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Conserved domains on  [gi|530388094|ref|XP_005273716|]
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DNA replication complex GINS protein SLD5 isoform X1 [Homo sapiens]

Protein Classification

DNA replication complex GINS protein SLD5( domain architecture ID 16826204)

DNA replication complex GINS protein SLD5 is a component of the heterotetrameric GINS complex; the GINS complex is essential for both the initiation and elongation stages of eukaryotic DNA replication

CATH:  3.40.5.60|1.20.58.1030
Gene Ontology:  GO:0006261
PubMed:  22918584|20070258

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GINS_A_Sld5 cd11711
Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric ...
 26-146 1.91e-48

Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric protein complex, and within the complex Sld5 interacts with Psf1 via its N-terminal A-domain, and with Psf2 through a combination of the A and B domains. Sld5 in Drosophila is required for normal cell cycle progression and the maintenance of genomic integrity. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


:

Pssm-ID: 212549  Cd Length: 119  Bit Score: 154.69  E-value: 1.91e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530388094  26 LIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAK---REDLKVSIHQMEMERIRYVLSSYLRCRLMKIEK 102
Cdd:cd11711    1 DLELLKRAWVNEKFAPELLPYQEELVERVLEQIEQQEENLEELKaseKDDLRLSLHEMELERIRFLLRSYLRTRLQKIEK 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 530388094 103 FFPHVLEKEKtrpegEPSSLSPEELAFAREFMANTESYLKNVAL 146
Cdd:cd11711   81 YALHILSEEE-----LPSRLSPEELEFAKRYAELLENHLKSSVL 119
GINS_B_Sld5 cd21692
beta-strand (B) domain of GINS complex protein Sld5; Sld5 is a component of the GINS (named ...
 165-223 8.96e-22

beta-strand (B) domain of GINS complex protein Sld5; Sld5 is a component of the GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) tetrameric protein complex, within which Sld5 interacts with Psf1 via its N-terminal A-domain, and with Psf2 through a combination of the A and B domains. In Drosophila, Sld5 is required for normal cell cycle progression and the maintenance of genomic integrity. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits Sld5, Psf1, Psf2 and Psf3 are homologous, and homologs are also found in archaea; the complex is not found in bacteria. Each subunit of the complex consists of two domains called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3. This model represents the B-domain of GINS subunit Sld5.


:

Pssm-ID: 412028  Cd Length: 55  Bit Score: 84.52  E-value: 8.96e-22
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 530388094 165 PDLDSYVFLRVRERQENILVEPDTDEqrdyVIDLEKGSQHLIRYKTIAPLVASGAVQLI 223
Cdd:cd21692    1 PNLDSFVFCRVLEDVGDVLVDPDGDE----EVDLEKGDIHLIRYRSIKDLVESGDVELI 55
 
Name Accession Description Interval E-value
GINS_A_Sld5 cd11711
Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric ...
 26-146 1.91e-48

Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric protein complex, and within the complex Sld5 interacts with Psf1 via its N-terminal A-domain, and with Psf2 through a combination of the A and B domains. Sld5 in Drosophila is required for normal cell cycle progression and the maintenance of genomic integrity. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212549  Cd Length: 119  Bit Score: 154.69  E-value: 1.91e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530388094  26 LIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAK---REDLKVSIHQMEMERIRYVLSSYLRCRLMKIEK 102
Cdd:cd11711    1 DLELLKRAWVNEKFAPELLPYQEELVERVLEQIEQQEENLEELKaseKDDLRLSLHEMELERIRFLLRSYLRTRLQKIEK 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 530388094 103 FFPHVLEKEKtrpegEPSSLSPEELAFAREFMANTESYLKNVAL 146
Cdd:cd11711   81 YALHILSEEE-----LPSRLSPEELEFAKRYAELLENHLKSSVL 119
GINS_B_Sld5 cd21692
beta-strand (B) domain of GINS complex protein Sld5; Sld5 is a component of the GINS (named ...
 165-223 8.96e-22

beta-strand (B) domain of GINS complex protein Sld5; Sld5 is a component of the GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) tetrameric protein complex, within which Sld5 interacts with Psf1 via its N-terminal A-domain, and with Psf2 through a combination of the A and B domains. In Drosophila, Sld5 is required for normal cell cycle progression and the maintenance of genomic integrity. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits Sld5, Psf1, Psf2 and Psf3 are homologous, and homologs are also found in archaea; the complex is not found in bacteria. Each subunit of the complex consists of two domains called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3. This model represents the B-domain of GINS subunit Sld5.


