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Conserved domains on  [gi|300244501|ref|NP_001177900|]
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piwi-like protein 1 isoform 2 [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Piwi_piwi-like_Euk cd04658
Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found ...
 401-823 0e+00

Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related gene-silencing mechanisms mediated by short RNA molecules, including siRNAs, miRNAs, and heterochromatin-related guide RNAs. The mechanism in Piwi is believed to be similar to that in Argonaute, the central component of the RNA-induced silencing complex (RISC). The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the 5' anchoring of the guide RNA and the other, the catalytic site for slicing.


:

Pssm-ID: 240016 [Multi-domain]  Cd Length: 448  Bit Score: 624.29  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 401 NVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKtFDYNPQFADWSK 480
Cdd:cd04658    1 NLMKELAEHTKLNPKERYDTIRQFIQRIQKNPSVQELLKKWGIELDSNPLKIQGRVLPPEQIIMGNV-FVYANSNADWKR 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 481 ETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEV-DDRTEAYLRVLQQKVTADTQIVVCL 559
Cdd:cd04658   80 EIRNQPLYDAVNLNNWVLIYPSRDQREAESFLQTLKQVAGPMGIQISPPKIIKVkDDRIETYIRALKDAFRSDPQLVVII 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 560 LSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIP---LKLVMIVGIDCYHD 636
Cdd:cd04658  160 LPGNKKDLYDAIKKFCCVECPVPSQVITSRTLKKKKNLRSIASKIALQINAKLGGIPWTVEIPpfiLKNTMIVGIDVYHD 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 637 MTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQEL-VDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNY 715
Cdd:cd04658  240 TITKKKSVVGFVASLNKSITKWFSKYISQVRGQEEiIDSLGKSMKKALKAYKKENKKLPSRIIIYRDGVGDGQLKKVKEY 319

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 716 EVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYN 795
Cdd:cd04658  320 EVPQIKKAIKQYSENYSPKLAYIVVNKRINTRFFNQGGNNFSNPPPGTVVDSEITKPEWYDFFLVSQSVRQGTVTPTHYN 399

                        410       420
                 ....*....|....*....|....*...
gi 300244501 796 VIYDNSGLKPDHIQRLTYKLCHIYYNWP 823
Cdd:cd04658  400 VLYDTTGLKPDHLQRLTYKLCHLYYNWS 427
PAZ smart00949
This domain is named PAZ after the proteins Piwi Argonaut and Zwille; This domain is found in ...
 277-415 1.88e-64

This domain is named PAZ after the proteins Piwi Argonaut and Zwille; This domain is found in two families of proteins that are involved in post-transcriptional gene silencing. These are the Piwi family and the Dicer family, that includes the Carpel factory protein. The function of the domains is unknown but has been suggested to mediate complex formation between proteins of the Piwi and Dicer families by hetero-dimerisation. The three-dimensional structure of this domain has been solved. The PAZ domain is composed of two subdomains. One subdomain is similar to the OB fold, albeit with a different topology. The OB-fold is well known as a single-stranded nucleic acid binding fold. The second subdomain is composed of a beta-hairpin followed by an alpha-helix. The PAZ domains shows low-affinity nucleic acid binding and appears to interact with the 3' ends of single-stranded regions of RNA in the cleft between the two subdomains. PAZ can bind the characteristic two-base 3' overhangs of siRNAs, indicating that although PAZ may not be a primary nucleic acid binding site in Dicer or RISC, it may contribute to the specific and productive incorporation of siRNAs and miRNAs into the RNAi pathway.


:

Pssm-ID: 198017  Cd Length: 138  Bit Score: 211.76  E-value: 1.88e-64
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   277 ETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITD 356
Cdd:smart00949   1 ETVLDFMRQLPSQGNRSNFQDRCAKDLKGLIVLTRYNNKTYRIDDIDWNLAPKSTFEKSDGSEITFVEYYKQKYNITIRD 80

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 300244501   357 LKQPVLVSQPKRRRGPGGTlPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPE 415
Cdd:smart00949  81 PNQPLLVSRPKRRRNQNGK-GEPVLLPPELCFITGLTDRMRKDFMLMKSIADRTRLSPL 138
GAGE pfam05831
GAGE protein; This family consists of several GAGE and XAGE proteins which are found ...
 16-106 2.47e-32

GAGE protein; This family consists of several GAGE and XAGE proteins which are found exclusively in humans. The function of this family is unknown although they have been implicated in human cancers.


:

Pssm-ID: 461753  Cd Length: 107  Bit Score: 120.95  E-value: 2.47e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   16 QETAQLVGSTASQQPGYIQPRPQPPPAEGELFGRGRQRGTaGGTAKSQGLQISAGFQELSLAERGGRRRDFHDLGVNTRQ 95
Cdd:pfam05831  18 QESSQLVGPVVAQQPSDEQPQQEEPPTESQDITPGQERED-EGASAIQGPELEADLQELALEKTGGERGDGPDVKGKTLP 96

                          90
                  ....*....|.
gi 300244501   96 NLDHVKESKTG 106
Cdd:pfam05831  97 NLEPVKMPEAG 107
 
Name Accession Description Interval E-value
Piwi_piwi-like_Euk cd04658
Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found ...
 401-823 0e+00

Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related gene-silencing mechanisms mediated by short RNA molecules, including siRNAs, miRNAs, and heterochromatin-related guide RNAs. The mechanism in Piwi is believed to be similar to that in Argonaute, the central component of the RNA-induced silencing complex (RISC). The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the 5' anchoring of the guide RNA and the other, the catalytic site for slicing.


