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Conserved domains on  [gi|13386024|ref|NP_080803|]
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putative glutathione-specific gamma-glutamylcyclotransferase 2 isoform 1 [Mus musculus]

Protein Classification

glutathione-specific gamma-glutamylcyclotransferase( domain architecture ID 10520616)

glutathione-specific gamma-glutamylcyclotransferase (gamma-glu-GCG or gamma-GCG) catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide; acts specifically on glutathione, but not on other gamma-glutamyl peptides

CATH:  3.10.490.10
EC:  4.3.2.7
Gene Ontology:  GO:0016746
PubMed:  18515354|23070364
SCOP:  4002485

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 1-174 1.61e-102

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


:

Pssm-ID: 398428  Cd Length: 176  Bit Score: 291.99  E-value: 1.61e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024     1 MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSVWGVAYKLPVGKEEEVKTYLD 80
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDEEGRTWGVAYRIPAETAEEVLEYLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024    81 FREK-GGYRTTTVIFYPK-DSTTKPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPE 158
Cdd:pfam04752  81 VREQiNGYTTHEVPFYPDqDTDGRPVNALVYVATPKNPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQALRKLGPG 160

                         170
                  ....*....|....*.
gi 13386024   159 DADEHLFSLEKLVKER 174
Cdd:pfam04752 161 VQDDHLFELEKLVKKL 176
 
Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 1-174 1.61e-102

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


Pssm-ID: 398428  Cd Length: 176  Bit Score: 291.99  E-value: 1.61e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024     1 MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSVWGVAYKLPVGKEEEVKTYLD 80
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDEEGRTWGVAYRIPAETAEEVLEYLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024    81 FREK-GGYRTTTVIFYPK-DSTTKPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPE 158
Cdd:pfam04752  81 VREQiNGYTTHEVPFYPDqDTDGRPVNALVYVATPKNPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQALRKLGPG 160

                         170
                  ....*....|....*.
gi 13386024   159 DADEHLFSLEKLVKER 174
Cdd:pfam04752 161 VQDDHLFELEKLVKKL 176
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 1-177 1.78e-58

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


Pssm-ID: 442917  Cd Length: 190  Bit Score: 180.78  E-value: 1.78e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024   1 MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLveDPGGSVWGVAYKLPVGKEEEVKTYLD 80
Cdd:COG3703  16 LWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGL--DRGGSCRGVAFRVPPEDAEAVLAYLW 93

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024  81 FREK--GGY--RTTTVIFypKDSTtkPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLv 156
Cdd:COG3703  94 RREMvtGVYlpRWLPVRL--ADGR--RVEALAFVADRDHPQYAGGLSLEEQARIIAQAVGPSGSNADYLLNTAEHLREL- 168

                       170       180
                ....*....|....*....|.
gi 13386024 157 pEDADEHLFSLEKLVKERLEG 177
Cdd:COG3703 169 -GIRDPHLEALARLVRALLAA 188
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 3-111 6.23e-13

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 61.59  E-value: 6.23e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024   3 VFGYGSLIWKVDF-----PYQDKLVGYITNYSRRFWQGStdhrgvpgkpgRVVTLVEDPGGSVWGVAYKLPvgkeEEVKT 77
Cdd:cd06661   1 LFVYGTLMDGEVLharlgRALFLGPATLKGYRLVFGGGS-----------GYPGLVPGPGARVWGELYEVD----PEDLA 65

                        90       100       110
                ....*....|....*....|....*....|....*.
gi 13386024  78 YLDFREK--GGYRTTTVIFYPKDSTTKPfsVLLYIG 111
Cdd:cd06661  66 RLDAFEGvpGGYRREEVEVELEDGEGVE--AWVYVA 99
 
Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 1-174 1.61e-102

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


Pssm-ID: 398428  Cd Length: 176  Bit Score: 291.99  E-value: 1.61e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024     1 MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSVWGVAYKLPVGKEEEVKTYLD 80
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDEEGRTWGVAYRIPAETAEEVLEYLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024    81 FREK-GGYRTTTVIFYPK-DSTTKPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPE 158
Cdd:pfam04752  81 VREQiNGYTTHEVPFYPDqDTDGRPVNALVYVATPKNPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQALRKLGPG 160

                         170
                  ....*....|....*.
gi 13386024   159 DADEHLFSLEKLVKER 174
Cdd:pfam04752 161 VQDDHLFELEKLVKKL 176
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 1-177 1.78e-58

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


Pssm-ID: 442917  Cd Length: 190  Bit Score: 180.78  E-value: 1.78e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024   1 MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLveDPGGSVWGVAYKLPVGKEEEVKTYLD 80
Cdd:COG3703  16 LWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGL--DRGGSCRGVAFRVPPEDAEAVLAYLW 93

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024  81 FREK--GGY--RTTTVIFypKDSTtkPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLv 156
Cdd:COG3703  94 RREMvtGVYlpRWLPVRL--ADGR--RVEALAFVADRDHPQYAGGLSLEEQARIIAQAVGPSGSNADYLLNTAEHLREL- 168

                       170       180
                ....*....|....*....|.
gi 13386024 157 pEDADEHLFSLEKLVKERLEG 177
Cdd:COG3703 169 -GIRDPHLEALARLVRALLAA 188
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 3-111 6.23e-13

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 61.59  E-value: 6.23e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386024   3 VFGYGSLIWKVDF-----PYQDKLVGYITNYSRRFWQGStdhrgvpgkpgRVVTLVEDPGGSVWGVAYKLPvgkeEEVKT 77
Cdd:cd06661   1 LFVYGTLMDGEVLharlgRALFLGPATLKGYRLVFGGGS-----------GYPGLVPGPGARVWGELYEVD----PEDLA 65

                        90       100       110
                ....*....|....*....|....*....|....*.
gi 13386024  78 YLDFREK--GGYRTTTVIFYPKDSTTKPfsVLLYIG 111
Cdd:cd06661  66 RLDAFEGvpGGYRREEVEVELEDGEGVE--AWVYVA 99
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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