|
Name |
Accession |
Description |
Interval |
E-value |
| cas_Csn1 |
TIGR01865 |
CRISPR subtype II/NMENI RNA-guided endonuclease Cas9/Csn1; CRISPR loci appear to be mobile ... |
4-1050 |
0e+00 |
|
CRISPR subtype II/NMENI RNA-guided endonuclease Cas9/Csn1; CRISPR loci appear to be mobile elements with a wide host range. This model represents a protein found only in CRISPR-containing species, near other CRISPR-associated proteins (cas), as part of the NMENI subtype of CRISPR/Cas locus. The species range so far for this protein is animal pathogens and commensals only.
Pssm-ID: 273840 Cd Length: 805 Bit Score: 899.48 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 4 KYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNtdrhsiKKNLIGALLFDSGETAE-ATRLKRTARRRYTRRKNRICYL 82
Cdd:TIGR01865 1 EYILGLDIGIASVGWAIVEDDYKVPAAKRLIDGG------VRNFTGAELPKTGETAAlDRRLARGARRRIRRRKHRLLRL 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 83 QEIFSNEMAKVDDSFFHRLEESFLVEEDKKHerhpifgnivdevayhekypTIYHLRKKLVDSTDKADLrlIYLALAHMI 162
Cdd:TIGR01865 75 QELFSREGSLTDFDFFSRLENSFLVEEDKRN--------------------TIYHLRKAALENKLKPDE--LYLALLHII 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 163 KFRGHFLIEgdlnpdnsdvdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfgnli 242
Cdd:TIGR01865 133 KHRGHFLIE----------------------------------------------------------------------- 141
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 243 alslgltpnfksnfdlaedaklqlskdtyDDDLDnllaqigdqyadlflaaknlsdaillsdilrVNTEITKAPLSASMI 322
Cdd:TIGR01865 142 -----------------------------GNDFD-------------------------------TANKETGALLSAVMI 161
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 323 KRYDEHHQDLTLLKALVRQQLPEKYKEIFFDqskngyagyidggasqeefykfikpilekmdgteellvklnreDLLRKQ 402
Cdd:TIGR01865 162 NRYLEHEADLRTLKELILKKFPKKYKEIFSE-------------------------------------------TFLRNQ 198
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 403 RTFDNGSIPHQIHLGELHAILRRQEDFYPFlkdnrekiEKILTFRIPYYVGPLARGNSRFAwmtrkseetitpwnfeeVV 482
Cdd:TIGR01865 199 RGFYNGSIPRQLLLEELEAIFRKQREYYPF--------IKLLTFRIPYYIGPLAEGKSEFA-----------------FV 253
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 483 DKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVK 562
Cdd:TIGR01865 254 DKPASAENFIEKMTGKCTYLPEEKRAPKHSLLAEKFTVLNELNNVRIIILEQGETKILSKEEKQELLDLLFKKKKLTYKK 333
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 563 QLKEDYFKKIECFDSVEISGVEDR---FNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY 639
Cdd:TIGR01865 334 LRKLLGLSEDAIFKGLRYEGLDNAekaFNISLKTYHKLRKALGDKDLLDNPKNPKDLDEIVKILTLYKDREMIKKRLELY 413
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 640 AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAqvsgqgds 719
Cdd:TIGR01865 414 KDVLNEEQVKKLVRLHFTGWGRLSLKALRGIRPLMEQGKRYDEAILELGGNRNFMQNINDSQLLPKINITKA-------- 485
|
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 720 lhehiANLAGSPAIKKGILQTVKVVDELVKVMGrhKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGS----QI 795
Cdd:TIGR01865 486 -----KDEILNPVVKRALLQARKVVNELVKKYG--PPDKIVIEMAREEQGTNFGKRNSKERYKKNEDKIKEFASalgkEI 558
|
810 820 830 840 850 860 870 880
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 796 LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRL---SDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS 872
Cdd:TIGR01865 559 LKEEPTENSSKNILKLRLYYQQNGKCMYTGKEIDIDDLfdlSYYEIDHILPQSRSFDDSISNKVLVLASENQEKGDQTPY 638
|
890 900 910 920 930 940 950 960
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 873 E-EVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDendklIR 951
Cdd:TIGR01865 639 EaEIVKKDSAFWNKFEAYVLISKRKSDKLTRAERGGLSDDDKAGFIDRNLNDTRYITRVVANYLKDRFNFHLK-----KR 713
|
970 980 990 1000 1010 1020 1030 1040
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 952 EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAkseqeigK 1031
Cdd:TIGR01865 714 KVKVVTLKGQLTSQLRKKWGLYKKREINNYHHAHDAYINAVSTNALVKKFSQLEPEFRYKEYHNFDGRKKKK-------S 786
|
1050
....*....|....*....
gi 1559905467 1032 ATAKYFFYSNIMNFFKTEI 1050
Cdd:TIGR01865 787 ATDKKVKFSNPMEFFKQKV 805
|
|
| Csn1 |
cd09643 |
CRISPR/Cas system-associated protein Cas9; CRISPR (Clustered Regularly Interspaced Short ... |
4-1049 |
0e+00 |
|
CRISPR/Cas system-associated protein Cas9; CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Very large protein containing McrA/HNH-nuclease related domain and a RuvC-like nuclease domain; signature gene for type II
Pssm-ID: 187774 [Multi-domain] Cd Length: 799 Bit Score: 885.22 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 4 KYSIGLDIGTNSVGWAVITDEYKVPSKKFKvlgntdrHSIKKNLIGALLFDSGETAE-ATRLKRTARRRYTRRKNRICYL 82
Cdd:cd09643 1 EYILGLDIGIASVGWAIVEDDYKVPAKKMI-------DCGVKIFTGAELFKTGETAAlDRRLARGARRRIRRRKHRLLRL 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 83 QEIFSNEMAKVDDSFFHRLEESFLveedkkherhpifgnivdevAYHEKYPTIYHLRKKLVDSTDKADLrlIYLALAHMI 162
Cdd:cd09643 74 QELFAREGSLTDFDFFSRLEDSFL--------------------EYHKNYPTIYHLRKAALENKLKPDE--LYLALLHII 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 163 KFRGHFLIEGDLNPDNsdvdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfgnli 242
Cdd:cd09643 132 KHRGHFLIEGDEDTTA---------------------------------------------------------------- 147
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 243 alslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlflaaknlsdaillsdilrvnTEITKAPLSASMI 322
Cdd:cd09643 148 -------------------------------------------------------------------DKETGALLSASMI 160
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 323 KRYDEHHQDLTLLKALVRQQLPEKYKEIFFDqskngyagyidggasqeefykfikpilekmdgteellvklnrEDLLRKQ 402
Cdd:cd09643 161 KRYDEHKADLRKLKELIKKEFFKKYKEIFGD------------------------------------------ETFLRNQ 198
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 403 RTFDNGSIPHQIHLGELHAILRRQEDFYPFlkdnrekiEKILTFRIPYYVGPLARGNSRFAWMTRKSEEtitpwnfeevv 482
Cdd:cd09643 199 RGFYNGSIPRQLLLEELEAIFRKQREYYPF--------EKILTFRIPYYIGPLAEGKSEFAWLTRPALS----------- 259
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 483 dkgasaQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEgMRKPAFLSGEQKKAIVDLLFKTNRKVTVK 562
Cdd:cd09643 260 ------EAFIEKMTGKCTYLPEEKRAPKHSLLAEKFTVLNELNNLRIIEE-QGETKILSKEEKQELLDLLFKKNKLTYKQ 332
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 563 QLKEDYFKKIECFDSVEISG--VEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYA 640
Cdd:cd09643 333 KRKLLGLKEEEIFKGLRYEGlkAEKNFNISLKTYHDLRKALGKEFLKDLELNEKILDEIVKILTLYKDREMIEKILELYK 412
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 641 HLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNfmQLIHDDSLTFKEDIQKAQVsgqgdsl 720
Cdd:cd09643 413 DLLNEEQLKKLLKRHFTGWGRLSLKALRGIRPLMEQGKRYDEAILELGGNHN--QKINSDELKFLPIIKKAQV------- 483
|
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 721 hehiANLAGSPAIKKGILQTVKVVDELVKVMGrhKPENIVIEMARENQtTQKGQKNSRERMKRIEEGIKELGS---QILK 797
Cdd:cd09643 484 ----KDEILNPVVKRALLQARKVVNELVKKYG--PPDKIVIEMARENG-TNKGTKNRKKRQKKNEDNIKEAASaleQKLK 556
|
810 820 830 840 850 860 870 880
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 798 EHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRL---SDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEE 874
Cdd:cd09643 557 ELPLDIKSKNILKLRLYYQQNGKCMYTGKEIDIDDLfdlSYYEIDHILPQSRSFDDSISNKVLVLASENQEKGDQTPYEE 636
|
890 900 910 920 930 940 950 960
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 875 VVKKMKNYWRQLLNAKLITQR---KFDNLtKAERgGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDendklIR 951
Cdd:cd09643 637 IVSKMSAFWNKLEAAKLISQRgdsKKDRL-LLEK-GISDDEKAGFIDRNLNDTRYITRVVANYLKDRFNFHLK-----KR 709
|
970 980 990 1000 1010 1020 1030 1040
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 952 EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLEsefVYGDYKVYDVRKMIAKSEQEIgk 1031
Cdd:cd09643 710 KVKVVTLKGQLTSQLRKKWGLYKKREINNYHHAHDAYINAVVTNALVKKFSQLE---RYKEYKRFDSEKGNKKTLDEN-- 784
|
1050
....*....|....*...
gi 1559905467 1032 ataKYFFYSNIMNFFKTE 1049
Cdd:cd09643 785 ---KKFFFANPMNFFKQE 799
|
|
| Cas9_REC |
pfam16592 |
REC lobe of CRISPR-associated endonuclease Cas9; The REC lobe of Cas9 - the CRISPR-associated ... |
181-710 |
0e+00 |
|
REC lobe of CRISPR-associated endonuclease Cas9; The REC lobe of Cas9 - the CRISPR-associated endonuclease Cas9 - includes the REC1 and REC2 domains. REC1 forms an elongated, alpha-helical structure consisting of 25 alpha helices and two beta-sheets, whereas REC2 inserted within REC1 adopts a six-helix bundle structure. The REC lobe and the NUC lobe of Cas9 fold to present a positively charged groove at their interface which accommodates the negatively charged sgRNA:target DNA heteroduplex. CRISPR (clustered regularly interspaced short palindromic repeat)-Cas system occurs naturally in bacteria as a defence against invasion by phages or other mobile genetic elements. Cas9 is targeted to specific genomic locations by sgRNAs or single guide RNAs, in order to complex with invading DNA in order to cleave it and render it inactive.
Pssm-ID: 435447 Cd Length: 539 Bit Score: 575.94 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 181 VDKLFIQLVQTYNQLFEENPINASGVDAKAILSA-RLSKSRRLENLIAQLPGEK-KNGLFGNLIALSLGLTPNFKSNFDL 258
Cdd:pfam16592 1 VEESFQDLLNILYEQLENLELETQNVEIEKILKKtKISKKAKLDELLALPPNEKnSKKIFAEILKLILGNKADFTKIFEL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 259 ------AEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL 332
Cdd:pfam16592 81 ekfveePKKIKLSFSDSNYDEKIEELENQLGDEKAEIILILKKIYDWVVLSDILTVSTDNGKAYLSEAMVNRYDKHKEDL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 333 TLLKALVRQQLPEKYKEIFFDQSKNGYAGYID----GGASQEEFYKFIKPILEKMDGTEE--LLVKLNREDLLRKQRTFD 406
Cdd:pfam16592 161 AQLKKVIKQNLSEKYNDMFRKEKKKGYSAYINgknnGKTSKEDFYKYIKKLINKVETSEAqyILSKIDNENFLPKQRTKS 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 407 NGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGA 486
Cdd:pfam16592 241 NGSIPYQVHLQELKKIIKNQAEYYPFLKENQEKILKLLTFRIPYYVGPLAEKKSKFAWMKRKEQGKIYPWNFEQKVDIDK 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 487 SAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEgmrkpaFLSGEQKKAIVDLLFKTNRKVTVKQLKE 566
Cdd:pfam16592 321 TAEAFITRMTNYCTYLPDEKVLPKNSLLYSKFTVLNELNKIKINGE------KISVELKQDIFNGLFKKNKKVTKKKLKD 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 567 DYFKKIECFDSVEISGV--EDRFNASLGTYHDLLKIIkdKDFLDNEENEDILEDIVLTLTLFEDREMIEERL-KTYAHLF 643
Cdd:pfam16592 395 WLVKEGYNFKAVEIKGFdkENNFNNSLTTYIDLAKIF--GDFLDNPDNEDIIEDIIYWLTLFEDRKILKRRLqKKYSNLL 472
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 644 DDKVMKQLKRRRYTGWGRLSRKLINGIRDKQS---GKTILDFLKSDgfaNRNFMQLIHDDSLTFKEDIQK 710
Cdd:pfam16592 473 TEKQIKQILKLKYKGWGRLSKELLNGIRGADRqgeIKTIIDLLWND---NRNLMQLINDERLSFKEEIEK 539
|
|
| Cas9 |
COG3513 |
CRISPR-Cas system type-II protein Cas9 [Defense mechanisms]; CRISPR-Cas system type-II protein ... |
