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Conserved domains on  [gi|76150625|ref|NP_001028886|]
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probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase isoform 1 [Homo sapiens]

Protein Classification

NOL1/NOP2/SUN domain family protein( domain architecture ID 1003105)

NOL1/NOP2/SUN domain (NSUN) family protein such as mammalian nucleolar protein NOP2 which is required for nucleolar maturation and ribosome biogenesis

Gene Ontology:  GO:0003723|GO:0000027|GO:0008173|GO:0001510

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
nop2p super family cl33194
NOL1/NOP2/sun family putative RNA methylase; [Protein synthesis, tRNA and rRNA base ...
 308-582 8.25e-117

NOL1/NOP2/sun family putative RNA methylase; [Protein synthesis, tRNA and rRNA base modification]


The actual alignment was detected with superfamily member TIGR00446:

Pssm-ID: 188051 [Multi-domain]  Cd Length: 264  Bit Score: 354.08  E-value: 8.25e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   308 LRTNTLKTRRRDLAQALINRGVNLDPLGKWSKtgLVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDM 387
Cdd:TIGR00446   1 IRVNTLKINVEDLLERLENRGVVLEPTCEEYF--FEVKESPFSIGSTPEYLFGYYYPQEASSMIPPIALEPREDERVLDM 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   388 CCAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISK 467
Cdd:TIGR00446  79 AAAPGGKTTQISQLMKNKGCIVANEISKSRTKALISNINRMGVLNTIVINADGRKFGAYLLKFDAILLDAPCSGEGVIRK 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   468 DPAVKTNKDEKDILRCAHLQKELLLSAIDSVnatsKTGGYLVYCTCSITVEENEWVVDYALKKR-NVRLVPTgldFGQEG 546
Cdd:TIGR00446 159 DPSRKRNWSEEDIKYCSLLQKELIDAAIDAL----KPGGVLVYSTCSLEVEENEEVIDYILRKRpDVVEEII---KGDEF 231

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 76150625   547 FTRFRERrfhPSLRSTRRFYPHTHNMDGFFIAKFKK 582
Cdd:TIGR00446 232 FGINIGK---GEVKGALRVFPQNYDCEGFFVAKLRK 264
 
Name Accession Description Interval E-value
nop2p TIGR00446
NOL1/NOP2/sun family putative RNA methylase; [Protein synthesis, tRNA and rRNA base ...
 308-582 8.25e-117

NOL1/NOP2/sun family putative RNA methylase; [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 188051 [Multi-domain]  Cd Length: 264  Bit Score: 354.08  E-value: 8.25e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   308 LRTNTLKTRRRDLAQALINRGVNLDPLGKWSKtgLVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDM 387
Cdd:TIGR00446   1 IRVNTLKINVEDLLERLENRGVVLEPTCEEYF--FEVKESPFSIGSTPEYLFGYYYPQEASSMIPPIALEPREDERVLDM 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   388 CCAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISK 467
Cdd:TIGR00446  79 AAAPGGKTTQISQLMKNKGCIVANEISKSRTKALISNINRMGVLNTIVINADGRKFGAYLLKFDAILLDAPCSGEGVIRK 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   468 DPAVKTNKDEKDILRCAHLQKELLLSAIDSVnatsKTGGYLVYCTCSITVEENEWVVDYALKKR-NVRLVPTgldFGQEG 546
Cdd:TIGR00446 159 DPSRKRNWSEEDIKYCSLLQKELIDAAIDAL----KPGGVLVYSTCSLEVEENEEVIDYILRKRpDVVEEII---KGDEF 231

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 76150625   547 FTRFRERrfhPSLRSTRRFYPHTHNMDGFFIAKFKK 582
Cdd:TIGR00446 232 FGINIGK---GEVKGALRVFPQNYDCEGFFVAKLRK 264
RsmB COG0144
16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF family [Translation, ribosomal structure and ...
 233-582 4.30e-108

