NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|578804490|ref|XP_006712657|]
View 

pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial isoform X6 [Homo sapiens]

Protein Classification

alpha-ketoacid dehydrogenase kinase family protein( domain architecture ID 13768662)

alpha-ketoacid dehydrogenase kinase family protein similar to branched-chain alpha-ketoacid dehydrogenase kinase (BCDHK), which is part of the mitochondrial protein kinases family and is a regulator of the valine, leucine, and isoleucine catabolic pathways; contains a C-terminal histidine kinase-like ATPase domain

CATH:  1.20.140.20|3.30.565.10
Gene Ontology:  GO:0004672|GO:0005524|GO:0016310
PubMed:  8253790|10339418|10637609
SCOP:  3000091

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
BCDHK_Adom3 pfam10436
Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase; Catabolism and synthesis of ...
 56-240 3.64e-62

Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase; Catabolism and synthesis of leucine, isoleucine and valine are finely balanced, allowing the body to make the most of dietary input but removing excesses to prevent toxic build-up of their corresponding keto-acids. This is the butyryl-CoA dehydrogenase, subunit A domain 3, a largely alpha-helical bundle of the enzyme BCDHK. This enzyme is the regulator of the dehydrogenase complex that breaks branched-chain amino-acids down, by phosphorylating and thereby inactivating it when synthesis is required. The domain is associated with family HATPase_c pfam02518 which is towards the C-terminal.


:

Pssm-ID: 463093  Cd Length: 158  Bit Score: 193.10  E-value: 3.64e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578804490   56 PLSMKQFLDFGSVNACEKT--SFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAK 133
Cdd:pfam10436   1 PLSLRQLLDFGRSLSEEKLlkSANFLREELPVRLAHRIRELQNLPYILVSNPSISKVYEWYLQSFEELLSFPPPILEDNE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578804490  134 AiyerprrtwlqvsslccmackmiFTDTVIRIRNRHNDVIPTMAQGVIEYKESfgvdpVTSQNVQYFLDRFYMSRISIRM 213
Cdd:pfam10436  81 K-----------------------FTELLEEILDRHNDVVPTLAQGVLELKKY-----LSPEEIQSFLDRFLRSRIGIRL 132

                         170       180
                  ....*....|....*....|....*..
gi 578804490  214 LLNQHSLLFGGKgKGSPSHRKHIGSIN 240
Cdd:pfam10436 133 LAEQHIALTEQS-NNPSHPPDYVGIID 158
HATPase super family cl00075
Histidine kinase-like ATPase domain; This superfamily includes the histidine kinase-like ...
 244-279 5.83e-06

Histidine kinase-like ATPase domain; This superfamily includes the histidine kinase-like ATPase (HATPase) domains of several ATP-binding proteins such as histidine kinase, DNA gyrase B, topoisomerases, heat shock protein 90 (HSP90), phytochrome-like ATPases and DNA mismatch repair proteins. Domains belonging to this superfamily are also referred to as GHKL (gyrase, heat-shock protein 90, histidine kinase, MutL) ATPase domains.


The actual alignment was detected with superfamily member cd16929:

Pssm-ID: 469604 [Multi-domain]  Cd Length: 169  Bit Score: 45.41  E-value: 5.83e-06
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 578804490 244 NVLEVIKDGYENARRLCDLYYINSPELELEELNVIE 279
Cdd:cd16929    1 SPKKVVEDASEEARVLCDDYYLSSPELEIEGDPSIR 36
 
Name Accession Description Interval E-value
BCDHK_Adom3 pfam10436
Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase; Catabolism and synthesis of ...
 56-240 3.64e-62

Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase; Catabolism and synthesis of leucine, isoleucine and valine are finely balanced, allowing the body to make the most of dietary input but removing excesses to prevent toxic build-up of their corresponding keto-acids. This is the butyryl-CoA dehydrogenase, subunit A domain 3, a largely alpha-helical bundle of the enzyme BCDHK. This enzyme is the regulator of the dehydrogenase complex that breaks branched-chain amino-acids down, by phosphorylating and thereby inactivating it when synthesis is required. The domain is associated with family HATPase_c pfam02518 which is towards the C-terminal.


Pssm-ID: 463093  Cd Length: 158  Bit Score: 193.10  E-value: 3.64e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578804490   56 PLSMKQFLDFGSVNACEKT--SFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAK 133
Cdd:pfam10436   1 PLSLRQLLDFGRSLSEEKLlkSANFLREELPVRLAHRIRELQNLPYILVSNPSISKVYEWYLQSFEELLSFPPPILEDNE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578804490  134 AiyerprrtwlqvsslccmackmiFTDTVIRIRNRHNDVIPTMAQGVIEYKESfgvdpVTSQNVQYFLDRFYMSRISIRM 213
Cdd:pfam10436  81 K-----------------------FTELLEEILDRHNDVVPTLAQGVLELKKY-----LSPEEIQSFLDRFLRSRIGIRL 132

