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Conserved domains on  [gi|197304786|ref|NP_001127854|]
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IQ motif and SEC7 domain-containing protein 1 isoform a [Homo sapiens]

Protein Classification

IQ motif and SEC7 domain-containing protein( domain architecture ID 11116401)

IQ motif and SEC7 domain-containing protein (IQSEC) contains an IQ domain that may bind calmodulin, a PH domain that may mediate membrane localization by binding of phosphoinositides, and a SEC7 domain that functions as a guanine-nucleotide-exchange factor (GEF) for members of the ARF class of small GTPases

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Sec7 pfam01369
Sec7 domain; The Sec7 domain is a guanine-nucleotide-exchange-factor (GEF) for the pfam00025 ...
510-698 1.01e-93

Sec7 domain; The Sec7 domain is a guanine-nucleotide-exchange-factor (GEF) for the pfam00025 family.


:

Pssm-ID: 460178  Cd Length: 183  Bit Score: 296.68  E-value: 1.01e-93
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786   510 RKRHYRIGLNLFNKKPEKGVQYLIERGFVPDTPVGVAHFLLQRKGLSRQMIGEFLGNRqKQFNRDVLDCVVDEMDFSTME 589
Cdd:pfam01369    1 RKKLLREGIEKFNKKPKKGIEYLIEKGFIEDDPESIAKFLFETPGLDKKAIGEYLGKP-DEFNIEVLKAFVDLFDFKGLR 79
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786   590 LDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVvrqFRNPDTIFILAFAIILLNTDMYSPNVKpeRKMKLEDFI 669
Cdd:pfam01369   80 IDEALRLFLESFRLPGEAQKIDRIMEAFAERYYEQNPGV---FANADAAYVLAYSIIMLNTDLHNPNVK--KKMTLEDFI 154
                          170       180
                   ....*....|....*....|....*....
gi 197304786   670 KNLRGVDDGEDIPREMLMGIYERIRKREL 698
Cdd:pfam01369  155 RNLRGINDGKDFPDEYLEEIYDSIKKNEI 183
IQ_SEC7_PH pfam16453
PH domain; This PH domain is found in IQ motif and SEC7 domain-containing proteins.
730-859 5.82e-84

PH domain; This PH domain is found in IQ motif and SEC7 domain-containing proteins.


:

Pssm-ID: 465120  Cd Length: 127  Bit Score: 267.99  E-value: 5.82e-84
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786   730 CVLSLPHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDLLVVTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYPN 809
Cdd:pfam16453    1 PVLALPHRRLVCYCRLFEVPDPNKKQKLGLHQREVFLFNDLLVVTKIFSKKKSSVTYSFRQSFGLLGMQVSLFENSYYPH 80
                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|
gi 197304786   810 GIRLTSSVpgaDIKVLINFNAPNPQDRKKFTDDLRESIAEVQEMEKHRIE 859
Cdd:pfam16453   81 GIRLTSRV---DSKVLITFNARNEHDRKKFVEDLRESIAEVQEMEKLRIE 127
 
Name Accession Description Interval E-value
Sec7 pfam01369
Sec7 domain; The Sec7 domain is a guanine-nucleotide-exchange-factor (GEF) for the pfam00025 ...
510-698 1.01e-93

Sec7 domain; The Sec7 domain is a guanine-nucleotide-exchange-factor (GEF) for the pfam00025 family.


Pssm-ID: 460178  Cd Length: 183  Bit Score: 296.68  E-value: 1.01e-93
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786   510 RKRHYRIGLNLFNKKPEKGVQYLIERGFVPDTPVGVAHFLLQRKGLSRQMIGEFLGNRqKQFNRDVLDCVVDEMDFSTME 589
Cdd:pfam01369    1 RKKLLREGIEKFNKKPKKGIEYLIEKGFIEDDPESIAKFLFETPGLDKKAIGEYLGKP-DEFNIEVLKAFVDLFDFKGLR 79
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786   590 LDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVvrqFRNPDTIFILAFAIILLNTDMYSPNVKpeRKMKLEDFI 669
Cdd:pfam01369   80 IDEALRLFLESFRLPGEAQKIDRIMEAFAERYYEQNPGV---FANADAAYVLAYSIIMLNTDLHNPNVK--KKMTLEDFI 154
                          170       180
                   ....*....|....*....|....*....
gi 197304786   670 KNLRGVDDGEDIPREMLMGIYERIRKREL 698
Cdd:pfam01369  155 RNLRGINDGKDFPDEYLEEIYDSIKKNEI 183
Sec7 cd00171
Sec7 domain; Domain named after the S. cerevisiae SEC7 gene product. The Sec7 domain is the ...
510-698 1.43e-86

Sec7 domain; Domain named after the S. cerevisiae SEC7 gene product. The Sec7 domain is the central domain of the guanine-nucleotide-exchange factors (GEFs) of the ADP-ribosylation factor family of small GTPases (ARFs) . It carries the exchange factor activity.


Pssm-ID: 238100  Cd Length: 185  Bit Score: 277.18  E-value: 1.43e-86
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  510 RKRHYRIGLNLFNKKPEKGVQYLIERGFV-PDTPVGVAHFLLQRKGLSRQMIGEFLGNRQkQFNRDVLDCVVDEMDFSTM 588
Cdd:cd00171     1 RKTLLSEGRQLFNRKPKKGISFLIEKGFLeDDSPKEIAKFLYETEGLNKKAIGEYLGENN-EFNSLVLHEFVDLFDFSGL 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  589 ELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVVrqFRNPDTIFILAFAIILLNTDMYSPNVKpeRKMKLEDF 668
Cdd:cd00171    80 RLDEALRKFLQSFRLPGEAQKIDRLLEKFSERYCECNPGIF--SSSADAAYTLAYSIIMLNTDLHNPNVK--KKMTLEDF 155
                         170       180       190
                  ....*....|....*....|....*....|
gi 197304786  669 IKNLRGVDDGEDIPREMLMGIYERIRKREL 698
Cdd:cd00171   156 IKNLRGINDGEDFPREFLKELYDSIKNNEI 185
IQ_SEC7_PH pfam16453
PH domain; This PH domain is found in IQ motif and SEC7 domain-containing proteins.
730-859 5.82e-84

PH domain; This PH domain is found in IQ motif and SEC7 domain-containing proteins.


Pssm-ID: 465120  Cd Length: 127  Bit Score: 267.99  E-value: 5.82e-84
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786   730 CVLSLPHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDLLVVTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYPN 809
Cdd:pfam16453    1 PVLALPHRRLVCYCRLFEVPDPNKKQKLGLHQREVFLFNDLLVVTKIFSKKKSSVTYSFRQSFGLLGMQVSLFENSYYPH 80
                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|
gi 197304786   810 GIRLTSSVpgaDIKVLINFNAPNPQDRKKFTDDLRESIAEVQEMEKHRIE 859
Cdd:pfam16453   81 GIRLTSRV---DSKVLITFNARNEHDRKKFVEDLRESIAEVQEMEKLRIE 127
PH_IQSEC cd13318
IQ motif and SEC7 domain-containing protein family Pleckstrin homology domain; The IQSEC (also ...
735-865 2.88e-80

IQ motif and SEC7 domain-containing protein family Pleckstrin homology domain; The IQSEC (also called BRAG/Brefeldin A-resistant Arf-gunanine nucleotide exchange factor) family are a subset of Arf GEFs that have been shown to activate Arf6, which acts in the endocytic pathway to control the trafficking of a subset of cargo proteins including integrins and have key roles in the function and organization of distinct excitatory and inhibitory synapses in the retina. The family consists of 3 members: IQSEC1 (also called BRAG2/GEP100), IQSEC2 (also called BRAG1), and IQSEC3 (also called SynArfGEF, BRAG3, or KIAA1110). IQSEC1 interacts with clathrin and modulates cell adhesion by regulating integrin surface expression and in addition to Arf6, it also activates the class II Arfs, Arf4 and Arf5. Mutations in IQSEC2 cause non-syndromic X-linked intellectual disability as well as reduced activation of Arf substrates (Arf1, Arf6). IQSEC3 regulates Arf6 at inhibitory synapses and associates with the dystrophin-associated glycoprotein complex and S-SCAM. These members contains a IQ domain that may bind calmodulin, a PH domain that is thought to mediate membrane localization by binding of phosphoinositides, and a SEC7 domain that can promote GEF activity on ARF. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270128  Cd Length: 128  Bit Score: 257.63  E-value: 2.88e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  735 PHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDLLVVTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYPNGIRLT 814
Cdd:cd13318     1 PHRRLVCYCRLYEVPDPNKREKPGLHQREVFLFNDLLVVTKIFSKKKSSVTYSFRQSFSLLGMQVLLFETSHYPFGIRLT 80
                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 197304786  815 SSVpgaDIKVLINFNAPNPQDRKKFTDDLRESIAEVQEMEKHRIESELEKQ 865
Cdd:cd13318    81 SPL---DNKVLITFNARNESDRKKFVEDLRESILEVNEMESLRIEEELEKQ 128
Sec7 smart00222
Sec7 domain; Domain named after the S. cerevisiae SEC7 gene product, which is required for ...
507-698 8.35e-77