16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF family [Translation, ribosomal structure and biogenesis]; 16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF family is part of the Pathway/BioSystem: 16S rRNA modification


Pssm-ID: 439914 [Multi-domain]  Cd Length: 441  Bit Score: 338.14  E-value: 4.30e-108
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625 233 QRVHKRIQDIV-GILRDFGAQREEGRSRSeylnRLKKDLAIYYSYGDFLLGKLMDLFPLSELVEFLEANEVPRPVTLRTN 311
Cdd:COG0144 107 EAVGPGAAGLVnAVLRRVLREREALLAAA----DPVERLAVRYSHPEWLVERLRAAYGPEEAEALLAALNEPPPLDLRVN 182

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625 312 TLKTRRRDLAQALINRGVNLDPlGKWSKTGLVVyDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAP 391
Cdd:COG0144 183 TLKASREELLARLAEEGIEAEP-TPLSPDGLRL-EGPGPVTALPGFREGLFSVQDEASQLVALLLDPKPGERVLDLCAAP 260

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625 392 GGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGG-FDRVLLDAPCSGTGVISKDPA 470
Cdd:COG0144 261 GGKTLHLAELMGNKGRVVAVDISEHRLKRLRENLARLGLSNVEVVVADARELLEWLPGkFDRVLLDAPCSGTGTLRRHPD 340

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625 471 VKTNKDEKDILRCAHLQKELLLSAIDSVnatsKTGGYLVYCTCSITVEENEWVVDYALKKR-NVRLVPTGLDFGQEGFTR 549
Cdd:COG0144 341 IKWRRTPEDIAELAALQRELLDAAARLL----KPGGRLVYSTCSLLPEENEEVVEAFLARHpDFELVPLAELLPGLALTD 416

                       330       340       350
                ....*....|....*....|....*....|...
gi 76150625 550 frerrfhpslRSTRRFYPHTHNMDGFFIAKFKK 582
Cdd:COG0144 417 ----------GGYLRLLPHRHGTDGFFIARLRK 439
Methyltr_RsmB-F pfam01189
16S rRNA methyltransferase RsmB/F; This is the catalytic core of this SAM-dependent 16S ...
 373-581 9.09e-104

16S rRNA methyltransferase RsmB/F; This is the catalytic core of this SAM-dependent 16S ribosomal methyltransferase RsmB/F enzyme. There is a catalytic cysteine residue at 180 in UniProtKB:Q5SII2, with another highly conserved cysteine at residue 230. It methylates the C(5) position of cytosine 2870 (m5C2870) in 25S rRNA.


Pssm-ID: 426109 [Multi-domain]  Cd Length: 199  Bit Score: 317.83  E-value: 9.09e-104
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   373 VMALAPQEHERILDMCCAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGG--F 450
Cdd:pfam01189   1 AILLAPQEGETILDMCAAPGGKTTHIAELMKNQGTVVAVDINKHRLKRVAENIHRLGVTNTIILNGDGRQPDQWLGGvlF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   451 DRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHLQKELLLSAIDSVnatsKTGGYLVYCTCSITVEENEWVVDYALKK 530
Cdd:pfam01189  81 DRILLDAPCSGTGVIRRHPDVKWLRQEADIAQLAQLQKELLSAAIDLL----KPGGVLVYSTCSVLPEENEAVIEYFLQK 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 76150625   531 R-NVRLVPT------GLDFGQEgftrfrerrfhpslrSTRRFYPHTHNMDGFFIAKFK 581
Cdd:pfam01189 157 HpDVELVPTplfepvGLAIGEQ---------------PTLRLLPHTHNGDGFFIAKLR 199
PRK14902 PRK14902
16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB;
234-582 3.21e-86

16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB;


