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Conserved domains on  [gi|578825266|ref|XP_006720016|]
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probable cysteine--tRNA ligase, mitochondrial isoform X2 [Homo sapiens]

Protein Classification

cysteine--tRNA ligase( domain architecture ID 1002819)

cysteine--tRNA ligase catalyzes the attachment of cysteine to tRNA(Cys)

EC:  6.1.1.16
Gene Ontology:  GO:0005524|GO:0006423|GO:0004817|GO:0046872|GO:0000049

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
PTZ00399 super family cl36555
cysteinyl-tRNA-synthetase; Provisional
 1-450 3.20e-149

cysteinyl-tRNA-synthetase; Provisional


The actual alignment was detected with superfamily member PTZ00399:

Pssm-ID: 240402 [Multi-domain]  Cd Length: 651  Bit Score: 439.46  E-value: 3.20e-149
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   1 MVMGITDVDDKIIKRANEMNISPAS-LASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYStAKGN 79
Cdd:PTZ00399 102 YVMNITDIDDKIIKRAREEKLSIFLeLARKWEKEFFEDMKALNVRPPDVITRVSEYVPEIVDFIQKIIDNGFAYE-SNGS 180

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  80 VYFDLKS-RGDK--YGKLVgvvPGPV--------GEPA----DSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIEC 144
Cdd:PTZ00399 181 VYFDVEAfRKAGhvYPKLE---PESVadedriaeGEGAlgkvSGEKRSPNDFALWKASKPGEPSWDSPWGKGRPGWHIEC 257

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 145 SAIASMVFGSQLDIHSGGIDLAFPHHENEIAQCEVFHQCEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSP 224
Cdd:PTZ00399 258 SAMASNILGDPIDIHSGGIDLKFPHHDNELAQSEAYFDKHQWVNYFLHSGHLHIKG--LKMSKSLKNFITIRQALSKYTA 335

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 225 DVFRFFCLRSSYRSAIDYSDSAMLQAQQLLLGLGSFLEDARAYMKgQLACGSVR-----EAMLWERLSSTKRAVKAALAD 299
Cdd:PTZ00399 336 RQIRLLFLLHKWDKPMNYSDESMDEAIEKDKVFFNFFANVKIKLR-ESELTSPQkwtqhDFELNELFEETKSAVHAALLD 414

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 300 DFDTPRVVDAILGLAhhgnGQLRASLKEPEGPRSPAVFgAIISYFEQFFETVGISLANQQYVSGD--GSEATLHGVVDEL 377
Cdd:PTZ00399 415 NFDTPEALQALQKLI----SATNTYLNSGEQPSAPLLR-SVAQYVTKILSIFGLVEGSDGLGSQGqnSTSENFKPLLEAL 489

                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 578825266 378 VRFRQKVRQFALA-MPEATGDARRQQllerqpLLEACDTLRR-GLTAHGINIKDRSSTTSTWELLD----QRTKDQKSA 450
Cdd:PTZ00399 490 LRFRDEVRDAAKAeMKLISLDKKKKQ------LLQLCDKLRDeWLPNLGIRIEDKPDGPSVWKLDDkeelQREKEEKEA 562
 
Name Accession Description Interval E-value
PTZ00399 PTZ00399
cysteinyl-tRNA-synthetase; Provisional
 1-450 3.20e-149

cysteinyl-tRNA-synthetase; Provisional


Pssm-ID: 240402 [Multi-domain]  Cd Length: 651  Bit Score: 439.46  E-value: 3.20e-149
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   1 MVMGITDVDDKIIKRANEMNISPAS-LASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYStAKGN 79
Cdd:PTZ00399 102 YVMNITDIDDKIIKRAREEKLSIFLeLARKWEKEFFEDMKALNVRPPDVITRVSEYVPEIVDFIQKIIDNGFAYE-SNGS 180

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  80 VYFDLKS-RGDK--YGKLVgvvPGPV--------GEPA----DSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIEC 144
Cdd:PTZ00399 181 VYFDVEAfRKAGhvYPKLE---PESVadedriaeGEGAlgkvSGEKRSPNDFALWKASKPGEPSWDSPWGKGRPGWHIEC 257

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 145 SAIASMVFGSQLDIHSGGIDLAFPHHENEIAQCEVFHQCEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSP 224
Cdd:PTZ00399 258 SAMASNILGDPIDIHSGGIDLKFPHHDNELAQSEAYFDKHQWVNYFLHSGHLHIKG--LKMSKSLKNFITIRQALSKYTA 335

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 225 DVFRFFCLRSSYRSAIDYSDSAMLQAQQLLLGLGSFLEDARAYMKgQLACGSVR-----EAMLWERLSSTKRAVKAALAD 299
Cdd:PTZ00399 336 RQIRLLFLLHKWDKPMNYSDESMDEAIEKDKVFFNFFANVKIKLR-ESELTSPQkwtqhDFELNELFEETKSAVHAALLD 414

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 300 DFDTPRVVDAILGLAhhgnGQLRASLKEPEGPRSPAVFgAIISYFEQFFETVGISLANQQYVSGD--GSEATLHGVVDEL 377
Cdd:PTZ00399 415 NFDTPEALQALQKLI----SATNTYLNSGEQPSAPLLR-SVAQYVTKILSIFGLVEGSDGLGSQGqnSTSENFKPLLEAL 489

                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 578825266 378 VRFRQKVRQFALA-MPEATGDARRQQllerqpLLEACDTLRR-GLTAHGINIKDRSSTTSTWELLD----QRTKDQKSA 450
Cdd:PTZ00399 490 LRFRDEVRDAAKAeMKLISLDKKKKQ------LLQLCDKLRDeWLPNLGIRIEDKPDGPSVWKLDDkeelQREKEEKEA 562
CysS COG0215
Cysteinyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Cysteinyl-tRNA ...
 2-439 5.80e-142

Cysteinyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Cysteinyl-tRNA synthetase is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 439985 [Multi-domain]  Cd Length: 465  Bit Score: 414.11  E-value: 5.80e-142
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   2 VMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYsTAKGNVY 81
Cdd:COG0215   64 VRNITDVDDKIIKRAAEEGESIWELAERYIAAFHEDMDALGVLPPDIEPRATEHIPEMIELIERLIEKGHAY-EADGDVY 142

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  82 FDLKSRGDkYGKLVGVVP-----GPVGEPaDSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQL 156
Cdd:COG0215  143 FDVRSFPD-YGKLSGRNLddlraGARVEV-DEEKRDPLDFALWKAAKPGEPSWDSPWGRGRPGWHIECSAMSTKYLGETF 220

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 157 DIHSGGIDLAFPHHENEIAQCEVFHqCEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSY 236
Cdd:COG0215  221 DIHGGGIDLIFPHHENEIAQSEAAT-GKPFARYWMHNGFLTVNG--EKMSKSLGNFFTVRDLLKKYDPEVLRFFLLSAHY 297

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 237 RSAIDYSDSAmlqaqqlllglgsfLEDAR--------AYMKGQLACGSVREAMlwERLSSTKRAVKAALADDFDTPRVVD 308
Cdd:COG0215  298 RSPLDFSEEA--------------LEEAEkalerlynALRRLEEALGAADSSA--EEIEELREEFIAAMDDDFNTPEALA 361

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 309 AILGLAHHGNgqlraslKEPEGPRSPAVFGAIISYFEQFFETVGISLANQQYVSGDGSEATLHGVVDELVRFRQKVRQ-- 386
Cdd:COG0215  362 VLFELVREIN-------KALDEGEDKAALAALAALLRALGGVLGLLLLEPEAWQGAAEDELLDALIEALIEERAEARKak 434

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....
gi 578825266 387 -FALAmpeatgdarrqqllerqplleacDTLRRGLTAHGINIKDRSSTTsTWEL 439
Cdd:COG0215  435 dFARA-----------------------DRIRDELAALGIVLEDTPDGT-TWRR 464
tRNA-synt_1e pfam01406
tRNA synthetases class I (C) catalytic domain; This family includes only cysteinyl tRNA ...
 2-247 1.77e-112

tRNA synthetases class I (C) catalytic domain; This family includes only cysteinyl tRNA synthetases.


Pssm-ID: 396128 [Multi-domain]  Cd Length: 301  Bit Score: 333.18  E-value: 1.77e-112
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266    2 VMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYSTAKGNVY 81
Cdd:pfam01406  51 VQNFTDIDDKIIKRARQEGESFRQLAARFIEAYTKDMDALNVLPPDLEPRVTEHIDEIIEFIERLIKKGYAYVSDNGDVY 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   82 FDlKSRGDKYGKLVGVVPGPV----GEPADSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQLD 157
Cdd:pfam01406 131 FD-VSSFPDYGKLSGQNLEQLeagaRGEVSEGKRDPLDFALWKASKEGEPSWDSPWGKGRPGWHIECSAMARKYLGDQID 209

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  158 IHSGGIDLAFPHHENEIAQCEVFHQcEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSYR 237
Cdd:pfam01406 210 IHGGGIDLAFPHHENEIAQSEAAFD-KQLANYWLHNGHVMIDG--EKMSKSLGNFFTIRDVLKRYDPEILRYFLLSVHYR 286

                         250
                  ....*....|
gi 578825266  238 SAIDYSDSAM 247
Cdd:pfam01406 287 SPLDFSEELL 296
cysS TIGR00435
cysteinyl-tRNA synthetase; This model finds the cysteinyl-tRNA synthetase from most but not ...
 2-411 8.01e-108

cysteinyl-tRNA synthetase; This model finds the cysteinyl-tRNA synthetase from most but not from all species. The enzyme from one archaeal species, Archaeoglobus fulgidus, is found but the equivalent enzymes from some other Archaea, including Methanococcus jannaschii, are not found, although biochemical evidence suggests that tRNA(Cys) in these species are charged directly with Cys rather than through a misacylation and correction pathway as for tRNA(Gln). [Protein synthesis, tRNA aminoacylation]


Pssm-ID: 273076 [Multi-domain]  Cd Length: 464  Bit Score: 327.03  E-value: 8.01e-108
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266    2 VMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYSTAKGNVY 81
Cdd:TIGR00435  63 VQNITDIDDKIIKRARENGESVYEVSERFIEAYFEDMKALNVLPPDLEPRATEHIDEIIEFIEQLIEKGYAYVSDNGDVY 142

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   82 FDLkSRGDKYGKLVG-----VVPGPVGEPaDSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQL 156
Cdd:TIGR00435 143 FDV-SKFKDYGKLSKqdldqLEAGARVDV-DEAKRNKLDFVLWKSSKEGEPKWDSPWGKGRPGWHIECSAMNDKYLGDQI 220

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  157 DIHSGGIDLAFPHHENEIAQCEVFHQcEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSY 236
Cdd:TIGR00435 221 DIHGGGVDLIFPHHENEIAQSEAAFG-KQLAKYWMHNGFLMIDN--EKMSKSLGNFFTVRDVLKNYDPEILRYFLLSVHY 297

