BioSample

Library is oligo-dT primed and directionally cloned. Granulosa lutein cells aspirated from preovulatory folicles of normal cycling women undergoing ovulation induction for infertility due to male factor and normal doners. The cells were from follicles stimulated with Lupron, FSH and hCG. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 2.23 kb (range 1.0-4.5 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).