Pssm-ID: 237857 [Multi-domain]  Cd Length: 444  Bit Score: 280.91  E-value: 3.21e-86
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  234 RVHKRIQDIV-GILRDFgaQREEGRSRSEyLNRLKKDLAIYYSYGDFLLGKLMDLFPLSELVEFLEANEVPRPVTLRTNT 312
Cdd:PRK14902 109 RGHKGIAKFVnGVLRNI--LREGLPDIDE-IKDPVKRLSIKYSHPVWLVKRWIDQYGEEKAEKILESLNEPPKASIRVNT 185

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  313 LKTRRRDLAQALINRGVNLDPlGKWSKTGLVVydSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPG 392
Cdd:PRK14902 186 LKISVEELIEKLEEEGYEVEE-SLLSPEALVI--EKGNIAGTDLFKDGLITIQDESSMLVAPALDPKGGDTVLDACAAPG 262

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  393 GKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGG-FDRVLLDAPCSGTGVISKDPAV 471
Cdd:PRK14902 263 GKTTHIAELLKNTGKVVALDIHEHKLKLIEENAKRLGLTNIETKALDARKVHEKFAEkFDKILVDAPCSGLGVIRRKPDI 342

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  472 KTNKDEKDILRCAHLQKELLlsaiDSVNATSKTGGYLVYCTCSITVEENEWVVDYALKKrnvrlvptGLDFGQEGFTRFR 551
Cdd:PRK14902 343 KYNKTKEDIESLQEIQLEIL----ESVAQYLKKGGILVYSTCTIEKEENEEVIEAFLEE--------HPEFELVPLQHEK 410

                        330       340       350
                 ....*....|....*....|....*....|..
gi 76150625  552 -ERRFHPSLRSTRRFYPHTHNMDGFFIAKFKK 582
Cdd:PRK14902 411 pDELVYEVKDGYLQILPNDYGTDGFFIAKLRK 442
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 383-511 1.67e-05

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 44.34  E-value: 1.67e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625 383 RILDMCCAPGGKTSYMAQLMKntGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKV-VGGFDRVLLDAPCsg 461
Cdd:cd02440   1 RVLDLGCGTGALALALASGPG--ARVTGVDISPVALELARKAAAALLADNVEVLKGDAEELPPEaDESFDVIISDPPL-- 76

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|
gi 76150625 462 tgviskdpavktnkdekdilrcaHLQKELLLSAIDSVNATSKTGGYLVYC 511
Cdd:cd02440  77 -----------------------HHLVEDLARFLEEARRLLKPGGVLVLT 103
 
Name Accession Description Interval E-value
nop2p TIGR00446
NOL1/NOP2/sun family putative RNA methylase; [Protein synthesis, tRNA and rRNA base ...
 308-582 8.25e-117

NOL1/NOP2/sun family putative RNA methylase; [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 188051 [Multi-domain]  Cd Length: 264  Bit Score: 354.08  E-value: 8.25e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   308 LRTNTLKTRRRDLAQALINRGVNLDPLGKWSKtgLVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDM 387
Cdd:TIGR00446   1 IRVNTLKINVEDLLERLENRGVVLEPTCEEYF--FEVKESPFSIGSTPEYLFGYYYPQEASSMIPPIALEPREDERVLDM 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   388 CCAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISK 467
Cdd:TIGR00446  79 AAAPGGKTTQISQLMKNKGCIVANEISKSRTKALISNINRMGVLNTIVINADGRKFGAYLLKFDAILLDAPCSGEGVIRK 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   468 DPAVKTNKDEKDILRCAHLQKELLLSAIDSVnatsKTGGYLVYCTCSITVEENEWVVDYALKKR-NVRLVPTgldFGQEG 546
Cdd:TIGR00446 159 DPSRKRNWSEEDIKYCSLLQKELIDAAIDAL----KPGGVLVYSTCSLEVEENEEVIDYILRKRpDVVEEII---KGDEF 231