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  237 RSAIDYSDSAMLQAQQLLLGLGSFLEDAR---AYMKGQLACGSVREAMLwerlsstKRAVKAALADDFDTPRVVDAILGL 313
Cdd:TIGR00435 298 RSPLDFSEELLEAAKNALERLYKALRVLDtslAYSGNQSLNKFPDEKEF-------EARFVEAMDDDLNTANALAVLFEL 370

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  314 AHHGNGQlraslkepegPRSPAVFGAIISYFEQFFETVGIslanqqyVSGDGSEATLHGVVDELVRFRQKVRQFALAMPE 393
Cdd:TIGR00435 371 AKSINLT----------FVSKADAALLIEHLIFLESRLGL-------LLGLPSKPVQAGSNDDLGEIEALIEERSIARKE 433

                         410       420
                  ....*....|....*....|.
gi 578825266  394 ---ATGDARRQQLLERQPLLE 411
Cdd:TIGR00435 434 kdfAKADEIRDELAKKGIVLE 454
CysRS_core cd00672
catalytic core domain of cysteinyl tRNA synthetase; Cysteinyl tRNA synthetase (CysRS) ...
 1-243 4.60e-78

catalytic core domain of cysteinyl tRNA synthetase; Cysteinyl tRNA synthetase (CysRS) catalytic core domain. This class I enzyme is a monomer which aminoacylates the 2'-OH of the nucleotide at the 3' of the appropriate tRNA. The core domain is based on the Rossman fold and is responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate. It contains the characteristic class I HIGH and KMSKS motifs, which are involved in ATP binding.


Pssm-ID: 173899 [Multi-domain]  Cd Length: 213  Bit Score: 241.71  E-value: 4.60e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   1 MVMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVtenipqiisfiegiiargnaystakgnv 80
Cdd:cd00672   61 YVQNITDIDDKIIKRAREEGLSWKEVADYYTKEFFEDMKALNVLPPDVVPRV---------------------------- 112

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  81 yfdlksrgdkygklvgvvpgpvgepadsdkrhasdfalwkaakpqevfwaspwgpgrpgWHIECSAIASMVFGSQLDIHS 160
Cdd:cd00672  113 -----------------------------------------------------------WHIECSAMAMKYLGETFDIHG 133

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 161 GGIDLAFPHHENEIAQCEVFHQcEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSYRSAI 240
Cdd:cd00672  134 GGVDLIFPHHENEIAQSEAATG-KPFARYWLHTGHLTIDG--EKMSKSLGNFITVRDALKKYDPEVLRLALLSSHYRSPL 210


                 ...
gi 578825266 241 DYS 243
Cdd:cd00672  211 DFS 213
 
Name Accession Description Interval E-value
PTZ00399 PTZ00399
cysteinyl-tRNA-synthetase; Provisional
 1-450 3.20e-149

cysteinyl-tRNA-synthetase; Provisional


Pssm-ID: 240402 [Multi-domain]  Cd Length: 651  Bit Score: 439.46  E-value: 3.20e-149
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   1 MVMGITDVDDKIIKRANEMNISPAS-LASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYStAKGN 79
Cdd:PTZ00399 102 YVMNITDIDDKIIKRAREEKLSIFLeLARKWEKEFFEDMKALNVRPPDVITRVSEYVPEIVDFIQKIIDNGFAYE-SNGS 180

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  80 VYFDLKS-RGDK--YGKLVgvvPGPV--------GEPA----DSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIEC 144
Cdd:PTZ00399 181 VYFDVEAfRKAGhvYPKLE---PESVadedriaeGEGAlgkvSGEKRSPNDFALWKASKPGEPSWDSPWGKGRPGWHIEC 257

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 145 SAIASMVFGSQLDIHSGGIDLAFPHHENEIAQCEVFHQCEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSP 224
Cdd:PTZ00399 258 SAMASNILGDPIDIHSGGIDLKFPHHDNELAQSEAYFDKHQWVNYFLHSGHLHIKG--LKMSKSLKNFITIRQALSKYTA 335

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 225 DVFRFFCLRSSYRSAIDYSDSAMLQAQQLLLGLGSFLEDARAYMKgQLACGSVR-----EAMLWERLSSTKRAVKAALAD 299
Cdd:PTZ00399 336 RQIRLLFLLHKWDKPMNYSDESMDEAIEKDKVFFNFFANVKIKLR-ESELTSPQkwtqhDFELNELFEETKSAVHAALLD 414

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 300 DFDTPRVVDAILGLAhhgnGQLRASLKEPEGPRSPAVFgAIISYFEQFFETVGISLANQQYVSGD--GSEATLHGVVDEL 377
Cdd:PTZ00399 415 NFDTPEALQALQKLI----SATNTYLNSGEQPSAPLLR-SVAQYVTKILSIFGLVEGSDGLGSQGqnSTSENFKPLLEAL 489

                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 578825266 378 VRFRQKVRQFALA-MPEATGDARRQQllerqpLLEACDTLRR-GLTAHGINIKDRSSTTSTWELLD----QRTKDQKSA 450
Cdd:PTZ00399 490 LRFRDEVRDAAKAeMKLISLDKKKKQ------LLQLCDKLRDeWLPNLGIRIEDKPDGPSVWKLDDkeelQREKEEKEA 562
CysS COG0215
Cysteinyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Cysteinyl-tRNA ...
 2-439 5.80e-142

Cysteinyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Cysteinyl-tRNA synthetase is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 439985 [Multi-domain]  Cd Length: 465  Bit Score: 414.11  E-value: 5.80e-142
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   2 VMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYsTAKGNVY 81
Cdd:COG0215   64 VRNITDVDDKIIKRAAEEGESIWELAERYIAAFHEDMDALGVLPPDIEPRATEHIPEMIELIERLIEKGHAY-EADGDVY 142

