Ebola virus Yambuku-Mayinga (Hartley Guinea pig-ad... - BioSample

Ebola virus Yambuku-Mayinga (Hartley Guinea pig-adapted, p2 Master stock)

Organism: Zaire ebolavirus
Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Filoviridae; Orthoebolavirus; Orthoebolavirus zairense

Attributes

isolate	IRF0147
collected by	Centers for Disease Control and Prevention
collection date	1976
geographic location	Democratic Republic of the Congo
host	Homo sapiens
host disease	Ebola Virus Disease
isolation source	serum
latitude and longitude	n/a
host disease outcome	Non-survivor
host sex	female
passage history	Vero 76 passage 1; Strain 13 Guinea pig pass 4; Vero 76 pass 1; Hartley Guinea pig pass 3; Vero pass 1; Vero E6 pass 1
lab host	Vero C1008 (E6) cells
organism modifier note	harvest date: 30-Dec-2014; passaged 2x in cell culture following isolation
passage_date	24-Dec-2014 to 30-Dec-2014
laboratory_host	VERO C1008 (E6) cells
stock_production	Integrated Research Facility (Postnikova, E.N.; Sword, J.M.; Jahrling, P.B.; Hensley, L.E.; Honko, A.N.)
extraction_method	TrizolLS

Description: The Hartley guinea pig-adapted EBOV (full designation: Ebola virus/UTMB/C.porcellus-lab/COD/1976/Yambuku-Mayinga-GPA, abbreviation: GPA-EBOV/Yam-May) was obtained from Dr. Thomas Geisbert (University of Texas Medical Branch at Galveston, Galveston, TX, USA). VERO C1008 (E6) cells, Kidney (African green monkey), Working Cell Bank, NR-596 were obtained through BEI Resources (National Institute of Allergy and Infectious diseases [NIAID], National Institutes of Health [NIH], Manassas, VA). The IRF0147_EBOV Master stock was propagated using Minimum Essential Medium-alpha, GlutaMAX, no nucleosides (Gibco, ThermoFisher Scientific) supplemented with 2% US-origin, certified, heat-inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific). Following harvest, HI-FBS was QS'd to 10% final concentration prior to cryopreservation.