PWWP domain found in methyl-CpG-binding domain protein 5 (MBD5) and similar proteins; MBD5, is a methyl-CpG-binding protein that binds to heterochromatin. It does not interact with either methylated or unmethylated DNA. MBD5 acts as a transcriptional regulator responsible for 2q23.1 deletion syndrome. It belongs to the MBD family proteins, which play central roles in transcriptional regulation and development. The PWWP domain specifically recognizes DNA and histone methylated lysines.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462575169 1501 FNVGDLVWGQIKGLTSWPGKLVREDDVHNScqqspEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNRKSGK 1580
Cdd:cd20141      1 FNVGDLVWGQIRGFPSWPGKLVSENDVGKT-----NEGKVWVSWFGDHSFGQVEPDKLKTLSEGLEAHHRARKRTRKGRK 75

                           90
                   ....*....|....*..
gi 2462575169 1581 LNNHLEAAIHEAMSELD 1597
Cdd:cd20141     76 LNNHLEAAIQEAMSELD 92

PWWP domain found in methyl-CpG-binding domain protein 5 (MBD5) and similar proteins; MBD5, is a methyl-CpG-binding protein that binds to heterochromatin. It does not interact with either methylated or unmethylated DNA. MBD5 acts as a transcriptional regulator responsible for 2q23.1 deletion syndrome. It belongs to the MBD family proteins, which play central roles in transcriptional regulation and development. The PWWP domain specifically recognizes DNA and histone methylated lysines.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462575169 1501 FNVGDLVWGQIKGLTSWPGKLVREDDVHNScqqspEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNRKSGK 1580
Cdd:cd20141      1 FNVGDLVWGQIRGFPSWPGKLVSENDVGKT-----NEGKVWVSWFGDHSFGQVEPDKLKTLSEGLEAHHRARKRTRKGRK 75

                           90
                   ....*....|....*..
gi 2462575169 1581 LNNHLEAAIHEAMSELD 1597
Cdd:cd20141     76 LNNHLEAAIQEAMSELD 92

domain with conserved PWWP motif; conservation of Pro-Trp-Trp-Pro residues

                            10        20        30        40        50        60
                    ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2462575169  1501 FNVGDLVWGQIKGLTSWPGKLV-REDDVHNSCQQSPEEGKVWVMWFGLHTFTQVEPEKLKTLT 1562
Cdd:smart00293    1 FKPGDLVWAKMKGFPWWPALVIsPKMTPDNIMKRKSDENLYPVLFFGDKDTAWIPSSKLFPLT 63

PWWP domain found in methyl-CpG-binding domain protein 5 (MBD5) and similar proteins; MBD5, is a methyl-CpG-binding protein that binds to heterochromatin. It does not interact with either methylated or unmethylated DNA. MBD5 acts as a transcriptional regulator responsible for 2q23.1 deletion syndrome. It belongs to the MBD family proteins, which play central roles in transcriptional regulation and development. The PWWP domain specifically recognizes DNA and histone methylated lysines.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462575169 1501 FNVGDLVWGQIKGLTSWPGKLVREDDVHNScqqspEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNRKSGK 1580
Cdd:cd20141      1 FNVGDLVWGQIRGFPSWPGKLVSENDVGKT-----NEGKVWVSWFGDHSFGQVEPDKLKTLSEGLEAHHRARKRTRKGRK 75

                           90
                   ....*....|....*..
gi 2462575169 1581 LNNHLEAAIHEAMSELD 1597
Cdd:cd20141     76 LNNHLEAAIQEAMSELD 92

PWWP domain; The PWWP domain is named after a conserved Pro-Trp-Trp-Pro motif. The domain binds to Histone-4 methylated at lysine-20, H4K20me, suggesting that it is methyl-lysine recognition motif. Removal of two conserved aromatic residues in a hydrophobic cavity created by this domain within the full-length protein, Pdp1, abolishes the interaction o f the protein with H4K20me3. In fission yeast, Set9 is the sole enzyme that catalyzes all three states of H4K20me, and Set9-mediated H4K20me is required for efficient recruitment of checkpoint protein Crb2 to sites of DNA damage. The methylation of H4K20 is involved in a diverse array of cellular processes, such as organizing higher-order chromatin, maintaining genome stability, and regulating cell-cycle progression.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462575169 1504 GDLVWGQIKGLTSWPGKLVREDDVHNSC-QQSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNRKSGKln 1582
Cdd:pfam00855    1 GDLVWAKLKGYPWWPARVVDPEELPENVlKPKKKDGEYLVRFFGDSEFAWVKPKDLKPFDEGDEFEYLKKKKKKKKKK-- 78

                           90
                   ....*....|....*
gi 2462575169 1583 nHLEAAIHEAMSELD 1597
Cdd:pfam00855   79 -AFKKALEEAEEALK 92

PWWP domain found in DNA (cytosine-5)-methyltransferase 3A (DNMT3A); DNMT3A, also called DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, or M.HsaIIIA, is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. It modifies DNA in a non-processive manner and also methylates non-CpG sites. It may preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. DNMT3A is recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. It contains a PWWP domain that binds methylated histone.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462575169 1499 RTFNVGDLVWGQIKGLTSWPGKLVREDDVHNScqqSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAY 1568
Cdd:cd20154      4 RGFGIGELVWGKLRGFSWWPGRIVSWWMTGRS---RAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFSSAF 70

