BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily C members KCNC1 and KCNC3; KCNC1 (also called NGK2, voltage-gated potassium channel subunit Kv3.1, or voltage-gated potassium channel subunit Kv4) and KCNC3 (also called KSHIIID or voltage-gated potassium channel subunit Kv3.3) play important roles in the rapid repolarization of fast-firing brain neurons. Assuming opened or closed conformations in response to the voltage difference across the membrane, the proteins form tetrameric potassium-selective channels through which potassium ions may pass in accordance with their electrochemical gradient. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNC1 and KCNC3 are alpha subunit that form functional homo- or hetero-tetrameric channels (with other alpha subunits) through their BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARFDYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFE 170
Cdd:cd18414     4 RIVINVGGTRHETYRSTLRTLPGTRLAWLAEPDAHSNFDYDPRADEFFFDRHPGVFAHILNYYRTGKLHCPADVCGPLYE 83

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1877821666 171 EELGFWGIDETDVEACCWMTYRQHRDAEEALDSF 204
Cdd:cd18414    84 EELAFWGIDETDVEPCCWMTYRQHRDAEEALDSF 117

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily C members KCNC1 and KCNC3; KCNC1 (also called NGK2, voltage-gated potassium channel subunit Kv3.1, or voltage-gated potassium channel subunit Kv4) and KCNC3 (also called KSHIIID or voltage-gated potassium channel subunit Kv3.3) play important roles in the rapid repolarization of fast-firing brain neurons. Assuming opened or closed conformations in response to the voltage difference across the membrane, the proteins form tetrameric potassium-selective channels through which potassium ions may pass in accordance with their electrochemical gradient. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNC1 and KCNC3 are alpha subunit that form functional homo- or hetero-tetrameric channels (with other alpha subunits) through their BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARFDYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFE 170
Cdd:cd18414     4 RIVINVGGTRHETYRSTLRTLPGTRLAWLAEPDAHSNFDYDPRADEFFFDRHPGVFAHILNYYRTGKLHCPADVCGPLYE 83

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1877821666 171 EELGFWGIDETDVEACCWMTYRQHRDAEEALDSF 204
Cdd:cd18414    84 EELAFWGIDETDVEPCCWMTYRQHRDAEEALDSF 117

BTB/POZ domain; In voltage-gated K+ channels this domain is responsible for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. In KCTD1 this domain functions as a transcriptional repressor. It also mediates homomultimerization of KCTD1 and interaction of KCTD1 with the transcription factor AP-2-alpha.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAaarFDYDPGTDEFFFDRHPGVFAYVLNYYRT-GKLHCPADVCGPLFE 170
Cdd:pfam02214   1 VKLNVGGTRFETLKSTLTRFPDTRLGRLLELEC---DDYDDDTNEYFFDRSPKHFETILNFYRTgGKLHRPEEVCLDEFL 77

                          90
                  ....*....|....*.
gi 1877821666 171 EELGFWGIDETDVEAC 186
Cdd:pfam02214  78 EEAEFYGLDELALELC 93

voltage-gated potassium channel protein;

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1877821666 490 NIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPV-----PVIVNNF 543
Cdd:PRK10537  168 SLSTAFYFSIVTMSTVGYGDIVPVSESARLFTISVIILGITVFATSIsaifgPVIRGNL 226

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily C members KCNC1 and KCNC3; KCNC1 (also called NGK2, voltage-gated potassium channel subunit Kv3.1, or voltage-gated potassium channel subunit Kv4) and KCNC3 (also called KSHIIID or voltage-gated potassium channel subunit Kv3.3) play important roles in the rapid repolarization of fast-firing brain neurons. Assuming opened or closed conformations in response to the voltage difference across the membrane, the proteins form tetrameric potassium-selective channels through which potassium ions may pass in accordance with their electrochemical gradient. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNC1 and KCNC3 are alpha subunit that form functional homo- or hetero-tetrameric channels (with other alpha subunits) through their BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARFDYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFE 170
Cdd:cd18414     4 RIVINVGGTRHETYRSTLRTLPGTRLAWLAEPDAHSNFDYDPRADEFFFDRHPGVFAHILNYYRTGKLHCPADVCGPLYE 83

