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Conserved domains on  [gi|1034651767|ref|XP_016866824|]
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sperm acrosome membrane-associated protein 1 isoform X2 [Homo sapiens]

Protein Classification

SPACA1 domain-containing protein( domain architecture ID 10194958)

SPACA1 domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SPACA1 cd13783
Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is ...
 1-193 2.26e-134

Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is localized to the acrosome of spermatozoa. The acrosome is an organelle transformed from the Golgi apparatus to form a cap over the anterior portion of the spermatozoa head, which contains the sperm nucleus. Mammalian acrosomes contain digestive enzymes that degrade the ovum outer membrane (zona pellucida) to allow fusion of the sperm and ovum nuclei via the acrosomal reaction. In mammals, the acrosome releases hyaluronidase and acrosin. Antibodies generated against recombinant SPACA1 have been shown to inhibit human sperm binding and membrane fusion in vitro vs. zona-free hamster ova. Male mice lacking SPACA1 are infertile, and exhibit globozoospermia-like misformed sperm heads. SPACA1 content has been reported to be diminished in a comparison of round-headed vs normal spermatozoa.


:

Pssm-ID: 260101  Cd Length: 248  Bit Score: 376.85  E-value: 2.26e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034651767   1 MCTVTCGIGVREVILTNGCPGGESKCVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSII 80
Cdd:cd13783    56 MCTVTCGIGIREVILTNGCPGGESKCVVRVEECRGPVDCGWGKPISESLSSVRLACIHISPENRFKYVWKLLRPDQQAVI 135

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034651767  81 LVNDSAILEVRKESHPLAFECDTLDNNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIII 160
Cdd:cd13783   136 LPNDSAVLEVHRETHPVAFECDTLDNNEIVASVKFTVYTTSELQMRRSSRPDTDAVLIFVLTIGVIICIFVIFVLIFIII 215

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1034651767 161 NWAAVKAFWGAKASTPEVQSEQSSVRYKDSTSL 193
Cdd:cd13783   216 NWAAVKSFWGSKASTTEIQSELSSMRYKDSTSL 248
 
Name Accession Description Interval E-value
SPACA1 cd13783
Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is ...
 1-193 2.26e-134

Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is localized to the acrosome of spermatozoa. The acrosome is an organelle transformed from the Golgi apparatus to form a cap over the anterior portion of the spermatozoa head, which contains the sperm nucleus. Mammalian acrosomes contain digestive enzymes that degrade the ovum outer membrane (zona pellucida) to allow fusion of the sperm and ovum nuclei via the acrosomal reaction. In mammals, the acrosome releases hyaluronidase and acrosin. Antibodies generated against recombinant SPACA1 have been shown to inhibit human sperm binding and membrane fusion in vitro vs. zona-free hamster ova. Male mice lacking SPACA1 are infertile, and exhibit globozoospermia-like misformed sperm heads. SPACA1 content has been reported to be diminished in a comparison of round-headed vs normal spermatozoa.


Pssm-ID: 260101  Cd Length: 248  Bit Score: 376.85  E-value: 2.26e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034651767   1 MCTVTCGIGVREVILTNGCPGGESKCVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSII 80
Cdd:cd13783    56 MCTVTCGIGIREVILTNGCPGGESKCVVRVEECRGPVDCGWGKPISESLSSVRLACIHISPENRFKYVWKLLRPDQQAVI 135

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034651767  81 LVNDSAILEVRKESHPLAFECDTLDNNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIII 160
Cdd:cd13783   136 LPNDSAVLEVHRETHPVAFECDTLDNNEIVASVKFTVYTTSELQMRRSSRPDTDAVLIFVLTIGVIICIFVIFVLIFIII 215

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1034651767 161 NWAAVKAFWGAKASTPEVQSEQSSVRYKDSTSL 193
Cdd:cd13783   216 NWAAVKSFWGSKASTTEIQSELSSMRYKDSTSL 248
 
Name Accession Description Interval E-value
SPACA1 cd13783
Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is ...
 1-193 2.26e-134

Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is localized to the acrosome of spermatozoa. The acrosome is an organelle transformed from the Golgi apparatus to form a cap over the anterior portion of the spermatozoa head, which contains the sperm nucleus. Mammalian acrosomes contain digestive enzymes that degrade the ovum outer membrane (zona pellucida) to allow fusion of the sperm and ovum nuclei via the acrosomal reaction. In mammals, the acrosome releases hyaluronidase and acrosin. Antibodies generated against recombinant SPACA1 have been shown to inhibit human sperm binding and membrane fusion in vitro vs. zona-free hamster ova. Male mice lacking SPACA1 are infertile, and exhibit globozoospermia-like misformed sperm heads. SPACA1 content has been reported to be diminished in a comparison of round-headed vs normal spermatozoa.


Pssm-ID: 260101  Cd Length: 248  Bit Score: 376.85  E-value: 2.26e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034651767   1 MCTVTCGIGVREVILTNGCPGGESKCVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSII 80
Cdd:cd13783    56 MCTVTCGIGIREVILTNGCPGGESKCVVRVEECRGPVDCGWGKPISESLSSVRLACIHISPENRFKYVWKLLRPDQQAVI 135

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034651767  81 LVNDSAILEVRKESHPLAFECDTLDNNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIII 160
Cdd:cd13783   136 LPNDSAVLEVHRETHPVAFECDTLDNNEIVASVKFTVYTTSELQMRRSSRPDTDAVLIFVLTIGVIICIFVIFVLIFIII 215

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1034651767 161 NWAAVKAFWGAKASTPEVQSEQSSVRYKDSTSL 193
Cdd:cd13783   216 NWAAVKSFWGSKASTTEIQSELSSMRYKDSTSL 248
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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