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Conserved domains on  [gi|654823808|ref|NP_001280604|]
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carbonic anhydrase 2 isoform 2 [Homo sapiens]

Protein Classification

carbonic anhydrase family protein( domain architecture ID 275)

carbonic anhydrase family protein similar to carbonic anhydrase, which catalyzes the reversible hydration of gaseous carbon dioxide to carbonic acid

CATH:  3.10.200.10
Gene Ontology:  GO:0004089|GO:0008270|GO:0006730
PubMed:  10978542|18336305
SCOP:  4002732

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
alpha_CA super family cl00012
Carbonic anhydrase alpha (vertebrate-like) group. Carbonic anhydrases (CAs) are ...
 16-158 1.57e-104

Carbonic anhydrase alpha (vertebrate-like) group. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three evolutionary distinct groups - alpha, beta and gamma carbonic anhydrases - which show no significant sequence identity or structural similarity. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidine residues and a fourth conserved histidine plays a potential role in proton transfer.


The actual alignment was detected with superfamily member cd03119:

Pssm-ID: 469577 [Multi-domain]  Cd Length: 259  Bit Score: 299.35  E-value: 1.57e-104
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGS 95
Cdd:cd03119  117 ELHLVHWNSKYGSFGEAAKQPDGLAVVGVFLKVGEANPELQKVLDALDSIKTKGKQAPFTNFDPSCLLPASLDYWTYPGS 196

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 654823808  96 LTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASF 158
Cdd:cd03119  197 LTTPPLLECVTWIVLKEPISVSSEQMAKFRSLLFNAEGEPPCPMVDNWRPPQPLKGRKVRASF 259
 
Name Accession Description Interval E-value
alpha_CA_I_II_III_XIII cd03119
Carbonic anhydrase alpha, isozymes I, II, and III and XIII. Carbonic anhydrases (CAs) are ...
 16-158 1.57e-104

Carbonic anhydrase alpha, isozymes I, II, and III and XIII. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidines. This vertebrate subgroup comprises isozymes I, II, and III, which are cytoplasmic enzymes. CA I, for example, is expressed in erythrocyes of many vertebrates; CA II is the most active cytosolic isozyme; while it is being expressed nearly ubiquitously, it comprises 95% of the renal carbonic anhydrase and is required for renal acidification; CA III has been implicated in protection from the damaging effect of oxidizing agents in hepatocytes. CAXIII may play important physiological roles in several organs.


Pssm-ID: 239393 [Multi-domain]  Cd Length: 259  Bit Score: 299.35  E-value: 1.57e-104
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGS 95
Cdd:cd03119  117 ELHLVHWNSKYGSFGEAAKQPDGLAVVGVFLKVGEANPELQKVLDALDSIKTKGKQAPFTNFDPSCLLPASLDYWTYPGS 196

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 654823808  96 LTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASF 158
Cdd:cd03119  197 LTTPPLLECVTWIVLKEPISVSSEQMAKFRSLLFNAEGEPPCPMVDNWRPPQPLKGRKVRASF 259
Carb_anhydrase pfam00194
Eukaryotic-type carbonic anhydrase;
17-158 8.53e-76

Eukaryotic-type carbonic anhydrase;


Pssm-ID: 459707 [Multi-domain]  Cd Length: 252  Bit Score: 226.38  E-value: 8.53e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808   17 LHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAK-PGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESL-DYWTYPG 94
Cdd:pfam00194 109 LHIVHYNSKYKSFDEAAKHPDGLAVLGVFFEVGDENnPYLQPIVSALDNIKYKGKSVLLPPFDLSDLLPEDLtSYYTYNG 188

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 654823808   95 SLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASF 158
Cdd:pfam00194 189 SLTTPPCSESVTWIVFKEPISISEEQLEAFRTLLFSDGGEEPRPLVNNFRPTQPLNGRVVFASF 252
Carb_anhydrase smart01057
Eukaryotic-type carbonic anhydrase; Carbonic anhydrases are zinc metalloenzymes which catalyse ...
 17-153 1.45e-63

