NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|2045760702|gb|QVX24275|]
View 

Ribosomal protein S30 [Vector pSC101_rK-sacB_selection-construct]

Protein Classification

30S ribosomal protein S12( domain architecture ID 17564814)

30S ribosomal protein S12 assists in rRNA folding and the binding of other proteins during small subunit assembly

Gene Ontology:  GO:0006412|GO:0019843|GO:0003735
PubMed:  24524803

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
RpsL COG0048
Ribosomal protein S12 [Translation, ribosomal structure and biogenesis]; Ribosomal protein S12 ...
 1-124 3.51e-89

Ribosomal protein S12 [Translation, ribosomal structure and biogenesis]; Ribosomal protein S12 is part of the Pathway/BioSystem: Ribosome 30S subunit


:

Pssm-ID: 439818  Cd Length: 124  Bit Score: 253.97  E-value: 3.51e-89
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702   1 MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVI 80
Cdd:COG0048     1 MPTINQLVRKGRKKKKKKSKSPALKGCPQKRGVCTRVYTTTPKKPNSALRKVARVRLSNGIEVTAYIPGEGHNLQEHSVV 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 2045760702  81 LIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA 124
Cdd:COG0048    81 LVRGGRVKDLPGVRYHIVRGALDTAGVKGRKQSRSKYGAKKPKA 124
 
Name Accession Description Interval E-value
RpsL COG0048
Ribosomal protein S12 [Translation, ribosomal structure and biogenesis]; Ribosomal protein S12 ...
 1-124 3.51e-89

Ribosomal protein S12 [Translation, ribosomal structure and biogenesis]; Ribosomal protein S12 is part of the Pathway/BioSystem: Ribosome 30S subunit


Pssm-ID: 439818  Cd Length: 124  Bit Score: 253.97  E-value: 3.51e-89
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702   1 MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVI 80
Cdd:COG0048     1 MPTINQLVRKGRKKKKKKSKSPALKGCPQKRGVCTRVYTTTPKKPNSALRKVARVRLSNGIEVTAYIPGEGHNLQEHSVV 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 2045760702  81 LIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA 124
Cdd:COG0048    81 LVRGGRVKDLPGVRYHIVRGALDTAGVKGRKQSRSKYGAKKPKA 124
rpsL_bact TIGR00981
ribosomal protein S12, bacterial/organelle; This model recognizes ribosomal protein S12 of ...
 1-124 4.31e-80

ribosomal protein S12, bacterial/organelle; This model recognizes ribosomal protein S12 of Bacteria, mitochondria, and chloroplasts. The homologous ribosomal proteins of Archaea and Eukarya, termed S23 in Eukarya and S12 or S23 in Archaea, score below the trusted cutoff. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 130054  Cd Length: 124  Bit Score: 231.21  E-value: 4.31e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702   1 MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVI 80
Cdd:TIGR00981   1 MPTINQLIRKGRKKKKKKSKSPALEACPQKRGVCTRVYTTTPKKPNSALRKVARVRLTNGFEVTAYIPGEGHNLQEHSVV 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 2045760702  81 LIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA 124
Cdd:TIGR00981  81 LIRGGRVKDLPGVRYHIVRGALDTAGVKNRKQGRSKYGAKRPKA 124
rps12 CHL00051
ribosomal protein S12
 1-123 1.74e-74

ribosomal protein S12


Pssm-ID: 176992  Cd Length: 123  Bit Score: 216.82  E-value: 1.74e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702   1 MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVI 80
Cdd:CHL00051    1 MPTIKQLIRNTRQPIRNKTKSPALRGCPQRRGTCTRVYTITPKKPNSALRKVARVRLTSGFEITAYIPGIGHNLQEHSVV 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2045760702  81 LIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPK 123
Cdd:CHL00051   81 LVRGGRVKDLPGVRYHIVRGTLDAVGVKDRQQGRSKYGVKKPK 123
Ribosomal_S12 cd03368
S12-like family, 30S ribosomal protein S12 subfamily; S12 is located at the interface of the ...
 3-110 1.06e-66

