BioSample

Protocols: Animals were sedated with isoflurane and immediately culled by cervical dislocation at ZT2 or ZT14. Retina, lung and liver tissues was dissected and snap frozen immediately. For SCN samples, punches were collected from 1 mm thick brain slices containing a sample corer. All samples were stored at -80°C until RNA extraction. For retina, lung and liver samples total RNA was extracted from < 5 mg frozen tissue using the Qiagen RNeasy Mini kit (Qiagen 74104). For SCN, total RNA was extracted from snap-frozen brain punches using the Qiagen RNeasy Micro kit (Qiagen 74004). All samples were quantified using a 4200 TapeStation (Agilent Technologies). For retina, lung and liver samples, cDNA libraries were generated using the Illumina Stranded mRNA Prep Ligation kit (Illumina, Inc. 20040532). For SCN samples, cDNA libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit V3 - Pico Input Mammalian (Takara Bio 634485).