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Conserved domains on  [gi|194248077|ref|NP_004226|]
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Krueppel-like factor 4 isoform 2 [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 7-396 8.11e-116

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


:

Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 343.99  E-value: 8.11e-116
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077   7 ESDMAVSDALLPSFSTFASGPAGREKTLRQaGAPNNRWREELSHMKRLPPVLPGRPYDlaaatvatdlesggagaacggS 86
Cdd:cd21582    1 EFDMALSGTLLPSISTFASGPAVKEKALGQ-GSANNRWREELSHLKRPCPPLGGRCYD---------------------Q 58

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077  87 NLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSspsssgpasapsTCSFTYPIRAGNDPGVapgg 166
Cdd:cd21582   59 DPPLGMRRKEEEFNDLLDYDFILSNSLLQQQQQQQQAASTSSSTSSSS------------SSPYSYQLPSPQGTAT---- 122

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 167 tgggllygreSAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQpqppgggLMGKFVLKASLSAPGSEYGsps 246
Cdd:cd21582  123 ----------SFLYPIPRANLADINDVSPSGGFVAELMRPDLDPAYLQPTS-------LHGKFVVKTTMDMGDYSQS--- 182

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 247 vISVSKGSPdgsHPVVVAPYNGGPPRTCPKIKQEAVSSCTHlgAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEV-- 324
Cdd:cd21582  183 -INVSKSAP---MTKSSVAPSSSLPFMCPRIKQENPSTCTI--SRPMDGHLGGNSQHGFSQRAPLPSRTTPSGGPGGGns 256

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 325 -----LSSRDCHP------ALPLPPGFHPHPGPNYPSFLPDQMQPQvpplHYQELMPPGSCMPEEPKPKRGRRSWPRKRT 393
Cdd:cd21582  257 staesLMSRDHHPssqvlsHPPLPLPQGYHPSPGYPPFPPPPSQPQ----QYQELMSPGSCLPEEPKPKRGRRSWPRKRT 332


                 ...
gi 194248077 394 ATH 396
Cdd:cd21582  333 ATH 335
zf-H2C2_2 pfam13465
Zinc-finger double domain;
442-467 1.08e-05

Zinc-finger double domain;


:

Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 41.97  E-value: 1.08e-05
                          10        20
                  ....*....|....*....|....*.
gi 194248077  442 ELTRHYRKHTGHRPFQCQKCDRAFSR 467
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
391-474 1.17e-04

FOG: Zn-finger [General function prediction only];


:

Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 44.30  E-value: 1.17e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 391 KRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCdRAFSRSDH 470
Cdd:COG5048  382 KNDKKSETLSNSCIRNFKRDSNLSLHIITHLSFRPYNCKNPPCSKSFNRHYNLIPHKKIHTNHAPLLCSIL-KSFRRDLD 460


                 ....
gi 194248077 471 LALH 474
Cdd:COG5048  461 LSNH 464
 
Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 7-396 8.11e-116

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 343.99  E-value: 8.11e-116
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077   7 ESDMAVSDALLPSFSTFASGPAGREKTLRQaGAPNNRWREELSHMKRLPPVLPGRPYDlaaatvatdlesggagaacggS 86
Cdd:cd21582    1 EFDMALSGTLLPSISTFASGPAVKEKALGQ-GSANNRWREELSHLKRPCPPLGGRCYD---------------------Q 58

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077  87 NLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSspsssgpasapsTCSFTYPIRAGNDPGVapgg 166
Cdd:cd21582   59 DPPLGMRRKEEEFNDLLDYDFILSNSLLQQQQQQQQAASTSSSTSSSS------------SSPYSYQLPSPQGTAT---- 122

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 167 tgggllygreSAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQpqppgggLMGKFVLKASLSAPGSEYGsps 246
Cdd:cd21582  123 ----------SFLYPIPRANLADINDVSPSGGFVAELMRPDLDPAYLQPTS-------LHGKFVVKTTMDMGDYSQS--- 182

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 247 vISVSKGSPdgsHPVVVAPYNGGPPRTCPKIKQEAVSSCTHlgAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEV-- 324
Cdd:cd21582  183 -INVSKSAP---MTKSSVAPSSSLPFMCPRIKQENPSTCTI--SRPMDGHLGGNSQHGFSQRAPLPSRTTPSGGPGGGns 256

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 325 -----LSSRDCHP------ALPLPPGFHPHPGPNYPSFLPDQMQPQvpplHYQELMPPGSCMPEEPKPKRGRRSWPRKRT 393
Cdd:cd21582  257 staesLMSRDHHPssqvlsHPPLPLPQGYHPSPGYPPFPPPPSQPQ----QYQELMSPGSCLPEEPKPKRGRRSWPRKRT 332


                 ...
gi 194248077 394 ATH 396
Cdd:cd21582  333 ATH 335
zf-H2C2_2 pfam13465
Zinc-finger double domain;
442-467 1.08e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 41.97  E-value: 1.08e-05
                          10        20
                  ....*....|....*....|....*.
gi 194248077  442 ELTRHYRKHTGHRPFQCQKCDRAFSR 467
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
391-474 1.17e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 44.30  E-value: 1.17e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 391 KRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCdRAFSRSDH 470
Cdd:COG5048  382 KNDKKSETLSNSCIRNFKRDSNLSLHIITHLSFRPYNCKNPPCSKSFNRHYNLIPHKKIHTNHAPLLCSIL-KSFRRDLD 460


                 ....
gi 194248077 471 LALH 474
Cdd:COG5048  461 LSNH 464
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 396-420 4.27e-04

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 37.28  E-value: 4.27e-04
                          10        20
                  ....*....|....*....|....*
gi 194248077  396 HTCDYagCGKTYTKSSHLKAHLRTH 420
Cdd:pfam00096   1 YKCPD--CGKSFSRKSNLKRHLRTH 23
ZnF_C2H2 smart00355
zinc finger;
456-478 4.83e-03

zinc finger;


Pssm-ID: 197676  Cd Length: 23  Bit Score: 34.36  E-value: 4.83e-03
                           10        20
                   ....*....|....*....|...
gi 194248077   456 FQCQKCDRAFSRSDHLALHMKRH 478
Cdd:smart00355   1 YRCPECGKVFKSKSALREHMRTH 23
 
Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 7-396 8.11e-116

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 343.99  E-value: 8.11e-116
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077   7 ESDMAVSDALLPSFSTFASGPAGREKTLRQaGAPNNRWREELSHMKRLPPVLPGRPYDlaaatvatdlesggagaacggS 86
Cdd:cd21582    1 EFDMALSGTLLPSISTFASGPAVKEKALGQ-GSANNRWREELSHLKRPCPPLGGRCYD---------------------Q 58

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077  87 NLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSspsssgpasapsTCSFTYPIRAGNDPGVapgg 166
Cdd:cd21582   59 DPPLGMRRKEEEFNDLLDYDFILSNSLLQQQQQQQQAASTSSSTSSSS------------SSPYSYQLPSPQGTAT---- 122

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 167 tgggllygreSAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQpqppgggLMGKFVLKASLSAPGSEYGsps 246
Cdd:cd21582  123 ----------SFLYPIPRANLADINDVSPSGGFVAELMRPDLDPAYLQPTS-------LHGKFVVKTTMDMGDYSQS--- 182

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 247 vISVSKGSPdgsHPVVVAPYNGGPPRTCPKIKQEAVSSCTHlgAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEV-- 324
Cdd:cd21582  183 -INVSKSAP---MTKSSVAPSSSLPFMCPRIKQENPSTCTI--SRPMDGHLGGNSQHGFSQRAPLPSRTTPSGGPGGGns 256

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 325 -----LSSRDCHP------ALPLPPGFHPHPGPNYPSFLPDQMQPQvpplHYQELMPPGSCMPEEPKPKRGRRSWPRKRT 393
Cdd:cd21582  257 staesLMSRDHHPssqvlsHPPLPLPQGYHPSPGYPPFPPPPSQPQ----QYQELMSPGSCLPEEPKPKRGRRSWPRKRT 332


                 ...
gi 194248077 394 ATH 396
Cdd:cd21582  333 ATH 335
KLF1_2_4_N cd21972
N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins; Kruppel ...
 144-396 5.14e-21

N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins; Kruppel/Krueppel-like transcription factors (KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the related N-terminal domains of KLF1, KLF2, KLF4, and similar proteins.


Pssm-ID: 409230 [Multi-domain]  Cd Length: 194  Bit Score: 90.82  E-value: 5.14e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 144 APSTCSFTYPIRAGndpgvapggtgggllygresappptapfnladindVSPSGGFVAELLRPELDPVYIPPQQpqppgg 223
Cdd:cd21972   13 KPEDDLSKFLDLEF-----------------------------------ILSNTVTSDNDNPPPPDPAYPPPES------ 51

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 224 glmgkfvlKASLSAPGSEYGSPSVISVSKGSPDGSHP--VVVApynGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPA 301
Cdd:cd21972   52 --------PESCSTVYDSDGCHPTPNAYCGPNGPGLPghFLLA---GNSPNLGPKIKTENQEQACMPVAGYSGHYGPREP 120

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 302 AHDFPLgrqlpsrttptlgleevlssrdcHPALPlppgfhphpgpNYPSFLPDQMQPQVPPLHYQ-------------EL 368
Cdd:cd21972  121 QRVPPA-----------------------PPPPQ-----------YAGHFQYHGHFNMFSPPLRAnhpgmstvmltplST 166

