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Conserved domains on  [gi|4758148|ref|NP_004392|]
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DNA fragmentation factor subunit alpha isoform 1 [Homo sapiens]

Protein Classification

CIDE_N and DFF-C domain-containing protein( domain architecture ID 10245149)

CIDE_N and DFF-C domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DFF-C pfam09033
DNA Fragmentation factor 45kDa, C terminal domain; The C terminal domain of DNA Fragmentation ...
 100-264 8.04e-84

DNA Fragmentation factor 45kDa, C terminal domain; The C terminal domain of DNA Fragmentation factor 45kDa (DFF-C) consists of four alpha-helices, which are folded in a helix-packing arrangement, with alpha-2 and alpha-3 packing against a long C-terminal helix (alpha-4). The main function of this domain is the inhibition of DFF40 by binding to its C-terminal catalytic domain through ionic interactions, thereby inhibiting the fragmentation of DNA in the apoptotic process. In addition to blocking the DNase activity of DFF40, the C-terminal region of DFF45 is also important for the DFF40-specific folding chaperone activity, as demonstrated by the ability of DFF45 to refold DFF40.


:

Pssm-ID: 462661  Cd Length: 164  Bit Score: 250.36  E-value: 8.04e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758148    100 DGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVL 179
Cdd:pfam09033   1 DGGTAWLSQESVEVDEVDSGAGEKWKNLARQLKQDLSSIILMSEEDLQVLIDVPCSDLAQELGQSEAKVQGLQDTLQQVL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758148    180 DQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGiSRETSSDVALASHILTALREKQAPELSLSSQD 259
Cdd:pfam09033  81 DRREEERQSKQLLQLYLKALEKEGSILSKQSESKAAPGEEVDVVDTG-SMEVDSASGFSSRTLMVLKEKTSPELRLSSQD 159


                  ....*
gi 4758148    260 LELVT 264
Cdd:pfam09033 160 LELVV 164
CIDE_N super family cl02541
CIDE_N domain, found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) ...
 18-96 5.77e-42

CIDE_N domain, found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) proteins, as well as CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of ICAD/DFF45, and the CAD/DFF40 and CIDE nucleases during apoptosis. The CIDE-N domain is also found in the FSP27/CIDE-C protein.


The actual alignment was detected with superfamily member cd06536:

Pssm-ID: 470607  Cd Length: 80  Bit Score: 140.32  E-value: 5.77e-42
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 4758148   18 LKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAY 96
Cdd:cd06536   2 LKPCVVCNVSRQKQHGVAASSLEELRIKACESLGFDSSSAPITLVLAEDGTIVEDEDYFLCLPPNTKFVLLAENEKWAP 80
 
Name Accession Description Interval E-value
DFF-C pfam09033
DNA Fragmentation factor 45kDa, C terminal domain; The C terminal domain of DNA Fragmentation ...
 100-264 8.04e-84

DNA Fragmentation factor 45kDa, C terminal domain; The C terminal domain of DNA Fragmentation factor 45kDa (DFF-C) consists of four alpha-helices, which are folded in a helix-packing arrangement, with alpha-2 and alpha-3 packing against a long C-terminal helix (alpha-4). The main function of this domain is the inhibition of DFF40 by binding to its C-terminal catalytic domain through ionic interactions, thereby inhibiting the fragmentation of DNA in the apoptotic process. In addition to blocking the DNase activity of DFF40, the C-terminal region of DFF45 is also important for the DFF40-specific folding chaperone activity, as demonstrated by the ability of DFF45 to refold DFF40.


Pssm-ID: 462661  Cd Length: 164  Bit Score: 250.36  E-value: 8.04e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758148    100 DGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVL 179
Cdd:pfam09033   1 DGGTAWLSQESVEVDEVDSGAGEKWKNLARQLKQDLSSIILMSEEDLQVLIDVPCSDLAQELGQSEAKVQGLQDTLQQVL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758148    180 DQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGiSRETSSDVALASHILTALREKQAPELSLSSQD 259
Cdd:pfam09033  81 DRREEERQSKQLLQLYLKALEKEGSILSKQSESKAAPGEEVDVVDTG-SMEVDSASGFSSRTLMVLKEKTSPELRLSSQD 159


                  ....*
gi 4758148    260 LELVT 264
Cdd:pfam09033 160 LELVV 164
CIDE_N_ICAD cd06536
CIDE_N domain of ICAD. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) ...
 18-96 5.77e-42

CIDE_N domain of ICAD. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of the CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD (DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of the CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and release of active DFF40/CAD nuclease.


Pssm-ID: 119369  Cd Length: 80  Bit Score: 140.32  E-value: 5.77e-42
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 4758148   18 LKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAY 96
Cdd:cd06536   2 LKPCVVCNVSRQKQHGVAASSLEELRIKACESLGFDSSSAPITLVLAEDGTIVEDEDYFLCLPPNTKFVLLAENEKWAP 80
CAD smart00266
Domains present in proteins implicated in post-mortem DNA fragmentation;
19-94 8.97e-33

Domains present in proteins implicated in post-mortem DNA fragmentation;


Pssm-ID: 128562  Cd Length: 74  Bit Score: 116.27  E-value: 8.97e-33
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 4758148      19 KPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSltPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKW 94
Cdd:smart00266   1 RPFKVRDHDRNVRKGVAASSLEELLSKVCDKLALPDS--PVTLVLEEDGTIVDDEEYFQTLPDNTELMALEKGEKW 74
CIDE-N pfam02017
CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. ...
 19-95 1.43e-29

CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. The two proteins interact through this domain.


Pssm-ID: 460413  Cd Length: 75  Bit Score: 107.97  E-value: 1.43e-29
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 4758148     19 KPCLLRRNYSREQHGVAASCLEDLRSKACDILAIdkSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWA 95
Cdd:pfam02017   1 KPFKVCNVDRSRKKGVAASSLEELLQKGCEKLLL--PSEPVTLVLEEDGTEVDDEEYFQTLPDNTELMLLEKGEKWT 75
 
Name Accession Description Interval E-value
DFF-C pfam09033
DNA Fragmentation factor 45kDa, C terminal domain; The C terminal domain of DNA Fragmentation ...
 100-264 8.04e-84

DNA Fragmentation factor 45kDa, C terminal domain; The C terminal domain of DNA Fragmentation factor 45kDa (DFF-C) consists of four alpha-helices, which are folded in a helix-packing arrangement, with alpha-2 and alpha-3 packing against a long C-terminal helix (alpha-4). The main function of this domain is the inhibition of DFF40 by binding to its C-terminal catalytic domain through ionic interactions, thereby inhibiting the fragmentation of DNA in the apoptotic process. In addition to blocking the DNase activity of DFF40, the C-terminal region of DFF45 is also important for the DFF40-specific folding chaperone activity, as demonstrated by the ability of DFF45 to refold DFF40.


Pssm-ID: 462661  Cd Length: 164  Bit Score: 250.36  E-value: 8.04e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758148    100 DGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVL 179
Cdd:pfam09033   1 DGGTAWLSQESVEVDEVDSGAGEKWKNLARQLKQDLSSIILMSEEDLQVLIDVPCSDLAQELGQSEAKVQGLQDTLQQVL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758148    180 DQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGiSRETSSDVALASHILTALREKQAPELSLSSQD 259
Cdd:pfam09033  81 DRREEERQSKQLLQLYLKALEKEGSILSKQSESKAAPGEEVDVVDTG-SMEVDSASGFSSRTLMVLKEKTSPELRLSSQD 159


                  ....*
gi 4758148    260 LELVT 264
Cdd:pfam09033 160 LELVV 164
CIDE_N_ICAD cd06536
CIDE_N domain of ICAD. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) ...
 18-96 5.77e-42

CIDE_N domain of ICAD. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of the CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD (DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of the CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and release of active DFF40/CAD nuclease.


Pssm-ID: 119369  Cd Length: 80  Bit Score: 140.32  E-value: 5.77e-42
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 4758148   18 LKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAY 96
Cdd:cd06536   2 LKPCVVCNVSRQKQHGVAASSLEELRIKACESLGFDSSSAPITLVLAEDGTIVEDEDYFLCLPPNTKFVLLAENEKWAP 80
CAD smart00266
Domains present in proteins implicated in post-mortem DNA fragmentation;
19-94 8.97e-33

Domains present in proteins implicated in post-mortem DNA fragmentation;


Pssm-ID: 128562  Cd Length: 74  Bit Score: 116.27  E-value: 8.97e-33
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 4758148      19 KPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSltPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKW 94
Cdd:smart00266   1 RPFKVRDHDRNVRKGVAASSLEELLSKVCDKLALPDS--PVTLVLEEDGTIVDDEEYFQTLPDNTELMALEKGEKW 74
CIDE-N pfam02017
CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. ...
 19-95 1.43e-29

CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. The two proteins interact through this domain.


Pssm-ID: 460413  Cd Length: 75  Bit Score: 107.97  E-value: 1.43e-29
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 4758148     19 KPCLLRRNYSREQHGVAASCLEDLRSKACDILAIdkSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWA 95
Cdd:pfam02017   1 KPFKVCNVDRSRKKGVAASSLEELLQKGCEKLLL--PSEPVTLVLEEDGTEVDDEEYFQTLPDNTELMLLEKGEKWT 75
CIDE_N cd01615
CIDE_N domain, found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) ...
 18-95 2.84e-27

CIDE_N domain, found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) proteins, as well as CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of ICAD/DFF45, and the CAD/DFF40 and CIDE nucleases during apoptosis. The CIDE-N domain is also found in the FSP27/CIDE-C protein.


Pssm-ID: 119367  Cd Length: 78  Bit Score: 101.85  E-value: 2.84e-27
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 4758148   18 LKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDksLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWA 95
Cdd:cd01615   2 LRPFKVCDSDRSRKKGVAASSLEELLSKACEKLKLP--SAPVTLVLEEDGTEVDDEEYFQTLPDNTVLMLLEPGQKWT 77
CIDE_N_FSP27 cd06538
CIDE_N domain of FSP27 proteins. The CIDE-N (cell death-inducing DFF45-like effector, ...
 18-95 4.87e-13

CIDE_N domain of FSP27 proteins. The CIDE-N (cell death-inducing DFF45-like effector, N-terminal) domain is found in the FSP27/CIDE-C protein, which has been identified as a n adipocyte lipid droplet protein that negatively regulates lipolysis and promotes triglyceride accumulation. The CIDE protein family includes 3 members: CIDE-A, CIDE-B, and FSP27(CIDE-C). Based on sequence similarity with DFF40 and DFF45, CIDE proteins were initially characterized as mitochondrial activators of apoptosis. The CIDE-N domain of FSP27 is sufficient to increase apoptosis in vitro when overexpressed.


Pssm-ID: 119371  Cd Length: 79  Bit Score: 63.68  E-value: 4.87e-13
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 4758148   18 LKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKsltPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWA 95
Cdd:cd06538   2 PRPFRVSNADRSLRKGIMADSLEDLLNKVLDALLLDC---ISSLVLDEDGTGVDTEEFFQALADNTVFMVLGKGQKWK 76
CIDE_N_A cd06539
CIDE_N domain of CIDE-A proteins. The CIDE_N (cell death-inducing DFF45-like effector, ...
 26-94 3.51e-12

CIDE_N domain of CIDE-A proteins. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) proteins. These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of the CAD/DFF40, ICAD/DFF45, and CIDE nucleases during apoptosis. The CIDE protein family includes 3 members: CIDE-A, CIDE-B, and FSP27(CIDE-C). Based on sequence similarity with DFF40 and DFF45, the CIDE proteins were initially characterized as mitochondrial activators of apoptosis. However, strong metabolic phenotypes of mice lacking CIDE-A and CIDE-B indicated that this family may play critical roles in energy balance.


Pssm-ID: 119372  Cd Length: 78  Bit Score: 61.11  E-value: 3.51e-12
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758148   26 NYSRE-QHGVAASCLEDLRSKACDILAIDKSLtpVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKW 94
Cdd:cd06539   9 NHDRSsRRGVMASSLQELISKTLDALVITSGL--VTLVLEEDGTVVDTEEFFQTLGDNTHFMVLEKGQKW 76
CIDE_N_CAD cd06535
CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) ...
 18-94 4.01e-07

CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and the release of active DFF40/CAD nuclease.


Pssm-ID: 119368  Cd Length: 77  Bit Score: 47.03  E-value: 4.01e-07
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 4758148   18 LKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDksLTPVTLVLAEDGTIVdDDDYFLCLPSNTKFVALASNEKW 94
Cdd:cd06535   2 PKCVKIRSLNSAQKYGVAAKNLKELLRKGCRLLQLP--CAGSRLCLYEDGTEV-TEEYFPTLPDNTELVLLTPGQSW 75
CIDE_N_B cd06537
CIDE_N domain of CIDE-B proteins. The CIDE_N (cell death-inducing DFF45-like effector, ...
 33-95 3.91e-06

CIDE_N domain of CIDE-B proteins. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) proteins. These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of the CAD/DFF40, ICAD/DFF45 and CIDE nucleases during apoptosis. The CIDE protein family includes 3 members: CIDE-A, CIDE-B, and FSP27(CIDE-C). Based on sequence similarity with DFF40 and DFF45, CIDE proteins were initially characterized as mitochondrial activators of apoptosis. However, strong metabolic phenotypes of mice lacking CIDE-A and CIDE-B indicated that this family may play critical roles in energy balance.


Pssm-ID: 119370  Cd Length: 81  Bit Score: 44.14  E-value: 3.91e-06
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 4758148   33 GVAASCLEDLRSKACDILAIDKSLTpvtLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWA 95
Cdd:cd06537  17 GLTAASLQELLAKALETLLLSGVLT---LVLEEDGTAVDSEDFFELLEDDTCLMVLEQGQSWS 76
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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