S12-like family, 30S ribosomal protein S12 subfamily; S12 is located at the interface of the large and small ribosomal subunits of prokaryotes, chloroplasts and mitochondria, where it plays an important role in both tRNA and ribosomal subunit interactions. S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as a control element for the rRNA- and tRNA-driven movements of translocation. Antibiotics such as streptomycin bind S12 and cause the ribosome to misread the genetic code.


Pssm-ID: 239466  Cd Length: 108  Bit Score: 196.59  E-value: 1.06e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702   3 TVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILI 82
Cdd:cd03368     1 TINQLIRKGRKKKKKKSKSPALEGCPQKKGVCLKVYTTTPKKPNSALRKVARVRLSNGKEVTAYIPGEGHNLQEHSVVLV 80

                          90       100
                  ....*....|....*....|....*...
gi 2045760702  83 RGGRVKDLPGVRYHTVRGALDCSGVKDR 110
Cdd:cd03368    81 RGGRVKDLPGVRYHIVRGVLDLAGVKNR 108
Ribosom_S12_S23 pfam00164
Ribosomal protein S12/S23; This protein is known as S12 in bacteria and archaea and S23 in ...
 12-123 7.40e-46

Ribosomal protein S12/S23; This protein is known as S12 in bacteria and archaea and S23 in eukaryotes.


Pssm-ID: 395112  Cd Length: 114  Bit Score: 144.13  E-value: 7.40e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702  12 RARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLT-NGFEVTSYIGGEG--HNLQEHSVILIRGGR-- 86
Cdd:pfam00164   1 RRLLGLKTKSPPLEGAPQARGVVLEKVTVEPKQPNSALRKCVRVRLIkNGKEVTAFIPGEGaiHFLQEHDEVLIRGGGgp 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 2045760702  87 ---VKDLPGVRYHTVRgaldCSGVKdrKQARSKYGVKRPK 123
Cdd:pfam00164  81 ghaVGDLPGVRYKVVR----VAGVS--LQALSKYGAEKPK 114
 
Name Accession Description Interval E-value
RpsL COG0048
Ribosomal protein S12 [Translation, ribosomal structure and biogenesis]; Ribosomal protein S12 ...
 1-124 3.51e-89

Ribosomal protein S12 [Translation, ribosomal structure and biogenesis]; Ribosomal protein S12 is part of the Pathway/BioSystem: Ribosome 30S subunit


Pssm-ID: 439818  Cd Length: 124  Bit Score: 253.97  E-value: 3.51e-89
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702   1 MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVI 80
Cdd:COG0048     1 MPTINQLVRKGRKKKKKKSKSPALKGCPQKRGVCTRVYTTTPKKPNSALRKVARVRLSNGIEVTAYIPGEGHNLQEHSVV 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 2045760702  81 LIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA 124
Cdd:COG0048    81 LVRGGRVKDLPGVRYHIVRGALDTAGVKGRKQSRSKYGAKKPKA 124
rpsL_bact TIGR00981
ribosomal protein S12, bacterial/organelle; This model recognizes ribosomal protein S12 of ...
 1-124 4.31e-80

ribosomal protein S12, bacterial/organelle; This model recognizes ribosomal protein S12 of Bacteria, mitochondria, and chloroplasts. The homologous ribosomal proteins of Archaea and Eukarya, termed S23 in Eukarya and S12 or S23 in Archaea, score below the trusted cutoff. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 130054  Cd Length: 124  Bit Score: 231.21  E-value: 4.31e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702   1 MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVI 80
Cdd:TIGR00981   1 MPTINQLIRKGRKKKKKKSKSPALEACPQKRGVCTRVYTTTPKKPNSALRKVARVRLTNGFEVTAYIPGEGHNLQEHSVV 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 2045760702  81 LIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA 124
Cdd:TIGR00981  81 LIRGGRVKDLPGVRYHIVRGALDTAGVKNRKQGRSKYGAKRPKA 124
rps12 CHL00051
ribosomal protein S12
 1-123 1.74e-74

ribosomal protein S12


Pssm-ID: 176992  Cd Length: 123  Bit Score: 216.82  E-value: 1.74e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702   1 MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVI 80
Cdd:CHL00051    1 MPTIKQLIRNTRQPIRNKTKSPALRGCPQRRGTCTRVYTITPKKPNSALRKVARVRLTSGFEITAYIPGIGHNLQEHSVV 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2045760702  81 LIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPK 123
Cdd:CHL00051   81 LVRGGRVKDLPGVRYHIVRGTLDAVGVKDRQQGRSKYGVKKPK 123
Ribosomal_S12 cd03368
S12-like family, 30S ribosomal protein S12 subfamily; S12 is located at the interface of the ...
 3-110 1.06e-66

S12-like family, 30S ribosomal protein S12 subfamily; S12 is located at the interface of the large and small ribosomal subunits of prokaryotes, chloroplasts and mitochondria, where it plays an important role in both tRNA and ribosomal subunit interactions. S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as a control element for the rRNA- and tRNA-driven movements of translocation. Antibiotics such as streptomycin bind S12 and cause the ribosome to misread the genetic code.


Pssm-ID: 239466  Cd Length: 108  Bit Score: 196.59  E-value: 1.06e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702   3 TVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILI 82
Cdd:cd03368     1 TINQLIRKGRKKKKKKSKSPALEGCPQKKGVCLKVYTTTPKKPNSALRKVARVRLSNGKEVTAYIPGEGHNLQEHSVVLV 80

                          90       100
                  ....*....|....*....|....*...
gi 2045760702  83 RGGRVKDLPGVRYHTVRGALDCSGVKDR 110
Cdd:cd03368    81 RGGRVKDLPGVRYHIVRGVLDLAGVKNR 108
Ribosom_S12_S23 pfam00164
Ribosomal protein S12/S23; This protein is known as S12 in bacteria and archaea and S23 in ...
 12-123 7.40e-46

Ribosomal protein S12/S23; This protein is known as S12 in bacteria and archaea and S23 in eukaryotes.


Pssm-ID: 395112  Cd Length: 114  Bit Score: 144.13  E-value: 7.40e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702  12 RARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLT-NGFEVTSYIGGEG--HNLQEHSVILIRGGR-- 86
Cdd:pfam00164   1 RRLLGLKTKSPPLEGAPQARGVVLEKVTVEPKQPNSALRKCVRVRLIkNGKEVTAFIPGEGaiHFLQEHDEVLIRGGGgp 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 2045760702  87 ---VKDLPGVRYHTVRgaldCSGVKdrKQARSKYGVKRPK 123
Cdd:pfam00164  81 ghaVGDLPGVRYKVVR----VAGVS--LQALSKYGAEKPK 114
PTZ00115 PTZ00115
40S ribosomal protein S12; Provisional
 24-120 3.96e-40

40S ribosomal protein S12; Provisional


Pssm-ID: 240276  Cd Length: 290  Bit Score: 134.97  E-value: 3.96e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702  24 LEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGALD 103
Cdd:PTZ00115  120 LEGAPQKKGICVKVRVQTPRKPNSGLRKVARVRLSTGRTVTVYIPGIGHNLNTHSVVLVRGGRCKDVPGCNYKAVRGVYD 199

                          90
                  ....*....|....*..
gi 2045760702 104 CSGVKDRKQARSKYGVK 120
Cdd:PTZ00115  200 LLPVKNRARSRSKYGVK 216
Ribosomal_S12_like cd00319
Ribosomal protein S12-like family; composed of prokaryotic 30S ribosomal protein S12, ...
 31-100 3.43e-14

