BioProject

Library construction and genome sequencing of S. reilianum strain SRZ2 was done at 454 Life Sciences (USA). More...

Library construction and genome sequencing of S. reilianum strain SRZ2 was done at 454 Life Sciences (USA). As a first step a whole genome shotgun random library was constructed and sequenced using the Genome Sequencer 20 (Roche). In a second step, the genome was sequenced from a newly generated paired-end library of S. reilianum strain SRZ2 using the Genome Sequencer FLX (Roche). In a third step, the scaffold-assembly was verified by generating an NheI-based Optical Map (OpGen). Optical Mapping resulted in the assembly of 23 chromosomes on which 99.75% of the genome assembly could be placed. The total genome size based on Optical Mapping is estimated to be 18.7 Mb. Thus, the assembly contains 97 % of total sequence information. Less...