3-1018 |
8.18e-128 |
|
CRISPR-Cas system type-II protein Cas9 [Defense mechanisms]; CRISPR-Cas system type-II protein Cas9 is part of the Pathway/BioSystem: CRISPR-Cas system
Pssm-ID: 442735 [Multi-domain] Cd Length: 812 Bit Score: 418.98 E-value: 8.18e-128
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 3 KKYSIGLDIGTNSVGWAVITDEYKVpskkfkvlgntdrHSIKKNLIGALLFDSGET-------AEATRLKRTARRRYTRR 75
Cdd:COG3513 2 DKYILGLDLGINSVGWAVLELDEDG-------------EPGEIIDAGVRIFDDGEDpksgeslAAARREARGARRRRRRR 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 76 KNRICYLQEIFSNEMakvddsffhrleesFLVEEDKKHERHPifgnivdevayhekYPTIYHLRKKLVDstDKADLRLIY 155
Cdd:COG3513 69 KHRLRRLKRLLVEEG--------------LLPADDAERKALL--------------PLNPYELRAKALD--EKLSPEELG 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 156 LALAHMIKFRGHfliegdLNPDNSDVDKLfiqlvqtynqlfeenpinasgvDAKAILSARLSKSRRLENLIAQLPGEkkn 235
Cdd:COG3513 119 RALFHLAQRRGF------KSNRKTDSKDN----------------------ESGKVKDAIKELRERLEAKGARTVGE--- 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 236 glfgnlialslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlFLAaknlsdaillsdilrvnteitka 315
Cdd:COG3513 168 ------------------------------------------------------YLY----------------------- 170
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 316 plsasmiKRYDEHHQdltllkalvrqqlpekykeiffdqskngyagyidggasqeefykfikpilekmdgteellvklnr 395
Cdd:COG3513 171 -------RRLQENGK----------------------------------------------------------------- 178
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 396 edlLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDN--REKIEKILTFRIPYYVGplargnsrfawmtrkseeti 473
Cdd:COG3513 179 ---VRNRKGDYDFYIPREDLEDEFEAIWAAQAEFGPALLTEelRDELLEIIFFQRPLKSG-------------------- 235
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 474 tpwnfeevvdkgasaqsfiERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGmRKPAFLSGEQKKAIVDLLF 553
Cdd:COG3513 236 -------------------KKLVGKCTFEPDEKRAPKASPLFQRFRILQKLNNLRIVDDG-GEERPLTLEERQKIIDLLE 295
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 554 KtNRKVTVKQLKEDYfkKIEcfDSVEISGVEDRFN-----ASLGTYHDLLKIIKDKDFldNEENEDILEDIVLTLTLFED 628
Cdd:COG3513 296 N-KKKLTFKKLRKLL--GLP--DGVIFKGFNYEDDdraklKGDKTYAKLAKIFGKAWL--NEFDPEILDDIVEALTLFKD 368
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 629 REMIEERLKTYAHLfDDKVMKQLKRRR-YTGWGRLSRKLINGIrdkqsgktiLDFLKSDgfanrnfmqlihddsLTFKED 707
Cdd:COG3513 369 DEELKEWLKKLYGL-DEEQAEALANLPlPDGYGNLSLKALRKI---------LPLLEEG---------------LDYDEA 423
|
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 708 IQKAQVSGQGDSLH--------EHIANLAGSPAIKKGILQTVKVVDELVKVMGrhKPENIVIEMARENQTTQKGQKNSRE 779
Cdd:COG3513 424 VKAAGYDHSSLEILdrlppigeEKRKGSIRNPVVHRALNQLRKVVNALIRKYG--KPDEIHIELARDLKKSKKERKEIQK 501
|
810 820 830 840 850 860 870 880
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 780 RMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSD--YDVDHIVPQSFLKDDSIDNKVL 857
Cdd:COG3513 502 RQRENEKAREKAREEIAEEGGGEPSRRDILKYRLWEEQNGRCPYTGKPISISDLLDgsVEIDHILPRSRTLDDSFNNKVL 581
|
890 900 910 920 930 940 950 960
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 858 TRSDKNRGKSDNVPSEEVVK----KMKNYWRQLLNAKLITQRKFDNLTKAERGglsELDKAGFIKRQLVETRQITKHVAQ 933
Cdd:COG3513 582 CLADANREKGNRTPYEALGGdeaeKWEEILARVENLKLIPQKKKKRFLKKELD---RDDDEGFIARQLNDTRYISRLAAE 658
|
970 980 990 1000 1010 1020 1030 1040
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 934 ILDSRMNTKYdendklIREVKVITLKSKLVSDFRKDFQFYKV-------REINNYHHAHDAYLNAVVGTALIKKYPKLES 1006
Cdd:COG3513 659 YLKSLYPFED------KGKRKVRVVPGQLTAMLRRAWGLNKIlsddgekNRDDHRHHAIDALVIACTTQGLLQRLAKASR 732
|
1050
....*....|..
gi 1559905467 1007 EFVYGDYKVYDV 1018
Cdd:COG3513 733 EREDAEKAEEHF 744
|
|
| Cas9_PI |
pfam16595 |
PAM-interacting domain of CRISPR-associated endonuclease Cas9; Cas9_PI is a family found at ... |
1102-1358 |
5.82e-47 |
|
PAM-interacting domain of CRISPR-associated endonuclease Cas9; Cas9_PI is a family found at the C-terminal of bacterial type II CRISPR system Cas9 endonuclease. This domain adopts a novel protein fold that is unique to the Cas9 family. It is positioned in the structure-DNA-complex to recognize the PAM sequence on the non-complementary DNA strand of the crRNA. PAM sequence is protospacer-adjacent motifs on DNA. See family CRISPR-DR2, Rfam:RF01315. Cas9 carries two nuclease domains, HNH and RuvC, which cleave the DNA strands that are complementary and non-complementary to the 20 nucleotide guide sequence in crRNAs, respectively.
Pssm-ID: 435449 Cd Length: 264 Bit Score: 169.81 E-value: 5.82e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1102 TGGFSKESILP--KRNSDKLIARKKD---WDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLksvkeLLGITIMERSSFEK 1176
Cdd:pfam16595 1 KGGLFNQTILPahKKKGKGLIPLKKDergLDVEKYGGYSSLTAAYFSLVEYTGKKGKRKRT-----IEGVPLYLAAKIEE 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1177 NPI--DFLEAKGYKEVKKDLIIKLPKYSLFElENGRKRMLASAGE---LQKGNELALPSKYVNFLYLASHYEKLKGSPED 1251
Cdd:pfam16595 76 NKDllEYLEEKLGLKEPKIILPKIKKNSLIK-IDGFRMLLTGKTEnrlLKNAVQLVLSNDDEKYIKKIEKFVKKNKDDII 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1252 NEQKQLFVEQHKHYLDEIIEQISEFSKrVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA-PAAFKYFDTT 1330
Cdd:pfam16595 155 EEKDGLTEEKNIKLYDELLDKMKNTIY-YKRPSNQGEKLEKLKEKFIKLSLEEKCKVLIEILKLTHANPtSADLKLIGGS 233
|
250 260 270
....*....|....*....|....*....|.
