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Conserved domains on  [gi|304766677|ref|NP_001182156|]
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transient receptor potential cation channel subfamily M member 4 isoform 2 [Homo sapiens]

Protein Classification

transient-receptor-potential channel family protein; transient-receptor-potential channel protein( domain architecture ID 1904656)

transient-receptor-potential ion channel protein conducts cations such as calcium into cells; belongs to the Transient Receptor Family (TC. 1.A.4)| transient-receptor-potential ion channel protein conducts cations such as calcium into cells; belongs to the Transient Receptor Family (TC. 1.A.4)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
LSDAT_euk super family cl39032
SLOG in TRPM; Family in the SLOG superfamily, found in several eukaryotic channels including ...
 88-357 9.09e-54

SLOG in TRPM; Family in the SLOG superfamily, found in several eukaryotic channels including diverse ciliate channels and the TRPM class of animal ion channels. Positioned near the N-terminus of all TRPM channels, it is predicted to play a regulatory role for the channel in potentially recognizing a universal nucleotide or nucleotide-derived ligand.


The actual alignment was detected with superfamily member pfam18139:

Pssm-ID: 476852  Cd Length: 266  Bit Score: 188.56  E-value: 9.09e-54
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677    88 SNFLRLSDRTDPAAVYSLVTRTWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRRGLVRAAQSTGAWIVTGGLHTGIGRHVG 167
Cdd:pfam18139    1 AKYIRLSFDTDPEDLLHLMEKEWQLELPKLVISVHGGATNFELQPKLKRVFRKGLLKAAKTTGAWIITGGTNTGVMRHVG 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677   168 VAVRDHqmASTGGTKVVAMGVAPWGVVRNRDTLINPKGSFParYRWRGDPEDGVQfPLDYNYSAFFLVDDGTHGCLGGEN 247
Cdd:pfam18139   81 DALKDL--GSQSRRKIVTIGIAPWGIIKNREDLIGKDVVVP--YQTLGNPKSKLA-VLNNNHSHFLLVDDGTVGKYGAEI 155

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677   248 RFRLRLESYISQQKTgVGGTGIDIPVLLLLIDGD-EKMLTRIENATQA-QLPCLLVAGSGGAADCLAETLEDTLAPGSGG 325
Cdd:pfam18139  156 ILRRRLEKYISQQKI-HPRGGQGVPVVCVVVEGGpNTIKTVLEYVRDTpPVPVVVCDGSGRAADLLAFAHKYTHEDGQLP 234

                          250       260       270
                   ....*....|....*....|....*....|...
gi 304766677   326 AR-QGEARDRIRRFFPKGdlevlQAQVERIMTR 357
Cdd:pfam18139  235 SSvKEQLLSLIQKTFGYS-----QKQAEKLLKQ 262
TRPV super family cl40437
Transient Receptor Potential channel, Vanilloid subfamily (TRPV); The vanilloid TRP subfamily ...
 888-938 2.26e-04

Transient Receptor Potential channel, Vanilloid subfamily (TRPV); The vanilloid TRP subfamily (TRPV), named after the vanilloid receptor 1 (TRPV1), consists of six members: four thermo-sensing channels (TRPV1, TRPV2, TRPV3, and TRPV4) and two Ca2+ selective channels (TRPV5 and TRPV6). The calcium-selective channels TRPV5 and TRPV6 can be heterotetramers and are important for general Ca2+ homeostasis. All four channels within the TRPV1-4 group show temperature-invoked currents when expressed in heterologous cell systems, ranging from activation at ~25C for TRPV4 to ~52C for TRPV2. The structure of TRPV shows the typical topology features of all Transient Receptor Potential (TRP) ion channel family members, such as six transmembrane regions, a short hydrophobic stretch between transmembrane segments 5 and 6 and large intracellular N- and C-terminal domains. The TRP family consists of membrane proteins that function as ion channels that communicate between the cell and its environment, by a vast array of physical or chemical stimuli, including radiation (in the form of temperature, infrared ,or light) and pressure (osmotic or mechanical). TRP channels are formed by a tetrameric complex of channel subunits. Based on sequence identity, the mammalian TRP channel family is classified into six subfamilies, with significant sequence similarity within the transmembrane domains, but very low similarity in their N- and C-terminal cytoplasmic regions. The six subfamilies are named based on their first member: TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPA (ankyrin), TRPML (mucolipin), and TRPP (polycystic).


The actual alignment was detected with superfamily member TIGR00870:

Pssm-ID: 454755 [Multi-domain]  Cd Length: 743  Bit Score: 45.46  E-value: 2.26e-04
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|..
gi 304766677   888 ILLVNLLIAMFSYTFGKVQGNSDLYWKAQRYRLIRE-FHSRPALAPPFIVIS 938
Cdd:TIGR00870  601 ILLLNMLIAMMGNTYQLIADDADEEWKFQRAKLWMSyEREGGTCPPPFNIIP 652
 
Name Accession Description Interval E-value
LSDAT_euk pfam18139
SLOG in TRPM; Family in the SLOG superfamily, found in several eukaryotic channels including ...
 88-357 9.09e-54

SLOG in TRPM; Family in the SLOG superfamily, found in several eukaryotic channels including diverse ciliate channels and the TRPM class of animal ion channels. Positioned near the N-terminus of all TRPM channels, it is predicted to play a regulatory role for the channel in potentially recognizing a universal nucleotide or nucleotide-derived ligand.


Pssm-ID: 465665  Cd Length: 266  Bit Score: 188.56  E-value: 9.09e-54
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677    88 SNFLRLSDRTDPAAVYSLVTRTWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRRGLVRAAQSTGAWIVTGGLHTGIGRHVG 167
Cdd:pfam18139    1 AKYIRLSFDTDPEDLLHLMEKEWQLELPKLVISVHGGATNFELQPKLKRVFRKGLLKAAKTTGAWIITGGTNTGVMRHVG 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677   168 VAVRDHqmASTGGTKVVAMGVAPWGVVRNRDTLINPKGSFParYRWRGDPEDGVQfPLDYNYSAFFLVDDGTHGCLGGEN 247
Cdd:pfam18139   81 DALKDL--GSQSRRKIVTIGIAPWGIIKNREDLIGKDVVVP--YQTLGNPKSKLA-VLNNNHSHFLLVDDGTVGKYGAEI 155

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677   248 RFRLRLESYISQQKTgVGGTGIDIPVLLLLIDGD-EKMLTRIENATQA-QLPCLLVAGSGGAADCLAETLEDTLAPGSGG 325
Cdd:pfam18139  156 ILRRRLEKYISQQKI-HPRGGQGVPVVCVVVEGGpNTIKTVLEYVRDTpPVPVVVCDGSGRAADLLAFAHKYTHEDGQLP 234

                          250       260       270
                   ....*....|....*....|....*....|...
gi 304766677   326 AR-QGEARDRIRRFFPKGdlevlQAQVERIMTR 357
Cdd:pfam18139  235 SSvKEQLLSLIQKTFGYS-----QKQAEKLLKQ 262
trp TIGR00870
transient-receptor-potential calcium channel protein; The Transient Receptor Potential Ca2+ ...
 888-938 2.26e-04

transient-receptor-potential calcium channel protein; The Transient Receptor Potential Ca2+ Channel (TRP-CC) Family (TC. 1.A.4)The TRP-CC family has also been called the store-operated calcium channel (SOC) family. The prototypical members include the Drosophila retinal proteinsTRP and TRPL (Montell and Rubin, 1989; Hardie and Minke, 1993). SOC members of the family mediate the entry of extracellular Ca2+ into cells in responseto depletion of intracellular Ca2+ stores (Clapham, 1996) and agonist stimulated production of inositol-1,4,5 trisphosphate (IP3). One member of the TRP-CCfamily, mammalian Htrp3, has been shown to form a tight complex with the IP3 receptor (TC #1.A.3.2.1). This interaction is apparently required for IP3 tostimulate Ca2+ release via Htrp3. The vanilloid receptor subtype 1 (VR1), which is the receptor for capsaicin (the ?hot? ingredient in chili peppers) and servesas a heat-activated ion channel in the pain pathway (Caterina et al., 1997), is also a member of this family. The stretch-inhibitable non-selective cation channel(SIC) is identical to the vanilloid receptor throughout all of its first 700 residues, but it exhibits a different sequence in its last 100 residues. VR1 and SICtransport monovalent cations as well as Ca2+. VR1 is about 10x more permeable to Ca2+ than to monovalent ions. Ca2+ overload probably causes cell deathafter chronic exposure to capsaicin. (McCleskey and Gold, 1999). [Transport and binding proteins, Cations and iron carrying compounds]


Pssm-ID: 273311 [Multi-domain]  Cd Length: 743  Bit Score: 45.46  E-value: 2.26e-04
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|..
gi 304766677   888 ILLVNLLIAMFSYTFGKVQGNSDLYWKAQRYRLIRE-FHSRPALAPPFIVIS 938
Cdd:TIGR00870  601 ILLLNMLIAMMGNTYQLIADDADEEWKFQRAKLWMSyEREGGTCPPPFNIIP 652
 
Name Accession Description Interval E-value
LSDAT_euk pfam18139
SLOG in TRPM; Family in the SLOG superfamily, found in several eukaryotic channels including ...
 88-357 9.09e-54

SLOG in TRPM; Family in the SLOG superfamily, found in several eukaryotic channels including diverse ciliate channels and the TRPM class of animal ion channels. Positioned near the N-terminus of all TRPM channels, it is predicted to play a regulatory role for the channel in potentially recognizing a universal nucleotide or nucleotide-derived ligand.


