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Conserved domains on  [gi|1206876216|ref|NP_001339377|]
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DDB1- and CUL4-associated factor 4 isoform 7 [Homo sapiens]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1000017)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209
SCOP:  4002744

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 315-434 1.15e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 56.19  E-value: 1.15e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206876216 315 TGHrqsfgtNSDVLAQQFALMAPLLFNGCRSGEIFAIDLRCGNqgkgWKATRLFHDSAVTSVRILQDEQYLMASDMAGKI 394
Cdd:cd00200   174 TGH------TGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGK----CLGTLRGHENGVNSVAFSPDGYLLASGSEDGTI 243

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1206876216 395 KLWDLRTTKCVRQYEGHVNE-YAylpLHVHEEEGILVAGSW 434
Cdd:cd00200   244 RVWDLRTGECVQTLSGHTNSvTS---LAWSPDGKRLASGSA 281
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 315-434 1.15e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 56.19  E-value: 1.15e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206876216 315 TGHrqsfgtNSDVLAQQFALMAPLLFNGCRSGEIFAIDLRCGNqgkgWKATRLFHDSAVTSVRILQDEQYLMASDMAGKI 394
Cdd:cd00200   174 TGH------TGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGK----CLGTLRGHENGVNSVAFSPDGYLLASGSEDGTI 243

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1206876216 395 KLWDLRTTKCVRQYEGHVNE-YAylpLHVHEEEGILVAGSW 434
Cdd:cd00200   244 RVWDLRTGECVQTLSGHTNSvTS---LAWSPDGKRLASGSA 281
WD40 COG2319
WD40 repeat [General function prediction only];
301-434 2.34e-06

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 49.52  E-value: 2.34e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206876216 301 STGLSRRVLLTNVVTGHRQSF--GTNSDVLAQQFALMAPLLFNGCRSGEIFAIDLRCGNQGKGWKAtrlfHDSAVTSVRI 378
Cdd:COG2319   263 SGSADGTVRLWDLATGELLRTltGHSGGVNSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTG----HTGAVRSVAF 338

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1206876216 379 LQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGH---VNEYAYLPlhvheEEGILVAGSW 434
Cdd:COG2319   339 SPDGKTLASGSDDGTVRLWDLATGELLRTLTGHtgaVTSVAFSP-----DGRTLASGSA 392
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 369-398 3.92e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 34.98  E-value: 3.92e-03
                           10        20        30
                   ....*....|....*....|....*....|
gi 1206876216  369 HDSAVTSVRILQDEQYLMASDMAGKIKLWD 398
Cdd:smart00320  11 HTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
369-398 5.03e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 34.63  E-value: 5.03e-03
                          10        20        30
                  ....*....|....*....|....*....|
gi 1206876216 369 HDSAVTSVRILQDEQYLMASDMAGKIKLWD 398
Cdd:pfam00400  10 HTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 315-434 1.15e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 56.19  E-value: 1.15e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206876216 315 TGHrqsfgtNSDVLAQQFALMAPLLFNGCRSGEIFAIDLRCGNqgkgWKATRLFHDSAVTSVRILQDEQYLMASDMAGKI 394
Cdd:cd00200   174 TGH------TGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGK----CLGTLRGHENGVNSVAFSPDGYLLASGSEDGTI 243

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1206876216 395 KLWDLRTTKCVRQYEGHVNE-YAylpLHVHEEEGILVAGSW 434
Cdd:cd00200   244 RVWDLRTGECVQTLSGHTNSvTS---LAWSPDGKRLASGSA 281
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 363-433 4.90e-07

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 51.18  E-value: 4.90e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1206876216 363 KATRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGHVNE-YAylpLHVHEEEGILVAGS 433
Cdd:cd00200   128 LTTLRGHTDWVNSVAFSPDGTFVASSSQDGTIKLWDLRTGKCVATLTGHTGEvNS---VAFSPDGEKLLSSS 196
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 286-413 1.37e-06

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 50.03  E-value: 1.37e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206876216 286 WSCAWSlniQANNCFSTGLS-RRVLLTNVVTG-HRQSF-GTNSDVLAQQFALMAPLLFNGCRSGEIFAIDLRCGNQgkgw 362
Cdd:cd00200    97 SSVAFS---PDGRILSSSSRdKTIKVWDVETGkCLTTLrGHTDWVNSVAFSPDGTFVASSSQDGTIKLWDLRTGKC---- 169

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1206876216 363 KATRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGHVN 413
Cdd:cd00200   170 VATLTGHTGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGKCLGTLRGHEN 220
WD40 COG2319
WD40 repeat [General function prediction only];
301-434 2.34e-06

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 49.52  E-value: 2.34e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206876216 301 STGLSRRVLLTNVVTGHRQSF--GTNSDVLAQQFALMAPLLFNGCRSGEIFAIDLRCGNQGKGWKAtrlfHDSAVTSVRI 378
Cdd:COG2319   263 SGSADGTVRLWDLATGELLRTltGHSGGVNSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTG----HTGAVRSVAF 338

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1206876216 379 LQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGH---VNEYAYLPlhvheEEGILVAGSW 434
Cdd:COG2319   339 SPDGKTLASGSDDGTVRLWDLATGELLRTLTGHtgaVTSVAFSP-----DGRTLASGSA 392
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 310-434 2.67e-06

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 48.87  E-value: 2.67e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206876216 310 LTNVVTGHRqsfgtnSDVLAQQFALMAPLLFNGCRSGEIFAIDLRCGNQgkgwKATRLFHDSAVTSVRILQDEQYLMASD 389
Cdd:cd00200     1 LRRTLKGHT------GGVTCVAFSPDGKLLATGSGDGTIKVWDLETGEL----LRTLKGHTGPVRDVAASADGTYLASGS 70

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1206876216 390 MAGKIKLWDLRTTKCVRQYEGH---VNEYAYLPLHvheeeGILVAGSW 434
Cdd:cd00200    71 SDKTIRLWDLETGECVRTLTGHtsyVSSVAFSPDG-----RILSSSSR 113
WD40 COG2319
WD40 repeat [General function prediction only];
285-411 2.93e-05

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 46.06  E-value: 2.93e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206876216 285 AWSCAWSLNIQANNCFSTGLSRRVLLTNVVTGHRQSFGTNSDVLAQQFALMAPLLFNGCRSGEIFAIDLrcgnQGKGWKA 364
Cdd:COG2319    39 VASLAASPDGARLAAGAGDLTLLLLDAAAGALLATLLGHTAAVLSVAFSPDGRLLASASADGTVRLWDL----ATGLLLR 114

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1206876216 365 TRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGH 411
Cdd:COG2319   115 TLTGHTGAVRSVAFSPDGKTLASGSADGTVRLWDLATGKLLRTLTGH 161
WD40 COG2319
WD40 repeat [General function prediction only];
369-411 3.09e-05

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 46.06  E-value: 3.09e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 1206876216 369 HDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGH 411
Cdd:COG2319   245 HSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTLTGH 287
WD40 COG2319
WD40 repeat [General function prediction only];
369-411 3.91e-05

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 45.67  E-value: 3.91e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 1206876216 369 HDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGH 411
Cdd:COG2319   161 HSGAVTSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGH 203
WD40 COG2319
WD40 repeat [General function prediction only];
369-411 7.17e-05

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 44.90  E-value: 7.17e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 1206876216 369 HDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGH 411
Cdd:COG2319   203 HTGAVRSVAFSPDGKLLASGSADGTVRLWDLATGKLLRTLTGH 245
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 369-398 3.92e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 34.98  E-value: 3.92e-03
                           10        20        30
                   ....*....|....*....|....*....|
gi 1206876216  369 HDSAVTSVRILQDEQYLMASDMAGKIKLWD 398
Cdd:smart00320  11 HTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
369-398 5.03e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 34.63  E-value: 5.03e-03
                          10        20        30
                  ....*....|....*....|....*....|
gi 1206876216 369 HDSAVTSVRILQDEQYLMASDMAGKIKLWD 398
Cdd:pfam00400  10 HTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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