gi 1559905467 1331 IDRKRYTSTKEVLDA---TLIHQSITGLYET 1358
Cdd:pfam16595 234 KHAGRIKISNNISKAsniKLINQSVTGLYEK 264
|
|
| yhbS |
COG3153 |
Predicted N-acetyltransferase YhbS [General function prediction only]; |
1407-1595 |
4.42e-18 |
|
Predicted N-acetyltransferase YhbS [General function prediction only];
Pssm-ID: 442387 [Multi-domain] Cd Length: 142 Bit Score: 82.44 E-value: 4.42e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1407 VRLATRDDVPRAVRTLAAAFAdypatrhtvdPDRHIERVTELQElfltrvGLDIGKVWVADDGAAVAvwttpesveaGAV 1486
Cdd:COG3153 1 IRPATPEDAEAIAALLRAAFG----------PGREAELVDRLRE------DPAAGLSLVAEDDGEIV----------GHV 54
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1487 FAEIgprmaelsgsrlaaqqqmegllAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRN 1566
Cdd:COG3153 55 ALSP----------------------VDIDGEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSL 112
|
170 180
....*....|....*....|....*....
gi 1559905467 1567 LPFYERLGFTVTADVEVPEGPRTWCMTRK 1595
Cdd:COG3153 113 LPFYERFGFRPAGELGLTLGPDEVFLAKE 141
|
|
| Acetyltransf_7 |
pfam13508 |
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions. |
1527-1577 |
1.71e-07 |
|
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
Pssm-ID: 463905 [Multi-domain] Cd Length: 84 Bit Score: 50.15 E-value: 1.71e-07
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|.
gi 1559905467 1527 VGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTV 1577
Cdd:pfam13508 34 LAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNRAAAFYEKLGFEE 84
|
|
| NAT_SF |
cd04301 |
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ... |
1511-1558 |
9.43e-04 |
|
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.
Pssm-ID: 173926 [Multi-domain] Cd Length: 65 Bit Score: 39.18 E-value: 9.43e-04
10 20 30 40
....*....|....*....|....*....|....*....|....*...
gi 1559905467 1511 LLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAF 1558
Cdd:cd04301 15 SLSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRL 62
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| cas_Csn1 |
TIGR01865 |
CRISPR subtype II/NMENI RNA-guided endonuclease Cas9/Csn1; CRISPR loci appear to be mobile ... |
4-1050 |
0e+00 |
|
CRISPR subtype II/NMENI RNA-guided endonuclease Cas9/Csn1; CRISPR loci appear to be mobile elements with a wide host range. This model represents a protein found only in CRISPR-containing species, near other CRISPR-associated proteins (cas), as part of the NMENI subtype of CRISPR/Cas locus. The species range so far for this protein is animal pathogens and commensals only.
Pssm-ID: 273840 Cd Length: 805 Bit Score: 899.48 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 4 KYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNtdrhsiKKNLIGALLFDSGETAE-ATRLKRTARRRYTRRKNRICYL 82
Cdd:TIGR01865 1 EYILGLDIGIASVGWAIVEDDYKVPAAKRLIDGG------VRNFTGAELPKTGETAAlDRRLARGARRRIRRRKHRLLRL 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 83 QEIFSNEMAKVDDSFFHRLEESFLVEEDKKHerhpifgnivdevayhekypTIYHLRKKLVDSTDKADLrlIYLALAHMI 162
Cdd:TIGR01865 75 QELFSREGSLTDFDFFSRLENSFLVEEDKRN--------------------TIYHLRKAALENKLKPDE--LYLALLHII 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 163 KFRGHFLIEgdlnpdnsdvdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfgnli 242
Cdd:TIGR01865 133 KHRGHFLIE----------------------------------------------------------------------- 141
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 243 alslgltpnfksnfdlaedaklqlskdtyDDDLDnllaqigdqyadlflaaknlsdaillsdilrVNTEITKAPLSASMI 322
Cdd:TIGR01865 142 -----------------------------GNDFD-------------------------------TANKETGALLSAVMI 161
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 323 KRYDEHHQDLTLLKALVRQQLPEKYKEIFFDqskngyagyidggasqeefykfikpilekmdgteellvklnreDLLRKQ 402
Cdd:TIGR01865 162 NRYLEHEADLRTLKELILKKFPKKYKEIFSE-------------------------------------------TFLRNQ 198
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 403 RTFDNGSIPHQIHLGELHAILRRQEDFYPFlkdnrekiEKILTFRIPYYVGPLARGNSRFAwmtrkseetitpwnfeeVV 482
Cdd:TIGR01865 199 RGFYNGSIPRQLLLEELEAIFRKQREYYPF--------IKLLTFRIPYYIGPLAEGKSEFA-----------------FV 253
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 483 DKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVK 562
Cdd:TIGR01865 254 DKPASAENFIEKMTGKCTYLPEEKRAPKHSLLAEKFTVLNELNNVRIIILEQGETKILSKEEKQELLDLLFKKKKLTYKK 333
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 563 QLKEDYFKKIECFDSVEISGVEDR---FNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY 639
Cdd:TIGR01865 334 LRKLLGLSEDAIFKGLRYEGLDNAekaFNISLKTYHKLRKALGDKDLLDNPKNPKDLDEIVKILTLYKDREMIKKRLELY 413
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 640 AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAqvsgqgds 719
Cdd:TIGR01865 414 KDVLNEEQVKKLVRLHFTGWGRLSLKALRGIRPLMEQGKRYDEAILELGGNRNFMQNINDSQLLPKINITKA-------- 485
|
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 720 lhehiANLAGSPAIKKGILQTVKVVDELVKVMGrhKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGS----QI 795
Cdd:TIGR01865 486 -----KDEILNPVVKRALLQARKVVNELVKKYG--PPDKIVIEMAREEQGTNFGKRNSKERYKKNEDKIKEFASalgkEI 558
|
810 820 830 840 850 860 870 880
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 796 LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRL---SDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS 872
Cdd:TIGR01865 559 LKEEPTENSSKNILKLRLYYQQNGKCMYTGKEIDIDDLfdlSYYEIDHILPQSRSFDDSISNKVLVLASENQEKGDQTPY 638
|
890 900 910 920 930 940 950 960
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 873 E-EVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDendklIR 951
Cdd:TIGR01865 639 EaEIVKKDSAFWNKFEAYVLISKRKSDKLTRAERGGLSDDDKAGFIDRNLNDTRYITRVVANYLKDRFNFHLK-----KR 713
|
970 980 990 1000 1010 1020 1030 1040
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 952 EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAkseqeigK 1031
Cdd:TIGR01865 714 KVKVVTLKGQLTSQLRKKWGLYKKREINNYHHAHDAYINAVSTNALVKKFSQLEPEFRYKEYHNFDGRKKKK-------S 786
|
1050
....*....|....*....
