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Conserved domains on  [gi|1720408094|ref|XP_030109255|]
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glycoprotein endo-alpha-1,2-mannosidase-like protein isoform X1 [Mus musculus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GH99_GH71_like super family cl01529
Glycoside hydrolase families 71, 99, and related domains; This superfamily of glycoside ...
 100-215 2.75e-60

Glycoside hydrolase families 71, 99, and related domains; This superfamily of glycoside hydrolases contains families GH71 and GH99 (following the CAZY nomenclature), as well as other members with undefined function and specificity.


The actual alignment was detected with superfamily member cd11574:

Pssm-ID: 470237  Cd Length: 338  Bit Score: 193.30  E-value: 2.75e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720408094 100 LHAFYYSWYGSPRREGHYIHWDHVMVPHWDpkISASYPRGRHSPPDDLGSSFYPELGPYSSRDPDVLREHMTQLKEAAIG 179
Cdd:cd11574     1 VHIFYYAWYGNPEFDGKYGHWNHKILPHWD--IAKKYPQGRHDPPDDIGSNFYPKLGPYSSSDPSVIDDHMKQIREAGIG 78

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1720408094 180 VLVLSWYPPGMADDNGEPTDDLVPAILDTAHQYNIQ 215
Cdd:cd11574    79 VVVVSWYGPGSSDDNGKPSDDTIPLLLDIAHEYGLK 114
 
Name Accession Description Interval E-value
GH99 cd11574
Glycoside hydrolase family 99, an endo-alpha-1,2-mannosidase; This family of glycoside ...
 100-215 2.75e-60

Glycoside hydrolase family 99, an endo-alpha-1,2-mannosidase; This family of glycoside hydrolases 99 (following the CAZY nomenclature) includes endo-alpha-1,2-mannosidase (EC 3.2.1.130), which is an important membrane-associated eukaryotic enzyme involved in the maturation of N-linked glycans. Specifically, it cleaves mannoside linkages internal to N-linked glycan chains by hydrolyzing an alpha-1,2-mannosidic bond between a glucose-substituted mannose and the remainder of the chain. The biological function and significance of the soluble bacterial orthologs, which may have obtained the genes via horizontal transfer, is not clear.


Pssm-ID: 211415  Cd Length: 338  Bit Score: 193.30  E-value: 2.75e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720408094 100 LHAFYYSWYGSPRREGHYIHWDHVMVPHWDpkISASYPRGRHSPPDDLGSSFYPELGPYSSRDPDVLREHMTQLKEAAIG 179
Cdd:cd11574     1 VHIFYYAWYGNPEFDGKYGHWNHKILPHWD--IAKKYPQGRHDPPDDIGSNFYPKLGPYSSSDPSVIDDHMKQIREAGIG 78

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1720408094 180 VLVLSWYPPGMADDNGEPTDDLVPAILDTAHQYNIQ 215
Cdd:cd11574    79 VVVVSWYGPGSSDDNGKPSDDTIPLLLDIAHEYGLK 114
Glyco_hydro_99 pfam16317
Glycosyl hydrolase family 99; This domain, around 350 residues, is mainly found in some ...
 100-215 1.02e-47

Glycosyl hydrolase family 99; This domain, around 350 residues, is mainly found in some uncharacterized proteins from bacteroides to human. Some proteins in this family, annotated as endo-alpha-mannosidases cleave mannoside linkages internally within an N-linked glycan chain, short circuiting the classical N-glycan biosynthetic pathway. This domain reveals a (beta-alpha)(8) barrel fold in which the catalytic centre is present in a long substrate-binding groove, consistent with cleavage within the N-glycan chain, providing a foundation upon which to develop new enzyme inhibitors targeting the hijacking of N-glycan synthesis in viral disease and cancer.


Pssm-ID: 435273  Cd Length: 341  Bit Score: 160.83  E-value: 1.02e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720408094 100 LHAFYYSWYGSPRREGHYIHWDHVMVPHWDPKISAS-YPRGRHSPPDDLGSSFYPELGPYSSRDPDVLREHMTQLKEAAI 178
Cdd:pfam16317   6 LHVFYYSWYGNPQFDGKYQHWNHPVLEHWDPRIGKLnYPGARHGPPDDIGSNFYPELGSYSSRDPEIIETHMRMMRSASI 85

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1720408094 179 GVLVLSWYppgmaDDNGEPTdDLVPAILDTAHQYNIQ 215
Cdd:pfam16317  86 GVLSVSWY-----GENDEAT-RSVPTILDKAAKYGLK 116
 
