NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|194440700|ref|NP_001123951|]
View 

nucleobindin-2 precursor [Mus musculus]

Protein Classification

EF-hand domain-containing protein( domain architecture ID 12144783)

EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

CATH:  1.10.238.10
Gene Ontology:  GO:0005509
PubMed:  2479149|10191494
SCOP:  3001983

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
247-322 1.93e-05

EF-hand domain pair;


:

Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 42.24  E-value: 1.93e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 194440700  247 KTFFKLHDVNNDGFLDEQELEALFTRELEKVynpqnaeddmiemeEERLRMREHVMSEIDNNKDRLVTLEEFLRAT 322
Cdd:pfam13499   5 KEAFKLLDSDGDGYLDVEELKKLLRKLEEGE--------------PLSDEEVEELFKEFDLDKDGRISFEEFLELY 66
PRK12704 super family cl36166
phosphodiesterase; Provisional
 343-400 1.48e-04

phosphodiesterase; Provisional


The actual alignment was detected with superfamily member PRK12704:

Pssm-ID: 237177 [Multi-domain]  Cd Length: 520  Bit Score: 44.00  E-value: 1.48e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 194440700 343 TEDELKEYESIIAIQENELKKRAEELQKQKEDLQRQHDHLEAQKQEYHQAVQHLEQKK 400
Cdd:PRK12704  73 FEKELRERRNELQKLEKRLLQKEENLDRKLELLEKREEELEKKEKELEQKQQELEKKE 130
 
Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
247-322 1.93e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 42.24  E-value: 1.93e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 194440700  247 KTFFKLHDVNNDGFLDEQELEALFTRELEKVynpqnaeddmiemeEERLRMREHVMSEIDNNKDRLVTLEEFLRAT 322
Cdd:pfam13499   5 KEAFKLLDSDGDGYLDVEELKKLLRKLEEGE--------------PLSDEEVEELFKEFDLDKDGRISFEEFLELY 66
PRK12704 PRK12704
phosphodiesterase; Provisional
 343-400 1.48e-04

phosphodiesterase; Provisional


Pssm-ID: 237177 [Multi-domain]  Cd Length: 520  Bit Score: 44.00  E-value: 1.48e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 194440700 343 TEDELKEYESIIAIQENELKKRAEELQKQKEDLQRQHDHLEAQKQEYHQAVQHLEQKK 400
Cdd:PRK12704  73 FEKELRERRNELQKLEKRLLQKEENLDRKLELLEKREEELEKKEKELEQKQQELEKKE 130
CwlO1 COG3883
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 307-406 1.63e-03

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 40.58  E-value: 1.63e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194440700 307 NNKDRLVTLEEFLRATEKKEFLepDSWETLDQQQLFTEDELKEYESIIAIQEN---ELKKRAEELQKQKEDLQRQHDHLE 383
Cdd:COG3883   97 RSGGSVSYLDVLLGSESFSDFL--DRLSALSKIADADADLLEELKADKAELEAkkaELEAKLAELEALKAELEAAKAELE 174

                         90       100
                 ....*....|....*....|....*
gi 194440700 384 AQKQEYHQAVQHL--EQKKLQQGIA 406
Cdd:COG3883  175 AQQAEQEALLAQLsaEEAAAEAQLA 199
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
250-338 3.37e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 37.46  E-value: 3.37e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194440700 250 FKLHDVNNDGFLDEQELEALFTRELEKVYNPQNAEDDMIEMEEERLRMREHVMSE------------IDNNKDRLVTLEE 317
Cdd:COG5126   11 FDLLDADGDGVLERDDFEALFRRLWATLFSEADTDGDGRISREEFVAGMESLFEAtvepfaraafdlLDTDGDGKISADE 90

                         90       100
                 ....*....|....*....|.
gi 194440700 318 FLRATEKKEFLEPDSWETLDQ 338
Cdd:COG5126   91 FRRLLTALGVSEEEADELFAR 111
MAD pfam05557
Mitotic checkpoint protein; This family consists of several eukaryotic mitotic checkpoint ...
 294-399 4.56e-03

Mitotic checkpoint protein; This family consists of several eukaryotic mitotic checkpoint (Mitotic arrest deficient or MAD) proteins. The mitotic spindle checkpoint monitors proper attachment of the bipolar spindle to the kinetochores of aligned sister chromatids and causes a cell cycle arrest in prometaphase when failures occur. Multiple components of the mitotic spindle checkpoint have been identified in yeast and higher eukaryotes. In S.cerevisiae, the existence of a Mad1-dependent complex containing Mad2, Mad3, Bub3 and Cdc20 has been demonstrated.


