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Conserved domains on  [gi|2462525618|ref|XP_054224958|]
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solute carrier family 22 member 18 isoform X5 [Homo sapiens]

Protein Classification

MFS transporter( domain architecture ID 999995)

major facilitator superfamily (MFS) transporter facilitates the transport across cytoplasmic or internal membranes of one or more from a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides

CATH:  1.20.1250.20
Gene Ontology:  GO:0022857|GO:0055085
PubMed:  26758938|26098515
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MFS super family cl28910
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 55-270 3.37e-71

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


The actual alignment was detected with superfamily member cd17331:

Pssm-ID: 475125 [Multi-domain]  Cd Length: 382  Bit Score: 224.02  E-value: 3.37e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  55 SFTCIPASTKGAKTDAQAPLPGGPraSVFDLKAIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAG 134
Cdd:cd17331   169 VILLIPSNTKSKREKEAKESASPP--SVFNLKEILRLLRLPGVAFLLLVKLLSGLPIGMFQSMFSLVAMDFFGLTPEENG 246

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 135 YLMSFFGLLQMVTQGLVIGQLSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAV 214
Cdd:cd17331   247 YLMSYAGVLGMVVQGLLVGPLTKRFGERSLLKLSVALLAIGYLLLAFVTTVWQLLVLLLPLVIGGSVLYTVITSLLTKAV 326

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2462525618 215 STSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVPVFGHVQVAINTLVLLVLWR 270
Cdd:cd17331   327 PPEDTGTALGLSHALFSLVRTVSPTIGGALLQAYGFPSFGLLGAAINGLLTVYLLL 382
 
Name Accession Description Interval E-value
MFS_SLC22A18 cd17331
Solute carrier family 22 member 18 of the Major Facilitator Superfamily of transporters; ...
 55-270 3.37e-71

Solute carrier family 22 member 18 of the Major Facilitator Superfamily of transporters; Solute carrier family 22 member 18 (SLC22A18) is also called Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene A protein (BWR1A or BWSCR1A), efflux transporter-like protein, imprinted multi-membrane-spanning polyspecific transporter-related protein 1 (IMPT1), organic cation transporter-like protein 2 (ORCTL2), or tumor-suppressing subchromosomal transferable fragment candidate gene 5 protein (TSSC5). It is localized at the apical membrane surface of renal proximal tubules and may act as an organic cation/proton antiporter. It functions as a tumor suppressor in several cancer types including glioblastoma and colorectal cancer. SLC22A18 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340889 [Multi-domain]  Cd Length: 382  Bit Score: 224.02  E-value: 3.37e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  55 SFTCIPASTKGAKTDAQAPLPGGPraSVFDLKAIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAG 134
Cdd:cd17331   169 VILLIPSNTKSKREKEAKESASPP--SVFNLKEILRLLRLPGVAFLLLVKLLSGLPIGMFQSMFSLVAMDFFGLTPEENG 246

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 135 YLMSFFGLLQMVTQGLVIGQLSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAV 214
Cdd:cd17331   247 YLMSYAGVLGMVVQGLLVGPLTKRFGERSLLKLSVALLAIGYLLLAFVTTVWQLLVLLLPLVIGGSVLYTVITSLLTKAV 326

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2462525618 215 STSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVPVFGHVQVAINTLVLLVLWR 270
Cdd:cd17331   327 PPEDTGTALGLSHALFSLVRTVSPTIGGALLQAYGFPSFGLLGAAINGLLTVYLLL 382
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
78-230 2.65e-07

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 51.13  E-value: 2.65e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  78 PRASVFDLKAIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTQgLVIGQLSS 157
Cdd:COG2814   190 PAARARLRGSLRELLRRPRLLLLLLLAFLLGFGFFALFTYLPLYLQEVLGLSASAAGLLLALFGLGGVLGA-LLAGRLAD 268

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2462525618 158 HFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQ 230
Cdd:COG2814   269 RFGRRRLLLIGLLLLALGLLLLALAGSLWLLLLALFLLGFGFGLLFPLLQALVAELAPPEARGRASGLYNSAF 341
MFS_1 pfam07690
Major Facilitator Superfamily;
100-281 4.55e-06