Eukaryotic-type carbonic anhydrase; Carbonic anhydrases are zinc metalloenzymes which catalyse the reversible hydration of carbon dioxide to bicarbonate.. CAs have essential roles in facilitating the transport of carbon dioxide and protons in the intracellular space, across biological membranes and in the layers of the extracellular space; they are also involved in many other processes, from respiration and photosynthesis in eukaryotes to cyanate degradation in prokaryotes. There are five known evolutionarily distinct CA families (alpha, beta, gamma, delta and epsilon) that have no significant sequence identity and have structurally distinct overall folds. Some CAs are membrane-bound, while others act in the cytosol; there are several related proteins that lack enzymatic activity. The active site of alpha-CAs is well described, consisting of a zinc ion coordinated through 3 histidine residues and a water molecule/hydroxide ion that acts as a potent nucleophile. The enzyme employs a two-step mechanism: in the first step, there is a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide; in the second step, the active site is regenerated by the ionisation of the zinc-bound water molecule and the removal of a proton from the active site. Beta- and gamma-CAs also employ a zinc hydroxide mechanism, although at least some beta-class enzymes do not have water directly coordinated to the metal ion.


Pssm-ID: 215000 [Multi-domain]  Cd Length: 247  Bit Score: 195.23  E-value: 1.45e-63
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808    17 LHLVHWNTKyGDFGKAVQQPDGLAVLGIFLKVGSAK-PGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESL-DYWTYPG 94
Cdd:smart01057 113 LHLVHYNSK-GSFSEAVSKPGGLAVVAVFFKVGAEEnPALQAILDHLPLIKYKGQETELTPFDLSSLLPASTrHYYTYNG 191

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 654823808    95 SLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPeelMVDNWRPAQPLKNRQ 153
Cdd:smart01057 192 SLTTPPCSEGVTWIVFKEPITISTEQLEKFRTLLPMEGNEP---LVNNARPLQPLNGRV 247
Cah COG3338
Carbonic anhydrase [Inorganic ion transport and metabolism];
17-157 9.95e-40

Carbonic anhydrase [Inorganic ion transport and metabolism];


Pssm-ID: 442567 [Multi-domain]  Cd Length: 247  Bit Score: 134.24  E-value: 9.95e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  17 LHLVHwntkygdfgkavQQPDG-LAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTnFDPRGLLPESLDYWTYPGS 95
Cdd:COG3338  130 AHLVH------------KDADGeLAVVGVLFEEGAENPALAKLWANLPLEAGEEVALDAT-IDLNDLLPEDRSYYRYSGS 196

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 654823808  96 LTTPPLLECVTWIVLKEPISVSSEQVLKFRKlnfngegepeeLMVDNWRPAQPLKNRQIKAS 157
Cdd:COG3338  197 LTTPPCSEGVLWIVLKQPITVSAEQIEAFAR-----------LYPNNARPVQPLNGRLILES 247
PLN02202 PLN02202
carbonate dehydratase
 16-155 5.21e-11

carbonate dehydratase


Pssm-ID: 177853 [Multi-domain]  Cd Length: 284  Bit Score: 59.30  E-value: 5.21e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHwntkygdfgkavQQPDG-LAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFT------NFDPRGLLPESLD 88
Cdd:PLN02202 136 ELHMVH------------QAKDGsFAVVASLFKIGTEEPFLSQMKDKLVKLKEERFKGNHTaqvevgKIDTRHIERKTRK 203

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 654823808  89 YWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKlnfngegEPEELMVDNWRPAQPLKNRQIK 155
Cdd:PLN02202 204 YFRYIGSLTTPPCSENVSWTILGKVRSMSKEQVELLRS-------PLDKSFKNNSRPCQPLNGRRVE 263
 
Name Accession Description Interval E-value
alpha_CA_I_II_III_XIII cd03119
Carbonic anhydrase alpha, isozymes I, II, and III and XIII. Carbonic anhydrases (CAs) are ...
 16-158 1.57e-104

Carbonic anhydrase alpha, isozymes I, II, and III and XIII. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidines. This vertebrate subgroup comprises isozymes I, II, and III, which are cytoplasmic enzymes. CA I, for example, is expressed in erythrocyes of many vertebrates; CA II is the most active cytosolic isozyme; while it is being expressed nearly ubiquitously, it comprises 95% of the renal carbonic anhydrase and is required for renal acidification; CA III has been implicated in protection from the damaging effect of oxidizing agents in hepatocytes. CAXIII may play important physiological roles in several organs.


Pssm-ID: 239393 [Multi-domain]  Cd Length: 259  Bit Score: 299.35  E-value: 1.57e-104
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGS 95
Cdd:cd03119  117 ELHLVHWNSKYGSFGEAAKQPDGLAVVGVFLKVGEANPELQKVLDALDSIKTKGKQAPFTNFDPSCLLPASLDYWTYPGS 196

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 654823808  96 LTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASF 158
Cdd:cd03119  197 LTTPPLLECVTWIVLKEPISVSSEQMAKFRSLLFNAEGEPPCPMVDNWRPPQPLKGRKVRASF 259
Carb_anhydrase pfam00194
Eukaryotic-type carbonic anhydrase;
17-158 8.53e-76

Eukaryotic-type carbonic anhydrase;


Pssm-ID: 459707 [Multi-domain]  Cd Length: 252  Bit Score: 226.38  E-value: 8.53e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808   17 LHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAK-PGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESL-DYWTYPG 94
Cdd:pfam00194 109 LHIVHYNSKYKSFDEAAKHPDGLAVLGVFFEVGDENnPYLQPIVSALDNIKYKGKSVLLPPFDLSDLLPEDLtSYYTYNG 188

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 654823808   95 SLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASF 158
Cdd:pfam00194 189 SLTTPPCSESVTWIVFKEPISISEEQLEAFRTLLFSDGGEEPRPLVNNFRPTQPLNGRVVFASF 252
alpha_CA_VII cd03149
Carbonic anhydrase alpha, CA isozyme VII_like subgroup. Carbonic anhydrases (CAs) are ...
 16-158 9.48e-72

Carbonic anhydrase alpha, CA isozyme VII_like subgroup. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidines. This vertebrate subgroup comprises isozyme VII. CA VII is the most active cytosolic enzyme after CA II, and may be highly expressed in the brain. Human CA VII may be a target of antiepileptic sulfonamides/sulfamates.


Pssm-ID: 239402  Cd Length: 236  Bit Score: 215.47  E-value: 9.48e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNT-KYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPG 94
Cdd:cd03149   93 ELHLVHWNAkKYKSFGEAAAAPDGLAVLGVFLETGDEHPGLNRLTDALYMVRFKGTKAQFLDFNPKCLLPKSLDYWTYPG 172

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 654823808  95 SLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASF 158
Cdd:cd03149  173 SLTTPPLNESVTWIVLKEPIPVSEKQMGKFRELLFTSEEDQRNHMVNNFRPPQPLKGRTVRASF 236
alpha_CA_V cd03118
Carbonic anhydrase alpha, CA isozyme V_like subgroup. Carbonic anhydrases (CAs) are ...
 16-158 2.32e-66

Carbonic anhydrase alpha, CA isozyme V_like subgroup. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidines. This vertebrate subgroup comprises isozyme V. CA V is the mitochondrial isozyme, which may play a role in gluconeogenesis and ureagenesis and possibly also in lipogenesis.