                         170       180
                  ....*....|....*....|....*..
gi 578804490  214 LLNQHSLLFGGKgKGSPSHRKHIGSIN 240
Cdd:pfam10436 133 LAEQHIALTEQS-NNPSHPPDYVGIID 158
HATPase_PDK-like cd16929
Histidine kinase-like ATPase domain of pyruvate dehydrogenase kinase, branched-chain ...
 244-279 5.83e-06

Histidine kinase-like ATPase domain of pyruvate dehydrogenase kinase, branched-chain alpha-ketoacid dehydrogenase kinase and related domains; This family includes the histidine kinase-like ATPase (HATPase) domains of all four PDK isoforms (pyruvate dehydrogenase kinases 1-4) that have been described in mammals, and other PDKs including Saccharomyces Pkp1p and Pkp2p. PDKs and phosphatases tightly regulate the mitochondrial pyruvate dehydrogenase complex (PDC) by reversible phosphorylation. PDC catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA, connecting glycolysis and the TCA acid cycle. Also included in this family is mammalian branched-chain alpha-ketoacid dehydrogenase kinase (BDK), a mitochondrial protein kinase that phosphorylates a subunit of the branched-chain a-ketoacid dehydrogenase (BCKD) complex, which catalyzes the oxidative decarboxylation of branched-chain alpha-ketoacids derived from leucine, isoleucine, and valine, a rate-limiting step in the oxidative degradation of these branched-chain amino acids.


Pssm-ID: 340406 [Multi-domain]  Cd Length: 169  Bit Score: 45.41  E-value: 5.83e-06
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 578804490 244 NVLEVIKDGYENARRLCDLYYINSPELELEELNVIE 279
Cdd:cd16929    1 SPKKVVEDASEEARVLCDDYYLSSPELEIEGDPSIR 36
 
Name Accession Description Interval E-value
BCDHK_Adom3 pfam10436
Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase; Catabolism and synthesis of ...
 56-240 3.64e-62

Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase; Catabolism and synthesis of leucine, isoleucine and valine are finely balanced, allowing the body to make the most of dietary input but removing excesses to prevent toxic build-up of their corresponding keto-acids. This is the butyryl-CoA dehydrogenase, subunit A domain 3, a largely alpha-helical bundle of the enzyme BCDHK. This enzyme is the regulator of the dehydrogenase complex that breaks branched-chain amino-acids down, by phosphorylating and thereby inactivating it when synthesis is required. The domain is associated with family HATPase_c pfam02518 which is towards the C-terminal.


Pssm-ID: 463093  Cd Length: 158  Bit Score: 193.10  E-value: 3.64e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578804490   56 PLSMKQFLDFGSVNACEKT--SFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAK 133
Cdd:pfam10436   1 PLSLRQLLDFGRSLSEEKLlkSANFLREELPVRLAHRIRELQNLPYILVSNPSISKVYEWYLQSFEELLSFPPPILEDNE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578804490  134 AiyerprrtwlqvsslccmackmiFTDTVIRIRNRHNDVIPTMAQGVIEYKESfgvdpVTSQNVQYFLDRFYMSRISIRM 213
Cdd:pfam10436  81 K-----------------------FTELLEEILDRHNDVVPTLAQGVLELKKY-----LSPEEIQSFLDRFLRSRIGIRL 132

                         170       180
                  ....*....|....*....|....*..
gi 578804490  214 LLNQHSLLFGGKgKGSPSHRKHIGSIN 240
Cdd:pfam10436 133 LAEQHIALTEQS-NNPSHPPDYVGIID 158
HATPase_PDK-like cd16929
Histidine kinase-like ATPase domain of pyruvate dehydrogenase kinase, branched-chain ...
 244-279 5.83e-06

Histidine kinase-like ATPase domain of pyruvate dehydrogenase kinase, branched-chain alpha-ketoacid dehydrogenase kinase and related domains; This family includes the histidine kinase-like ATPase (HATPase) domains of all four PDK isoforms (pyruvate dehydrogenase kinases 1-4) that have been described in mammals, and other PDKs including Saccharomyces Pkp1p and Pkp2p. PDKs and phosphatases tightly regulate the mitochondrial pyruvate dehydrogenase complex (PDC) by reversible phosphorylation. PDC catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA, connecting glycolysis and the TCA acid cycle. Also included in this family is mammalian branched-chain alpha-ketoacid dehydrogenase kinase (BDK), a mitochondrial protein kinase that phosphorylates a subunit of the branched-chain a-ketoacid dehydrogenase (BCKD) complex, which catalyzes the oxidative decarboxylation of branched-chain alpha-ketoacids derived from leucine, isoleucine, and valine, a rate-limiting step in the oxidative degradation of these branched-chain amino acids.


Pssm-ID: 340406 [Multi-domain]  Cd Length: 169  Bit Score: 45.41  E-value: 5.83e-06
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 578804490 244 NVLEVIKDGYENARRLCDLYYINSPELELEELNVIE 279
Cdd:cd16929    1 SPKKVVEDASEEARVLCDDYYLSSPELEIEGDPSIR 36
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH