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Knowles Solter mouse blastocyst B1

Identifiers
BioSample: SAMN00155449; EST: LIBEST_000850
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
strainC57BL/6J x DBA/2J F1
tissueblastocyst
development stageembryo (pre-implantation)
lab hostDH10B
tissueembryo
vectorpSPORT
v_typephagemid (ampicillin resistant)
re_1NotI
re_2SalI
Description

Cloned unidirectionally from mRNA prepared from 800 blastocysts. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies). Two different size selections: B1 (larger inserts) and B3.

Submission
Washington University School of MedicineP, Marra M/Mouse EST Project; 1997-04-25
Accession:
SAMN00155449
ID:
155449

Nucleotide

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