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Conserved domains on  [gi|1723593444|emb|VHO00141|]
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Resuscitation-promoting factor RpfA [Lawsonella clevelandensis]

Protein Classification

transglycosylase family protein( domain architecture ID 10535629)

transglycosylase family protein has peptidoglycan (PG) hydrolytic activity, similar to Mycobacterium tuberculosis Rpf resuscitation-promoting factors and Staphylococcus aureus transglycosylase SceD

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Transglycosylas pfam06737
Transglycosylase-like domain; This family of proteins are very likely to act as ...
 56-130 3.97e-43

Transglycosylase-like domain; This family of proteins are very likely to act as transglycosylase enzymes related to pfam00062 and pfam01464. These other families are weakly matched by this family, and include the known active site residues.


:

Pssm-ID: 399604 [Multi-domain]  Cd Length: 75  Bit Score: 143.71  E-value: 3.97e-43
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1723593444  56 PNSDWDRLAQCESGGNWHINTGNGYYGGLQFSQGTWAAHGGTNYAPSADLATRDQQIAVAEHVLATQGWGAWPAC 130
Cdd:pfam06737   1 DNSNWDAIAQCESGGNWAINTGNGYYGGLQFSQSTWDAYGGLGYAPSAAQASREQQIAVAEKLLATQGAGAWPTC 75
 
Name Accession Description Interval E-value
Transglycosylas pfam06737
Transglycosylase-like domain; This family of proteins are very likely to act as ...
 56-130 3.97e-43

Transglycosylase-like domain; This family of proteins are very likely to act as transglycosylase enzymes related to pfam00062 and pfam01464. These other families are weakly matched by this family, and include the known active site residues.


Pssm-ID: 399604 [Multi-domain]  Cd Length: 75  Bit Score: 143.71  E-value: 3.97e-43
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1723593444  56 PNSDWDRLAQCESGGNWHINTGNGYYGGLQFSQGTWAAHGGTNYAPSADLATRDQQIAVAEHVLATQGWGAWPAC 130
Cdd:pfam06737   1 DNSNWDAIAQCESGGNWAINTGNGYYGGLQFSQSTWDAYGGLGYAPSAAQASREQQIAVAEKLLATQGAGAWPTC 75
RPF cd13925
core lysozyme-like domain of resuscitation-promoting factor proteins; Resuscitation-promoting ...
 58-130 2.92e-32

core lysozyme-like domain of resuscitation-promoting factor proteins; Resuscitation-promoting factor (RPF) proteins, found in various (G+C)-rich Gram-positive bacteria, act to reactivate cultures from stationary phase. This protein shares elements of the structural core of lysozyme and related proteins. Furthermore, it shares a conserved active site glutamate which is required for activity, and has a polysaccharide binding cleft that corresponds to the peptidoglycan binding cleft of lysozyme. Muralytic activity of Rpf in Micrococcus luteus correlates with resuscitation, supporting a mechanism dependent on cleavage of peptidoglycan by RPF.


Pssm-ID: 381607 [Multi-domain]  Cd Length: 71  Bit Score: 115.17  E-value: 2.92e-32
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1723593444  58 SDWDRLAQCESGGNWH-INTGNGYYGGLQFSQGTWAAHGGTNYapsADLATRDQQIAVAEHVLATQGWGAWPAC 130
Cdd:cd13925     1 SQWDAIAQCESGGNWNaVNTGNGYYGGLQFLQGTWKSVGGLGY---PDDATRAEQITRAERLYARQGAGAWPCC 71
 
Name Accession Description Interval E-value
Transglycosylas pfam06737
Transglycosylase-like domain; This family of proteins are very likely to act as ...
 56-130 3.97e-43

Transglycosylase-like domain; This family of proteins are very likely to act as transglycosylase enzymes related to pfam00062 and pfam01464. These other families are weakly matched by this family, and include the known active site residues.


