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Conserved domains on  [gi|28178838|ref|NP_777358|]
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isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform b precursor [Homo sapiens]

Protein Classification

isocitrate/isopropylmalate dehydrogenase family protein( domain architecture ID 296)

isocitrate/isopropylmalate dehydrogenase family protein such as isocitrate dehydrogenase that in the Krebs cycle catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2, and isopropylmalate dehydrogenase that in leucine biosynthesis catalyzes the oxidation and decarboxylation of 3-isopropyl-l-malate to 4-methyl-2-oxovalerate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Iso_dh super family cl00445
Isocitrate/isopropylmalate dehydrogenase;
52-364 0e+00

Isocitrate/isopropylmalate dehydrogenase;


The actual alignment was detected with superfamily member TIGR00175:

Pssm-ID: 444908  Cd Length: 333  Bit Score: 562.39  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838    52 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 129
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   130 SRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 209
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   210 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 289
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 28178838   290 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENVRFP 364
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTK 314
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 52-364 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 562.39  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838    52 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 129
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   130 SRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 209
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   210 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 289
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 28178838   290 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENVRFP 364
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTK 314
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 47-364 3.80e-121

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 354.56  E-value: 3.80e-121
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   47 PSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRRNRVALKGNIETNHNLP 125
Cdd:PLN00123  23 PRPGDGAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRRNKVCLKGGLATPVGGG 100

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  126 PShkSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAF 205
Cdd:PLN00123 101 VS--SLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERIAKYAF 178

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  206 KLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCA 285
Cdd:PLN00123 179 EYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAA 258

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  286 GLVGGPGLVAGANYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENVR 362
Cdd:PLN00123 259 GIAGGTGVMPGGNVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGKYR 338


                 ..
gi 28178838  363 FP 364
Cdd:PLN00123 339 TK 340
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 54-362 3.30e-110

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 326.20  E-value: 3.30e-110
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  54 RHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETN 121
Cdd:COG0473   1 TYKIAVLPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGP 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838 122 HnlPPSHKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHK-----DIDILIVRENTEGEYSSLEHESVAG----VVESL 189
Cdd:COG0473  77 K--WDDGVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDT 154

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838 190 KIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDV 269
Cdd:COG0473 155 RVYTRKGIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDV 233

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838 270 MVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHLKLHSY 344
Cdd:COG0473 234 IVTENLFGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHLGEEEA 308

                       330
                ....*....|....*...
gi 28178838 345 ATSIRKAVLASMDnENVR 362
Cdd:COG0473 309 ADAIEAAVEKVLA-EGVR 325
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
56-361 1.06e-86

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 266.08  E-value: 1.06e-86
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838    56 TVTMIPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPS 127
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   128 HKSRNNI--LRTSLDLYANVIHCKSLPGVVTRHK------DIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAK 196
Cdd:pfam00180  81 VRPENGLlaLRKELGLFANLRPAKVFPPLGDASPlkneveGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   197 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLY 276
Cdd:pfam00180 161 IERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   277 GNIVNNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKAVLA 354
Cdd:pfam00180 241 GDILSDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLK 319


                  ....*..
gi 28178838   355 SMDNENV 361
Cdd:pfam00180 320 VLESGIR 326
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 52-364 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 562.39  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838    52 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 129
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   130 SRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 209
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   210 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 289
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 28178838   290 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENVRFP 364
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTK 314
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 47-364 3.80e-121

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 354.56  E-value: 3.80e-121
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   47 PSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRRNRVALKGNIETNHNLP 125
Cdd:PLN00123  23 PRPGDGAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRRNKVCLKGGLATPVGGG 100

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  126 PShkSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAF 205
Cdd:PLN00123 101 VS--SLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERIAKYAF 178

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  206 KLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCA 285
Cdd:PLN00123 179 EYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAA 258

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  286 GLVGGPGLVAGANYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENVR 362
Cdd:PLN00123 259 GIAGGTGVMPGGNVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGKYR 338


                 ..
gi 28178838  363 FP 364
Cdd:PLN00123 339 TK 340
PLN00118 PLN00118
isocitrate dehydrogenase (NAD+)
 20-362 2.30e-119

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215062  Cd Length: 372  Bit Score: 350.72  E-value: 2.30e-119
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   20 ALLCRPWEVLGAHEVPSRNIFSEQTIPPSAK----YGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNA 95
Cdd:PLN00118   3 AQLLRRLLGNRLAQILGASSSSSGAFSSSARafssSSTPITATLFPGDGIGPEIAESVKQVFTAAGVPIEWEEHYVGTTV 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   96 DEED----IRNAIMAIRRNRVALKGNIETNhnLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTE 171
Cdd:PLN00118  83 DPRTgsflTWESLESVRRNKVGLKGPMATP--IGKGHRSLNLTLRKELGLYANVRPCYSLPGYKTRYDDVDLVTIRENTE 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  172 GEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENM 251
Cdd:PLN00118 161 GEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRKRVSAIHKANIMKKTDGLFLKCCREVAEKYPEIVYEEV 240

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  252 IVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLA 331
Cdd:PLN00118 241 IIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGENGLALAEAVHGSAPDIAGKNLANPTALLLS 320

                        330       340       350
                 ....*....|....*....|....*....|.
gi 28178838  332 SCMMLDHLKLHSYATSIRKAVLASMDNENVR 362
Cdd:PLN00118 321 AVMMLRHLKLNEQAEQIHNAILNTIAEGKYR 351
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 54-362 3.30e-110

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 326.20  E-value: 3.30e-110
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  54 RHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETN 121
Cdd:COG0473   1 TYKIAVLPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGP 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838 122 HnlPPSHKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHK-----DIDILIVRENTEGEYSSLEHESVAG----VVESL 189
Cdd:COG0473  77 K--WDDGVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDT 154

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838 190 KIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDV 269
Cdd:COG0473 155 RVYTRKGIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDV 233

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838 270 MVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHLKLHSY 344
Cdd:COG0473 234 IVTENLFGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHLGEEEA 308

                       330
                ....*....|....*...
gi 28178838 345 ATSIRKAVLASMDnENVR 362
Cdd:COG0473 309 ADAIEAAVEKVLA-EGVR 325
PRK08997 PRK08997
isocitrate dehydrogenase; Provisional
 56-357 5.45e-102

isocitrate dehydrogenase; Provisional


Pssm-ID: 181606  Cd Length: 334  Bit Score: 304.72  E-value: 5.45e-102
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   56 TVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAIRRNRVALKGNIETNhnLPPSHKSR 131
Cdd:PRK08997   4 TITVIPGDGIGPSIIDATLKILDKLGCDFEYEFADAGLTALEKHGellpQRTLDLIEKNKIALKGPLTTP--VGEGFTSI 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  132 NNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHE-SVAG-VVESLKIITKAKSLRIAEYAFKLAQ 209
Cdd:PRK08997  82 NVTLRKKFDLYANVRPVLSFPGTKARYDNIDIITVRENTEGMYSGEGQTvSEDGeTAEATSIITRKGAERIVRFAYELAR 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  210 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 289
Cdd:PRK08997 162 KEGRKKVTAVHKANIMKSTSGLFLKVAREVALRYPDIEFEEMIVDATCMQLVMNPEQFDVIVTTNLFGDILSDLCAGLVG 241

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 28178838  290 GPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMD 357
Cdd:PRK08997 242 GLGMAPGANIGRDAAIFE-AVHGSAPDIAGKNLANPTSVILAAIQMLEYLGMPDKAERIRKAIVAVIE 308
PRK09222 PRK09222
NADP-dependent isocitrate dehydrogenase;
57-353 5.15e-98

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 236416 [Multi-domain]  Cd Length: 482  Bit Score: 299.50  E-value: 5.15e-98
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   57 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETnhnlpP---SH 128
Cdd:PRK09222   7 ITVAYGDGIGPEIMEAVLKILEAAGAPLEIETIEIGEKVYKKGWTSGISpsaweSIRRTKVLLKAPITT-----PqggGY 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  129 KSRNNILRTSLDLYANVIHCKSL-PGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKL 207
Cdd:PRK09222  82 KSLNVTLRKTLGLYANVRPCVSYhPFVETKHPNLDVVIIRENEEDLYAGIEHRQTPDVYQCLKLISRPGSEKIIRYAFEY 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  208 AQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 287
Cdd:PRK09222 162 ARANGRKKVTCLTKDNIMKLTDGLFHKVFDEIAKEYPDIEAEHYIVDIGAARLATNPENFDVIVTPNLYGDILSDIAAEI 241