Pssm-ID: 240016 [Multi-domain]  Cd Length: 448  Bit Score: 624.29  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 401 NVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKtFDYNPQFADWSK 480
Cdd:cd04658    1 NLMKELAEHTKLNPKERYDTIRQFIQRIQKNPSVQELLKKWGIELDSNPLKIQGRVLPPEQIIMGNV-FVYANSNADWKR 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 481 ETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEV-DDRTEAYLRVLQQKVTADTQIVVCL 559
Cdd:cd04658   80 EIRNQPLYDAVNLNNWVLIYPSRDQREAESFLQTLKQVAGPMGIQISPPKIIKVkDDRIETYIRALKDAFRSDPQLVVII 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 560 LSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIP---LKLVMIVGIDCYHD 636
Cdd:cd04658  160 LPGNKKDLYDAIKKFCCVECPVPSQVITSRTLKKKKNLRSIASKIALQINAKLGGIPWTVEIPpfiLKNTMIVGIDVYHD 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 637 MTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQEL-VDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNY 715
Cdd:cd04658  240 TITKKKSVVGFVASLNKSITKWFSKYISQVRGQEEiIDSLGKSMKKALKAYKKENKKLPSRIIIYRDGVGDGQLKKVKEY 319

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 716 EVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYN 795
Cdd:cd04658  320 EVPQIKKAIKQYSENYSPKLAYIVVNKRINTRFFNQGGNNFSNPPPGTVVDSEITKPEWYDFFLVSQSVRQGTVTPTHYN 399

                        410       420
                 ....*....|....*....|....*...
gi 300244501 796 VIYDNSGLKPDHIQRLTYKLCHIYYNWP 823
Cdd:cd04658  400 VLYDTTGLKPDHLQRLTYKLCHLYYNWS 427
Piwi pfam02171
Piwi domain; This domain is found in the protein Piwi and its relatives. The function of this ...
 555-827 6.19e-117

Piwi domain; This domain is found in the protein Piwi and its relatives. The function of this domain is the dsRNA guided hydrolysis of ssRNA. Determination of the crystal structure of Argonaute reveals that PIWI is an RNase H domain, and identifies Argonaute as Slicer, the enzyme that cleaves mRNA in the RNAi RISC complex. In addition, Mg+2 dependence and production of 3'-OH and 5' phosphate products are shared characteriztics of RNaseH and RISC. The PIWI domain core has a tertiary structure belonging to the RNase H family of enzymes. RNase H fold proteins all have a five-stranded mixed beta-sheet surrounded by helices. By analogy to RNase H enzymes which cleave single-stranded RNA guided by the DNA strand in an RNA/DNA hybrid, the PIWI domain can be inferred to cleave single-stranded RNA, for example mRNA, guided by double stranded siRNA.


Pssm-ID: 396649  Cd Length: 296  Bit Score: 356.26  E-value: 6.19e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501  555 IVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTvMAIATKIALQMNCKMGGE-LWRVDIPLKLVMIVGIDC 633
Cdd:pfam02171   1 LILVILPEKNKDLYHSIKKYLETDLGIPSQCILSKTILKRTL-KQTLTNVLLKINVKLGGInYWIVEIKPKVDVIIGFDI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501  634 YHDM--TAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKT 711
Cdd:pfam02171  80 SHGTagTDDNPSVAAVVASFDKGNSRYFGTVRTQASGQELLEPLKDIIKELLRSFQKSSRKKPERIIVYRDGVSEGQFPQ 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501  712 LVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSG-GRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVS 790
Cdd:pfam02171 160 VLNYEVNQIKEACKSLGPGYNPKLTVIVVQKRHHTRFFANDKpDGDQNPPPGTVVDDVITLPEYYDFYLCSHAGLQGTVK 239

                         250       260       270
                  ....*....|....*....|....*....|....*..
gi 300244501  791 PTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPVSAS 827
Cdd:pfam02171 240 PTHYTVLYDEIGLSADELQNLTYKLCHMYYRSTRPIS 276
Piwi smart00950
This domain is found in the protein Piwi and its relatives; The function of this domain is the ...
 555-827 7.89e-111

This domain is found in the protein Piwi and its relatives; The function of this domain is the dsRNA guided hydrolysis of ssRNA. Determination of the crystal structure of Argonaute reveals that PIWI is an RNase H domain, and identifies Argonaute as Slicer, the enzyme that cleaves mRNA in the RNAi RISC complex.. In addition, Mg+2 dependence and production of 3'-OH and 5' phosphate products are shared characteristics of RNaseH and RISC. The PIWI domain core has a tertiary structure belonging to the RNase H family of enzymes. RNase H fold proteins all have a five-stranded mixed beta-sheet surrounded by helices. By analogy to RNase H enzymes which cleave single-stranded RNA guided by the DNA strand in an RNA/DNA hybrid, the PIWI domain can be inferred to cleave single-stranded RNA, for example mRNA, guided by double stranded siRNA.


Pssm-ID: 214930  Cd Length: 301  Bit Score: 340.85  E-value: 7.89e-111
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   555 IVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTL---GKQQTVMAIATKIALQMNCKMGGELWRVD---IPLKLVMI 628
Cdd:smart00950   2 IVVILPGEKKTDLYHEIKKYLETKLGVPTQCVQAKTLdkvSKRRKLKQYLTNVALKINAKLGGINWVLDvppIPLKPTLI 81

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   629 VGIDCYHDMTAGRRSIAGFVASINeGMTRWFSRCIFQD-RGQELVDGLKVCLQAALRAW-NSCNEYMPSRIIVYRDGVGD 706
Cdd:smart00950  82 IGIDVSHPSAGKGGSVAPSVAAFV-ASGNYLSGNFYQAfVREQGSRQLKEILREALKKYyKSNRKRLPDRIVVYRDGVSE 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   707 GQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRS 786
Cdd:smart00950 161 GQFKQVLEYEVKAIKKACKELGPDYKPKLTVIVVQKRHHTRFFPEDGNGRVNVPPGTVVDSVITSPEWYDFYLVSHAGLQ 240

                          250       260       270       280
                   ....*....|....*....|....*....|....*....|.
gi 300244501   787 GSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPVSAS 827
Cdd:smart00950 241 GTARPTHYTVLYDEGNLDPDELQRLTYKLCHLYYRSTRPVS 281
PAZ smart00949
This domain is named PAZ after the proteins Piwi Argonaut and Zwille; This domain is found in ...
 277-415 1.88e-64

This domain is named PAZ after the proteins Piwi Argonaut and Zwille; This domain is found in two families of proteins that are involved in post-transcriptional gene silencing. These are the Piwi family and the Dicer family, that includes the Carpel factory protein. The function of the domains is unknown but has been suggested to mediate complex formation between proteins of the Piwi and Dicer families by hetero-dimerisation. The three-dimensional structure of this domain has been solved. The PAZ domain is composed of two subdomains. One subdomain is similar to the OB fold, albeit with a different topology. The OB-fold is well known as a single-stranded nucleic acid binding fold. The second subdomain is composed of a beta-hairpin followed by an alpha-helix. The PAZ domains shows low-affinity nucleic acid binding and appears to interact with the 3' ends of single-stranded regions of RNA in the cleft between the two subdomains. PAZ can bind the characteristic two-base 3' overhangs of siRNAs, indicating that although PAZ may not be a primary nucleic acid binding site in Dicer or RISC, it may contribute to the specific and productive incorporation of siRNAs and miRNAs into the RNAi pathway.