Sec7 domain; Domain named after the S. cerevisiae SEC7 gene product, which is required for proper protein transport through the Golgi. The domain facilitates guanine nucleotide exchange on the small GTPases, ARFs (ADP ribosylation factors).


Pssm-ID: 214569 [Multi-domain]  Cd Length: 189  Bit Score: 250.67  E-value: 8.35e-77
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786    507 DVIRKRHYRIGLNLFNKKPEKGVQYLIERGFVP-DTPVGVAHFLLQRKGLSRQMIGEFLGNRqKQFNRDVLDCVVDEMDF 585
Cdd:smart00222    1 SKGRKKLLSEGIVKFNDKPKKGIQSLQEKGFLAnEDPQDVADFLSKNEGLNKKAIGDYLGEH-DEFNRLVLHAFVDLFDF 79
                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786    586 STMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVVRqFRNPDTIFILAFAIILLNTDMYSPNVKpeRKMKL 665
Cdd:smart00222   80 SAKDLDQALREFLESFRLPGEAQKIDRLLEAFSSRYCECNPGVFS-KANADAAYTLAYSLIMLNTDLHNPNVK--KKMTL 156
                           170       180       190
                    ....*....|....*....|....*....|...
gi 197304786    666 EDFIKNLRGVDDGEDIPREMLMGIYERIRKREL 698
Cdd:smart00222  157 EDFIKNVRGSNDGEDLPREFLEELYDSIKNNEI 189
PLN03076 PLN03076
ARF guanine nucleotide exchange factor (ARF-GEF); Provisional
510-710 4.49e-52

ARF guanine nucleotide exchange factor (ARF-GEF); Provisional


Pssm-ID: 215560 [Multi-domain]  Cd Length: 1780  Bit Score: 200.82  E-value: 4.49e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  510 RKRHYRI----GLNLFNKKPEKGVQYLIERGFVPDTPVGVAHFLLQRKGLSRQMIGEFLGNRQkQFNRDVLDCVVDEMDF 585
Cdd:PLN03076  612 QRRAYKLelqeGISLFNRKPKKGIEFLINANKVGESPEEIAAFLKDASGLNKTLIGDYLGERE-DLSLKVMHAYVDSFDF 690
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  586 STMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVvrqFRNPDTIFILAFAIILLNTDMYSPNVKpeRKMKL 665
Cdd:PLN03076  691 QGMEFDEAIRAFLQGFRLPGEAQKIDRIMEKFAERYCKCNPKA---FSSADTAYVLAYSVIMLNTDAHNPMVK--NKMSA 765
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 197304786  666 EDFIKNLRGVDDGEDIPREMLMGIYERIRKRELKTNEDHVSQVQK 710
Cdd:PLN03076  766 DDFIRNNRGIDDGKDLPEEFMRSLYERISKNEIKMKEDDLVPQQK 810
PH smart00233
Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The ...
753-848 2.95e-05

Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. PH domains have been found to possess inserted domains (such as in PLC gamma, syntrophins) and to be inserted within other domains. Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. Point mutations cluster into the positively charged end of the molecule around the predicted binding site for phosphatidylinositol lipids.


