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Conserved domains on  [gi|1002263446|ref|XP_015636071|]
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uncharacterized protein [Oryza sativa Japonica Group]

Protein Classification

BRO1_Alix_like domain-containing protein( domain architecture ID 10173280)

BRO1_Alix_like domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
BRO1_Alix_like cd09034
Protein-interacting Bro1-like domain of mammalian Alix and related domains; This superfamily ...
 13-399 8.27e-88

Protein-interacting Bro1-like domain of mammalian Alix and related domains; This superfamily includes the Bro1-like domains of mammalian Alix (apoptosis-linked gene-2 interacting protein X), His-Domain type N23 protein tyrosine phosphatase (HD-PTP, also known as PTPN23), RhoA-binding proteins Rhophilin-1 and Rhophilin-2, Brox, Bro1 and Rim20 (also known as PalA) from Saccharomyces cerevisiae, and related domains. Alix, HD-PTP, Brox, Bro1 and Rim20 interact with the ESCRT (Endosomal Sorting Complexes Required for Transport) system. Alix, also known as apoptosis-linked gene-2 interacting protein 1 (AIP1), participates in membrane remodeling processes during the budding of enveloped viruses, vesicle budding inside late endosomal multivesicular bodies (MVBs), and the abscission reactions of mammalian cell division. It also functions in apoptosis. HD-PTP functions in cell migration and endosomal trafficking, Bro1 in endosomal trafficking, and Rim20 in the response to the external pH via the Rim101 pathway. Bro1-like domains are boomerang-shaped, and part of the domain is a tetratricopeptide repeat (TPR)-like structure. Bro1-like domains bind components of the ESCRT-III complex: CHMP4 (in the case of Alix, HD-PTP, and Brox) and Snf7 (in the case of yeast Bro1, and Rim20). The single domain protein human Brox, and the isolated Bro1-like domains of Alix, HD-PTP and Rhophilin can bind human immunodeficiency virus type 1 (HIV-1) nucleocapsid. Alix, HD-PTP, Bro1, and Rim20 also have a V-shaped (V) domain, which in the case of Alix, has been shown to be a dimerization domain and to contain a binding site for the retroviral late assembly (L) domain YPXnL motif, which is partially conserved in this superfamily. Alix, HD-PTP and Bro1 also have a proline-rich region (PRR); the Alix PRR binds multiple partners. Rhophilin-1, and -2, in addition to this Bro1-like domain, have an N-terminal Rho-binding domain and a C-terminal PDZ (PS.D.-95, Disc-large, ZO-1) domain. HD-PTP is encoded by the PTPN23 gene, a tumor suppressor gene candidate frequently absent in human kidney, breast, lung, and cervical tumors. This protein has a C-terminal, catalytically inactive tyrosine phosphatase domain.


:

Pssm-ID: 185761 [Multi-domain]  Cd Length: 345  Bit Score: 269.99  E-value: 8.27e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  13 GGPRRRPGSVgDVVVFLPGlRVPRSVDFSQALAgrLDEASSRLSSLRARVVdmamqesaaalkpkrrAAARHGSSTANLL 92
Cdd:cd09034     3 GLPLKKTKEV-DVKVPLSK-FIPKNYGELEATA--VEDLIEKLSKLRNNIV----------------TEQNNDTTCENLL 62

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  93 QALEDYLPVLLGLVK--EGSELRHGVHFVWTNQEDNAEETAMaDAWYEVLSVLHLMAMVCLLQANSLLLPRSygdgyapr 170
Cdd:cd09034    63 EALKEYLPYLLGLEKklPFQKLRDNVEFTWTDSFDTKKESAT-SLRYELLSILFNLAALASQLANEKLITGS-------- 133

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 171 vsEESRRATVDVFLKASGYLDCAIRQVLPQISSELrrqlPVDLAEGNLKALSLQALGQGVDMQLGLAIDSPKATLAVKRR 250
Cdd:cd09034   134 --EEDLKQAIKSLQKAAGYFEYLKEHVLPLPPDEL----PVDLTEAVLSALSLIMLAQAQECFLLKAEEDKKAKLSLLAR 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 251 LACEMVKYWHQIQESIPEIP--VSDGWGKKHLLFVKWKYVEAKAAAYYFHGLILDEGNtekSHGMAVAALQASEEFLKES 328
Cdd:cd09034   208 LACEAAKYYEEALKCLSGVDleTIKNIPKKWLLFLKWKKCIFKALAYYYHGLKLDEAN---KIGEAIARLQAALELLKES 284