Pssm-ID: 412028  Cd Length: 55  Bit Score: 84.52  E-value: 8.96e-22
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 530388094 165 PDLDSYVFLRVRERQENILVEPDTDEqrdyVIDLEKGSQHLIRYKTIAPLVASGAVQLI 223
Cdd:cd21692    1 PNLDSFVFCRVLEDVGDVLVDPDGDE----EVDLEKGDIHLIRYRSIKDLVESGDVELI 55
SLD5_C pfam16922
DNA replication complex GINS protein SLD5 C-terminus; The C-terminal domain of DNA replication ...
 165-223 1.24e-19

DNA replication complex GINS protein SLD5 C-terminus; The C-terminal domain of DNA replication complex GINS protein SLD5 is important in the assembly of the GINS complex, a complex which is involved in initiation of DNA replication and progression of DNA replication forks.


Pssm-ID: 465315  Cd Length: 57  Bit Score: 78.73  E-value: 1.24e-19
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 530388094  165 PDLDSYVFLRVRERQENILVEPDTDEQRdyVIDLEKGSQHLIRYKTIAPLVASGAVQLI 223
Cdd:pfam16922   1 PDLDTFVFCRVLKDVGGVTVEGEGEDGD--VVELEKGDVYVLRYSSVKPLVESGKVELI 57
COG5086 COG5086
Uncharacterized conserved protein [Function unknown];
18-223 3.64e-17

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227418 [Multi-domain]  Cd Length: 218  Bit Score: 76.93  E-value: 3.64e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530388094  18 EVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVM----EQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYL 93
Cdd:COG5086   15 AVDSMSFPDLNDLILAWQNERSTPRLLPYALHLIDRFLdrisEQTEHVKIALGKSIAKNNESILMETELERVKFVVRSYI 94

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530388094  94 RCRLMKIEKFFPHVLEKEKTrpegePSSLSPEELAFAREFMANTESYLKNVALKHMPPNLQKVDLFRA----VPKPDLDS 169
Cdd:COG5086   95 RTRLKKIDKNLYYDLSDENS-----LILLSGGELAYYEKHSLIYIDLLKDSDLYVTPQKFHKVHDFVGfycmTDEPDWNK 169

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 530388094 170 YVFLRVRERQEnilvEPDTDEQRDYvIDLEKGSQHLIRYKTIAPLVASGAVQLI 223
Cdd:COG5086  170 FVFGAVNEDLQ----ENDTVGEKEL-LELFKGDFFVARYDVIMDFLRRNEVILI 218
Sld5 pfam05916
GINS complex protein; The eukaryotic GINS complex is essential for the initiation and ...
 49-127 4.62e-06

GINS complex protein; The eukaryotic GINS complex is essential for the initiation and elongation phases of DNA replication. It consists of four paralogous protein subunits (Sld5, Psf1, Psf2 and Psf3), all of which are included in this family. The GINS complex is conserved from yeast to humans, and has been shown in human to bind directly to DNA primase.


Pssm-ID: 399129  Cd Length: 105  Bit Score: 44.02  E-value: 4.62e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530388094   49 EIVECVMEQLEHMEENLRRAKREDLK--------VSIHQMEMER---IRYVLSSYLRCRLMKIEKFFPHVLEKEktRPEG 117
Cdd:pfam05916  18 EIEPPEWLSIEELEAILADETKNDLSllppyfyeLAVLLLELERddeLKRLLRDYLRIRLAKIRKLAWHLNGSL--SPSD 95

                          90
                  ....*....|
gi 530388094  118 EPSSLSPEEL 127
Cdd:pfam05916  96 LLDNLSEEEL 105
 
Name Accession Description Interval E-value
GINS_A_Sld5 cd11711
Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric ...
 26-146 1.91e-48

Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric protein complex, and within the complex Sld5 interacts with Psf1 via its N-terminal A-domain, and with Psf2 through a combination of the A and B domains. Sld5 in Drosophila is required for normal cell cycle progression and the maintenance of genomic integrity. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212549  Cd Length: 119  Bit Score: 154.69  E-value: 1.91e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530388094  26 LIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAK---REDLKVSIHQMEMERIRYVLSSYLRCRLMKIEK 102
Cdd:cd11711    1 DLELLKRAWVNEKFAPELLPYQEELVERVLEQIEQQEENLEELKaseKDDLRLSLHEMELERIRFLLRSYLRTRLQKIEK 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 530388094 103 FFPHVLEKEKtrpegEPSSLSPEELAFAREFMANTESYLKNVAL 146
Cdd:cd11711   81 YALHILSEEE-----LPSRLSPEELEFAKRYAELLENHLKSSVL 119
GINS_A cd11581
Alpha-helical domain of GINS complex proteins; Sld5, Psf1, Psf2 and Psf3; The GINS complex is ...
 27-139 1.29e-25

Alpha-helical domain of GINS complex proteins; Sld5, Psf1, Psf2 and Psf3; The GINS complex is involved in both initiation and elongation stages of eukaryotic chromosome replication, with GINS being the component that most likely serves as the replicative helicase that unwinds duplex DNA ahead of the moving replication fork. In eukaryotes, GINS is a tetrameric arrangement of four subunits Sld5, Psf1, Psf2 and Psf3. The GINS complex has been found in eukaryotes and archaea, but not in bacteria. The four subunits of the complex are homologous and consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212547  Cd Length: 103  Bit Score: 96.02  E-value: 1.29e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530388094  27 IERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYLRCRLMKIEKFFPH 106
Cdd:cd11581    1 VEKLAEAIHNERKSENLLPPPPDFMEEVKELLHRLSKRQRSETQEILEHRLKILELSYIRDLLKTYLNSRLDKIRDLSPE 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 530388094 107 VLekektrpegePSSLSPEELAFAREFMANTES 139
Cdd:cd11581   81 FL----------PSNLTEEEIGYFQTYWKEVAE 103
GINS_B_Sld5 cd21692
beta-strand (B) domain of GINS complex protein Sld5; Sld5 is a component of the GINS (named ...
 165-223 8.96e-22

beta-strand (B) domain of GINS complex protein Sld5; Sld5 is a component of the GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) tetrameric protein complex, within which Sld5 interacts with Psf1 via its N-terminal A-domain, and with Psf2 through a combination of the A and B domains. In Drosophila, Sld5 is required for normal cell cycle progression and the maintenance of genomic integrity. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits Sld5, Psf1, Psf2 and Psf3 are homologous, and homologs are also found in archaea; the complex is not found in bacteria. Each subunit of the complex consists of two domains called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3. This model represents the B-domain of GINS subunit Sld5.


Pssm-ID: 412028  Cd Length: 55  Bit Score: 84.52  E-value: 8.96e-22
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 530388094 165 PDLDSYVFLRVRERQENILVEPDTDEqrdyVIDLEKGSQHLIRYKTIAPLVASGAVQLI 223
Cdd:cd21692    1 PNLDSFVFCRVLEDVGDVLVDPDGDE----EVDLEKGDIHLIRYRSIKDLVESGDVELI 55
SLD5_C pfam16922
DNA replication complex GINS protein SLD5 C-terminus; The C-terminal domain of DNA replication ...
 165-223 1.24e-19

DNA replication complex GINS protein SLD5 C-terminus; The C-terminal domain of DNA replication complex GINS protein SLD5 is important in the assembly of the GINS complex, a complex which is involved in initiation of DNA replication and progression of DNA replication forks.


