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Conserved domains on  [gi|110225353|ref|NP_001035987|]
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sprouty-related, EVH1 domain-containing protein 3 isoform 1 [Homo sapiens]

Protein Classification

sprouty-related, EVH1 domain-containing protein( domain architecture ID 10180423)

sprouty-related, EVH1 domain-containing protein is a tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase, and may negatively regulate Ras signaling pathways

Gene Ontology:  GO:0043409|GO:0019901
PubMed:  11911879

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
EVH1_SPRED-like cd10574
Sprouty-related EVH1 domain-containing-like proteins EVH1 domain; The Spred family has the ...
2-105 1.18e-47

Sprouty-related EVH1 domain-containing-like proteins EVH1 domain; The Spred family has the following domains: an N-terminal EVH1 domain, a unique KBD (c-Kit kinase binding) domain which that is phosphorylated by the stem cell factor receptor c-Kit, and a C-terminal cysteine-rich SPR (Sprouty-related) domain which is involved in membrane localization. There are 3 Spred proteins: Spred1 which interacts with both Ras and Raf through its SPR domain; Spred2 which is the most abundant isoform; and Spred3 which has a non-functional KBD and maintains the inhibitory action on Raf. Legius syndrome is caused by heterozygous mutations in Spred1. Both EVH1 and SPR domains are involved in the inhibition of the MAP kinase pathway by Spred proteins. The specific function of the Spred2 EVH1 domain is unknown and there are no known interacting proteins to date. It is thought that its EVH1 domain will have a fourth distinct peptide binding mechanism within the EVH1 family. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


:

Pssm-ID: 269978  Cd Length: 113  Bit Score: 158.25  E-value: 1.18e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110225353   2 VRVRAVVMARDDSSGGWLPVGGGGLSQVSVCRVRgarPEGGARQGHYVIHGERLRDQKTTLECTLKPGLVYNKVNPIFHH 81
Cdd:cd10574    3 VRVRAVVMTRDDSSGGWLPLGGGGLSIVSVCKVM---PEEGAPRTEYVIHGERIRDKTVVLECTLRKDLVYNKVMPTFHH 79
                         90       100
                 ....*....|....*....|....
gi 110225353  82 WSLGDCKFGLTFQSPAEADEFQKS 105
Cdd:cd10574   80 WRIGEKKFGLTFQSPADARAFDRG 103
Sprouty pfam05210
Sprouty protein (Spry); This family consists of eukaryotic Sprouty protein homologs. Sprouty ...
296-400 3.60e-20

Sprouty protein (Spry); This family consists of eukaryotic Sprouty protein homologs. Sprouty proteins have been revealed as inhibitors of the Ras/mitogen-activated protein kinase (MAPK) cascade, a pathway crucial for developmental processes initiated by activation of various receptor tyrosine kinases. The sprouty gene has found to be expressed in the the brain, cochlea, nasal organs, teeth, salivary gland, lungs, digestive tract, kidneys and limb buds in mice.


:

Pssm-ID: 461589  Cd Length: 101  Bit Score: 84.69  E-value: 3.60e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110225353  296 RCVHCRALFRRRADGRGGRCAEApdpgrlLVRRLSCLWCAESLLYHCLSDAEGDFS-DPCACePGHPRPAARWAALAALS 374
Cdd:pfam05210   3 RCVYCQELFTEPRNLPGSCSAES------CIDYVSCMCCAKGLFYHCMSDAEGDDScDPCSC-CSPHRCCKRWLALALLS 75
                          90       100
                  ....*....|....*....|....*.
gi 110225353  375 LAVPCLCCYAPLRACHWVAARCGCAG 400
Cdd:pfam05210  76 LLLPCLCCYPPLRACHKCCVACGCCG 101
 
Name Accession Description Interval E-value
EVH1_SPRED-like cd10574
Sprouty-related EVH1 domain-containing-like proteins EVH1 domain; The Spred family has the ...
2-105 1.18e-47

Sprouty-related EVH1 domain-containing-like proteins EVH1 domain; The Spred family has the following domains: an N-terminal EVH1 domain, a unique KBD (c-Kit kinase binding) domain which that is phosphorylated by the stem cell factor receptor c-Kit, and a C-terminal cysteine-rich SPR (Sprouty-related) domain which is involved in membrane localization. There are 3 Spred proteins: Spred1 which interacts with both Ras and Raf through its SPR domain; Spred2 which is the most abundant isoform; and Spred3 which has a non-functional KBD and maintains the inhibitory action on Raf. Legius syndrome is caused by heterozygous mutations in Spred1. Both EVH1 and SPR domains are involved in the inhibition of the MAP kinase pathway by Spred proteins. The specific function of the Spred2 EVH1 domain is unknown and there are no known interacting proteins to date. It is thought that its EVH1 domain will have a fourth distinct peptide binding mechanism within the EVH1 family. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269978  Cd Length: 113  Bit Score: 158.25  E-value: 1.18e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110225353   2 VRVRAVVMARDDSSGGWLPVGGGGLSQVSVCRVRgarPEGGARQGHYVIHGERLRDQKTTLECTLKPGLVYNKVNPIFHH 81
Cdd:cd10574    3 VRVRAVVMTRDDSSGGWLPLGGGGLSIVSVCKVM---PEEGAPRTEYVIHGERIRDKTVVLECTLRKDLVYNKVMPTFHH 79
                         90       100
                 ....*....|....*....|....
gi 110225353  82 WSLGDCKFGLTFQSPAEADEFQKS 105
Cdd:cd10574   80 WRIGEKKFGLTFQSPADARAFDRG 103
Sprouty pfam05210
Sprouty protein (Spry); This family consists of eukaryotic Sprouty protein homologs. Sprouty ...
296-400 3.60e-20

Sprouty protein (Spry); This family consists of eukaryotic Sprouty protein homologs. Sprouty proteins have been revealed as inhibitors of the Ras/mitogen-activated protein kinase (MAPK) cascade, a pathway crucial for developmental processes initiated by activation of various receptor tyrosine kinases. The sprouty gene has found to be expressed in the the brain, cochlea, nasal organs, teeth, salivary gland, lungs, digestive tract, kidneys and limb buds in mice.


Pssm-ID: 461589  Cd Length: 101  Bit Score: 84.69  E-value: 3.60e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110225353  296 RCVHCRALFRRRADGRGGRCAEApdpgrlLVRRLSCLWCAESLLYHCLSDAEGDFS-DPCACePGHPRPAARWAALAALS 374
Cdd:pfam05210   3 RCVYCQELFTEPRNLPGSCSAES------CIDYVSCMCCAKGLFYHCMSDAEGDDScDPCSC-CSPHRCCKRWLALALLS 75
                          90       100
                  ....*....|....*....|....*.
gi 110225353  375 LAVPCLCCYAPLRACHWVAARCGCAG 400
Cdd:pfam05210  76 LLLPCLCCYPPLRACHKCCVACGCCG 101
WH1 smart00461
WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains ...
4-105 4.52e-10

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.


Pssm-ID: 214674  Cd Length: 106  Bit Score: 56.60  E-value: 4.52e-10
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110225353     4 VRAVVMARDDSSGGWLPVGGGGLSQVsVCRVRgarpeggARQGHYVIHGERLRDQkTTLECTLKPGLVYNKVNPIFHHWS 83
Cdd:smart00461   9 ARAVVQLYDADTKKWVPTGEGGAANL-VIDKN-------QRSYFFRIVGIKGQDK-VIWNQELYKNFKYNQATPTFHQWA 79
                           90       100
                   ....*....|....*....|..
gi 110225353    84 LGDCKFGLTFQSPAEADEFQKS 105
Cdd:smart00461  80 DDKCVYGLNFASEEEAKKFRKK 101
WH1 pfam00568
WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in ...
48-104 1.05e-08

WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in Wiskott-Aldrich syndrome (WAS). The majority of point mutations occur within the amino- terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homolog. A subset of WH1 domains has been termed a "EVH1" domain and appear to bind a polyproline motif.


Pssm-ID: 395450  Cd Length: 111  Bit Score: 52.84  E-value: 1.05e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 110225353   48 YVIHGERLRDQKTTLECTLKPGLVYNKVNPIFHHWSLGDCKFGLTFQSPAEADEFQK 104
Cdd:pfam00568  49 YFIRLVDIQDGKVIWNQEIYPNMEYNQARPFFHTFADSRCVYGLNFASEEEATKFAK 105
 
Name Accession Description Interval E-value
EVH1_SPRED-like cd10574
Sprouty-related EVH1 domain-containing-like proteins EVH1 domain; The Spred family has the ...
2-105 1.18e-47

Sprouty-related EVH1 domain-containing-like proteins EVH1 domain; The Spred family has the following domains: an N-terminal EVH1 domain, a unique KBD (c-Kit kinase binding) domain which that is phosphorylated by the stem cell factor receptor c-Kit, and a C-terminal cysteine-rich SPR (Sprouty-related) domain which is involved in membrane localization. There are 3 Spred proteins: Spred1 which interacts with both Ras and Raf through its SPR domain; Spred2 which is the most abundant isoform; and Spred3 which has a non-functional KBD and maintains the inhibitory action on Raf. Legius syndrome is caused by heterozygous mutations in Spred1. Both EVH1 and SPR domains are involved in the inhibition of the MAP kinase pathway by Spred proteins. The specific function of the Spred2 EVH1 domain is unknown and there are no known interacting proteins to date. It is thought that its EVH1 domain will have a fourth distinct peptide binding mechanism within the EVH1 family. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269978  Cd Length: 113  Bit Score: 158.25  E-value: 1.18e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110225353   2 VRVRAVVMARDDSSGGWLPVGGGGLSQVSVCRVRgarPEGGARQGHYVIHGERLRDQKTTLECTLKPGLVYNKVNPIFHH 81
Cdd:cd10574    3 VRVRAVVMTRDDSSGGWLPLGGGGLSIVSVCKVM---PEEGAPRTEYVIHGERIRDKTVVLECTLRKDLVYNKVMPTFHH 79
                         90       100
                 ....*....|....*....|....
gi 110225353  82 WSLGDCKFGLTFQSPAEADEFQKS 105
Cdd:cd10574   80 WRIGEKKFGLTFQSPADARAFDRG 103
EVH1_family cd00837
EVH1 (Drosophila Enabled (Ena)/Vasodilator-stimulated phosphoprotein (VASP) homology 1) domain; ...
1-104 6.91e-28

EVH1 (Drosophila Enabled (Ena)/Vasodilator-stimulated phosphoprotein (VASP) homology 1) domain; The EVH1 domains are part of the PH domain superfamily. EVH1 subfamilies include Enables/VASP, Homer/Vesl, WASP, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269909  Cd Length: 103  Bit Score: 106.01  E-value: 6.91e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110225353   1 MVRVRAVVMARDDSSGGWLPVGGGGLSQVSVCRVRGarpeggarQGHYVIHGERLRDQKTTLECTLKPGLVYNKVNPIFH 80
Cdd:cd00837    2 IFSARAHVMQIDDSNKNWVPAGGKGASRVSYFKDTT--------RNSFRIIGVDIKDKKVVINCTITKNLVYNKATQTFH 73
                         90       100
                 ....*....|....*....|....
gi 110225353  81 HWSLGDCKFGLTFQSPAEADEFQK 104
Cdd:cd00837   74 QWADDRTVFGLNFASEEDATKFAE 97
Sprouty pfam05210
Sprouty protein (Spry); This family consists of eukaryotic Sprouty protein homologs. Sprouty ...
296-400 3.60e-20

Sprouty protein (Spry); This family consists of eukaryotic Sprouty protein homologs. Sprouty proteins have been revealed as inhibitors of the Ras/mitogen-activated protein kinase (MAPK) cascade, a pathway crucial for developmental processes initiated by activation of various receptor tyrosine kinases. The sprouty gene has found to be expressed in the the brain, cochlea, nasal organs, teeth, salivary gland, lungs, digestive tract, kidneys and limb buds in mice.


Pssm-ID: 461589  Cd Length: 101  Bit Score: 84.69  E-value: 3.60e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110225353  296 RCVHCRALFRRRADGRGGRCAEApdpgrlLVRRLSCLWCAESLLYHCLSDAEGDFS-DPCACePGHPRPAARWAALAALS 374
Cdd:pfam05210   3 RCVYCQELFTEPRNLPGSCSAES------CIDYVSCMCCAKGLFYHCMSDAEGDDScDPCSC-CSPHRCCKRWLALALLS 75
                          90       100
                  ....*....|....*....|....*.
gi 110225353  375 LAVPCLCCYAPLRACHWVAARCGCAG 400
Cdd:pfam05210  76 LLLPCLCCYPPLRACHKCCVACGCCG 101
WH1 smart00461
WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains ...
4-105 4.52e-10

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.


Pssm-ID: 214674  Cd Length: 106  Bit Score: 56.60  E-value: 4.52e-10
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110225353     4 VRAVVMARDDSSGGWLPVGGGGLSQVsVCRVRgarpeggARQGHYVIHGERLRDQkTTLECTLKPGLVYNKVNPIFHHWS 83
Cdd:smart00461   9 ARAVVQLYDADTKKWVPTGEGGAANL-VIDKN-------QRSYFFRIVGIKGQDK-VIWNQELYKNFKYNQATPTFHQWA 79
                           90       100
                   ....*....|....*....|..
gi 110225353    84 LGDCKFGLTFQSPAEADEFQKS 105
Cdd:smart00461  80 DDKCVYGLNFASEEEAKKFRKK 101
WH1 pfam00568
WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in ...
48-104 1.05e-08

WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in Wiskott-Aldrich syndrome (WAS). The majority of point mutations occur within the amino- terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homolog. A subset of WH1 domains has been termed a "EVH1" domain and appear to bind a polyproline motif.


Pssm-ID: 395450  Cd Length: 111  Bit Score: 52.84  E-value: 1.05e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 110225353   48 YVIHGERLRDQKTTLECTLKPGLVYNKVNPIFHHWSLGDCKFGLTFQSPAEADEFQK 104
Cdd:pfam00568  49 YFIRLVDIQDGKVIWNQEIYPNMEYNQARPFFHTFADSRCVYGLNFASEEEATKFAK 105
EVH1_Ena_VASP-like cd01207
Enabled/VASP family EVH1 domain; Ena/VASP family includes proteins such as: ...
4-102 6.67e-08

Enabled/VASP family EVH1 domain; Ena/VASP family includes proteins such as: Vasodilator-stimulated phosphoprotein (VASP), enabled gene product from Drosophila (Ena), mammalian enabled (Mena) and Ena/VASP-Like protein (EVL) localize to focal adhesions and to sites of actin filament dynamics. These proteins share a common modular organization with a highly conserved N- and C-terminal domains, termed Ena/VASP homology domains 1 and 2 (EVH1 and EVH2), that are separated by a central proline-rich domain. The EVH1 domain binds to other proteins at proline rich sequences. The majority of Ena-VASP type EVH1 domains recognize FPPPP motifs such as in the focal adhesion proteins zyxin and vinculin, and the ActA surface protein of Listeria monocytogenes, however the LIM3 domain of Tes lacks the FPPPP motif but still binds the EVH1 domain of Mena. It has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains. EVH2 mediates oligomerization within the family. The proline-rich region binds SH3 and WW domains as well as profilin, a protein that regulates actin filament dynamics. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269918  Cd Length: 108  Bit Score: 50.38  E-value: 6.67e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110225353   4 VRAVVMARDDSSGG-WLPVGGGGLSQVSV--CRVRGArpeggarqghYVIHGERLRDQKTTLECTLKPGLVYNKVNPIFH 80
Cdd:cd01207    5 ARASVMVYDDENKRwVPSGGSQGLSRVQIyhNTRNNT----------FRVVGRKLQDHEVVINCAILKGLKYNQATPTFH 74
                         90       100
                 ....*....|....*....|....
gi 110225353  81 HWSlgDCK--FGLTFQSPAEADEF 102
Cdd:cd01207   75 QWR--DARqvYGLNFASKEEATEF 96
EVH1_WASP-like cd01205
WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called ...
55-104 2.83e-03

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269916  Cd Length: 101  Bit Score: 37.12  E-value: 2.83e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 110225353  55 LRDQKTTLECTLKPGLVYNKVNPIFHHWSLGDCKFGLTFQSPAEADEFQK 104
Cdd:cd01205   46 LKTRGVVWEQELYEGFEYNQDRPFFHTFEGDECMIGLNFADEDEAAAFYK 95
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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