gi 1559905467 1032 ATAKYFFYSNIMNFFKTEI 1050
Cdd:TIGR01865 787 ATDKKVKFSNPMEFFKQKV 805
|
|
| Csn1 |
cd09643 |
CRISPR/Cas system-associated protein Cas9; CRISPR (Clustered Regularly Interspaced Short ... |
4-1049 |
0e+00 |
|
CRISPR/Cas system-associated protein Cas9; CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Very large protein containing McrA/HNH-nuclease related domain and a RuvC-like nuclease domain; signature gene for type II
Pssm-ID: 187774 [Multi-domain] Cd Length: 799 Bit Score: 885.22 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 4 KYSIGLDIGTNSVGWAVITDEYKVPSKKFKvlgntdrHSIKKNLIGALLFDSGETAE-ATRLKRTARRRYTRRKNRICYL 82
Cdd:cd09643 1 EYILGLDIGIASVGWAIVEDDYKVPAKKMI-------DCGVKIFTGAELFKTGETAAlDRRLARGARRRIRRRKHRLLRL 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 83 QEIFSNEMAKVDDSFFHRLEESFLveedkkherhpifgnivdevAYHEKYPTIYHLRKKLVDSTDKADLrlIYLALAHMI 162
Cdd:cd09643 74 QELFAREGSLTDFDFFSRLEDSFL--------------------EYHKNYPTIYHLRKAALENKLKPDE--LYLALLHII 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 163 KFRGHFLIEGDLNPDNsdvdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfgnli 242
Cdd:cd09643 132 KHRGHFLIEGDEDTTA---------------------------------------------------------------- 147
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 243 alslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlflaaknlsdaillsdilrvnTEITKAPLSASMI 322
Cdd:cd09643 148 -------------------------------------------------------------------DKETGALLSASMI 160
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 323 KRYDEHHQDLTLLKALVRQQLPEKYKEIFFDqskngyagyidggasqeefykfikpilekmdgteellvklnrEDLLRKQ 402
Cdd:cd09643 161 KRYDEHKADLRKLKELIKKEFFKKYKEIFGD------------------------------------------ETFLRNQ 198
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 403 RTFDNGSIPHQIHLGELHAILRRQEDFYPFlkdnrekiEKILTFRIPYYVGPLARGNSRFAWMTRKSEEtitpwnfeevv 482
Cdd:cd09643 199 RGFYNGSIPRQLLLEELEAIFRKQREYYPF--------EKILTFRIPYYIGPLAEGKSEFAWLTRPALS----------- 259
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 483 dkgasaQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEgMRKPAFLSGEQKKAIVDLLFKTNRKVTVK 562
Cdd:cd09643 260 ------EAFIEKMTGKCTYLPEEKRAPKHSLLAEKFTVLNELNNLRIIEE-QGETKILSKEEKQELLDLLFKKNKLTYKQ 332
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 563 QLKEDYFKKIECFDSVEISG--VEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYA 640
Cdd:cd09643 333 KRKLLGLKEEEIFKGLRYEGlkAEKNFNISLKTYHDLRKALGKEFLKDLELNEKILDEIVKILTLYKDREMIEKILELYK 412
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 641 HLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNfmQLIHDDSLTFKEDIQKAQVsgqgdsl 720
Cdd:cd09643 413 DLLNEEQLKKLLKRHFTGWGRLSLKALRGIRPLMEQGKRYDEAILELGGNHN--QKINSDELKFLPIIKKAQV------- 483
|
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 721 hehiANLAGSPAIKKGILQTVKVVDELVKVMGrhKPENIVIEMARENQtTQKGQKNSRERMKRIEEGIKELGS---QILK 797
Cdd:cd09643 484 ----KDEILNPVVKRALLQARKVVNELVKKYG--PPDKIVIEMARENG-TNKGTKNRKKRQKKNEDNIKEAASaleQKLK 556
|
810 820 830 840 850 860 870 880
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 798 EHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRL---SDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEE 874
Cdd:cd09643 557 ELPLDIKSKNILKLRLYYQQNGKCMYTGKEIDIDDLfdlSYYEIDHILPQSRSFDDSISNKVLVLASENQEKGDQTPYEE 636
|
890 900 910 920 930 940 950 960
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 875 VVKKMKNYWRQLLNAKLITQR---KFDNLtKAERgGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDendklIR 951
Cdd:cd09643 637 IVSKMSAFWNKLEAAKLISQRgdsKKDRL-LLEK-GISDDEKAGFIDRNLNDTRYITRVVANYLKDRFNFHLK-----KR 709
|
970 980 990 1000 1010 1020 1030 1040
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 952 EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLEsefVYGDYKVYDVRKMIAKSEQEIgk 1031
Cdd:cd09643 710 KVKVVTLKGQLTSQLRKKWGLYKKREINNYHHAHDAYINAVVTNALVKKFSQLE---RYKEYKRFDSEKGNKKTLDEN-- 784
|
1050
....*....|....*...
gi 1559905467 1032 ataKYFFYSNIMNFFKTE 1049
Cdd:cd09643 785 ---KKFFFANPMNFFKQE 799
|
|
| Cas9_REC |
pfam16592 |
REC lobe of CRISPR-associated endonuclease Cas9; The REC lobe of Cas9 - the CRISPR-associated ... |
181-710 |
0e+00 |
|
REC lobe of CRISPR-associated endonuclease Cas9; The REC lobe of Cas9 - the CRISPR-associated endonuclease Cas9 - includes the REC1 and REC2 domains. REC1 forms an elongated, alpha-helical structure consisting of 25 alpha helices and two beta-sheets, whereas REC2 inserted within REC1 adopts a six-helix bundle structure. The REC lobe and the NUC lobe of Cas9 fold to present a positively charged groove at their interface which accommodates the negatively charged sgRNA:target DNA heteroduplex. CRISPR (clustered regularly interspaced short palindromic repeat)-Cas system occurs naturally in bacteria as a defence against invasion by phages or other mobile genetic elements. Cas9 is targeted to specific genomic locations by sgRNAs or single guide RNAs, in order to complex with invading DNA in order to cleave it and render it inactive.