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 47.41  E-value: 4.55e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 100 IFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTQgLVIGQLSSHFSEEVLLRASVLVFIVVGLAM 179
Cdd:pfam07690   1 LFLAAFLAALGRSLLGPALPLLLAEDLGISPTEIGLLLTLFSLGYALAQ-PLAGRLSDRFGRRRVLLIGLLLFALGLLLL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 180 ACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVPVFGHVQVA 259
Cdd:pfam07690  80 LFASSLWLLLVLRVLQGLGAGALFPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLLGGLLASLFGWRAAFLILAI 159

                         170       180
                  ....*....|....*....|..
gi 2462525618 260 INTLVLLVLWRKPMPQRKDKVR 281
Cdd:pfam07690 160 LSLLAAVLLLLPRPPPESKRPK 181
2A0115 TIGR00895
benzoate transport; [Transport and binding proteins, Carbohydrates, organic alcohols, and ...
 126-270 7.30e-03

benzoate transport; [Transport and binding proteins, Carbohydrates, organic alcohols, and acids]


Pssm-ID: 273327 [Multi-domain]  Cd Length: 398  Bit Score: 37.34  E-value: 7.30e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 126 FQLEAAQAGYLMSFfGLLQMVTQGLVIGQLSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLL--VPGLVFSLCTLN 203
Cdd:TIGR00895  47 WGLDPVQLGFLFSA-GLIGMAFGALFFGPLADRIGRRRVLLWSILLFSVFTLLCALATNVTQLLILrfLAGLGLGGLMPN 125

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2462525618 204 VVTdsMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVPVFGHVQVAINTLVLLVLWR 270
Cdd:TIGR00895 126 LNA--LVSEYAPKRFRGTAVGLMFCGYPIGAAVGGFLAGWLIPVFGWRSLFYVGGIAPLLLLLLLMR 190
 
Name Accession Description Interval E-value
MFS_SLC22A18 cd17331
Solute carrier family 22 member 18 of the Major Facilitator Superfamily of transporters; ...
 55-270 3.37e-71

Solute carrier family 22 member 18 of the Major Facilitator Superfamily of transporters; Solute carrier family 22 member 18 (SLC22A18) is also called Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene A protein (BWR1A or BWSCR1A), efflux transporter-like protein, imprinted multi-membrane-spanning polyspecific transporter-related protein 1 (IMPT1), organic cation transporter-like protein 2 (ORCTL2), or tumor-suppressing subchromosomal transferable fragment candidate gene 5 protein (TSSC5). It is localized at the apical membrane surface of renal proximal tubules and may act as an organic cation/proton antiporter. It functions as a tumor suppressor in several cancer types including glioblastoma and colorectal cancer. SLC22A18 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340889 [Multi-domain]  Cd Length: 382  Bit Score: 224.02  E-value: 3.37e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  55 SFTCIPASTKGAKTDAQAPLPGGPraSVFDLKAIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAG 134
Cdd:cd17331   169 VILLIPSNTKSKREKEAKESASPP--SVFNLKEILRLLRLPGVAFLLLVKLLSGLPIGMFQSMFSLVAMDFFGLTPEENG 246

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 135 YLMSFFGLLQMVTQGLVIGQLSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAV 214
Cdd:cd17331   247 YLMSYAGVLGMVVQGLLVGPLTKRFGERSLLKLSVALLAIGYLLLAFVTTVWQLLVLLLPLVIGGSVLYTVITSLLTKAV 326

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2462525618 215 STSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVPVFGHVQVAINTLVLLVLWR 270
Cdd:cd17331   327 PPEDTGTALGLSHALFSLVRTVSPTIGGALLQAYGFPSFGLLGAAINGLLTVYLLL 382
MFS_SLC46_TetA_like cd17330
Eukaryotic Solute carrier 46 (SLC46) family, Bacterial Tetracycline resistance proteins, and ...
 87-270 4.47e-30

Eukaryotic Solute carrier 46 (SLC46) family, Bacterial Tetracycline resistance proteins, and similar proteins of the Major Facilitator Superfamily of transporters; This family is composed of the eukaryotic proteins MFSD9, MFSD10, MFSD14, and SLC46 family proteins, as well as bacterial multidrug resistance (MDR) transporters such as tetracycline resistance protein TetA and multidrug resistance protein MdtG. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. TetA proteins confer resistance to tetracycline while MdtG confers resistance to fosfomycin and deoxycholate. The Solute carrier 46 (SLC46) family is composed of three vertebrate members (SLC46A1, SLC46A2, and SLC46A3), the best-studied of which is SLC46A1, which functions both as an intestinal proton-coupled high-affinity folate transporter involved in the absorption of folates and as an intestinal heme transporter which mediates heme uptake. MFSD10 facilitates the uptake of organic anions such as some non-steroidal anti-inflammatory drugs (NSAIDs) and confers resistance to such NSAIDs. The SLC46/TetA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340888 [Multi-domain]  Cd Length: 349  Bit Score: 115.75  E-value: 4.47e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  87 AIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTQGLVIGQLSSHFSEEVLLR 166
Cdd:cd17330   166 LVLVLFFLPPLRRLLLVYFLIALAFAGLESVFSLFLKDRFGFSPSQIGLLLALVGIAGILAQLLLIPRLAKRLGERKLLR 245

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 167 ASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYR 246
Cdd:cd17330   246 LGLLLAIIGLLLLPFASSVPLLVLAAAILAFGTGIVFPALSALLSKSVPSKEQGKAQGIIQSLQSLARIIGPLLAGPLYS 325

                         170       180
                  ....*....|....*....|....
gi 2462525618 247 SFGVPVFGHVQVAINTLVLLVLWR 270
Cdd:cd17330   326 FLGPGVPFLLAAGLLLIALFLLSL 349
MFS_MFSD9 cd17390
Major facilitator superfamily domain-containing protein 9; Major facilitator superfamily ...
 80-272 5.18e-17

Major facilitator superfamily domain-containing protein 9; Major facilitator superfamily domain-containing protein 9 (MFSD9) is expressed in the central nervous system (CNS) and in most peripheral tissues but at very low expression levels. The function of MFSD9 is unknown. MFSD9 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340948 [Multi-domain]  Cd Length: 350  Bit Score: 79.58  E-value: 5.18e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  80 ASVFDLKAIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTQGLV--IGQLSS 157
Cdd:cd17390   157 SAIFILNAGLVWFLLSKLWDLLLVRFLMGFSVLLFRSNFSLYLEERFDLSPKTIGYLISYSGVLGALSGFLVgrILKLYK 236

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 158 HFSEEVLLRASVLVFIVVgLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTLG 237
Cdd:cd17390   237 NNEYLLLLHSFILLALSL-LGLALAPSIWLLVLCLTPLSISSAVLRTCSTSLLLSRGKSKERGTLLGVGQSVTSLARILA 315

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 2462525618 238 PTVGGLLYrSFGVPVFGHVQVAINTLVLLVLWRKP 272
Cdd:cd17390   316 PLLSGLLQ-EFSVSGPGLLGAVLALAAILLMLARQ 349
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
 75-251 5.93e-10

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 59.13  E-value: 5.93e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  75 PGGPRASVFDLKAIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTQGLViGQ 154
Cdd:cd17325   179 NKRAVSAARLRSGLRLLLRDRRLLALFLAIFVLAFAFGALEPFLPLYAAELGGLSPAQIGLLFGAQGLASALSQPPA-GK 257

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 155 LSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLR 234
Cdd:cd17325   258 LSDRIGRKPLILIGLLLSAVALLLLPLATSFWLLLLLLALLGLGLGLVFPATLALLADIVPPEGRGTAMGLFNTAFSLGM 337

                         170
                  ....*....|....*..
gi 2462525618 235 TLGPTVGGLLYRSFGVP 251
Cdd:cd17325   338 VLGPLLGGFLYDAFGFA 354
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
78-230 2.65e-07

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 51.13  E-value: 2.65e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  78 PRASVFDLKAIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTQgLVIGQLSS 157
Cdd:COG2814   190 PAARARLRGSLRELLRRPRLLLLLLLAFLLGFGFFALFTYLPLYLQEVLGLSASAAGLLLALFGLGGVLGA-LLAGRLAD 268

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2462525618 158 HFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQ 230
Cdd:COG2814   269 RFGRRRLLLIGLLLLALGLLLLALAGSLWLLLLALFLLGFGFGLLFPLLQALVAELAPPEARGRASGLYNSAF 341
MFS_1 pfam07690
Major Facilitator Superfamily;
100-281 4.55e-06

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 47.41  E-value: 4.55e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 100 IFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTQgLVIGQLSSHFSEEVLLRASVLVFIVVGLAM 179
Cdd:pfam07690   1 LFLAAFLAALGRSLLGPALPLLLAEDLGISPTEIGLLLTLFSLGYALAQ-PLAGRLSDRFGRRRVLLIGLLLFALGLLLL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 180 ACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVPVFGHVQVA 259
Cdd:pfam07690  80 LFASSLWLLLVLRVLQGLGAGALFPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLLGGLLASLFGWRAAFLILAI 159

                         170       180
                  ....*....|....*....|..
gi 2462525618 260 INTLVLLVLWRKPMPQRKDKVR 281
Cdd:pfam07690 160 LSLLAAVLLLLPRPPPESKRPK 181
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
111-270 1.01e-05

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 46.12  E-value: 1.01e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 111 TGLFMVMFSIISMDFfQLEAAQAGYLMSFFGLLQMVTQgLVIGQLSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHFCL 190
Cdd:COG2814    26 IGIVLPALPLIAADL-GASPAQAGLVVTAYLLGAALGA-PLAGRLADRFGRRRVLLLGLLLFALGSLLCALAPSLWLLLA 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 191 LVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVPVFGHVQVAINTLVLLVLWR 270
Cdd:COG2814   104 ARFLQGLGAGALFPAALALIADLVPPERRGRALGLLGAGLGLGPALGPLLGGLLADLFGWRWVFLVNAVLALLALLLLLR 183
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 78-245 3.72e-05

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 44.72  E-value: 3.72e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  78 PRASVFDLKAIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTqGLVIGQLSS 157
Cdd:cd06174   186 EASSKSVLKLLKRVLKNPGLWLLLLAIFLVNLAYYSFSTLLPLFLLDLGGLSVAVAGLLLSLFGLAGALG-SLLLGLLSD 264

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 158 HFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLLVPGLV-FSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTL 236
Cdd:cd06174   265 RLIGRKPLLLIGLLLMALGLALLLLAPSLLLLLLLLLLLgFGLGGLLPLSFALIAELFPPEIRGTAFGLLNTFGFLGGAI 344


                  ....*....
gi 2462525618 237 GPTVGGLLY 245
Cdd:cd06174   345 GPLLAGFLL 353
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 123-281 5.28e-05

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 43.95  E-value: 5.28e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 123 MDFFQLEAAQAGYLMSFFGLLQMVTQgLVIGQLSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTL 202
Cdd:cd06174    23 LQSFGLSASQLGLLFALFSLGYALLQ-PLAGLLADRFGRRPVLLLGLLLFALGALLFAFAPSFWLLLLGRFLLGLGSGLI 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 203 NVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVPVFGHVQV-AINTLVLLVLWRKPMPQRKDKVR 281
Cdd:cd06174   102 DPAVLALIADLFPERERGRALGLLQAFGSVGGILGPLLGGILASSLGFGWRAVFLIaAALALLAAILLLLVVPDPPESAR 181
MFS_MefA_like cd06173
Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of ...
 70-271 1.47e-04

Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of transporters; This family is composed of Streptococcus pyogenes macrolide efflux protein A (MefA) and similar transporters, many of which remain uncharacterized. Some members may be multidrug resistance (MDR) transporters, which are drug/H+ antiporters (DHAs) that mediate the efflux of a variety of drugs and toxic compounds, conferring resistance to these compounds. MefA confers resistance to 14-membered macrolides including erythromycin and to 15-membered macrolides. It functions as an efflux pump to regulate intracellular macrolide levels. The MefA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340863 [Multi-domain]  Cd Length: 383  Bit Score: 42.60  E-value: 1.47e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  70 AQAPLPGGPRASVFDLKAIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGlLQMVTQG 149
Cdd:cd06173   183 PAAPGESSSLLLRDLREGLRYLRRSPLLRLLLLALALFALLGGALTVLLPLLAKEVLGGGAAGYGLLLAAFG-VGALLGA 261

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 150 LVIGQLSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASV 229
Cdd:cd06173   262 LLLGRLSKRRRRGRLLLIGALLLGLALLVLGLSPSLWLLLAALFLLGLAGGLFNVPLNTLLQLRVPDELRGRVFSVYNAL 341

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 2462525618 230 QPLLRTLGPTVGGLLYRSFGVPVFGHVQVAINTLVLLVLWRK 271
Cdd:cd06173   342 NSGAMPLGALLAGLLADALGLSAVFLLAGALLLLAALLLLLL 383
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
87-277 5.35e-04

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 41.01  E-value: 5.35e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  87 AIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTqGLVIGQLSSHFSEE--VL 164
Cdd:COG2271   172 LPGLLLALLRFWLLALAYFLVYFALYGFLTWLPTYLVEVRGLSLAQAGLLLSLPFLAGIVG-SLLGGWLSDRLGRRrkLV 250

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 165 LRASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLL 244
Cdd:COG2271   251 LAIGLLLAALALLLLALLPSPALAIALLFLAGFGLGGAFGLLWALAAELFPKKARGTASGLVNTFGFLGGALGPLLVGYL 330

                         170       180       190
                  ....*....|....*....|....*....|...
gi 2462525618 245 YRSFGVPVFGHVQVAINTLVLLVLWRKPMPQRK 277
Cdd:COG2271   331 LDATGYQAAFLLLAALALLAALLALLLLRETRK 363
NarK COG2223
Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];
75-277 7.31e-04

Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];


Pssm-ID: 441825 [Multi-domain]  Cd Length: 392  Bit Score: 40.63  E-value: 7.31e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618  75 PGGPRASVFDLKAIASLLRLPDVPRIFLVKVASNCPTGLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTqGLVIGQ 154
Cdd:COG2223   185 AGAAAAAKASLRDQLEALRDPRFWLLSLLYFGTFGSFIGFSSWLPPYLVDQFGLSAATAGLLAALFALLGALG-RPLGGW 263

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 155 LSSHFSEEVLLrasVLVFIVVGLAMACMSSVFH--FCLLVPGLVFSLCT--LNVVTDSMLIKAVSTSDTGTMLGLCASVQ 230
Cdd:COG2223   264 LSDRIGGRRVL---LIVFALMALGLLLLALALGslWLFLVLFLLLGLALggGNGAVFALVPDIFPTKNAGAVYGLVGAAG 340

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 2462525618 231 PLLRTLGPTVGGLLYRSFGVPVFG-HVQVAINTLVLLVLWRKPMPQRK 277
Cdd:COG2223   341 GLGGFLGPLLFGALLDATGSYTAAfLVFAVLALVALVLTLLLYRRPRR 388
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
 130-281 1.30e-03

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 39.87  E-value: 1.30e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 130 AAQAGYLMSFFGLLQMVTQgLVIGQLSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLLVPGLVFSLCTLNVVTDSM 209
Cdd:cd17325    30 VAQIGLIVAAYALAQLLFS-PPAGRLSDRFGRKRLLLLGLLLLAVSTLLFAFATSYWQLLLARFLQGLASAAVWPAAMAL 108

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2462525618 210 LIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVP----VFGHVQVAINTLVLLVLWRKPMPQRKDKVR 281
Cdd:cd17325   109 IADIAPPEERGRAMGIFSSAIGLGFLIGPLLGGLLADALGYRapflVCAALALLALVLALLLLPEPRPPPNKRAVS 184
MFS_MFSD8 cd17326
Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) ...
 117-271 2.10e-03

Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) domain-containing protein 8 (MFSD8) is also called ceroid-lipofuscinosis neuronal protein 7 (CLN7). It is a polytopic lysosomal membrane protein that may transport small solutes by using chemiosmotic ion gradients. Mutations in MFSD8/CLN7 cause a variant of late-infantile neuronal ceroid lipofuscinoses (vLINCL), a neurodegenerative lysosomal storage disorder. Some variants are associated with nonsyndromic autosomal recessive macular dystrophy. MFSD8/CLN7 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340884 [Multi-domain]  Cd Length: 374  Bit Score: 39.11  E-value: 2.10e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 117 MFSIISMDFFQLEAAQA----GYLMSFFGLLQMVTQGLVIGQLSSHFSEEVLLRASVLVFIVVGLamacMSSVFHFCL-L 191
Cdd:cd17326   216 IGTPLTMDMYGWTREEAvlynGIILAVSGVLSVLVFLLFKFLSLGLIDERKILLGGLILFFLGFV----VTLPWGITGvI 291

