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Conserved domains on  [gi|296452861|sp|Q2M389|]
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RecName: Full=WASH complex subunit 4; AltName: Full=Strumpellin and WASH-interacting protein; Short=SWIP; AltName: Full=WASH complex subunit SWIP

Protein Classification

WASH-4_N and WASH-7_mid domain-containing protein( domain architecture ID 10631708)

protein containing domains WASH-4_N, WASH-7_mid, and WASH-7_C

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
WASH-4_N pfam14745
WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins ...
32-600 0e+00

WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins that form subunit 4 of the WASH complex, also known as subunit 7 or KIAA1033/SWIP. In species such as Drosophila this protein is the only component of the 'complex'. This complex is present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. This domain comprises the WAHD1 and the TBR (tubulin-binding region) domains.


:

Pssm-ID: 434178  Cd Length: 566  Bit Score: 825.80  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861    32 GKFLEEYTSQLRRIEDALDDSIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETK 111
Cdd:pfam14745    1 GSFMEEYAQKLWRLEEALDDSISDVWDFVLDPISLNYLPYEQISLLELIKTDNKILNKVLTVLASLCNEIRLLKEEAESK 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   112 FYNGLLFYGEGATDASMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIETTGVHFQTMYE 191
Cdd:pfam14745   81 FYQALFLYGEGEEESNAGEGDAQVEMSKLLPFLQDLSCFVNRCYEVLHNLVQQLAALYTNKKKNPRPIDVNEVHLQTVFE 160
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   192 HLGELLTVLLTLDEIIDNHITLKDHWTMYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDS 271
Cdd:pfam14745  161 HLGDLLTVLVTLDEIIDNQSALSEHWKLYKRMLKSVRHNPSKFGVAEEKLKQLEKLLKDLEGQLLSGNIFQICIEQLFDS 240
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   272 lnggVSVSKNSTFAEEFAHSIRSIFANVEAKLGEPSEIDQRDKYVGICGLFVLHFQIFRTIDKKFYKSLLDICKKVPAIT 351
Cdd:pfam14745  241 ----VDVKKNKNLSEEFAAYIKNLFTDVEAKIGDTNELDERKKWIGILGLYVLHIKLFGTCDKKLFKSVWDVQKKIPAVH 316
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   352 LTANIIWFPDNFLIQKIPAAAKLLDRKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESILSKEQRMDKFAE 431
Cdd:pfam14745  317 LVGNIIWFPSDFLSQHLPKLIKILDKKQTEAIKQLRESYLQKRDQSLPRDVQSLYLQVSLWMVRMESILTKTVLANGFFE 396
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   432 DLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLVELLKAIEHMFYRRSMVVADSVSHITQHLQHQ 511
Cdd:pfam14745  397 DLKSRCKLLIQGLLLASQISHLIKTVINLHVALGKPMTKSDVLLICKLIEMLKSIEHTFHRRQILIVRSLSSLLQYLSYL 476
                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   512 ALHSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALS-VGTQMKTFKDEELFPLQVVMKKLDLI 590
Cdd:pfam14745  477 ILHLLETAKKRLQSDKKYSERKLDVLSALKLAEKLLNGPPTKERRLIIRLALSeVIQQNKSFKEDELVKLRSILKRLDTI 556
                          570
                   ....*....|
gi 296452861   591 SELRERVQTQ 600
Cdd:pfam14745  557 SDLQENIQEQ 566
WASH-7_mid pfam14744
WASH complex subunit 7; This family is the central, conserved region of proteins that form ...
605-949 0e+00

WASH complex subunit 7; This family is the central, conserved region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes.


:

Pssm-ID: 434177  Cd Length: 346  Bit Score: 635.41  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   605 FLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHARHLESY-EILLDCYDKEIMEILNEHLLDKLCKEIEKD 683
Cdd:pfam14744    1 FLYWHRELLPLYLSDVYENPLQASRIQYLLSALSDCVPLLKSAKHLENPgKGLLDSYEEEIEKALKEEILEPLCRDIETD 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   684 LRLSVHTHLKLDDRNPFKVGMKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYG 763
Cdd:pfam14744   81 LRLHVHSHLLLDDRNPFKSGVKDLSRFLRLKPLRLFDKFIDIKAEVEHYLESTFYNLTTVALHDWKTYEEMRNLAKLKYG 160
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   764 LVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTY 843
Cdd:pfam14744  161 LNLLDPHLPGQTLEQGLDVLEIMRNIHVFVSRYNYNLNNQIFVERSSDSKHLNTIGIRHIANSIRTHGTGIMNTTVNFTY 240
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   844 QFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREIKDQNDHKYPFDRAEKFNRGIRKLGVTPEGQSYLDQFRQLISQIGNAM 923
Cdd:pfam14744  241 QFLRKKFYIFSQFLFDDHIKSRLLKDLRFFRENKKELDGKYPYERAEKFIKGIRKLGLGDDGLSYLDQFRILITQIGNAL 320
                          330       340
                   ....*....|....*....|....*.
gi 296452861   924 GYVRMIRSGGLHCSSNAIRFVPDLED 949
Cdd:pfam14744  321 GYVRMLRSGGLHACSNAIRFVPDLEE 346
WASH-7_C pfam14746
WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins ...
966-1135 2.09e-93

WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes. The C-terminus is predicted to include a transmembrane region.


:

Pssm-ID: 464293  Cd Length: 175  Bit Score: 296.08  E-value: 2.09e-93
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   966 TLKAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSISCKEKLNKKNKIGAAF 1045
Cdd:pfam14746    1 TVKAARNLDKVISSLKKNFSEGTDYFKLLVDVFAPELRSPKNMHLRNFYLIVPALTLNYVEHMISAKEKLLKKNKVGASF 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861  1046 TDDGFAMGVAYILKLLDQYREFDSLHWFQSVREKYLKEIRAV-----AKQQNVQSASQDEKLLQTMNLTQKRLDVYLQEF 1120
Cdd:pfam14746   81 TDDGFAMGLAYILKLLDQYEEFDSLHWFESVKKKYNEELEDVqeilaAQSKQASAQKEDEKLQQTMHLTLKRLEAYLREF 160
                          170
                   ....*....|....*
gi 296452861  1121 ELLYFSLSSARIFFR 1135
Cdd:pfam14746  161 DLLFYSLSSARIFFR 175
 
Name Accession Description Interval E-value
WASH-4_N pfam14745
WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins ...
32-600 0e+00

WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins that form subunit 4 of the WASH complex, also known as subunit 7 or KIAA1033/SWIP. In species such as Drosophila this protein is the only component of the 'complex'. This complex is present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. This domain comprises the WAHD1 and the TBR (tubulin-binding region) domains.


Pssm-ID: 434178  Cd Length: 566  Bit Score: 825.80  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861    32 GKFLEEYTSQLRRIEDALDDSIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETK 111
Cdd:pfam14745    1 GSFMEEYAQKLWRLEEALDDSISDVWDFVLDPISLNYLPYEQISLLELIKTDNKILNKVLTVLASLCNEIRLLKEEAESK 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   112 FYNGLLFYGEGATDASMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIETTGVHFQTMYE 191
Cdd:pfam14745   81 FYQALFLYGEGEEESNAGEGDAQVEMSKLLPFLQDLSCFVNRCYEVLHNLVQQLAALYTNKKKNPRPIDVNEVHLQTVFE 160
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   192 HLGELLTVLLTLDEIIDNHITLKDHWTMYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDS 271
Cdd:pfam14745  161 HLGDLLTVLVTLDEIIDNQSALSEHWKLYKRMLKSVRHNPSKFGVAEEKLKQLEKLLKDLEGQLLSGNIFQICIEQLFDS 240
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   272 lnggVSVSKNSTFAEEFAHSIRSIFANVEAKLGEPSEIDQRDKYVGICGLFVLHFQIFRTIDKKFYKSLLDICKKVPAIT 351
Cdd:pfam14745  241 ----VDVKKNKNLSEEFAAYIKNLFTDVEAKIGDTNELDERKKWIGILGLYVLHIKLFGTCDKKLFKSVWDVQKKIPAVH 316
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   352 LTANIIWFPDNFLIQKIPAAAKLLDRKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESILSKEQRMDKFAE 431
Cdd:pfam14745  317 LVGNIIWFPSDFLSQHLPKLIKILDKKQTEAIKQLRESYLQKRDQSLPRDVQSLYLQVSLWMVRMESILTKTVLANGFFE 396
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   432 DLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLVELLKAIEHMFYRRSMVVADSVSHITQHLQHQ 511
Cdd:pfam14745  397 DLKSRCKLLIQGLLLASQISHLIKTVINLHVALGKPMTKSDVLLICKLIEMLKSIEHTFHRRQILIVRSLSSLLQYLSYL 476
                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   512 ALHSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALS-VGTQMKTFKDEELFPLQVVMKKLDLI 590
Cdd:pfam14745  477 ILHLLETAKKRLQSDKKYSERKLDVLSALKLAEKLLNGPPTKERRLIIRLALSeVIQQNKSFKEDELVKLRSILKRLDTI 556
                          570
                   ....*....|
gi 296452861   591 SELRERVQTQ 600
Cdd:pfam14745  557 SDLQENIQEQ 566
WASH-7_mid pfam14744
WASH complex subunit 7; This family is the central, conserved region of proteins that form ...
605-949 0e+00

WASH complex subunit 7; This family is the central, conserved region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes.


