLFFADDFLLLLLVVEEEEEEFVGGPPPPPAALGGAGVLLILSSSSSAVGAAAAAAALAAALLSSLSSAAYY 176

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 228 LLANQKGCDGDRLYYDGCAMIAMNGSVFAQGSQFSLDDVEVLTATLDLEDVRSYRAEISSRNLAASRASPYPRvkvdfal 307
Cdd:COG0171 177 AAAGGGESTTDLARGGGGGLLLVVLLLVGGGDDDFFDGGSAAVDDDDLLLLLRRRREEELLLARARDADGGRR------- 249

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 308 sCHEDLLAPISEPIEWKYHSPEEEISLGpacwLWDFLRRSQQAGFLLPLSGGVDSAATACLiysmccqVCEAVrsGNEEV 387
Cdd:COG0171 250 -VAAEAAPPPPEEEEMDLEEVYDALVLG----LRDYVRKNGFKGVVLGLSGGIDSALVAAL-------AVDAL--GPENV 315

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 388 LAdvrtivnqisytpqdprdlcgrilttCYMASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPl 467
Cdd:COG0171 316 LG--------------------------VTMPSRYTSDESLEDAEELAENLGIEYEEIDITPAVEAFLEALPHAFGGEL- 368

                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 468 faahggssrENLALQNVQARIRMVLAYLFAQLslwsrgvHGGlLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDL 547
Cdd:COG0171 369 ---------DDVAEENLQARIRMVILMALANK-------FGG-LVLGTGNKSELAVGYFTKYGDGAGDLAPIADLYKTQV 431

                       490       500       510       520       530       540       550       560
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 548 RAFVQFcIQRFQLPALQSILLAPATAELEPladgqvSQTDEEDMGmTYAELSvygklrkvakmgpysmFCkLLGMWRHIC 627
Cdd:COG0171 432 YALARW-LNRNGEVIPEDIIDKPPSAELRP------GQTDEDELG-PYEVLD----------------AI-LYAYVEEGL 486

                       570       580       590
                ....*....|....*....|....*....|....*..
gi 41393551 628 TPRQVA------DKVKRFFSKYSMNRHKMTTLTPAYH 658
Cdd:COG0171 487 SPEEIAaagydrEWVERVLRLVRRNEYKRRQPPPGPK 523
NAD_synthase pfam02540
NAD synthase; NAD synthase (EC:6.3.5.1) is involved in the de novo synthesis of NAD and is ...
 339-649 1.82e-36

NAD synthase; NAD synthase (EC:6.3.5.1) is involved in the de novo synthesis of NAD and is induced by stress factors such as heat shock and glucose limitation.


Pssm-ID: 396888 [Multi-domain]  Cd Length: 241  Bit Score: 137.13  E-value: 1.82e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   339 WLWDFLRRSQQAGFLLPLSGGVDSAATACLiysmcCQvcEAVrsGNEEVLAdvrtivnqisytpqdprdlcgrilttCYM 418
Cdd:pfam02540   8 FLRDYVQKAGFKGVVLGLSGGIDSSLVAYL-----AV--KAL--GKENVLA--------------------------LIM 52

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   419 ASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAvmgifslvtgksplFAAHGGSSRENLALQNVQARIRMVLAYLFAQ 498
Cdd:pfam02540  53 PSSQSSEEDVQDALALAENLGIEYKTIDIKPIVRA--------------FSQLFQDASEDFAKGNLKARIRMAILYYIAN 118

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   499 LSlwsrgvhgGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVqfciQRFQLPalQSILLAPATAELEPl 578
Cdd:pfam02540 119 KF--------NYLVLGTGNKSELAVGYFTKYGDGACDIAPIGDLYKTQVYELA----RYLNVP--ERIIKKPPSADLWP- 183

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 41393551   579 adgqvSQTDEEDMGMTYAELSVYGKLRKvAKMGPYSMFCKLLgmwrhictPRQVadkVKRFFSKYSMNRHK 649
Cdd:pfam02540 184 -----GQTDEEELGIPYDELDDILKLVE-KKLSPEEIIGKGL--------PAEV---VRRIENLIQKSEHK 237
nadE PRK02628
NAD synthetase; Reviewed
 4-592 6.32e-36

NAD synthetase; Reviewed


Pssm-ID: 235057 [Multi-domain]  Cd Length: 679  Bit Score: 144.24  E-value: 6.32e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551    4 KVTVATCALNqwALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDhYYESDTLLH-SFQVLAALVESPVTQDI 82
Cdd:PRK02628  14 RVAAATPKVR--VADPAFNAARILALARRAADDGVALAVFPELSLSGYSCDD-LFLQDTLLDaVEDALATLVEASADLDP 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   83 ICDVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRsrhteeyfLPRMIQDLTKQEtVPFGDAV 162
Cdd:PRK02628  91 LLVVGAPLRVRHRLYNCAVVIHRGRILGVVPKSYLPNYREFYEKRWFAPGDG--------ARGETIRLCGQE-VPFGTDL 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  163 LVTWDT----CIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGGIYLLANqKGC--- 235
Cdd:PRK02628 162 LFEAEDlpgfVFGVEICEDLWVPIPPSSYAALAGATVLANLSASNITVGKADYRRLLVASQSARCLAAYVYAA-AGVges 240

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  236 --DgdrLYYDGCAMIAMNGSVFAQGSQFSlDDVEVLTATLDLEDVRSYRAEISSRNLAASRASPYPR-VKVDFALSCHED 312
Cdd:PRK02628 241 ttD---LAWDGQTLIYENGELLAESERFP-REEQLIVADVDLERLRQERLRNGSFDDNARHRDESAPfRTIPFALDPPAG 316

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  313 LLaPISEPIEWKYHSPEEEISLGPACW-LWDF-----LRRSQQAG---FLLPLSGGVDSaaTACLIysmccqVCeavrsg 383
Cdd:PRK02628 317 DL-GLRRPVERFPFVPSDPARLDQRCYeAYNIqvsglAQRLRATGlkkVVIGISGGLDS--THALL------VA------ 381

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  384 neevladVRTIvnqisytpqdprDLCGR----ILtTCYMASKNSSQETCTRARELAQQIGSHHISLNIDPAVK---AVMG 456
Cdd:PRK02628 382 -------AKAM------------DRLGLprknIL-AYTMPGFATTDRTKNNAVALMKALGVTAREIDIRPAALqmlKDIG 441

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  457 ifslvtgksplfaaHGGSSREN---LALQNVQARIRMvlAYLF--AQLslwsrgvHGGlLVLGSANVDESLLGYLTkYDC 531
Cdd:PRK02628 442 --------------HPFARGEPvydVTFENVQAGERT--QILFrlANQ-------HGG-IVIGTGDLSELALGWCT-YGV 496

                        570       580       590       600       610       620       630
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 41393551  532 ----SSADINpiGGISKTDLRAFVQFCIQRFQLPA-----LQSILLAPATAELEP-LADGQVSQTDEEDMG 592
Cdd:PRK02628 497 gdhmSHYNVN--ASVPKTLIQHLIRWVIASGQFDEavsevLLDILDTEISPELVPaDKEGEIVQSTEDIIG 565
nadE TIGR00552
NAD+ synthetase; NAD+ synthetase is a nearly ubiquitous enzyme for the final step in the ...
 340-665 1.39e-35

NAD+ synthetase; NAD+ synthetase is a nearly ubiquitous enzyme for the final step in the biosynthesis of the essensial cofactor NAD. The member of this family from Bacillus subtilis is a strictly NH(3)-dependent NAD(+) synthetase of 272 amino acids. Proteins consisting only of the domain modeled here may be named as NH3-dependent NAD+ synthetase. Amidotransferase activity may reside in a separate protein, or not be present. Some other members of the family, such as from Mycobacterium tuberculosis, are considerably longer, contain an apparent amidotransferase domain, and show glutamine-dependent as well as NH(3)-dependent activity. [Biosynthesis of cofactors, prosthetic groups, and carriers, Pyridine nucleotides]


Pssm-ID: 273132 [Multi-domain]  Cd Length: 250  Bit Score: 134.82  E-value: 1.39e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   340 LWDFLR----RSQQAGFLLPLSGGVDSAATACLiysmccqvceAVRSGNEEVLAdvrtivnqisytpqdprdlcGRIltt 415
Cdd:TIGR00552   9 IEDFLRgyvqKSGAKGVVLGLSGGIDSAVVAAL----------CVEALGEQNHA--------------------LLL--- 55

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   416 cyMASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPLfaahggssrenLALQNVQARIRMVLAYL 495
Cdd:TIGR00552  56 --PHSVQTPEQDVQDALALAEPLGINYKNIDIAPIAASFQAQTETGDELSDF-----------LAKGNLKARLRMAALYA 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   496 FAQLSlwsrgvhgGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVqfciQRFQLPalQSILLAPATAEL 575
Cdd:TIGR00552 123 IANKH--------NLLVLGTGNKSELMLGYFTKYGDGGCDIAPIGDLFKTQVYELA----KRLNVP--ERIIEKPPTADL 188

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   576 EPladgqvSQTDEEDMGMTYAELSVYGKLRKVAKmgpysmfckllgmwrhictpRQVADKVKRFFSKYSMNRHKMTtlTP 655
Cdd:TIGR00552 189 FD------GQTDETELGITYDELDDYLKGIEELS--------------------QTVQEVVKRIESLVQKSEHKRR--LP 240

                         330
                  ....*....|
gi 41393551   656 AYHAENYSPE 665
Cdd:TIGR00552 241 ATIFDLFWKQ 250
PRK13981 PRK13981
NAD synthetase; Provisional
 5-588 1.37e-28

NAD synthetase; Provisional


Pssm-ID: 237577 [Multi-domain]  Cd Length: 540  Bit Score: 120.65  E-value: 1.37e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551    5 VTVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVLAALVESpVTQDIIC 84
Cdd:PRK13981   1 LRIALAQLNPTVGDIAGNAAKILAAAAEAADAGADLLLFPELFLSGYPPEDLLLRPAFLAACEAALERLAAA-TAGGPAV 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   85 DVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSrhteeyflprmiqdltkqETVPFGDAVlv 164
Cdd:PRK13981  80 LVGHPWREGGKLYNAAALLDGGEVLATYRKQDLPNYGVFDEKRYFAPGPEP------------------GVVELKGVR-- 139

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  165 twdtcIGSEICEELWTPhSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGG--IYLlaNQKGcdG-DRLY 241
Cdd:PRK13981 140 -----IGVPICEDIWNP-EPAETLAEAGAELLLVPNASPYHRGKPDLREAVLRARVRETGLplVYL--NQVG--GqDELV 209

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  242 YDGCAmIAMN--GSVFAQGSQFsldDVEVLTATLDLEDVRsyraeissrnlaasraspyprvkvdfaLSCHEDLLAPISE 319
Cdd:PRK13981 210 FDGAS-FVLNadGELAARLPAF---EEQIAVVDFDRGEDG---------------------------WRPLPGPIAPPPE 258

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  320 PIEWKYHSpeeeISLGpacwLWDFLRRSQQAGFLLPLSGGVDSAATACLiysmccqVCEAVrsGNEEVLAdvrtivnqis 399
Cdd:PRK13981 259 GEAEDYRA----LVLG----LRDYVRKNGFPGVVLGLSGGIDSALVAAI-------AVDAL--GAERVRA---------- 311

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  400 ytpqdprdlcgrilttCYMASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFslvtgkSPLFAAHGgssrENL 479
Cdd:PRK13981 312 ----------------VMMPSRYTSEESLDDAAALAKNLGVRYDIIPIEPAFEAFEAAL------APLFAGTE----PDI 365

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  480 ALQNVQARIR----MVLAYLFaqlslwsrgvhgGLLVLGSANVDESLLGYLTKYdcssADIN----PIGGISKTDLrafv 551
Cdd:PRK13981 366 TEENLQSRIRgtllMALSNKF------------GSLVLTTGNKSEMAVGYATLY----GDMAggfaPIKDVYKTLV---- 425

                        570       580       590       600
                 ....*....|....*....|....*....|....*....|....*...
gi 41393551  552 qfciqrFQLPAL-----------QSILLAPATAELEPladgqvSQTDE 588
Cdd:PRK13981 426 ------YRLCRWrntvspgevipERIITKPPSAELRP------NQTDQ 461
PRK13980 PRK13980
NAD synthetase; Provisional
 342-598 9.54e-28

NAD synthetase; Provisional


Pssm-ID: 184435 [Multi-domain]  Cd Length: 265  Bit Score: 113.00  E-value: 9.54e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  342 DFLRRSQQAGFLLPLSGGVDSAATACLiysmccqvceAVRS-GNEEVLAdvrtivnqisytpqdprdlcgrilttCYMAS 420
Cdd:PRK13980  23 EEVEKAGAKGVVLGLSGGIDSAVVAYL----------AVKAlGKENVLA--------------------------LLMPS 66

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  421 KNSSQETCTRARELAQQIGSHHISLNIDPAVKAvmgifslvtgkspLFAAHGGSSRenLALQNVQARIRMVLAYLFAQls 500
Cdd:PRK13980  67 SVSPPEDLEDAELVAEDLGIEYKVIEITPIVDA-------------FFSAIPDADR--LRVGNIMARTRMVLLYDYAN-- 129

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  501 lwsrgvHGGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRafvqfciqrfQLPAL----QSILLAPATAELE 576
Cdd:PRK13980 130 ------RENRLVLGTGNKSELLLGYFTKYGDGAVDLNPIGDLYKTQVR----------ELARHlgvpEDIIEKPPSADLW 193

                        250       260
                 ....*....|....*....|..
gi 41393551  577 PladgqvSQTDEEDMGMTYAEL 598
Cdd:PRK13980 194 E------GQTDEGELGFSYETI 209
Nit2 COG0388
Omega-amidase YafV/Nit2, hydrolyzes alpha-ketoglutaramate [Energy production and conversion];
4-287 8.59e-27

Omega-amidase YafV/Nit2, hydrolyzes alpha-ketoglutaramate [Energy production and conversion];


Pssm-ID: 440157 [Multi-domain]  Cd Length: 264  Bit Score: 109.95  E-value: 8.59e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551   4 KVTVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYEsdtllhsfqVLAALVESPVTQ--- 80
Cdd:COG0388   1 TMRIALAQLNPTVGDIEANLAKIEELIREAAAQGADLVVFPELFLTGYPPEDDDLL---------ELAEPLDGPALAala 71

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  81 ------DIICDVGMPV-MHRNVRYNCRVIFLNR-KILLIRPKMALANEGNYRELRWFTPwsrsrhteeyflprmiqdltk 152
Cdd:COG0388  72 elarelGIAVVVGLPErDEGGRLYNTALVIDPDgEILGRYRKIHLPNYGVFDEKRYFTP--------------------- 130

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 153 qetvpfGDAvLVTWDT---CIGSEICEELWTPHSPHIdMGLDGVEIITNASGSHQVLRKAntRVDLVTMVTSKNGGIYLL 229
Cdd:COG0388 131 ------GDE-LVVFDTdggRIGVLICYDLWFPELARA-LALAGADLLLVPSASPFGRGKD--HWELLLRARAIENGCYVV 200

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 230 -ANQKGCDGDRLYYdGCAMIA-MNGSVFAQGSqfslDDVEVLTATLDLEDVRSYRAEISS 287
Cdd:COG0388 201 aANQVGGEDGLVFD-GGSMIVdPDGEVLAEAG----DEEGLLVADIDLDRLREARRRFPV 255
nitrilase_8 cd07586
Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The ...
 18-286 1.88e-12

Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.


Pssm-ID: 143610  Cd Length: 269  Bit Score: 68.08  E-value: 1.88e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  18 DFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSfQVLAALVESPVTQDIICdvGMPVMHRNVR- 96
Cdd:cd07586  13 DVEENLEKHLEIIETARERGADLVVFPELSLTGYNLGDLVYEVAMHADD-PRLQALAEASGGICVVF--GFVEEGRDGRf 89

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  97 YNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSRHTEEYFLpRMiqdltkqetvpfgdavlvtwdtciGSEICE 176
Cdd:cd07586  90 YNSAAYLEDGRVVHVHRKVYLPTYGLFEEGRYFAPGSHLRAFDTRFG-RA------------------------GVLICE 144

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551 177 ELWTPHSPHIDMgLDGVEII---TNASGSHQVLRKANTRV--DLVTMVTSKNGGIYLLANQKGCDGDRLYYDGCAMIAMN 251
Cdd:cd07586 145 DAWHPSLPYLLA-LDGADVIfipANSPARGVGGDFDNEENweTLLKFYAMMNGVYVVFANRVGVEDGVYFWGGSRVVDPD 223

                       250       260       270
                ....*....|....*....|....*....|....*
gi 41393551 252 GSVFAQGSQFSLDdveVLTATLDLEDVRSYRAEIS 286
Cdd:cd07586 224 GEVVAEAPLFEED---LLVAELDRSAIRRARFFSP 255
nadE PRK00768
ammonia-dependent NAD(+) synthetase;
440-603 6.61e-11

ammonia-dependent NAD(+) synthetase;


Pssm-ID: 234831 [Multi-domain]  Cd Length: 268  Bit Score: 63.62  E-value: 6.61e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  440 SHHISLNIDPAVKAVMGifSLvtgksplfaAHGGSSRENLALQNVQARIRMVLAYLFAqlslwsrGVHGGLlVLGSANVD 519
Cdd:PRK00768 102 DRVLTVNIKPAVDASVA--AL---------EAAGIELSDFVKGNIKARERMIAQYAIA-------GATGGL-VVGTDHAA 162

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 41393551  520 ESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFciqrfqLPALQSILLAPATAELEplaDGQVSQTDEEDMGMTYAELS 599
Cdd:PRK00768 163 EAVTGFFTKFGDGGADILPLFGLNKRQGRALLAA------LGAPEHLYEKVPTADLE---DDRPGLPDEVALGVTYDQID 233


                 ....*.
gi 41393551  600 VY--GK 603
Cdd:PRK00768 234 DYleGK 239
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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