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 192 VPGLVFSLCtlNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFG-VPVFGH--VQVAINTLVLLVL 268
Cdd:cd17326   292 LFGLGYPIG--NVMLYTLYSKILGPKPQGTMQGVLTASGSLARTLGPIFVTAIYTHFGpRWTWIItlGLLLLAIILLIVF 369


                  ...
gi 2462525618 269 WRK 271
Cdd:cd17326   370 YKR 372
MFS_YfmO_like cd17474
Bacillus subtilis multidrug efflux protein YfmO and similar transporters of the Major ...
 112-270 5.75e-03

Bacillus subtilis multidrug efflux protein YfmO and similar transporters of the Major Facilitator Superfamily; This family is composed of Bacillus subtilis multidrug efflux protein YfmO, bacillibactin exporter YmfD/YmfE, uncharacterized MFS-type transporter YvmA, and similar proteins. YfmO acts to efflux copper or a copper complex, and could contribute to copper resistance. YmfD/YmfE is involved in secretion of bacillibactin. The YfmO-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341027 [Multi-domain]  Cd Length: 374  Bit Score: 37.94  E-value: 5.75e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 112 GLFMVMFSIISMDFFQLEAAQAGYLMSFFGLLQMVTQGLVIGQLSSHFSEEVLLRASVLVFivvGLAMACMSSVFHFCLL 191
Cdd:cd17474   215 FAFLTYLPFLLELLYGISPIIIGLLFAGWSVLLALGSLFVGRLLKKRFGARRILVIGLLLY---ALSLLLLGFVPSLALL 291

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 192 VPGLVFS---LCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVPVFGHVQVAINTLVLLVL 268
Cdd:cd17474   292 LIAIVLFglgLGLNLPLLTTLVTELAPEEERGTASSLYNFVRFLGQAVGPLLFGLLLEIFGLSAPFLVGAAIALAAAVLL 371


                  ..
gi 2462525618 269 WR 270
Cdd:cd17474   372 AL 373
2A0115 TIGR00895
benzoate transport; [Transport and binding proteins, Carbohydrates, organic alcohols, and ...
 126-270 7.30e-03

benzoate transport; [Transport and binding proteins, Carbohydrates, organic alcohols, and acids]


Pssm-ID: 273327 [Multi-domain]  Cd Length: 398  Bit Score: 37.34  E-value: 7.30e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 126 FQLEAAQAGYLMSFfGLLQMVTQGLVIGQLSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHFCLL--VPGLVFSLCTLN 203
Cdd:TIGR00895  47 WGLDPVQLGFLFSA-GLIGMAFGALFFGPLADRIGRRRVLLWSILLFSVFTLLCALATNVTQLLILrfLAGLGLGGLMPN 125

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2462525618 204 VVTdsMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFGVPVFGHVQVAINTLVLLVLWR 270
Cdd:TIGR00895 126 LNA--LVSEYAPKRFRGTAVGLMFCGYPIGAAVGGFLAGWLIPVFGWRSLFYVGGIAPLLLLLLLMR 190
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
112-249 9.99e-03

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 37.16  E-value: 9.99e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462525618 112 GLFMVMFSIIS---MDFFQLEAAQAGYLMSFFGLLQMVTQgLVIGQLSSHFSEEVLLRASVLVFIVVGLAMACMSSVFHF 188
Cdd:COG2271    23 YLDRSNLSVAApplQADLGLSAAQLGLLASAFFLGYALGQ-IPAGLLADRFGRRRVLAIGLLLWGLATLLFGFATSFWQL 101

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2462525618 189 CLLVPGLVFSLCTLNVVTDSMLIKAVSTSDTGTMLGLCASVQPLLRTLGPTVGGLLYRSFG 249
Cdd:COG2271   102 LLLRFLLGLGEAGFFPAALKLIAEWFPPKERGRALGIFNAGGPLGGALAPPLLGWLLAAFG 162
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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