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Conserved domains on  [gi|546231697|ref|NP_001271195|]
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EF-hand calcium-binding domain-containing protein 11 isoform 2 [Homo sapiens]

Protein Classification

EF-hand domain-containing protein( domain architecture ID 12144783)

EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

CATH:  1.10.238.10
Gene Ontology:  GO:0005509
PubMed:  2479149|10191494
SCOP:  3001983

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
25-84 1.11e-05

EF-hand domain pair;


:

Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 40.70  E-value: 1.11e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 546231697   25 EVFKACDEDHKGYLSREDFKTAVVMLF-GYKPSKIEVDSVMSSINPNTSG-ILLEGFLNIVR 84
Cdd:pfam13499   6 EAFKLLDSDGDGYLDVEELKKLLRKLEeGEPLSDEEVEELFKEFDLDKDGrISFEEFLELYS 67
 
Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
25-84 1.11e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 40.70  E-value: 1.11e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 546231697   25 EVFKACDEDHKGYLSREDFKTAVVMLF-GYKPSKIEVDSVMSSINPNTSG-ILLEGFLNIVR 84
Cdd:pfam13499   6 EAFKLLDSDGDGYLDVEELKKLLRKLEeGEPLSDEEVEELFKEFDLDKDGrISFEEFLELYS 67
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
21-139 1.36e-05

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 42.09  E-value: 1.36e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231697  21 RKWVEVFKACDEDHKGYLSREDFKTAVVMLFGYKPSKIEVDSvmssinpnTSGILLEGFLNIVRKKKEAQRyRNEVRHIF 100
Cdd:COG5126    5 RKLDRRFDLLDADGDGVLERDDFEALFRRLWATLFSEADTDG--------DGRISREEFVAGMESLFEATV-EPFARAAF 75

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 546231697 101 TAFDTYYRGFLTLEDFKKAFRqvAPKLPERTVLEVFRGI 139
Cdd:COG5126   76 DLLDTDGDGKISADEFRRLLT--ALGVSEEEADELFARL 112
PTZ00183 PTZ00183
centrin; Provisional
 25-129 1.20e-04

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 39.67  E-value: 1.20e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231697  25 EVFKACDEDHKGYLSREDFKTAVVMLfGYKPSKIEVDSVMSSINPNTSGIL-LEGFLNIVRKKKEAQRYRNEVRHIFTAF 103
Cdd:PTZ00183  21 EAFDLFDTDGSGTIDPKELKVAMRSL-GFEPKKEEIKQMIADVDKDGSGKIdFEEFLDIMTKKLGERDPREEILKAFRLF 99

                         90       100
                 ....*....|....*....|....*.
gi 546231697 104 DTYYRGFLTLedfkKAFRQVAPKLPE 129
Cdd:PTZ00183 100 DDDKTGKISL----KNLKRVAKELGE 121
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 25-84 3.39e-04

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 36.76  E-value: 3.39e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 546231697  25 EVFKACDEDHKGYLSREDFKTAVVMLfGYKPSKIEVDSVMSSINPNTSGIL-LEGFLNIVR 84
Cdd:cd00051    4 EAFRLFDKDGDGTISADELKAALKSL-GEGLSEEEIDEMIREVDKDGDGKIdFEEFLELMA 63
 
Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
25-84 1.11e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 40.70  E-value: 1.11e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 546231697   25 EVFKACDEDHKGYLSREDFKTAVVMLF-GYKPSKIEVDSVMSSINPNTSG-ILLEGFLNIVR 84
Cdd:pfam13499   6 EAFKLLDSDGDGYLDVEELKKLLRKLEeGEPLSDEEVEELFKEFDLDKDGrISFEEFLELYS 67
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
21-139 1.36e-05

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 42.09  E-value: 1.36e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231697  21 RKWVEVFKACDEDHKGYLSREDFKTAVVMLFGYKPSKIEVDSvmssinpnTSGILLEGFLNIVRKKKEAQRyRNEVRHIF 100
Cdd:COG5126    5 RKLDRRFDLLDADGDGVLERDDFEALFRRLWATLFSEADTDG--------DGRISREEFVAGMESLFEATV-EPFARAAF 75