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1877821666 171 EELGFWGIDETDVEACCWMTYRQHRDAEEALDSF 204
Cdd:cd18414    84 EELAFWGIDETDVEPCCWMTYRQHRDAEEALDSF 117

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily C members KCNC2 and KCNC4; KCNC2, also called Shaw-like potassium channel or voltage-gated potassium channel Kv3.2, is a delayed rectifier voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain. It contributes to the regulation of the fast action potential repolarization and in sustained high-frequency firing in neurons of the central nervous system. KCNC4, also called KSHIIIC or voltage-gated potassium channel subunit Kv3.4, is a novel high-voltage-activating, tetraethylammonium (TEA)-sensitive, type-A potassium channel that mediates the voltage-dependent potassium ion permeability of excitable membranes. It plays a pivotal role in oxidative stress-related neural cell damage as an oxidation-sensitive channel. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNC2 and KCNC4 are alpha subunit that form functional homo- or hetero-tetrameric channels (with other alpha subunits) through their BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAGLTEPE-----------AAARFDYDPGTDEFFFDRHPGVFAYVLNYYRTGKLH 159
Cdd:cd18415     3 RIILNVGGTRHETYRSTLKTLPGTRLALLASSDpqgdcltqcsqVGGSDGSPGGGNEFFFDRHPGVFAYVLNYYRTGKLH 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1877821666 160 CPADVCGPLFEEELGFWGIDETDVEACCWMTYRQHRDAEEAL 201
Cdd:cd18415    83 CPADVCGPLFEEELAFWGIDETDVEPCCWMTYRQHRDAEEAL 124

Ion transport protein; This family contains sodium, potassium and calcium ion channels. This family is 6 transmembrane helices in which the last two helices flank a loop which determines ion selectivity. In some sub-families (e.g. Na channels) the domain is repeated four times, whereas in others (e.g. K channels) the protein forms as a tetramer in the membrane.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666 290 ARYVAFASLFFILISITTFCLETHEGFIHISNKtvtqaspipgappenitnvevetepFLTYVEGVCVVWFTFEFLMRVT 369
Cdd:pfam00520   1 SRYFELFILLLILLNTIFLALETYFQPEEPLTT-------------------------VLEILDYVFTGIFTLEMLLKII 55

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666 370 FCPDKVEFLKSSLNIIDCVAILPFYLEVGLSGLSSkaakdvLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEF 449
Cdd:pfam00520  56 AAGFKKRYFRSPWNILDFVVVLPSLISLVLSSVGS------LSGLRVLRLLRLLRLLRLIRRLEGLRTLVNSLIRSLKSL 129

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666 450 LLLIIFLALGVLIFATMIY----YAERIGADPDDilgsNHTYFKNIPIGFWWAVVTMTTLGYGDMYPKTWSGM------- 518
Cdd:pfam00520 130 GNLLLLLLLFLFIFAIIGYqlfgGKLKTWENPDN----GRTNFDNFPNAFLWLFQTMTTEGWGDIMYDTIDGKgefwayi 205

                         250       260       270
                  ....*....|....*....|....*....|...
gi 1877821666 519 LVGALCALAGVLTIAMPVPVIVNNFGMYYSLAM 551
Cdd:pfam00520 206 YFVSFIILGGFLLLNLFIAVIIDNFQELTERTE 238

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in voltage-gated potassium (Kv) channels; Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. This family includes several groups of alpha subunits such as KCNA/Kv1 family of Shaker-type Kv channels, KCNB/Kv2 family of Shab-type Kv channels, KCNC/Kv3 family of Shaw-type Kv channels, KCND/Kv4 family of Shal-type Kv channels, KCNF/Kv5 subfamily of Kv channels, KCNG/Kv6 subfamily of Kv channels, KCNV/Kv8 subfamily of Kv channels, and KCNS/Kv9 subfamily of Kv channels. Kv alpha subunits form functional homo- or hetero-tetrameric channels (typically with other alpha subunits from the same subfamily) through their BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif. KCNQ/Kv7 channels are not included in this family, since they do not contain a BTB/POZ domain.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAGLTEpeaaARFDYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFEE 171
Cdd:cd18317     1 VVLNVGGTRFELSRSTLLRFPDTRLGKLAK----ESHAYDESTNEYFFDRNPEVFEAILDYYRTGELHLPSNVCPASFKE 76

                  ....*.
gi 1877821666 172 ELGFWG 177
Cdd:cd18317    77 ELEFWG 82

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in KCND/Kv4 subfamily of Shal-type voltage-dependent potassium channels; Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. The potassium voltage-gated channel subfamily Kv4, also known as subfamily D, contains three alpha subunit members, Kv4.1 (KCND1), Kv4.2 (KCND2), and Kv4.3 (KCND3), which are orthologs of the Shal gene in Drosophila. They are A-type potassium channels that mediate the native, fast inactivating (A-type) K+ current (IA) described both in the nervous system (A currents) and the heart (transient outward current). Kv4/KCND subfamily alpha subunits form functional homo- or hetero-tetrameric channels through their BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif. They are modulated by cytoplasmic KChIPs/KCNIPs (Kv-channel interacting proteins), which are small calcium binding proteins with EF-hand-like domains.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTrLAGLTEPEaaarFDYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFE 170
Cdd:cd18380     1 RIVINVSGRRFETWKNTLEKYPDT-LLGSTEKE----FFYDDDTKEYFFDRDPEIFRHILNFYRTGKLHYPRHECISAYD 75

                          90       100
                  ....*....|....*....|....*
gi 1877821666 171 EELGFWGIDETDVEACCWMTYRQHR 195
Cdd:cd18380    76 DELAFFGILPEIIGDCCYEDYRDRK 100

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily B member 2 (KCNB2); KCNB2, also called voltage-gated potassium channel subunit Kv2.2, mediates transmembrane potassium transport in excitable membranes, primarily in the brain and smooth muscle cells. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a tetrameric potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. KCNB2 contributes to the delayed-rectifier voltage-gated potassium current in cortical pyramidal neurons and smooth muscle cells. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNB2 is an alpha subunit that forms functional homo- or hetero-tetrameric channels (with other alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARF-----DYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVC 165
Cdd:cd18412    18 RVKINVGGLNHEVLWRTLDRLPRTRLGKLRDCNTHESLlevcdDYNLNENEYFFDRHPGAFTSILNFYRTGKLHMMEEMC 97

                          90       100       110
                  ....*....|....*....|....*....|
gi 1877821666 166 GPLFEEELGFWGIDETDVEACCWMTYRQHR 195
Cdd:cd18412    98 ALSFGQELDYWGIDEIYLESCCQARYHQKK 127

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily S member 1 (KCNS1); KCNS1, also called delayed-rectifier K(+) channel alpha subunit 1 or voltage-gated potassium channel subunit Kv9.1, is a modulatory alpha subunit of voltage-gated potassium channel that mediates neuropathic pain following nerve injury. It can form functional heterotetrameric channels with KCNB1 (also known as Kv2.1) and KCNB2 (also known as Kv2.2), and further modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1 and KCNB2. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNS1 is a regulatory alpha subunit that cannot form a functional homo-tetrameric channel. It forms hetero-tetrameric channels (with other functional alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARF-----DYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCG 166
Cdd:cd18426     1 ININVGGLKRRLSSSTLSKFPDTRLGRLLSCDSEESIlqicdDYDVSAKEFYFDRNPGLFPYVLHFYQTGKLHIMEELCV 80

                          90       100
                  ....*....|....*....|....*...
gi 1877821666 167 PLFEEELGFWGIDETDVEACCwmTYRQH 194
Cdd:cd18426    81 FSFSQEIEYWGINEFFLDSCC--SYRYH 106

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily D member 1 (KCND1); KCND1, also called voltage-gated potassium channel subunit Kv4.1, is a pore-forming subunit of voltage-gated rapidly inactivating A-type potassium channels. It may contribute to I (To) current in heart and I (Sa) current in neurons. Its properties are modulated by interactions with other alpha subunits and with regulatory subunits. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCND1 is an alpha subunit that forms functional homo- or hetero-tetrameric channels (with other Kv4/KCND alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif. It is modulated by cytoplasmic KChIPs/KCNIPs (Kv-channel interacting proteins), which are small calcium binding proteins with EF-hand-like domains.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTrLAGLTEPEaaarFDYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFEE 171
Cdd:cd18417    37 LVVNVSGRRFQTWKNTLDRYPDT-LLGSSEKE----FFYNEDTQEYFFDRDPEMFRHILNFYRTGRLHYPRHECIQAFDE 111

                          90       100
                  ....*....|....*....|....
gi 1877821666 172 ELGFWGIDETDVEACCWMTYRQHR 195
Cdd:cd18417   112 ELSFYGIIPEIIGDCCLEEYRDRK 135

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in KCNS/Kv9 subfamily of potassium voltage-gated channels; Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. The potassium voltage-gated channel subfamily Kv9, also known as subfamily S, includes KCNS1 (Kv9.1), KCNS2 (Kv9.2) and KCNS3 (Kv9.3). They are regulatory alpha subunits that cannot form functional homo-tetrameric channels. Both KCNS1 and KCNS2 are delayed-rectifier K(+) channel alpha subunits that can form functional heterotetrameric channels with KCNB1 (also known as Kv2.1) and KCNB2 (also known as Kv2.2), and further modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1 and KCNB2. KCNS3 is a delayed-rectifier K(+) channel alpha subunit linked to tissue oxygenation responses. It can form functional heterotetrameric channels with KCNB1, and further modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAGL---TEPEAAARF--DYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCG 166
Cdd:cd18384     1 IRINVGGFRRRLSASALLRFPGTRLGRLlacHSEEAILELcdDYDVAAREFYFDRNPGFFLYVLHFYRTGKLHVMDELCV 80

                          90       100
                  ....*....|....*....|....*...
gi 1877821666 167 PLFEEELGFWGIDETDVEACCwmTYRQH 194
Cdd:cd18384    81 FSFSQEIEYWGINEFFLDSCC--SYRYH 106

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily S member 2 (KCNS2); KCNS2, also called delayed-rectifier K(+) channel alpha subunit 2 or voltage-gated potassium channel subunit Kv9.2, is a modulatory alpha subunit of voltage-gated potassium channel that can form functional heterotetrameric channels with KCNB1 (also known as Kv2.1) and KCNB2 (also known as Kv2.2), and further modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1 and KCNB2. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNS2 is a regulatory alpha subunit that cannot form a functional homo-tetrameric channel. It forms hetero-tetrameric channels (with other functional alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARF-----DYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVC 165
Cdd:cd18427     1 EISINVGGFKKRLRSHTLLRFPETRLGRLLSCRSKESIlelcdDYDDTKNEFYFDRNPELFPYVLHFYNTGKLHVMGELC 80

                          90       100
                  ....*....|....*....|....*..
gi 1877821666 166 GPLFEEELGFWGIDETDVEACCWMTYR 192
Cdd:cd18427    81 VFSFSQEIEYWGINEFFIDSCCSYSYH 107

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily V member 1 (KCNV1); KCNV1, also called neuronal potassium channel alpha subunit HNKA or voltage-gated potassium channel subunit Kv8.1, is a new neuronal voltage-gated potassium channel alpha subunit with specific inhibitory properties towards Shab and Shaw channels. It modulates KCNB1 (also known as Kv2.1) and KCNB2 (also known as Kv2.2) channel activity by shifting the threshold for inactivation to more negative values and by slowing the rate of inactivation. It can also down-regulate the channel activity of KCNB1, KCNB2, KCNC4 (also known as Kv3.4) and KCND1 (also known as Kv4.1), possibly by trapping them in intracellular membranes. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNV1 is a regulatory alpha subunit that cannot form a functional homo-tetrameric channel. It forms hetero-tetrameric channels (with other functional alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARF----DYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGP 167
Cdd:cd18424     1 FTINVGGSRFVLSQQVLSSHPETRLGKLAVSVRDSVLelcdDANFVDNEYFFDRSSQAFKYIMNYYKTGHLHVMEELCAI 80