Ribosomal protein S12-like family; composed of prokaryotic 30S ribosomal protein S12, eukaryotic 40S ribosomal protein S23 and similar proteins. S12 and S23 are located at the interface of the large and small ribosomal subunits, adjacent to the decoding center. They play an important role in translocation during the peptide elongation step of protein synthesis. They are also involved in important RNA and protein interactions. Ribosomal protein S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as a control element for the rRNA- and tRNA-driven movements of translocation. S23 interacts with domain III of the eukaryotic elongation factor 2 (eEF2), which catalyzes translocation. Mutations in S12 and S23 have been found to affect translational accuracy. Antibiotics such as streptomycin may also bind S12/S23 and cause the ribosome to misread the genetic code.


Pssm-ID: 238196  Cd Length: 95  Bit Score: 63.19  E-value: 3.43e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702  31 RGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRG 100
Cdd:cd00319    16 RGVCTVVRTVTPKKPNSALRKVAKVRLTSGYEVTAYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRG 85
Ribosomal_S23 cd03367
S12-like family, 40S ribosomal protein S23 subfamily; S23 is located at the interface of the ...
 22-99 1.04e-08

S12-like family, 40S ribosomal protein S23 subfamily; S23 is located at the interface of the large and small ribosomal subunits of eukaryotes, adjacent to the decoding center. It interacts with domain III of the eukaryotic elongation factor 2 (eEF2), which catalyzes the translocation of the growing peptidyl-tRNA to the P site to make room for the next aminoacyl-tRNA at the A (acceptor) site. Through its interaction with eEF2, S23 may play an important role in translocation. Also members of this subfamily are the archaeal 30S ribosomal S12 proteins. Prokaryotic S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as control element for the rRNA- and tRNA-driven movements of translocation. S12 and S23 are also implicated in translation accuracy. Antibiotics such as streptomycin bind S12/S23 and cause the ribosome to misread the genetic code.


Pssm-ID: 239465  Cd Length: 115  Bit Score: 49.58  E-value: 1.04e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702  22 PALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLT-NGFEVTSYIGGEG--HNLQEHSVILI-----RGGRVKDLPGV 93
Cdd:cd03367    14 DPLGGAPHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIkNGKKITAFVPGDGclNFIDENDEVLVagfgrKGRAVGDIPGV 93


                  ....*.
gi 2045760702  94 RYHTVR 99
Cdd:cd03367    94 RFKVVK 99
rps12P PRK04211
30S ribosomal protein S12P; Reviewed
 24-95 3.41e-08

30S ribosomal protein S12P; Reviewed


Pssm-ID: 235257  Cd Length: 145  Bit Score: 48.79  E-value: 3.41e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2045760702  24 LEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLT-NGFEVTSYIGGEG--HNLQEHSVILIRG-----GRVK-DLPGVR 94
Cdd:PRK04211   42 LEGAPMARGIVLEKVGVEAKQPNSAIRKCVRVQLIkNGKQVTAFCPGDGaiNFIDEHDEVVIEGiggpkGRSMgDIPGVR 121


                  .
gi 2045760702  95 Y 95
Cdd:PRK04211  122 Y 122
PTZ00067 PTZ00067
40S ribosomal S23; Provisional
 43-99 8.43e-06

40S ribosomal S23; Provisional


Pssm-ID: 185422  Cd Length: 143  Bit Score: 42.42  E-value: 8.43e-06
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2045760702  43 KKPNSALRKVCRVRLT-NGFEVTSYIGGEG--HNLQEHSVILIRG-GR----VKDLPGVRYHTVR 99
Cdd:PTZ00067   60 KQPNSAIRKCVRVQLIkNGKKITAFVPNDGclNFINENDEVLVSGfGRsghaVGDIPGVRFKVVK 124
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH