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Conserved domains on  [gi|22218619|ref|NP_056212|]
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DDB1- and CUL4-associated factor 12 [Homo sapiens]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 11455410)

WD40 repeat domain-containing protein similar to proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
PubMed:  10322433|8090199
SCOP:  4002744

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WD40 COG2319
WD40 repeat [General function prediction only];
101-283 9.15e-09

WD40 repeat [General function prediction only];


:

Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 57.23  E-value: 9.15e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 101 RQVVCGTKCNTLFVVDVQTSQITKIpilkdrepggVTQQGCGIHAIELNPSRTLLATGGDNpNSLAIYRLPTLDPVcVGD 180
Cdd:COG2319 259 RLLASGSADGTVRLWDLATGELLRT----------LTGHSGGVNSVAFSPDGKLLASGSDD-GTVRLWDLATGKLL-RTL 326

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 181 DGHKDWIFSIAWISD-TMAVSGSRDGSMGLWEVTDDVLTKSDARHnvsrvpvyahithkalkdipkedTNPdnckVRALA 259
Cdd:COG2319 327 TGHTGAVRSVAFSPDgKTLASGSDDGTVRLWDLATGELLRTLTGH-----------------------TGA----VTSVA 379

                       170       180
                ....*....|....*....|....
gi 22218619 260 FNNKNKELGAVSLDGYFHLWKAEN 283
Cdd:COG2319 380 FSPDGRTLASGSADGTVRLWDLAT 403
 
Name Accession Description Interval E-value
WD40 COG2319
WD40 repeat [General function prediction only];
101-283 9.15e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 57.23  E-value: 9.15e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 101 RQVVCGTKCNTLFVVDVQTSQITKIpilkdrepggVTQQGCGIHAIELNPSRTLLATGGDNpNSLAIYRLPTLDPVcVGD 180
Cdd:COG2319 259 RLLASGSADGTVRLWDLATGELLRT----------LTGHSGGVNSVAFSPDGKLLASGSDD-GTVRLWDLATGKLL-RTL 326

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 181 DGHKDWIFSIAWISD-TMAVSGSRDGSMGLWEVTDDVLTKSDARHnvsrvpvyahithkalkdipkedTNPdnckVRALA 259
Cdd:COG2319 327 TGHTGAVRSVAFSPDgKTLASGSDDGTVRLWDLATGELLRTLTGH-----------------------TGA----VTSVA 379

                       170       180
                ....*....|....*....|....
gi 22218619 260 FNNKNKELGAVSLDGYFHLWKAEN 283
Cdd:COG2319 380 FSPDGRTLASGSADGTVRLWDLAT 403
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 85-279 6.56e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 53.88  E-value: 6.56e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619  85 HLGTLNKVfaSQWLNHRQVVCGTKCNTLFVVDVQTSQITkipilkdREPGGVTQqgcGIHAIELNPSRTLLATGGDNpNS 164
Cdd:cd00200  50 HTGPVRDV--AASADGTYLASGSSDKTIRLWDLETGECV-------RTLTGHTS---YVSSVAFSPDGRILSSSSRD-KT 116

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 165 LAIYRLPTldPVCVGD-DGHKDWIFSIAWISD-TMAVSGSRDGSMGLWEVTddvltksdarhnvSRVPVYAHITHkalkd 242
Cdd:cd00200 117 IKVWDVET--GKCLTTlRGHTDWVNSVAFSPDgTFVASSSQDGTIKLWDLR-------------TGKCVATLTGH----- 176

                       170       180       190
                ....*....|....*....|....*....|....*..
gi 22218619 243 ipkedtnpdNCKVRALAFNNKNKELGAVSLDGYFHLW 279
Cdd:cd00200 177 ---------TGEVNSVAFSPDGEKLLSSSSDGTIKLW 204
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 181-211 6.30e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 34.60  E-value: 6.30e-03
                           10        20        30
                   ....*....|....*....|....*....|..
gi 22218619    181 DGHKDWIFSIAWISD-TMAVSGSRDGSMGLWE 211
Cdd:smart00320   9 KGHTGPVTSVAFSPDgKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
181-211 9.57e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 33.86  E-value: 9.57e-03
                          10        20        30
                  ....*....|....*....|....*....|..
gi 22218619   181 DGHKDWIFSIAWISD-TMAVSGSRDGSMGLWE 211
Cdd:pfam00400   8 EGHTGSVTSLAFSPDgKLLASGSDDGTVKVWD 39
 
Name Accession Description Interval E-value
WD40 COG2319
WD40 repeat [General function prediction only];
101-283 9.15e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 57.23  E-value: 9.15e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 101 RQVVCGTKCNTLFVVDVQTSQITKIpilkdrepggVTQQGCGIHAIELNPSRTLLATGGDNpNSLAIYRLPTLDPVcVGD 180
Cdd:COG2319 259 RLLASGSADGTVRLWDLATGELLRT----------LTGHSGGVNSVAFSPDGKLLASGSDD-GTVRLWDLATGKLL-RTL 326

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 181 DGHKDWIFSIAWISD-TMAVSGSRDGSMGLWEVTDDVLTKSDARHnvsrvpvyahithkalkdipkedTNPdnckVRALA 259
Cdd:COG2319 327 TGHTGAVRSVAFSPDgKTLASGSDDGTVRLWDLATGELLRTLTGH-----------------------TGA----VTSVA 379

                       170       180
                ....*....|....*....|....
gi 22218619 260 FNNKNKELGAVSLDGYFHLWKAEN 283
Cdd:COG2319 380 FSPDGRTLASGSADGTVRLWDLAT 403
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 85-279 6.56e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 53.88  E-value: 6.56e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619  85 HLGTLNKVfaSQWLNHRQVVCGTKCNTLFVVDVQTSQITkipilkdREPGGVTQqgcGIHAIELNPSRTLLATGGDNpNS 164
Cdd:cd00200  50 HTGPVRDV--AASADGTYLASGSSDKTIRLWDLETGECV-------RTLTGHTS---YVSSVAFSPDGRILSSSSRD-KT 116

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 165 LAIYRLPTldPVCVGD-DGHKDWIFSIAWISD-TMAVSGSRDGSMGLWEVTddvltksdarhnvSRVPVYAHITHkalkd 242
Cdd:cd00200 117 IKVWDVET--GKCLTTlRGHTDWVNSVAFSPDgTFVASSSQDGTIKLWDLR-------------TGKCVATLTGH----- 176

                       170       180       190
                ....*....|....*....|....*....|....*..
gi 22218619 243 ipkedtnpdNCKVRALAFNNKNKELGAVSLDGYFHLW 279
Cdd:cd00200 177 ---------TGEVNSVAFSPDGEKLLSSSSDGTIKLW 204
WD40 COG2319
WD40 repeat [General function prediction only];
142-282 3.85e-07

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 52.22  E-value: 3.85e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 142 GIHAIELNPSRTLLATGGDNpNSLAIYRLPTLDPVCVGDdGHKDWIFSIAWISD-TMAVSGSRDGSMGLWEVTDDVLTKS 220
Cdd:COG2319 164 AVTSVAFSPDGKLLASGSDD-GTVRLWDLATGKLLRTLT-GHTGAVRSVAFSPDgKLLASGSADGTVRLWDLATGKLLRT 241

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 22218619 221 DARHNVSrvpvyahithkalkdipkedtnpdnckVRALAFNNKNKELGAVSLDGYFHLWKAE 282
Cdd:COG2319 242 LTGHSGS---------------------------VRSVAFSPDGRLLASGSADGTVRLWDLA 276
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 85-280 3.90e-07

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 51.57  E-value: 3.90e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619  85 HLGTLNKVFASQwlNHRQVVCGTKCNTLFVVDVQTSQITKIpiLKDREpggvtqqgCGIHAIELNPSRTLLATGGDNpNS 164
Cdd:cd00200 134 HTDWVNSVAFSP--DGTFVASSSQDGTIKLWDLRTGKCVAT--LTGHT--------GEVNSVAFSPDGEKLLSSSSD-GT 200

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 165 LAIYRLPTLDPVCVGDdGHKDWIFSIAWISDT-MAVSGSRDGSMGLWEVTDDVLTKSDARHNVSrvpvyahithkalkdi 243
Cdd:cd00200 201 IKLWDLSTGKCLGTLR-GHENGVNSVAFSPDGyLLASGSEDGTIRVWDLRTGECVQTLSGHTNS---------------- 263

                       170       180       190
                ....*....|....*....|....*....|....*..
gi 22218619 244 pkedtnpdnckVRALAFNNKNKELGAVSLDGYFHLWK 280
Cdd:cd00200 264 -----------VTSLAWSPDGKRLASGSADGTIRIWD 289
WD40 COG2319
WD40 repeat [General function prediction only];
142-283 5.67e-07

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 51.45  E-value: 5.67e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 142 GIHAIELNPSRTLLATGGDNpNSLAIYRLPTLDPVCVGDdGHKDWIFSIAWISD-TMAVSGSRDGSMGLWEVTDDVLTKS 220
Cdd:COG2319 206 AVRSVAFSPDGKLLASGSAD-GTVRLWDLATGKLLRTLT-GHSGSVRSVAFSPDgRLLASGSADGTVRLWDLATGELLRT 283

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 22218619 221 DARHNVSrvpvyahithkalkdipkedtnpdnckVRALAFNNKNKELGAVSLDGYFHLWKAEN 283
Cdd:COG2319 284 LTGHSGG---------------------------VNSVAFSPDGKLLASGSDDGTVRLWDLAT 319
WD40 COG2319
WD40 repeat [General function prediction only];
101-283 7.33e-07

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 51.07  E-value: 7.33e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 101 RQVVCGTKCNTLFVVDVQTSQITKIpiLKDREPGgvtqqgcgIHAIELNPSRTLLATGGDNpNSLAIYRLPTLDPVCVGD 180
Cdd:COG2319 217 KLLASGSADGTVRLWDLATGKLLRT--LTGHSGS--------VRSVAFSPDGRLLASGSAD-GTVRLWDLATGELLRTLT 285

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 181 dGHKDWIFSIAWISD-TMAVSGSRDGSMGLWEVTDDVLTKSDARHNVSrvpvyahithkalkdipkedtnpdnckVRALA 259
Cdd:COG2319 286 -GHSGGVNSVAFSPDgKLLASGSDDGTVRLWDLATGKLLRTLTGHTGA---------------------------VRSVA 337

                       170       180
                ....*....|....*....|....
gi 22218619 260 FNNKNKELGAVSLDGYFHLWKAEN 283
Cdd:COG2319 338 FSPDGKTLASGSDDGTVRLWDLAT 361
WD40 COG2319
WD40 repeat [General function prediction only];
142-283 2.70e-06

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 49.52  E-value: 2.70e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 142 GIHAIELNPSRTLLATGGDNpNSLAIYRLPTLDPVCVGDdGHKDWIFSIAWISD-TMAVSGSRDGSMGLWEVTDDVLTKS 220
Cdd:COG2319 122 AVRSVAFSPDGKTLASGSAD-GTVRLWDLATGKLLRTLT-GHSGAVTSVAFSPDgKLLASGSDDGTVRLWDLATGKLLRT 199

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 22218619 221 DARHNVSrvpvyahithkalkdipkedtnpdnckVRALAFNNKNKELGAVSLDGYFHLWKAEN 283
Cdd:COG2319 200 LTGHTGA---------------------------VRSVAFSPDGKLLASGSADGTVRLWDLAT 235
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 130-220 1.03e-04

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 43.86  E-value: 1.03e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 130 DREPGGVTQQGCGiHAIELN-----PSRTLLATGGDNpNSLAIYRLPTldPVCVGD-DGHKDWIFSIAWISD-TMAVSGS 202
Cdd:cd00200  37 DLETGELLRTLKG-HTGPVRdvaasADGTYLASGSSD-KTIRLWDLET--GECVRTlTGHTSYVSSVAFSPDgRILSSSS 112

                        90
                ....*....|....*...
gi 22218619 203 RDGSMGLWEVTDDVLTKS 220
Cdd:cd00200 113 RDKTIKVWDVETGKCLTT 130
WD40 COG2319
WD40 repeat [General function prediction only];
142-283 1.89e-03

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 40.28  E-value: 1.89e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22218619 142 GIHAIELNPSRTLLATGGDnPNSLAIYRLPTLDPVCVGDdGHKDWIFSIAWISD-TMAVSGSRDGSMGLWEVTDDVLTKS 220
Cdd:COG2319  80 AVLSVAFSPDGRLLASASA-DGTVRLWDLATGLLLRTLT-GHTGAVRSVAFSPDgKTLASGSADGTVRLWDLATGKLLRT 157

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 22218619 221 DARHNVSrvpvyahithkalkdipkedtnpdnckVRALAFNNKNKELGAVSLDGYFHLWKAEN 283
Cdd:COG2319 158 LTGHSGA---------------------------VTSVAFSPDGKLLASGSDDGTVRLWDLAT 193
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 181-211 6.30e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 34.60  E-value: 6.30e-03
                           10        20        30
                   ....*....|....*....|....*....|..
gi 22218619    181 DGHKDWIFSIAWISD-TMAVSGSRDGSMGLWE 211
Cdd:smart00320   9 KGHTGPVTSVAFSPDgKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
181-211 9.57e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 33.86  E-value: 9.57e-03
                          10        20        30
                  ....*....|....*....|....*....|..
gi 22218619   181 DGHKDWIFSIAWISD-TMAVSGSRDGSMGLWE 211
Cdd:pfam00400   8 EGHTGSVTSLAFSPDgKLLASGSDDGTVKVWD 39
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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