Pssm-ID: 198017  Cd Length: 138  Bit Score: 211.76  E-value: 1.88e-64
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   277 ETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITD 356
Cdd:smart00949   1 ETVLDFMRQLPSQGNRSNFQDRCAKDLKGLIVLTRYNNKTYRIDDIDWNLAPKSTFEKSDGSEITFVEYYKQKYNITIRD 80

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 300244501   357 LKQPVLVSQPKRRRGPGGTlPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPE 415
Cdd:smart00949  81 PNQPLLVSRPKRRRNQNGK-GEPVLLPPELCFITGLTDRMRKDFMLMKSIADRTRLSPL 138
PAZ_piwi_like cd02845
PAZ domain, Piwi_like subfamily. In multi-cellular organisms, the Piwi protein appears to be ...
 276-393 1.73e-62

PAZ domain, Piwi_like subfamily. In multi-cellular organisms, the Piwi protein appears to be essential for the maintenance of germline stem cells. In the Drosophila male germline, Piwi was shown to be involved in the silencing of retrotransposons in the male gametes. The Piwi proteins share their domain architecture with other members of the argonaute family. The PAZ domain has been named after the proteins Piwi, Argonaut, and Zwille. PAZ is found in two families of proteins that are essential components of RNA-mediated gene-silencing pathways, including RNA interference, the Piwi and Dicer families. PAZ functions as a nucleic acid binding domain, with a strong preference for single-stranded nucleic acids (RNA or DNA) or RNA duplexes with single-stranded 3' overhangs. It has been suggested that the PAZ domain provides a unique mode for the recognition of the two 3'-terminal nucleotides in single-stranded nucleic acids and buries the 3' OH group, and that it might recognize characteristic 3' overhangs in siRNAs within RISC (RNA-induced silencing) and other complexes.


Pssm-ID: 239211  Cd Length: 117  Bit Score: 205.57  E-value: 1.73e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 276 SETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEIT 355
Cdd:cd02845    1 STTVLDRMHKLYRQETDERFREECEKELIGSIVLTRYNNKTYRIDDIDFDKTPLSTFKKSDGTEITFVEYYKKQYNIEIT 80

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 300244501 356 DLKQPVLVSQPKRRRGPGGTlPGPAMLIPELCYLTGLT 393
Cdd:cd02845   81 DLNQPLLVSRPKRRDPRGGE-KEPIYLIPELCFLTGLT 117
PAZ pfam02170
PAZ domain; This domain is named PAZ after the proteins Piwi Argonaut and Zwille. This domain ...
 280-413 1.83e-44

PAZ domain; This domain is named PAZ after the proteins Piwi Argonaut and Zwille. This domain is found in two families of proteins that are involved in post-transcriptional gene silencing. These are the Piwi family and the Dicer family, that includes the Carpel factory protein. The function of the domains is unknown but has been suggested to mediate complex formation between proteins of the Piwi and Dicer families by hetero-dimerization. The three-dimensional structure of this domain has been solved. The PAZ domain is composed of two subdomains. One subdomain is similar to the OB fold, albeit with a different topology. The OB-fold is well known as a single-stranded nucleic acid binding fold. The second subdomain is composed of a beta-hairpin followed by an alpha-helix. The PAZ domains shows low-affinity nucleic acid binding and appears to interact with the 3' ends of single-stranded regions of RNA in the cleft between the two subdomains. PAZ can bind the characteriztic two-base 3' overhangs of siRNAs, indicating that although PAZ may not be a primary nucleic acid binding site in Dicer or RISC, it may contribute to the specific and productive incorporation of siRNAs and miRNAs into the RNAi pathway.


Pssm-ID: 460472  Cd Length: 123  Bit Score: 156.20  E-value: 1.83e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501  280 LDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNN-KTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLK 358
Cdd:pfam02170   1 LDFLKRLQQQKDRRDFRKEAKKALKGLKVYTTYNNpRTYRIDGITFDPTPESTFPLKDGKEITVVDYFKKKYNIDLKYPD 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 300244501  359 QPVLVSQPKRRRgpggtlpgpAMLIPELCYltgLTDKMRNDFNVMKDLAVHTRLT 413
Cdd:pfam02170  81 QPLLLVGKKRPK---------VYLPPELCN---LVDGQRYTKKLMPSIAQRTRLL 123
PLN03202 PLN03202
protein argonaute; Provisional
 251-819 3.11e-39

protein argonaute; Provisional


Pssm-ID: 215631 [Multi-domain]  Cd Length: 900  Bit Score: 156.80  E-value: 3.11e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 251 GFTTSILQYENSIMLCTDVSHK-VLRSETVLDFMFNfyHQTEEHKFQ---EQVSKELIGLVVLTKYNNKTYRVddIDWDQ 326
Cdd:PLN03202 241 GFHSSFRTTQGGLSLNIDVSTTmIVQPGPVVDFLIA--NQNVRDPFQidwSKAKRMLKNLRVKVSPSNQEYKI--TGLSE 316

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 327 NP--KSTF--KKADGS-------EVSFLEYYRKQYNQEIT---DLkqPVL-VSQPKRrrgpggtlpgPAMLIPELCYLTG 391
Cdd:PLN03202 317 KPckEQTFslKQRNGNgnevetvEITVYDYFVKHRGIELRysgDL--PCInVGKPKR----------PTYFPIELCSLVS 384

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 392 LTdkmrndfNVMKDLAVHTR--LTPEQRQR--EVGRLIDYIHKNDNVQRE--LRDWGLSFDSNLLSFSGRILQTEKIhQG 465
Cdd:PLN03202 385 LQ-------RYTKALSTLQRssLVEKSRQKpqERMKVLTDALKSSNYDADpmLRSCGISISSQFTQVEGRVLPAPKL-KV 456

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 466 GKTFDYNPQFADWSKETRgaPLISVKPLDNWLLIytrrNYEA---ANSLIQNLFKVTPAMGM-------------QMRKA 529
Cdd:PLN03202 457 GNGEDFFPRNGRWNFNNK--KLVEPTKIERWAVV----NFSArcdIRHLVRDLIKCGEMKGInieppfdvfeenpQFRRA 530