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1002263446 329 KRASEAFhatpptsrSPTPFGTAKYMLDKIPKDASSKVKINQDLYTQErvIGTPPPLPDFALALKPDDYDL 399
Cdd:cd09034   285 ERLCKSF--------LLDVWGNLKKLKEKIEKELEKAERENDFIYFEE--VPPEDPLPEIKGALLVKPPPL 345
 
Name Accession Description Interval E-value
BRO1_Alix_like cd09034
Protein-interacting Bro1-like domain of mammalian Alix and related domains; This superfamily ...
 13-399 8.27e-88

Protein-interacting Bro1-like domain of mammalian Alix and related domains; This superfamily includes the Bro1-like domains of mammalian Alix (apoptosis-linked gene-2 interacting protein X), His-Domain type N23 protein tyrosine phosphatase (HD-PTP, also known as PTPN23), RhoA-binding proteins Rhophilin-1 and Rhophilin-2, Brox, Bro1 and Rim20 (also known as PalA) from Saccharomyces cerevisiae, and related domains. Alix, HD-PTP, Brox, Bro1 and Rim20 interact with the ESCRT (Endosomal Sorting Complexes Required for Transport) system. Alix, also known as apoptosis-linked gene-2 interacting protein 1 (AIP1), participates in membrane remodeling processes during the budding of enveloped viruses, vesicle budding inside late endosomal multivesicular bodies (MVBs), and the abscission reactions of mammalian cell division. It also functions in apoptosis. HD-PTP functions in cell migration and endosomal trafficking, Bro1 in endosomal trafficking, and Rim20 in the response to the external pH via the Rim101 pathway. Bro1-like domains are boomerang-shaped, and part of the domain is a tetratricopeptide repeat (TPR)-like structure. Bro1-like domains bind components of the ESCRT-III complex: CHMP4 (in the case of Alix, HD-PTP, and Brox) and Snf7 (in the case of yeast Bro1, and Rim20). The single domain protein human Brox, and the isolated Bro1-like domains of Alix, HD-PTP and Rhophilin can bind human immunodeficiency virus type 1 (HIV-1) nucleocapsid. Alix, HD-PTP, Bro1, and Rim20 also have a V-shaped (V) domain, which in the case of Alix, has been shown to be a dimerization domain and to contain a binding site for the retroviral late assembly (L) domain YPXnL motif, which is partially conserved in this superfamily. Alix, HD-PTP and Bro1 also have a proline-rich region (PRR); the Alix PRR binds multiple partners. Rhophilin-1, and -2, in addition to this Bro1-like domain, have an N-terminal Rho-binding domain and a C-terminal PDZ (PS.D.-95, Disc-large, ZO-1) domain. HD-PTP is encoded by the PTPN23 gene, a tumor suppressor gene candidate frequently absent in human kidney, breast, lung, and cervical tumors. This protein has a C-terminal, catalytically inactive tyrosine phosphatase domain.


Pssm-ID: 185761 [Multi-domain]  Cd Length: 345  Bit Score: 269.99  E-value: 8.27e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  13 GGPRRRPGSVgDVVVFLPGlRVPRSVDFSQALAgrLDEASSRLSSLRARVVdmamqesaaalkpkrrAAARHGSSTANLL 92
Cdd:cd09034     3 GLPLKKTKEV-DVKVPLSK-FIPKNYGELEATA--VEDLIEKLSKLRNNIV----------------TEQNNDTTCENLL 62

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  93 QALEDYLPVLLGLVK--EGSELRHGVHFVWTNQEDNAEETAMaDAWYEVLSVLHLMAMVCLLQANSLLLPRSygdgyapr 170
Cdd:cd09034    63 EALKEYLPYLLGLEKklPFQKLRDNVEFTWTDSFDTKKESAT-SLRYELLSILFNLAALASQLANEKLITGS-------- 133

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 171 vsEESRRATVDVFLKASGYLDCAIRQVLPQISSELrrqlPVDLAEGNLKALSLQALGQGVDMQLGLAIDSPKATLAVKRR 250
Cdd:cd09034   134 --EEDLKQAIKSLQKAAGYFEYLKEHVLPLPPDEL----PVDLTEAVLSALSLIMLAQAQECFLLKAEEDKKAKLSLLAR 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 251 LACEMVKYWHQIQESIPEIP--VSDGWGKKHLLFVKWKYVEAKAAAYYFHGLILDEGNtekSHGMAVAALQASEEFLKES 328
Cdd:cd09034   208 LACEAAKYYEEALKCLSGVDleTIKNIPKKWLLFLKWKKCIFKALAYYYHGLKLDEAN---KIGEAIARLQAALELLKES 284

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1002263446 329 KRASEAFhatpptsrSPTPFGTAKYMLDKIPKDASSKVKINQDLYTQErvIGTPPPLPDFALALKPDDYDL 399
Cdd:cd09034   285 ERLCKSF--------LLDVWGNLKKLKEKIEKELEKAERENDFIYFEE--VPPEDPLPEIKGALLVKPPPL 345
BRO1 smart01041
BRO1-like domain; This domain is found in a number proteins including Rhophilin and BRO1. It ...
 28-404 9.93e-41

BRO1-like domain; This domain is found in a number proteins including Rhophilin and BRO1. It is known to have a role in endosomal targeting. ESCRT-III subunit Snf7 binds to a conserved hydrophobic patch in the BRO1 domain that is required for protein complex formation and for the protein-sorting function of BRO1.


Pssm-ID: 214990  Cd Length: 381  Bit Score: 148.65  E-value: 9.93e-41
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446   28 FLP-GLRVPRSVDFSQALAG----RLDEASSRLSSLRARVVDMamqesaaalkpkrRAAARHGSSTANLLQALEDYLPVL 102
Cdd:smart01041   1 LIPlPLKETKEVDFSKPLKDyikeTYSEDSSSYEDEIAELNRL-------------RQAARTPSRDESGLELLLKYYGQL 67

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  103 LGL-VKEGSELRH-GVHFVWTNQEDNAEETAMADAWYEVLSVLHLMAMVCLLQANSLllprsygdgyaPRVSEESRRATV 180
Cdd:smart01041  68 EALeLRFPPPEGQlKLSFTWYDSLDTGVPSTQSSLAFEKASVLFNLGALYSQIAAEQ-----------NRDTEEGLKEAC 136

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  181 DVFLKASGYLDCAIRQVLPqissELRRQLPVDLAEGNLKALSLQALGQGVDMQLGLAI-DSPKATLAVKRRLACEMVKYW 259
Cdd:smart01041 137 KAFQQAAGVFNYLKENFLH----ALSTEPSVDLSPETLSALSSLMLAQAQECFFEKAIlDGMKNKDSLIAKLAAQAAEYY 212

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  260 HQIQESIPEI-PVSDGWGKKHLLFVKWKYVEAKAAAYYFHGLILDEGNtekSHGMAVAALQASEEFLKESKRASEAFhat 338
Cdd:smart01041 213 EEALKALQTSePVKGYIPKSWIKLVQVKAHHFKALAHYYQALDLEEAN---KYGEAIARLQEALERLKEAKKHLRCK--- 286

                          330       340       350       360       370       380
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1002263446  339 pPTSRSPTPFGTAKYMLDKIPKDASSKVKINQDLYTQERVIGTPPPLPDFALALKPDDYDLPPLDP 404
Cdd:smart01041 287 -KLGKADKLQEDLSGLKDVVEEKLKEAEKDNDFIYHERVPDIVSLPPIKKAPLVKPPPFSEVLKGP 351
BRO1 pfam03097
BRO1-like domain; This domain is found in a number proteins including Rhophilin and BRO1. It ...
 78-332 2.95e-05

BRO1-like domain; This domain is found in a number proteins including Rhophilin and BRO1. It is known to have a role in endosomal targeting. ESCRT-III subunit Snf7 binds to a conserved hydrophobic patch in the BRO1 domain that is required for protein complex formation and for the protein-sorting function of BRO1.


Pssm-ID: 460803  Cd Length: 366  Bit Score: 45.65  E-value: 2.95e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  78 RRAAARHGSSTANLLQALEDY---LpVLLGLvKEGSELRHGVHFVWTNQEDNA-EETAMADAWYEVLSVLHLMAMVCLLQ 153
Cdd:pfam03097  45 RQDAVRGANEDESGLDLLYKYyaqL-ELLEL-RFPIDIQIGIEFTWYDAFGTSsKKVSQSSLAFEKASVLFNIAALYSQL 122

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 154 ANSlllprsygdgyAPRVSEESRRATVDVFLKASGYLDcairqvlpQISSELRRQLPVDLAEGNLKALSLQALGQGVDMQ 233
Cdd:pfam03097 123 AAS-----------QNRSTDEGLKRACKYFQQAAGCFQ--------YLKENFLHAPSPDLSPETLKALSNLMLAQAQECF 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 234 LGLAI-DSPKATLAVKrrLACEMVKYWHQIQESIPEIPVSD-GWgkKHLLFVKWKYVeaKAAAYYFHGLILDEgntEKSH 311
Cdd:pfam03097 184 WEKAInDNKKDSLIAK--LAAQVSELYEEALEALKLSGLIDkEW--ISHVQAKAHHF--KALAQYRQALDDEE---AKKY 254

                         250       260
                  ....*....|....*....|.
gi 1002263446 312 GMAVAALQASEEFLKESKRAS 332
Cdd:pfam03097 255 GEEIARLQLALSLLKEALKSD 275
 
Name Accession Description Interval E-value
BRO1_Alix_like cd09034
Protein-interacting Bro1-like domain of mammalian Alix and related domains; This superfamily ...
 13-399 8.27e-88

Protein-interacting Bro1-like domain of mammalian Alix and related domains; This superfamily includes the Bro1-like domains of mammalian Alix (apoptosis-linked gene-2 interacting protein X), His-Domain type N23 protein tyrosine phosphatase (HD-PTP, also known as PTPN23), RhoA-binding proteins Rhophilin-1 and Rhophilin-2, Brox, Bro1 and Rim20 (also known as PalA) from Saccharomyces cerevisiae, and related domains. Alix, HD-PTP, Brox, Bro1 and Rim20 interact with the ESCRT (Endosomal Sorting Complexes Required for Transport) system. Alix, also known as apoptosis-linked gene-2 interacting protein 1 (AIP1), participates in membrane remodeling processes during the budding of enveloped viruses, vesicle budding inside late endosomal multivesicular bodies (MVBs), and the abscission reactions of mammalian cell division. It also functions in apoptosis. HD-PTP functions in cell migration and endosomal trafficking, Bro1 in endosomal trafficking, and Rim20 in the response to the external pH via the Rim101 pathway. Bro1-like domains are boomerang-shaped, and part of the domain is a tetratricopeptide repeat (TPR)-like structure. Bro1-like domains bind components of the ESCRT-III complex: CHMP4 (in the case of Alix, HD-PTP, and Brox) and Snf7 (in the case of yeast Bro1, and Rim20). The single domain protein human Brox, and the isolated Bro1-like domains of Alix, HD-PTP and Rhophilin can bind human immunodeficiency virus type 1 (HIV-1) nucleocapsid. Alix, HD-PTP, Bro1, and Rim20 also have a V-shaped (V) domain, which in the case of Alix, has been shown to be a dimerization domain and to contain a binding site for the retroviral late assembly (L) domain YPXnL motif, which is partially conserved in this superfamily. Alix, HD-PTP and Bro1 also have a proline-rich region (PRR); the Alix PRR binds multiple partners. Rhophilin-1, and -2, in addition to this Bro1-like domain, have an N-terminal Rho-binding domain and a C-terminal PDZ (PS.D.-95, Disc-large, ZO-1) domain. HD-PTP is encoded by the PTPN23 gene, a tumor suppressor gene candidate frequently absent in human kidney, breast, lung, and cervical tumors. This protein has a C-terminal, catalytically inactive tyrosine phosphatase domain.


Pssm-ID: 185761 [Multi-domain]  Cd Length: 345  Bit Score: 269.99  E-value: 8.27e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  13 GGPRRRPGSVgDVVVFLPGlRVPRSVDFSQALAgrLDEASSRLSSLRARVVdmamqesaaalkpkrrAAARHGSSTANLL 92
Cdd:cd09034     3 GLPLKKTKEV-DVKVPLSK-FIPKNYGELEATA--VEDLIEKLSKLRNNIV----------------TEQNNDTTCENLL 62

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  93 QALEDYLPVLLGLVK--EGSELRHGVHFVWTNQEDNAEETAMaDAWYEVLSVLHLMAMVCLLQANSLLLPRSygdgyapr 170
Cdd:cd09034    63 EALKEYLPYLLGLEKklPFQKLRDNVEFTWTDSFDTKKESAT-SLRYELLSILFNLAALASQLANEKLITGS-------- 133

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 171 vsEESRRATVDVFLKASGYLDCAIRQVLPQISSELrrqlPVDLAEGNLKALSLQALGQGVDMQLGLAIDSPKATLAVKRR 250
Cdd:cd09034   134 --EEDLKQAIKSLQKAAGYFEYLKEHVLPLPPDEL----PVDLTEAVLSALSLIMLAQAQECFLLKAEEDKKAKLSLLAR 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 251 LACEMVKYWHQIQESIPEIP--VSDGWGKKHLLFVKWKYVEAKAAAYYFHGLILDEGNtekSHGMAVAALQASEEFLKES 328
Cdd:cd09034   208 LACEAAKYYEEALKCLSGVDleTIKNIPKKWLLFLKWKKCIFKALAYYYHGLKLDEAN---KIGEAIARLQAALELLKES 284

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1002263446 329 KRASEAFhatpptsrSPTPFGTAKYMLDKIPKDASSKVKINQDLYTQErvIGTPPPLPDFALALKPDDYDL 399
Cdd:cd09034   285 ERLCKSF--------LLDVWGNLKKLKEKIEKELEKAERENDFIYFEE--VPPEDPLPEIKGALLVKPPPL 345
BRO1 smart01041
BRO1-like domain; This domain is found in a number proteins including Rhophilin and BRO1. It ...
 28-404 9.93e-41

BRO1-like domain; This domain is found in a number proteins including Rhophilin and BRO1. It is known to have a role in endosomal targeting. ESCRT-III subunit Snf7 binds to a conserved hydrophobic patch in the BRO1 domain that is required for protein complex formation and for the protein-sorting function of BRO1.


Pssm-ID: 214990  Cd Length: 381  Bit Score: 148.65  E-value: 9.93e-41
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446   28 FLP-GLRVPRSVDFSQALAG----RLDEASSRLSSLRARVVDMamqesaaalkpkrRAAARHGSSTANLLQALEDYLPVL 102
Cdd:smart01041   1 LIPlPLKETKEVDFSKPLKDyikeTYSEDSSSYEDEIAELNRL-------------RQAARTPSRDESGLELLLKYYGQL 67

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  103 LGL-VKEGSELRH-GVHFVWTNQEDNAEETAMADAWYEVLSVLHLMAMVCLLQANSLllprsygdgyaPRVSEESRRATV 180
Cdd:smart01041  68 EALeLRFPPPEGQlKLSFTWYDSLDTGVPSTQSSLAFEKASVLFNLGALYSQIAAEQ-----------NRDTEEGLKEAC 136

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  181 DVFLKASGYLDCAIRQVLPqissELRRQLPVDLAEGNLKALSLQALGQGVDMQLGLAI-DSPKATLAVKRRLACEMVKYW 259
Cdd:smart01041 137 KAFQQAAGVFNYLKENFLH----ALSTEPSVDLSPETLSALSSLMLAQAQECFFEKAIlDGMKNKDSLIAKLAAQAAEYY 212

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  260 HQIQESIPEI-PVSDGWGKKHLLFVKWKYVEAKAAAYYFHGLILDEGNtekSHGMAVAALQASEEFLKESKRASEAFhat 338
Cdd:smart01041 213 EEALKALQTSePVKGYIPKSWIKLVQVKAHHFKALAHYYQALDLEEAN---KYGEAIARLQEALERLKEAKKHLRCK--- 286

                          330       340       350       360       370       380
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1002263446  339 pPTSRSPTPFGTAKYMLDKIPKDASSKVKINQDLYTQERVIGTPPPLPDFALALKPDDYDLPPLDP 404
Cdd:smart01041 287 -KLGKADKLQEDLSGLKDVVEEKLKEAEKDNDFIYHERVPDIVSLPPIKKAPLVKPPPFSEVLKGP 351
BRO1_ScBro1_like cd09242
Protein-interacting, N-terminal, Bro1-like domain of Saccharomyces cerevisiae Bro1 and related ...
 116-405 6.58e-08

