|
Name |
Accession |
Description |
Interval |
E-value |
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
1-418 |
0e+00 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 527.41 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 1 MYALIDVNSMYCSCEEAFRPDLRGRPVVVLSNNDGALVAVNRAAKALGVRRGEPYFRCRRLLEQHNVAVFSSNYTLYASF 80
Cdd:PRK03609 1 MFALCDVNSFYASCETVFRPDLRGKPVVVLSNNDGCVIARSAEAKALGIKMGDPWFKQKDLFRRCGVVCFSSNYELYADM 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 81 SARFATVVESLAPRTFVYSIDELFADASHMEGVMTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKTW-PAT 159
Cdd:PRK03609 81 SNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWqRQT 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 160 GGVVALSDPVRLKKLMSLVAAGDVWGVGHRTEKGLAAMGIKTALALAEMDIRLARRLYGVTLERTIRELRGEACFALEEG 239
Cdd:PRK03609 161 GGVVDLSNLERQRKLLSLQPVEEVWGVGRRISKKLNAMGIKTALDLADTNIRFIRKHFNVVLERTVRELRGEPCLSLEEF 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 240 TGAKQQLVVSRSFGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAYDAGGVPYSNQATEVLPVPSQDTR 319
Cdd:PRK03609 241 APTKQEIVCSRSFGERITDYESMRQAICSYAARAAEKLRGEHQYCRFISTFVKTSPFALNEPYYGNSASVKLLTPTQDSR 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 320 DIIAAAQLGLSRIWREGVRYAKAGVMLSDL--QGYeTQLDLFSPAAVRPGSEKLMATIDKIN-REGRSRIFFAGEGIDPE 396
Cdd:PRK03609 321 DIIAAATRALDAIWRDGHRYQKAGVMLGDFfsQGV-AQLNLFDDNAPRPGSEQLMKVLDTLNaKSGKGTLYFAGQGIAQQ 399
|
410 420
....*....|....*....|..
gi 585302557 397 YAMRREMLSPAYTTQWKDIPAA 418
Cdd:PRK03609 400 WQMKREMLSPRYTTRWSDLLRV 421
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
3-348 |
2.61e-163 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 462.79 E-value: 2.61e-163
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 3 ALIDVNSMYCSCEEAFRPDLRGRPVVVLSNNDGALVAVNRAAKALGVRRGEPYFRCRRLLEQHNVAVFSSNYTLYASFSA 82
Cdd:cd01700 1 ALVDCNSFYASCERVFRPLLLGRPLVVLSNNDGCVIARSPEAKALGIKMGSPYFKVPDLLERHGVAVFSSNYALYGDMSR 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 83 RFATVVESLAPRTFVYSIDELFADASHMEGVMTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKTWPATGGV 162
Cdd:cd01700 81 RIMSILERFSPDVEVYSIDESFLDLTGSLRFGDLEELARKIRRRILQETGIPVTVGIGPTKTLAKLANDLAKKKNPYGGV 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 163 VALSDPVRLKKLMSLVAAGDVWGVGHRTEKGLAAMGIKTALALAEMDIRLARRLYGVTLERTIRELRGEACFALEEGTGA 242
Cdd:cd01700 161 VDLTDEEVRDKLLKILPVGDVWGIGRRTAKKLNAMGIHTAGDLAQADPDLLRKKFGVVGERLVRELNGIDCLPLEEYPPP 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 243 KQQLVVSRSFGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAYDAGgvPYSNQATEVLPVPSQDTRDII 322
Cdd:cd01700 241 KKSIGSSRSFGRDVTDLDELKQALAEYAERAAEKLRRQKSVARTISVFIGTSGFSRQ--PKYYSATNTLPYPTNDTREIV 318
|
330 340
....*....|....*....|....*.