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 546231697 101 TAFDTYYRGFLTLEDFKKAFRqvAPKLPERTVLEVFRGI 139
Cdd:COG5126   76 DLLDTDGDGKISADEFRRLLT--ALGVSEEEADELFARL 112
PTZ00183 PTZ00183
centrin; Provisional
 25-129 1.20e-04

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 39.67  E-value: 1.20e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231697  25 EVFKACDEDHKGYLSREDFKTAVVMLfGYKPSKIEVDSVMSSINPNTSGIL-LEGFLNIVRKKKEAQRYRNEVRHIFTAF 103
Cdd:PTZ00183  21 EAFDLFDTDGSGTIDPKELKVAMRSL-GFEPKKEEIKQMIADVDKDGSGKIdFEEFLDIMTKKLGERDPREEILKAFRLF 99

                         90       100
                 ....*....|....*....|....*.
gi 546231697 104 DTYYRGFLTLedfkKAFRQVAPKLPE 129
Cdd:PTZ00183 100 DDDKTGKISL----KNLKRVAKELGE 121
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 25-84 3.39e-04

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 36.76  E-value: 3.39e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 546231697  25 EVFKACDEDHKGYLSREDFKTAVVMLfGYKPSKIEVDSVMSSINPNTSGIL-LEGFLNIVR 84
Cdd:cd00051    4 EAFRLFDKDGDGTISADELKAALKSL-GEGLSEEEIDEMIREVDKDGDGKIdFEEFLELMA 63
EFh_PI-PLC cd15898
EF-hand motif found in eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4. ...
 25-116 5.64e-04

EF-hand motif found in eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) isozymes; PI-PLC isozymes are signaling enzymes that hydrolyze the membrane phospholipids phosphatidylinositol-4,5-bisphosphate (PIP2) to generate two important second messengers in eukaryotic signal transduction cascades, Inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol (DAG). InsP3 triggers inflow of calcium from intracellular stores, while DAG, together with calcium, activates protein kinase C, which goes on to phosphorylate other molecules, leading to altered cellular activity. Calcium is required for the catalysis. This family corresponds to the four EF-hand motifs containing PI-PLC isozymes, including PI-PLC-beta (1-4), -gamma (1-2), -delta (1,3,4), -epsilon (1), -zeta (1), eta (1-2). Lower eukaryotes such as yeast and slime molds contain only delta-type isozymes. In contrast, other types of isoforms present in higher eukaryotes. This family also includes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (PLC1) from fungi. Some homologs from plants contain only two atypical EF-hand motifs and they are not included. All PI-PLC isozymes except sperm-specific PI-PLC-zeta share a core set of domains, including an N-terminal pleckstrin homology (PH) domain, four atypical EF-hand motifs, a PLC catalytic core, and a single C2 domain. PI-PLC-zeta lacks the PH domain. The PLC catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a highly degenerate linker sequence. Most of EF-hand motifs found in PI-PLCs consist of a helix-loop-helix structure, but lack residues critical to metal binding. Moreover, the EF-hand region of most of PI-PLCs may have an important regulatory function, but it has yet to be identified. However, PI-PLC-zeta is a key exception. It is responsible for Ca2+ oscillations in fertilized oocytes and exhibits a high sensitivity to Ca2+ mediated through its EF-hand domain. In addition, PI-PLC-eta2 shows a canonical EF-loop directing Ca2+-sensitivity and thus can amplify transient Ca2+ signals. Also it appears that PI-PLC-delta1 can regulate the binding of PH domain to PIP2 in a Ca2+-dependent manner through its functionally important EF-hand domains. PI-PLCs can be activated by a variety of extracellular ligands, such as growth factors, hormones, cytokines and lipids. Their activation has been implicated in tumorigenesis and/or metastasis linked to migration, proliferation, growth, inflammation, angiogenesis and actin cytoskeleton reorganization. PI-PLC-beta isozymes are activated by G-protein coupled receptor (GPCR) through different mechanisms. However, PI-PLC-gamma isozymes are activated by receptor tyrosine kinase (RTK), such as Rho and Ras GTPases. In contrast, PI-PLC-epsilon are activated by both GPCR and RTK. PI-PLC-delta1 and PLC-eta 1 are activated by GPCR-mediated calcium mobilization. The activation mechanism for PI-PLC-zeta remains unclear.


Pssm-ID: 320029 [Multi-domain]  Cd Length: 137  Bit Score: 37.65  E-value: 5.64e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231697  25 EVFKACDEDHKGYLSREDFktavVMLFGYKPSKIEVDSVMSSI-NPNTSGILLEGFLNIVRKKKEAQRYRNEVRHIFTAF 103
Cdd:cd15898   40 KLFKEVDTNGDGTLTFDEF----EELYKSLTERPELEPIFKKYaGTNRDYMTLEEFIRFLREEQGENVSEEECEELIEKY 115

                         90
                 ....*....|....
gi 546231697 104 DTY-YRGFLTLEDF 116
Cdd:cd15898  116 EPErENRQLSFEGF 129
EFh_PEF_Group_I cd16180
Penta-EF hand, calcium binding motifs, found in Group I PEF proteins; The family corresponds ...
 21-116 2.58e-03

Penta-EF hand, calcium binding motifs, found in Group I PEF proteins; The family corresponds to Group I PEF proteins that have been found not only in higher animals but also in lower animals, plants, fungi and protists. Group I PEF proteins include apoptosis-linked gene 2 protein (ALG-2), peflin and similar proteins. ALG-2, also termed programmed cell death protein 6 (PDCD6), is a widely expressed calcium-binding modulator protein associated with cell proliferation and death, as well as cell survival. It forms a homodimer in the cell or a heterodimer with its closest paralog peflin. Among the PEF proteins, ALG-2 can bind three Ca2+ ions through its EF1, EF3, and EF5 hands, where it is unique in that its EF5 hand binds Ca2+ ion in a canonical coordination. Peflin is a ubiquitously expressed 30-kD PEF protein containing five EF-hand motifs in its C-terminal domain and a longer N-terminal hydrophobic domain (NHB domain) than any other member of the PEF family. The NHB domain harbors nine repeats of a nonapeptide (A/PPGGPYGGP). Peflin may modulate the function of ALG-2 in Ca2+ signaling. It exists only as a heterodimer with ALG-2, and binds two Ca2+ ions through its EF1 and EF3 hands. Its additional EF5 hand is unpaired and does not bind Ca2+ ion but mediates the heterodimerization with ALG-2. The dissociation of heterodimer occurs in the presence of Ca2+.


Pssm-ID: 320055 [Multi-domain]  Cd Length: 164  Bit Score: 35.97  E-value: 2.58e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231697  21 RKWVEVFKACDEDHKGYLSREDFKTAVVMlFGYKPSKIEVDSVMSSINPNTSGILleGFLNIVRKKKEAQRYRNevrhIF 100
Cdd:cd16180   67 QDWRRLFRRFDRDRSGSIDFNELQNALSS-FGYRLSPQFVQLLVRKFDRRRRGSI--SFDDFVEACVTLKRLTD----AF 139

                         90
                 ....*....|....*...
gi 546231697 101 TAFDTYYRGFLTL--EDF 116
Cdd:cd16180  140 RKYDTNRTGYATIsyEDF 157
EFh_ScPlc1p_like cd16207
EF-hand motif found in Saccharomyces cerevisiae phospholipase C-1 (ScPlc1p) and similar ...
 25-116 3.43e-03

