SAM (Sterile alpha motif ); SAM (Sterile Alpha Motif) domain is a module consisting of approximately 70 amino acids. This domain is found in the Fungi/Metazoa group and in a restricted number of bacteria. Proteins with SAM domains are represented by a wide variety of domain architectures and have different intracellular localization, including nucleus, cytoplasm and membranes. SAM domains have diverse functions. They can interact with proteins, RNAs and membrane lipids, contain site of phosphorylation and/or kinase docking site, and play a role in protein homo and hetero dimerization/oligomerization in processes ranging from signal transduction to regulation of transcription. Mutations in SAM domains have been linked to several diseases.

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gi 2462619395  72 KPVALWTQQDVCKWLKKHCPNQYQIYR----------RALLRLTDKKLERMGIAQENLRQHILQQVLQLKVREEV 136
Cdd:cd09510     1 KPVYLWSVQDVCKWLKRHCPDYYLLYAelflqhditgRALLRLNDNKLERMGITDEDHRQDILREILKLRLKTEI 75

SAM domain of aveugle-like subfamily; SAM (sterile alpha motif) domain of SAM_aveugle-like subfamily is a protein-protein interaction domain. In Drosophila, the aveugle (AVE) protein (also known as HYP (Hyphen)) is involved in normal photoreceptor differentiation, and required for epidermal growth factor receptor (EGFR) signaling between ras and raf genes during eye development and wing vein formation. SAM domain of the HYP(AVE) protein interacts with SAM domain of CNK, the multidomain scaffold protein connector enhancer of kinase suppressor of ras. CNK/HYP(AVE) complex interacts with KSR (kinase suppressor of Ras) protein. This interaction leads to stimulation of Ras-dependent Raf activation. This subfamily also includes vertebrate AVE homologs - Samd10 and Samd12 proteins. Their exact function is unknown, but they may play a role in signal transduction during embryogenesis.

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gi 2462619395  72 KPVALWTQQDVCKWLKKHCPNQYQIYR----------RALLRLTDKKLERMGIAQENLRQHILQQVLQLKVREEV 136
Cdd:cd09510     1 KPVYLWSVQDVCKWLKRHCPDYYLLYAelflqhditgRALLRLNDNKLERMGITDEDHRQDILREILKLRLKTEI 75

SAM domain of aveugle-like subfamily; SAM (sterile alpha motif) domain of SAM_aveugle-like subfamily is a protein-protein interaction domain. In Drosophila, the aveugle (AVE) protein (also known as HYP (Hyphen)) is involved in normal photoreceptor differentiation, and required for epidermal growth factor receptor (EGFR) signaling between ras and raf genes during eye development and wing vein formation. SAM domain of the HYP(AVE) protein interacts with SAM domain of CNK, the multidomain scaffold protein connector enhancer of kinase suppressor of ras. CNK/HYP(AVE) complex interacts with KSR (kinase suppressor of Ras) protein. This interaction leads to stimulation of Ras-dependent Raf activation. This subfamily also includes vertebrate AVE homologs - Samd10 and Samd12 proteins. Their exact function is unknown, but they may play a role in signal transduction during embryogenesis.

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                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2462619395  72 KPVALWTQQDVCKWLKKHCPNQYQIYR----------RALLRLTDKKLERMGIAQENLRQHILQQVLQLKVREEV 136
Cdd:cd09510     1 KPVYLWSVQDVCKWLKRHCPDYYLLYAelflqhditgRALLRLNDNKLERMGITDEDHRQDILREILKLRLKTEI 75

SAM domain (Sterile alpha motif); It has been suggested that SAM is an evolutionarily conserved protein binding domain that is involved in the regulation of numerous developmental processes in diverse eukaryotes. The SAM domain can potentially function as a protein interaction module through its ability to homo- and heterooligomerise with other SAM domains.

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gi 2462619395  77 WTQQDVCKWLKKHCPNQY-------QIYRRALLRLTDKKLERMGIAQENLRQHILQQVLQLK 131
Cdd:pfam00536   3 WSVEDVGEWLESIGLGQYidsfragYIDGDALLQLTEDDLLKLGVTLLGHRKKILYAIQRLK 64

SAM domain of SAM_Neurabin-like subfamily; SAM (sterile alpha motif) domain of Neurabin-like (Neural actin-binding) subfamily is a putative protein-protein interaction domain. This group currently includes the SAM domains of neurobin-I, SAMD14 and neurobin-I/SAMD14-like proteins. Most are multidomain proteins and in addition to SAM domain they contain other protein-binding domains such as PDZ and actin-binding domains. Members of this subfamily participate in signal transduction. Neurabin-I is involved in the regulation of Ca signaling intensity in alpha-adrenergic receptors; it forms a functional pair of opposing regulators with neurabin-II. Neurabins are expressed almost exclusively in neuronal cells. They are known to interact with protein phosphatase 1 and inhibit its activity; they also can bind actin filaments; however, the exact role of the SAM domain is unclear, since SAM doesn't participate in these interactions.

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gi 2462619395  71 SKPVALWTQQDVCKWL-----KKHCP--NQYQIYRRALLRLTDKKLERMGIAQENLRQHILQQVLQLK 131
Cdd:cd09512     1 SRPVSEWSVQQVCQWLmglglEQYIPefTANNIDGQQLLQLDSSKLKALGITSSSDRSLLKKKLKELK 68