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  82 FDLKSRGDkYGKLVGVVP-----GPVGEPaDSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQL 156
Cdd:COG0215  143 FDVRSFPD-YGKLSGRNLddlraGARVEV-DEEKRDPLDFALWKAAKPGEPSWDSPWGRGRPGWHIECSAMSTKYLGETF 220

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 157 DIHSGGIDLAFPHHENEIAQCEVFHqCEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSY 236
Cdd:COG0215  221 DIHGGGIDLIFPHHENEIAQSEAAT-GKPFARYWMHNGFLTVNG--EKMSKSLGNFFTVRDLLKKYDPEVLRFFLLSAHY 297

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 237 RSAIDYSDSAmlqaqqlllglgsfLEDAR--------AYMKGQLACGSVREAMlwERLSSTKRAVKAALADDFDTPRVVD 308
Cdd:COG0215  298 RSPLDFSEEA--------------LEEAEkalerlynALRRLEEALGAADSSA--EEIEELREEFIAAMDDDFNTPEALA 361

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 309 AILGLAHHGNgqlraslKEPEGPRSPAVFGAIISYFEQFFETVGISLANQQYVSGDGSEATLHGVVDELVRFRQKVRQ-- 386
Cdd:COG0215  362 VLFELVREIN-------KALDEGEDKAALAALAALLRALGGVLGLLLLEPEAWQGAAEDELLDALIEALIEERAEARKak 434

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....
gi 578825266 387 -FALAmpeatgdarrqqllerqplleacDTLRRGLTAHGINIKDRSSTTsTWEL 439
Cdd:COG0215  435 dFARA-----------------------DRIRDELAALGIVLEDTPDGT-TWRR 464
tRNA-synt_1e pfam01406
tRNA synthetases class I (C) catalytic domain; This family includes only cysteinyl tRNA ...
 2-247 1.77e-112

tRNA synthetases class I (C) catalytic domain; This family includes only cysteinyl tRNA synthetases.


Pssm-ID: 396128 [Multi-domain]  Cd Length: 301  Bit Score: 333.18  E-value: 1.77e-112
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266    2 VMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYSTAKGNVY 81
Cdd:pfam01406  51 VQNFTDIDDKIIKRARQEGESFRQLAARFIEAYTKDMDALNVLPPDLEPRVTEHIDEIIEFIERLIKKGYAYVSDNGDVY 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   82 FDlKSRGDKYGKLVGVVPGPV----GEPADSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQLD 157
Cdd:pfam01406 131 FD-VSSFPDYGKLSGQNLEQLeagaRGEVSEGKRDPLDFALWKASKEGEPSWDSPWGKGRPGWHIECSAMARKYLGDQID 209

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  158 IHSGGIDLAFPHHENEIAQCEVFHQcEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSYR 237
Cdd:pfam01406 210 IHGGGIDLAFPHHENEIAQSEAAFD-KQLANYWLHNGHVMIDG--EKMSKSLGNFFTIRDVLKRYDPEILRYFLLSVHYR 286

                         250
                  ....*....|
gi 578825266  238 SAIDYSDSAM 247
Cdd:pfam01406 287 SPLDFSEELL 296
cysS TIGR00435
cysteinyl-tRNA synthetase; This model finds the cysteinyl-tRNA synthetase from most but not ...
 2-411 8.01e-108

cysteinyl-tRNA synthetase; This model finds the cysteinyl-tRNA synthetase from most but not from all species. The enzyme from one archaeal species, Archaeoglobus fulgidus, is found but the equivalent enzymes from some other Archaea, including Methanococcus jannaschii, are not found, although biochemical evidence suggests that tRNA(Cys) in these species are charged directly with Cys rather than through a misacylation and correction pathway as for tRNA(Gln). [Protein synthesis, tRNA aminoacylation]


Pssm-ID: 273076 [Multi-domain]  Cd Length: 464  Bit Score: 327.03  E-value: 8.01e-108
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266    2 VMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYSTAKGNVY 81
Cdd:TIGR00435  63 VQNITDIDDKIIKRARENGESVYEVSERFIEAYFEDMKALNVLPPDLEPRATEHIDEIIEFIEQLIEKGYAYVSDNGDVY 142

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   82 FDLkSRGDKYGKLVG-----VVPGPVGEPaDSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQL 156
Cdd:TIGR00435 143 FDV-SKFKDYGKLSKqdldqLEAGARVDV-DEAKRNKLDFVLWKSSKEGEPKWDSPWGKGRPGWHIECSAMNDKYLGDQI 220

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  157 DIHSGGIDLAFPHHENEIAQCEVFHQcEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSY 236
Cdd:TIGR00435 221 DIHGGGVDLIFPHHENEIAQSEAAFG-KQLAKYWMHNGFLMIDN--EKMSKSLGNFFTVRDVLKNYDPEILRYFLLSVHY 297

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  237 RSAIDYSDSAMLQAQQLLLGLGSFLEDAR---AYMKGQLACGSVREAMLwerlsstKRAVKAALADDFDTPRVVDAILGL 313
Cdd:TIGR00435 298 RSPLDFSEELLEAAKNALERLYKALRVLDtslAYSGNQSLNKFPDEKEF-------EARFVEAMDDDLNTANALAVLFEL 370

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  314 AHHGNGQlraslkepegPRSPAVFGAIISYFEQFFETVGIslanqqyVSGDGSEATLHGVVDELVRFRQKVRQFALAMPE 393
Cdd:TIGR00435 371 AKSINLT----------FVSKADAALLIEHLIFLESRLGL-------LLGLPSKPVQAGSNDDLGEIEALIEERSIARKE 433