Pssm-ID: 465665  Cd Length: 266  Bit Score: 188.56  E-value: 9.09e-54
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677    88 SNFLRLSDRTDPAAVYSLVTRTWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRRGLVRAAQSTGAWIVTGGLHTGIGRHVG 167
Cdd:pfam18139    1 AKYIRLSFDTDPEDLLHLMEKEWQLELPKLVISVHGGATNFELQPKLKRVFRKGLLKAAKTTGAWIITGGTNTGVMRHVG 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677   168 VAVRDHqmASTGGTKVVAMGVAPWGVVRNRDTLINPKGSFParYRWRGDPEDGVQfPLDYNYSAFFLVDDGTHGCLGGEN 247
Cdd:pfam18139   81 DALKDL--GSQSRRKIVTIGIAPWGIIKNREDLIGKDVVVP--YQTLGNPKSKLA-VLNNNHSHFLLVDDGTVGKYGAEI 155

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677   248 RFRLRLESYISQQKTgVGGTGIDIPVLLLLIDGD-EKMLTRIENATQA-QLPCLLVAGSGGAADCLAETLEDTLAPGSGG 325
Cdd:pfam18139  156 ILRRRLEKYISQQKI-HPRGGQGVPVVCVVVEGGpNTIKTVLEYVRDTpPVPVVVCDGSGRAADLLAFAHKYTHEDGQLP 234

                          250       260       270
                   ....*....|....*....|....*....|...
gi 304766677   326 AR-QGEARDRIRRFFPKGdlevlQAQVERIMTR 357
Cdd:pfam18139  235 SSvKEQLLSLIQKTFGYS-----QKQAEKLLKQ 262
LSDAT_prok pfam18171
SLOG in TRPM, prokaryote; Family in the SLOG superfamily, fused to or operonically associating ...
 118-312 3.35e-17

SLOG in TRPM, prokaryote; Family in the SLOG superfamily, fused to or operonically associating with SLATT domain in diverse prokaryotes. Predicted to function as ligand sensor in conjunction with the SLATT transmembrane domain.


Pssm-ID: 408001  Cd Length: 194  Bit Score: 80.80  E-value: 3.35e-17
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677   118 VVSVLGGSGG--PVLQTWLQDLLRRGLVRAAQSTGAWIVTGGLHTGIGRHVGVAVrdhqmASTGGT-KVVamGVAPWGVV 194
Cdd:pfam18171    3 VLVLVGGASGldPELAARLLPLFRAVLAPAAEALGAVVIDGGTDTGVMRLMGRAR-----AATDGRfPLV--GVAPSGTV 75

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 304766677   195 RNRDTLINPKGsfparyrwrgdpedgvQFPLDYNYSAFFLVDdGTHgcLGGENRFRLRLESYISQQKtgvggtgidiPVL 274
Cdd:pfam18171   76 AYPDEPTPTDD----------------AARLEPHHTHFVLVP-GSR--WGDESPWLARLATAIAGGR----------PSV 126

                          170       180       190
                   ....*....|....*....|....*....|....*...
gi 304766677   275 LLLIDGDEKMLTRIENATQAQLPCLLVAGSGGAADCLA 312
Cdd:pfam18171  127 TLLVNGGAITRLDIRASLAAGRPLLVLAGSGRLADEIA 164
trp TIGR00870
transient-receptor-potential calcium channel protein; The Transient Receptor Potential Ca2+ ...
 888-938 2.26e-04

transient-receptor-potential calcium channel protein; The Transient Receptor Potential Ca2+ Channel (TRP-CC) Family (TC. 1.A.4)The TRP-CC family has also been called the store-operated calcium channel (SOC) family. The prototypical members include the Drosophila retinal proteinsTRP and TRPL (Montell and Rubin, 1989; Hardie and Minke, 1993). SOC members of the family mediate the entry of extracellular Ca2+ into cells in responseto depletion of intracellular Ca2+ stores (Clapham, 1996) and agonist stimulated production of inositol-1,4,5 trisphosphate (IP3). One member of the TRP-CCfamily, mammalian Htrp3, has been shown to form a tight complex with the IP3 receptor (TC #1.A.3.2.1). This interaction is apparently required for IP3 tostimulate Ca2+ release via Htrp3. The vanilloid receptor subtype 1 (VR1), which is the receptor for capsaicin (the ?hot? ingredient in chili peppers) and servesas a heat-activated ion channel in the pain pathway (Caterina et al., 1997), is also a member of this family. The stretch-inhibitable non-selective cation channel(SIC) is identical to the vanilloid receptor throughout all of its first 700 residues, but it exhibits a different sequence in its last 100 residues. VR1 and SICtransport monovalent cations as well as Ca2+. VR1 is about 10x more permeable to Ca2+ than to monovalent ions. Ca2+ overload probably causes cell deathafter chronic exposure to capsaicin. (McCleskey and Gold, 1999). [Transport and binding proteins, Cations and iron carrying compounds]


Pssm-ID: 273311 [Multi-domain]  Cd Length: 743  Bit Score: 45.46  E-value: 2.26e-04
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|..
gi 304766677   888 ILLVNLLIAMFSYTFGKVQGNSDLYWKAQRYRLIRE-FHSRPALAPPFIVIS 938
Cdd:TIGR00870  601 ILLLNMLIAMMGNTYQLIADDADEEWKFQRAKLWMSyEREGGTCPPPFNIIP 652
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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