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NIH_MGC_100

Identifiers
BioSample: SAMN00164142; EST: LIBEST_009631
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissuehepatocellular carcinoma, cell line
lab hostDH10B (phage-resistant)
tissueliver
vectorpOTB7
v_typeplasmid (chloramphenicol resistant, grown at 30C)
re_1XhoI
re_2EcoRI
Description

cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

Submission
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Inge Arlart; 2001-07-16
Accession:
SAMN00164142
ID:
164142

Nucleotide

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