                         410       420
                  ....*....|....*....|.
gi 578825266  394 ---ATGDARRQQLLERQPLLE 411
Cdd:TIGR00435 434 kdfAKADEIRDELAKKGIVLE 454
CysRS_core cd00672
catalytic core domain of cysteinyl tRNA synthetase; Cysteinyl tRNA synthetase (CysRS) ...
 1-243 4.60e-78

catalytic core domain of cysteinyl tRNA synthetase; Cysteinyl tRNA synthetase (CysRS) catalytic core domain. This class I enzyme is a monomer which aminoacylates the 2'-OH of the nucleotide at the 3' of the appropriate tRNA. The core domain is based on the Rossman fold and is responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate. It contains the characteristic class I HIGH and KMSKS motifs, which are involved in ATP binding.


Pssm-ID: 173899 [Multi-domain]  Cd Length: 213  Bit Score: 241.71  E-value: 4.60e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   1 MVMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVtenipqiisfiegiiargnaystakgnv 80
Cdd:cd00672   61 YVQNITDIDDKIIKRAREEGLSWKEVADYYTKEFFEDMKALNVLPPDVVPRV---------------------------- 112

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  81 yfdlksrgdkygklvgvvpgpvgepadsdkrhasdfalwkaakpqevfwaspwgpgrpgWHIECSAIASMVFGSQLDIHS 160
Cdd:cd00672  113 -----------------------------------------------------------WHIECSAMAMKYLGETFDIHG 133

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 161 GGIDLAFPHHENEIAQCEVFHQcEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSYRSAI 240
Cdd:cd00672  134 GGVDLIFPHHENEIAQSEAATG-KPFARYWLHTGHLTIDG--EKMSKSLGNFITVRDALKKYDPEVLRLALLSSHYRSPL 210


                 ...
gi 578825266 241 DYS 243
Cdd:cd00672  211 DFS 213
PLN02946 PLN02946
cysteine-tRNA ligase
 2-244 6.84e-78

cysteine-tRNA ligase


Pssm-ID: 178532 [Multi-domain]  Cd Length: 557  Bit Score: 252.55  E-value: 6.84e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   2 VMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYSTaKGNVY 81
Cdd:PLN02946 122 VRNFTDVDDKIIARANELGEDPISLSRRYCEEFLSDMAYLHCLPPSVEPRVSDHIPQIIDMIKQILDNGCAYRV-DGDVY 200

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  82 FDLKsRGDKYGKLVG--VVPGPVGE--PADSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQLD 157
Cdd:PLN02946 201 FSVD-KFPEYGKLSGrkLEDNRAGErvAVDSRKKNPADFALWKAAKEGEPFWDSPWGPGRPGWHIECSAMSAAYLGHSFD 279

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 158 IHSGGIDLAFPHHENEIAQ----CevfhqCEQWGNYFLHSGHLHAkgKEEKMSKSLKNYITIKDFLKTFSPDVFRFFCLR 233
Cdd:PLN02946 280 IHGGGMDLVFPHHENEIAQscaaC-----CDSNISYWIHNGFVTV--DSEKMSKSLGNFFTIRQVIDLYHPLALRLFLLG 352

                        250
                 ....*....|.
gi 578825266 234 SSYRSAIDYSD 244
Cdd:PLN02946 353 THYRSPINYSD 363
cysS PRK14535
cysteinyl-tRNA synthetase; Provisional
 2-326 1.27e-68

cysteinyl-tRNA synthetase; Provisional


Pssm-ID: 173001 [Multi-domain]  Cd Length: 699  Bit Score: 231.14  E-value: 1.27e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   2 VMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYSTAKGNVY 81
Cdd:PRK14535 290 VRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADALGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVY 369

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  82 FDLKSRGdKYGKLVGVVPGPV--GEPADSD--KRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQLD 157
Cdd:PRK14535 370 YAVREFA-AYGQLSGKSLDDLraGERVEVDgfKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFGDTFD 448

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 158 IHSGGIDLAFPHHENEIAQ--------CEVFHQCEQWGN-------YFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTF 222
Cdd:PRK14535 449 IHGGGADLQFPHHENEIAQsvgatghtCGHHHAQTHHGQsiashvkYWLHNGFIRVDG--EKMSKSLGNFFTIREVLKQY 526

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 223 SPDVFRFFCLRSSYRSAIDYSDSAMLQAQQLLLGLGSFLEDARAymkgqlacgsvREAMLWERLSSTKRAVKAALADDFD 302
Cdd:PRK14535 527 DPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTRLYTTLKNTPA-----------AEFMLSENVNDYTRRFYAAMNDDFG 595

                        330       340
                 ....*....|....*....|....*...
gi 578825266 303 TPRVVDAILGLAHHGN----GQLRASLK 326
Cdd:PRK14535 596 TVEAVAVLFELAGEVNktndAQLAGCLK 623
cysS PRK14536
cysteinyl-tRNA synthetase; Provisional
 2-438 1.76e-59

cysteinyl-tRNA synthetase; Provisional


Pssm-ID: 184731 [Multi-domain]  Cd Length: 490  Bit Score: 202.46  E-value: 1.76e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   2 VMGITDV----------DDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGN 71
Cdd:PRK14536  65 VMNITDVghltddadsgEDKMVKSAQEHGKSVLEIAAHYTAAFFRDTARLNIERPSIVCNATEHIQDMIALIKRLEARGH 144

