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Conserved domains on  [gi|546231866|ref|NP_001271197|]
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EF-hand calcium-binding domain-containing protein 11 isoform 4 [Homo sapiens]

Protein Classification

EF-hand domain-containing protein( domain architecture ID 12144783)

EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

CATH:  1.10.238.10
Gene Ontology:  GO:0005509
PubMed:  2479149|10191494
SCOP:  3001983

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
1-60 2.53e-05

EF-hand domain pair;


:

Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 39.16  E-value: 2.53e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 546231866    1 MVFKACDEDHKGYLSREDFKTAVVMLF-GYKPSKIEVDSVMSSINPNTSG-ILLEGFLNIVR 60
Cdd:pfam13499   6 EAFKLLDSDGDGYLDVEELKKLLRKLEeGEPLSDEEVEELFKEFDLDKDGrISFEEFLELYS 67
 
Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
1-60 2.53e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 39.16  E-value: 2.53e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 546231866    1 MVFKACDEDHKGYLSREDFKTAVVMLF-GYKPSKIEVDSVMSSINPNTSG-ILLEGFLNIVR 60
Cdd:pfam13499   6 EAFKLLDSDGDGYLDVEELKKLLRKLEeGEPLSDEEVEELFKEFDLDKDGrISFEEFLELYS 67
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
1-115 4.34e-05

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 40.16  E-value: 4.34e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231866   1 MVFKACDEDHKGYLSREDFKTAVVMLFGYKPSKIEVDSvmssinpnTSGILLEGFLNIVRKKKEAQRyRNEVRHIFTAFD 80
Cdd:COG5126    9 RRFDLLDADGDGVLERDDFEALFRRLWATLFSEADTDG--------DGRISREEFVAGMESLFEATV-EPFARAAFDLLD 79

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 546231866  81 TYYRGFLTLEDFKKAFRqvAPKLPERTVLEVFRGI 115
Cdd:COG5126   80 TDGDGKISADEFRRLLT--ALGVSEEEADELFARL 112
PTZ00183 PTZ00183
centrin; Provisional
 3-105 5.81e-04

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 37.36  E-value: 5.81e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231866   3 FKACDEDHKGYLSREDFKTAVVMLfGYKPSKIEVDSVMSSINPNTSGIL-LEGFLNIVRKKKEAQRYRNEVRHIFTAFDT 81
Cdd:PTZ00183  23 FDLFDTDGSGTIDPKELKVAMRSL-GFEPKKEEIKQMIADVDKDGSGKIdFEEFLDIMTKKLGERDPREEILKAFRLFDD 101

                         90       100
                 ....*....|....*....|....
gi 546231866  82 YYRGFLTLedfkKAFRQVAPKLPE 105
Cdd:PTZ00183 102 DKTGKISL----KNLKRVAKELGE 121
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 2-60 1.16e-03

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 34.83  E-value: 1.16e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231866   2 VFKACDEDHKGYLSREDFKTAVVMLfGYKPSKIEVDSVMSSINPNTSGIL-LEGFLNIVR 60
Cdd:cd00051    5 AFRLFDKDGDGTISADELKAALKSL-GEGLSEEEIDEMIREVDKDGDGKIdFEEFLELMA 63
 
Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
1-60 2.53e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 39.16  E-value: 2.53e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 546231866    1 MVFKACDEDHKGYLSREDFKTAVVMLF-GYKPSKIEVDSVMSSINPNTSG-ILLEGFLNIVR 60
Cdd:pfam13499   6 EAFKLLDSDGDGYLDVEELKKLLRKLEeGEPLSDEEVEELFKEFDLDKDGrISFEEFLELYS 67
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
1-115 4.34e-05

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 40.16  E-value: 4.34e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231866   1 MVFKACDEDHKGYLSREDFKTAVVMLFGYKPSKIEVDSvmssinpnTSGILLEGFLNIVRKKKEAQRyRNEVRHIFTAFD 80
Cdd:COG5126    9 RRFDLLDADGDGVLERDDFEALFRRLWATLFSEADTDG--------DGRISREEFVAGMESLFEATV-EPFARAAFDLLD 79

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 546231866  81 TYYRGFLTLEDFKKAFRqvAPKLPERTVLEVFRGI 115
Cdd:COG5126   80 TDGDGKISADEFRRLLT--ALGVSEEEADELFARL 112
PTZ00183 PTZ00183
centrin; Provisional
 3-105 5.81e-04

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 37.36  E-value: 5.81e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231866   3 FKACDEDHKGYLSREDFKTAVVMLfGYKPSKIEVDSVMSSINPNTSGIL-LEGFLNIVRKKKEAQRYRNEVRHIFTAFDT 81
Cdd:PTZ00183  23 FDLFDTDGSGTIDPKELKVAMRSL-GFEPKKEEIKQMIADVDKDGSGKIdFEEFLDIMTKKLGERDPREEILKAFRLFDD 101

                         90       100
                 ....*....|....*....|....
gi 546231866  82 YYRGFLTLedfkKAFRQVAPKLPE 105
Cdd:PTZ00183 102 DKTGKISL----KNLKRVAKELGE 121
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 2-60 1.16e-03

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 34.83  E-value: 1.16e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231866   2 VFKACDEDHKGYLSREDFKTAVVMLfGYKPSKIEVDSVMSSINPNTSGIL-LEGFLNIVR 60
Cdd:cd00051    5 AFRLFDKDGDGTISADELKAALKSL-GEGLSEEEIDEMIREVDKDGDGKIdFEEFLELMA 63
EFh_PI-PLC cd15898
EF-hand motif found in eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4. ...
 2-92 1.48e-03

EF-hand motif found in eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) isozymes; PI-PLC isozymes are signaling enzymes that hydrolyze the membrane phospholipids phosphatidylinositol-4,5-bisphosphate (PIP2) to generate two important second messengers in eukaryotic signal transduction cascades, Inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol (DAG). InsP3 triggers inflow of calcium from intracellular stores, while DAG, together with calcium, activates protein kinase C, which goes on to phosphorylate other molecules, leading to altered cellular activity. Calcium is required for the catalysis. This family corresponds to the four EF-hand motifs containing PI-PLC isozymes, including PI-PLC-beta (1-4), -gamma (1-2), -delta (1,3,4), -epsilon (1), -zeta (1), eta (1-2). Lower eukaryotes such as yeast and slime molds contain only delta-type isozymes. In contrast, other types of isoforms present in higher eukaryotes. This family also includes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (PLC1) from fungi. Some homologs from plants contain only two atypical EF-hand motifs and they are not included. All PI-PLC isozymes except sperm-specific PI-PLC-zeta share a core set of domains, including an N-terminal pleckstrin homology (PH) domain, four atypical EF-hand motifs, a PLC catalytic core, and a single C2 domain. PI-PLC-zeta lacks the PH domain. The PLC catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a highly degenerate linker sequence. Most of EF-hand motifs found in PI-PLCs consist of a helix-loop-helix structure, but lack residues critical to metal binding. Moreover, the EF-hand region of most of PI-PLCs may have an important regulatory function, but it has yet to be identified. However, PI-PLC-zeta is a key exception. It is responsible for Ca2+ oscillations in fertilized oocytes and exhibits a high sensitivity to Ca2+ mediated through its EF-hand domain. In addition, PI-PLC-eta2 shows a canonical EF-loop directing Ca2+-sensitivity and thus can amplify transient Ca2+ signals. Also it appears that PI-PLC-delta1 can regulate the binding of PH domain to PIP2 in a Ca2+-dependent manner through its functionally important EF-hand domains. PI-PLCs can be activated by a variety of extracellular ligands, such as growth factors, hormones, cytokines and lipids. Their activation has been implicated in tumorigenesis and/or metastasis linked to migration, proliferation, growth, inflammation, angiogenesis and actin cytoskeleton reorganization. PI-PLC-beta isozymes are activated by G-protein coupled receptor (GPCR) through different mechanisms. However, PI-PLC-gamma isozymes are activated by receptor tyrosine kinase (RTK), such as Rho and Ras GTPases. In contrast, PI-PLC-epsilon are activated by both GPCR and RTK. PI-PLC-delta1 and PLC-eta 1 are activated by GPCR-mediated calcium mobilization. The activation mechanism for PI-PLC-zeta remains unclear.


Pssm-ID: 320029 [Multi-domain]  Cd Length: 137  Bit Score: 35.72  E-value: 1.48e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231866   2 VFKACDEDHKGYLSREDFktavVMLFGYKPSKIEVDSVMSSI-NPNTSGILLEGFLNIVRKKKEAQRYRNEVRHIFTAFD 80
Cdd:cd15898   41 LFKEVDTNGDGTLTFDEF----EELYKSLTERPELEPIFKKYaGTNRDYMTLEEFIRFLREEQGENVSEEECEELIEKYE 116

                         90
                 ....*....|...
gi 546231866  81 TY-YRGFLTLEDF 92
Cdd:cd15898  117 PErENRQLSFEGF 129
EFh_ScPlc1p_like cd16207
EF-hand motif found in Saccharomyces cerevisiae phospholipase C-1 (ScPlc1p) and similar ...
 3-92 2.64e-03

EF-hand motif found in Saccharomyces cerevisiae phospholipase C-1 (ScPlc1p) and similar proteins; This family represents a group of putative phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) encoded by PLC1 genes from yeasts, which are homologs of the delta isoforms of mammalian PI-PLC in terms of overall sequence similarity and domain organization. Mammalian PI-PLC is a signaling enzyme that hydrolyzes the membrane phospholipids phosphatidylinositol-4,5-bisphosphate (PIP2) to generate two important second messengers in eukaryotic signal transduction cascades, inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol (DAG). InsP3 triggers inflow of calcium from intracellular stores, while DAG, together with calcium, activates protein kinase C, which then phosphorylates other molecules, leading to altered cellular activity. Calcium is required for the catalysis. The prototype of this family is protein Plc1p (also termed 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1) encoded by PLC1 genes from Saccharomyces cerevisiae. ScPlc1p contains both highly conserved X- and Y- regions of PLC catalytic core domain, as well as a presumptive EF-hand like calcium binding motif. Experiments show that ScPlc1p displays calcium dependent catalytic properties with high similarity to those of the mammalian PLCs, and plays multiple roles in modulating the membrane/protein interactions in filamentation control. CaPlc1p encoded by CAPLC1 from the closely related yeast Candida albicans, an orthologue of S. cerevisiae Plc1p, is also included in this group. Like SCPlc1p, CaPlc1p has conserved presumptive catalytic domain, shows PLC activity when expressed in E. coli, and is involved in multiple cellular processes. There are two other gene copies of CAPLC1 in C. albicans, CAPLC2 (also named as PIPLC) and CAPLC3. Experiments show CaPlc1p is the only enzyme in C. albicans which functions as PLC. The biological functions of CAPLC2 and CAPLC3 gene products must be clearly different from CaPlc1p, but their exact roles remain unclear. Moreover, CAPLC2 and CAPLC3 gene products are more similar to extracellular bacterial PI-PLC than to the eukaryotic PI-PLC, and they are not included in this subfamily.


Pssm-ID: 320037 [Multi-domain]  Cd Length: 142  Bit Score: 35.30  E-value: 2.64e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 546231866   3 FKACDEDHKGYLSREDFKTaVVMLFGYKPskiEVDSVMSSI-NPNTSGILLEGFLNIVRKKKEAQRYRNEVRHIFTAF-- 79
Cdd:cd16207   44 FDKADTDKKGYLNFEEFQE-FVKLLKRRK---DIKAIFKQLtKPGSDGLTLEEFLKFLRDVQKEDVDRETWEKIFEKFar 119

                         90
                 ....*....|....*
gi 546231866  80 --DTYYRGFLTLEDF 92
Cdd:cd16207  120 riDDSDSLTMTLEGF 134
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
1-61 3.51e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 34.77  E-value: 3.51e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 546231866   1 MVFKACDEDHKGYLSREDFKTavvMLFGYKPSKIEVDSVMSSINPNTSGIL-LEGFLNIVRK 61
Cdd:COG5126   73 AAFDLLDTDGDGKISADEFRR---LLTALGVSEEEADELFARLDTDGDGKIsFEEFVAAVRD 131
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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