Pssm-ID: 239392  Cd Length: 236  Bit Score: 202.00  E-value: 2.32e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWN-TKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPG 94
Cdd:cd03118   93 ELHLVHWNsVKYENFEEAVMEENGLAVIGVFLKLGAHHEGLQKLVDALPEVRHKDTVVEFNPFDPSCLLPACRDYWTYPG 172

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 654823808  95 SLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASF 158
Cdd:cd03118  173 SLTTPPLTESVTWIIQKQPIEVSPSQLSVFRTLLFTSRGEEEKVMVNNFRPLQPLMNRKVRSSF 236
Carb_anhydrase smart01057
Eukaryotic-type carbonic anhydrase; Carbonic anhydrases are zinc metalloenzymes which catalyse ...
 17-153 1.45e-63

Eukaryotic-type carbonic anhydrase; Carbonic anhydrases are zinc metalloenzymes which catalyse the reversible hydration of carbon dioxide to bicarbonate.. CAs have essential roles in facilitating the transport of carbon dioxide and protons in the intracellular space, across biological membranes and in the layers of the extracellular space; they are also involved in many other processes, from respiration and photosynthesis in eukaryotes to cyanate degradation in prokaryotes. There are five known evolutionarily distinct CA families (alpha, beta, gamma, delta and epsilon) that have no significant sequence identity and have structurally distinct overall folds. Some CAs are membrane-bound, while others act in the cytosol; there are several related proteins that lack enzymatic activity. The active site of alpha-CAs is well described, consisting of a zinc ion coordinated through 3 histidine residues and a water molecule/hydroxide ion that acts as a potent nucleophile. The enzyme employs a two-step mechanism: in the first step, there is a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide; in the second step, the active site is regenerated by the ionisation of the zinc-bound water molecule and the removal of a proton from the active site. Beta- and gamma-CAs also employ a zinc hydroxide mechanism, although at least some beta-class enzymes do not have water directly coordinated to the metal ion.


Pssm-ID: 215000 [Multi-domain]  Cd Length: 247  Bit Score: 195.23  E-value: 1.45e-63
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808    17 LHLVHWNTKyGDFGKAVQQPDGLAVLGIFLKVGSAK-PGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESL-DYWTYPG 94
Cdd:smart01057 113 LHLVHYNSK-GSFSEAVSKPGGLAVVAVFFKVGAEEnPALQAILDHLPLIKYKGQETELTPFDLSSLLPASTrHYYTYNG 191

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 654823808    95 SLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPeelMVDNWRPAQPLKNRQ 153
Cdd:smart01057 192 SLTTPPCSEGVTWIVFKEPITISTEQLEKFRTLLPMEGNEP---LVNNARPLQPLNGRV 247
alpha_CA cd00326
Carbonic anhydrase alpha (vertebrate-like) group. Carbonic anhydrases (CAs) are ...
 17-154 5.76e-61

Carbonic anhydrase alpha (vertebrate-like) group. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three evolutionary distinct groups - alpha, beta and gamma carbonic anhydrases - which show no significant sequence identity or structural similarity. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidine residues and a fourth conserved histidine plays a potential role in proton transfer.


Pssm-ID: 238200  Cd Length: 227  Bit Score: 187.88  E-value: 5.76e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  17 LHLVHWNTKYGDFgKAVQQPDGLAVLGIFLKVGSAK-PGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESL-DYWTYPG 94
Cdd:cd00326   92 LHLVHYNSDYYSS-EAAKKPGGLAVLGVFFEVGEKEnPFLKKILDALPKIKYKGKETTLPPFDLSDLLPSSLrDYYTYEG 170

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  95 SLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNgegePEELMVDNWRPAQPLKNRQI 154
Cdd:cd00326  171 SLTTPPCSEGVTWIVFKEPITISKEQLEAFRSLLDR----EGKPLVNNYRPVQPLNGRVV 226
alpha_CA_VI_IX_XII_XIV cd03123
Carbonic anhydrase alpha, isozymes VI, IX, XII and XIV. Carbonic anhydrases (CAs) are ...
 16-158 2.29e-41

Carbonic anhydrase alpha, isozymes VI, IX, XII and XIV. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three evolutionary distinct groups - alpha, beta and gamma carbonic anhydrases - which show no significant sequence identity or structural similarity. Alpha CAs are mostly monomeric enzymes. The zinc ion is complexed by three histidine residues. This sub-family comprises the secreted CA VI, which is found in saliva, for example, and the membrane proteins CA IX, XII, and XIV.