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  72 AYsTAKGNVYFDLKSRGDkYGKLVGVVPGPVGEPA----DSDKRHASDFALW---KAAKPQEVFWASPWGPGRPGWHIEC 144
Cdd:PRK14536 145 TY-CAGGNVYFDIRTFPS-YGSLASAAVEDLQAGAriehDTNKRNPHDFVLWftrSKFENHALTWDSPWGRGYPGWHIEC 222

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 145 SAIASMVFGSQLDIHSGGIDLAFPHHENEIAQCEVFhQCEQWGNYFLHSGHL-HAKGkeeKMSKSLKNYITIKDFL-KTF 222
Cdd:PRK14536 223 SAMSMKYLGEQCDIHIGGVDHIRVHHTNEIAQCEAA-TGKPWVRYWLHHEFLlMNKG---KMSKSAGQFLTLSSLQeKGF 298

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 223 SPDVFRFFCLRSSYRSAIDYSDSAMLQAQQLLLGLGSFLedARAYMKGQLACGSVREAM--LWERLSSTKRAVK------ 294
Cdd:PRK14536 299 QPLDYRFFLLGGHYRSQLAFSWEALKTAKAARRSLVRRV--ARVVDAARATTGSVRGTLaeCAAERVAESRASEselllt 376

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 295 ---AALADDFDTPRVVDAILGLahhgngqlrasLKEPEGPRspavfGAIISYFEQFFETVGISL--ANQQYVSGDGSEAT 369
Cdd:PRK14536 377 dfrAALEDDFSTPKALSELQKL-----------VKDTSVPP-----SLCLSVLQAMDTVLGLGLiqEATASLSAQVPAGP 440

                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 578825266 370 LHGVVDELVRFRQKVRQ---FALAmpeatgdarrqqllerqplleacDTLRRGLTAHGINIKDRSSTTsTWE 438
Cdd:PRK14536 441 SEEEIGQLIEARAHARQtkdFPLA-----------------------DEIRDKLKAEGIELEDTHLGT-IWK 488
mycothiol_MshC TIGR03447
cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase; Members of this ...
 2-332 3.08e-54

cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase; Members of this protein family are MshC, l-cysteine:1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase, an enzyme that uses ATP to ligate a Cys residue to a mycothiol precursor molecule, in the second to last step in mycothiol biosynthesis. This enzyme shows considerable homology to Cys--tRNA ligases, and many instances are misannotated as such. Mycothiol is found in Mycobacterium tuberculosis, Corynebacterium glutamicum, Streptomyces coelicolor, and various other members of the Actinobacteria. Mycothiol is an analog to glutathione. [Biosynthesis of cofactors, prosthetic groups, and carriers, Glutathione and analogs]


Pssm-ID: 132488 [Multi-domain]  Cd Length: 411  Bit Score: 186.47  E-value: 3.08e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266    2 VMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAY---STAKG 78
Cdd:TIGR03447  78 VQNVTDVDDPLFERAERDGVDWRELGTSQIDLFREDMEALRVLPPRDYIGAVESIDEVVEMVEKLLASGAAYiveGPEYP 157

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   79 NVYFDLKSrGDKYGKLVGVVPGPV--------GEPADSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASM 150
Cdd:TIGR03447 158 DVYFSIDA-TEQFGYESGYDRATMlelfaergGDPDRPGKRDPLDALLWRAAREGEPSWDSPFGRGRPGWHIECSAIALN 236

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  151 VFGSQLDIHSGGIDLAFPHHENEIAQCEVFHQCEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKT-FSPDVFRF 229
Cdd:TIGR03447 237 RLGAGFDIQGGGSDLIFPHHEFSAAHAEAATGVRRMARHYVHAGMIGLDG--EKMSKSLGNLVFVSKLRAAgVDPAAIRL 314

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  230 FCLRSSYRSAIDYSDSAMLQAqqlllglgsflEDARAYMKGQLACGSVREAmlwerlSSTKRAVKAALADDFDTPRVVDA 309
Cdd:TIGR03447 315 GLLAGHYRQDRDWTDAVLAEA-----------EARLARWRAALALPDAPDA------TDLIARLRQHLANDLDTPAALAA 377

                         330       340
                  ....*....|....*....|...
gi 578825266  310 ILGLAhhgNGQLRASLKEPEGPR 332
Cdd:TIGR03447 378 VDGWA---ADALSYGGSDTEAPA 397
PRK12418 PRK12418
cysteinyl-tRNA synthetase; Provisional
 2-310 8.62e-54

cysteinyl-tRNA synthetase; Provisional


Pssm-ID: 183518 [Multi-domain]  Cd Length: 384  Bit Score: 184.36  E-value: 8.62e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   2 VMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAY---STAKG 78
Cdd:PRK12418  51 VQNVTDVDDPLLERAARDGVDWRDLAEREIALFREDMEALRVLPPRDYVGAVESIPEVVELVEKLLASGAAYvvdDEEYP 130

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  79 NVYFDLkSRGDKYGKLVGVVPGPV--------GEPADSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASM 150
Cdd:PRK12418 131 DVYFSV-DATPQFGYESGYDRATMlelfaergGDPDRPGKRDPLDALLWRAARPGEPSWPSPFGPGRPGWHIECSAIALN 209

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 151 VFGSQLDIHSGGIDLAFPHHENEIAQCEVFHQCEQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKT-FSPDVFRF 229
Cdd:PRK12418 210 RLGSGFDIQGGGSDLIFPHHEFSAAHAEAATGERRFARHYVHAGMIGLDG--EKMSKSRGNLVFVSRLRAAgVDPAAIRL 287