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 76150625   547 FTRFRERrfhPSLRSTRRFYPHTHNMDGFFIAKFKK 582
Cdd:TIGR00446 232 FGINIGK---GEVKGALRVFPQNYDCEGFFVAKLRK 264
RsmB COG0144
16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF family [Translation, ribosomal structure and ...
 233-582 4.30e-108

16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF family [Translation, ribosomal structure and biogenesis]; 16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF family is part of the Pathway/BioSystem: 16S rRNA modification


Pssm-ID: 439914 [Multi-domain]  Cd Length: 441  Bit Score: 338.14  E-value: 4.30e-108
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625 233 QRVHKRIQDIV-GILRDFGAQREEGRSRSeylnRLKKDLAIYYSYGDFLLGKLMDLFPLSELVEFLEANEVPRPVTLRTN 311
Cdd:COG0144 107 EAVGPGAAGLVnAVLRRVLREREALLAAA----DPVERLAVRYSHPEWLVERLRAAYGPEEAEALLAALNEPPPLDLRVN 182

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625 312 TLKTRRRDLAQALINRGVNLDPlGKWSKTGLVVyDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAP 391
Cdd:COG0144 183 TLKASREELLARLAEEGIEAEP-TPLSPDGLRL-EGPGPVTALPGFREGLFSVQDEASQLVALLLDPKPGERVLDLCAAP 260

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625 392 GGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGG-FDRVLLDAPCSGTGVISKDPA 470
Cdd:COG0144 261 GGKTLHLAELMGNKGRVVAVDISEHRLKRLRENLARLGLSNVEVVVADARELLEWLPGkFDRVLLDAPCSGTGTLRRHPD 340

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625 471 VKTNKDEKDILRCAHLQKELLLSAIDSVnatsKTGGYLVYCTCSITVEENEWVVDYALKKR-NVRLVPTGLDFGQEGFTR 549
Cdd:COG0144 341 IKWRRTPEDIAELAALQRELLDAAARLL----KPGGRLVYSTCSLLPEENEEVVEAFLARHpDFELVPLAELLPGLALTD 416

                       330       340       350
                ....*....|....*....|....*....|...
gi 76150625 550 frerrfhpslRSTRRFYPHTHNMDGFFIAKFKK 582
Cdd:COG0144 417 ----------GGYLRLLPHRHGTDGFFIARLRK 439
Methyltr_RsmB-F pfam01189
16S rRNA methyltransferase RsmB/F; This is the catalytic core of this SAM-dependent 16S ...
 373-581 9.09e-104

16S rRNA methyltransferase RsmB/F; This is the catalytic core of this SAM-dependent 16S ribosomal methyltransferase RsmB/F enzyme. There is a catalytic cysteine residue at 180 in UniProtKB:Q5SII2, with another highly conserved cysteine at residue 230. It methylates the C(5) position of cytosine 2870 (m5C2870) in 25S rRNA.


Pssm-ID: 426109 [Multi-domain]  Cd Length: 199  Bit Score: 317.83  E-value: 9.09e-104
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   373 VMALAPQEHERILDMCCAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGG--F 450
Cdd:pfam01189   1 AILLAPQEGETILDMCAAPGGKTTHIAELMKNQGTVVAVDINKHRLKRVAENIHRLGVTNTIILNGDGRQPDQWLGGvlF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   451 DRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHLQKELLLSAIDSVnatsKTGGYLVYCTCSITVEENEWVVDYALKK 530
Cdd:pfam01189  81 DRILLDAPCSGTGVIRRHPDVKWLRQEADIAQLAQLQKELLSAAIDLL----KPGGVLVYSTCSVLPEENEAVIEYFLQK 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 76150625   531 R-NVRLVPT------GLDFGQEgftrfrerrfhpslrSTRRFYPHTHNMDGFFIAKFK 581
Cdd:pfam01189 157 HpDVELVPTplfepvGLAIGEQ---------------PTLRLLPHTHNGDGFFIAKLR 199
PRK14902 PRK14902
16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB;
234-582 3.21e-86