Pssm-ID: 239397 [Multi-domain]  Cd Length: 248  Bit Score: 138.59  E-value: 2.29e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNT-KYGDFGKAVQQPDGLAVLGIFLKVGSAK-PGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLD-YWTY 92
Cdd:cd03123  106 ELHIVHYNSdKYSSFDEAADKPDGLAVLAILIEVGYPEnTYYEKIISHLHEIKYKGQETTVPGFNVRELLPEDLShYYRY 185

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 654823808  93 PGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEgepEELMVDNWRPAQPLKNRQIKASF 158
Cdd:cd03123  186 EGSLTTPPCYESVLWTVFRDPVTLSKEQLETLENTLMDTH---NKTLQNNYRATQPLNGRVVEASF 248
alpha_CARP_VIII cd03120
Carbonic anhydrase alpha related protein, group VIII. Carbonic anhydrase related proteins ...
 16-158 3.96e-41

Carbonic anhydrase alpha related protein, group VIII. Carbonic anhydrase related proteins (CARPs) are sequence similar to carbonic anhydrases. Carbonic anhydrases are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism. CARPs have lost conserved histidines involved in zinc binding and consequently their catalytic activity. CARP VIII may play roles in various biological processes of the central nervous system, and could be involved in protein-protein interactions. CARP VIII has been shown to bind inositol 1,4,5-triphosphate (IP3) receptor type I (IP3RI), reducing the affinity of the receptor for IP3. IP3RI is an intracellular IP3-gated Ca2+ channel located on intracellular Ca2+ stores. IP3RI converts IP3 signaling into Ca2+ signaling thereby participating in a variety of cell functions.


Pssm-ID: 239394  Cd Length: 256  Bit Score: 138.07  E-value: 3.96e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWN-TKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESL--DYWTY 92
Cdd:cd03120  106 ELHLIHWNsTLYSSLEEAMGKPHGIAIIALFVQIGKEHVGLKAVTEILQDIQYKGKSKTIPCFNPNTLLPDPLlrDYWVY 185

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 654823808  93 PGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEP-----EELMVDNWRPAQPLKNRQIKASF 158
Cdd:cd03120  186 EGSLTTPPCSEGVTWILFRYPLTISQSQIEEFRRLRTHVKGAElvegcDGLLGDNFRPTQPLSDRVIRAAF 256
alpha_CA_IV_XV_like cd03117
Carbonic anhydrase alpha, CA_IV, CA_XV, like isozymes. Carbonic anhydrases (CAs) are ...
 17-156 5.52e-40

Carbonic anhydrase alpha, CA_IV, CA_XV, like isozymes. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three evolutionary distinct groups - alpha, beta and gamma carbonic anhydrases - which show no significant sequence identity or structural similarity. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidine residues. This subgroup, restricted to animals, contains isozyme IV and similar proteins such as mouse CA XV. Isozymes IV is attached to membranes via a glycosylphosphatidylinositol (GPI) tail. In mammals, Isozyme IV plays crucial roles in kidney and lung function, amongst others. This subgroup also contains the dual domain CA from the giant clam, Tridacna gigas. T. gigas CA plays a role in the movement of inorganic carbon from the surrounding seawater to the symbiotic algae found in the clam's tissues. CA XV is expressed in several species but not in humans or chimps. Similar to isozyme CA IV, CA XV attaches to membranes via a GPI tail.