Protein-interacting, N-terminal, Bro1-like domain of Saccharomyces cerevisiae Bro1 and related proteins; This family contains the N-terminal, Bro1-like domain of Saccharomyces cerevisiae Bro1 and related proteins. It belongs to the BRO1_Alix_like superfamily which also includes the Bro1-like domains of mammalian Alix (apoptosis-linked gene-2 interacting protein X), His-Domain type N23 protein tyrosine phosphatase (HD-PTP, also known as PTPN23), RhoA-binding proteins Rhophilin-1 and -2, Brox, Saccharomyces cerevisiae Rim20 (also known as PalA), Ustilago maydis Rim23 (also known as PalC), and related domains. Alix, HD-PTP, Brox, Bro1, Rim20, and Rim23, interact with the ESCRT (Endosomal Sorting Complexes Required for Transport) system. Bro1 participates in endosomal trafficking. Bro1-like domains are boomerang-shaped, and part of the domain is a tetratricopeptide repeat (TPR)-like structure. Bro1-like domains bind components of the ESCRT-III complex: Snf7 in the case of Bro1. Snf7 binds to a conserved hydrophobic patch on the middle of the concave side of the Bro1 domain. RIM20, and some other members of the BRO1_Alix_like superfamily including Alix, also have a V-shaped (V) domain. In the case of Alix, the V-domain contains a binding site for the retroviral late assembly (L) domain YPXnL motif, which is partially conserved in the superfamily. The Alix V-domain is also a dimerization domain. The C-terminal portion (V-domain and proline rich-region) of Bro1 interacts with Doa4, a protease that deubiquitinates integral membrane proteins sorted into the lumenal vesicles of late-endosomal multivesicular bodies. It interacts with a YPxL motif in the Doa4 catalytic domain to stimulate its deubiquitination activity.


Pssm-ID: 185765  Cd Length: 348  Bit Score: 53.82  E-value: 6.58e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 116 VHFVWTNQEDNAE---ETAMAdawYEVLSVLHLMAMVCllqanslllprsygdgyaPRVSEESRRATVDVFLKASGYLDC 192
Cdd:cd09242    83 VDFTWYDAFYKSKkvkQHSLA---FEKASVLFNIGALL------------------SQLAAEKYREDEDDLKEAITNLQQ 141

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 193 AIrQVLPQISSELRRQLPVDLAEGNLKALSLQALGQGVDMQLGLAIDSP----KATLAVKrrLACEMVKYWHQIQESIPE 268
Cdd:cd09242   142 AA-GCFQYINENFLHAPSVDLQQENVKFLVKLMLAQAQEIFLLKLINGDdaqkKASLISK--LASATANLYESCVEFLKE 218

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 269 IPVS-DGWGKKhllfvKWKY-VEAKAAAY-----YFHGLILDEGNtekSHGMAVAALQASEEFLKESKRASEAFHATPPt 341
Cdd:cd09242   219 IQEKgISYGDP-----KWISlVQCKAHYYkslaaYYHALALEAAG---KYGEAIAYLTQAESILKEANPQKLSLKASAG- 289

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1002263446 342 SRSPTPFGTAKYMLDKIPKDASSKVKINqDLYTQERVigtppplpdfalalkPDDYDLPPLDPL 405
Cdd:cd09242   290 DAAYALNDDFKGQKDTVEEKLKELEKDN-DFIYHDIV---------------PSEVTLPSIKPL 337
BRO1_UmRIM23-like cd09245
Protein-interacting, Bro1-like domain of Ustilago maydis Rim23 (PalC), and related domains; ...
 56-248 2.63e-06

Protein-interacting, Bro1-like domain of Ustilago maydis Rim23 (PalC), and related domains; This family contains the Bro1-like domain of Ustilago maydis Rim23 (also known as PalC), and related proteins. It belongs to the BRO1_Alix_like superfamily which includes the Bro1-like domains of mammalian Alix (apoptosis-linked gene-2 interacting protein X), His-Domain type N23 protein tyrosine phosphatase (HD-PTP, also known as PTPN23), RhoA-binding proteins Rhophilin-1 and -2, Brox, Bro1 and Rim20 (also known as PalA) from Saccharomyces cerevisiae, and related domains. Alix, HD-PTP, Brox, Bro1, Rim20, and Rim23 interact with the ESCRT (Endosomal Sorting Complexes Required for Transport) system. Rim20 and Rim23 participate in the response to the external pH via the Rim101 pathway. Through its Bro1-like domain, Rim23 allows the interaction between the endosomal and plasma membrane complexes. Bro1-like domains are boomerang-shape, and part of the domain is a tetratricopeptide repeat (TPR)-like structure. Intermediates in the Rim101 pathway may play roles in the pathogenesis of fungal corneal infection during Candida albicans keratitis. This family lacks the V-shaped (V) domain found in many members of the BRO1_Alix_like superfamily.