Pssm-ID: 214574 [Multi-domain]  Cd Length: 102  Bit Score: 44.08  E-value: 2.95e-05
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786    753 KPQKLGLHQREIFLFNDLLVvtkIFQKKKNSVTYSFRQSFSLYGMQVLLFENQY---YPNGIRLTSSvpgaDIKVLInFN 829
Cdd:smart00233   12 GGGKKSWKKRYFVLFNSTLL---YYKSKKDKKSYKPKGSIDLSGCTVREAPDPDsskKPHCFEIKTS----DRKTLL-LQ 83
                            90
                    ....*....|....*....
gi 197304786    830 APNPQDRKKFTDDLRESIA 848
Cdd:smart00233   84 AESEEEREKWVEALRKAIA 102
 
Name Accession Description Interval E-value
Sec7 pfam01369
Sec7 domain; The Sec7 domain is a guanine-nucleotide-exchange-factor (GEF) for the pfam00025 ...
510-698 1.01e-93

Sec7 domain; The Sec7 domain is a guanine-nucleotide-exchange-factor (GEF) for the pfam00025 family.


Pssm-ID: 460178  Cd Length: 183  Bit Score: 296.68  E-value: 1.01e-93
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786   510 RKRHYRIGLNLFNKKPEKGVQYLIERGFVPDTPVGVAHFLLQRKGLSRQMIGEFLGNRqKQFNRDVLDCVVDEMDFSTME 589
Cdd:pfam01369    1 RKKLLREGIEKFNKKPKKGIEYLIEKGFIEDDPESIAKFLFETPGLDKKAIGEYLGKP-DEFNIEVLKAFVDLFDFKGLR 79
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786   590 LDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVvrqFRNPDTIFILAFAIILLNTDMYSPNVKpeRKMKLEDFI 669
Cdd:pfam01369   80 IDEALRLFLESFRLPGEAQKIDRIMEAFAERYYEQNPGV---FANADAAYVLAYSIIMLNTDLHNPNVK--KKMTLEDFI 154
                          170       180
                   ....*....|....*....|....*....
gi 197304786   670 KNLRGVDDGEDIPREMLMGIYERIRKREL 698
Cdd:pfam01369  155 RNLRGINDGKDFPDEYLEEIYDSIKKNEI 183
Sec7 cd00171
Sec7 domain; Domain named after the S. cerevisiae SEC7 gene product. The Sec7 domain is the ...
510-698 1.43e-86

Sec7 domain; Domain named after the S. cerevisiae SEC7 gene product. The Sec7 domain is the central domain of the guanine-nucleotide-exchange factors (GEFs) of the ADP-ribosylation factor family of small GTPases (ARFs) . It carries the exchange factor activity.


Pssm-ID: 238100  Cd Length: 185  Bit Score: 277.18  E-value: 1.43e-86
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  510 RKRHYRIGLNLFNKKPEKGVQYLIERGFV-PDTPVGVAHFLLQRKGLSRQMIGEFLGNRQkQFNRDVLDCVVDEMDFSTM 588
Cdd:cd00171     1 RKTLLSEGRQLFNRKPKKGISFLIEKGFLeDDSPKEIAKFLYETEGLNKKAIGEYLGENN-EFNSLVLHEFVDLFDFSGL 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  589 ELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVVrqFRNPDTIFILAFAIILLNTDMYSPNVKpeRKMKLEDF 668
Cdd:cd00171    80 RLDEALRKFLQSFRLPGEAQKIDRLLEKFSERYCECNPGIF--SSSADAAYTLAYSIIMLNTDLHNPNVK--KKMTLEDF 155
                         170       180       190
                  ....*....|....*....|....*....|
gi 197304786  669 IKNLRGVDDGEDIPREMLMGIYERIRKREL 698
Cdd:cd00171   156 IKNLRGINDGEDFPREFLKELYDSIKNNEI 185
IQ_SEC7_PH pfam16453
PH domain; This PH domain is found in IQ motif and SEC7 domain-containing proteins.
730-859 5.82e-84

PH domain; This PH domain is found in IQ motif and SEC7 domain-containing proteins.