Pssm-ID: 239391  Cd Length: 234  Bit Score: 134.32  E-value: 5.52e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  17 LHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKP-GLQKVVDVLDSIKTKGKSADFTNFDPRGLLP--ESLDYWTYP 93
Cdd:cd03117   93 LHIVHIKESYNSLLEALKDSDGLAVLGFFIEEGEEENtNFDPLISALSNIPQKGGSTNLTPFSLRSLLPsvLLTKYYRYN 172

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 654823808  94 GSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPeELMVDNWRPAQPLKNRQIKA 156
Cdd:cd03117  173 GSLTTPGCNEAVIWTVFEEPIPISRAQLDAFSTVLFFDTDNG-QPMVNNFRPVQPLNGRVVYA 234
alpha_CA_XII_XIV cd03126
Carbonic anhydrase alpha, isozymes XII and XIV. Carbonic anhydrases (CAs) are zinc-containing ...
 16-158 6.74e-40

Carbonic anhydrase alpha, isozymes XII and XIV. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three evolutionary distinct groups - alpha, beta and gamma carbonic anhydrases - which show no significant sequence identity or structural similarity. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidine residues. This sub-family comprises the membrane proteins CA XII and XIV.


Pssm-ID: 239400  Cd Length: 249  Bit Score: 134.96  E-value: 6.74e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNT-KYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLD-YWTYP 93
Cdd:cd03126  105 ELHIVHYNSdKYPDISTAMNKSQGLAVLGILIEVGPFNPSYEKIFSHLHEVKYKDQKVSVPGFNVQELLPKRLDeYYRYE 184

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 654823808  94 GSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASF 158
Cdd:cd03126  185 GSLTTPPCYPSVLWTVFRNPVQISQEQLLALETALYSTEEDESREMVNNYRQVQPFNERLVFASF 249
Cah COG3338
Carbonic anhydrase [Inorganic ion transport and metabolism];
17-157 9.95e-40

Carbonic anhydrase [Inorganic ion transport and metabolism];


Pssm-ID: 442567 [Multi-domain]  Cd Length: 247  Bit Score: 134.24  E-value: 9.95e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  17 LHLVHwntkygdfgkavQQPDG-LAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTnFDPRGLLPESLDYWTYPGS 95
Cdd:COG3338  130 AHLVH------------KDADGeLAVVGVLFEEGAENPALAKLWANLPLEAGEEVALDAT-IDLNDLLPEDRSYYRYSGS 196

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 654823808  96 LTTPPLLECVTWIVLKEPISVSSEQVLKFRKlnfngegepeeLMVDNWRPAQPLKNRQIKAS 157
Cdd:COG3338  197 LTTPPCSEGVLWIVLKQPITVSAEQIEAFAR-----------LYPNNARPVQPLNGRLILES 247
alpha_CA_prokaryotic_like cd03124
Carbonic anhydrase alpha, prokaryotic-like subfamily. Carbonic anhydrases (CAs) are ...
 17-154 5.83e-36

Carbonic anhydrase alpha, prokaryotic-like subfamily. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidines. This sub-family includes bacterial carbonic anhydrase alpha, as well as plant enzymes such as tobacco nectarin III and yam dioscorin and, carbonic anhydrases from molluscs, such as nacrein, which are part of the organic matrix layer in shells. Other members of this family may be involved in maintaining pH balance, in facilitating transport of carbon dioxide or carbonic acid, or in sensing carbon dioxide levels in the environment. Dioscorin is the major storage protein of yam tubers and may play a role as an antioxidant. Tobacco Nectarin may play a role in the maintenace of pH and oxidative balance in nectar. Mollusc nacrein may participate in calcium carbonate crystal formation of the nacreous layer. This subfamily also includes three alpha carbonic anhydrases from Chlamydomonas reinhardtii (CAH 1-3). CAHs1-2 are localized in the periplasmic space. CAH1 faciliates the movement of carbon dioxide across the plasma membrane when the medium is alkaline. CAH3 is localized to the thylakoid lumen and provides CO2 to Rubisco.