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 28178838  288 VGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVL 353
Cdd:PRK09222 242 SGSVGLAGSANIGEEYAMFE-AVHGSAPDIAGKNIANPSGLLNAAVMMLVHIGQFDIAELIENAWL 306
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
56-361 1.06e-86

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 266.08  E-value: 1.06e-86
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838    56 TVTMIPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPS 127
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   128 HKSRNNI--LRTSLDLYANVIHCKSLPGVVTRHK------DIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAK 196
Cdd:pfam00180  81 VRPENGLlaLRKELGLFANLRPAKVFPPLGDASPlkneveGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   197 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLY 276
Cdd:pfam00180 161 IERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   277 GNIVNNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKAVLA 354
Cdd:pfam00180 241 GDILSDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLK 319


                  ....*..
gi 28178838   355 SMDNENV 361
Cdd:pfam00180 320 VLESGIR 326
PRK14025 PRK14025
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional
 55-352 4.08e-77

multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional


Pssm-ID: 184462  Cd Length: 330  Bit Score: 241.19  E-value: 4.08e-77
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   55 HTVTMIPGDGIGPELMLHVKSVFRhaCVPVDFEEVHVSSnADEEDIR-------NAIMAIRRNRVALKGNIETnhnlppS 127
Cdd:PRK14025   2 HKICVIEGDGIGKEVVPAALHVLE--ATGLPFEFVYAEA-GDEVFEKtgkalpeETIEAAKEADAVLFGAAGE------T 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  128 HKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKL 207
Cdd:PRK14025  73 AADVIVKLRRILDTYANVRPVKSYKGVKCLYPDIDYVIVRENTEGLYKGIEAEIADGVTVATRVITRKASERIFRFAFEM 152

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  208 AQ----ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNV 283
Cdd:PRK14025 153 AKrrkkMGKEGKVTCAHKANVLKKTDGLFKKTFYEVAKEYPDIKAEDYYVDAMNMYIITRPQTFDVVVTSNLFGDILSDG 232

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 28178838  284 CAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAV 352
Cdd:PRK14025 233 AAGLVGGLGLAPSANIGDKYGLFE-PVHGSAPDIAGKGIANPTATILTAVLMLRHLGENEEADKVEKAL 300
ICDH_alpha TIGR02924
isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of ...
 57-358 5.13e-77

isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of the isocitrate/isopropylmalate dehydrogenase superfamily described by pfam00180. Every member of the seed of this model appears to have a TCA cycle lacking only a determined isocitrate dehydrogenase. The precise identity of the cofactor (NADH -- 1.1.1.41 vs. NADPH -- 1.1.1.42) is unclear. [Energy metabolism, TCA cycle]


Pssm-ID: 274353 [Multi-domain]  Cd Length: 473  Bit Score: 245.44  E-value: 5.13e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838    57 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETNHNlpPSHKSR 131
Cdd:TIGR02924   3 ITVAYGDGIGPEIMEAVLLILKEAEAPIDIETIEIGEKVYKKGWPSGISpssweSIRRTKVLLKAPITTPQG--GGHKSL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   132 NNILRTSLDLYANVIHCKSL-PGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQE 210
Cdd:TIGR02924  81 NVTLRKTLGLYANIRPCVSYhPFIETKSPNLNIVIVRENEEDLYTGIEYRQTPDTYECTKLITRSGSEKICRYAFEYARK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   211 SGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGG 290
Cdd:TIGR02924 161 HNRKKVTCLTKDNIMKMTDGIFHKIFDKIAAEYPDIESEHYIVDIGMARLATNPENFDVIVTPNLYGDILSDVAAEISGS 240

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 28178838   291 PGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDN 358
Cdd:TIGR02924 241 VGLAGSANIGEEYAMFE-AVHGSAPDIAGQNIANPSGLLNAAIQMLVHIGQSDIAQLIYNAWLKTLED 307
LEU3_arch TIGR02088
isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal ...
 57-352 2.36e-74

isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal decarboxylating dehydrogenases which include the leucine biosynthesis enzyme 3-isopropylmalate dehydrogenase (LeuB, LEU3) and the methanogenic cofactor CoB biosynthesis enzyme isohomocitrate dehydrogenase (AksF). Both of these have been characterized in Methanococcus janaschii. Non-methanogenic archaea have only one hit to this model and presumably this is LeuB, although phylogenetic trees cannot establish which gene is which in the methanogens. The AksF gene is capable of acting on isohomocitrate, iso(homo)2-citrate and iso(homo)3-citrate in the successive elongation cycles of coenzyme B (7-mercaptoheptanoyl-threonine phosphate). This family is closely related to both the LeuB genes found in TIGR00169 and the mitochondrial eukaryotic isocitrate dehydratases found in TIGR00175. All of these are included within the broader subfamily model, pfam00180.