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 230 FCLRSSYRSAIDYSDSAmlqaqqlllglgsfLEDARAYM---KGQLACGSVREAMlwerlsSTKRAVKAALADDFDTPRV 306
Cdd:PRK12418 288 ALLAGHYRADREWTDAV--------------LAEAEARLarwRAAAALPAGPDAA------DVVARVRAALADDLDTPGA 347


                 ....
gi 578825266 307 VDAI 310
Cdd:PRK12418 348 LAAV 351
cysS PRK14534
cysteinyl-tRNA synthetase; Provisional
 3-243 9.77e-45

cysteinyl-tRNA synthetase; Provisional


Pssm-ID: 173000 [Multi-domain]  Cd Length: 481  Bit Score: 162.71  E-value: 9.77e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266   3 MGITDV----------DDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNA 72
Cdd:PRK14534  64 MNITDIghltgdfddgEDKVVKAARERGLTVYEISRFFTEAFFDDCKKLNIVYPDKVLVASEYIPIMIEVVKVLEENGFT 143

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266  73 YsTAKGNVYFDLkSRGDKYGKLVGVVPGPVGEPA------DSDKRHASDFALW---KAAKPQEVFWASPWGPGRPGWHIE 143
Cdd:PRK14534 144 Y-FVNGNVYFDT-SCFKSYGQMAGINLNDFKDMSvsrveiDKSKRNKSDFVLWftnSKFKDQEMKWDSPWGFGYPSWHLE 221

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 144 CSAIASMVFGSQLDIHSGGIDLAFPHHENEIAQCEVFHQcEQWGNYFLHSGHLHAkgKEEKMSKSLKNYITIKDF-LKTF 222
Cdd:PRK14534 222 CAAMNLEYFKSTLDIHLGGVDHIGVHHINEIAIAECYLN-KKWCDMFVHGEFLIM--EYEKMSKSNNNFITIKDLeDQGF 298

                        250       260
                 ....*....|....*....|.
gi 578825266 223 SPDVFRFFCLRSSYRSAIDYS 243
Cdd:PRK14534 299 SPLDFRYFCLTAHYRTQLKFT 319
MetRS_core cd00814
catalytic core domain of methioninyl-tRNA synthetases; Methionine tRNA synthetase (MetRS) ...
 193-235 5.64e-07

catalytic core domain of methioninyl-tRNA synthetases; Methionine tRNA synthetase (MetRS) catalytic core domain. This class I enzyme aminoacylates the 2'-OH of the nucleotide at the 3' of the appropriate tRNA. MetRS, which consists of the core domain and an anti-codon binding domain, functions as a monomer. However, in some species the anti-codon binding domain is followed by an EMAP domain. In this case, MetRS functions as a homodimer. The core domain is based on the Rossman fold and is responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate. It contains the characteristic class I HIGH and KMSKS motifs, which are involved in ATP binding. As a result of a deletion event, MetRS has a significantly shorter core domain insertion than IleRS, ValRS, and LeuR. Consequently, the MetRS insertion lacks the editing function.


Pssm-ID: 173907 [Multi-domain]  Cd Length: 319  Bit Score: 50.99  E-value: 5.64e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 578825266 193 SGHLHAKGKeeKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSS 235
Cdd:cd00814  271 HGYLTVEGK--KMSKSRGNVVDPDDLLERYGADALRYYLLRER 311
LeuRS_core cd00812
catalytic core domain of leucyl-tRNA synthetases; Leucyl tRNA synthetase (LeuRS) catalytic ...
 156-235 9.71e-07

catalytic core domain of leucyl-tRNA synthetases; Leucyl tRNA synthetase (LeuRS) catalytic core domain. This class I enzyme is a monomer which aminoacylates the 2'-OH of the nucleotide at the 3' of the appropriate tRNA. The core domain is based on the Rossman fold and is responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate. It contains the characteristic class I HIGH and KMSKS motifs, which are involved in ATP binding. In Aquifex aeolicus, the gene encoding LeuRS is split in two, just before the KMSKS motif. Consequently, LeuRS is a heterodimer, which likely superimposes with the LeuRS monomer found in most other organisms. LeuRS has an insertion in the core domain, which is subject to both deletions and rearrangements and thus differs between prokaryotic LeuRS and archaeal/eukaryotic LeuRS. This editing region hydrolyzes mischarged cognate tRNAs and thus prevents the incorporation of chemically similar amino acids.


Pssm-ID: 173906 [Multi-domain]  Cd Length: 314  Bit Score: 50.32  E-value: 9.71e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578825266 156 LDIHSGGIDLA--------FPHHeneiaqceVFHQCEQWGNY----FLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFS 223
Cdd:cd00812  225 VDIYIGGKEHApnhllysrFNHK--------ALFDEGLVTDEppkgLIVQGMVLLEG--EKMSKSKGNVVTPDEAIKKYG 294

                         90
                 ....*....|..
gi 578825266 224 PDVFRFFCLRSS 235
Cdd:cd00812  295 ADAARLYILFAA 306
MetG COG0143
Methionyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Methionyl-tRNA ...
 193-233 1.70e-06

Methionyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Methionyl-tRNA synthetase is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 439913 [Multi-domain]  Cd Length: 544  Bit Score: 50.11  E-value: 1.70e-06
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 578825266 193 SGHLHAKGkeEKMSKSLKNYITIKDFLKTFSPDVFRFFCLR 233
Cdd:COG0143  318 HGFLTVEG--EKMSKSRGNVIDPDDLLDRYGPDALRYYLLR 356
tRNA-synt_1g pfam09334
tRNA synthetases class I (M); This family includes methionyl tRNA synthetases.
 193-245 3.72e-05

tRNA synthetases class I (M); This family includes methionyl tRNA synthetases.