16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB;


Pssm-ID: 237857 [Multi-domain]  Cd Length: 444  Bit Score: 280.91  E-value: 3.21e-86
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  234 RVHKRIQDIV-GILRDFgaQREEGRSRSEyLNRLKKDLAIYYSYGDFLLGKLMDLFPLSELVEFLEANEVPRPVTLRTNT 312
Cdd:PRK14902 109 RGHKGIAKFVnGVLRNI--LREGLPDIDE-IKDPVKRLSIKYSHPVWLVKRWIDQYGEEKAEKILESLNEPPKASIRVNT 185

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  313 LKTRRRDLAQALINRGVNLDPlGKWSKTGLVVydSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPG 392
Cdd:PRK14902 186 LKISVEELIEKLEEEGYEVEE-SLLSPEALVI--EKGNIAGTDLFKDGLITIQDESSMLVAPALDPKGGDTVLDACAAPG 262

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  393 GKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGG-FDRVLLDAPCSGTGVISKDPAV 471
Cdd:PRK14902 263 GKTTHIAELLKNTGKVVALDIHEHKLKLIEENAKRLGLTNIETKALDARKVHEKFAEkFDKILVDAPCSGLGVIRRKPDI 342

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  472 KTNKDEKDILRCAHLQKELLlsaiDSVNATSKTGGYLVYCTCSITVEENEWVVDYALKKrnvrlvptGLDFGQEGFTRFR 551
Cdd:PRK14902 343 KYNKTKEDIESLQEIQLEIL----ESVAQYLKKGGILVYSTCTIEKEENEEVIEAFLEE--------HPEFELVPLQHEK 410

                        330       340       350
                 ....*....|....*....|....*....|..
gi 76150625  552 -ERRFHPSLRSTRRFYPHTHNMDGFFIAKFKK 582
Cdd:PRK14902 411 pDELVYEVKDGYLQILPNDYGTDGFFIAKLRK 442
yebU PRK11933
rRNA (cytosine-C(5)-)-methyltransferase RsmF; Reviewed
 295-588 2.42e-59

rRNA (cytosine-C(5)-)-methyltransferase RsmF; Reviewed


Pssm-ID: 183387 [Multi-domain]  Cd Length: 470  Bit Score: 208.99  E-value: 2.42e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  295 EFLEANEVPRPVTLRTNTLKTRRRDLAQALINRGVNLDPLgKWSKTGLVV---YDSSVPIGATPEYLAGHYMLQGASSML 371
Cdd:PRK11933  24 DFIAACQRPLRRSIRVNTLKISVADFLQLMAPYGWTLTPI-PWCEEGFWIerdDEDALPLGNTAEHLSGLFYIQEASSML 102

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  372 PVMAL-APQEH-ERILDMCCAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFpkvvGG 449
Cdd:PRK11933 103 PVAALfADDNApQRVLDMAAAPGSKTTQIAALMNNQGAIVANEYSASRVKVLHANISRCGVSNVALTHFDGRVF----GA 178

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  450 -----FDRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHLQKELLLSAIDSVnatsKTGGYLVYCTCSITVEENEWVV 524
Cdd:PRK11933 179 alpetFDAILLDAPCSGEGTVRKDPDALKNWSPESNLEIAATQRELIESAFHAL----KPGGTLVYSTCTLNREENQAVC 254

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 76150625  525 DYaLKKR---NVRLVPTGLDFGQ-------EGFtrfrerrFHpslrstrrFYPHTHNMDGFFIAKFKKfSNSIP 588
Cdd:PRK11933 255 LW-LKETypdAVEFEPLGDLFPGaekalteEGF-------LH--------VFPQIYDSEGFFVARLRK-TASVP 311
PRK14901 PRK14901
16S rRNA methyltransferase B; Provisional
 244-582 1.32e-53

16S rRNA methyltransferase B; Provisional


Pssm-ID: 237856 [Multi-domain]  Cd Length: 434  Bit Score: 192.07  E-value: 1.32e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  244 GILRDFGAQREEGRSRSEYLNRLKKdLAIYYSYGDFLLGKLMDLFPLSELVEFLEA-NEVPrPVTLRTNTLKTRRRDLAQ 322
Cdd:PRK14901 118 GILRQYLRAREAGDPLPLPEDPIER-LAILHSFPDWLVKLWLEWLGLEEAEQLCKWfNQPP-SLDLRINPLRTSLEEVQA 195

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  323 ALINRGVNLDPLGKwSKTGLVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYMAQLM 402
Cdd:PRK14901 196 ALAEAGITATPIPG-LPQGLRLTGNPGSIRQLPGYEEGWWTVQDRSAQLVAPLLDPQPGEVILDACAAPGGKTTHIAELM 274

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  403 KNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQF----PKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEK 478
Cdd:PRK14901 275 GDQGEIWAVDRSASRLKKLQENAQRLGLKSIKILAADSRNLlelkPQWRGYFDRILLDAPCSGLGTLHRHPDARWRQTPE 354

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  479 DILRCAHLQKELLLSAIDSVnatsKTGGYLVYCTCSITVEENEWVVDyalkkrnvrlvptgldfgqegftRFRERrfHPS 558
Cdd:PRK14901 355 KIQELAPLQAELLESLAPLL----KPGGTLVYATCTLHPAENEAQIE-----------------------QFLAR--HPD 405

                        330       340
                 ....*....|....*....|....*..
gi 76150625  559 LRS---TRRFYPHTHNMDGFFIAKFKK 582
Cdd:PRK14901 406 WKLeppKQKIWPHRQDGDGFFMAVLRK 432
rsmB TIGR00563
16S rRNA (cytosine(967)-C(5))-methyltransferase; This protein is also known as sun protein. ...
 236-582 2.14e-53

16S rRNA (cytosine(967)-C(5))-methyltransferase; This protein is also known as sun protein. The reading frame was originally interpreted as two reading frames, fmu and fmv. The recombinant protein from E. coli was shown to methylate only C967 of small subunit (16S) ribosomal RNA and to produce only m5C at that position. The seed alignment is built from bacterial sequences only. Eukaryotic homologs include Nop2, a protein required for processing pre-rRNA, that is likely also a rRNA methyltransferase, although the fine specificity may differ. Cutoff scores are set to avoid treating archaeal and eukaroytic homologs automatically as functionally equivalent, although they may have very similar roles. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 273141 [Multi-domain]  Cd Length: 426  Bit Score: 191.23  E-value: 2.14e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   236 HKRIQDIV-GILRDFgaQREEgrsrsEYLNRLKKDLAIYYSYGDFLLGKLMDLFPLSElVEFLEANEVPRPVTLRTNTLK 314
Cdd:TIGR00563 103 RKGLKGLVnGVLRRF--QREQ-----EELLAEFNALDARYLHPEWLVKRLQKAYPGQW-QSICEANNQRPPMWLRINRTK 174

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   315 TRRRDLAQALINRGVNLDPLGKWSKTglVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGK 394
Cdd:TIGR00563 175 HSRDEWLALLAEAGMKGFPHDLAPDA--VRLETPAAVHALPGFEEGWVTVQDASAQWVATWLAPQNEETILDACAAPGGK 252

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   395 TSYMAQLMKNtGVILANDANAERLKSVVGNLHRLGVTnTIISHYDGRQ----FPKVVGGFDRVLLDAPCSGTGVISKDPA 470
Cdd:TIGR00563 253 TTHILELAPQ-AQVVALDIHEHRLKRVYENLKRLGLT-IKAETKDGDGrgpsQWAENEQFDRILLDAPCSATGVIRRHPD 330