Pssm-ID: 399604 [Multi-domain]  Cd Length: 75  Bit Score: 143.71  E-value: 3.97e-43
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1723593444  56 PNSDWDRLAQCESGGNWHINTGNGYYGGLQFSQGTWAAHGGTNYAPSADLATRDQQIAVAEHVLATQGWGAWPAC 130
Cdd:pfam06737   1 DNSNWDAIAQCESGGNWAINTGNGYYGGLQFSQSTWDAYGGLGYAPSAAQASREQQIAVAEKLLATQGAGAWPTC 75
RPF cd13925
core lysozyme-like domain of resuscitation-promoting factor proteins; Resuscitation-promoting ...
 58-130 2.92e-32

core lysozyme-like domain of resuscitation-promoting factor proteins; Resuscitation-promoting factor (RPF) proteins, found in various (G+C)-rich Gram-positive bacteria, act to reactivate cultures from stationary phase. This protein shares elements of the structural core of lysozyme and related proteins. Furthermore, it shares a conserved active site glutamate which is required for activity, and has a polysaccharide binding cleft that corresponds to the peptidoglycan binding cleft of lysozyme. Muralytic activity of Rpf in Micrococcus luteus correlates with resuscitation, supporting a mechanism dependent on cleavage of peptidoglycan by RPF.


Pssm-ID: 381607 [Multi-domain]  Cd Length: 71  Bit Score: 115.17  E-value: 2.92e-32
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1723593444  58 SDWDRLAQCESGGNWH-INTGNGYYGGLQFSQGTWAAHGGTNYapsADLATRDQQIAVAEHVLATQGWGAWPAC 130
Cdd:cd13925     1 SQWDAIAQCESGGNWNaVNTGNGYYGGLQFLQGTWKSVGGLGY---PDDATRAEQITRAERLYARQGAGAWPCC 71
Lyz-like cd00442
lysozyme-like domains; This family contains several members, including soluble lytic ...
 60-115 3.21e-07

lysozyme-like domains; This family contains several members, including soluble lytic transglycosylases (SLT), goose egg-white lysozymes (GEWL), hen egg-white lysozymes (HEWL), chitinases, bacteriophage lambda lysozymes, endolysins, autolysins, chitosanases, and pesticin. Typical members are involved in the hydrolysis of beta-1,4- linked polysaccharides.


Pssm-ID: 381596 [Multi-domain]  Cd Length: 59  Bit Score: 46.63  E-value: 3.21e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1723593444  60 WDRLAQCESGGNWHIN--TGNGYYGGLQFSQGTWAAHGGTNyapSADLATRDQQIAVA 115
Cdd:cd00442     2 LAAIIGQESGGNKPANagSGSGAAGLFQFMPGTWKAYGKNS---SSDLNDPEASIEAA 56
LYZ_C_invert cd16899
C-type invertebrate lysozyme; C-type lysozyme proteins of invertebrates, including digestive ...
 58-129 2.38e-04

C-type invertebrate lysozyme; C-type lysozyme proteins of invertebrates, including digestive lysozymes 1 and 2 from Musca domestica, which aid in the use of bacteria as a food source. These lysozymes have high expression in the gut and optimal lytic activity at a lower pH. Other lysozymes in this subfamily have immunological roles. e.g. Anopheles gambiae has eight lysozymes, most of which seem to have immunological roles, those some may function as digestive enzymes in larvae. C-type lysozyme (chicken or conventional type; 1, 4-beta-N-acetylmuramidase) has bacteriolytic properties through the hydolysis of beta-1,4, glyocosidic linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan, as well as between N-acetyl-D-glucosamine residues in chitodextrins.


Pssm-ID: 381618 [Multi-domain]  Cd Length: 121  Bit Score: 40.29  E-value: 2.38e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1723593444  58 SDWDRLAQCESGGN-----WHINTGNGYYGGLQFSQGTWAAHGGTNY----APSADLATRDQQIAV--AEHVLATQGWGA 126
Cdd:cd16899    23 ANWVCLAEHESSFNttavgGPNSDGSGDYGLFQINDKYWCSPGGGSGngcnVKCEDLLDDDISDDVkcAKKIYKEQGFNA 102


                  ...
gi 1723593444 127 WPA 129
Cdd:cd16899   103 WVG 105
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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