Pssm-ID: 273962  Cd Length: 322  Bit Score: 233.89  E-value: 2.36e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838    57 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRnaimAIRRNRVALKGNIETNhnLPPSH 128
Cdd:TIGR02088   1 VAVIPGDGIGPEVIEAAIRILNKLGLEIEFIEFEagdealkkYGSALPEDTLE----EIRKADAILFGAVTTP--ANPGY 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   129 KSRNNILRTSLDLYANVIHCKSLPGVVTRH-KDIDILIVRENTEGEYSSLEhESVAGVVESLKIITKAKSLRIAEYAFKL 207
Cdd:TIGR02088  75 KSVIVTLRKELDLYANVRPAKSLPGIPDLYpNGKDIVIVRENTEGLYAGFE-FGFSDRAIAIRVITREGSERIARFAFNL 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   208 AQESGRKkVTAVHKANIMKLGDGLFLQCCREVAARYpQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 287
Cdd:TIGR02088 154 AKERNRK-VTCVHKANVLKGTDGLFREVCREIAKRY-GVEYRDMYVDSAAMNLVKDPWRFDVIVTTNMFGDILSDLASAL 231

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 28178838   288 VGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAV 352
Cdd:TIGR02088 232 AGSLGLAPSANIGDRKALFE-PVHGSAPDIAGKGIANPTAAILSVAMMLDYLGELEKGKLVWEAV 295
PRK00772 PRK00772
3-isopropylmalate dehydrogenase; Provisional
 55-357 5.64e-52

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 234832  Cd Length: 358  Bit Score: 176.83  E-value: 5.64e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   55 HTVTMIPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNA-DE--EDIRNAIMAIRRNRVA-LKGNIETNH--NL 124
Cdd:PRK00772   3 YKIAVLPGDGIGPEVMaeaVKVlDAVAEKFGFDFEFEEALVGGAAiDAhgVPLPEETLEACRAADAvLLGAVGGPKwdNL 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  125 PPSHKSRNNIL--RTSLDLYAN---------VIHCKSL-PGVVtrhKDIDILIVRENTEGEY-------SSLEHESVAgv 185
Cdd:PRK00772  83 PPDVRPERGLLalRKELGLFANlrpaklypgLADASPLkPEIV---AGLDILIVRELTGGIYfgeprgrEGLGGEERA-- 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  186 VESLkIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQ 265
Cdd:PRK00772 158 FDTM-VYTREEIERIARVAFELARKR-RKKVTSVDKANVLE-SSRLWREVVTEVAKEYPDVELSHMYVDNAAMQLVRNPK 234

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  266 QFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHV-YAVFETAtrntGKS---IANKNIANPTATLLASCMMLDH-LK 340
Cdd:PRK00772 235 QFDVIVTENLFGDILSDEAAMLTGSLGMLPSASLGESgPGLYEPI----HGSapdIAGKGIANPIATILSAAMMLRYsLG 310

                        330
                 ....*....|....*..
gi 28178838  341 LHSYATSIRKAVLASMD 357
Cdd:PRK00772 311 LEEAADAIEAAVEKVLA 327
PRK03437 PRK03437
3-isopropylmalate dehydrogenase; Provisional
 59-354 4.80e-45