Pssm-ID: 239398 [Multi-domain]  Cd Length: 216  Bit Score: 123.54  E-value: 5.83e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  17 LHLVHwntkygdfgkavQQPDG-LAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKsADFTNFDPRGLLPESLDYWTYPGS 95
Cdd:cd03124   99 AHLVH------------KSKDGqLAVVAVLFEEGKENPFLKKILDNMPKKEGTEV-NLPAILDPNELLPESRSYYRYEGS 165

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 654823808  96 LTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGegepeelmvdNWRPAQPLKNRQI 154
Cdd:cd03124  166 LTTPPCSEGVRWIVLKQPITISKEQLAKFRAAVYPN----------NARPVQPLNGREV 214
alpha_CARP_X_XI_like cd03121
Carbonic anhydrase alpha related protein: groups X, XI and related proteins. This subgroup ...
 16-155 3.55e-32

Carbonic anhydrase alpha related protein: groups X, XI and related proteins. This subgroup contains carbonic anhydrase related proteins (CARPs) X and XI, which have been implicated in various biological processes of the central nervous system. CARPs are sequence similar to carbonic anhydrases. Carbonic anhydrases are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism. CARPs have lost conserved histidines involved in zinc binding and consequently their catalytic activity. CARP XI plays a role in the development of gastrointestinal stromal tumors.


Pssm-ID: 239395 [Multi-domain]  Cd Length: 256  Bit Score: 115.20  E-value: 3.55e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNTK-YGDFGKAVQQPDGLAVLGIFLKVGS-AKPGLQKVVDVLD--SIKTKGKSADFTNFDPRGLLPESLDYWT 91
Cdd:cd03121  109 EVQLIHYNSElYPNFSEASKSPNGLVIVSLFVKIGEtSNPELRRLTNRDTitSIRYKGDAYFLQDLSIELLLPETDHYIT 188

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 654823808  92 YPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIK 155
Cdd:cd03121  189 YEGSLTSPGCHETVTWIILNKPIYITKEQMHSLRLLSQNSPSQEKAPMSPNFRPVQPLNNRPVR 252
alpha_CA_VI cd03125
Carbonic anhydrase alpha, isozyme VI. Carbonic anhydrases (CAs) are zinc-containing enzymes ...
 16-158 6.25e-32

Carbonic anhydrase alpha, isozyme VI. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three evolutionary distinct groups - alpha, beta and gamma carbonic anhydrases - which show no significant sequence identity or structural similarity. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidine residues. This sub-family comprises the secreted CA VI, which is found in saliva.


Pssm-ID: 239399  Cd Length: 249  Bit Score: 114.11  E-value: 6.25e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPG--LQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLD-YWTY 92
Cdd:cd03125  106 ELHIVHYNSKYKSYEEAKDKPDGLAVLAFLYKVGHYAENtyYSDFISKLAKIKYAGQTTTLTSLDVRDMLPENLHhYYTY 185

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 654823808  93 PGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRK--LNFNgegepEELMVDNWRPAQPLKNRQIKASF 158
Cdd:cd03125  186 QGSLTTPPCTENVLWFVFDDPVTLSKTQIVKLENtlMDHH-----NKTIRNDYRRTQPLNHRVVEANF 248
alpha_CA_IX cd03150
Carbonic anhydrase alpha, isozyme IX. Carbonic anhydrases (CAs) are zinc-containing enzymes ...
 16-158 1.49e-29

Carbonic anhydrase alpha, isozyme IX. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three evolutionary distinct groups - alpha, beta and gamma carbonic anhydrases - which show no significant sequence identity or structural similarity. Alpha CAs are strictly monomeric enzymes. The zinc ion is complexed by three histidine residues. This sub-family comprises the membrane protein CA IX. CA IX is functionally implicated in tumor growth and survival. CA IX is mainly present in solid tumors and its expression in normal tissues is limited to the mucosa of alimentary tract. CA IX is a transmembrane protein with two extracellular domains: carbonic anhydrase and, a proteoglycan-like segment mediating cell-cell adhesion. There is evidence for an involvement of the MAPK pathway in the regulation of CA9 expression.


Pssm-ID: 239403  Cd Length: 247  Bit Score: 108.12  E-value: 1.49e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAK-PGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLD-YWTYP 93
Cdd:cd03150  105 EIHVVHLSTAFANLDEALGRPGGLAVLAAFLAEGLHEnSAYEQLLSRLSEISEEESETVVPGLDVSALLPSDLSrYFRYE 184

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 654823808  94 GSLTTPPLLECVTWIVLKEPISVSSEQVlkfRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASF 158
Cdd:cd03150  185 GSLTTPPCAQGVIWTVFNQTVRLSAKQL---HTLSDSLWGPHDSRLQLNFRATQPLNGRKIEASF 246
alpha_CARP_receptor_like cd03122
Carbonic anhydrase alpha related protein, receptor_like subfamily. Carbonic anhydrase related ...
 16-154 5.44e-28

Carbonic anhydrase alpha related protein, receptor_like subfamily. Carbonic anhydrase related proteins (CARPs) are sequence similar to carbonic anhydrases. Carbonic anhydrases are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism. CARPs have lost conserved histidines involved in zinc binding and consequently their catalytic activity. This sub-family of carbonic anhydrase-related domains found in tyrosine phosphatase receptors may play a role in cell adhesion.


Pssm-ID: 239396 [Multi-domain]  Cd Length: 253  Bit Score: 103.97  E-value: 5.44e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAK-PGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLD-YWTYP 93
Cdd:cd03122  109 EMQILHRNTDFFDSFEAIKSPGGVLALAYLFELSHEDnPFLDPIIEGLRNVSRPGKEVELPPFPLSDLLPPFTDkYYSYE 188

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 654823808  94 GSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLnFNGEGEPEELM---VDNWRPAQPLKNRQI 154
Cdd:cd03122  189 GSLTTPPCSETVEWIVFREPVPISSRQLEAFREL-LTRRQDGVMSGdylPNNGRPQQPLGSRTV 251
PLN02202 PLN02202
carbonate dehydratase
 16-155 5.21e-11

carbonate dehydratase


Pssm-ID: 177853 [Multi-domain]  Cd Length: 284  Bit Score: 59.30  E-value: 5.21e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHwntkygdfgkavQQPDG-LAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFT------NFDPRGLLPESLD 88
Cdd:PLN02202 136 ELHMVH------------QAKDGsFAVVASLFKIGTEEPFLSQMKDKLVKLKEERFKGNHTaqvevgKIDTRHIERKTRK 203

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 654823808  89 YWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKlnfngegEPEELMVDNWRPAQPLKNRQIK 155
Cdd:PLN02202 204 YFRYIGSLTTPPCSENVSWTILGKVRSMSKEQVELLRS-------PLDKSFKNNSRPCQPLNGRRVE 263
PLN02179 PLN02179
carbonic anhydrase
 16-111 1.13e-07

carbonic anhydrase


Pssm-ID: 177835  Cd Length: 235  Bit Score: 49.21  E-value: 1.13e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 654823808  16 QLHLVHWNTKygdfGKAvqqpdglAVLGIFLKVGSAKPGLQKVvdvLDSIKTKG-KSADFTNFDPRGLLPESLDYWTYPG 94
Cdd:PLN02179 143 ELHMVHTSAS----GKT-------AVVGVLYKLGEPDEFLTKL---LNGIKGVGkKEINLGIVDPRDIRFETNNFYRYIG 208

                         90
                 ....*....|....*..
gi 654823808  95 SLTTPPLLECVTWIVLK 111
Cdd:PLN02179 209 SLTIPPCTEGVIWTVVK 225
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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