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Conserved domains on  [gi|1016470456|ref|NP_001309266|]
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RWD domain-containing protein 2A isoform c [Homo sapiens]

Protein Classification

RWD and DUF1115 domain-containing protein( domain architecture ID 10250613)

RWD and DUF1115 domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RWDD2_C cd24163
C-terminal domain of RWD domain-containing protein 2; RWDD2A, as well as RWDD2B, are small ...
 89-218 5.38e-47

C-terminal domain of RWD domain-containing protein 2; RWDD2A, as well as RWDD2B, are small proteins that contain an N-terminal RWD domain and a C-terminal domain of unknown function. The C-terminal domain is predicted (by AlphaFold) to adopt a fold similar to the C-terminal domain of Prp3, an essential U4/U6 di-snRNP-associated protein which plays a role in pre-mRNA splicing. The Prp3 C-terminal domain is a single-stranded RNA-binding domain.


:

Pssm-ID: 467814  Cd Length: 122  Bit Score: 151.26  E-value: 5.38e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1016470456  89 FLRMWIYSHHIYQQDLRKKILDVGKRLDVTGFCMTGKPGIICVEGFKEHCEEFWHTIRYPNWKHISCKHAESVEtegnge 168
Cdd:cd24163     1 LGRRWIYSHHIYSKKKRKDIVEWAKELGLTGFSKPGKPGVICVEGDEEDVDEYVRRIRRLRWKKIQVRGEEVIE------ 74

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1016470456 169 dlrLFHSFEELLLEAHGDYGLRNDYHMNL-GQFLEFLKKHKSEHVFQILFG 218
Cdd:cd24163    75 ---VPRFFGEEEDEERALPGGFRELHEDLmGELAAFLEEAGLEELFLTLFG 122
RWD_DRWD_ELF-like super family cl45901
RWD, DRWD, and ELF domain family; The family includes the RWD, double-RWD (DRWD), and ELF ...
 1-69 9.31e-28

RWD, DRWD, and ELF domain family; The family includes the RWD, double-RWD (DRWD), and ELF domains. They belong to the ubiquitin-conjugating (UBC) superfamily that represents a structural domain with an alpha-beta(4)-alpha(3) core fold. The RWD domain (named after three major RWD-containing proteins: RING finger, WD-repeat-containing proteins and DEXD-like helicases) mediates protein-protein interactions in a variety of pathways in eukaryotes. The DRWD domain is responsible for substrate binding. It is involved in interactions with other kinetochore proteins. The ELF (N-terminal E2-like fold) domain is found in all Fanconi anemia group L protein (FANCL) homologs. It is required to promote efficient DNA damage-induced FANCD2 (Fanconi anemia group D2 protein) monoubiquitination in vertebrate cells.


The actual alignment was detected with superfamily member cd23829:

Pssm-ID: 480265  Cd Length: 129  Bit Score: 102.27  E-value: 9.31e-28
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1016470456   1 MVKIDLQVTMPHSYPYVALQLFGRSSELDRHQQLLLNKGLTSYIGTFDPGELCVCAAIQWLQDNSASYF 69
Cdd:cd23829    61 KVKVELSVTLPHEYPSVPPEIFVRSDSLSRSQQRQLNEDLSEYISSLERGELCILQIVQWLQDNASSYI 129
 
Name Accession Description Interval E-value
RWDD2_C cd24163
C-terminal domain of RWD domain-containing protein 2; RWDD2A, as well as RWDD2B, are small ...
 89-218 5.38e-47

C-terminal domain of RWD domain-containing protein 2; RWDD2A, as well as RWDD2B, are small proteins that contain an N-terminal RWD domain and a C-terminal domain of unknown function. The C-terminal domain is predicted (by AlphaFold) to adopt a fold similar to the C-terminal domain of Prp3, an essential U4/U6 di-snRNP-associated protein which plays a role in pre-mRNA splicing. The Prp3 C-terminal domain is a single-stranded RNA-binding domain.


Pssm-ID: 467814  Cd Length: 122  Bit Score: 151.26  E-value: 5.38e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1016470456  89 FLRMWIYSHHIYQQDLRKKILDVGKRLDVTGFCMTGKPGIICVEGFKEHCEEFWHTIRYPNWKHISCKHAESVEtegnge 168
Cdd:cd24163     1 LGRRWIYSHHIYSKKKRKDIVEWAKELGLTGFSKPGKPGVICVEGDEEDVDEYVRRIRRLRWKKIQVRGEEVIE------ 74

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1016470456 169 dlrLFHSFEELLLEAHGDYGLRNDYHMNL-GQFLEFLKKHKSEHVFQILFG 218
Cdd:cd24163    75 ---VPRFFGEEEDEERALPGGFRELHEDLmGELAAFLEEAGLEELFLTLFG 122
RWD_RWDD2 cd23829
RWD domain of RWD domain-containing protein 2A (RWDD2A), 2B (RWDD2B) and related proteins; ...
 1-69 9.31e-28

RWD domain of RWD domain-containing protein 2A (RWDD2A), 2B (RWDD2B) and related proteins; This subfamily includes RWDD2A, previously known as RWD domain-containing 2 (RWDD2) and RWDD2B, previously known in humans as chromosome 21 open reading frame (C21orf6). C21orf6 appears to be involved in monosomy 21 phenotype. The RWD domain may mediate protein-protein interactions.


Pssm-ID: 467663  Cd Length: 129  Bit Score: 102.27  E-value: 9.31e-28
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1016470456   1 MVKIDLQVTMPHSYPYVALQLFGRSSELDRHQQLLLNKGLTSYIGTFDPGELCVCAAIQWLQDNSASYF 69
Cdd:cd23829    61 KVKVELSVTLPHEYPSVPPEIFVRSDSLSRSQQRQLNEDLSEYISSLERGELCILQIVQWLQDNASSYI 129
DUF1115 pfam06544
Protein of unknown function (DUF1115); This family represents the C-terminus of hypothetical ...
 93-215 1.12e-13

Protein of unknown function (DUF1115); This family represents the C-terminus of hypothetical eukaryotic proteins of unknown function.


Pssm-ID: 461943  Cd Length: 135  Bit Score: 65.39  E-value: 1.12e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1016470456  93 WIYSHHIYQQDLRKKILDVGKRLDVTGFCMTGKP-GIICVEGFKEHCEEFWHTI-RYPNWKHISCK----HAESVETEGN 166
Cdd:pfam06544   2 VFRIKSLSNPKHRFKIDKNAKQLGLTGFCILGPPfGLVVVEGGEKSIKKYKKLMlRRIKWTENPHEdfeaMKEITFEWDN 81

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1016470456 167 GEDLRLFHSFEELL----LEAHGDYGLRNDYHMNLGQFLEFLKKHKSEHVFQI 215
Cdd:pfam06544  82 DEDNEGEDNKCELVwegeVKERAFRKWKFKVCETEGEAREFLKKHGVEHYWDL 134
RWD smart00591
domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily ...
 2-68 1.42e-09

domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain;


Pssm-ID: 214735  Cd Length: 107  Bit Score: 53.90  E-value: 1.42e-09
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1016470456    2 VKIDLQVTMPHSYPYVALQLFGRSSE-LDRHQQLLLNKGLTSYIGTFDpGELCVCAAIQWLQDNSASY 68
Cdd:smart00591  41 VSLTLQVKLPENYPDEAPPISLLNSEgLSDEQLAELLKKLEEIAEENL-GEVMIFELVEKLQEFLSEF 107
RWD pfam05773
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 2-65 2.00e-09

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


Pssm-ID: 399058  Cd Length: 111  Bit Score: 53.48  E-value: 2.00e-09
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1016470456   2 VKIDLQVTMPHSYPYVALQLFGRSSE-LDRHQQLLLNKGLTSYIGTFdPGELCVCAAIQWLQDNS 65
Cdd:pfam05773  48 PPLVLKFTLPEDYPDEPPKISLSSPWnLSDEQVLSLLEELEELAEEN-LGEVMIFELIEWLQENL 111
 
Name Accession Description Interval E-value
RWDD2_C cd24163
C-terminal domain of RWD domain-containing protein 2; RWDD2A, as well as RWDD2B, are small ...
 89-218 5.38e-47

C-terminal domain of RWD domain-containing protein 2; RWDD2A, as well as RWDD2B, are small proteins that contain an N-terminal RWD domain and a C-terminal domain of unknown function. The C-terminal domain is predicted (by AlphaFold) to adopt a fold similar to the C-terminal domain of Prp3, an essential U4/U6 di-snRNP-associated protein which plays a role in pre-mRNA splicing. The Prp3 C-terminal domain is a single-stranded RNA-binding domain.


Pssm-ID: 467814  Cd Length: 122  Bit Score: 151.26  E-value: 5.38e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1016470456  89 FLRMWIYSHHIYQQDLRKKILDVGKRLDVTGFCMTGKPGIICVEGFKEHCEEFWHTIRYPNWKHISCKHAESVEtegnge 168
Cdd:cd24163     1 LGRRWIYSHHIYSKKKRKDIVEWAKELGLTGFSKPGKPGVICVEGDEEDVDEYVRRIRRLRWKKIQVRGEEVIE------ 74

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1016470456 169 dlrLFHSFEELLLEAHGDYGLRNDYHMNL-GQFLEFLKKHKSEHVFQILFG 218
Cdd:cd24163    75 ---VPRFFGEEEDEERALPGGFRELHEDLmGELAAFLEEAGLEELFLTLFG 122
RWD_RWDD2 cd23829
RWD domain of RWD domain-containing protein 2A (RWDD2A), 2B (RWDD2B) and related proteins; ...
 1-69 9.31e-28

RWD domain of RWD domain-containing protein 2A (RWDD2A), 2B (RWDD2B) and related proteins; This subfamily includes RWDD2A, previously known as RWD domain-containing 2 (RWDD2) and RWDD2B, previously known in humans as chromosome 21 open reading frame (C21orf6). C21orf6 appears to be involved in monosomy 21 phenotype. The RWD domain may mediate protein-protein interactions.


Pssm-ID: 467663  Cd Length: 129  Bit Score: 102.27  E-value: 9.31e-28
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1016470456   1 MVKIDLQVTMPHSYPYVALQLFGRSSELDRHQQLLLNKGLTSYIGTFDPGELCVCAAIQWLQDNSASYF 69
Cdd:cd23829    61 KVKVELSVTLPHEYPSVPPEIFVRSDSLSRSQQRQLNEDLSEYISSLERGELCILQIVQWLQDNASSYI 129
Prp3_C-like cd24140
C-terminal domain of Prp3 and related proteins; This group contains the C-terminal domains of ...
 89-218 4.52e-19

C-terminal domain of Prp3 and related proteins; This group contains the C-terminal domains of Prp3 and RWD domain-containing proteins 2/3. Predicted structures (by AlphaFold) of the C-terminal domains of RWDD2 and RWDD3 reveal similarity to the C-terminal domain of Prp3, an essential U4/U6 di-snRNP-associated protein which plays a role in pre-mRNA splicing. The Prp3 C-terminal domain is a single-stranded RNA-binding domain.


Pssm-ID: 467812  Cd Length: 117  Bit Score: 79.23  E-value: 4.52e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1016470456  89 FLRMWIYSHHIYQQDLRKKILDVGKRLDVTGFCM-TGKPGIICVEGFKEHCEEFWHTIRYPNWKHISCKHAESVETEGNG 167
Cdd:cd24140     1 YHCKVFHFKHLQNKKKRFKIKENSKELSLKGLCMrIRDPGIIIVVGNEKSCKEYENLVKKRKWNEDFELHTNTGDIKEKC 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1016470456 168 EDLRLFHSFEellleahgdyglrnDYHMNLGQFLEFLKKHKSEHVFQILFG 218
Cdd:cd24140    81 HNNSISKTWE--------------GYLQDCKFKGWFLKVCNDQYSLLRTLG 117
DUF1115 pfam06544
Protein of unknown function (DUF1115); This family represents the C-terminus of hypothetical ...
 93-215 1.12e-13

Protein of unknown function (DUF1115); This family represents the C-terminus of hypothetical eukaryotic proteins of unknown function.


Pssm-ID: 461943  Cd Length: 135  Bit Score: 65.39  E-value: 1.12e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1016470456  93 WIYSHHIYQQDLRKKILDVGKRLDVTGFCMTGKP-GIICVEGFKEHCEEFWHTI-RYPNWKHISCK----HAESVETEGN 166
Cdd:pfam06544   2 VFRIKSLSNPKHRFKIDKNAKQLGLTGFCILGPPfGLVVVEGGEKSIKKYKKLMlRRIKWTENPHEdfeaMKEITFEWDN 81

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1016470456 167 GEDLRLFHSFEELL----LEAHGDYGLRNDYHMNLGQFLEFLKKHKSEHVFQI 215
Cdd:pfam06544  82 DEDNEGEDNKCELVwegeVKERAFRKWKFKVCETEGEAREFLKKHGVEHYWDL 134
RWD smart00591
domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily ...
 2-68 1.42e-09

domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain;


Pssm-ID: 214735  Cd Length: 107  Bit Score: 53.90  E-value: 1.42e-09
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1016470456    2 VKIDLQVTMPHSYPYVALQLFGRSSE-LDRHQQLLLNKGLTSYIGTFDpGELCVCAAIQWLQDNSASY 68
Cdd:smart00591  41 VSLTLQVKLPENYPDEAPPISLLNSEgLSDEQLAELLKKLEEIAEENL-GEVMIFELVEKLQEFLSEF 107
RWD pfam05773
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 2-65 2.00e-09

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


Pssm-ID: 399058  Cd Length: 111  Bit Score: 53.48  E-value: 2.00e-09
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1016470456   2 VKIDLQVTMPHSYPYVALQLFGRSSE-LDRHQQLLLNKGLTSYIGTFdPGELCVCAAIQWLQDNS 65
Cdd:pfam05773  48 PPLVLKFTLPEDYPDEPPKISLSSPWnLSDEQVLSLLEELEELAEEN-LGEVMIFELIEWLQENL 111
RWD_RWDD3 cd23819
RWD domain of RWD domain-containing protein 3 (RWDD3) and related proteins; RWDD3, also called ...
 3-64 2.45e-03

RWD domain of RWD domain-containing protein 3 (RWDD3) and related proteins; RWDD3, also called RWD domain-containing sumoylation enhancer (RSUME), acts as an enhancer of SUMO conjugation and has no effect on ubiquitination. It increases protein sumoylation (a dynamic ubiquitin-like post translational modification) of several proteins including HIF1alpha and I-kappa-B, through direct interaction with UBC9. Its RWD domain is required for the sumoylation enhancement activity.


Pssm-ID: 467655  Cd Length: 106  Bit Score: 36.53  E-value: 2.45e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1016470456   3 KIDLQVTMPHSYPYVALQLFGRSSELDRHQQLLLNKGLTSYIGTFdPGELCVCAAIQWLQDN 64
Cdd:cd23819    44 VLKLTFHLSPNYPSSLPDISVSSEQLTRAQCNDLQDSLLEYANSL-LGEPMVLELVLWLQEN 104
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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