Name Accession Description Interval E-value
GH99 cd11574
Glycoside hydrolase family 99, an endo-alpha-1,2-mannosidase; This family of glycoside ...
 100-215 2.75e-60

Glycoside hydrolase family 99, an endo-alpha-1,2-mannosidase; This family of glycoside hydrolases 99 (following the CAZY nomenclature) includes endo-alpha-1,2-mannosidase (EC 3.2.1.130), which is an important membrane-associated eukaryotic enzyme involved in the maturation of N-linked glycans. Specifically, it cleaves mannoside linkages internal to N-linked glycan chains by hydrolyzing an alpha-1,2-mannosidic bond between a glucose-substituted mannose and the remainder of the chain. The biological function and significance of the soluble bacterial orthologs, which may have obtained the genes via horizontal transfer, is not clear.


Pssm-ID: 211415  Cd Length: 338  Bit Score: 193.30  E-value: 2.75e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720408094 100 LHAFYYSWYGSPRREGHYIHWDHVMVPHWDpkISASYPRGRHSPPDDLGSSFYPELGPYSSRDPDVLREHMTQLKEAAIG 179
Cdd:cd11574     1 VHIFYYAWYGNPEFDGKYGHWNHKILPHWD--IAKKYPQGRHDPPDDIGSNFYPKLGPYSSSDPSVIDDHMKQIREAGIG 78

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1720408094 180 VLVLSWYPPGMADDNGEPTDDLVPAILDTAHQYNIQ 215
Cdd:cd11574    79 VVVVSWYGPGSSDDNGKPSDDTIPLLLDIAHEYGLK 114
Glyco_hydro_99 pfam16317
Glycosyl hydrolase family 99; This domain, around 350 residues, is mainly found in some ...
 100-215 1.02e-47

Glycosyl hydrolase family 99; This domain, around 350 residues, is mainly found in some uncharacterized proteins from bacteroides to human. Some proteins in this family, annotated as endo-alpha-mannosidases cleave mannoside linkages internally within an N-linked glycan chain, short circuiting the classical N-glycan biosynthetic pathway. This domain reveals a (beta-alpha)(8) barrel fold in which the catalytic centre is present in a long substrate-binding groove, consistent with cleavage within the N-glycan chain, providing a foundation upon which to develop new enzyme inhibitors targeting the hijacking of N-glycan synthesis in viral disease and cancer.


Pssm-ID: 435273  Cd Length: 341  Bit Score: 160.83  E-value: 1.02e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720408094 100 LHAFYYSWYGSPRREGHYIHWDHVMVPHWDPKISAS-YPRGRHSPPDDLGSSFYPELGPYSSRDPDVLREHMTQLKEAAI 178
Cdd:pfam16317   6 LHVFYYSWYGNPQFDGKYQHWNHPVLEHWDPRIGKLnYPGARHGPPDDIGSNFYPELGSYSSRDPEIIETHMRMMRSASI 85

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1720408094 179 GVLVLSWYppgmaDDNGEPTdDLVPAILDTAHQYNIQ 215
Cdd:pfam16317  86 GVLSVSWY-----GENDEAT-RSVPTILDKAAKYGLK 116
GH99_GH71_like_3 cd11575
Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside ...
 96-194 4.90e-08

Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.


Pssm-ID: 211416  Cd Length: 376  Bit Score: 52.73  E-value: 4.90e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720408094  96 VYSDLHAFYYSwygspRREGHYIHWdHVMVPHWDPkiSASYPRGRHsppdDLGSSFYPELGPYSSRDPDVLREHMTQLKE 175
Cdd:cd11575     9 VYAHYMPWFET-----RPDDGKWGW-HWTMANFDP--DHIDASGKR----QIASHYYPLIGPYSSGDPDVIEYQLLLMKL 76

                          90       100
                  ....*....|....*....|
gi 1720408094 176 AAI-GVLVlSWYppGMADDN 194
Cdd:cd11575    77 AGIdGVIV-DWY--GTGHFS 93
GH99_GH71_like_1 cd11578
Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside ...
 102-193 1.52e-04

Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.


Pssm-ID: 211419  Cd Length: 313  Bit Score: 42.01  E-value: 1.52e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720408094 102 AFYYSWYGSprreghYIHWDhvmvphwdpkisasyprgrhsppddLGSSFYPELGPYSSRDPDVLREHMTQLKEAAIGVL 181
Cdd:cd11578     3 AYYYNWTSS------GLDWN-------------------------KKYPEEPLLGEYDALDPAVIEQHIDWADQAGIDFF 51

                          90
                  ....*....|..
gi 1720408094 182 VLSWYPPGMADD 193
Cdd:cd11578    52 IVSWWGPDNDNV 63
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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