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 530 -IMIEVDDRTEAylrvLQQKVTADTQIVVCLLSsNRK--DKYDAIKKYLCTDCPTPSQCVVARTLGKQQTvmaiaTKIAL 606
Cdd:PLN03202 531 pPPVRVEKMFEQ----IQSKLPGPPQFLLCILP-ERKnsDIYGPWKKKNLSEFGIVTQCIAPTRVNDQYL-----TNVLL 600

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 607 QMNCKMGG--ELWRVD----IPL--KL-VMIVGIDCYHDmTAGRR---SIAGFVASINEGMTRWFSRCI-FQDRGQELVD 673
Cdd:PLN03202 601 KINAKLGGlnSLLAIEhspsIPLvsKVpTIILGMDVSHG-SPGQSdvpSIAAVVSSRQWPLISRYRASVrTQSPKVEMID 679

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 674 GL----------KVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKR 743
Cdd:PLN03202 680 SLfkpvgdkdddGIIRELLLDFYTSSGKRKPEQIIIFRDGVSESQFNQVLNIELDQIIEACKFLDESWSPKFTVIVAQKN 759

                        570       580       590       600       610       620       630
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 300244501 744 VNTRFFaQSGGRlQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIY 819
Cdd:PLN03202 760 HHTKFF-QAGSP-DNVPPGTVVDNKICHPRNNDFYMCAHAGMIGTTRPTHYHVLLDEIGFSADDLQELVHSLSYVY 833
GAGE pfam05831
GAGE protein; This family consists of several GAGE and XAGE proteins which are found ...
 16-106 2.47e-32

GAGE protein; This family consists of several GAGE and XAGE proteins which are found exclusively in humans. The function of this family is unknown although they have been implicated in human cancers.


Pssm-ID: 461753  Cd Length: 107  Bit Score: 120.95  E-value: 2.47e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   16 QETAQLVGSTASQQPGYIQPRPQPPPAEGELFGRGRQRGTaGGTAKSQGLQISAGFQELSLAERGGRRRDFHDLGVNTRQ 95
Cdd:pfam05831  18 QESSQLVGPVVAQQPSDEQPQQEEPPTESQDITPGQERED-EGASAIQGPELEADLQELALEKTGGERGDGPDVKGKTLP 96

                          90
                  ....*....|.
gi 300244501   96 NLDHVKESKTG 106
Cdd:pfam05831  97 NLEPVKMPEAG 107
 
Name Accession Description Interval E-value
Piwi_piwi-like_Euk cd04658
Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found ...
 401-823 0e+00

Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related gene-silencing mechanisms mediated by short RNA molecules, including siRNAs, miRNAs, and heterochromatin-related guide RNAs. The mechanism in Piwi is believed to be similar to that in Argonaute, the central component of the RNA-induced silencing complex (RISC). The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the 5' anchoring of the guide RNA and the other, the catalytic site for slicing.


Pssm-ID: 240016 [Multi-domain]  Cd Length: 448  Bit Score: 624.29  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 401 NVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKtFDYNPQFADWSK 480
Cdd:cd04658    1 NLMKELAEHTKLNPKERYDTIRQFIQRIQKNPSVQELLKKWGIELDSNPLKIQGRVLPPEQIIMGNV-FVYANSNADWKR 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 481 ETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEV-DDRTEAYLRVLQQKVTADTQIVVCL 559
Cdd:cd04658   80 EIRNQPLYDAVNLNNWVLIYPSRDQREAESFLQTLKQVAGPMGIQISPPKIIKVkDDRIETYIRALKDAFRSDPQLVVII 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 560 LSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIP---LKLVMIVGIDCYHD 636
Cdd:cd04658  160 LPGNKKDLYDAIKKFCCVECPVPSQVITSRTLKKKKNLRSIASKIALQINAKLGGIPWTVEIPpfiLKNTMIVGIDVYHD 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 637 MTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQEL-VDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNY 715
Cdd:cd04658  240 TITKKKSVVGFVASLNKSITKWFSKYISQVRGQEEiIDSLGKSMKKALKAYKKENKKLPSRIIIYRDGVGDGQLKKVKEY 319

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 716 EVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYN 795
Cdd:cd04658  320 EVPQIKKAIKQYSENYSPKLAYIVVNKRINTRFFNQGGNNFSNPPPGTVVDSEITKPEWYDFFLVSQSVRQGTVTPTHYN 399

                        410       420
                 ....*....|....*....|....*...
gi 300244501 796 VIYDNSGLKPDHIQRLTYKLCHIYYNWP 823
Cdd:cd04658  400 VLYDTTGLKPDHLQRLTYKLCHLYYNWS 427
Piwi pfam02171
Piwi domain; This domain is found in the protein Piwi and its relatives. The function of this ...
 555-827 6.19e-117

Piwi domain; This domain is found in the protein Piwi and its relatives. The function of this domain is the dsRNA guided hydrolysis of ssRNA. Determination of the crystal structure of Argonaute reveals that PIWI is an RNase H domain, and identifies Argonaute as Slicer, the enzyme that cleaves mRNA in the RNAi RISC complex. In addition, Mg+2 dependence and production of 3'-OH and 5' phosphate products are shared characteriztics of RNaseH and RISC. The PIWI domain core has a tertiary structure belonging to the RNase H family of enzymes. RNase H fold proteins all have a five-stranded mixed beta-sheet surrounded by helices. By analogy to RNase H enzymes which cleave single-stranded RNA guided by the DNA strand in an RNA/DNA hybrid, the PIWI domain can be inferred to cleave single-stranded RNA, for example mRNA, guided by double stranded siRNA.