PWWP domain found in glyoxylate reductase 1 (GLYR1) and similar proteins; GLYR1, also called 3-hydroxyisobutyrate dehydrogenase-like protein, cytokine-like nuclear factor N-PAC, nuclear protein NP60, or nuclear protein of 60 kDa, is a putative oxidoreductase that is recruited on chromatin and promotes KDM1B demethylase activity. It recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3). GLYR1 enhances the activity of MAP2K4 and MAP2K6 kinases to phosphorylate p38-alpha. In addition to the PWWP domain, GLYR1 also contains an AT-hook and a C-terminal NAD-binding domain. The PWWP domain specifically recognizes DNA and histone methylated lysines.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2462575169 1501 FNVGDLVWGQIKGLTSWPGKLVREDDvhNSCQQSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRK 1573
Cdd:cd05836      1 FKIGDLVWAKMKGFPPWPGKIVNPPP--DLKKPPRKKKMHCVYFFGSENYAWIEDENIKPYEEFKEEMLKSKK 71

PWWP domain found in Arabidopsis thaliana histone-lysine N-methyltransferase trithorax-like proteins ATX1, ATX2, and similar proteins; This family includes A. thaliana ATX1 and ATX2, which are sister paralogs originating from a segmental chromosomal duplication. They are plant counterparts of the Drosophila melanogaster trithorax (TRX) and mammalian mixed-lineage leukemia (MLL1) proteins. ATX1, also called protein SET domain group 27, or trithorax-homolog protein 1 (TRX-homolog protein 1), is a methyltransferase that trimethylates histone H3 at lysine 4 (H3K4me3). It also acts as a histone modifier and as a positive effector of gene expression. ATX1 regulates transcription from diverse classes of genes implicated in biotic and abiotic stress responses. It is involved in dehydration stress signaling in both abscisic acid (ABA)-dependent and ABA-independent pathways. ATX2, also called protein SET domain group 30, or trithorax-homolog protein 2 (TRX-homolog protein 2), is involved in dimethylating histone H3 at lysine 4 (H3K4me2). Both ATX1 and ATX2 are multi-domain containing proteins that consist of an N-terminal PWWP domain, FYRN- and FYRC (DAST, domain associated with SET in trithorax) domains, a canonical plant homeodomain (PHD) domain, a non-canonical extended PHD (ePHD) domain, and a C-terminal SET domain. The PWWP domain specifically recognizes DNA and histone methylated lysines.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2462575169 1502 NVGDLVWGQIKGLTSWPGKLVRED---DVHNSCQQSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEA-YSRVRKRNR 1576
Cdd:cd20142      1 SPGDVVWAKVKGYPMWPALVIDEEhaeRCGLEANRPGKKGTVPVQFFGTYEVARLNPKKVVGFSKGLDLkYHSKCKAPV 79

PWWP domain found in PWWP domain-containing protein 2B (PWWP2B); PWWP2B is a paralog of PWWP2A, a H2A.Z-specific chromatin binding protein which may play an important role in the neural crest stem cell migration and differentiation during early development. PWWP2A and PWWP2B form a stable complex with NuRD subunits MTA1/2/3:HDAC1/2:RBBP4/7, but not with MBD2/3, p66alpha/beta, and CHD3/4. The PWWP domain specifically recognizes DNA and histone methylated lysines.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462575169 1499 RTFNVGDLVWGQIKGLTSWPGKLV-----REDDVHNSCQQSPeegkvwVMWFGLHTFTQVEPEKLKTLTEgleaYSRVRK 1573
Cdd:cd20153     12 RTVSVGDIVWGKIHGFPWWPARVLsislsQKEDGEPSWQEAK------VSWFGSPTTSLLSVSKLSPFSE----FFKLRF 81

                           90       100       110
                   ....*....|....*....|....*....|....
gi 2462575169 1574 RNRKSG----------KLNNHLEAAIHEAMSELD 1597
Cdd:cd20153     82 NRKKKGmyrkaiteaaKAAEHLTPEIRELLTQFE 115

PWWP domain found in hepatoma-derived growth factor (HDGF)-related protein (HRP) family; The HRP family includes hepatoma-derived growth factor (HDGF), and HDGF-related proteins (HRPs). HDGF, also called high mobility group protein 1-like 2 (HMG-1L2), is a heparin-binding protein that acts as a transcriptional repressor with mitogenic activity for fibroblasts. It is a prognostic factor in several types of cancer. HDGFL1 is also called PWWP domain-containing protein 1 (PWWP1). Its biological function remains unclear. HDGFL2, also called HDGF-related protein 2 (HRP-2), or hepatoma-derived growth factor 2 (HDGF-2), is involved in cellular growth control, through the regulation of cyclin D1 expression. HDGFL3, also called HDGF-related protein 3 (HRP-3), enhances DNA synthesis and may play a role in cell proliferation. The family also includes PC4 and SFRS1-interacting protein (PSIP) and similar proteins. PSIP, also called CLL-associated antigen KW-7, dense fine speckles 70 kDa protein (DFS 70), lens epithelium-derived growth factor (LEDGF), or transcriptional coactivator p75/p52, acts as a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Members of the HRP family contains a PWWP domain, which is necessary for DNA binding.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2462575169 1501 FNVGDLVWGQIKGLTSWPGKLvreDDVHNscQQSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNR 1576
Cdd:cd05834      1 FKPGDLVFAKVKGYPPWPARI---DEIPE--GAKIPKNKYPVFFYGTHETAFLKPKDLFPYEENKEKYGKPRKRKG 71