Pssm-ID: 185768  Cd Length: 413  Bit Score: 49.33  E-value: 2.63e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  56 SSLRARVvdmamqesAAALKP-KRRAAARHGSSTANLLQALEDYLPVLLGLVKEGS----ELRHGVHFVW-----TNQED 125
Cdd:cd09245    31 TTARAVL--------RAALKAhKRTPPGSQASNLLTVVKALEEYLPYLLAIDACLShdelILKSEPTFEWrttlsSTSGR 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 126 NAEETAMADAWYEVLSVLHLMAMVCLLQANSLLLPrsygDGYAPR---VSEESRRAT-------VDVFLKASGYLDCAIR 195
Cdd:cd09245   103 ESPRLPLPGLHYELAFVLLTYAYALSNLARSILAP----LGAYETdrsISDASRKQRderlkaaTKLLCKAAGIFDYLAT 178

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1002263446 196 QVLPQISSELRRQ-LPVDLAEGNLKALSLQALGqgvDMQLgLAI---DSPKATLAVK 248
Cdd:cd09245   179 RVLPQWESNRGGApPPPDLSPEVLSALSSLALA---EATL-LAVrklDPYPAAVDKD 231
BRO1 pfam03097
BRO1-like domain; This domain is found in a number proteins including Rhophilin and BRO1. It ...
 78-332 2.95e-05

BRO1-like domain; This domain is found in a number proteins including Rhophilin and BRO1. It is known to have a role in endosomal targeting. ESCRT-III subunit Snf7 binds to a conserved hydrophobic patch in the BRO1 domain that is required for protein complex formation and for the protein-sorting function of BRO1.


Pssm-ID: 460803  Cd Length: 366  Bit Score: 45.65  E-value: 2.95e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  78 RRAAARHGSSTANLLQALEDY---LpVLLGLvKEGSELRHGVHFVWTNQEDNA-EETAMADAWYEVLSVLHLMAMVCLLQ 153
Cdd:pfam03097  45 RQDAVRGANEDESGLDLLYKYyaqL-ELLEL-RFPIDIQIGIEFTWYDAFGTSsKKVSQSSLAFEKASVLFNIAALYSQL 122

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 154 ANSlllprsygdgyAPRVSEESRRATVDVFLKASGYLDcairqvlpQISSELRRQLPVDLAEGNLKALSLQALGQGVDMQ 233
Cdd:pfam03097 123 AAS-----------QNRSTDEGLKRACKYFQQAAGCFQ--------YLKENFLHAPSPDLSPETLKALSNLMLAQAQECF 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 234 LGLAI-DSPKATLAVKrrLACEMVKYWHQIQESIPEIPVSD-GWgkKHLLFVKWKYVeaKAAAYYFHGLILDEgntEKSH 311
Cdd:pfam03097 184 WEKAInDNKKDSLIAK--LAAQVSELYEEALEALKLSGLIDkEW--ISHVQAKAHHF--KALAQYRQALDDEE---AKKY 254

                         250       260
                  ....*....|....*....|.
gi 1002263446 312 GMAVAALQASEEFLKESKRAS 332
Cdd:pfam03097 255 GEEIARLQLALSLLKEALKSD 275
BRO1_Alix_like_2 cd09247
Protein-interacting Bro1-like domain of an Uncharacterized family of the BRO1_Alix_like ...
 40-397 1.01e-04

Protein-interacting Bro1-like domain of an Uncharacterized family of the BRO1_Alix_like superfamily; This domain family is comprised of uncharacterized proteins. It belongs to the BRO1_Alix_like superfamily which includes the Bro1-like domains of mammalian Alix (apoptosis-linked gene-2 interacting protein X), His-Domain type N23 protein tyrosine phosphatase (HD-PTP, also known as PTPN23), RhoA-binding proteins Rhophilin-1 and -2, Brox, Bro1 and Rim20 (also known as PalA) from Saccharomyces cerevisiae, Ustilago maydis Rim23 (also known as PalC), and related domains. Alix, HD-PTP, Brox, Bro1, Rim20 and Rim23 interact with the ESCRT (Endosomal Sorting Complexes Required for Transport) system. Alix participates in membrane remodeling processes during the budding of enveloped viruses, vesicle budding inside late endosomal multivesicular bodies (MVBs), and the abscission reactions of mammalian cell division. It also functions in apoptosis. HD-PTP and Bro1 function in endosomal trafficking, with HD-PTP having additional functions in cell migration. Rim20 and Rim23 play roles in the response to the external pH via the Rim101 pathway. Bro1-like domains are boomerang-shaped, and part of the domain is a tetratricopeptide repeat (TPR)-like structure. These domains bind components of the ESCRT-III complex: CHMP4 (in the case of Alix, Brox and HD-PTP) and Snf7 (in the case of yeast Bro1 and Rim20). The Bro1-like domains of Alix, HD-PTP, Brox, and Rhophilin can bind human immunodeficiency virus type 1 (HIV-1) nucleocapsid. This family lacks the V-shaped (V) domain found in many members of the BRO1_Alix_like superfamily.


