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Conserved domains on  [gi|578830100|ref|XP_006721660|]
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mannose-P-dolichol utilization defect 1 protein isoform X1 [Homo sapiens]

Protein Classification

cystinosin/MPDU1 family protein( domain architecture ID 707892)

cystinosin/MPDU1 family protein similar to homo sapiens mannose-P-dolichol utilization defect 1, a cystinosin homolog that required for normal utilization of mannose-dolichol phosphate (Dol-P-Man) in the synthesis of N-linked and O-linked oligosaccharides and GPI anchors

Gene Ontology:  GO:0006810|GO:0016020
PubMed:  22363504
TCDB:  2.A.43

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
2A43 super family cl28663
Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and ...
 40-129 4.69e-28

Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and amines]


The actual alignment was detected with superfamily member TIGR00951:

Pssm-ID: 130026 [Multi-domain]  Cd Length: 220  Bit Score: 106.86  E-value: 4.69e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578830100   40 ILLSKGLGLGIVAGSLLVKLPQVFKILGAKSAEGLSLQSVMLELVALTGTMVY--------SITNNFPFSSWG---EALF 108
Cdd:TIGR00951   1 ILLSQILGWGYVAAWSISFYPQIIKNWRRKSAEGLSFDFVMLNLVGFTAYVIFnflqlycwSITNEFPLSSPGvtqNDVF 80

                          90       100
                  ....*....|....*....|.
gi 578830100  109 LMLQTITICFLVMHYRGQTVK 129
Cdd:TIGR00951  81 FTLHAILICFIVLHQCGDYER 101
 
Name Accession Description Interval E-value
2A43 TIGR00951
Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and ...
 40-129 4.69e-28

Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 130026 [Multi-domain]  Cd Length: 220  Bit Score: 106.86  E-value: 4.69e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578830100   40 ILLSKGLGLGIVAGSLLVKLPQVFKILGAKSAEGLSLQSVMLELVALTGTMVY--------SITNNFPFSSWG---EALF 108
Cdd:TIGR00951   1 ILLSQILGWGYVAAWSISFYPQIIKNWRRKSAEGLSFDFVMLNLVGFTAYVIFnflqlycwSITNEFPLSSPGvtqNDVF 80

                          90       100
                  ....*....|....*....|.
gi 578830100  109 LMLQTITICFLVMHYRGQTVK 129
Cdd:TIGR00951  81 FTLHAILICFIVLHQCGDYER 101
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
 42-101 5.94e-12

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


Pssm-ID: 461220 [Multi-domain]  Cd Length: 61  Bit Score: 59.44  E-value: 5.94e-12
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 578830100   42 LSKGLGLGIVAGSLLVKLPQVFKILGAKSAEGLSLQSVMLELVALTGTMVYSITNNFPFS 101
Cdd:pfam04193   1 LSNVLGYISLVLWLVSYLPQIIKNYKRKSTEGLSILFLLLWLLGDIFYLLYLILNGFPPS 60
CTNS smart00679
Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, ...
 56-87 9.51e-05

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins; Function unknown, but likely to be associated with the glycosylation machinery.


Pssm-ID: 128923 [Multi-domain]  Cd Length: 32  Bit Score: 38.59  E-value: 9.51e-05
                           10        20        30
                   ....*....|....*....|....*....|..
gi 578830100    56 LVKLPQVFKILGAKSAEGLSLQSVMLELVALT 87
Cdd:smart00679   1 VSLLPQIIKNYRRKSTEGLSILFVLLWLLGDI 32
SWEET COG4095
Sugar transporter, SemiSWEET family, contains PQ motif [Carbohydrate transport and metabolism]; ...
 48-94 7.81e-03

Sugar transporter, SemiSWEET family, contains PQ motif [Carbohydrate transport and metabolism];


Pssm-ID: 443271  Cd Length: 83  Bit Score: 34.78  E-value: 7.81e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 578830100  48 LGIVAGSLLVK--LPQVFKILGAKSAEGLSLQSVMLELVALTGTMVYSI 94
Cdd:COG4095    6 IGLLAGILTTIsfLPQVIKTWKTKSTKDISLGMYLIFTTGVALWLIYGI 54
 
Name Accession Description Interval E-value
2A43 TIGR00951
Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and ...
 40-129 4.69e-28

Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 130026 [Multi-domain]  Cd Length: 220  Bit Score: 106.86  E-value: 4.69e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578830100   40 ILLSKGLGLGIVAGSLLVKLPQVFKILGAKSAEGLSLQSVMLELVALTGTMVY--------SITNNFPFSSWG---EALF 108
Cdd:TIGR00951   1 ILLSQILGWGYVAAWSISFYPQIIKNWRRKSAEGLSFDFVMLNLVGFTAYVIFnflqlycwSITNEFPLSSPGvtqNDVF 80

                          90       100
                  ....*....|....*....|.
gi 578830100  109 LMLQTITICFLVMHYRGQTVK 129
Cdd:TIGR00951  81 FTLHAILICFIVLHQCGDYER 101
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
 42-101 5.94e-12

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


Pssm-ID: 461220 [Multi-domain]  Cd Length: 61  Bit Score: 59.44  E-value: 5.94e-12
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 578830100   42 LSKGLGLGIVAGSLLVKLPQVFKILGAKSAEGLSLQSVMLELVALTGTMVYSITNNFPFS 101
Cdd:pfam04193   1 LSNVLGYISLVLWLVSYLPQIIKNYKRKSTEGLSILFLLLWLLGDIFYLLYLILNGFPPS 60
CTNS smart00679
Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, ...
 56-87 9.51e-05

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins; Function unknown, but likely to be associated with the glycosylation machinery.


Pssm-ID: 128923 [Multi-domain]  Cd Length: 32  Bit Score: 38.59  E-value: 9.51e-05
                           10        20        30
                   ....*....|....*....|....*....|..
gi 578830100    56 LVKLPQVFKILGAKSAEGLSLQSVMLELVALT 87
Cdd:smart00679   1 VSLLPQIIKNYRRKSTEGLSILFVLLWLLGDI 32
SWEET COG4095
Sugar transporter, SemiSWEET family, contains PQ motif [Carbohydrate transport and metabolism]; ...
 48-94 7.81e-03

Sugar transporter, SemiSWEET family, contains PQ motif [Carbohydrate transport and metabolism];


Pssm-ID: 443271  Cd Length: 83  Bit Score: 34.78  E-value: 7.81e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 578830100  48 LGIVAGSLLVK--LPQVFKILGAKSAEGLSLQSVMLELVALTGTMVYSI 94
Cdd:COG4095    6 IGLLAGILTTIsfLPQVIKTWKTKSTKDISLGMYLIFTTGVALWLIYGI 54
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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