Pssm-ID: 435447 Cd Length: 539 Bit Score: 575.94 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 181 VDKLFIQLVQTYNQLFEENPINASGVDAKAILSA-RLSKSRRLENLIAQLPGEK-KNGLFGNLIALSLGLTPNFKSNFDL 258
Cdd:pfam16592 1 VEESFQDLLNILYEQLENLELETQNVEIEKILKKtKISKKAKLDELLALPPNEKnSKKIFAEILKLILGNKADFTKIFEL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 259 ------AEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL 332
Cdd:pfam16592 81 ekfveePKKIKLSFSDSNYDEKIEELENQLGDEKAEIILILKKIYDWVVLSDILTVSTDNGKAYLSEAMVNRYDKHKEDL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 333 TLLKALVRQQLPEKYKEIFFDQSKNGYAGYID----GGASQEEFYKFIKPILEKMDGTEE--LLVKLNREDLLRKQRTFD 406
Cdd:pfam16592 161 AQLKKVIKQNLSEKYNDMFRKEKKKGYSAYINgknnGKTSKEDFYKYIKKLINKVETSEAqyILSKIDNENFLPKQRTKS 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 407 NGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGA 486
Cdd:pfam16592 241 NGSIPYQVHLQELKKIIKNQAEYYPFLKENQEKILKLLTFRIPYYVGPLAEKKSKFAWMKRKEQGKIYPWNFEQKVDIDK 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 487 SAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEgmrkpaFLSGEQKKAIVDLLFKTNRKVTVKQLKE 566
Cdd:pfam16592 321 TAEAFITRMTNYCTYLPDEKVLPKNSLLYSKFTVLNELNKIKINGE------KISVELKQDIFNGLFKKNKKVTKKKLKD 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 567 DYFKKIECFDSVEISGV--EDRFNASLGTYHDLLKIIkdKDFLDNEENEDILEDIVLTLTLFEDREMIEERL-KTYAHLF 643
Cdd:pfam16592 395 WLVKEGYNFKAVEIKGFdkENNFNNSLTTYIDLAKIF--GDFLDNPDNEDIIEDIIYWLTLFEDRKILKRRLqKKYSNLL 472
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 644 DDKVMKQLKRRRYTGWGRLSRKLINGIRDKQS---GKTILDFLKSDgfaNRNFMQLIHDDSLTFKEDIQK 710
Cdd:pfam16592 473 TEKQIKQILKLKYKGWGRLSKELLNGIRGADRqgeIKTIIDLLWND---NRNLMQLINDERLSFKEEIEK 539
|
|
| Cas9 |
COG3513 |
CRISPR-Cas system type-II protein Cas9 [Defense mechanisms]; CRISPR-Cas system type-II protein ... |
3-1018 |
8.18e-128 |
|
CRISPR-Cas system type-II protein Cas9 [Defense mechanisms]; CRISPR-Cas system type-II protein Cas9 is part of the Pathway/BioSystem: CRISPR-Cas system
Pssm-ID: 442735 [Multi-domain] Cd Length: 812 Bit Score: 418.98 E-value: 8.18e-128
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 3 KKYSIGLDIGTNSVGWAVITDEYKVpskkfkvlgntdrHSIKKNLIGALLFDSGET-------AEATRLKRTARRRYTRR 75
Cdd:COG3513 2 DKYILGLDLGINSVGWAVLELDEDG-------------EPGEIIDAGVRIFDDGEDpksgeslAAARREARGARRRRRRR 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 76 KNRICYLQEIFSNEMakvddsffhrleesFLVEEDKKHERHPifgnivdevayhekYPTIYHLRKKLVDstDKADLRLIY 155
Cdd:COG3513 69 KHRLRRLKRLLVEEG--------------LLPADDAERKALL--------------PLNPYELRAKALD--EKLSPEELG 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 156 LALAHMIKFRGHfliegdLNPDNSDVDKLfiqlvqtynqlfeenpinasgvDAKAILSARLSKSRRLENLIAQLPGEkkn 235
Cdd:COG3513 119 RALFHLAQRRGF------KSNRKTDSKDN----------------------ESGKVKDAIKELRERLEAKGARTVGE--- 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 236 glfgnlialslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlFLAaknlsdaillsdilrvnteitka 315
Cdd:COG3513 168 ------------------------------------------------------YLY----------------------- 170
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 316 plsasmiKRYDEHHQdltllkalvrqqlpekykeiffdqskngyagyidggasqeefykfikpilekmdgteellvklnr 395
Cdd:COG3513 171 -------RRLQENGK----------------------------------------------------------------- 178
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 396 edlLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDN--REKIEKILTFRIPYYVGplargnsrfawmtrkseeti 473
Cdd:COG3513 179 ---VRNRKGDYDFYIPREDLEDEFEAIWAAQAEFGPALLTEelRDELLEIIFFQRPLKSG-------------------- 235
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 474 tpwnfeevvdkgasaqsfiERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGmRKPAFLSGEQKKAIVDLLF 553
Cdd:COG3513 236 -------------------KKLVGKCTFEPDEKRAPKASPLFQRFRILQKLNNLRIVDDG-GEERPLTLEERQKIIDLLE 295
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 554 KtNRKVTVKQLKEDYfkKIEcfDSVEISGVEDRFN-----ASLGTYHDLLKIIKDKDFldNEENEDILEDIVLTLTLFED 628
Cdd:COG3513 296 N-KKKLTFKKLRKLL--GLP--DGVIFKGFNYEDDdraklKGDKTYAKLAKIFGKAWL--NEFDPEILDDIVEALTLFKD 368
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 629 REMIEERLKTYAHLfDDKVMKQLKRRR-YTGWGRLSRKLINGIrdkqsgktiLDFLKSDgfanrnfmqlihddsLTFKED 707
Cdd:COG3513 369 DEELKEWLKKLYGL-DEEQAEALANLPlPDGYGNLSLKALRKI---------LPLLEEG---------------LDYDEA 423
|
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 708 IQKAQVSGQGDSLH--------EHIANLAGSPAIKKGILQTVKVVDELVKVMGrhKPENIVIEMARENQTTQKGQKNSRE 779
Cdd:COG3513 424 VKAAGYDHSSLEILdrlppigeEKRKGSIRNPVVHRALNQLRKVVNALIRKYG--KPDEIHIELARDLKKSKKERKEIQK 501
|
810 820 830 840 850 860 870 880
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 780 RMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSD--YDVDHIVPQSFLKDDSIDNKVL 857
Cdd:COG3513 502 RQRENEKAREKAREEIAEEGGGEPSRRDILKYRLWEEQNGRCPYTGKPISISDLLDgsVEIDHILPRSRTLDDSFNNKVL 581
|
890 900 910 920 930 940 950 960
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 858 TRSDKNRGKSDNVPSEEVVK----KMKNYWRQLLNAKLITQRKFDNLTKAERGglsELDKAGFIKRQLVETRQITKHVAQ 933
Cdd:COG3513 582 CLADANREKGNRTPYEALGGdeaeKWEEILARVENLKLIPQKKKKRFLKKELD---RDDDEGFIARQLNDTRYISRLAAE 658
|
970 980 990 1000 1010 1020 1030 1040
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 934 ILDSRMNTKYdendklIREVKVITLKSKLVSDFRKDFQFYKV-------REINNYHHAHDAYLNAVVGTALIKKYPKLES 1006
Cdd:COG3513 659 YLKSLYPFED------KGKRKVRVVPGQLTAMLRRAWGLNKIlsddgekNRDDHRHHAIDALVIACTTQGLLQRLAKASR 732
|
1050
....*....|..