Pssm-ID: 401322 [Multi-domain]  Cd Length: 387  Bit Score: 45.74  E-value: 3.72e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 578825266  193 SGHLHAKGKeeKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSYRSA-IDYSDS 245
Cdd:pfam09334 315 HGYLTYEGG--KMSKSRGNVVWPSEALDRFPPDALRYYLARNRPETKdTDFSWE 366
IleS COG0060
Isoleucyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Isoleucyl-tRNA ...
 194-244 1.54e-04

Isoleucyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Isoleucyl-tRNA synthetase is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 439830 [Multi-domain]  Cd Length: 931  Bit Score: 44.30  E-value: 1.54e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 578825266 194 GH-LHAKGKeeKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSYRSAIDYSD 244
Cdd:COG0060  594 GFvLDEDGR--KMSKSLGNVVDPQEVIDKYGADILRLWVASSDYWGDLRFSD 643
PRK11893 PRK11893
methionyl-tRNA synthetase; Reviewed
 187-233 2.47e-04

methionyl-tRNA synthetase; Reviewed


Pssm-ID: 237012 [Multi-domain]  Cd Length: 511  Bit Score: 43.33  E-value: 2.47e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 578825266 187 GNYFLhsghlhaKGKEEKMSKSLKNYITIKDFLKTFSPDVFRFFCLR 233
Cdd:PRK11893 289 AHGFL-------TLDGEKMSKSLGNVIDPFDLVDEYGVDAVRYFLLR 328
tRNA-synt_1 pfam00133
tRNA synthetases class I (I, L, M and V); Other tRNA synthetase sub-families are too ...
 188-243 3.52e-04

tRNA synthetases class I (I, L, M and V); Other tRNA synthetase sub-families are too dissimilar to be included.


Pssm-ID: 459685 [Multi-domain]  Cd Length: 602  Bit Score: 42.78  E-value: 3.52e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 578825266  188 NYFLHSGHLHAKGKeeKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSYRSAIDYS 243
Cdd:pfam00133 549 NVLVHGLVRDEQGR--KMSKSLGNVIDPLDVIDKYGADALRLWLANSDYGRDINLS 602
leuS PRK12300
leucyl-tRNA synthetase; Reviewed
 203-230 4.08e-04

leucyl-tRNA synthetase; Reviewed


Pssm-ID: 237049 [Multi-domain]  Cd Length: 897  Bit Score: 42.93  E-value: 4.08e-04
                         10        20
                 ....*....|....*....|....*...
gi 578825266 203 EKMSKSLKNYITIKDFLKTFSPDVFRFF 230
Cdd:PRK12300 576 KKMSKSKGNVIPLRKAIEEYGADVVRLY 603
PLN02959 PLN02959
aminoacyl-tRNA ligase
 184-231 1.41e-03

aminoacyl-tRNA ligase


Pssm-ID: 215518 [Multi-domain]  Cd Length: 1084  Bit Score: 41.21  E-value: 1.41e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 578825266  184 EQWGNYFLHSGHLHAKGkeEKMSKSLKNYITIKDFLKTFSPDVFRFFC 231
Cdd:PLN02959  700 EHWPRGFRCNGHLMLNS--EKMSKSTGNFLTLRQAIEEFSADATRFAL 745
LysS COG1384
Lysyl-tRNA synthetase, class I [Translation, ribosomal structure and biogenesis]; Lysyl-tRNA ...
 203-243 2.63e-03

Lysyl-tRNA synthetase, class I [Translation, ribosomal structure and biogenesis]; Lysyl-tRNA synthetase, class I is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 440994 [Multi-domain]  Cd Length: 525  Bit Score: 40.18  E-value: 2.63e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 578825266 203 EKMSKSLKNYITIKDFLKTFSPDVFRFFCLRsSYRSAIDYS 243
Cdd:COG1384  286 EKISKSKGNGLTVEEWLEYAEPESLRYFMFR-KPKKAKDLD 325
metG PRK00133
methionyl-tRNA synthetase; Reviewed
 203-230 3.14e-03

methionyl-tRNA synthetase; Reviewed


Pssm-ID: 234655 [Multi-domain]  Cd Length: 673  Bit Score: 40.14  E-value: 3.14e-03
                         10        20
                 ....*....|....*....|....*....
gi 578825266 203 EKMSKSlKNY-ITIKDFLKTFSPDVFRFF 230
Cdd:PRK00133 328 AKMSKS-RGTfIWARTYLDHLDPDYLRYY 355
tRNA-synt_1f pfam01921
tRNA synthetases class I (K); This family includes only lysyl tRNA synthetases from ...
 201-241 3.66e-03

tRNA synthetases class I (K); This family includes only lysyl tRNA synthetases from prokaryotes.


Pssm-ID: 396483  Cd Length: 357  Bit Score: 39.55  E-value: 3.66e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 578825266  201 KEEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSYRSAID 241
Cdd:pfam01921 276 GGGKMSSSKGNVITPEDWLEYAPPESLRFLMFRTKPKKAKD 316
PRK12267 PRK12267
methionyl-tRNA synthetase; Reviewed
 195-234 8.56e-03

methionyl-tRNA synthetase; Reviewed


Pssm-ID: 237028 [Multi-domain]  Cd Length: 648  Bit Score: 38.63  E-value: 8.56e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 578825266 195 HLHAKG----KEEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRS 234
Cdd:PRK12267 286 KVFAHGwwlmKDGKMSKSKGNVVDPEELVDRYGLDALRYYLLRE 329
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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