gi 585302557 323 AAAQLGLSRIWREGVRYAKAGVMLSD 348
Cdd:cd01700 319 KAALRLLYAIYRPGYAYRKAGVMLSD 344
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
1-347 |
2.23e-106 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 317.47 E-value: 2.23e-106
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 1 MYALIDVNSMYCSCEEAFRPDLRGRPVVVLS-NNDGALVAVNRAAKALGVRRGEPYFRCRRLLEQhnVAVFSSNYTLYAS 79
Cdd:COG0389 2 RILHVDMDAFYASVEQRDRPELRGKPVAVGGdNNRGVVAAASYEARAFGVRSGMPLFQARRLCPD--LVVLPPDFELYRD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 80 FSARFATVVESLAPRTFVYSIDELFADASHMEGVM-TPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKTwpa 158
Cdd:COG0389 80 VSRRVMAILERYTPLVEPLSIDEAFLDVTGSARLFgSAEAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKP--- 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 159 tGGVVALsDPVRLKKLMSLVAAGDVWGVGHRTEKGLAAMGIKTALALAEMDIRLARRLYGVTLERTIRELRGEACFALEE 238
Cdd:COG0389 157 -DGLTVI-PPGEVAAFLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALPRAELRRRFGKVGERLYRLARGIDPRPVEP 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 239 gTGAKQQLVVSRSFGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAYDaggvpySNQATEVLPVPSQDT 318
Cdd:COG0389 235 -RRPRKSIGVERTFGEDLTDLEELEAALRRLAERLAERLRRQGLGARTVTVKLRTSDFR------TTTRSRTLPEPTDDT 307
|
330 340
....*....|....*....|....*....
gi 585302557 319 RDIIAAAQLGLSRIWREGVRYAKAGVMLS 347
Cdd:COG0389 308 AELLRAARELLERIYRPGRPVRLLGVRLS 336
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
5-349 |
6.46e-52 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 176.94 E-value: 6.46e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 5 IDVNSMYCSCEEAFRPDLRGRPVVVLSNND-GALVAVNRAAKALGVRRGEPYFRCRRLLEQhnvAVF-SSNYTLYASFSA 82
Cdd:cd03586 3 IDMDAFYASVEQRDNPELKGKPVAVGGSSDrGVVSTASYEARKFGVRSAMPIFQAKKLCPN---LIFvPPRFDKYREVSR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 83 RFATVVESLAPRTFVYSIDELFADASHMEGV-MTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKtwPAtGG 161
Cdd:cd03586 80 QIMEILREYTPLVEPLSIDEAYLDVTDYVRLfGSATEIAKEIRARIREETGLTASAGIAPNKFLAKIASDLNK--PN-GL 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 162 VValsdpVRLKKLMSLVAA---GDVWGVGHRTEKGLAAMGIKTALALAEMDIRLARRLYGVTLERTIRELRGEACFALEE 238
Cdd:cd03586 157 TV-----IPPEDVEEFLAPlpvRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNRPVEP 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 239 GTGAKqQLVVSRSFGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAYDAggvpYSNQATevLPVPSQDT 318
Cdd:cd03586 232 DRERK-SIGVERTFSEDLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKLKYADFST----RTRSRT--LPEPTDDA 304
|
330 340 350
....*....|....*....|....*....|..
gi 585302557 319 RDIIAAAQLGLSRIWRE-GVRyaKAGVMLSDL 349
Cdd:cd03586 305 EDIYELALELLEELLDGrPIR--LLGVRLSGL 334
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
5-347 |
7.84e-43 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 153.29 E-value: 7.84e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 5 IDVNSMYCSCEEAFRPDLRGRPVVVLSNND--GALVAVNRAAKALGVRRGEPYFRCRRLLEqhNVAVFSSNYTLYASFSA 82
Cdd:cd00424 3 IDFDNFFASVEQLARPELKGRPVVVVPFNSdsTCVIACSYEARKYGVKRGMPVREARKMCP--NLILVPARLDLYRRLSE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 83 RFATVVESLAPRTFVYSIDELFADASHMEGVMT-PQAFGQLLRAEVLRQT-TLTCGVGVAPTKTLAKLCNHAAKTWPATG 160
Cdd:cd00424 81 RLLSELEEVAPLVEVASIDELFLDLTGSARLLGlGSEVALRIKRHIAEQLgGITASIGIASNKLLAKLAAKYAKPDGLTI 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 161 GvvalsDPVRLKKLMSLVAAGDVWGVGHRTEKGLAAMGIKT--ALALAEMDIRLARRlYGVTLERTIRELRGEACFALEE 238
Cdd:cd00424 161 L-----DPEDLPGFLSKLPLTDLPGIGAVTAKRLEAVGINPigDLLAASPDALLALW-GGVSGERLWYALRGIDDEPLSP 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 239 GTGaKQQLVVSRSFGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRssaYDAGGVP-----YSNQAtevlPV 313
Cdd:cd00424 235 PRP-RKSFSHERVLPRDSRNAEDARPLLRLLLEKLARRLRRDGRGATRLRLWLR---TVDGRWSghadiPSRSA----PR 306
|
330 340 350
....*....|....*....|....*....|....*..
gi 585302557 314 PSQDTRDIIAAAQLGLSR---IWREGVRYAKAGVMLS 347
Cdd:cd00424 307 PISTEDGELLHALDKLWRallDDKGPRRLRRLGVRLS 343
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
5-148 |
4.85e-36 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 129.23 E-value: 4.85e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 5 IDVNSMYCSCEEAFRPDLRGRPVVVLSNNDGALV-AVNRAAKALGVRRGEPYFRCRRLLeqHNVAVFSSNYTLYASFSAR 83
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNGRGIVaAASYEARKYGVRSGMPVFEAKKLC--PNLIVVPPDLELYRRASRK 78
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 585302557 84 FATVVESLAPRT-FVYSIDELFADASHMEGVM-TPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKL 148
Cdd:pfam00817 79 IFEILRRFSTPKvEQASIDEAFLDLTGLEKLFgAEEALAKRLRREIAEETGLTCSIGIAPNKLLAKL 145
|
|
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
4-389 |
1.09e-34 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 132.82 E-value: 1.09e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 4 LIDVNSMYCSCEEAFRPDLRGRPVVVL---SNNDGALVAVNRAAKALGVRRGEPYFRCRRLLEQhnVAVFSSNYTLYASF 80
Cdd:PRK03103 7 LVDMQSFYASVEKAANPELKGRPVIVSgdpERRSGVVLAACPLAKAYGVKTAERLWEAQQKCPD--LVVVKPRMQRYIDV 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 81 SARFATVVESLAPRTFVYSIDELFADASHMEGVM-TPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKL-CNHAAKTWPA 158
Cdd:PRK03103 85 SLQITRILEDFTDLVEPFSIDEQFLDVTGSQKLFgSPLEIAQKIQQRIMRETGVYARVGIGPNKLLAKMaCDNFAKKNPD 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 159 tgGVVALSDPvRLKKLMSLVAAGDVWGVGHRTEKGLAAMGIKTALALAEMDIRLARRLYGVTLERTIRELRGE-----AC 233
Cdd:PRK03103 165 --GLFTLDKE-DVPADLWPLPVRKLFGVGSRMEKHLRRMGIRTIGQLANTPLERLKKRWGINGEVLWRTANGIdyspvTP 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 234 FALEEGTGAKQQLVVSRSFgarvTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAYDAgGVPYSNQATevLPV 313
Cdd:PRK03103 242 HSLDRQKAIGHQMTLPRDY----RGFEEIKVVLLELCEEVCRRARAKGYMGRTVSVSLRGADFDW-PTGFSRQMT--LPE 314
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 585302557 314 PSQDTRDIIAAAQLGLSRIWREG-VRyaKAGVMLSDLQGYET-QLDLFSPAAVRpgsEKLMATIDKI-NREGRSRIFFA 389
Cdd:PRK03103 315 PTNLAMEVYEAACKLFHRHWDGKpVR--RVGVTLSNLVSDDVwQLSLFGDRERK---RSLGYVMDDIkNRFGPTAILRA 388
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
5-359 |
2.17e-30 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 121.58 E-value: 2.17e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 5 IDVNSMYCSCEEAFRPDLRGRPVVVLSNNDGALVA-VNRAAKALGVRRGEPYFRCRRLLeQHNVAVFSSNYTLYASFSAR 83
Cdd:PRK03348 10 LDMDAFFASVEQLTRPTLRGRPVLVGGLGGRGVVAgASYEARVFGARSAMPMHQARRLV-GNGAVVLPPRFVVYRAASRR 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 84 FATVVESLAPRTFVYSIDELFADASHMEGVMTP--QAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKtwPATGG 161
Cdd:PRK03348 89 VFDTLRELSPVVEQLSFDEAFVEPAELAGASAEevEAFAERLRARVREETGLPASVGAGSGKQIAKIASGLAK--PDGIR 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 162 VVAlsdPVRLKKLMSLVAAGDVWGVGHRTEKGLAAMGIKTALALAEMDIRLARRLYGVTLERTIREL-RGEACFALEEGT 240
Cdd:PRK03348 167 VVP---PGEERELLAPLPVRRLWGIGPVTEEKLHRLGIETIGDLAALSEAEVANLLGATVGPALHRLaRGIDDRPVAERA 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 241 GAKqQLVVSRSFGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAYDAggvpYSNQATevLPVPSQDTRD 320
Cdd:PRK03348 244 EAK-QISAESTFAVDLTTRAQLREAIERIAEHAHRRLLKDGRGARTVTVKLRKSDFST----LTRSAT--LPYATDDAAV 316
|
330 340 350 360
....*....|....*....|....*....|....*....|
gi 585302557 321 IIAAAQ-LGLSRIWREGVRYakAGVMLSDLQGYeTQLDLF 359
Cdd:PRK03348 317 LAATARrLLLDPDEIGPIRL--VGVGFSGLSDV-RQESLF 353
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
5-379 |
1.68e-24 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 104.34 E-value: 1.68e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 5 IDVNSMYCSCEEAFRPDLRGRPVVVLSN---NDGALVAVNRAAKALGVRRGEPYFRCRRLLEQhnVAVFSSNYTLYASFS 81
Cdd:PRK01810 10 VDMNSFFASVEIAYDPSLQGKPLAVAGNekeRKGIIVTCSYEARAYGIRTTMPLWEAKRLCPQ--LIVRRPNFDRYREAS 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 82 ARFATVVESLAPRTFVYSIDELFADASHMEGVMTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLcnhaAKTWPATGG 161
Cdd:PRK01810 88 RQMFQILSEFTPLVQPVSIDEGYLDITDCYALGSPLEIAKMIQQRLLTELQLPCSIGIAPNKFLAKM----ASDMKKPLG 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 162 VVALSDPVRLKKLMSLvAAGDVWGVGHRTEKGLAAMGIKTALALAEMDIRLARRLYGVTLERTIRELRGEACFALE-EGT 240
Cdd:PRK01810 164 ITVLRKRDVPEMLWPL-PVGEMHGIGEKTAEKLKDIGIQTIGDLAKADEHILRAKLGINGVRLQRRANGIDDRPVDpEAI 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 241 GAKQQLVVSRSFGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRssaydaggvpYSNQAT----EVLPVPSQ 316
Cdd:PRK01810 243 YQFKSVGNSTTLSHDMDEEKELLDVLRRLSKSVSKRLQKKTVVSYNVQIMIR----------YHDRRTitrsKTLKNPIW 312
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 585302557 317 DTRDIIAAAQLGLSRIWREG-VRYakAGVMLSDL----QGYEtQLDLFSPAAvRPGSEKLMATIDKIN 379
Cdd:PRK01810 313 EKRDIFQAASRLFKQHWNGDpVRL--LGVTATDLewktEAVK-QLDLFSFEE-DAKEEPLLAVIDQIN 376
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
5-332 |
7.34e-22 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 95.86 E-value: 7.34e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 5 IDVNSMYCSCEEAFRPDLRGRPVVVLSNND-----GALVAVNRAAKALGVRRGEPyFR--CRRLLEqhnvAVF-SSNYTL 76
Cdd:PRK03352 10 VDLDQFIAAVELLRRPELAGLPVIVGGNGDpteprKVVTCASYEARAFGVRAGMP-LRtaARRCPD----AVFlPSDPAA 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 77 YASFSARFATVVESLAPRTFVYSIDELFADAshmeGVMTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKtw 156
Cdd:PRK03352 85 YDAASEEVMATLRDLGVPVEVWGWDEAFLGV----DTDDPEALAEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAK-- 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 157 PAtgGVVALSDPVRLKkLMSLVAAGDVWGVGHRTEKGLAAMGIKTALALAEMDIRLARRLYGVTLERTIREL-RGEAcfa 235
Cdd:PRK03352 159 PA--GVFRLTDANWMA-VMGDRPTDALWGVGPKTAKRLAALGITTVADLAAADPAELAATFGPTTGPWLLLLaRGGG--- 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 236 lEEGTGAKQQLVVSRS----FGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAYdaggvpYSNQATEVL 311
Cdd:PRK03352 233 -DTEVSAEPWVPRSRSrevtFPQDLTDRAEVESAVRELARRVLDEVVAEGRPVTRVAVKVRTATF------YTRTKIRKL 305
|
330 340
....*....|....*....|.