Pssm-ID: 185770  Cd Length: 346  Bit Score: 43.92  E-value: 1.01e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446  40 FSQALAGRLDEASSRLSSLRARvvdmaMQESAAALKPKRRAAARHGSSTANLLQALEDYLPVLLGLVK--EGSELRHGVH 117
Cdd:cd09247    13 FEKTFQARDSLTLEQLKELSLR-----RRAIIESINGSPFIALAIAREKAQYLPYLEGYLPALENLVNhrDKVQLNEQLS 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 118 FVWTNQEDNAEETAmadaWYEVLSVLHLMAMVCLLqanslllprsYGDGYAPRVSEESRR----ATVDVFLKASGYLDCA 193
Cdd:cd09247    88 FRWTSGLGSSKGPK----AFQSDSLRFELGMVLFL----------YGAALRERASEVLPTedfkEAATHLRRAAGVFEFL 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 194 IRQVLP----QISSELRrqlPVDLAEGNLKALSLQALGQGVDMQLGLAIDSPKaTLAVKRRLACEMvkyWHQIQESIPEI 269
Cdd:cd09247   154 AHDELPrlrgALSADER---PPECTPSLALAMSLLCLAEAQAVTARKAEEKGT-SPSLLAKLHYGA---TQFLEEAKNVL 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002263446 270 PVSDGWGK----KHLLFVKWKYVEAKAAAYYFHGLILDEgntEKSHGMAVAALQASEEFLKESKRASEafhatpptSRSP 345
Cdd:cd09247   227 RSLATDLKdldpRFLRFISSCIALHEARSQLYLARRLKE---AGHIGVAVGVLREALRNLKKKLPGSD--------ISSP 295

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1002263446 346 TPFGTakyMLDKIPKDASSKVKINQDLYTQERVIGTPPPLPDFALALKPDDY 397
Cdd:cd09247   296 VIFRD---ERAEVATLLQKYEKENEVIYFEKVPDIDELPLPEGKVIVKPVPY 344
BRO1_Brox_like cd09243
Protein-interacting Bro1-like domain of human Brox1 and related proteins; This family contains ...
 91-148 2.09e-03

Protein-interacting Bro1-like domain of human Brox1 and related proteins; This family contains the Bro1-like domain of a single-domain protein, human Brox, and related domains. It belongs to the BRO1_Alix_like superfamily which also includes the Bro1-like domains of mammalian Alix (apoptosis-linked gene-2 interacting protein X), His-Domain type N23 protein tyrosine phosphatase (HD-PTP, also known as PTPN23), RhoA-binding proteins Rhophilin-1 and -2, Bro1 and Rim20 (also known as PalA) from Saccharomyces cerevisiae, Ustilago maydis Rim23 (also known as PalC), and related domains. Alix, HD-PTP, Brox, Bro1, Rim20, and Rim23, interact with the ESCRT (Endosomal Sorting Complexes Required for Transport) system. Bro1-like domains are boomerang-shaped, and part of the domain is a tetratricopeptide repeat (TPR)-like structure. Bro1-like domains bind components of the ESCRT-III complex: CHMP4 in the case of Brox. Human Brox can bind to human immunodeficiency virus type 1 (HIV-1) nucleocapsid. In addition to a Bro1-like domain, Brox also has a C-terminal thioester-linkage site for isoprenoid lipids (CaaX motif). This family lacks the V-shaped (V) domain found in many members of the BRO1_Alix_like superfamily.


Pssm-ID: 185766  Cd Length: 353  Bit Score: 40.02  E-value: 2.09e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1002263446  91 LLQALEDYLPVLLGLVKEG------SELRHGVHFVWTNQEDNAEETAMADAWYEVLSVLHLMAM 148
Cdd:cd09243    54 VKTAFNAYLSLLQGFILALdgktqeSKLRYLINFKWTDSLLGNEPSVQQDAIFELASMLFNVAL 117
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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