Pssm-ID: 465120  Cd Length: 127  Bit Score: 267.99  E-value: 5.82e-84
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786   730 CVLSLPHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDLLVVTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYPN 809
Cdd:pfam16453    1 PVLALPHRRLVCYCRLFEVPDPNKKQKLGLHQREVFLFNDLLVVTKIFSKKKSSVTYSFRQSFGLLGMQVSLFENSYYPH 80
                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|
gi 197304786   810 GIRLTSSVpgaDIKVLINFNAPNPQDRKKFTDDLRESIAEVQEMEKHRIE 859
Cdd:pfam16453   81 GIRLTSRV---DSKVLITFNARNEHDRKKFVEDLRESIAEVQEMEKLRIE 127
PH_IQSEC cd13318
IQ motif and SEC7 domain-containing protein family Pleckstrin homology domain; The IQSEC (also ...
735-865 2.88e-80

IQ motif and SEC7 domain-containing protein family Pleckstrin homology domain; The IQSEC (also called BRAG/Brefeldin A-resistant Arf-gunanine nucleotide exchange factor) family are a subset of Arf GEFs that have been shown to activate Arf6, which acts in the endocytic pathway to control the trafficking of a subset of cargo proteins including integrins and have key roles in the function and organization of distinct excitatory and inhibitory synapses in the retina. The family consists of 3 members: IQSEC1 (also called BRAG2/GEP100), IQSEC2 (also called BRAG1), and IQSEC3 (also called SynArfGEF, BRAG3, or KIAA1110). IQSEC1 interacts with clathrin and modulates cell adhesion by regulating integrin surface expression and in addition to Arf6, it also activates the class II Arfs, Arf4 and Arf5. Mutations in IQSEC2 cause non-syndromic X-linked intellectual disability as well as reduced activation of Arf substrates (Arf1, Arf6). IQSEC3 regulates Arf6 at inhibitory synapses and associates with the dystrophin-associated glycoprotein complex and S-SCAM. These members contains a IQ domain that may bind calmodulin, a PH domain that is thought to mediate membrane localization by binding of phosphoinositides, and a SEC7 domain that can promote GEF activity on ARF. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270128  Cd Length: 128  Bit Score: 257.63  E-value: 2.88e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  735 PHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDLLVVTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYPNGIRLT 814
Cdd:cd13318     1 PHRRLVCYCRLYEVPDPNKREKPGLHQREVFLFNDLLVVTKIFSKKKSSVTYSFRQSFSLLGMQVLLFETSHYPFGIRLT 80
                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 197304786  815 SSVpgaDIKVLINFNAPNPQDRKKFTDDLRESIAEVQEMEKHRIESELEKQ 865
Cdd:cd13318    81 SPL---DNKVLITFNARNESDRKKFVEDLRESILEVNEMESLRIEEELEKQ 128
Sec7 smart00222
Sec7 domain; Domain named after the S. cerevisiae SEC7 gene product, which is required for ...
507-698 8.35e-77

Sec7 domain; Domain named after the S. cerevisiae SEC7 gene product, which is required for proper protein transport through the Golgi. The domain facilitates guanine nucleotide exchange on the small GTPases, ARFs (ADP ribosylation factors).


Pssm-ID: 214569 [Multi-domain]  Cd Length: 189  Bit Score: 250.67  E-value: 8.35e-77
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786    507 DVIRKRHYRIGLNLFNKKPEKGVQYLIERGFVP-DTPVGVAHFLLQRKGLSRQMIGEFLGNRqKQFNRDVLDCVVDEMDF 585
Cdd:smart00222    1 SKGRKKLLSEGIVKFNDKPKKGIQSLQEKGFLAnEDPQDVADFLSKNEGLNKKAIGDYLGEH-DEFNRLVLHAFVDLFDF 79
                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786    586 STMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVVRqFRNPDTIFILAFAIILLNTDMYSPNVKpeRKMKL 665
Cdd:smart00222   80 SAKDLDQALREFLESFRLPGEAQKIDRLLEAFSSRYCECNPGVFS-KANADAAYTLAYSLIMLNTDLHNPNVK--KKMTL 156
                           170       180       190
                    ....*....|....*....|....*....|...
gi 197304786    666 EDFIKNLRGVDDGEDIPREMLMGIYERIRKREL 698
Cdd:smart00222  157 EDFIKNVRGSNDGEDLPREFLEELYDSIKNNEI 189
PLN03076 PLN03076
ARF guanine nucleotide exchange factor (ARF-GEF); Provisional
510-710 4.49e-52

ARF guanine nucleotide exchange factor (ARF-GEF); Provisional


Pssm-ID: 215560 [Multi-domain]  Cd Length: 1780  Bit Score: 200.82  E-value: 4.49e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  510 RKRHYRI----GLNLFNKKPEKGVQYLIERGFVPDTPVGVAHFLLQRKGLSRQMIGEFLGNRQkQFNRDVLDCVVDEMDF 585
Cdd:PLN03076  612 QRRAYKLelqeGISLFNRKPKKGIEFLINANKVGESPEEIAAFLKDASGLNKTLIGDYLGERE-DLSLKVMHAYVDSFDF 690
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  586 STMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVvrqFRNPDTIFILAFAIILLNTDMYSPNVKpeRKMKL 665
Cdd:PLN03076  691 QGMEFDEAIRAFLQGFRLPGEAQKIDRIMEKFAERYCKCNPKA---FSSADTAYVLAYSVIMLNTDAHNPMVK--NKMSA 765
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 197304786  666 EDFIKNLRGVDDGEDIPREMLMGIYERIRKRELKTNEDHVSQVQK 710
Cdd:PLN03076  766 DDFIRNNRGIDDGKDLPEEFMRSLYERISKNEIKMKEDDLVPQQK 810
PH smart00233
Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The ...
753-848 2.95e-05

Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. PH domains have been found to possess inserted domains (such as in PLC gamma, syntrophins) and to be inserted within other domains. Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. Point mutations cluster into the positively charged end of the molecule around the predicted binding site for phosphatidylinositol lipids.


Pssm-ID: 214574 [Multi-domain]  Cd Length: 102  Bit Score: 44.08  E-value: 2.95e-05
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786    753 KPQKLGLHQREIFLFNDLLVvtkIFQKKKNSVTYSFRQSFSLYGMQVLLFENQY---YPNGIRLTSSvpgaDIKVLInFN 829
Cdd:smart00233   12 GGGKKSWKKRYFVLFNSTLL---YYKSKKDKKSYKPKGSIDLSGCTVREAPDPDsskKPHCFEIKTS----DRKTLL-LQ 83
                            90
                    ....*....|....*....
gi 197304786    830 APNPQDRKKFTDDLRESIA 848
Cdd:smart00233   84 AESEEEREKWVEALRKAIA 102
PH1_FGD5_FGD6 cd13389
FYVE, RhoGEF and PH domain containing/faciogenital dysplasia proteins 5 and 6, N-terminal ...
759-849 2.65e-04

FYVE, RhoGEF and PH domain containing/faciogenital dysplasia proteins 5 and 6, N-terminal Pleckstrin Homology (PH) domain; FGD5 regulates promotes angiogenesis of vascular endothelial growth factor (VEGF) in vascular endothelial cells, including network formation, permeability, directional movement, and proliferation. The specific function of FGD6 is unknown. In general, FGDs have a RhoGEF (DH) domain, followed by a PH domain, a FYVE domain and a C-terminal PH domain. All FGDs are guanine nucleotide exchange factors that activate the Rho GTPase Cdc42, an important regulator of membrane trafficking. The RhoGEF domain is responsible for GEF catalytic activity, while the PH domain is involved in intracellular targeting of the DH domain. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275424  Cd Length: 124  Bit Score: 41.87  E-value: 2.65e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 197304786  759 LHQREIFLFNDLLVVTKIFQkkkNSVTYSFRQSFSLYGMQVLLFENQYYPNGIRLTSSVPGADIKvlinfnAPNPQDRKK 838
Cdd:cd13389    28 MQPRYFFLFNDCLLYTTPVQ---SSGMLKLNNELPLSGMKVKLPEDEEYSNEFQIISTKRSFTLI------ASSEEERDE 98
                          90
                  ....*....|.
gi 197304786  839 FTDDLRESIAE 849
Cdd:cd13389    99 WVKALSRAIEE 109
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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