Pssm-ID: 465315  Cd Length: 57  Bit Score: 78.73  E-value: 1.24e-19
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 530388094  165 PDLDSYVFLRVRERQENILVEPDTDEQRdyVIDLEKGSQHLIRYKTIAPLVASGAVQLI 223
Cdd:pfam16922   1 PDLDTFVFCRVLKDVGGVTVEGEGEDGD--VVELEKGDVYVLRYSSVKPLVESGKVELI 57
COG5086 COG5086
Uncharacterized conserved protein [Function unknown];
18-223 3.64e-17

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227418 [Multi-domain]  Cd Length: 218  Bit Score: 76.93  E-value: 3.64e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530388094  18 EVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVM----EQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYL 93
Cdd:COG5086   15 AVDSMSFPDLNDLILAWQNERSTPRLLPYALHLIDRFLdrisEQTEHVKIALGKSIAKNNESILMETELERVKFVVRSYI 94

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530388094  94 RCRLMKIEKFFPHVLEKEKTrpegePSSLSPEELAFAREFMANTESYLKNVALKHMPPNLQKVDLFRA----VPKPDLDS 169
Cdd:COG5086   95 RTRLKKIDKNLYYDLSDENS-----LILLSGGELAYYEKHSLIYIDLLKDSDLYVTPQKFHKVHDFVGfycmTDEPDWNK 169

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 530388094 170 YVFLRVRERQEnilvEPDTDEQRDYvIDLEKGSQHLIRYKTIAPLVASGAVQLI 223
Cdd:COG5086  170 FVFGAVNEDLQ----ENDTVGEKEL-LELFKGDFFVARYDVIMDFLRRNEVILI 218
GINS_B cd21396
beta-strand (B) domain of GINS complex proteins: Sld5, Psf1, Psf2, Psf3, Gins51 and Gins23; ...
 170-223 7.27e-10

beta-strand (B) domain of GINS complex proteins: Sld5, Psf1, Psf2, Psf3, Gins51 and Gins23; The GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) complex is involved in both the initiation and elongation stages of eukaryotic chromosome replication, with GINS being the component that most likely serves as the replicative helicase that unwinds duplex DNA ahead of the moving replication fork. This complex is found in eukaryotes and archaea, but not in bacteria. In eukaryotes, GINS is a tetrameric arrangement of four subunits Sld5, Psf1, Psf2 and Psf3, while in archaea, it consists of two different proteins named Gins51 and Gins23. The archaeal GINS complex can be either an alpha2beta2-type heterotetramer composed of Gins51 and Gins23, or a Gins51-only alpha4-type homotetramer. All GINS subunits are homologous and consist of two domains, called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1/Gins51 are permuted with respect to Psf1/Psf3/Gins23. The overall tetrameric assemblies of GINS are similar, but the relative locations of the C-terminal small domains are different with respect to the alpha-helical domain, resulting in different subunit contacts. However, the basic function of GINS in DNA replication is conserved across eukaryotes and archaea. This model represents the beta-strand domain (B-domain) of GINS complex proteins.


Pssm-ID: 412027  Cd Length: 49  Bit Score: 52.85  E-value: 7.27e-10
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 530388094 170 YVFLRVRERQENILVEPdtdeqRDYVIDLEKGSQHLIRYKTIAPLVASGAVQLI 223
Cdd:cd21396    1 KVAVRVEKDLPRILAEF-----AETVGPFPKGSQVEVPLWLARGLVQRGALRLI 49
Sld5 pfam05916
GINS complex protein; The eukaryotic GINS complex is essential for the initiation and ...
 49-127 4.62e-06

GINS complex protein; The eukaryotic GINS complex is essential for the initiation and elongation phases of DNA replication. It consists of four paralogous protein subunits (Sld5, Psf1, Psf2 and Psf3), all of which are included in this family. The GINS complex is conserved from yeast to humans, and has been shown in human to bind directly to DNA primase.


Pssm-ID: 399129  Cd Length: 105  Bit Score: 44.02  E-value: 4.62e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530388094   49 EIVECVMEQLEHMEENLRRAKREDLK--------VSIHQMEMER---IRYVLSSYLRCRLMKIEKFFPHVLEKEktRPEG 117
Cdd:pfam05916  18 EIEPPEWLSIEELEAILADETKNDLSllppyfyeLAVLLLELERddeLKRLLRDYLRIRLAKIRKLAWHLNGSL--SPSD 95

                          90
                  ....*....|
gi 530388094  118 EPSSLSPEEL 127
Cdd:pfam05916  96 LLDNLSEEEL 105
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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