gi 585302557 312 PVPSQDTRDIIAAAQLGLSRI 332
Cdd:PRK03352 306 PEPTTDPDVIEAAALDVLDRF 326
|
|
| IMS_C |
pfam11799 |
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss). |
243-350 |
2.78e-20 |
|
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
Pssm-ID: 463354 [Multi-domain] Cd Length: 104 Bit Score: 85.30 E-value: 2.78e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 243 KQQLVVSRSFGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAYDAggvpYSNQATevLPVPSQDTRDII 322
Cdd:pfam11799 1 RKSIGAERTFGRDLTDLEELREALLELAEELAERLRRQGLVARTVTVKIRYSDFRT----ITRSVT--LPSPTDDTDEIY 74
|
90 100
....*....|....*....|....*...
gi 585302557 323 AAAQLGLSRIWReGVRYAKAGVMLSDLQ 350
Cdd:pfam11799 75 RAALRLLRRLYR-GRPVRLLGVSLSNLV 101
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
6-378 |
4.23e-20 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 91.20 E-value: 4.23e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 6 DVNSMYCSCEEAFRPDLRGRPVVVlsnNDGALVAVNRAAKALGVRRGEPYFRCRRLLEQhnvAVF-SSNYTLYASFSARF 84
Cdd:PRK03858 10 DLDSFYASVEQRDDPALRGRPVIV---GGGVVLAASYEAKAYGVRTAMGGRQARRLCPQ---AVVvPPRMSAYSRASKAV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 85 ATVVESLAPRTFVYSIDELFADAS---HMEGvmTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKtwpaTGG 161
Cdd:PRK03858 84 FEVFRDTTPLVEGLSIDEAFLDVGglrRISG--TPVQIAARLRRRVREEVGLPITVGVARTKFLAKVASQVAK----PDG 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 162 VVaLSDPVRLKKLMSLVAAGDVWGVGHRTEKGLAAMGIKTALALAEMDirlarrlyGVTLERTIRELRGEACFALEEG-- 239
Cdd:PRK03858 158 LL-VVPPDRELAFLHPLPVRRLWGVGPVTAAKLRAHGITTVGDVAELP--------ESALVSLLGPAAGRHLHALAHNrd 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 240 -----TGAKQQLVVS-RSFGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAYDAggvpysnqATE--VL 311
Cdd:PRK03858 229 prrveTGRRRRSVGAqRALGRGPNSPAEVDAVVVALVDRVARRMRAAGRTGRTVVLRLRFDDFTR--------ATRshTL 300
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 585302557 312 PVPSQDTRDIIAAAQL----GLSRIWREGVRYakAGVMLSDL-QGYETQLDLfsPAAVRPGSEKLMATIDKI 378
Cdd:PRK03858 301 PRPTASTATLLAAARDlvaaAAPLIAERGLTL--VGFAVSNLdDDGAQQLEL--PFGLRRPGSALDAALDAV 368
|
|
| DUF4113 |
pfam13438 |
Domain of unknown function (DUF4113); Although the function is not known this domain occurs ... |
371-416 |
9.64e-19 |
|
Domain of unknown function (DUF4113); Although the function is not known this domain occurs almost invariably at the very C-terminus of the IMS family DNA-polymerase repair proteins, IMS, pfam00817.
Pssm-ID: 463876 [Multi-domain] Cd Length: 49 Bit Score: 79.06 E-value: 9.64e-19
10 20 30 40
....*....|....*....|....*....|....*....|....*..
gi 585302557 371 LMATIDKIN-REGRSRIFFAGEGIDPEYAMRREMLSPAYTTQWKDIP 416
Cdd:pfam13438 1 LMAALDAINrRYGRGTVRLAAQGLKRDWKMRREHLSPRYTTRWDELP 47
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
1-199 |
8.49e-17 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 80.99 E-value: 8.49e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 1 MYALIDVNSMYCSCEEAFRPDLRGRPVVVL-----SNNDGALVAVNRAAKALGVRRGEPYFRCRRLLEQhnvAVF-SSNY 74
Cdd:PRK01216 2 IILFVDFDYFFAQVEEVLNPSLKGKPVVVCvysgrFEDSGAVATANYEARKLGIKAGMPIVEAKKILPN---AVYlPMRK 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 75 TLYASFSARFATVVESLAPRTFVYSIDELFADASH-MEGVMTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAA 153
Cdd:PRK01216 79 EVYQQVSNRIMKLLREYSEKIEIASIDEAYLDISDkVKNYQDAYNLGLEIKNKILEKEKITVTVGISKNKVFAKIAADMA 158
|
170 180 190 200
....*....|....*....|....*....|....*....|....*.
gi 585302557 154 KtwPATGGVValsDPVRLKKLMSLVAAGDVWGVGHRTEKGLAAMGI 199
Cdd:PRK01216 159 K--PNGIKVI---DDEEVKRFINELDIADIPGIGDITAEKLKKLGV 199
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
2-296 |
6.81e-13 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 70.43 E-value: 6.81e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 2 YALIDVNSMYCSCEEAFRPDLRGRPVVVLSNNdgALVAVNRAAKALGVRRGEPYFRCRRLLEqhNVAVFSSNYTLYASFS 81
Cdd:PTZ00205 135 YIHLDMDMFYAAVEIKKHPEYAAIPLAIGTMT--MLQTANYVARGRGIRQGMPGFLALKICP--NLLILPPDFDAYNEES 210
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 82 ARFATVVESLAPRTFVYSIDELFADAS----HMEGVMTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKtwp 157
Cdd:PTZ00205 211 NTVRRIVAEYDPNYISFGLDELTLEVSayieRFEGTKTAEDVASELRVRVFGETKLTASAGIGPTAALAKIASNINK--- 287
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 158 aTGGVVALSDPVRlKKLMSLV---AAGDVWGVGHRTEKGLAAMGIKTalaLAEMDIRLARRLYGVTlERTIRELRG---- 230
Cdd:PTZ00205 288 -PNGQHDLNLHTR-GDVMTYVrdlGLRSVPGVGKVTEALLKGLGITT---LSDIYNRRVELCYILH-NNLFRFLLGasig 361
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 585302557 231 -----EACFAL-----EEGTGAKQQLVVS-RSFGARVTQlAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAY 296
Cdd:PTZ00205 362 imqwpDAATAAntencEGATGGQRKAISSeRSFTTPRTK-EGLQEMVDTVFNGAYEEMRKSELMCRQISLTIRWASY 437
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
11-359 |
1.18e-12 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 68.61 E-value: 1.18e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 11 YCSCEEAFRPDLRGRPVVVLSNND--GALVAVNRAAKALGVRRGEPYFRCRRLLEQhnvAVF-SSNYTLYASFS------ 81
Cdd:PRK02406 5 YAAVEMRDNPELRGKPVAVGGSPGrrGVISTCNYEARKFGVRSAMPTAQALKLCPD---LIFvPGRFDVYKEVSrqirei 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 82 -ARFATVVESLaprtfvySIDELFADASHmegvmTPQAFG------QLLRAEVLRQTTLTCGVGVAPTKTLAKlcnhaak 154
Cdd:PRK02406 82 fRRYTDLIEPL-------SLDEAYLDVTD-----NKLCIGsatliaQEIRQDIFEELGLTASAGVAPNKFLAK------- 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 155 twpatggvVAlSD---P-----VRLKKLMSLVAA---GDVWGVGHRTEKGLAAMGIKTALALAEMDI--------RLARR 215
Cdd:PRK02406 143 --------IA-SDwnkPnglfvITPEEVDAFLATlpvEKIPGVGKVTAEKLHALGIYTCADLQKYDLaelirhfgKFGRR 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 216 LYGvtLERTI--RELRGEAcfaleegtgAKQQLVVSRSFGARVTQLAQMQQAVTKYATRAGEKLR--QENRLAKVVTVFI 291
Cdd:PRK02406 214 LYE--RARGIdeRPVKPDR---------ERKSVGVERTFAEDLYDLEACLAELPRLAEKLERRLEraKPDKRIKTVGVKL 282
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 585302557 292 RSSAYdaggvpysNQAT-EVLPVPSQDTRDIIAAAQLGLSRIWREGVRYAKAGVMLSDLQGyETQLDLF 359
Cdd:PRK02406 283 KFADF--------QQTTkEHTADPLDKADLIELLAQALLRRLGGRGVRLLGVGVTLLEPQL-ERQLLLD 342
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
5-291 |
1.30e-12 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 68.88 E-value: 1.30e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 5 IDVNSMYCSCEEAFRPDLRGRPVVVLSN-NDGALVA-VNRAAKALGVRRGEPYFRCRRLLEQhnVAVFSSNYTLYASFSA 82
Cdd:cd01701 52 VDFDCFFVSVSIRNRPDLKGKPVAVCHGkGPNSEIAsCNYEARSYGIKNGMWVGQAKKLCPQ--LVTLPYDFEAYEEVSL 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 83 RFATVVESLAPRTFVYSIDELFADASHM--EGVMTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKtwPAtg 160
Cdd:cd01701 130 TFYEILASYTDNIEAVSCDEALIDITSLleETYELPEELAEAIRNEIRETTGCSASVGIGPNILLARLATRKAK--PD-- 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 161 GVVALSdPVRLKKLMSLVAAGDVWGVGHRT-EKGLAAMG---IKTALALAEMDiRLARRLYGVTLERTIRELRGEACfal 236
Cdd:cd01701 206 GQYHLS-AEKVEEFLSQLKVGDLPGVGSSLaEKLVKLFGdtcGGLELRSKTKE-KLQKVLGPKTGEKLYDYCRGIDD--- 280
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*..
gi 585302557 237 EEGTGAKQQLVVSRS--FGARVTQLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFI 291
Cdd:cd01701 281 RPVTGEKERKSVSAEinYGIRFTNVDDVEQFLQRLSEELSKRLEESNVTGRQITLKL 337
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
3-231 |
8.17e-12 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 66.49 E-value: 8.17e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 3 ALIDVNSMYCSCEEAFRPDLRGRPVVVLSNNDGALVAVNRAAKALGVRRGEPYFRCRRLLEQhnVAVFSSNYTLYASFSA 82
Cdd:PRK02794 39 AHIDCDAFYASVEKRDNPELRDKPVIIGGGKRGVVSTACYIARIHGVRSAMPMFKALKLCPD--AVVIKPDMEKYVRVGR 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 83 RFATVVESLAPRTFVYSIDELFADASHMEGV--MTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKT--WPA 158
Cdd:PRK02794 117 EVRAMMQALTPLVEPLSIDEAFLDLSGTERLhgAPPAVVLARFARRVEREIGITVSVGLSYNKFLAKIASDLDKPrgFSV 196
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 585302557 159 TGGVVALS----DPVRLkklmslvaagdVWGVGHRTEKGLAAMGIKTALALAEMDIRLARRLYGVTLERTIRELRGE 231
Cdd:PRK02794 197 IGRAEALAflapKPVGI-----------IWGVGPATAARLARDGIRTIGDLQRADEADLMRRFGSMGLRLWRLARGI 262
|
|
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
3-336 |
2.20e-11 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 64.64 E-value: 2.20e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 3 ALIDVNSMYCSCEEAFRPDLRGRPVVVLSNNdgALVAVNRAAKALGVRRG----EPYFRCRRLLEQHnVAVFSS------ 72
Cdd:cd01702 1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWN--SIIAVSYAARAFGVTRFmtidEAKKKCPDLILAH-VATYKKgedead 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 73 ------------NYTLYASFSARFATVVESLAPRTFVYSIDELFAD--ASHMEGVmtpqafgqllRAEVLRQTTLTCGVG 138
Cdd:cd01702 78 yhenpsparhkvSLDPYRRASRKILNILKRFGDVVEKASIDEAYLDlgSRIVEEI----------RQQVYDELGYTCSAG 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 139 VAPTKTLAKLCNH----AAKTWPATGGVVALSDPVRLKKLMSLvaaGDVWGVGHRTEKGLAAMGIKTALALAEMDIrlaR 214
Cdd:cd01702 148 IAHNKMLAKLASGmnkpNAQTILRNDAVASFLSSLPITSIRGL---GGKLGEEIIDLLGLPTEGDVAGFRSSESDL---Q 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 215 RLYGVTLERTI-RELRGEACFALEEgTGAKQQLVVSRSF-GARVTQLAQMQQAVTKYAT----RAGEKLRQENRLAKVVT 288
Cdd:cd01702 222 EHFGEKLGEWLyNLLRGIDHEPVKP-RPLPKSMGSSKNFpGKTALSTEDVQHWLLVLASelnsRLEDDRYENNRRPKTLV 300
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 585302557 289 VFIRSsayDAGGVPYSNQAtevlPVPSQDTRDIIAAAQLGLSRIWREG 336
Cdd:cd01702 301 LSLRQ---RGDGVRRSRSC----ALPRYDAQKIVKDAFKLIKAINEEG 341
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
5-298 |
5.22e-11 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 63.58 E-value: 5.22e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 5 IDVNSMYCSCEEAFRPDLRGRPVVV--LSNNdGALVAVNRAAKALGVRRGEPYFRCRRLLEqHNVAVfSSNYTLYASFSA 82
Cdd:PRK14133 8 VDMDAFFASVEQMDNPKLKGKPVIVggISER-GVVSTCSYEARKYGVHSAMPVFMAKKRCP-HGIFL-PVRHERYKEVSK 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 83 RFATVVESLAPRTFVYSIDELFADASHMEgvMTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLcnhaAKTWPATGGV 162
Cdd:PRK14133 85 NIFKILYEVTPIVEPVSIDEAYLDITNIK--EEPIKIAKYIKKKVKKETGLTLSVGISYNKFLAKL----ASDWNKPDGI 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 163 VALSDPVRLKKLMSLvAAGDVWGVGHRTEKGLAAMGIKTALALAEMDIRLARRL---YGVTLERTIRELRGeacfaleeg 239
Cdd:PRK14133 159 KIITEDMIPDILKPL-PISKVHGIGKKSVEKLNNIGIYTIEDLLKLSREFLIEYfgkFGVEIYERIRGIDY--------- 228
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 585302557 240 tgakQQLVVSR---SFGARVT------QLAQMQQAVTKYATRAGEKLRQENRLAKVVTVFIRSSAYDA 298
Cdd:PRK14133 229 ----REVEVSRerkSIGKETTlkkdtkDKEELKKYLKDFSNIISEELKKRNLYGKTVTVKIKTSDFQT 292
|
|
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
6-245 |
1.66e-08 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 55.94 E-value: 1.66e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 6 DVNSMYCSCEEAFRPDLRGRPVVVLSNNdgALVAVNRAAKALGVRR----GEPYFRCRRLleqhnVAVFSSNYTLYASFS 81
Cdd:cd01703 4 DLDCFYAQVEEIRDPSLKSKPLGIQQKY--IVVTCNYEARRLGVKKlmsiKDAKEICPDL-----VLVNGEDLTPFRDMS 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 82 ARFATVVESLAPRTFV--YSIDELFADASHMEGVMTPQaFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAK----- 154
Cdd:cd01703 77 KKVYRLLRSYSWNDRVerLGFDENFMDVTEMRLLVASH-IAYEMRERIENELGLTCCAGIASNKLLAKLVGSVNKpnqqt 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 155 --TWPATGGVVALSDPVRLKKLMslvaagdvwGVGHRTEKGLAAMGIKTALALAEMDIRLaRRLYGVTLERTIRELRGEa 232
Cdd:cd01703 156 tlLPPSCADLMDFMDLHDLRKIP---------GIGYKTAAKLEAHGISSVRDLQEFSNRN-RQTVGAAPSLLELLLMVK- 224
|
250
....*....|...
gi 585302557 233 cfALEEGTGAKQQ 245
Cdd:cd01703 225 --EFGEGIGQRIW 235
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
19-298 |
7.59e-07 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 50.84 E-value: 7.59e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 19 RPDLRGRPVVVLSNNDGA-LVAVNRAAKALGVRRGEPYFRCRRLleQHNVAVFSSNYTLYASFSARFATVVESLAPRTFV 97
Cdd:cd03468 17 RPADDEAPLAVVERKKAGrILACNAAARAAGVRPGMPLAEALAL--CPNLQVVEYDPEADARALQELALWLLRFTPLVAL 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 98 YSIDELFADAS---HMEGVMtpQAFGQLLRAEVLRQtTLTCGVGVAPTKTLAKLCNHAAKTWPATGGVVALSDPVRLKKL 174
Cdd:cd03468 95 DGPDGLLLDVTgclHLFGGE--DALAASLRAALATL-GLSARAGIADTPGAAWLLARAGGGRGVLRREALAAALVLLAPL 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585302557 175 msLVAAGdvwGVGHRTEKGLAAMGIKTALALAEMDIRLARRLYGVTLERTIRELRGEA-----CFA------------LE 237
Cdd:cd03468 172 --PVAAL---RLPPETVELLARLGLRTLGDLAALPRAELARRFGLALLLRLDQAYGRDpepllFSPpppafdfrlelqLE 246
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 585302557 238 EGTGAKQQLVVSRSFGARVTQLaQMQQAVtkyATRAGEKLRQENRLAKVVTVFIRSSAYDA 298
Cdd:cd03468 247 EPIARGLLFPLRRLLEQLCAFL-ALRGLG---ARRLSLTLFREDGRVTRVLVGLARPSRDD 303
|
|
| |
