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Conserved domains on  [gi|2217378149|ref|XP_047279668|]
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solute carrier family 28 member 3 isoform X3 [Homo sapiens]

Protein Classification

NupC/NupG family nucleoside CNT transporter( domain architecture ID 1750464)

NupC/NupG family nucleoside CNT transporter similar to Homo sapiens sodium/nucleoside cotransporter 1 isoform 3 that selectively transports pyrimidine deoxyribonucleosides

Gene Ontology:  GO:0005337|GO:1901642|GO:0005886
TCDB:  2.A.41

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
nupC super family cl40802
nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) ...
 229-458 4.85e-84

nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) Members of the CNT family mediate nucleoside uptake. In bacteria they are energized by H+ symport, but in mammals they are energized by Na+ symport. The different transporters exhibit differing specificities for nucleosides. The E. coli NupC permease transports all nucleosides (both ribo- and deoxyribonucleosides) except hypoxanthine and guanine nucleosides. The B. subtilis NupC is specific for pyrimidine nucleosides (cytidine and uridine and the corresponding deoxyribonucleosides). The mammalian permease members of the CNT family also exhibit differing specificities. Thus, rats possess at least two NupC homologues, one specific for both purine and pyrimidine nucleosides and one specific for purine nucleosides. At least three paralogues have been characterized from humans. One human homologue(CNT1) transports pyrimidine nucleosides and adenosine, but deoxyadenosine and guanosine are poor substrates of this permease. Another (CNT2) is selective for purine nucleosides. Alteration of just a few amino acyl residues in TMSs 7 and 8 interconverts their specificities. [Transport and binding proteins, Nucleosides, purines and pyrimidines]


The actual alignment was detected with superfamily member TIGR00804:

Pssm-ID: 273278 [Multi-domain]  Cd Length: 401  Bit Score: 265.32  E-value: 4.85e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 229 LVSFGGLIMYIVLLFLFSKYPTRVYWRPVLWGIGLQFLLGLLILRTDPGFIAFDWLGRQVQTFLEYTDAGASFVFGEKYK 308
Cdd:TIGR00804   1 LISFVGLVVFILIAFLCSSNKKAISWRTVVSALVLQFLLGLIVLRTPPGFWAFQGLADQIQILLSYANEGSSFVFGPPLV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 309 D--HFFAFKVLPIVVFFSTVMSMLYYLGLMQWIIRKVGWIMLVTTGSSPIESVVASGNIFVGQTESPLLVRPYLPYITKS 386
Cdd:TIGR00804  81 QdlFVFAFQVLPIIIFFSALISILYYLGIMQWVIRKIGWFLQKAMGTTKLESFSAAANIFLGQTEAPLVIRPYLGDMTDS 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2217378149 387 ELHAIMTAGFSTIAGSVLGAYISFGVPSSHLLTASVMSAPASLAAAKLFWPETEKPKITLKNAMKMESGSCN 458
Cdd:TIGR00804 161 ELFTVMTSGMATIAGSVLGAYISMGVPATYLIAASVMAAPCALAFAKLIYPEVEESKEKSEEDVKLEEGDQN 232
 
Name Accession Description Interval E-value
nupC TIGR00804
nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) ...
 229-458 4.85e-84

nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) Members of the CNT family mediate nucleoside uptake. In bacteria they are energized by H+ symport, but in mammals they are energized by Na+ symport. The different transporters exhibit differing specificities for nucleosides. The E. coli NupC permease transports all nucleosides (both ribo- and deoxyribonucleosides) except hypoxanthine and guanine nucleosides. The B. subtilis NupC is specific for pyrimidine nucleosides (cytidine and uridine and the corresponding deoxyribonucleosides). The mammalian permease members of the CNT family also exhibit differing specificities. Thus, rats possess at least two NupC homologues, one specific for both purine and pyrimidine nucleosides and one specific for purine nucleosides. At least three paralogues have been characterized from humans. One human homologue(CNT1) transports pyrimidine nucleosides and adenosine, but deoxyadenosine and guanosine are poor substrates of this permease. Another (CNT2) is selective for purine nucleosides. Alteration of just a few amino acyl residues in TMSs 7 and 8 interconverts their specificities. [Transport and binding proteins, Nucleosides, purines and pyrimidines]


