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Conserved domains on  [gi|27229051|ref|NP_080543|]
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adaptin ear-binding coat-associated protein 1 [Mus musculus]

Protein Classification

adaptin-ear-binding coat-associated family protein( domain architecture ID 10546203)

adaptin-ear-binding coat-associated protein (NECAP) family protein is an alpha-ear-binding protein that enriches on clathrin-coated vesicles (CCVs)

CATH:  2.30.29.30
Gene Ontology:  GO:0006897
PubMed:  14555962
SCOP:  4001928

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DUF1681 pfam07933
Protein of unknown function (DUF1681); This family is composed of sequences derived from a ...
 7-164 5.54e-93

Protein of unknown function (DUF1681); This family is composed of sequences derived from a number of hypothetical eukaryotic proteins of unknown function.


:

Pssm-ID: 462320  Cd Length: 157  Bit Score: 271.30  E-value: 5.54e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27229051     7 YESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKIAYIKLEDKVSGELFAQAPVEqyPGIAVETVT 86
Cdd:pfam07933   1 IERVLFVAPEVHVYKIPPRTSNKGYRAADWTLANPIWTGRLRVVETGDKCDIRLEDPNTGELFAACPYP--GPAAVEPVL 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 27229051    87 DSSRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQETEISK-ESQEMDNRPKLDLGFKEGQTIKLSIG 164
Cdd:pfam07933  79 DSSRYFVLRVQDENGRKAFLGIGFEERSDAFDFNVALQDARKRLGREKEAEAgEAEAEEPKPPKDYSLKEGETITINIG 157
PRK14959 super family cl33044
DNA polymerase III subunits gamma and tau; Provisional
 161-275 5.62e-05

DNA polymerase III subunits gamma and tau; Provisional


The actual alignment was detected with superfamily member PRK14959:

Pssm-ID: 184923 [Multi-domain]  Cd Length: 624  Bit Score: 44.29  E-value: 5.62e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27229051  161 LSIGNITAKKGGASKPRASGTGGlsllpPPPGGKVTIPPP--------------SSSVAISNHVTP-------------P 213
Cdd:PRK14959 366 MPVESLRPSGGGASAPSGSAAEG-----PASGGAATIPTPgtqgpqgtapaagmTPSSAAPATPAPsaapsprvpwddaP 440

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 27229051  214 PIPKSNHGGNDSDILLDLDSPAPvstSAPAPVSTSNDLWGDFSTASSSVPNQAPQPSNWVQF 275
Cdd:PRK14959 441 PAPPRSGIPPRPAPRMPEASPVP---GAPDSVASASDAPPTLGDPSDTAEHTPSGPRTWDGF 499
 
Name Accession Description Interval E-value
DUF1681 pfam07933
Protein of unknown function (DUF1681); This family is composed of sequences derived from a ...
 7-164 5.54e-93

Protein of unknown function (DUF1681); This family is composed of sequences derived from a number of hypothetical eukaryotic proteins of unknown function.


Pssm-ID: 462320  Cd Length: 157  Bit Score: 271.30  E-value: 5.54e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27229051     7 YESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKIAYIKLEDKVSGELFAQAPVEqyPGIAVETVT 86
Cdd:pfam07933   1 IERVLFVAPEVHVYKIPPRTSNKGYRAADWTLANPIWTGRLRVVETGDKCDIRLEDPNTGELFAACPYP--GPAAVEPVL 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 27229051    87 DSSRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQETEISK-ESQEMDNRPKLDLGFKEGQTIKLSIG 164
Cdd:pfam07933  79 DSSRYFVLRVQDENGRKAFLGIGFEERSDAFDFNVALQDARKRLGREKEAEAgEAEAEEPKPPKDYSLKEGETITINIG 157
PHear_NECAP cd13228
NECAP (adaptin-ear-binding coat-associated protein) Plextrin Homology (PH) fold with ear-like ...
 9-128 1.39e-81

NECAP (adaptin-ear-binding coat-associated protein) Plextrin Homology (PH) fold with ear-like function (PHear) domain; NECAPs are alpha-ear-binding proteins that enrich on clathrin-coated vesicles (CCVs). NECAP 1 is expressed in brain and non-neuronal tissues and cells while NECAP 2 is ubiquitously expressed. The PH-like domain of NECAPs is a protein-binding interface that mimics the FxDxF motif binding properties of the alpha-ear and is called PHear (PH fold with ear-like function) domain. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270048  Cd Length: 120  Bit Score: 240.92  E-value: 1.39e-81
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27229051   9 SVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKIAYIKLEDKVSGELFAQAPVEQYPGIAVETVTDS 88
Cdd:cd13228   1 SVLLVKPEVYVYRIPPRASAAGYRAADWGLEKPIWTGRLRVVAKGEECEIRLEDPNTGELFAQCPVDVDPGTAVEPVLDS 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|
gi 27229051  89 SRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFK 128
Cdd:cd13228  81 SRYFVLRVEDGAGRHAFIGIGFRERSDAFDFNVALQDHFK 120
PRK14959 PRK14959
DNA polymerase III subunits gamma and tau; Provisional
 161-275 5.62e-05

DNA polymerase III subunits gamma and tau; Provisional


Pssm-ID: 184923 [Multi-domain]  Cd Length: 624  Bit Score: 44.29  E-value: 5.62e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27229051  161 LSIGNITAKKGGASKPRASGTGGlsllpPPPGGKVTIPPP--------------SSSVAISNHVTP-------------P 213
Cdd:PRK14959 366 MPVESLRPSGGGASAPSGSAAEG-----PASGGAATIPTPgtqgpqgtapaagmTPSSAAPATPAPsaapsprvpwddaP 440

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 27229051  214 PIPKSNHGGNDSDILLDLDSPAPvstSAPAPVSTSNDLWGDFSTASSSVPNQAPQPSNWVQF 275
Cdd:PRK14959 441 PAPPRSGIPPRPAPRMPEASPVP---GAPDSVASASDAPPTLGDPSDTAEHTPSGPRTWDGF 499
 
Name Accession Description Interval E-value
DUF1681 pfam07933
Protein of unknown function (DUF1681); This family is composed of sequences derived from a ...
 7-164 5.54e-93

Protein of unknown function (DUF1681); This family is composed of sequences derived from a number of hypothetical eukaryotic proteins of unknown function.


Pssm-ID: 462320  Cd Length: 157  Bit Score: 271.30  E-value: 5.54e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27229051     7 YESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKIAYIKLEDKVSGELFAQAPVEqyPGIAVETVT 86
Cdd:pfam07933   1 IERVLFVAPEVHVYKIPPRTSNKGYRAADWTLANPIWTGRLRVVETGDKCDIRLEDPNTGELFAACPYP--GPAAVEPVL 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 27229051    87 DSSRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQETEISK-ESQEMDNRPKLDLGFKEGQTIKLSIG 164
Cdd:pfam07933  79 DSSRYFVLRVQDENGRKAFLGIGFEERSDAFDFNVALQDARKRLGREKEAEAgEAEAEEPKPPKDYSLKEGETITINIG 157
PHear_NECAP cd13228
NECAP (adaptin-ear-binding coat-associated protein) Plextrin Homology (PH) fold with ear-like ...
 9-128 1.39e-81

NECAP (adaptin-ear-binding coat-associated protein) Plextrin Homology (PH) fold with ear-like function (PHear) domain; NECAPs are alpha-ear-binding proteins that enrich on clathrin-coated vesicles (CCVs). NECAP 1 is expressed in brain and non-neuronal tissues and cells while NECAP 2 is ubiquitously expressed. The PH-like domain of NECAPs is a protein-binding interface that mimics the FxDxF motif binding properties of the alpha-ear and is called PHear (PH fold with ear-like function) domain. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270048  Cd Length: 120  Bit Score: 240.92  E-value: 1.39e-81
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27229051   9 SVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKIAYIKLEDKVSGELFAQAPVEQYPGIAVETVTDS 88
Cdd:cd13228   1 SVLLVKPEVYVYRIPPRASAAGYRAADWGLEKPIWTGRLRVVAKGEECEIRLEDPNTGELFAQCPVDVDPGTAVEPVLDS 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|
gi 27229051  89 SRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFK 128
Cdd:cd13228  81 SRYFVLRVEDGAGRHAFIGIGFRERSDAFDFNVALQDHFK 120
PH-like cd00900
Pleckstrin homology-like domain; The PH-like family includes the PH domain, both the Shc-like ...
 39-116 1.08e-05

Pleckstrin homology-like domain; The PH-like family includes the PH domain, both the Shc-like and IRS-like PTB domains, the ran-binding domain, the EVH1 domain, a domain in neurobeachin and the third domain of FERM. All of these domains have a PH fold, but lack significant sequence similarity. They are generally involved in targeting to protein to the appropriate cellular location or interacting with a binding partner. This domain family possesses multiple functions including the ability to bind inositol phosphates and to other proteins.


Pssm-ID: 275390  Cd Length: 89  Bit Score: 43.16  E-value: 1.08e-05
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 27229051  39 DQPDWTGRLRITSkgkiAYIKLEDKVSGELFAQAPVEQYPGIAVETVTDSSRYFVIRIQDgtgRSAFIGIGFTDRGDA 116
Cdd:cd00900  12 PTKRVEGTLYITS----DRLILRDKNDGGLELSIPISDIVNVNVSPQGPSSRYLVLVLKD---RGEFVGFSFPKEEDA 82
PRK14959 PRK14959
DNA polymerase III subunits gamma and tau; Provisional
 161-275 5.62e-05

DNA polymerase III subunits gamma and tau; Provisional


Pssm-ID: 184923 [Multi-domain]  Cd Length: 624  Bit Score: 44.29  E-value: 5.62e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27229051  161 LSIGNITAKKGGASKPRASGTGGlsllpPPPGGKVTIPPP--------------SSSVAISNHVTP-------------P 213
Cdd:PRK14959 366 MPVESLRPSGGGASAPSGSAAEG-----PASGGAATIPTPgtqgpqgtapaagmTPSSAAPATPAPsaapsprvpwddaP 440

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 27229051  214 PIPKSNHGGNDSDILLDLDSPAPvstSAPAPVSTSNDLWGDFSTASSSVPNQAPQPSNWVQF 275
Cdd:PRK14959 441 PAPPRSGIPPRPAPRMPEASPVP---GAPDSVASASDAPPTLGDPSDTAEHTPSGPRTWDGF 499
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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