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 179579  Cd Length: 344  Bit Score: 158.17  E-value: 4.80e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   59 MIPGDGIGPELMLHVKSVFRHACVP-VDFEEVH-------------VSSNADEEDIR--NAIM--AIRRNRVAlKGNIET 120
Cdd:PRK03437   9 VIPGDGIGPEVVAEALKVLDAVAAGgPGVETTEydlgarrylrtgeTLPDSVLAELRqhDAILlgAIGDPSVP-SGVLER 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  121 NHNLPpshksrnniLRTSLDLYANVIHCKSLPGVVTRHK---DIDILIVRENTEGEYSS--------LEHEsVAGVVEsl 189
Cdd:PRK03437  88 GLLLK---------LRFALDHYVNLRPSKLYPGVTSPLAgpgDIDFVVVREGTEGPYTGnggalrvgTPHE-VATEVS-- 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  190 kIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDV 269
Cdd:PRK03437 156 -VNTAFGVERVVRDAFERAQKRPRKHLTLVHKTNVLTFAGDLWQRTVDEVAAEYPDVTVDYQHVDAATIFMVTDPSRFDV 234

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  270 MVMPNLYGNIVNNVCAGLVGGPGLVAGANY---GHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 346
Cdd:PRK03437 235 IVTDNLFGDIITDLAAAVTGGIGLAASGNInptGTNPSMFE-PVHGSAPDIAGQGIADPTAAILSVALLLDHLGEEDAAA 313


                 ....*...
gi 28178838  347 SIRKAVLA 354
Cdd:PRK03437 314 RIEAAVEA 321
PRK06451 PRK06451
NADP-dependent isocitrate dehydrogenase;
60-361 6.08e-43

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 235803  Cd Length: 412  Bit Score: 154.22  E-value: 6.08e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   60 IPGDGIGPELMLHVKSVFRHACV-------PVDFEEVHVSSNADEED----IRNAIMAIRRNRVALKGNIETNhnLPPSH 128
Cdd:PRK06451  29 VEGDGIGPEITHAAMKVINKAVEkaygsdrEIKWVEVLAGDKAEKLTgnrfPKESEELIEKYRVLLKGPLETP--IGKGW 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  129 KSRNNILRTSLDLYANVIHCKSLPGVVTRHKD---IDILIVRENTEGEYSSLEH----------------------ESVA 183
Cdd:PRK06451 107 KSINVAIRLMLDLYANIRPVKYIPGIESPLKNpekIDLIIFRENTDDLYRGIEYpydseeakkirdflrkelgvevEDDT 186

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  184 GVveSLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAR--------------------- 242
Cdd:PRK06451 187 GI--GIKLISKFKTQRIARMAIKYAIDHKRKKVTIMHKGNVMKYTEGAFREWAYEVALKefrdyvvteeevtknyngvpp 264

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  243 YPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNI 322
Cdd:PRK06451 265 SGKVIINDRIADNMFQQIIIRPDEYDIILAPNVNGDYISDAAGALVGNIGMLGGANIGDTGGMFE-AIHGTAPKYAGKNV 343

                        330       340       350
                 ....*....|....*....|....*....|....*....
gi 28178838  323 ANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENV 361
Cdd:PRK06451 344 ANPTGIIKGGELMLRFMGWDKAADLIDKAIMESIKQKKV 382
leuB TIGR00169
3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including ...
 57-364 6.72e-41

3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates.Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115. Other contacts of importance are known from crystallography but not detailed here.This model will not find all isopropylmalate dehydrogenases; the enzyme from Sulfolobus sp. strain 7 is more similar to mitochondrial NAD-dependent isocitrate dehydrogenases than to other known isopropylmalate dehydrogenases and was omitted to improve the specificity of the model. It scores below the cutoff and below some enzymes known not to be isopropylmalate dehydrogenase. [Amino acid biosynthesis, Pyruvate family]


Pssm-ID: 272939  Cd Length: 346  Bit Score: 147.16  E-value: 6.72e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838    57 VTMIPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNADE---EDIRNAIMAIRRNRVA-LKGNIETNH--NLPP 126
Cdd:TIGR00169   2 IAVLPGDGIGPEVMaqaLKVlKAVAERFGLKFEFEEHLIGGAAIDatgQPLPEETLKGCKEADAvLLGAVGGPKwdNLPR 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   127 SHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRH-------KDIDILIVRENTEGEYSSLEHE-SVAGVVESLKIITKAK 196
Cdd:TIGR00169  82 DQRPEQGLLklRKSLDLFANLRPAKVFPGLEDLSplkeeiaKGVDFVVVRELTGGIYFGEPKGrEGEGEAWDTEVYTVPE 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   197 SLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDgLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLY 276
Cdd:TIGR00169 162 IERIARVAFEMARKR-RKKVTSVDKANVLESSR-LWRKTVEEIAKEYPDVELEHQYIDNAAMQLVKSPTQFDVVVTSNLF 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   277 GNIVNNVCAGLVGGPGLVAGANYGH-VYAVFETAtRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLAS 355
Cdd:TIGR00169 240 GDILSDEASVITGSLGMLPSASLGSdGFGLFEPV-HGSAPDIAGKGIANPIAQILSAAMLLRYSFNLEEAADAIEAAVKK 318