EF-hand motif found in Saccharomyces cerevisiae phospholipase C-1 (ScPlc1p) and similar proteins; This family represents a group of putative phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) encoded by PLC1 genes from yeasts, which are homologs of the delta isoforms of mammalian PI-PLC in terms of overall sequence similarity and domain organization. Mammalian PI-PLC is a signaling enzyme that hydrolyzes the membrane phospholipids phosphatidylinositol-4,5-bisphosphate (PIP2) to generate two important second messengers in eukaryotic signal transduction cascades, inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol (DAG). InsP3 triggers inflow of calcium from intracellular stores, while DAG, together with calcium, activates protein kinase C, which then phosphorylates other molecules, leading to altered cellular activity. Calcium is required for the catalysis. The prototype of this family is protein Plc1p (also termed 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1) encoded by PLC1 genes from Saccharomyces cerevisiae. ScPlc1p contains both highly conserved X- and Y- regions of PLC catalytic core domain, as well as a presumptive EF-hand like calcium binding motif. Experiments show that ScPlc1p displays calcium dependent catalytic properties with high similarity to those of the mammalian PLCs, and plays multiple roles in modulating the membrane/protein interactions in filamentation control. CaPlc1p encoded by CAPLC1 from the closely related yeast Candida albicans, an orthologue of S. cerevisiae Plc1p, is also included in this group. Like SCPlc1p, CaPlc1p has conserved presumptive catalytic domain, shows PLC activity when expressed in E. coli, and is involved in multiple cellular processes. There are two other gene copies of CAPLC1 in C. albicans, CAPLC2 (also named as PIPLC) and CAPLC3. Experiments show CaPlc1p is the only enzyme in C. albicans which functions as PLC. The biological functions of CAPLC2 and CAPLC3 gene products must be clearly different from CaPlc1p, but their exact roles remain unclear. Moreover, CAPLC2 and CAPLC3 gene products are more similar to extracellular bacterial PI-PLC than to the eukaryotic PI-PLC, and they are not included in this subfamily.


Pssm-ID: 320037 [Multi-domain]  Cd Length: 142  Bit Score: 35.30  E-value: 3.43e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231697  25 EVFKACDEDHKGYLSREDFKTaVVMLFGYKPskiEVDSVMSSI-NPNTSGILLEGFLNIVRKKKEAQRYRNEVRHIFTAF 103
Cdd:cd16207   42 ELFDKADTDKKGYLNFEEFQE-FVKLLKRRK---DIKAIFKQLtKPGSDGLTLEEFLKFLRDVQKEDVDRETWEKIFEKF 117

                         90
                 ....*....|....*..
gi 546231697 104 ----DTYYRGFLTLEDF 116
Cdd:cd16207  118 arriDDSDSLTMTLEGF 134
EFh_PEF_CAPN11 cd16193
Penta-EF hand, calcium binding motifs, found in calpain-11 (CAPN11); CAPN11, also termed ...
 21-47 6.69e-03

Penta-EF hand, calcium binding motifs, found in calpain-11 (CAPN11); CAPN11, also termed calcium-activated neutral proteinase 11 (CANP 11), is a mammalian orthologue of micro/m calpain. It is one of the calpain large subunits that appears to be exclusively expressed in certain cells of the testis. It may be involved in regulating calcium-dependent signal transduction events during meiosis and sperm functional processes. CAPN11 contains a short N-terminal anchor helix, followed by a calpain cysteine protease (CysPc) domain, a C2-domain-like (C2L) domain, and a C-terminal Ca2+-binding penta-EF-hand (PEF) domain.


Pssm-ID: 320068 [Multi-domain]  Cd Length: 169  Bit Score: 34.89  E-value: 6.69e-03
                         10        20
                 ....*....|....*....|....*..
gi 546231697  21 RKWVEVFKACDEDHKGYLSREDFKTAV 47
Cdd:cd16193   73 KKWMEIFKECDQDRSGNLNSYEMRLAI 99
PTZ00184 PTZ00184
calmodulin; Provisional
 25-137 7.07e-03

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 34.74  E-value: 7.07e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231697  25 EVFKACDEDHKGYLSREDFKTaVVMLFGYKPSKIEVDSVMSSINPNTSG-ILLEGFLNIVRKKKEAQRYRNEVRHIFTAF 103
Cdd:PTZ00184  15 EAFSLFDKDGDGTITTKELGT-VMRSLGQNPTEAELQDMINEVDADGNGtIDFPEFLTLMARKMKDTDSEEEIKEAFKVF 93

                         90       100       110
                 ....*....|....*....|....*....|....
gi 546231697 104 DTYYRGFLTLEDFKKAFRQVAPKLPERTVLEVFR 137
Cdd:PTZ00184  94 DRDGNGFISAAELRHVMTNLGEKLTDEEVDEMIR 127
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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