gi 1559905467 1007 EFVYGDYKVYDV 1018
Cdd:COG3513 733 EREDAEKAEEHF 744
|
|
| Cas9_PI |
pfam16595 |
PAM-interacting domain of CRISPR-associated endonuclease Cas9; Cas9_PI is a family found at ... |
1102-1358 |
5.82e-47 |
|
PAM-interacting domain of CRISPR-associated endonuclease Cas9; Cas9_PI is a family found at the C-terminal of bacterial type II CRISPR system Cas9 endonuclease. This domain adopts a novel protein fold that is unique to the Cas9 family. It is positioned in the structure-DNA-complex to recognize the PAM sequence on the non-complementary DNA strand of the crRNA. PAM sequence is protospacer-adjacent motifs on DNA. See family CRISPR-DR2, Rfam:RF01315. Cas9 carries two nuclease domains, HNH and RuvC, which cleave the DNA strands that are complementary and non-complementary to the 20 nucleotide guide sequence in crRNAs, respectively.
Pssm-ID: 435449 Cd Length: 264 Bit Score: 169.81 E-value: 5.82e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1102 TGGFSKESILP--KRNSDKLIARKKD---WDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLksvkeLLGITIMERSSFEK 1176
Cdd:pfam16595 1 KGGLFNQTILPahKKKGKGLIPLKKDergLDVEKYGGYSSLTAAYFSLVEYTGKKGKRKRT-----IEGVPLYLAAKIEE 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1177 NPI--DFLEAKGYKEVKKDLIIKLPKYSLFElENGRKRMLASAGE---LQKGNELALPSKYVNFLYLASHYEKLKGSPED 1251
Cdd:pfam16595 76 NKDllEYLEEKLGLKEPKIILPKIKKNSLIK-IDGFRMLLTGKTEnrlLKNAVQLVLSNDDEKYIKKIEKFVKKNKDDII 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1252 NEQKQLFVEQHKHYLDEIIEQISEFSKrVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA-PAAFKYFDTT 1330
Cdd:pfam16595 155 EEKDGLTEEKNIKLYDELLDKMKNTIY-YKRPSNQGEKLEKLKEKFIKLSLEEKCKVLIEILKLTHANPtSADLKLIGGS 233
|
250 260 270
....*....|....*....|....*....|.
gi 1559905467 1331 IDRKRYTSTKEVLDA---TLIHQSITGLYET 1358
Cdd:pfam16595 234 KHAGRIKISNNISKAsniKLINQSVTGLYEK 264
|
|
| yhbS |
COG3153 |
Predicted N-acetyltransferase YhbS [General function prediction only]; |
1407-1595 |
4.42e-18 |
|
Predicted N-acetyltransferase YhbS [General function prediction only];
Pssm-ID: 442387 [Multi-domain] Cd Length: 142 Bit Score: 82.44 E-value: 4.42e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1407 VRLATRDDVPRAVRTLAAAFAdypatrhtvdPDRHIERVTELQElfltrvGLDIGKVWVADDGAAVAvwttpesveaGAV 1486
Cdd:COG3153 1 IRPATPEDAEAIAALLRAAFG----------PGREAELVDRLRE------DPAAGLSLVAEDDGEIV----------GHV 54
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1487 FAEIgprmaelsgsrlaaqqqmegllAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRN 1566
Cdd:COG3153 55 ALSP----------------------VDIDGEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSL 112
|
170 180
....*....|....*....|....*....
gi 1559905467 1567 LPFYERLGFTVTADVEVPEGPRTWCMTRK 1595
Cdd:COG3153 113 LPFYERFGFRPAGELGLTLGPDEVFLAKE 141
|
|
| HNH_4 |
pfam13395 |
HNH endonuclease; This HNH nuclease domain is found in CRISPR-related proteins. |
821-871 |
1.83e-14 |
|
HNH endonuclease; This HNH nuclease domain is found in CRISPR-related proteins.
Pssm-ID: 433172 [Multi-domain] Cd Length: 55 Bit Score: 69.19 E-value: 1.83e-14
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....
gi 1559905467 821 DMYVDQELDINRLSD---YDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP 871
Cdd:pfam13395 1 CPYTGEQISIDDLFSeknYDIDHILPYSRSFDDSFSNKVLVLRSANQEKGNRTP 54
|
|
| RimI |
COG0456 |
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ... |
1511-1595 |
5.03e-11 |
|
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];
Pssm-ID: 440224 [Multi-domain] Cd Length: 92 Bit Score: 60.44 E-value: 5.03e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1511 LLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVP-AFLETSA--PRNLPFYERLGFTVTADVEVPEGP 1587
Cdd:COG0456 3 ALLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARrLRLEVREdnEAAIALYEKLGFEEVGERPNYYGD 82
|
....*...
gi 1559905467 1588 RTWCMTRK 1595
Cdd:COG0456 83 DALVMEKE 90
|
|
| MnaT |
COG1247 |
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism]; |
1406-1582 |
9.14e-10 |
|
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
Pssm-ID: 440860 [Multi-domain] Cd Length: 163 Bit Score: 59.24 E-value: 9.14e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1406 TVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRhiervtELQELFLTRVGLDIGKVWVA-DDGAAVAvwttpesveag 1484
Cdd:COG1247 3 TIRPATPEDAPAIAAIYNEAIAEGTATFETEPPSE------EEREAWFAAILAPGRPVLVAeEDGEVVG----------- 65
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1485 avFAEIGPRmaelsgsrlaaqqqmegllaPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP 1564
Cdd:COG1247 66 --FASLGPF--------------------RPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLA 123
|
170 180
....*....|....*....|.
gi 1559905467 1565 RN---LPFYERLGFTVTADVE 1582
Cdd:COG1247 124 DNeasIALYEKLGFEEVGTLP 144
|
|
| ArgA |
COG1246 |
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ... |
1406-1591 |
1.02e-08 |
|
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis
Pssm-ID: 440859 [Multi-domain] Cd Length: 132 Bit Score: 55.38 E-value: 1.02e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1406 TVRLATRDDVPrAVRTLAAAFADYPatrhtvdpdrhiervtelqelfltrvglDIGKVWVADDGAAVAvwttpesveAGA 1485
Cdd:COG1246 2 TIRPATPDDVP-AILELIRPYALEE----------------------------EIGEFWVAEEDGEIV---------GCA 43
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1486 VFAEIGPRMAELsgsrlaaqqqmegllaphrpkepawflATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPA-FLETSaP 1564
Cdd:COG1246 44 ALHPLDEDLAEL---------------------------RSLAVHPDYRGRGIGRRLLEALLAEARELGLKRlFLLTT-S 95
|
170 180
....*....|....*....|....*..
