NICHD_MM_Hyp1

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Result Filters

Organism: Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus

Attributes

lab host	DH10B (phage-resistant)
tissue	brain - normal mediobasal hypothalamus
vector	pDNR-LIB
v_type	plasmid (chloramphenicol resistant, grown at 30C)
re_1	SfiI (ggccattatggcc)
re_2	SfiI (ggccgcctcggcc)

Description: Non-normalized full-length enriched library 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.08 kb (range .73-1.37 kb). 13/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).Corp.