                  ....*....
gi 28178838   356 MDNENVRFP 364
Cdd:TIGR00169 319 VLAEGYRTP 327
Icd COG0538
Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part ...
 60-352 1.03e-40

Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440304  Cd Length: 409  Bit Score: 148.34  E-value: 1.03e-40
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  60 IPGDGIGPELMLHVKSVFRHA-------CVPVDFEEVHVS-SNADEEDIR---NAIMAIRRNRVALKGNIETnhnlpP-- 126
Cdd:COG0538  24 IEGDGIGPEITRAIWKVIDAAvekayggKRDIEWKEVDAGeKARDETGDWlpdETAEAIKEYGVGIKGPLTT-----Pvg 98

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838 127 -SHKSRNNILRTSLDLYAN---VIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHES----VAGVVESL--------- 189
Cdd:COG0538  99 gGWRSLNVTIRQILDLYVCrrpVRYFKGVPSPVKHPEKVDIVIFRENTEDIYAGIEWKAgspeALKLIFFLedemgvtvi 178

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838 190 ----------KIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAR-YP-------------- 244
Cdd:COG0538 179 rfpedsgigiKPVSDEGTERLVRAAIQYALDNKRKSVTLVHKGNIMKFTEGAFKDWGYEVAEEeFGdkfitegpwekykg 258

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838 245 -----QITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntg 314
Cdd:COG0538 259 pkpagKIVYKDRIADDMLQQILLRPGEYDVIATKNLNGDYISDALAAQVGGLGIAPGANIGdDGGAEFEathgTAPK--- 335

                       330       340       350
                ....*....|....*....|....*....|....*...
gi 28178838 315 ksIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAV 352
Cdd:COG0538 336 --YAGKDSTNPGSLILSGTMMLRHRGWLEAADLIEKAV 371
PRK08194 PRK08194
tartrate dehydrogenase; Provisional
 56-339 2.67e-38

tartrate dehydrogenase; Provisional


Pssm-ID: 181281  Cd Length: 352  Bit Score: 140.63  E-value: 2.67e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   56 TVTMIPGDGIGPELMLHVKSVFR-----HACVPVDFEEVHVSSnadEEDIRNAIMAIRRNRVALKgNIET-------NHN 123
Cdd:PRK08194   5 KIAVIPGDGVGKEVVPAAVRVLKavaevHGGLKFEFTEFPWSC---EYYLEHGEMMPEDGLEQLK-QFDAiflgavgNPK 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  124 LPPSHKSRNNIL---RTSLDLYANVIHCKSLPGV---VTRHKDIDILIVRENTEGEYSSL-------EHEsvagVVESLK 190
Cdd:PRK08194  81 LVPDHISLWGLLikiRREFEQVINIRPAKQLRGIkspLANPKDFDLLVVRENSEGEYSEVggrihrgEDE----IAIQNA 156

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  191 IITKAKSLRIAEYAFKLAQESgRKKVTAVHKAN----IMKLGDGLFlqccREVAARYPQITFENMIVDNTTMQLVSRPQQ 266
Cdd:PRK08194 157 VFTRKGTERAMRYAFELAAKR-RKHVTSATKSNgivhSMPFWDEVF----QEVGKDYPEIETDSQHIDALAAFFVTRPEE 231

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 28178838  267 FDVMVMPNLYGNIVNNVCAGLVGGPGLVAGAN---YGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHL 339
Cdd:PRK08194 232 FDVIVASNLFGDILTDIGAAIMGSIGIAPAANinvNGKYPSMFE-PVHGSAPDIAGKGIANPIGQIWTAKLMLDHF 306
PRK07006 PRK07006
isocitrate dehydrogenase; Reviewed
 106-361 7.46e-32

isocitrate dehydrogenase; Reviewed


Pssm-ID: 180792  Cd Length: 409  Bit Score: 124.40  E-value: 7.46e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  106 AIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYA---NVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEH 179
Cdd:PRK07006  84 LIREYRVAIKGPLTT-----PvggGIRSLNVALRQELDLYVclrPVRYFKGVPSPVKRPEDTDMVIFRENSEDIYAGIEW 158