Pssm-ID: 273278 [Multi-domain]  Cd Length: 401  Bit Score: 265.32  E-value: 4.85e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 229 LVSFGGLIMYIVLLFLFSKYPTRVYWRPVLWGIGLQFLLGLLILRTDPGFIAFDWLGRQVQTFLEYTDAGASFVFGEKYK 308
Cdd:TIGR00804   1 LISFVGLVVFILIAFLCSSNKKAISWRTVVSALVLQFLLGLIVLRTPPGFWAFQGLADQIQILLSYANEGSSFVFGPPLV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 309 D--HFFAFKVLPIVVFFSTVMSMLYYLGLMQWIIRKVGWIMLVTTGSSPIESVVASGNIFVGQTESPLLVRPYLPYITKS 386
Cdd:TIGR00804  81 QdlFVFAFQVLPIIIFFSALISILYYLGIMQWVIRKIGWFLQKAMGTTKLESFSAAANIFLGQTEAPLVIRPYLGDMTDS 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2217378149 387 ELHAIMTAGFSTIAGSVLGAYISFGVPSSHLLTASVMSAPASLAAAKLFWPETEKPKITLKNAMKMESGSCN 458
Cdd:TIGR00804 161 ELFTVMTSGMATIAGSVLGAYISMGVPATYLIAASVMAAPCALAFAKLIYPEVEESKEKSEEDVKLEEGDQN 232
NupC COG1972
Nucleoside permease NupC [Nucleotide transport and metabolism];
227-454 8.97e-78

Nucleoside permease NupC [Nucleotide transport and metabolism];


Pssm-ID: 441575 [Multi-domain]  Cd Length: 406  Bit Score: 248.85  E-value: 8.97e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 227 QQLVSFGGLIMYIVLLFLFSKYPTRVYWRPVLWGIGLQFLLGLLILRTDPGFIAFDWLGRQVQTFLEYTDAGASFVFG-- 304
Cdd:COG1972     2 NILIGLLGIAVLLGIAWLFSENRKAINWRTVGIGLALQLLLAFLVLKVPAGRAAFEALSDGVNKLLSYANEGTAFVFGgl 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 305 ---EKYKDHFFAFKVLPIVVFFSTVMSMLYYLGLMQWIIRKVGWIMLVTTGSSPIESVVASGNIFVGQTESPLLVRPYLP 381
Cdd:COG1972    82 agpGLPGGFVFAFQVLPPIIFFSALISILYYLGILQFIVRGIGWVLSKILGTSGAESLSAAANIFLGQTEAPLVIKPYLA 161

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2217378149 382 YITKSELHAIMTAGFSTIAGSVLGAYISF-GVPSSHLLTASVMSAPASLAAAKLFWPETEKPKITLKNAMKMES 454
Cdd:COG1972   162 KMTRSELFTVMTGGMATVAGSVLAAYASMlGVPAKYLLAASVMAAPAALLIAKILVPETEEPETDGVVEVEEEK 235
Gate pfam07670
Nucleoside recognition; This region in the nucleoside transporter proteins are responsible for ...
 313-411 3.60e-17

Nucleoside recognition; This region in the nucleoside transporter proteins are responsible for determining nucleoside specificity in the human CNT1 and CNT2 proteins. In the FeoB proteins, which are believed to be Fe2+ transporters, it includes the membrane pore region, so the function of this region is likely to be more general than just nucleoside specificity. This family may represent the pore and gate, with a wide potential range of specificity. Hence its name 'Gate'.


Pssm-ID: 429586 [Multi-domain]  Cd Length: 101  Bit Score: 76.91  E-value: 3.60e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 313 AFKVLPIVVFFSTVMSMLYYLGLMQWIIRKVGWIMLVTtgSSPIESVVASGNIFVGQTE----SPLLVRPYLPYITKSEL 388
Cdd:pfam07670   1 LLKVLPIILFFSVLISILEYSGLLDRIGKLLGPLMRPL--GLFPLPGKAAIALLLGFGAkevgVPLLATPYGIDTPRERL 78

                          90       100
                  ....*....|....*....|...
gi 2217378149 389 HAIMTAGFSTIAGSVLGAYISFG 411
Cdd:pfam07670  79 AALLFTSFSTPCGATLPVYAGEL 101
 
Name Accession Description Interval E-value
nupC TIGR00804
nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) ...
 229-458 4.85e-84

nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) Members of the CNT family mediate nucleoside uptake. In bacteria they are energized by H+ symport, but in mammals they are energized by Na+ symport. The different transporters exhibit differing specificities for nucleosides. The E. coli NupC permease transports all nucleosides (both ribo- and deoxyribonucleosides) except hypoxanthine and guanine nucleosides. The B. subtilis NupC is specific for pyrimidine nucleosides (cytidine and uridine and the corresponding deoxyribonucleosides). The mammalian permease members of the CNT family also exhibit differing specificities. Thus, rats possess at least two NupC homologues, one specific for both purine and pyrimidine nucleosides and one specific for purine nucleosides. At least three paralogues have been characterized from humans. One human homologue(CNT1) transports pyrimidine nucleosides and adenosine, but deoxyadenosine and guanosine are poor substrates of this permease. Another (CNT2) is selective for purine nucleosides. Alteration of just a few amino acyl residues in TMSs 7 and 8 interconverts their specificities. [Transport and binding proteins, Nucleosides, purines and pyrimidines]


Pssm-ID: 273278 [Multi-domain]  Cd Length: 401  Bit Score: 265.32  E-value: 4.85e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 229 LVSFGGLIMYIVLLFLFSKYPTRVYWRPVLWGIGLQFLLGLLILRTDPGFIAFDWLGRQVQTFLEYTDAGASFVFGEKYK 308
Cdd:TIGR00804   1 LISFVGLVVFILIAFLCSSNKKAISWRTVVSALVLQFLLGLIVLRTPPGFWAFQGLADQIQILLSYANEGSSFVFGPPLV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 309 D--HFFAFKVLPIVVFFSTVMSMLYYLGLMQWIIRKVGWIMLVTTGSSPIESVVASGNIFVGQTESPLLVRPYLPYITKS 386
Cdd:TIGR00804  81 QdlFVFAFQVLPIIIFFSALISILYYLGIMQWVIRKIGWFLQKAMGTTKLESFSAAANIFLGQTEAPLVIRPYLGDMTDS 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2217378149 387 ELHAIMTAGFSTIAGSVLGAYISFGVPSSHLLTASVMSAPASLAAAKLFWPETEKPKITLKNAMKMESGSCN 458
Cdd:TIGR00804 161 ELFTVMTSGMATIAGSVLGAYISMGVPATYLIAASVMAAPCALAFAKLIYPEVEESKEKSEEDVKLEEGDQN 232
NupC COG1972
Nucleoside permease NupC [Nucleotide transport and metabolism];
227-454 8.97e-78

Nucleoside permease NupC [Nucleotide transport and metabolism];


Pssm-ID: 441575 [Multi-domain]  Cd Length: 406  Bit Score: 248.85  E-value: 8.97e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 227 QQLVSFGGLIMYIVLLFLFSKYPTRVYWRPVLWGIGLQFLLGLLILRTDPGFIAFDWLGRQVQTFLEYTDAGASFVFG-- 304
Cdd:COG1972     2 NILIGLLGIAVLLGIAWLFSENRKAINWRTVGIGLALQLLLAFLVLKVPAGRAAFEALSDGVNKLLSYANEGTAFVFGgl 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 305 ---EKYKDHFFAFKVLPIVVFFSTVMSMLYYLGLMQWIIRKVGWIMLVTTGSSPIESVVASGNIFVGQTESPLLVRPYLP 381
Cdd:COG1972    82 agpGLPGGFVFAFQVLPPIIFFSALISILYYLGILQFIVRGIGWVLSKILGTSGAESLSAAANIFLGQTEAPLVIKPYLA 161

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2217378149 382 YITKSELHAIMTAGFSTIAGSVLGAYISF-GVPSSHLLTASVMSAPASLAAAKLFWPETEKPKITLKNAMKMES 454
Cdd:COG1972   162 KMTRSELFTVMTGGMATVAGSVLAAYASMlGVPAKYLLAASVMAAPAALLIAKILVPETEEPETDGVVEVEEEK 235
Gate pfam07670
Nucleoside recognition; This region in the nucleoside transporter proteins are responsible for ...
 313-411 3.60e-17

Nucleoside recognition; This region in the nucleoside transporter proteins are responsible for determining nucleoside specificity in the human CNT1 and CNT2 proteins. In the FeoB proteins, which are believed to be Fe2+ transporters, it includes the membrane pore region, so the function of this region is likely to be more general than just nucleoside specificity. This family may represent the pore and gate, with a wide potential range of specificity. Hence its name 'Gate'.


Pssm-ID: 429586 [Multi-domain]  Cd Length: 101  Bit Score: 76.91  E-value: 3.60e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217378149 313 AFKVLPIVVFFSTVMSMLYYLGLMQWIIRKVGWIMLVTtgSSPIESVVASGNIFVGQTE----SPLLVRPYLPYITKSEL 388
Cdd:pfam07670   1 LLKVLPIILFFSVLISILEYSGLLDRIGKLLGPLMRPL--GLFPLPGKAAIALLLGFGAkevgVPLLATPYGIDTPRERL 78

                          90       100
                  ....*....|....*....|...
gi 2217378149 389 HAIMTAGFSTIAGSVLGAYISFG 411
Cdd:pfam07670  79 AALLFTSFSTPCGATLPVYAGEL 101
Nucleos_tra2_N pfam01773
Na+ dependent nucleoside transporter N-terminus; This family consists of nucleoside transport ...
 234-305 1.26e-14

Na+ dependent nucleoside transporter N-terminus; This family consists of nucleoside transport proteins. Swiss:Q62773 is a purine-specific Na+-nucleoside cotransporter localized to the bile canalicular membrane. Swiss:Q62674 is a a Na+-dependent nucleoside transporter selective for pyrimidine nucleosides and adenosine it also transports the anti-viral nucleoside analogues AZT and ddC. This alignment covers the N terminus of this family


Pssm-ID: 460319 [Multi-domain]  Cd Length: 73  Bit Score: 68.55  E-value: 1.26e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2217378149 234 GLIMYIVLLFLFSKYPTRVYWRPVLWGIGLQFLLGLLILRTDPGFIAFDWLGRQVQTFLEYTDAGASFVFGE 305
Cdd:pfam01773   1 GLLVLLGIAWLFSKNRKAINWRTVIGGLALQFLLALFVLRTPAGRDAFKWISDGVTALLGYADAGTAFVFGG 72
Nucleos_tra2_C pfam07662
Na+ dependent nucleoside transporter C-terminus; This family consists of nucleoside transport ...
 416-454 1.04e-09

Na+ dependent nucleoside transporter C-terminus; This family consists of nucleoside transport proteins. Swiss:Q62773 is a purine-specific Na+-nucleoside cotransporter localized to the bile canalicular membrane. Swiss:Q62674 is a a Na+-dependent nucleoside transporter selective for pyrimidine nucleosides and adenosine it also transports the anti-viral nucleoside analogues AZT and ddC. This alignment covers the C-terminus of this family of transporters.


Pssm-ID: 462223  Cd Length: 205  Bit Score: 58.18  E-value: 1.04e-09
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 2217378149 416 HLLTASVMSAPASLAAAKLFWPETEKPKITLKNAMKMES 454
Cdd:pfam07662   1 HLLTASVMSAPAALAISKLLYPETEEPPVKTGDEEEPAN 39
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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