                          90       100
                  ....*....|....*....|....
gi 1877821666 168 LFEEELGFWGIDETDVEACCWMTY 191
Cdd:cd18424    81 SFLQEIEYWGIDELSIDSCCRERY 104

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily G members, KCNG1 and KCNG2; KCNG1, also called voltage-gated potassium channel subunit Kv6.1 or kH2, functions as a regulatory alpha-subunit of voltage-gated potassium channel that can form functional heterotetrameric channels with KCNB1 (also known as Kv2.1), and further modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1. KCNG2, also called cardiac potassium channel subunit or voltage-gated potassium channel subunit Kv6.2, is a new gamma-subunit of voltage-gated potassium channels that can form functional heterodimeric channels with KCNB1, and further modulates channel activity by shifting the threshold and the half-maximal activation to more negative values. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNG1 and KCNG2 are regulatory alpha subunits and do not form homomultimers. They form heteromultimers (with other alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARF-----DYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCG 166
Cdd:cd18421     6 VIINVGGIKYRIPWTTLEEFPLTRLGQLKSCNNFDEImdicdDYDVTCNEFFFDRNPCAFRTILTFLRAGKLRLLREMCA 85

                          90       100
                  ....*....|....*....|....*..
gi 1877821666 167 PLFEEELGFWGIDETDVEACCWMTYRQ 193
Cdd:cd18421    86 LSFQEELLYWGIEEENLEWCCRRRLLQ 112

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily G member 4 (KCNG4); KCNG4, also called voltage-gated potassium channel subunit Kv6.4, is a silent voltage-gated potassium (KvS) channel subunit that can form functional heterotetrameric channels with KCNB1 (also known as Kv2.1), and further modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNG4 is a regulatory alpha subunit that cannot form a functional homo-tetrameric channel. It forms hetero-tetrameric channels (with other functional alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARF-----DYDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVC 165
Cdd:cd18423     3 EIIINVGGIKYLMPWSTLDEFPLTRLGKLKFCSSYEEIvqicdDYDEDTNEFFFDRNPCAFRMIVSFLAAGKLRLLREMC 82

                          90       100
                  ....*....|....*....|..
gi 1877821666 166 GPLFEEELGFWGIDETDVEACC 187
Cdd:cd18423    83 ALSFQEELTYWGIEEANLERCC 104

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium channel tetramerization domain-containing proteins; The potassium channel tetramerization domain (KCTD) family proteins contain the BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif that facilitates homodimerization or heterodimerization. KCTD proteins play crucial roles in a variety of fundamental biological processes, such as protein ubiquitination and degradation, suppression of proliferation or transcription, cytoskeleton regulation, tetramerization and gating of ion channels and others. Some KCTD proteins are involved in protein ubiquitination as part of the CRL (Cullin RING Ligase) E3 ligases. Some others show Cullin-independent functions including binding and regulation of GABA (gamma-aminobutyric acid) receptors (KCTD8, KCTD12 and KCTD16) and inhibition of AP-2 function (KCTD15). KCTD family BTB domains can adopt a wide range of oligomerization geometries, including homodimerization, tetramerization, and pentamerization.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARFDYDpgtDEFFFDRHPGVFAYVLNYYRTGKLHCPAD 163
Cdd:cd18316     1 VKLNVGGTLFTTSRSTLLKDPDSLLAALFSGRWPLPRDED---GSIFIDRDPELFRHILNFLRTGKLPLPSD 69

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily G member 3 (KCNG3); KCNG3, also called voltage-gated potassium channel subunit Kv6.3 or voltage-gated potassium channel subunit Kv10.1, is an electrically silent modulatory subunit that can form functional heterotetrameric channels with KCNB1 (also known as Kv2.1), and further promotes a reduction in the rate of activation and inactivation of the delayed rectifier voltage-gated potassium channel KCNB1. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNG3 is a regulatory alpha subunit that cannot form a functional homo-tetrameric channel. It forms hetero-tetrameric channels (with other functional alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAGL----TEPEAAARFD-YDPGTDEFFFDRHPGVFAYVLNYYRTGKLHCPADVCG 166
Cdd:cd18422     1 VTLNVGGTRYSFSREVIKDFPLRRVSRLhgclSEQDVLEVCDdYDRERNEYFFDRHSEAFGFIMLYVKYGKLRFVPQMCE 80

                          90       100
                  ....*....|....*....|.
gi 1877821666 167 PLFEEELGFWGIDETDVEACC 187
Cdd:cd18422    81 LSFYNEMIYWGLERSHLEYCC 101

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in KCNA/Kv1 subfamily of Shaker-type voltage-dependent potassium channels; Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. The potassium voltage-gated channel subfamily Kv1, also known as subfamily A, contains eight alpha subunit members, Kv1.1 (KCNA1), Kv1.2 (KCNA2), Kv1.3 (KCNA3), Kv1.4 (KCNA4), Kv1.5 (KCNA5), Kv1.6 (KCNA6), Kv1.7 (KCNA7), and Kv1.8 (KCNA10), which are orthologs of the Shaker gene in Drosophila. They are delayed rectifiers except for Kv1.4 (KCNA4), which is an A-type potassium channel. Delayed rectifiers are slow opening and closing voltage-gated potassium channels. Because of their delayed activation kinetics, they play an important role in controlling action potential duration. A-type channels are fast/rapidly inactivating potassium channels. Kv1/KCNA subfamily alpha subunits form functional homo- or hetero-tetrameric channels (with other Kv1/KCNA alpha subunits) through their BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAgltEPEAAARFdYDPGTDEFFFDRHPGVFAYVLNYYRT-GKLHCPADVCGPLF 169
Cdd:cd18377     2 RVVINVSGLRFETQLKTLAQFPNTLLG---DPNKRMRF-FDPLRNEYFFDRNRPSFDAILYYYQSgGRLRRPVNVPLDIF 77

                  ....*..
gi 1877821666 170 EEELGFW 176
Cdd:cd18377    78 LEEIRFY 84

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Drosophila melanogaster potassium voltage-gated channel protein Shaker and similar proteins; Shaker, also termed protein minisleep, represents a family of putative potassium channel proteins in the nervous system of Drosophila. It is a voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a tetrameric potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Shaker plays a role in the regulation of sleep need or efficiency. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. Shaker is an alpha subunit that forms functional homo- or hetero-tetrameric channels (with other alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAgltEPEAAARFdYDPGTDEFFFDRHPGVFAYVLNYYRT-GKLHCPADVCGPLF 169
Cdd:cd18410     2 RVVINVSGLRFETQLKTLNQFPDTLLG---DPQRRNRY-YDPLRNEYFFDRNRPSFDAILYYYQSgGRLRRPVNVPLDVF 77

                          90
                  ....*....|....*
gi 1877821666 170 EEELGFWGIDETDVE 184
Cdd:cd18410    78 SEEIKFYELGENAFE 92

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily A member 4 (KCNA4); KCNA4 is also called HPCN2, or voltage-gated K(+) channel HuKII, voltage-gated potassium channel HBK4, voltage-gated potassium channel HK1, or voltage-gated potassium channel subunit Kv1.4. It mediates transmembrane potassium transport in excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a tetrameric potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNA4 is an alpha subunit that forms functional homo- or hetero-tetrameric channels (with other Kv1/KCNA alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAgltEPEAAARFdYDPGTDEFFFDRHPGVFAYVLNYYRT-GKLHCPADVCGPLF 169
Cdd:cd18405     2 RVVINVSGLRFETQLKTLAQFPETLLG---DPEKRMRY-FDPLRNEYFFDRNRPSFDAILYYYQSgGRLKRPVNVPFDIF 77

                          90
                  ....*....|.
gi 1877821666 170 EEELGFWGIDE 180
Cdd:cd18405    78 SEEVKFYELGE 88

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily A member 10 (KCNA10); KCNA10, also called voltage-gated potassium channel subunit Kv1.8, is a cyclic nucleotide-gated, voltage-activated potassium channel that mediates transmembrane potassium transport in excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a tetrameric potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. KCNA10 is expressed in proximal tubular cells, glomerular and vascular endothelial cells, as well as in vascular smooth muscle cells. It may facilitate proximal tubular sodium absorption by stabilizing cell membrane voltage. The channel activity is up-regulated by cAMP. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNA10 is an alpha subunit that forms functional homotetrameric channels through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAgltEPEAAARFdYDPGTDEFFFDRHPGVFAYVLNYYRT-GKLHCPADVCGPLF 169
Cdd:cd18409     2 KVIINIAGLRFETQLKTLDQFPDTLLG---DPEKRMKY-FDSMRNEYFFDRNRPSFDGILYYYQSgGKIRRPANVPLDVF 77

                  ....*..
gi 1877821666 170 EEELGFW 176
Cdd:cd18409    78 ADEIIFY 84

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium voltage-gated channel subfamily A member 6 (KCNA6); KCNA6, also called voltage-gated potassium channel HBK2 or voltage-gated potassium channel subunit Kv1.6, mediates transmembrane potassium transport in excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a tetrameric potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. KCNA6 is distributed primarily in neurons of central and peripheral nervous systems. Voltage-gated potassium (Kv) channels are composed of alpha subunits, which form the actual conductance pore, and cytoplasmic beta subunits, which are auxiliary proteins that associate with alpha subunits to modulate the activity of the Kv channel. KCNA6 is an alpha subunit that forms functional homo- or hetero-tetrameric channels (with other Kv1/KCNA alpha subunits) through its BTB/POZ domain, also known as tetramerization (T1) domain, which is a versatile protein-protein interaction motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  91 KIVINVGGVRHETYRSTLRTLPGTRLAgltEPEAAARFdYDPGTDEFFFDRHPGVFAYVLNYYRT-GKLHCPADVCGPLF 169
Cdd:cd18407     2 RLVINISGLRFETQLRTLSIFPDTLLG---DPSRRVRY-FDPLRNEYFFDRNRPSFDAILYYYQSgGRLRRPVHVPLDIF 77

                          90
                  ....*....|....*.
gi 1877821666 170 EEELGFWGIDETDVEA 185
Cdd:cd18407    78 LEEIRFYQLGQEAIET 93

Ion channel; This family includes the two membrane helix type ion channels found in bacteria.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666 460 VLIFATMIYYAErigadpddilgsnHTYFKNIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVI 539
Cdd:pfam07885   6 VLIFGTVYYLLE-------------EGWEWSFLDALYFSFVTLTTVGYGDIVPLTDAGRLFTIFYILIGIPLFAIFLAVL 72

                  ..
gi 1877821666 540 VN 541
Cdd:pfam07885  73 GR 74

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in SH3KBP1-binding protein 1 (SHKBP1); SHKBP1, also called SETA-binding protein 1, interacts with cathepsin B and participates in tumor necrosis factor (TNF)-induced apoptosis in ovarian cancer cells. It can promote epidermal growth factor receptor (EGFR) signaling by interrupting c-Cbl-CIN85 complex and inhibiting EGFR degradation. It contains a BTB/POZ domain, also known as tetramerization (T1) domain, a versatile protein-protein interaction motif that facilitates homodimerization or heterodimerization. KCTD family BTB domains can adopt a wide range of oligomerization geometries, including homodimerization, tetramerization, and pentamerization.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARFDYdpgTDEFFFDRHPGVFAYVLNYYRTGKLHcPADVCGPLFEE 171
Cdd:cd18393     4 IHLNVGGKRFSTSRQTLTWIPDSFFSSLLSGRISTLKDE---TGAIFIDRDPTVFAPILNFLRTKELD-PRGIHVSLLLH 79

                  ....*..
gi 1877821666 172 ELGFWGI 178
Cdd:cd18393    80 EAQFYGI 86

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium channel tetramerization domain-containing proteins, KCTD8, KCTD12, KCTD16 and similar proteins; This subfamily of KCTD proteins includes KCTD8, KCTD12 (also called predominantly fetal expressed T1 domain/Pfetin), and KCTD16. They act as auxiliary subunits of GABAB receptors associated with mood disorders. KCTD8 interacts as a tetramer with GABRB1 and GABRB2. KCTD12 regulates agonist potency and kinetics of GABAB receptor signaling. It promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition. KCTD16 interacts with amyloid beta precursor protein (APP), a type I transmembrane protein involved in a variety of cellular processes such as cell adhesion, and axon guidance. The BTB/POZ domain, also known as tetramerization (T1) domain, is a versatile protein-protein interaction motif that facilitates homodimerization or heterodimerization. KCTD family BTB domains can adopt a wide range of oligomerization geometries, including homodimerization, tetramerization, and pentamerization.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1877821666  94 INVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARFDYDpGTDEFFFDRHPGVFAYVLNYYRTGKLHCP 161
Cdd:cd18367     7 LNVGGQVYTTSLSTLIKDPDSLLGRMFSGKNRQELARD-SKGRYFLDRDGVLFRYILDYLRNQKLVLP 73

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium channel tetramerization domain-containing proteins, KCTD6, KCTD21 and similar proteins; KCTD6, also called KCASH3 (KCTD containing, Cullin3 adaptor, suppressor of Hedgehog 3), is a substrate-specific adaptor of cullin-3, effectively regulating protein levels of the muscle small ankyrin-1 isoform 5 (sAnk1.5). KCTD21, also called KCASH2, functions as a substrate-specific adaptor of cullin-3, promoting the ubiquitination and degradation of histone deacetylase HDAC1, thereby inhibiting the deacetylation-mediated transcriptional activation of the Hedgehog effectors Gli1 and Gli2. The BTB/POZ domain, also known as tetramerization (T1) domain, is a versatile protein-protein interaction motif that facilitates homodimerization or heterodimerization. KCTD family BTB domains can adopt a wide range of oligomerization geometries, including homodimerization, tetramerization, and pentamerization.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAgltepeaaARF--DYDPGTDE---FFFDRHPGVFAYVLNYYRTGKLHCPAD 163
Cdd:cd18365     3 VNLNVGGVHYTTTLATLTRFPDSMLG--------AMFsgSMPTTRDDqgnYFIDRDGTLFRYILNFLRSSQLSLPED 71

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in potassium channel tetramerization domain-containing protein 4 (KCTD4); KCTD4 is a BTB/POZ domain-containing protein with an unknown biological function. KCTD proteins play crucial roles in a variety of fundamental biological processes, such as protein ubiquitination and degradation, suppression of proliferation or transcription, cytoskeleton regulation, tetramerization and gating of ion channels and others. Some KCTD proteins are involved in protein ubiquitination as part of the CRL (Cullin RING Ligase) E3 ligases. The BTB/POZ domain, also known as tetramerization (T1) domain, is a versatile protein-protein interaction motif that facilitates homodimerization or heterodimerization. KCTD family BTB domains can adopt a wide range of oligomerization geometries, including homodimerization, tetramerization, and pentamerization.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1877821666  92 IVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARFDYDpgtDEFFFDRHPGVFAYVLNYYRTGKLHCPAD 163
Cdd:cd18364     2 VTLNVGGYLYITQRQTLTKYPDSFLEGMVNGKIQCTVDAD---GNYFIDRDGLLFRHVLNFLRNGELLLPEG 70