Pssm-ID: 434177  Cd Length: 346  Bit Score: 635.41  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   605 FLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHARHLESY-EILLDCYDKEIMEILNEHLLDKLCKEIEKD 683
Cdd:pfam14744    1 FLYWHRELLPLYLSDVYENPLQASRIQYLLSALSDCVPLLKSAKHLENPgKGLLDSYEEEIEKALKEEILEPLCRDIETD 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   684 LRLSVHTHLKLDDRNPFKVGMKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYG 763
Cdd:pfam14744   81 LRLHVHSHLLLDDRNPFKSGVKDLSRFLRLKPLRLFDKFIDIKAEVEHYLESTFYNLTTVALHDWKTYEEMRNLAKLKYG 160
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   764 LVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTY 843
Cdd:pfam14744  161 LNLLDPHLPGQTLEQGLDVLEIMRNIHVFVSRYNYNLNNQIFVERSSDSKHLNTIGIRHIANSIRTHGTGIMNTTVNFTY 240
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   844 QFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREIKDQNDHKYPFDRAEKFNRGIRKLGVTPEGQSYLDQFRQLISQIGNAM 923
Cdd:pfam14744  241 QFLRKKFYIFSQFLFDDHIKSRLLKDLRFFRENKKELDGKYPYERAEKFIKGIRKLGLGDDGLSYLDQFRILITQIGNAL 320
                          330       340
                   ....*....|....*....|....*.
gi 296452861   924 GYVRMIRSGGLHCSSNAIRFVPDLED 949
Cdd:pfam14744  321 GYVRMLRSGGLHACSNAIRFVPDLEE 346
WASH-7_C pfam14746
WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins ...
966-1135 2.09e-93

WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes. The C-terminus is predicted to include a transmembrane region.


Pssm-ID: 464293  Cd Length: 175  Bit Score: 296.08  E-value: 2.09e-93
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   966 TLKAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSISCKEKLNKKNKIGAAF 1045
Cdd:pfam14746    1 TVKAARNLDKVISSLKKNFSEGTDYFKLLVDVFAPELRSPKNMHLRNFYLIVPALTLNYVEHMISAKEKLLKKNKVGASF 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861  1046 TDDGFAMGVAYILKLLDQYREFDSLHWFQSVREKYLKEIRAV-----AKQQNVQSASQDEKLLQTMNLTQKRLDVYLQEF 1120
Cdd:pfam14746   81 TDDGFAMGLAYILKLLDQYEEFDSLHWFESVKKKYNEELEDVqeilaAQSKQASAQKEDEKLQQTMHLTLKRLEAYLREF 160
                          170
                   ....*....|....*
gi 296452861  1121 ELLYFSLSSARIFFR 1135
Cdd:pfam14746  161 DLLFYSLSSARIFFR 175
 
Name Accession Description Interval E-value
WASH-4_N pfam14745
WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins ...
32-600 0e+00

WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins that form subunit 4 of the WASH complex, also known as subunit 7 or KIAA1033/SWIP. In species such as Drosophila this protein is the only component of the 'complex'. This complex is present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. This domain comprises the WAHD1 and the TBR (tubulin-binding region) domains.


Pssm-ID: 434178  Cd Length: 566  Bit Score: 825.80  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861    32 GKFLEEYTSQLRRIEDALDDSIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETK 111
Cdd:pfam14745    1 GSFMEEYAQKLWRLEEALDDSISDVWDFVLDPISLNYLPYEQISLLELIKTDNKILNKVLTVLASLCNEIRLLKEEAESK 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   112 FYNGLLFYGEGATDASMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIETTGVHFQTMYE 191
Cdd:pfam14745   81 FYQALFLYGEGEEESNAGEGDAQVEMSKLLPFLQDLSCFVNRCYEVLHNLVQQLAALYTNKKKNPRPIDVNEVHLQTVFE 160
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   192 HLGELLTVLLTLDEIIDNHITLKDHWTMYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDS 271
Cdd:pfam14745  161 HLGDLLTVLVTLDEIIDNQSALSEHWKLYKRMLKSVRHNPSKFGVAEEKLKQLEKLLKDLEGQLLSGNIFQICIEQLFDS 240
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   272 lnggVSVSKNSTFAEEFAHSIRSIFANVEAKLGEPSEIDQRDKYVGICGLFVLHFQIFRTIDKKFYKSLLDICKKVPAIT 351
Cdd:pfam14745  241 ----VDVKKNKNLSEEFAAYIKNLFTDVEAKIGDTNELDERKKWIGILGLYVLHIKLFGTCDKKLFKSVWDVQKKIPAVH 316
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   352 LTANIIWFPDNFLIQKIPAAAKLLDRKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESILSKEQRMDKFAE 431
Cdd:pfam14745  317 LVGNIIWFPSDFLSQHLPKLIKILDKKQTEAIKQLRESYLQKRDQSLPRDVQSLYLQVSLWMVRMESILTKTVLANGFFE 396
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   432 DLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLVELLKAIEHMFYRRSMVVADSVSHITQHLQHQ 511
Cdd:pfam14745  397 DLKSRCKLLIQGLLLASQISHLIKTVINLHVALGKPMTKSDVLLICKLIEMLKSIEHTFHRRQILIVRSLSSLLQYLSYL 476
                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   512 ALHSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALS-VGTQMKTFKDEELFPLQVVMKKLDLI 590
Cdd:pfam14745  477 ILHLLETAKKRLQSDKKYSERKLDVLSALKLAEKLLNGPPTKERRLIIRLALSeVIQQNKSFKEDELVKLRSILKRLDTI 556
                          570
                   ....*....|
gi 296452861   591 SELRERVQTQ 600
Cdd:pfam14745  557 SDLQENIQEQ 566
WASH-7_mid pfam14744
WASH complex subunit 7; This family is the central, conserved region of proteins that form ...
605-949 0e+00

WASH complex subunit 7; This family is the central, conserved region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes.


Pssm-ID: 434177  Cd Length: 346  Bit Score: 635.41  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   605 FLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHARHLESY-EILLDCYDKEIMEILNEHLLDKLCKEIEKD 683
Cdd:pfam14744    1 FLYWHRELLPLYLSDVYENPLQASRIQYLLSALSDCVPLLKSAKHLENPgKGLLDSYEEEIEKALKEEILEPLCRDIETD 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   684 LRLSVHTHLKLDDRNPFKVGMKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYG 763
Cdd:pfam14744   81 LRLHVHSHLLLDDRNPFKSGVKDLSRFLRLKPLRLFDKFIDIKAEVEHYLESTFYNLTTVALHDWKTYEEMRNLAKLKYG 160
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   764 LVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTY 843
Cdd:pfam14744  161 LNLLDPHLPGQTLEQGLDVLEIMRNIHVFVSRYNYNLNNQIFVERSSDSKHLNTIGIRHIANSIRTHGTGIMNTTVNFTY 240
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   844 QFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREIKDQNDHKYPFDRAEKFNRGIRKLGVTPEGQSYLDQFRQLISQIGNAM 923
Cdd:pfam14744  241 QFLRKKFYIFSQFLFDDHIKSRLLKDLRFFRENKKELDGKYPYERAEKFIKGIRKLGLGDDGLSYLDQFRILITQIGNAL 320
                          330       340
                   ....*....|....*....|....*.
gi 296452861   924 GYVRMIRSGGLHCSSNAIRFVPDLED 949
Cdd:pfam14744  321 GYVRMLRSGGLHACSNAIRFVPDLEE 346
WASH-7_C pfam14746
WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins ...
966-1135 2.09e-93

WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes. The C-terminus is predicted to include a transmembrane region.


Pssm-ID: 464293  Cd Length: 175  Bit Score: 296.08  E-value: 2.09e-93
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861   966 TLKAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSISCKEKLNKKNKIGAAF 1045
Cdd:pfam14746    1 TVKAARNLDKVISSLKKNFSEGTDYFKLLVDVFAPELRSPKNMHLRNFYLIVPALTLNYVEHMISAKEKLLKKNKVGASF 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296452861  1046 TDDGFAMGVAYILKLLDQYREFDSLHWFQSVREKYLKEIRAV-----AKQQNVQSASQDEKLLQTMNLTQKRLDVYLQEF 1120
Cdd:pfam14746   81 TDDGFAMGLAYILKLLDQYEEFDSLHWFESVKKKYNEELEDVqeilaAQSKQASAQKEDEKLQQTMHLTLKRLEAYLREF 160
                          170
                   ....*....|....*
gi 296452861  1121 ELLYFSLSSARIFFR 1135
Cdd:pfam14746  161 DLLFYSLSSARIFFR 175
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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