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  180 EsvAGVVESLKII-------------------------TKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQ 234
Cdd:PRK07006 159 K--AGSAEAKKVIkflqeemgvkkirfpetsgigikpvSEEGTERLVRAAIEYAIDNDRKSVTLVHKGNIMKFTEGAFKD 236

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  235 CCREVAARY--------------------PQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLV 294
Cdd:PRK07006 237 WGYQLAEEEfgdelidggpwdkiknpetgKEIIVKDSIADAFLQQILLRPAEYDVIATMNLNGDYISDALAAQVGGIGIA 316

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 28178838  295 AGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENV 361
Cdd:PRK07006 317 PGANINDGHAIFE-ATHGTAPKYAGLDKVNPGSVILSAEMMLRHMGWTEAADLIIKSMEKTIASKTV 382
PLN02329 PLN02329
3-isopropylmalate dehydrogenase
 54-357 3.30e-25

3-isopropylmalate dehydrogenase


Pssm-ID: 215188  Cd Length: 409  Bit Score: 105.93  E-value: 3.30e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838   54 RHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNI------E 119
Cdd:PLN02329  46 RYNIALLPGDGIGPEVISVAKNVLQKAGslegLEFDFQEMPVGGAAlDLVGVplpEETFTAAKQSDAILLGAIggykwdK 125

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  120 TNHNLPPSHKSRNniLRTSLDLYANVIHCKSLPGVV---TRHKDI----DILIVRENTEGEYSSLE-----HESVAGVVE 187
Cdd:PLN02329 126 NEKHLRPEMALFY--LRRDLKVFANLRPATVLPQLVdasTLKKEVaegvDMMIVRELTGGIYFGEPrgitiNENGEEVGV 203

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  188 SLKIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQF 267
Cdd:PLN02329 204 STEIYAAHEIDRIARVAFETARKR-RGKLCSVDKANVLD-ASILWRKRVTALASEYPDVELSHMYVDNAAMQLIRDPKQF 281

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  268 DVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYAT 346
Cdd:PLN02329 282 DTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYgLGEEKAAK 361

                        330
                 ....*....|.
gi 28178838  347 SIRKAVLASMD 357
Cdd:PLN02329 362 RIEDAVVDALN 372
PRK07362 PRK07362
NADP-dependent isocitrate dehydrogenase;
104-378 1.81e-23

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 180944  Cd Length: 474  Bit Score: 101.33  E-value: 1.81e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  104 IMAIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYANVIHCKSLPGVVTRHK---DIDILIVRENTEGEYSSL 177
Cdd:PRK07362  91 LEAIREYGVAIKGPLTT-----PiggGIRSLNVALRQIFDLYSCVRPCRYYAGTPSPHKnpeKLDVIVYRENTEDIYMGI 165

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  178 EHESVAGVVESL----------------------------KIITKAKSLRIAEYAFK--LAQESGRKKVTAVHKANIMKL 227
Cdd:PRK07362 166 EWEAGDEIGDKLikhlneevipaspelgkrqiplgsgigiKPVSKTGSQRHIRRAIEhaLRLPGDKRHVTLVHKGNIMKY 245

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  228 GDGLFLQCCREVAA--------------------RYPQITFEN------------------------------------- 250
Cdd:PRK07362 246 TEGAFRDWGYELATtefrdecvtereswilsnkeKNPNISIEDnarmiepgydsltpekkaaicaevkevldsiwsshgn 325

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178838  251 -----------MIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIAN 319
Cdd:PRK07362 326 gkwkekvlvddRIADSIFQQIQTRPQEYSILATLNLNGDYISDAAAAIVGGLGMAPGANIGDNAAIFE-ATHGTAPKHAG 404

                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 28178838  320 KNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENVRFpSHPTLLPRPVSP--CS 378
Cdd:PRK07362 405 LDRINPGSVILSGVMMLEYLGWQEAADLITKGLSAAIANKQVTY-DLARLMEPPVDPlsCS 464
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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