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625   471 VKTNKDEKDILRCAHLQKELLlsaiDSVNATSKTGGYLVYCTCSITVEENEWVVDyalkkrnvrlvptglDFGQEGFTR- 549
Cdd:TIGR00563 331 IKWLRKPRDIAELAELQSEIL----DAIWPLLKTGGTLVYATCSVLPEENSEQIK---------------AFLQEHPDFp 391

                         330       340       350
                  ....*....|....*....|....*....|....
gi 76150625   550 FRERRFHPSLRSTR-RFYPHTHNMDGFFIAKFKK 582
Cdd:TIGR00563 392 FEKTGTPEQVRDGGlQILPHAEEGDGFFYAKLIK 425
PRK10901 PRK10901
16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB;
244-582 5.73e-52

16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB;


Pssm-ID: 236790 [Multi-domain]  Cd Length: 427  Bit Score: 187.32  E-value: 5.73e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  244 GILRDFgaQREegrsRSEYLNRLKKDLAIYYSYGDFLLGKLMDLFPlSELVEFLEANEVPRPVTLRTNTLKTRRRDLAQA 323
Cdd:PRK10901 117 AVLRRF--QRE----QEELLAELQADPVARYNHPSWLIKRLKKAYP-EQWQAILAANNQRPPMWLRVNRRHHSRDAYLAL 189

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  324 LINRGVNLDPlGKWSKTGLVVyDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYMAQLMK 403
Cdd:PRK10901 190 LAEAGIEAFP-HAVGPDAIRL-ETPVPVHQLPGFAEGWVSVQDAAAQLAATLLAPQNGERVLDACAAPGGKTAHILELAP 267

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  404 NTGVIlANDANAERLKSVVGNLHRLGVTNTII--------SHYDGRQfpkvvggFDRVLLDAPCSGTGVISKDPAVKTNK 475
Cdd:PRK10901 268 QAQVV-ALDIDAQRLERVRENLQRLGLKATVIvgdardpaQWWDGQP-------FDRILLDAPCSATGVIRRHPDIKWLR 339

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  476 DEKDILRCAHLQKELLlsaiDSVNATSKTGGYLVYCTCSITVEENEWVVDYALKKR-NVRLVPTGLDFGqegftrfrerr 554
Cdd:PRK10901 340 RPEDIAALAALQSEIL----DALWPLLKPGGTLLYATCSILPEENEQQIKAFLARHpDAELLDTGTPQQ----------- 404

                        330       340
                 ....*....|....*....|....*...
gi 76150625  555 fhpslrSTRRFYPHTHNMDGFFIAKFKK 582
Cdd:PRK10901 405 ------PGRQLLPGEEDGDGFFYALLIK 426
PRK14903 PRK14903
16S rRNA methyltransferase B; Provisional
 268-538 8.72e-37

16S rRNA methyltransferase B; Provisional


Pssm-ID: 184896 [Multi-domain]  Cd Length: 431  Bit Score: 143.86  E-value: 8.72e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  268 KDLAIYYSYGDFLLGKLMDLFPLSELVEFLEANEVPRPVTLRTNTLKTRRRDLAQALINRGVNLDPlGKWSKTGLVVYDS 347
Cdd:PRK14903 126 KELHLKYSHPKWLVNYWRSFLPEEAVLRIMEWNQEPLPTMLRVNSLAITREEVIKILAEEGTEAVP-GKHSPFSLIVRKL 204

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  348 SVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHR 427
Cdd:PRK14903 205 GVNMNDSRVIKDGLATVQGESSQIVPLLMELEPGLRVLDTCAAPGGKTTAIAELMKDQGKILAVDISREKIQLVEKHAKR 284

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  428 LGVTNTIISHYDGRQFPKVVGG-FDRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHLQKELLLSAIDSVnatsKTGG 506
Cdd:PRK14903 285 LKLSSIEIKIADAERLTEYVQDtFDRILVDAPCTSLGTARNHPEVLRRVNKEDFKKLSEIQLRIVSQAWKLL----EKGG 360

