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Conserved domains on  [gi|1034649363|ref|XP_016866075|]
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forkhead box protein O3 isoform X2 [Homo sapiens]

Protein Classification

FOXO_KIX_bdg and FOXO-TAD domain-containing protein( domain architecture ID 10891529)

protein containing domains FH, FOXO_KIX_bdg, and FOXO-TAD

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FOXO_KIX_bdg pfam16675
KIX-binding domain of forkhead box O, CR2; FOXO_KIX_bd is the first part of the region of ...
 212-291 3.93e-29

KIX-binding domain of forkhead box O, CR2; FOXO_KIX_bd is the first part of the region of transcription factor forkhead box O family proteins that binds to the CREB-binding proteins via the KIX domain. Coactivator CBP/p300 is recruited by FOXO3 via the binding of this domain as well as the simultaneous binding of the more C-terminal TAD domain.


:

Pssm-ID: 465228  Cd Length: 80  Bit Score: 109.05  E-value: 3.93e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034649363 212 STVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGN-QTLQDLLTSDSLSHSDVmMTQSDPLMSQASTAVSAQNSRRNVM 290
Cdd:pfam16675   1 KSIYGQPSMNSLRQMPMQTIQENKQSSFGSMSQYGPaGLLQELLTSDSYPHSDI-LTQVDPLMSQASGRVLSQNHRQNSV 79


                  .
gi 1034649363 291 L 291
Cdd:pfam16675  80 M 80
FOXO-TAD pfam16676
Transactivation domain of FOXO protein family; TAD is a promiscuous binding domain that ...
 386-424 4.53e-19

Transactivation domain of FOXO protein family; TAD is a promiscuous binding domain that mediates the association of the transcription factor FOXO with the coactivator CBP/p300. Both this domain and the FOXO-KIX_bd family pfam16675 bind simultaneously the KIX domain of CBP/p300. Coactivator CBP/p300 is recruited by FOXO3 though binding to these two regions. The promiscuity of the TAD is further evidenced by that the finding that they also bind the TAZ1 and TAZ2 domains of CBP/p300.


:

Pssm-ID: 435506  Cd Length: 40  Bit Score: 80.07  E-value: 4.53e-19
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 1034649363 386 KFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDS 424
Cdd:pfam16676   1 RLPSDLDLDMFQGSLECDVDSIIRNDLMDGDGLDFNFDS 39
FH_FOX super family cl00061
Forkhead (FH) domain found in Forkhead box (FOX) family of transcription factors and similar ...
 1-25 1.56e-09

Forkhead (FH) domain found in Forkhead box (FOX) family of transcription factors and similar proteins; The FOX family comprises diverse tissue- and cell type-specific transcription factors with an evolutionary conserved "Forkhead (FH)" or "winged helix" DNA-binding domain. FH is named for the Drosophila fork head protein, a transcription factor which promotes terminal rather than segmental development. The structure of the FH domain contains 2 flexible loops or "wings" in the C-terminal region, hence the term winged helix. FOX transcription factors recognize the core sequence 5'-(A/C)AA(C/T)A-3'. They participate in a variety of cellular processes, such as cell cycle progression, proliferation, differentiation, migration, metabolism, and DNA damage response. Their expression can be regulated by multiple factors, and they can act as co-activators and/or transcriptional repressors. Fifty FOX-encoding genes in humans have been categorized into 19 subfamilies based on protein sequence homology (FOXA to FOXS).


The actual alignment was detected with superfamily member cd20063:

Pssm-ID: 469596 [Multi-domain]  Cd Length: 88  Bit Score: 54.67  E-value: 1.56e-09
                          10        20
                  ....*....|....*....|....*
gi 1034649363   1 MRVQNEGTGKSSWWIINPDGGKSGK 25
Cdd:cd20063    64 IRVQNEGTGKSSWWMLNPEGGKTGK 88
 
Name Accession Description Interval E-value
FOXO_KIX_bdg pfam16675
KIX-binding domain of forkhead box O, CR2; FOXO_KIX_bd is the first part of the region of ...
 212-291 3.93e-29

KIX-binding domain of forkhead box O, CR2; FOXO_KIX_bd is the first part of the region of transcription factor forkhead box O family proteins that binds to the CREB-binding proteins via the KIX domain. Coactivator CBP/p300 is recruited by FOXO3 via the binding of this domain as well as the simultaneous binding of the more C-terminal TAD domain.


Pssm-ID: 465228  Cd Length: 80  Bit Score: 109.05  E-value: 3.93e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034649363 212 STVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGN-QTLQDLLTSDSLSHSDVmMTQSDPLMSQASTAVSAQNSRRNVM 290
Cdd:pfam16675   1 KSIYGQPSMNSLRQMPMQTIQENKQSSFGSMSQYGPaGLLQELLTSDSYPHSDI-LTQVDPLMSQASGRVLSQNHRQNSV 79


                  .
gi 1034649363 291 L 291
Cdd:pfam16675  80 M 80
FOXO-TAD pfam16676
Transactivation domain of FOXO protein family; TAD is a promiscuous binding domain that ...
 386-424 4.53e-19

Transactivation domain of FOXO protein family; TAD is a promiscuous binding domain that mediates the association of the transcription factor FOXO with the coactivator CBP/p300. Both this domain and the FOXO-KIX_bd family pfam16675 bind simultaneously the KIX domain of CBP/p300. Coactivator CBP/p300 is recruited by FOXO3 though binding to these two regions. The promiscuity of the TAD is further evidenced by that the finding that they also bind the TAZ1 and TAZ2 domains of CBP/p300.


Pssm-ID: 435506  Cd Length: 40  Bit Score: 80.07  E-value: 4.53e-19
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 1034649363 386 KFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDS 424
Cdd:pfam16676   1 RLPSDLDLDMFQGSLECDVDSIIRNDLMDGDGLDFNFDS 39
FH_FOXO6 cd20063
Forkhead (FH) domain found in Forkhead box protein O6 (FOXO6) and similar proteins; FOXO6 acts ...
 1-25 1.56e-09

Forkhead (FH) domain found in Forkhead box protein O6 (FOXO6) and similar proteins; FOXO6 acts as a transcriptional activator that may play an important role on tumor invasion, metastasis and prognosis. The FH domain is a winged helix DNA-binding domain. All FOXOs bind to the consensus sequence 5'-GTAAACAA-3', known as the DAF-16 family member-binding element, which includes the core sequence 5'-(A/C)AA(C/T)A-3' recognized by all FOX family members.


Pssm-ID: 410837 [Multi-domain]  Cd Length: 88  Bit Score: 54.67  E-value: 1.56e-09
                          10        20
                  ....*....|....*....|....*
gi 1034649363   1 MRVQNEGTGKSSWWIINPDGGKSGK 25
Cdd:cd20063    64 IRVQNEGTGKSSWWMLNPEGGKTGK 88
 
Name Accession Description Interval E-value
FOXO_KIX_bdg pfam16675
KIX-binding domain of forkhead box O, CR2; FOXO_KIX_bd is the first part of the region of ...
 212-291 3.93e-29

KIX-binding domain of forkhead box O, CR2; FOXO_KIX_bd is the first part of the region of transcription factor forkhead box O family proteins that binds to the CREB-binding proteins via the KIX domain. Coactivator CBP/p300 is recruited by FOXO3 via the binding of this domain as well as the simultaneous binding of the more C-terminal TAD domain.


Pssm-ID: 465228  Cd Length: 80  Bit Score: 109.05  E-value: 3.93e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034649363 212 STVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGN-QTLQDLLTSDSLSHSDVmMTQSDPLMSQASTAVSAQNSRRNVM 290
Cdd:pfam16675   1 KSIYGQPSMNSLRQMPMQTIQENKQSSFGSMSQYGPaGLLQELLTSDSYPHSDI-LTQVDPLMSQASGRVLSQNHRQNSV 79


                  .
gi 1034649363 291 L 291
Cdd:pfam16675  80 M 80
FOXO-TAD pfam16676
Transactivation domain of FOXO protein family; TAD is a promiscuous binding domain that ...
 386-424 4.53e-19

Transactivation domain of FOXO protein family; TAD is a promiscuous binding domain that mediates the association of the transcription factor FOXO with the coactivator CBP/p300. Both this domain and the FOXO-KIX_bd family pfam16675 bind simultaneously the KIX domain of CBP/p300. Coactivator CBP/p300 is recruited by FOXO3 though binding to these two regions. The promiscuity of the TAD is further evidenced by that the finding that they also bind the TAZ1 and TAZ2 domains of CBP/p300.


Pssm-ID: 435506  Cd Length: 40  Bit Score: 80.07  E-value: 4.53e-19
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 1034649363 386 KFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDS 424
Cdd:pfam16676   1 RLPSDLDLDMFQGSLECDVDSIIRNDLMDGDGLDFNFDS 39
FH_FOXO6 cd20063
Forkhead (FH) domain found in Forkhead box protein O6 (FOXO6) and similar proteins; FOXO6 acts ...
 1-25 1.56e-09

Forkhead (FH) domain found in Forkhead box protein O6 (FOXO6) and similar proteins; FOXO6 acts as a transcriptional activator that may play an important role on tumor invasion, metastasis and prognosis. The FH domain is a winged helix DNA-binding domain. All FOXOs bind to the consensus sequence 5'-GTAAACAA-3', known as the DAF-16 family member-binding element, which includes the core sequence 5'-(A/C)AA(C/T)A-3' recognized by all FOX family members.


Pssm-ID: 410837 [Multi-domain]  Cd Length: 88  Bit Score: 54.67  E-value: 1.56e-09
                          10        20
                  ....*....|....*....|....*
gi 1034649363   1 MRVQNEGTGKSSWWIINPDGGKSGK 25
Cdd:cd20063    64 IRVQNEGTGKSSWWMLNPEGGKTGK 88
FH_FOXO1 cd20060
Forkhead (FH) domain found in Forkhead box protein O1 (FOXO1); FOXO1, also called Forkhead box ...
 1-26 3.72e-08

Forkhead (FH) domain found in Forkhead box protein O1 (FOXO1); FOXO1, also called Forkhead box protein O1A or Forkhead in rhabdomyosarcoma (FKHR), is a transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. It binds to the insulin response element (IRE) with the consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with the consensus sequence 5'-TT[G/A]TTTAC-3'. The FH domain is a winged helix DNA-binding domain.


Pssm-ID: 410834 [Multi-domain]  Cd Length: 99  Bit Score: 51.00  E-value: 3.72e-08
                          10        20
                  ....*....|....*....|....*.
gi 1034649363   1 MRVQNEGTGKSSWWIINPDGGKSGKA 26
Cdd:cd20060    74 IRVQNEGTGKSSWWMLNPEGGKSGKS 99
FH_FOXO3 cd20061
Forkhead (FH) domain found in Forkhead box protein O3 (FOXO3); FOXO3, also called AF6q21 ...
 1-19 1.05e-06

Forkhead (FH) domain found in Forkhead box protein O3 (FOXO3); FOXO3, also called AF6q21 protein or Forkhead in rhabdomyosarcoma-like 1 (FKHRL1), is a transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. It recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. The FH domain is a winged helix DNA-binding domain. All FOXOs bind to the consensus sequence 5'-GTAAACAA-3', known as the DAF-16 family member-binding element, which includes the core sequence 5'-(A/C)AA(C/T)A-3' recognized by all FOX family members.


Pssm-ID: 410835 [Multi-domain]  Cd Length: 83  Bit Score: 46.59  E-value: 1.05e-06
                          10
                  ....*....|....*....
gi 1034649363   1 MRVQNEGTGKSSWWIINPD 19
Cdd:cd20061    65 IRVQNEGTGKSSWWMINPD 83
FH_FOXO4 cd20062
Forkhead (FH) domain found in Forkhead box protein O4 (FOXO4) and similar proteins; FOXO4, ...
 1-19 5.10e-04

Forkhead (FH) domain found in Forkhead box protein O4 (FOXO4) and similar proteins; FOXO4, also called Fork head domain transcription factor AFX1, is a transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. The FH domain is a winged helix DNA-binding domain. All FOXOs bind to the consensus sequence 5'-GTAAACAA-3', known as the DAF-16 family member-binding element, which includes the core sequence 5'-(A/C)AA(C/T)A-3' recognized by all FOX family members.


Pssm-ID: 410836 [Multi-domain]  Cd Length: 86  Bit Score: 38.92  E-value: 5.10e-04
                          10
                  ....*....|....*....
gi 1034649363   1 MRVQNEGTGKSSWWIINPD 19
Cdd:cd20062    68 IKVHNEATGKSSWWMLNPE 86
FH_FOXO cd20032
Forkhead (FH) domain found in the Forkhead box protein O (FOXO) subfamily; The FOXO subfamily ...
 1-17 7.09e-04

Forkhead (FH) domain found in the Forkhead box protein O (FOXO) subfamily; The FOXO subfamily includes several winged helix transcription factors: FOXO1, FOXO3, FOXO4 and FOXO6. FOXO transcription factors are involved in the regulation of longevity phenomenon via insulin and insulin-like growth factor signaling. All FOXOs bind to the consensus sequence 5'-GTAAACAA-3', known as the DAF-16 family member-binding element, which includes the core sequence 5'-(A/C)AA(C/T)A-3' recognized by all FOX family members. FOXO1, also called Forkhead box protein O1A or Forkhead in rhabdomyosarcoma (FKHR), is a transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. FOXO3, also called AF6q21 protein or Forkhead in rhabdomyosarcoma-like 1 (FKHRL1), is a transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. It recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. FOXO4, also called Fork head domain transcription factor AFX1, is a transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO6 acts as a transcriptional activator that may play an important role on tumor invasion, metastasis, and prognosis. The FH domain is a winged helix DNA-binding domain.


Pssm-ID: 410806 [Multi-domain]  Cd Length: 80  Bit Score: 38.43  E-value: 7.09e-04
                          10
                  ....*....|....*..
gi 1034649363   1 MRVQNEGTGKSSWWIIN 17
Cdd:cd20032    64 VRVQNEGTGKSSWWTLN 80
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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