Pssm-ID: 461677 [Multi-domain]  Cd Length: 660  Bit Score: 39.34  E-value: 4.56e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194440700  294 RLRMREHVMSE-----IDNNKDRLVTLEEFLRATEKKEFLEPDSWETldqqQLFTEDELKEYESIIAIQENELKkraeEL 368
Cdd:pfam05557  59 LLEKREAEAEEalreqAELNRLKKKYLEALNKKLNEKESQLADAREV----ISCLKNELSELRRQIQRAELELQ----ST 130

                          90       100       110
                  ....*....|....*....|....*....|.
gi 194440700  369 QKQKEDLQRQHDHLEAQKQEYHQAVQHLEQK 399
Cdd:pfam05557 131 NSELEELQERLDLLKAKASEAEQLRQNLEKQ 161
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 250-321 8.36e-03

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 34.83  E-value: 8.36e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 194440700 250 FKLHDVNNDGFLDEQELEALFTRELEKVYNPQnaeddmiemeeerlrmREHVMSEIDNNKDRLVTLEEFLRA 321
Cdd:cd00051    6 FRLFDKDGDGTISADELKAALKSLGEGLSEEE----------------IDEMIREVDKDGDGKIDFEEFLEL 61
 
Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
247-322 1.93e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 42.24  E-value: 1.93e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 194440700  247 KTFFKLHDVNNDGFLDEQELEALFTRELEKVynpqnaeddmiemeEERLRMREHVMSEIDNNKDRLVTLEEFLRAT 322
Cdd:pfam13499   5 KEAFKLLDSDGDGYLDVEELKKLLRKLEEGE--------------PLSDEEVEELFKEFDLDKDGRISFEEFLELY 66
PRK12704 PRK12704
phosphodiesterase; Provisional
 343-400 1.48e-04

phosphodiesterase; Provisional


Pssm-ID: 237177 [Multi-domain]  Cd Length: 520  Bit Score: 44.00  E-value: 1.48e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 194440700 343 TEDELKEYESIIAIQENELKKRAEELQKQKEDLQRQHDHLEAQKQEYHQAVQHLEQKK 400
Cdd:PRK12704  73 FEKELRERRNELQKLEKRLLQKEENLDRKLELLEKREEELEKKEKELEQKQQELEKKE 130
CwlO1 COG3883
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 307-406 1.63e-03

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 40.58  E-value: 1.63e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194440700 307 NNKDRLVTLEEFLRATEKKEFLepDSWETLDQQQLFTEDELKEYESIIAIQEN---ELKKRAEELQKQKEDLQRQHDHLE 383
Cdd:COG3883   97 RSGGSVSYLDVLLGSESFSDFL--DRLSALSKIADADADLLEELKADKAELEAkkaELEAKLAELEALKAELEAAKAELE 174

                         90       100
                 ....*....|....*....|....*
gi 194440700 384 AQKQEYHQAVQHL--EQKKLQQGIA 406
Cdd:COG3883  175 AQQAEQEALLAQLsaEEAAAEAQLA 199
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
250-338 3.37e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 37.46  E-value: 3.37e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194440700 250 FKLHDVNNDGFLDEQELEALFTRELEKVYNPQNAEDDMIEMEEERLRMREHVMSE------------IDNNKDRLVTLEE 317
Cdd:COG5126   11 FDLLDADGDGVLERDDFEALFRRLWATLFSEADTDGDGRISREEFVAGMESLFEAtvepfaraafdlLDTDGDGKISADE 90

                         90       100
                 ....*....|....*....|.
gi 194440700 318 FLRATEKKEFLEPDSWETLDQ 338
Cdd:COG5126   91 FRRLLTALGVSEEEADELFAR 111
PRK12704 PRK12704
phosphodiesterase; Provisional
 316-399 3.43e-03

phosphodiesterase; Provisional


Pssm-ID: 237177 [Multi-domain]  Cd Length: 520  Bit Score: 39.38  E-value: 3.43e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194440700 316 EEFLRATEK--KEFLEPDswETLDQQqlftEDELKEYESIIAIQENELKKRAEELQKQKEDLQRQHDHLEAQKQEYHQAV 393
Cdd:PRK12704  64 EEIHKLRNEfeKELRERR--NELQKL----EKRLLQKEENLDRKLELLEKREEELEKKEKELEQKQQELEKKEEELEELI 137