                        250       260
                 ....*....|....*....|....*...
gi 194248077 369 MPPGSCMPEEPKPKRGRRSWPRKRTATH 396
Cdd:cd21972  167 PPLGFLSPEEAKPKRGRRSWARKRTATH 194
KLF2_N cd21583
N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as ...
 13-396 7.17e-21

N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as Krueppel-like factor 2 or lung Kruppel-like Factor/LKLF) is a protein that, in humans, is encoded by the KLF2 gene on chromosome 19. It has been implicated in a variety of biochemical processes in the human body, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF2 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF2, which is related to the N-terminal domains of KLF1 and KLF4.


Pssm-ID: 409229 [Multi-domain]  Cd Length: 299  Bit Score: 92.79  E-value: 7.17e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077  13 SDALLPSFSTFASGpagREKtlrqagAPNNRWREELSHMkrlppvlpgrpydlaaatvatdleSGGAGAACGGSNLAPLP 92
Cdd:cd21583    1 SGTILPSISTFANQ---KEK------CWEDRWKGEEDKS------------------------SLSSCLLDLQNGDSCLG 47

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077  93 RRETEEFNDLLDLDFILSNSLTHPPesvaatvsssasassSSSPSSSGPASAPSTCSFTYPiraGNDPGVAPGGTGggll 172
Cdd:cd21583   48 RKDDEDLDNYLDLDFILANTAGSDS---------------AGAAGGGYYGSLPEPAAPYSA---SNPPGAYSVPEQ---- 105

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 173 ygreSAPPPtapfnladindvsPSGGFVAELLRPELDPVYippqqpqPPGGGLMGKFVLKaslSAPGSEYGSPSVISVSk 252
Cdd:cd21583  106 ----GSPPP-------------PYSSSLMAELLRDSDSDY-------GPSNSIQGRFLVS---SAFFPRQDDPDSIKVE- 157

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 253 GSPDGSHPVVvapynGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLgrqLPsrttPTLgleevLSSRDCHP 332
Cdd:cd21583  158 PSMDSYGPVM-----GMVPQSCPKIKQEGNVSCMMSFEQPRLANSPQAAGSMTPP---LS----PDD-----LMASECQQ 220

                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 194248077 333 ALPLPPGFHPHPGPNYPSFLPDQMQPQ-------------VPPLHYQELMPPGSCMPE--EPKPKRGRRSWPRKRTATH 396
Cdd:cd21583  221 QMMCSPSFPQPHHPHYPPPPQPPLQYQsphqfglfedglpLQPSAQRVLLTPPSSPLEllESKPKRGRRSWPRKRTATH 299
zf-H2C2_2 pfam13465
Zinc-finger double domain;
442-467 1.08e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 41.97  E-value: 1.08e-05
                          10        20
                  ....*....|....*....|....*.
gi 194248077  442 ELTRHYRKHTGHRPFQCQKCDRAFSR 467
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
KLF1_2_4_N-like cd22056
N-terminal domain of Kruppel-like factors with similarity to the N-terminal domains of ...
 15-396 1.27e-05

N-terminal domain of Kruppel-like factors with similarity to the N-terminal domains of Kruppel-like factor (KLF)1, KLF2, and KLF4; Kruppel/Krueppel-like transcription factors (KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domains of an unknown subfamily of KLFs, predominantly found in fish, related to the N-terminal domains of KLF1, KLF2, and KLF4.


Pssm-ID: 409231 [Multi-domain]  Cd Length: 339  Bit Score: 47.35  E-value: 1.27e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077  15 ALLPSFSTFASGPAGREKT---LRQAGAPNNRWREELSHMKrlPPVLPGRPYDlaaatvatdlesggagaacggsNLAPL 91
Cdd:cd22056    1 AMLPSISTFASLQPLEEKQpeiLCRWSVDAASSEVEFSIVQ--SPIEVPKDDD----------------------ELGKF 56

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077  92 PRREtEEFNDLLDLDFILSN----------------SLTHPPEsvaatvsssasassssspsssgpasapsTCSFTYpir 155
Cdd:cd22056   57 LDEE-DILSNFLDLEFILSSsngsssgpsqqqqcayPLPESPE----------------------------SCSTGS--- 104

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 156 agnDPgvapggtgggllygrESAPPPTAPFNLADINDVSPSGGFVAELLRPELdpvyippQQPQPPGGGLMGKFVlkasl 235
Cdd:cd22056  105 ---DS---------------DGSDAPQPYHGSAGFTSSSPVHSLMAELLTPEM-------NYPGESSPDSAGKAR----- 154

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 236 saPGSEYGSPSVISVskgspdgSHPVVVAPYNGGPPRtcPKIKQE--------AVSSCTHLGA---GPPLSNGHRPAAHD 304
Cdd:cd22056  155 --DGREYTELRALPT-------APPAHQPATSPPPLG--YKIKTEpveqscmmAAGGGGFMGQqkpKHQMHSVHPQAFTH 223

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 305 FPLGRQLPSRTTPTLGLEEVL---SSRDCHPALPLppgFHPHPGPNYPSFLPDQMQPQVP--------PLHYQELMPPGS 373
Cdd:cd22056  224 HQAAGPGALQGRGGRGGPDCHllhSSHHHHHHHHL---QYQYMNAPYPPHYAHQGAPQFHgqysvfrePMRVHHQGHPGS 300

                        410       420       430
                 ....*....|....*....|....*....|....*....
gi 194248077 374 CM----------------PEEPKPKRGRRSWPRKRTATH 396
Cdd:cd22056  301 MLtppssppllefyaqdePEDIKPKRGRRSWARKRTATH 339
KLF1_N cd21581
N-terminal domain of Kruppel-like Factor 1; Kruppel-like Factor 1 (KLF1, also known as ...
 349-396 2.39e-05

N-terminal domain of Kruppel-like Factor 1; Kruppel-like Factor 1 (KLF1, also known as Krueppel-like factor 1 or Erythroid Kruppel-like Factor/EKLF) was the first Kruppel-like factor discovered. It was found to be vitally important for embryonic erythropoiesis in promoting the switch from fetal hemoglobin (Hemoglobin F) to adult hemoglobin (Hemoglobin A) gene expression by binding to highly conserved CACCC domains. EKLF ablation in mouse embryos produces a lethal anemic phenotype, causing death by embryonic day 14, and natural mutations lead to beta+ thalassemia in humans. However, expression of embryonic hemoglobin and fetal hemoglobin genes is normal in EKLF-deficient mice, suggesting other factors may be involved. KLF1 functions as a transcriptional activator. It belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF1, which is related to the N-terminal domains of KLF2 and KLF4.


Pssm-ID: 409227 [Multi-domain]  Cd Length: 278  Bit Score: 45.81  E-value: 2.39e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 194248077 349 PSFLPDQMQPQVP---PLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATH 396
Cdd:cd21581  228 AALFPDYAHSPGPghlPLGQQPLLPDPPLPPGGAEGKRGRRSWAKKRPAVH 278
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 456-478 3.65e-05

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 40.36  E-value: 3.65e-05
                          10        20
                  ....*....|....*....|...
gi 194248077  456 FQCQKCDRAFSRSDHLALHMKRH 478
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
391-474 1.17e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 44.30  E-value: 1.17e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194248077 391 KRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCdRAFSRSDH 470
Cdd:COG5048  382 KNDKKSETLSNSCIRNFKRDSNLSLHIITHLSFRPYNCKNPPCSKSFNRHYNLIPHKKIHTNHAPLLCSIL-KSFRRDLD 460


                 ....
gi 194248077 471 LALH 474
Cdd:COG5048  461 LSNH 464
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 396-420 4.27e-04

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 37.28  E-value: 4.27e-04
                          10        20
                  ....*....|....*....|....*
gi 194248077  396 HTCDYagCGKTYTKSSHLKAHLRTH 420
Cdd:pfam00096   1 YKCPD--CGKSFSRKSNLKRHLRTH 23
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
403-461 1.41e-03

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 41.22  E-value: 1.41e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 194248077 403 CGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKC 461
Cdd:COG5048   39 CTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHLRTHHNNPSDLNSKS 97
zf-H2C2_2 pfam13465
Zinc-finger double domain;
412-439 2.29e-03

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 35.42  E-value: 2.29e-03
                          10        20
                  ....*....|....*....|....*...
gi 194248077  412 HLKAHLRTHTGEKPYHCdwDGCGWKFAR 439
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKC--PECGKSFKS 26
ZnF_C2H2 smart00355
zinc finger;
456-478 4.83e-03

zinc finger;


Pssm-ID: 197676  Cd Length: 23  Bit Score: 34.36  E-value: 4.83e-03
                           10        20
                   ....*....|....*....|...
gi 194248077   456 FQCQKCDRAFSRSDHLALHMKRH 478
Cdd:smart00355   1 YRCPECGKVFKSKSALREHMRTH 23
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 426-450 6.85e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.20  E-value: 6.85e-03
                          10        20
                  ....*....|....*....|....*
gi 194248077  426 YHCDwdGCGWKFARSDELTRHYRKH 450
Cdd:pfam00096   1 YKCP--DCGKSFSRKSNLKRHLRTH 23
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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