Pssm-ID: 396649  Cd Length: 296  Bit Score: 356.26  E-value: 6.19e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501  555 IVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTvMAIATKIALQMNCKMGGE-LWRVDIPLKLVMIVGIDC 633
Cdd:pfam02171   1 LILVILPEKNKDLYHSIKKYLETDLGIPSQCILSKTILKRTL-KQTLTNVLLKINVKLGGInYWIVEIKPKVDVIIGFDI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501  634 YHDM--TAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKT 711
Cdd:pfam02171  80 SHGTagTDDNPSVAAVVASFDKGNSRYFGTVRTQASGQELLEPLKDIIKELLRSFQKSSRKKPERIIVYRDGVSEGQFPQ 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501  712 LVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSG-GRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVS 790
Cdd:pfam02171 160 VLNYEVNQIKEACKSLGPGYNPKLTVIVVQKRHHTRFFANDKpDGDQNPPPGTVVDDVITLPEYYDFYLCSHAGLQGTVK 239

                         250       260       270
                  ....*....|....*....|....*....|....*..
gi 300244501  791 PTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPVSAS 827
Cdd:pfam02171 240 PTHYTVLYDEIGLSADELQNLTYKLCHMYYRSTRPIS 276
Piwi smart00950
This domain is found in the protein Piwi and its relatives; The function of this domain is the ...
 555-827 7.89e-111

This domain is found in the protein Piwi and its relatives; The function of this domain is the dsRNA guided hydrolysis of ssRNA. Determination of the crystal structure of Argonaute reveals that PIWI is an RNase H domain, and identifies Argonaute as Slicer, the enzyme that cleaves mRNA in the RNAi RISC complex.. In addition, Mg+2 dependence and production of 3'-OH and 5' phosphate products are shared characteristics of RNaseH and RISC. The PIWI domain core has a tertiary structure belonging to the RNase H family of enzymes. RNase H fold proteins all have a five-stranded mixed beta-sheet surrounded by helices. By analogy to RNase H enzymes which cleave single-stranded RNA guided by the DNA strand in an RNA/DNA hybrid, the PIWI domain can be inferred to cleave single-stranded RNA, for example mRNA, guided by double stranded siRNA.


Pssm-ID: 214930  Cd Length: 301  Bit Score: 340.85  E-value: 7.89e-111
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   555 IVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTL---GKQQTVMAIATKIALQMNCKMGGELWRVD---IPLKLVMI 628
Cdd:smart00950   2 IVVILPGEKKTDLYHEIKKYLETKLGVPTQCVQAKTLdkvSKRRKLKQYLTNVALKINAKLGGINWVLDvppIPLKPTLI 81

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   629 VGIDCYHDMTAGRRSIAGFVASINeGMTRWFSRCIFQD-RGQELVDGLKVCLQAALRAW-NSCNEYMPSRIIVYRDGVGD 706
Cdd:smart00950  82 IGIDVSHPSAGKGGSVAPSVAAFV-ASGNYLSGNFYQAfVREQGSRQLKEILREALKKYyKSNRKRLPDRIVVYRDGVSE 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   707 GQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRS 786
Cdd:smart00950 161 GQFKQVLEYEVKAIKKACKELGPDYKPKLTVIVVQKRHHTRFFPEDGNGRVNVPPGTVVDSVITSPEWYDFYLVSHAGLQ 240

                          250       260       270       280
                   ....*....|....*....|....*....|....*....|.
gi 300244501   787 GSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPVSAS 827
Cdd:smart00950 241 GTARPTHYTVLYDEGNLDPDELQRLTYKLCHLYYRSTRPVS 281
Piwi_ago-like cd04657
Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of ...
 438-819 2.29e-78

Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the 5' anchoring of the guide RNA and the other, the catalytic site for slicing.


Pssm-ID: 240015 [Multi-domain]  Cd Length: 426  Bit Score: 259.85  E-value: 2.29e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 438 LRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWskETRGAPLISVKPLDNWLLIY---TRRNYEAANSL--- 511
Cdd:cd04657    3 LKEFGISVSKEMITVPGRVLPPPKLKYGDSSKTVPPRNGSW--NLRGKKFLEGGPIRSWAVLNfagPRRSREERADLrnf 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 512 IQNLFKVTPAMGMQMRkAIMIEVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKylCTDCPT--PSQCVVAR 589
Cdd:cd04657   81 VDQLVKTVIGAGINIT-TAIASVEGRVEELFAKLKQAKGEGPQLVLVILPKKDSDIYGRIKR--LADTELgiHTQCVLAK 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 590 TLGKQQTvMAIATKIALQMNCKMGGELWRVD------IPLKLVMIVGIDCYH---DMTAGRRSIAGFVASINEGMTRWFS 660
Cdd:cd04657  158 KVTKKGN-PQYFANVALKINLKLGGINHSLEpdirplLTKEPTMVLGADVTHpspGDPAGAPSIAAVVASVDWHLAQYPA 236

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 661 RCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVV 740
Cdd:cd04657  237 SVRLQSHRQEIIDDLESMVRELLRAFKKATGKLPERIIYYRDGVSEGQFAQVLNEELPAIRKACAKLYPGYKPKITFIVV 316

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 741 KKRVNTRFFAQSG----GRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLC 816
Cdd:cd04657  317 QKRHHTRFFPTDEddadGKNGNVPPGTVVDRGITHPREFDFYLCSHAGIQGTARPTHYHVLWDEIGFTADELQTLTYNLC 396


                 ...
gi 300244501 817 HIY 819
Cdd:cd04657  397 YTY 399
Piwi-like cd02826
Piwi-like: PIWI domain. Domain found in proteins involved in RNA silencing. RNA silencing ...
 450-823 2.27e-69

Piwi-like: PIWI domain. Domain found in proteins involved in RNA silencing. RNA silencing refers to a group of related gene-silencing mechanisms mediated by short RNA molecules, including siRNAs, miRNAs, and heterochromatin-related guide RNAs. The central component of the RNA-induced silencing complex (RISC) and related complexes is Argonaute. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the 5' anchoring of the guide RNA and the other, the catalytic site for slicing. This domain is also found in closely related proteins, including the Piwi subfamily, where it is believed to perform a crucial role in germline cells, via a similar mechanism.


Pssm-ID: 239208 [Multi-domain]  Cd Length: 393  Bit Score: 234.59  E-value: 2.27e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 450 LSFSGRILQTEKIhqGGKTFDYNPQFADWSKETRGaplisvkplDNWLLIYTRrnYEAANSLIQNLFKVTPAMGMQMR-- 527
Cdd:cd02826    3 LILKGRVLPKPQI--LFKNKFLRNIGPFEKPAKIT---------NPVAVIAFR--NEEVDDLVKRLADACRQLGMKIKei 69

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 528 --KAIMIEVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDcPTPSQCVVARTLGKQQTVMAIATKIA 605
Cdd:cd02826   70 piVSWIEDLNNSFKDLKSVFKNAIKAGVQLVIFILKEKKPPLHDEIKRLEAKS-DIPSQVIQLKTAKKMRRLKQTLDNLL 148

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 606 LQMNCKMGGELWRVDIPLKL---VMIVGIDCYH---DMTAGRRSIAGFVASINEGM---TRWFSRCIFQDRGQELVDGLK 676
Cdd:cd02826  149 RKVNSKLGGINYILDSPVKLfksDIFIGFDVSHpdrRTVNGGPSAVGFAANLSNHTflgGFLYVQPSREVKLQDLGEVIK 228

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 677 VCLQAALRawnSCNEYMPSRIIVYRDGVGDGQLKtLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFA-QSGGR 755
Cdd:cd02826  229 KCLDGFKK---STGEGLPEKIVIYRDGVSEGEFK-RVKEEVEEIIKEACEIEESYRPKLVIIVVQKRHNTRFFPnEKNGG 304

                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 300244501 756 LQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWP 823
Cdd:cd02826  305 VQNPEPGTVVDHTITSPGLSEFYLASHVARQGTVKPTKYTVVFNDKNWSLNELEILTYILCLTHQNVY 372
PAZ smart00949
This domain is named PAZ after the proteins Piwi Argonaut and Zwille; This domain is found in ...
 277-415 1.88e-64

This domain is named PAZ after the proteins Piwi Argonaut and Zwille; This domain is found in two families of proteins that are involved in post-transcriptional gene silencing. These are the Piwi family and the Dicer family, that includes the Carpel factory protein. The function of the domains is unknown but has been suggested to mediate complex formation between proteins of the Piwi and Dicer families by hetero-dimerisation. The three-dimensional structure of this domain has been solved. The PAZ domain is composed of two subdomains. One subdomain is similar to the OB fold, albeit with a different topology. The OB-fold is well known as a single-stranded nucleic acid binding fold. The second subdomain is composed of a beta-hairpin followed by an alpha-helix. The PAZ domains shows low-affinity nucleic acid binding and appears to interact with the 3' ends of single-stranded regions of RNA in the cleft between the two subdomains. PAZ can bind the characteristic two-base 3' overhangs of siRNAs, indicating that although PAZ may not be a primary nucleic acid binding site in Dicer or RISC, it may contribute to the specific and productive incorporation of siRNAs and miRNAs into the RNAi pathway.


Pssm-ID: 198017  Cd Length: 138  Bit Score: 211.76  E-value: 1.88e-64
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   277 ETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITD 356
Cdd:smart00949   1 ETVLDFMRQLPSQGNRSNFQDRCAKDLKGLIVLTRYNNKTYRIDDIDWNLAPKSTFEKSDGSEITFVEYYKQKYNITIRD 80

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 300244501   357 LKQPVLVSQPKRRRGPGGTlPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPE 415
Cdd:smart00949  81 PNQPLLVSRPKRRRNQNGK-GEPVLLPPELCFITGLTDRMRKDFMLMKSIADRTRLSPL 138
PAZ_piwi_like cd02845
PAZ domain, Piwi_like subfamily. In multi-cellular organisms, the Piwi protein appears to be ...
 276-393 1.73e-62

PAZ domain, Piwi_like subfamily. In multi-cellular organisms, the Piwi protein appears to be essential for the maintenance of germline stem cells. In the Drosophila male germline, Piwi was shown to be involved in the silencing of retrotransposons in the male gametes. The Piwi proteins share their domain architecture with other members of the argonaute family. The PAZ domain has been named after the proteins Piwi, Argonaut, and Zwille. PAZ is found in two families of proteins that are essential components of RNA-mediated gene-silencing pathways, including RNA interference, the Piwi and Dicer families. PAZ functions as a nucleic acid binding domain, with a strong preference for single-stranded nucleic acids (RNA or DNA) or RNA duplexes with single-stranded 3' overhangs. It has been suggested that the PAZ domain provides a unique mode for the recognition of the two 3'-terminal nucleotides in single-stranded nucleic acids and buries the 3' OH group, and that it might recognize characteristic 3' overhangs in siRNAs within RISC (RNA-induced silencing) and other complexes.


Pssm-ID: 239211  Cd Length: 117  Bit Score: 205.57  E-value: 1.73e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 276 SETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEIT 355
Cdd:cd02845    1 STTVLDRMHKLYRQETDERFREECEKELIGSIVLTRYNNKTYRIDDIDFDKTPLSTFKKSDGTEITFVEYYKKQYNIEIT 80

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 300244501 356 DLKQPVLVSQPKRRRGPGGTlPGPAMLIPELCYLTGLT 393
Cdd:cd02845   81 DLNQPLLVSRPKRRDPRGGE-KEPIYLIPELCFLTGLT 117
PAZ pfam02170
PAZ domain; This domain is named PAZ after the proteins Piwi Argonaut and Zwille. This domain ...
 280-413 1.83e-44

PAZ domain; This domain is named PAZ after the proteins Piwi Argonaut and Zwille. This domain is found in two families of proteins that are involved in post-transcriptional gene silencing. These are the Piwi family and the Dicer family, that includes the Carpel factory protein. The function of the domains is unknown but has been suggested to mediate complex formation between proteins of the Piwi and Dicer families by hetero-dimerization. The three-dimensional structure of this domain has been solved. The PAZ domain is composed of two subdomains. One subdomain is similar to the OB fold, albeit with a different topology. The OB-fold is well known as a single-stranded nucleic acid binding fold. The second subdomain is composed of a beta-hairpin followed by an alpha-helix. The PAZ domains shows low-affinity nucleic acid binding and appears to interact with the 3' ends of single-stranded regions of RNA in the cleft between the two subdomains. PAZ can bind the characteriztic two-base 3' overhangs of siRNAs, indicating that although PAZ may not be a primary nucleic acid binding site in Dicer or RISC, it may contribute to the specific and productive incorporation of siRNAs and miRNAs into the RNAi pathway.


Pssm-ID: 460472  Cd Length: 123  Bit Score: 156.20  E-value: 1.83e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501  280 LDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNN-KTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLK 358
Cdd:pfam02170   1 LDFLKRLQQQKDRRDFRKEAKKALKGLKVYTTYNNpRTYRIDGITFDPTPESTFPLKDGKEITVVDYFKKKYNIDLKYPD 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 300244501  359 QPVLVSQPKRRRgpggtlpgpAMLIPELCYltgLTDKMRNDFNVMKDLAVHTRLT 413
Cdd:pfam02170  81 QPLLLVGKKRPK---------VYLPPELCN---LVDGQRYTKKLMPSIAQRTRLL 123
PLN03202 PLN03202
protein argonaute; Provisional
 251-819 3.11e-39

protein argonaute; Provisional


Pssm-ID: 215631 [Multi-domain]  Cd Length: 900  Bit Score: 156.80  E-value: 3.11e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 251 GFTTSILQYENSIMLCTDVSHK-VLRSETVLDFMFNfyHQTEEHKFQ---EQVSKELIGLVVLTKYNNKTYRVddIDWDQ 326
Cdd:PLN03202 241 GFHSSFRTTQGGLSLNIDVSTTmIVQPGPVVDFLIA--NQNVRDPFQidwSKAKRMLKNLRVKVSPSNQEYKI--TGLSE 316

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 327 NP--KSTF--KKADGS-------EVSFLEYYRKQYNQEIT---DLkqPVL-VSQPKRrrgpggtlpgPAMLIPELCYLTG 391
Cdd:PLN03202 317 KPckEQTFslKQRNGNgnevetvEITVYDYFVKHRGIELRysgDL--PCInVGKPKR----------PTYFPIELCSLVS 384

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 392 LTdkmrndfNVMKDLAVHTR--LTPEQRQR--EVGRLIDYIHKNDNVQRE--LRDWGLSFDSNLLSFSGRILQTEKIhQG 465
Cdd:PLN03202 385 LQ-------RYTKALSTLQRssLVEKSRQKpqERMKVLTDALKSSNYDADpmLRSCGISISSQFTQVEGRVLPAPKL-KV 456

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 466 GKTFDYNPQFADWSKETRgaPLISVKPLDNWLLIytrrNYEA---ANSLIQNLFKVTPAMGM-------------QMRKA 529
Cdd:PLN03202 457 GNGEDFFPRNGRWNFNNK--KLVEPTKIERWAVV----NFSArcdIRHLVRDLIKCGEMKGInieppfdvfeenpQFRRA 530

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 530 -IMIEVDDRTEAylrvLQQKVTADTQIVVCLLSsNRK--DKYDAIKKYLCTDCPTPSQCVVARTLGKQQTvmaiaTKIAL 606
Cdd:PLN03202 531 pPPVRVEKMFEQ----IQSKLPGPPQFLLCILP-ERKnsDIYGPWKKKNLSEFGIVTQCIAPTRVNDQYL-----TNVLL 600

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 607 QMNCKMGG--ELWRVD----IPL--KL-VMIVGIDCYHDmTAGRR---SIAGFVASINEGMTRWFSRCI-FQDRGQELVD 673
Cdd:PLN03202 601 KINAKLGGlnSLLAIEhspsIPLvsKVpTIILGMDVSHG-SPGQSdvpSIAAVVSSRQWPLISRYRASVrTQSPKVEMID 679

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 674 GL----------KVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKR 743
Cdd:PLN03202 680 SLfkpvgdkdddGIIRELLLDFYTSSGKRKPEQIIIFRDGVSESQFNQVLNIELDQIIEACKFLDESWSPKFTVIVAQKN 759

                        570       580       590       600       610       620       630
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 300244501 744 VNTRFFaQSGGRlQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIY 819
Cdd:PLN03202 760 HHTKFF-QAGSP-DNVPPGTVVDNKICHPRNNDFYMCAHAGMIGTTRPTHYHVLLDEIGFSADDLQELVHSLSYVY 833
GAGE pfam05831
GAGE protein; This family consists of several GAGE and XAGE proteins which are found ...
 16-106 2.47e-32

GAGE protein; This family consists of several GAGE and XAGE proteins which are found exclusively in humans. The function of this family is unknown although they have been implicated in human cancers.


Pssm-ID: 461753  Cd Length: 107  Bit Score: 120.95  E-value: 2.47e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501   16 QETAQLVGSTASQQPGYIQPRPQPPPAEGELFGRGRQRGTaGGTAKSQGLQISAGFQELSLAERGGRRRDFHDLGVNTRQ 95
Cdd:pfam05831  18 QESSQLVGPVVAQQPSDEQPQQEEPPTESQDITPGQERED-EGASAIQGPELEADLQELALEKTGGERGDGPDVKGKTLP 96

                          90
                  ....*....|.
gi 300244501   96 NLDHVKESKTG 106
Cdd:pfam05831  97 NLEPVKMPEAG 107
PAZ cd02825
PAZ domain, named PAZ after the proteins Piwi Argonaut and Zwille. PAZ is found in two ...
 278-390 7.04e-17

PAZ domain, named PAZ after the proteins Piwi Argonaut and Zwille. PAZ is found in two families of proteins that are essential components of RNA-mediated gene-silencing pathways, including RNA interference, the piwi and Dicer families. PAZ functions as a nucleic-acid binding domain, with a strong preference for single-stranded nucleic acids (RNA or DNA) or RNA duplexes with single-stranded 3' overhangs. It has been suggested that the PAZ domain provides a unique mode for the recognition of the two 3'-terminal nucleotides in single-stranded nucleic acids and buries the 3' OH group, and that it might recognize characteristic 3' overhangs in siRNAs within RISC (RNA-induced silencing) and other complexes. This parent model also contains structures of an archaeal PAZ domain.


Pssm-ID: 239207  Cd Length: 115  Bit Score: 77.12  E-value: 7.04e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 278 TVLDFMFNFYHQTEEHK-----FQEQVSKELIGLVVLTKYN--NKTYRVDDIDWDQNPkSTFKKADGSEVSFLEYYRKQY 350
Cdd:cd02825    3 PVIETMCKFPKDREIDTplldsPREEFTKELKGLKVEDTHNplNRVYRPDGETRLKAP-SQLKHSDGKEITFADYFKERY 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 300244501 351 NQEITDLKQPVLVSQPKRRrgpggtLPGPAMLIPELCYLT 390
Cdd:cd02825   82 NLTLTDLNQPLLIVKFSSK------KSYSILLPPELCVIT 115
Piwi_piwi-like_ProArk cd04659
Piwi_piwi-like_ProArk: PIWI domain, Piwi-like subfamily found in Archaea and Bacteria. RNA ...
 538-765 9.40e-09

Piwi_piwi-like_ProArk: PIWI domain, Piwi-like subfamily found in Archaea and Bacteria. RNA silencing refers to a group of related gene-silencing mechanisms mediated by short RNA molecules, including siRNAs, miRNAs, and heterochromatin-related guide RNAs. The central component of the RNA-induced silencing complex (RISC) and related complexes is Argonaute. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the 5' anchoring of the guide RNA and the other, the catalytic site for slicing. This domain is also found in closely related proteins, including the Piwi subfamily, where it is believed to perform a crucial role in germline cells, via a similar mechanism.


Pssm-ID: 240017 [Multi-domain]  Cd Length: 404  Bit Score: 58.55  E-value: 9.40e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 538 TEAYLRVLQQKVTAdtqIVVCLLSSNRK-----DKYDAIKKYLcTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKM 612
Cdd:cd04659  100 VDLALSESSQGVDV---VIVVLPEDLKElpeefDLYDRLKAKL-LRLGIPTQFVREDTLKNRQDLAYVAWNLALALYAKL 175

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 613 GGELWRVD-IPLKLVMIVGIDcYHDMTAGRRSIAGFVASINEGMTRWFSR-----CIFQDRGQELV-DGLKVCLQAALRa 685
Cdd:cd04659  176 GGIPWKLDaDSDPADLYIGIG-FARSRDGEVRVTGCAQVFDSDGLGLILRgapieEPTEDRSPADLkDLLKRVLEGYRE- 253

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 686 wnSCNEYMPSRIIVYRDGvgdgqlkTLVNYEVPQFLDCLKSIGRgynpRLTVIVVKKRVNTRFFAQSG-GRLQNPLPGTV 764
Cdd:cd04659  254 --SHRGRDPKRLVLHKDG-------RFTDEEIEGLKEALEELGI----KVDLVEVIKSGPHRLFRFGTyPNGFPPRRGTY 320


                 .
gi 300244501 765 I 765
Cdd:cd04659  321 V 321
PAZ_CAF_like cd02844
PAZ domain, CAF_like subfamily. CAF (for carpel factory) is a plant homolog of Dicer. CAF has ...
 299-387 1.97e-06

PAZ domain, CAF_like subfamily. CAF (for carpel factory) is a plant homolog of Dicer. CAF has been implicated in flower morphogenesis and in early Arabidopsis development and might function through posttranscriptional regulation of specific mRNA molecules. PAZ domains are named after the proteins Piwi, Argonaut, and Zwille. PAZ is found in two families of proteins that are essential components of RNA-mediated gene-silencing pathways, including RNA interference, the Piwi and Dicer families. PAZ functions as a nucleic-acid binding domain, with a strong preference for single-stranded nucleic acids (RNA or DNA) or RNA duplexes with single-stranded 3' overhangs. It has been suggested that the PAZ domain provides a unique mode for the recognition of the two 3'-terminal nucleotides in single-stranded nucleic acids and buries the 3' OH group, and that it might recognize characteristic 3' overhangs in siRNAs within RISC (RNA-induced silencing) and other complexes.


Pssm-ID: 239210  Cd Length: 135  Bit Score: 47.80  E-value: 1.97e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 299 VSKELIGLVVLTKYNNKTYrVDDIDWDQNPKSTFKKADGSEV-SFLEYYRKQYNQEITDLKQPVLVSQP----------- 366
Cdd:cd02844   27 CACDLKGSVVTAPHNGRFY-VISGILDLNANSSFPGKEGLGYaTYAEYFKEKYGIVLNHPNQPLLKGKQifnlhnllhnr 105

                         90       100
                 ....*....|....*....|....
gi 300244501 367 KRRRGPGGTLPGP---AMLIPELC 387
Cdd:cd02844  106 FEEKGESEEKEKDryfVELPPELC 129
PAZ_argonaute_like cd02846
PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex ...
 299-387 1.44e-04

PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. PAZ is found in two families of proteins that are essential components of RNA-mediated gene-silencing pathways, including RNA interference, the Piwi and Dicer families. PAZ functions as a nucleic acid binding domain, with a strong preference for single-stranded nucleic acids (RNA or DNA) or RNA duplexes with single-stranded 3' overhangs. It has been suggested that the PAZ domain provides a unique mode for the recognition of the two 3'-terminal nucleotides in single-stranded nucleic acids and buries the 3' OH group, and that it might recognize characteristic 3' overhangs in siRNAs within RISC (RNA-induced silencing) and other complexes.


Pssm-ID: 239212 [Multi-domain]  Cd Length: 114  Bit Score: 41.92  E-value: 1.44e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 299 VSKELIGLVVLTKY---NNKTYRVDDIDWDQNPKSTFKKADGS-EVSFLEYYRKQYNQEITDLKQPVLVSQPKRRrgpgg 374
Cdd:cd02846   28 LKKALKGLKVEVTHrgnTNRKYKIKGLSAEPASQQTFELKDGEkEISVADYFKEKYNIRLKYPNLPCLQVGRKGK----- 102

                         90
                 ....*....|...
gi 300244501 375 tlpgPAMLIPELC 387
Cdd:cd02846  103 ----PNYLPMELC 111
PAZ_dicer_like cd02843
PAZ domain, dicer_like subfamily. Dicer is an RNAse involved in cleaving dsRNA in the RNA ...
 307-362 2.42e-04

PAZ domain, dicer_like subfamily. Dicer is an RNAse involved in cleaving dsRNA in the RNA interference pathway. It generates dsRNAs which are approximately 20 bp long (siRNAs), which in turn target hydrolysis of homologous RNAs. PAZ domains are named after the proteins Piwi Argonaut and Zwille. PAZ is found in two families of proteins that are essential components of RNA-mediated gene-silencing pathways, including RNA interference, the piwi and Dicer families. PAZ functions as a nucleic-acid binding domain, with a strong preference for single-stranded nucleic acids (RNA or DNA) or RNA duplexes with single-stranded 3' overhangs. It has been suggested that the PAZ domain provides a unique mode for the recognition of the two 3'-terminal nucleotides in single-stranded nucleic acids and buries the 3' OH group, and that it might recognize characteristic 3' overhangs in siRNAs within RISC (RNA-induced silencing) and other complexes.


Pssm-ID: 239209  Cd Length: 122  Bit Score: 41.66  E-value: 2.42e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 300244501 307 VVLTKYNN----KTYRVDDIDWDQNPKSTFKKADGSevSFLEYYRKQYNQEITDLKQPVL 362
Cdd:cd02843   45 VVMPWYRNfdqpQYFYVAEICTDLRPLSKFPGPEYE--TFEEYYKKKYKLDIQNLNQPLL 102
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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