gi 1559905467 1565 RNLPFYERLGFTVTADVEVPEGPRTWC 1591
Cdd:COG1246 96 AAIHFYEKLGFEEIDKEDLPYAKVWQR 122
|
|
| COG3393 |
COG3393 |
Predicted acetyltransferase, GNAT family [General function prediction only]; |
1527-1580 |
1.12e-07 |
|
Predicted acetyltransferase, GNAT family [General function prediction only];
Pssm-ID: 442620 [Multi-domain] Cd Length: 86 Bit Score: 50.68 E-value: 1.12e-07
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*..
gi 1559905467 1527 VGVSPDHQGKGLGSAVVLPGVEAAERAGVP-AFLETSA--PRNLPFYERLGFTVTAD 1580
Cdd:COG3393 21 VYTHPEYRGRGLASALVAALAREALARGARtPFLYVDAdnPAARRLYERLGFRPVGE 77
|
|
| Acetyltransf_7 |
pfam13508 |
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions. |
1527-1577 |
1.71e-07 |
|
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
Pssm-ID: 463905 [Multi-domain] Cd Length: 84 Bit Score: 50.15 E-value: 1.71e-07
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|.
gi 1559905467 1527 VGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTV 1577
Cdd:pfam13508 34 LAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNRAAAFYEKLGFEE 84
|
|
| Acetyltransf_1 |
pfam00583 |
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ... |
1510-1575 |
1.94e-07 |
|
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.
Pssm-ID: 395465 [Multi-domain] Cd Length: 116 Bit Score: 50.98 E-value: 1.94e-07
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1559905467 1510 GLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPA-FLETSAP--RNLPFYERLGF 1575
Cdd:pfam00583 48 ASLSIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERiFLEVAADnlAAIALYEKLGF 116
|
|
| PhnO |
COG0454 |
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ... |
1523-1587 |
2.14e-06 |
|
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];
Pssm-ID: 440222 [Multi-domain] Cd Length: 136 Bit Score: 48.51 E-value: 2.14e-06
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1559905467 1523 FLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAF-LETSA--PRNLPFYERLGFTVTADVEVPEGP 1587
Cdd:COG0454 60 ELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALeLDTLDgnPAAIRFYERLGFKEIERYVAYVGG 127
|
|
| ElaA |
COG2153 |
Predicted N-acyltransferase, GNAT family [General function prediction only]; |
1527-1581 |
3.98e-05 |
|
Predicted N-acyltransferase, GNAT family [General function prediction only];
Pssm-ID: 441756 [Multi-domain] Cd Length: 134 Bit Score: 44.79 E-value: 3.98e-05
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*.
gi 1559905467 1527 VGVSPDHQGKGLGSAVVLPGVEAAERAGVPAfLETSA-PRNLPFYERLGFTVTADV 1581
Cdd:COG2153 64 VAVLPEYRGQGLGRALMEAAIEEARERGARR-IVLSAqAHAVGFYEKLGFVPVGEE 118
|
|
| NAT_SF |
cd04301 |
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ... |
1511-1558 |
9.43e-04 |
|
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.
Pssm-ID: 173926 [Multi-domain] Cd Length: 65 Bit Score: 39.18 E-value: 9.43e-04
10 20 30 40
....*....|....*....|....*....|....*....|....*...
gi 1559905467 1511 LLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAF 1558
Cdd:cd04301 15 SLSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRL 62
|
|
| Eis |
COG4552 |
Predicted acetyltransferase [General function prediction only]; |
1406-1584 |
2.52e-03 |
|
Predicted acetyltransferase [General function prediction only];
Pssm-ID: 443616 [Multi-domain] Cd Length: 393 Bit Score: 42.19 E-value: 2.52e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1406 TVRLATRDDVPRAVRTLAAAFADYPatrhtvdPDRHIERVTELqelfltrvgLDIGKVWVA-DDGAAVAVwttpesveaG 1484
Cdd:COG4552 2 EIRPLTEDDLDAFARLLAYAFGPEP-------DDEELEAYRPL---------LEPGRVLGVfDDGELVGT---------L 56
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1485 AVFaeigPRMAELSGSRLaaqqqmegllaphrpkePAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVP-AFLETSA 1563
Cdd:COG4552 57 ALY----PFTLNVGGARV-----------------PMAGITGVAVAPEHRRRGVARALLREALAELRERGQPlSALYPFE 115
|
170 180
....*....|....*....|....
gi 1559905467 1564 PrnlPFYERLGFTVTAD---VEVP 1584
Cdd:COG4552 116 P---GFYRRFGYELAGDrrrYTIP 136
|
|
| Acetyltransf_10 |
pfam13673 |
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ... |
1529-1586 |
3.15e-03 |
|
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.
Pssm-ID: 463953 [Multi-domain] Cd Length: 128 Bit Score: 39.18 E-value: 3.15e-03
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 1529 VSPDHQGKGLGSAVVLPGVEAAERAG--VPAFLETSAPRNLPFYERLGFTVTADVEVPEG 1586
Cdd:pfam13673 59 VDPDYQGQGIGKALLEAVEDYAEKDGikLSELTVNASPYAVPFYEKLGFRATGPEQEFNG 118
|
|
| COG3472 |
COG3472 |
Uncharacterized conserved protein domain, often C-terminal to DUF262 [Function unknown]; |
811-888 |
3.80e-03 |
|
Uncharacterized conserved protein domain, often C-terminal to DUF262 [Function unknown];
Pssm-ID: 442695 [Multi-domain] Cd Length: 566 Bit Score: 41.91 E-value: 3.80e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1559905467 811 LYLYYLQNG-RDMYVDQELDINRLSDYDVD--HIVPQSFLKD--------DSIDNKVLTRSDKNRGKSDNVPSEevvkkm 879
Cdd:COG3472 432 ILALLAKLGaRDFLSGQKIDLSNLFDNKLEihHIFPKAYLKKqgisrslyNSIANRTPLSARTNRKIGDKAPSE------ 505
|
....*....
gi 1559905467 880 knYWRQLLN 888
Cdd:COG3472 506 --YLAELEE 512
|
|
| FR47 |
pfam08445 |
FR47-like protein; The members of this family are similar to the C-terminal region of the D. ... |
1523-1577 |
9.16e-03 |
|
FR47-like protein; The members of this family are similar to the C-terminal region of the D. melanogaster hypothetical protein FR47. This protein has been found to consist of two N-acyltransferase-like domains swapped with the C-terminal strands.
Pssm-ID: 117022 [Multi-domain] Cd Length: 86 Bit Score: 36.92 E-value: 9.16e-03
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*...
gi 1559905467 1523 FLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETsAPRNLP---FYERLGFTV 1577
Cdd:pfam08445 23 ELGALQTLPEHRRRGLGSRLVAALARGIAERGITPFAVV-VAGNTPsrrLYEKLGFRK 79
|
|
| |