                 ....*.
gi 194440700 394 QHLEQK 399
Cdd:PRK12704 138 EEQLQE 143
MAD pfam05557
Mitotic checkpoint protein; This family consists of several eukaryotic mitotic checkpoint ...
 294-399 4.56e-03

Mitotic checkpoint protein; This family consists of several eukaryotic mitotic checkpoint (Mitotic arrest deficient or MAD) proteins. The mitotic spindle checkpoint monitors proper attachment of the bipolar spindle to the kinetochores of aligned sister chromatids and causes a cell cycle arrest in prometaphase when failures occur. Multiple components of the mitotic spindle checkpoint have been identified in yeast and higher eukaryotes. In S.cerevisiae, the existence of a Mad1-dependent complex containing Mad2, Mad3, Bub3 and Cdc20 has been demonstrated.


Pssm-ID: 461677 [Multi-domain]  Cd Length: 660  Bit Score: 39.34  E-value: 4.56e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194440700  294 RLRMREHVMSE-----IDNNKDRLVTLEEFLRATEKKEFLEPDSWETldqqQLFTEDELKEYESIIAIQENELKkraeEL 368
Cdd:pfam05557  59 LLEKREAEAEEalreqAELNRLKKKYLEALNKKLNEKESQLADAREV----ISCLKNELSELRRQIQRAELELQ----ST 130

                          90       100       110
                  ....*....|....*....|....*....|.
gi 194440700  369 QKQKEDLQRQHDHLEAQKQEYHQAVQHLEQK 399
Cdd:pfam05557 131 NSELEELQERLDLLKAKASEAEQLRQNLEKQ 161
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
247-332 6.64e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 36.69  E-value: 6.64e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194440700 247 KTFFKLHDVNNDGFLDEQELEALFTrelEKVYNPQNAeddmiemeeerlrmrEHVMSEIDNNKDRLVTLEEFLRATekKE 326
Cdd:COG5126   72 RAAFDLLDTDGDGKISADEFRRLLT---ALGVSEEEA---------------DELFARLDTDGDGKISFEEFVAAV--RD 131


                 ....*.
gi 194440700 327 FLEPDS 332
Cdd:COG5126  132 YYTPDA 137
CwlO1 COG3883
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 334-415 7.86e-03

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 38.27  E-value: 7.86e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194440700 334 ETLDQQQLFTEDELKEYESiiaiQENELKKRAEELQKQKEDLQRQHDHLEAQKQEYHQAVQHLEQKKLQQGIAPSGPAGE 413
Cdd:COG3883  143 AELEAKKAELEAKLAELEA----LKAELEAAKAELEAQQAEQEALLAQLSAEEAAAEAQLAELEAELAAAEAAAAAAAAA 218


                 ..
gi 194440700 414 LK 415
Cdd:COG3883  219 AA 220
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 250-321 8.36e-03

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 34.83  E-value: 8.36e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 194440700 250 FKLHDVNNDGFLDEQELEALFTRELEKVYNPQnaeddmiemeeerlrmREHVMSEIDNNKDRLVTLEEFLRA 321
Cdd:cd00051    6 FRLFDKDGDGTISADELKAALKSLGEGLSEEE----------------IDEMIREVDKDGDGKIDFEEFLEL 61
Smc COG1196
Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning]; ...
 262-403 9.39e-03

Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 440809 [Multi-domain]  Cd Length: 983  Bit Score: 38.38  E-value: 9.39e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194440700 262 DEQELEALFTRELEKVYNPQNAEDDMIEMEEERLRMREHVMSEIDNNKDRLVTLEEFLRATEKKEFLEPDSWETLdqqql 341
Cdd:COG1196  691 EELELEEALLAEEEEERELAEAEEERLEEELEEEALEEQLEAEREELLEELLEEEELLEEEALEELPEPPDLEEL----- 765

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 194440700 342 ftEDELKEYESII---------AIQEnelkkrAEELQKQKEDLQRQHDHLEAQKQEYHQAVQHLEQKKLQQ 403
Cdd:COG1196  766 --ERELERLEREIealgpvnllAIEE------YEELEERYDFLSEQREDLEEARETLEEAIEEIDRETRER 828
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH