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Conserved domains on  [gi|6323242|ref|NP_013314|]
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putative glycosylase [Saccharomyces cerevisiae S288C]

Protein Classification

ChtBD1_GH16 and GH16_fungal_CRH1_transglycosylase domain-containing protein( domain architecture ID 10161223)

ChtBD1_GH16 and GH16_fungal_CRH1_transglycosylase domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GH16_fungal_CRH1_transglycosylase cd02183
glycosylphosphatidylinositol-glucanosyltransferase; Group of fungal GH16 members related to ...
 143-354 2.40e-103

glycosylphosphatidylinositol-glucanosyltransferase; Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.


:

Pssm-ID: 185692 [Multi-domain]  Cd Length: 203  Bit Score: 304.86  E-value: 2.40e-103
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  143 EAEKMLEDFDFT-HSGYTSIeaSSGNIVLAMPKKTTGSLITSTRSFLYGKASVRMKTARSRGVVTAFDLTSAIGDEIDFE 221
Cdd:cd02183   2 DFTGNASSYDWTvTSGTVDY--DDDGASLTIPKRGDGPTISSTFYIFYGKVEVTMKAAPGQGIVSSFVLQSDDLDEIDWE 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  222 WLGGDLMTAQSNYYSQGHL-DYTRMQRFPVGADTWATYHTYEIDWDPDRIIWYVDGKIARTVLKKDTwdpiSKEYRYPQT 300
Cdd:cd02183  80 WVGGDLTQVQTNYFGKGNTtTYDRGGYHPVPNPQTEEFHTYTIDWTKDRITWYIDGKVVRTLTKADT----TGGYGYPQT 155

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....
gi 6323242  301 PMRLEIAVWPGGSETNGPGTINWAGGLIDWenspdiiEKGQFTAHVEQITVTPY 354
Cdd:cd02183 156 PMRLQIGIWAGGDPSNAPGTIEWAGGETDY-------DKGPFTMYVKSVTVTDY 202
ChtBD1_GH16 cd06923
Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 16 glycosyl ...
 29-75 4.08e-28

Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 16 glycosyl hydrolases; This subfamily includes Saccharomyces cerevisiae Utr2p, also known as Crh2p, which participates in the cross-linking of chitin to beta(1-3)- and beta(1-6) glucan in the cell wall, and S. cerevisiae Crr1p, a putative transglycosidase which is needed for proper spore wall assembly. ChtBD1 is a lectin domain found in proteins from plants and fungi that bind N-acetylglucosamine, plant endochitinases, wound-induced proteins, and the alpha subunit of Kluyveromyces lactis killer toxin. This domain is involved in the recognition and/or binding of chitin subunits; it typically occurs N-terminal to glycosyl hydrolase domains in chitinases, together with other carbohydrate-binding domains, or by itself in tandem-repeat arrangements.


:

Pssm-ID: 211314  Cd Length: 47  Bit Score: 104.76  E-value: 4.08e-28
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 6323242   29 ISCSPNNPCPAEWPCCSPYNECGAGPICVGGCNVRSSFDEESCAPIP 75
Cdd:cd06923   1 ITCNETSQCPEEWPCCSQYGECGTGPYCLGGCNPRYSFSLDSCMPMP 47
 
Name Accession Description Interval E-value
GH16_fungal_CRH1_transglycosylase cd02183
glycosylphosphatidylinositol-glucanosyltransferase; Group of fungal GH16 members related to ...
 143-354 2.40e-103

glycosylphosphatidylinositol-glucanosyltransferase; Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.


Pssm-ID: 185692 [Multi-domain]  Cd Length: 203  Bit Score: 304.86  E-value: 2.40e-103
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  143 EAEKMLEDFDFT-HSGYTSIeaSSGNIVLAMPKKTTGSLITSTRSFLYGKASVRMKTARSRGVVTAFDLTSAIGDEIDFE 221
Cdd:cd02183   2 DFTGNASSYDWTvTSGTVDY--DDDGASLTIPKRGDGPTISSTFYIFYGKVEVTMKAAPGQGIVSSFVLQSDDLDEIDWE 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  222 WLGGDLMTAQSNYYSQGHL-DYTRMQRFPVGADTWATYHTYEIDWDPDRIIWYVDGKIARTVLKKDTwdpiSKEYRYPQT 300
Cdd:cd02183  80 WVGGDLTQVQTNYFGKGNTtTYDRGGYHPVPNPQTEEFHTYTIDWTKDRITWYIDGKVVRTLTKADT----TGGYGYPQT 155

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....
gi 6323242  301 PMRLEIAVWPGGSETNGPGTINWAGGLIDWenspdiiEKGQFTAHVEQITVTPY 354
Cdd:cd02183 156 PMRLQIGIWAGGDPSNAPGTIEWAGGETDY-------DKGPFTMYVKSVTVTDY 202
Glyco_hydro_16 pfam00722
Glycosyl hydrolases family 16;
164-325 8.67e-66

Glycosyl hydrolases family 16;


Pssm-ID: 395585 [Multi-domain]  Cd Length: 168  Bit Score: 207.45  E-value: 8.67e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242    164 SSGNIVLAMPKKTtGSLITSTRSFLYGKASVRMKTARSRGVVTAFDLTSAI---GDEIDFEWLGGDLMTAQSNYYSQGHL 240
Cdd:pfam00722   9 SNGGLTLTLDKYT-GSGFQSKFYYLYGKVEARIKAARGAGVVTAFYLSSEDwddHDEIDFEFLGNDTGQVQTNVYGNGKG 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242    241 DYTRmQRFPVGADTWATYHTYEIDWDPDRIIWYVDGKIARTVLKKDTWdpiskEYRYPQTPMRLEIAVWPGGSETNGPG- 319
Cdd:pfam00722  88 NRGE-QRFSLWFDPTADFHTYSILWNPDKITWYVDGVPVRTLKNNDAG-----GVPYPQTPMRLYVSLWPGGDWATPGGg 161


                  ....*..
gi 6323242    320 -TINWAG 325
Cdd:pfam00722 162 vKIDWAG 168
ChtBD1_GH16 cd06923
Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 16 glycosyl ...
 29-75 4.08e-28

Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 16 glycosyl hydrolases; This subfamily includes Saccharomyces cerevisiae Utr2p, also known as Crh2p, which participates in the cross-linking of chitin to beta(1-3)- and beta(1-6) glucan in the cell wall, and S. cerevisiae Crr1p, a putative transglycosidase which is needed for proper spore wall assembly. ChtBD1 is a lectin domain found in proteins from plants and fungi that bind N-acetylglucosamine, plant endochitinases, wound-induced proteins, and the alpha subunit of Kluyveromyces lactis killer toxin. This domain is involved in the recognition and/or binding of chitin subunits; it typically occurs N-terminal to glycosyl hydrolase domains in chitinases, together with other carbohydrate-binding domains, or by itself in tandem-repeat arrangements.


Pssm-ID: 211314  Cd Length: 47  Bit Score: 104.76  E-value: 4.08e-28
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 6323242   29 ISCSPNNPCPAEWPCCSPYNECGAGPICVGGCNVRSSFDEESCAPIP 75
Cdd:cd06923   1 ITCNETSQCPEEWPCCSQYGECGTGPYCLGGCNPRYSFSLDSCMPMP 47
BglS COG2273
Beta-glucanase, GH16 family [Carbohydrate transport and metabolism];
176-330 3.26e-24

Beta-glucanase, GH16 family [Carbohydrate transport and metabolism];


Pssm-ID: 441874 [Multi-domain]  Cd Length: 259  Bit Score: 100.83  E-value: 3.26e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  176 TTGSLITSTR-SFLYGKASVRMKTARSRGVVTAF-------DLTSAIGDEIDF-EWLGGDLMTAQSNYY---SQGHLDYT 243
Cdd:COG2273  93 TSGRITTKGKfSFTYGRFEARAKLPKGQGLWPAFwmlggdiDGGWPASGEIDImEFVGKDPNKVHGNVHyggYNGGEGIG 172

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  244 rmQRFPVGADTWATYHTYEIDWDPDRIIWYVDGKIARTVLKKDTWDPiskeyrYP-QTPMRLEIAVWPGGsetNGPGTIN 322
Cdd:COG2273 173 --ASYDLPFDASDDFHTYAVEWTPDSIRWYVDGVLVHTVTPADVGGP------WPfDQPFYLILNLAVGG---NWPGAPD 241

                       170
                ....*....|..
gi 6323242  323 WAGG----LIDW 330
Cdd:COG2273 242 TTGFpatmEVDY 253
PLN03161 PLN03161
Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional
 158-344 1.11e-16

Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional


Pssm-ID: 178706 [Multi-domain]  Cd Length: 291  Bit Score: 79.94  E-value: 1.11e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242   158 YTSIEASSGNIVLAMpKKTTGSLITSTRSFLYGKASVRMK--TARSRGVVTAFDLTSA--IGDEIDFEWLG---GDLMTA 230
Cdd:PLN03161  39 HSSMLGNGDNLQLVL-DQSSGSGIKSKRAFLFGSIEMLIKlvPGNSAGTVTAYYLSSTgsRHDEIDFEFLGnvsGQPYTI 117

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242   231 QSNYYSQGhlDYTRMQRFPVGADTWATYHTYEIDWDPDRIIWYVDGKIARtVLKKDTWDPISkeyrYP-QTPMRLEIAVW 309
Cdd:PLN03161 118 HTNIYTQG--NGSREQQFRPWFDPTADFHNYTIHWNPSEVVWYVDGTPIR-VFRNYENEGIA----YPnKQGMRVYSSLW 190

                        170       180       190
                 ....*....|....*....|....*....|....*....
gi 6323242   310 PGGsetngpgtiNWA--GGL--IDWENSPDIIEKGQFTA 344
Cdd:PLN03161 191 NAD---------NWAtqGGRvkIDWTLAPFVARGRRFRA 220
 
Name Accession Description Interval E-value
GH16_fungal_CRH1_transglycosylase cd02183
glycosylphosphatidylinositol-glucanosyltransferase; Group of fungal GH16 members related to ...
 143-354 2.40e-103

glycosylphosphatidylinositol-glucanosyltransferase; Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.


Pssm-ID: 185692 [Multi-domain]  Cd Length: 203  Bit Score: 304.86  E-value: 2.40e-103
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  143 EAEKMLEDFDFT-HSGYTSIeaSSGNIVLAMPKKTTGSLITSTRSFLYGKASVRMKTARSRGVVTAFDLTSAIGDEIDFE 221
Cdd:cd02183   2 DFTGNASSYDWTvTSGTVDY--DDDGASLTIPKRGDGPTISSTFYIFYGKVEVTMKAAPGQGIVSSFVLQSDDLDEIDWE 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  222 WLGGDLMTAQSNYYSQGHL-DYTRMQRFPVGADTWATYHTYEIDWDPDRIIWYVDGKIARTVLKKDTwdpiSKEYRYPQT 300
Cdd:cd02183  80 WVGGDLTQVQTNYFGKGNTtTYDRGGYHPVPNPQTEEFHTYTIDWTKDRITWYIDGKVVRTLTKADT----TGGYGYPQT 155

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....
gi 6323242  301 PMRLEIAVWPGGSETNGPGTINWAGGLIDWenspdiiEKGQFTAHVEQITVTPY 354
Cdd:cd02183 156 PMRLQIGIWAGGDPSNAPGTIEWAGGETDY-------DKGPFTMYVKSVTVTDY 202
Glyco_hydro_16 pfam00722
Glycosyl hydrolases family 16;
164-325 8.67e-66

Glycosyl hydrolases family 16;


Pssm-ID: 395585 [Multi-domain]  Cd Length: 168  Bit Score: 207.45  E-value: 8.67e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242    164 SSGNIVLAMPKKTtGSLITSTRSFLYGKASVRMKTARSRGVVTAFDLTSAI---GDEIDFEWLGGDLMTAQSNYYSQGHL 240
Cdd:pfam00722   9 SNGGLTLTLDKYT-GSGFQSKFYYLYGKVEARIKAARGAGVVTAFYLSSEDwddHDEIDFEFLGNDTGQVQTNVYGNGKG 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242    241 DYTRmQRFPVGADTWATYHTYEIDWDPDRIIWYVDGKIARTVLKKDTWdpiskEYRYPQTPMRLEIAVWPGGSETNGPG- 319
Cdd:pfam00722  88 NRGE-QRFSLWFDPTADFHTYSILWNPDKITWYVDGVPVRTLKNNDAG-----GVPYPQTPMRLYVSLWPGGDWATPGGg 161


                  ....*..
gi 6323242    320 -TINWAG 325
Cdd:pfam00722 162 vKIDWAG 168
ChtBD1_GH16 cd06923
Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 16 glycosyl ...
 29-75 4.08e-28

Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 16 glycosyl hydrolases; This subfamily includes Saccharomyces cerevisiae Utr2p, also known as Crh2p, which participates in the cross-linking of chitin to beta(1-3)- and beta(1-6) glucan in the cell wall, and S. cerevisiae Crr1p, a putative transglycosidase which is needed for proper spore wall assembly. ChtBD1 is a lectin domain found in proteins from plants and fungi that bind N-acetylglucosamine, plant endochitinases, wound-induced proteins, and the alpha subunit of Kluyveromyces lactis killer toxin. This domain is involved in the recognition and/or binding of chitin subunits; it typically occurs N-terminal to glycosyl hydrolase domains in chitinases, together with other carbohydrate-binding domains, or by itself in tandem-repeat arrangements.


Pssm-ID: 211314  Cd Length: 47  Bit Score: 104.76  E-value: 4.08e-28
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 6323242   29 ISCSPNNPCPAEWPCCSPYNECGAGPICVGGCNVRSSFDEESCAPIP 75
Cdd:cd06923   1 ITCNETSQCPEEWPCCSQYGECGTGPYCLGGCNPRYSFSLDSCMPMP 47
Glyco_hydrolase_16 cd00413
glycosyl hydrolase family 16; The O-Glycosyl hydrolases are a widespread group of enzymes that ...
 176-330 6.35e-27

glycosyl hydrolase family 16; The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.


Pssm-ID: 185683 [Multi-domain]  Cd Length: 210  Bit Score: 106.75  E-value: 6.35e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  176 TTGSLITSTRSFLYGKASVRMKTARSRGVVTAF-----DLTSAIGDEIDFEWLGGDLMTAQSNYYSQGHLDYTRMQRF-- 248
Cdd:cd00413  54 SSAEIDSQKNNYTYGYYEARAKLAGGPGAVSAFwtysdDDDPPDGGEIDIEFLGRDPTTVQTNVHWPGYGAGATTGEEks 133

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  249 -PVGADTWATYHTYEIDWDPDRIIWYVDGKIARTVlkkdtwdpiskEYRYPQTPMRLEIAVW-PGGSETNGPGTINWAGG 326
Cdd:cd00413 134 vHLPFDPADDFHTYRVDWTPGEITFYVDGVLVATI-----------TNQVPDDPMNIILNLWsDGGWWWGGPPPGAPAYM 202


                ....
gi 6323242  327 LIDW 330
Cdd:cd00413 203 EIDW 206
BglS COG2273
Beta-glucanase, GH16 family [Carbohydrate transport and metabolism];
176-330 3.26e-24

Beta-glucanase, GH16 family [Carbohydrate transport and metabolism];


Pssm-ID: 441874 [Multi-domain]  Cd Length: 259  Bit Score: 100.83  E-value: 3.26e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  176 TTGSLITSTR-SFLYGKASVRMKTARSRGVVTAF-------DLTSAIGDEIDF-EWLGGDLMTAQSNYY---SQGHLDYT 243
Cdd:COG2273  93 TSGRITTKGKfSFTYGRFEARAKLPKGQGLWPAFwmlggdiDGGWPASGEIDImEFVGKDPNKVHGNVHyggYNGGEGIG 172

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  244 rmQRFPVGADTWATYHTYEIDWDPDRIIWYVDGKIARTVLKKDTWDPiskeyrYP-QTPMRLEIAVWPGGsetNGPGTIN 322
Cdd:COG2273 173 --ASYDLPFDASDDFHTYAVEWTPDSIRWYVDGVLVHTVTPADVGGP------WPfDQPFYLILNLAVGG---NWPGAPD 241

                       170
                ....*....|..
gi 6323242  323 WAGG----LIDW 330
Cdd:COG2273 242 TTGFpatmEVDY 253
GH16_lichenase cd02175
lichenase, member of glycosyl hydrolase family 16; Lichenase, also known as 1,3-1, ...
 174-353 4.03e-24

lichenase, member of glycosyl hydrolase family 16; Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.