                        250       260       270
                 ....*....|....*....|....*....|...
gi 76150625  507 YLVYCTCSITVEENEWVV-DYALKKRNVRLVPT 538
Cdd:PRK14903 361 ILLYSTCTVTKEENTEVVkRFVYEQKDAEVIDI 393
PRK14904 PRK14904
16S rRNA methyltransferase B; Provisional
 231-530 2.68e-34

16S rRNA methyltransferase B; Provisional


Pssm-ID: 237858 [Multi-domain]  Cd Length: 445  Bit Score: 136.73  E-value: 2.68e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  231 DLQRVHK--RIQDIV-GILRDFGAQREEGRSRSEYLNRLKKdLAIYYSYGDFLLGKLMDLFPLSELVEFLEANEVPRPVT 307
Cdd:PRK14904 104 KLARKYKgeHMAKLVnGVLRNISPETISLDEWLKGMPEAER-LSLLYSHPEWLLERWIARYGEERTEAMLSYNNQAPLFG 182

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  308 LRTNTLKTRRRDLAQALINRGVnldplgKWSKTGLVVYDSSVPIGATPEYLA-GHYMLQGASSMLPVMALAPQEHERILD 386
Cdd:PRK14904 183 FRINRLKTTPEKFLAAPADASV------TFEKSGLPNFFLSKDFSLFEPFLKlGLVSVQNPTQALACLLLNPQPGSTVLD 256

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625  387 MCCAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVtnTIIS--HYDGRQFPKvVGGFDRVLLDAPCSGTGV 464
Cdd:PRK14904 257 LCAAPGGKSTFMAELMQNRGQITAVDRYPQKLEKIRSHASALGI--TIIEtiEGDARSFSP-EEQPDAILLDAPCTGTGV 333

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 76150625  465 ISKDPAVKTNKDEKDILRCAHLQKELLlsaiDSVNATSKTGGYLVYCTCSITVEENEWVVDYALKK 530
Cdd:PRK14904 334 LGRRAELRWKLTPEKLAELVGLQAELL----DHAASLLKPGGVLVYATCSIEPEENELQIEAFLQR 395
Methyltr_RsmF_N pfam17125
N-terminal domain of 16S rRNA methyltransferase RsmF; This is the N-terminal domain of the ...
 291-369 6.09e-10

N-terminal domain of 16S rRNA methyltransferase RsmF; This is the N-terminal domain of the RsmF methyl transferase. RsmF is a multi-site-specific methyltransferase that is responsible for the synthesis of three modifications on cytidines in 16S ribosomal RNA. The N-terminus is critical for stabilising the catalytic core of the enzyme.


Pssm-ID: 465356 [Multi-domain]  Cd Length: 88  Bit Score: 56.33  E-value: 6.09e-10
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 76150625   291 SELVEFLEANEVPRPVTLRTNTLKTRRRDLAQALinrGVNLDPLgKWSKTGLvVYDSSVPIGATPEYLAGHYMLQGASS 369
Cdd:pfam17125  15 DEYEAFLAAYDEPRPVGLRVNTLKISVEEFLKLS---PFELEPV-PWCEEGF-YLEERPRPGKHPYHHAGLYYIQEPSA 88
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 383-511 1.67e-05

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 44.34  E-value: 1.67e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 76150625 383 RILDMCCAPGGKTSYMAQLMKntGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKV-VGGFDRVLLDAPCsg 461
Cdd:cd02440   1 RVLDLGCGTGALALALASGPG--ARVTGVDISPVALELARKAAAALLADNVEVLKGDAEELPPEaDESFDVIISDPPL-- 76

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|
gi 76150625 462 tgviskdpavktnkdekdilrcaHLQKELLLSAIDSVNATSKTGGYLVYC 511
Cdd:cd02440  77 -----------------------HHLVEDLARFLEEARRLLKPGGVLVLT 103
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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