Pssm-ID: 185684 [Multi-domain]  Cd Length: 212  Bit Score: 99.27  E-value: 4.03e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  174 KKTTGSLITSTRSFLYGKASVRMKTARSRGVVTAFDL-TSAIG----DEIDFEWLGGDLMTAQSNYYSQGHLDYTRMqrF 248
Cdd:cd02175  53 KPYACGEYRTRGFYGYGRYEVRMKPAKGSGVVSSFFTyTGPYDgdphDEIDIEFLGKDTTKVQFNYYTNGVGGHEKL--I 130

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  249 PVGADTWATYHTYEIDWDPDRIIWYVDGKIARTVlkKDTWDPIskeyryPQTPMRLEIAVWPGgsetngpgtinwaGGLI 328
Cdd:cd02175 131 DLGFDASEGFHTYAFEWEPDSIRWYVDGELVHEA--TATDPNI------PDTPGKIMMNLWPG-------------DGVD 189

                       170       180
                ....*....|....*....|....*
gi 6323242  329 DWENSPDiiEKGQFTAHVEQITVTP 353
Cdd:cd02175 190 DWLGPFD--GGTPLTAEYDWVSYTP 212
GH16_XET cd02176
Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16; Xyloglucan ...
 175-345 6.01e-23

Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16; Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling.


Pssm-ID: 185685 [Multi-domain]  Cd Length: 263  Bit Score: 97.27  E-value: 6.01e-23
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  175 KTTGSLITSTRSFLYGKASVRMKTAR--SRGVVTAFDLTSAIG---DEIDFEWLG---GDLMTAQSNYYSQGHLDytRMQ 246
Cdd:cd02176  34 QSSGSGFKSKNKYLFGFFSMRIKLPPgdSAGTVTAFYLSSQGPdnhDEIDFEFLGnvtGQPYTLQTNVFANGVGG--REQ 111

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  247 RFPVGADTWATYHTYEIDWDPDRIIWYVDGKIARtVLKKDTwdpiSKEYRYP-QTPMRLEIAVWPGGSetngpgtinWA- 324
Cdd:cd02176 112 RIYLWFDPTADFHTYSILWNPHQIVFYVDDVPIR-VFKNNE----ALGVPYPsSQPMGVYASIWDGSD---------WAt 177

                       170       180
                ....*....|....*....|....
gi 6323242  325 -GGL--IDWENSPdiiekgqFTAH 345
Cdd:cd02176 178 qGGRvkIDWSYAP-------FVAS 194
PLN03161 PLN03161
Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional
 158-344 1.11e-16

Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional


Pssm-ID: 178706 [Multi-domain]  Cd Length: 291  Bit Score: 79.94  E-value: 1.11e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242   158 YTSIEASSGNIVLAMpKKTTGSLITSTRSFLYGKASVRMK--TARSRGVVTAFDLTSA--IGDEIDFEWLG---GDLMTA 230
Cdd:PLN03161  39 HSSMLGNGDNLQLVL-DQSSGSGIKSKRAFLFGSIEMLIKlvPGNSAGTVTAYYLSSTgsRHDEIDFEFLGnvsGQPYTI 117

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242   231 QSNYYSQGhlDYTRMQRFPVGADTWATYHTYEIDWDPDRIIWYVDGKIARtVLKKDTWDPISkeyrYP-QTPMRLEIAVW 309
Cdd:PLN03161 118 HTNIYTQG--NGSREQQFRPWFDPTADFHNYTIHWNPSEVVWYVDGTPIR-VFRNYENEGIA----YPnKQGMRVYSSLW 190

                        170       180       190
                 ....*....|....*....|....*....|....*....
gi 6323242   310 PGGsetngpgtiNWA--GGL--IDWENSPDIIEKGQFTA 344
Cdd:PLN03161 191 NAD---------NWAtqGGRvkIDWTLAPFVARGRRFRA 220
GH16_laminarinase_like cd08023
Laminarinase, member of the glycosyl hydrolase family 16; Laminarinase, also known as glucan ...
 176-330 2.83e-15

Laminarinase, member of the glycosyl hydrolase family 16; Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.


Pssm-ID: 185693 [Multi-domain]  Cd Length: 235  Bit Score: 74.97  E-value: 2.83e-15
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  176 TTGSLITS-TRSFLYGKASVRMKTARSRGVVTAFDLTSAIGD--------EID-FEWLGGDLMTAQSNYYSQGHLDYTR- 244
Cdd:cd08023  65 TSGRITTKgKFSFTYGRVEARAKLPKGQGTWPAFWMLGENIKyvgwpasgEIDiMEYVGNEPNTVYGTLHGGATNDGNNg 144

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  245 -MQRFPVGADTWAT-YHTYEIDWDPDRIIWYVDGKIARTVLKKDTWDPISKEYRYPQTpMRLEIAV---WPGG--SETNG 317
Cdd:cd08023 145 sGGSYTLPTDDLSDdFHTYAVEWTPDKITFYVDGKLYFTYTNPNTDNGGQWPFDQPFY-LILNLAVggnWPGPpdDDTPF 223

                       170
                ....*....|...
gi 6323242  318 PGTInwaggLIDW 330
Cdd:cd08023 224 PATM-----EVDY 231
GH16_beta_agarase cd02178
Beta-agarase, member of glycosyl hydrolase family 16; Beta-agarase is a glycosyl hydrolase ...
 175-306 1.73e-08

Beta-agarase, member of glycosyl hydrolase family 16; Beta-agarase is a glycosyl hydrolase family 16 (GH16) member that hydrolyzes the internal beta-1,4-linkage of agarose, a hydrophilic polysaccharide found in the cell wall of Rhodophyceaea, marine red algae. Agarose is a linear chain of galactose units linked by alternating L-alpha-1,3- and D-beta-1,4-linkages that are additionally modified by a 3,6-anhydro-bridge. Agarose forms thermo-reversible gels that are widely used in the food industry or as a laboratory medium. While beta-agarases are also found in two other families derived from the sequence-based classification of glycosyl hydrolases (GH50, and GH86) the GH16 members are most abundant. This domain adopts a curved beta-sandwich conformation, with a tunnel-shaped active site cavity, referred to as a jellyroll fold.


Pssm-ID: 185687  Cd Length: 258  Bit Score: 55.05  E-value: 1.73e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323242  175 KTTGSLITSTRSFLYGKASVRMKTARSRGVvTAFDLTSAIGD---EID-FEWLGGDLMTAQSN--------YYSQGHLDY 242
Cdd:cd02178  84 KVTTGSITSKEKVKYGYFEARAKASNLPMS-SAFWLLSDTKDsttEIDiLEHYGGDREEWFATrmnsnthvFIRDPEQDY 162

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 6323242  243 TRMQRFP--VGADTWAT-YHTYEIDW-DPDRIIWYVDGKIARTVlkkdtWDPISKEYRYPQTPMRLEI 306
Cdd:cd02178 163 QPKDDGSwyYNPTELADdFHVYGVYWkDPDTIRFYIDGVLVRTV-----ENSEITDGTGFDQPMYIII 225
ChtBD1_GH18_1 cd06922
Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 18 glycosyl ...
 28-61 9.39e-05

Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 18 glycosyl hydrolases; ChtBD1 is a lectin domain found in proteins from plants and fungi that bind N-acetylglucosamine, plant endochitinases, wound-induced proteins, and the alpha subunit of Kluyveromyces lactis killer toxin. This domain is involved in the recognition and/or binding of chitin subunits; it typically occurs N-terminal to glycosyl hydrolase domains in chitinases, together with other carbohydrate-binding domains, or by itself in tandem-repeat arrangements.


Pssm-ID: 211313  Cd Length: 38  Bit Score: 39.33  E-value: 9.39e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
gi 6323242   28 SISCSPNNPCPAEwpCCSPYNECGAGP------ICVGGCN 61
Cdd:cd06922   1 PGTCSPTQPCKGG--CCNKDGVCGFGPdycgadVCISNCD 38
GH16_beta_GRP cd02179
beta-1,3-glucan recognition protein, member of glycosyl hydrolase family 16; Beta-GRP (beta-1, ...
 258-277 2.81e-03

beta-1,3-glucan recognition protein, member of glycosyl hydrolase family 16; Beta-GRP (beta-1,3-glucan recognition protein) is one of several pattern recognition receptors (PRRs), also referred to as biosensor proteins, that complexes with pathogen-associated beta-1,3-glucans and then transduces signals necessary for activation of an appropriate innate immune response. They are present in insects and lack all catalytic residues. This subgroup also contains related proteins of unknown function that still contain the active site. Their structures adopt a jelly roll fold with a deep active site channel harboring the catalytic residues, like those of other glycosyl hydrolase family 16 members.


Pssm-ID: 185688 [Multi-domain]  Cd Length: 321  Bit Score: 39.67  E-value: 2.81e-03
                        10        20
                ....*....|....*....|
gi 6323242  258 YHTYEIDWDPDRIIWYVDGK 277
Cdd:cd02179 202 FHVYTLEWKPDGITLMVDGE 221
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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